AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene03121

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene03121.t1 MTR_2g017590 92.251 271 20 1 1 271 1 270 0.0 507
MS.gene03121.t1 MTR_2g017620 93.680 269 15 1 1 267 1 269 0.0 506
MS.gene03121.t1 MTR_2g017620 94.902 255 13 0 13 267 1 255 2.58e-178 491
MS.gene03121.t1 MTR_2g017570 79.401 267 55 0 5 271 7 273 1.76e-159 444
MS.gene03121.t1 MTR_2g017610 79.401 267 55 0 5 271 7 273 2.34e-159 444
MS.gene03121.t1 MTR_8g087720 74.231 260 67 0 5 264 7 266 2.26e-144 405
MS.gene03121.t1 MTR_8g087710 67.176 262 86 0 10 271 13 274 2.25e-130 370
MS.gene03121.t1 MTR_2g017620 95.745 188 8 0 80 267 1 188 1.26e-129 365
MS.gene03121.t1 MTR_2g017660 65.074 272 86 2 1 263 1 272 6.83e-129 367
MS.gene03121.t1 MTR_8g087720 73.846 195 51 0 5 199 7 201 6.90e-102 295
MS.gene03121.t1 MTR_4g084880 57.627 236 98 1 32 265 31 266 1.23e-97 287
MS.gene03121.t1 MTR_8g087720 71.348 178 51 0 5 182 7 184 1.35e-88 262
MS.gene03121.t1 MTR_5g079570 52.610 249 109 3 6 251 12 254 4.56e-88 263
MS.gene03121.t1 MTR_3g008920 53.138 239 109 2 16 254 8 243 4.57e-85 255
MS.gene03121.t1 MTR_4g006730 49.799 249 118 2 15 260 54 298 1.04e-73 228
MS.gene03121.t1 MTR_2g104940 49.206 252 119 3 14 260 56 303 9.27e-72 223
MS.gene03121.t1 MTR_7g010650 47.137 227 118 2 36 262 37 261 1.15e-69 216
MS.gene03121.t1 MTR_1g097840 49.573 234 107 3 32 257 70 300 3.42e-67 211
MS.gene03121.t1 MTR_5g063930 44.737 266 132 6 1 258 1 259 6.16e-67 209
MS.gene03121.t1 MTR_4g006630 52.093 215 96 2 49 260 1 211 1.28e-66 206
MS.gene03121.t1 MTR_4g006730 52.093 215 96 2 49 260 1 211 1.66e-66 206
MS.gene03121.t1 MTR_1g070385 43.192 213 120 1 38 250 64 275 2.86e-49 174
MS.gene03121.t1 MTR_4g006730 43.836 219 104 3 14 229 53 255 3.29e-47 158
MS.gene03121.t1 MTR_1g097840 52.047 171 75 2 32 199 70 236 7.67e-44 150
MS.gene03121.t1 MTR_7g010650 41.667 144 83 1 36 179 37 179 2.94e-35 125
MS.gene03121.t1 MTR_1g006490 39.336 211 118 7 35 238 18 225 3.93e-29 111
MS.gene03121.t1 MTR_8g013680 32.773 238 149 6 28 254 9 246 3.46e-28 108
MS.gene03121.t1 MTR_2g101370 33.061 245 152 6 19 254 6 247 2.03e-27 106
MS.gene03121.t1 MTR_6g069040 32.099 243 154 6 19 251 2 243 3.98e-27 105
MS.gene03121.t1 MTR_1g077540 31.818 242 156 4 19 251 2 243 5.39e-27 105
MS.gene03121.t1 MTR_4g006650 44.828 145 64 3 37 178 5 136 7.86e-25 101
MS.gene03121.t1 MTR_5g012810 34.802 227 137 6 22 238 3 228 4.04e-24 97.8
MS.gene03121.t1 MTR_1g006590 33.906 233 145 6 35 261 18 247 9.65e-24 96.7
MS.gene03121.t1 MTR_4g063090 33.632 223 139 5 41 255 26 247 1.09e-22 94.0
MS.gene03121.t1 MTR_7g103030 32.701 211 134 4 31 234 16 225 1.55e-21 90.9
MS.gene03121.t1 MTR_1g115405 32.068 237 147 6 35 261 20 252 2.22e-20 87.8
MS.gene03121.t1 MTR_2g094270 32.804 189 114 4 59 235 74 261 1.81e-17 80.5
MS.gene03121.t1 MTR_7g101190 29.741 232 142 6 25 235 28 259 1.14e-16 78.2
MS.gene03121.t1 MTR_7g023350 30.472 233 151 4 35 258 20 250 1.67e-16 77.4
MS.gene03121.t1 MTR_5g010150 31.019 216 127 6 39 235 55 267 2.25e-16 77.4
MS.gene03121.t1 MTR_5g082070 31.481 216 126 6 39 235 58 270 2.52e-16 77.4
MS.gene03121.t1 MTR_4g415300 31.604 212 130 6 39 235 59 270 2.59e-16 77.4
MS.gene03121.t1 MTR_3g070210 31.776 214 128 7 39 235 58 270 3.39e-16 77.0
MS.gene03121.t1 MTR_3g118010 29.224 219 140 4 34 237 35 253 4.65e-16 76.6
MS.gene03121.t1 MTR_8g098375 31.308 214 129 7 39 235 55 267 3.01e-15 74.3
MS.gene03121.t1 MTR_2g060360 30.622 209 137 4 35 236 18 225 7.84e-15 72.8
MS.gene03121.t1 MTR_2g094270 31.694 183 114 4 59 232 74 254 4.86e-14 70.5
MS.gene03121.t1 MTR_1g095070 28.571 182 114 4 78 245 24 203 2.94e-13 67.8
MS.gene03121.t1 MTR_3g070210 31.122 196 120 6 39 220 58 252 3.34e-13 68.6
MS.gene03121.t1 MTR_4g059390 30.337 178 112 3 70 235 82 259 5.56e-13 67.8
MS.gene03121.t1 MTR_4g075590 25.339 221 137 7 41 236 42 259 1.82e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene03121.t1 AT4G18910 69.880 249 68 1 22 263 38 286 2.88e-126 361
MS.gene03121.t1 AT4G19030 64.591 257 84 1 14 263 33 289 3.65e-121 348
MS.gene03121.t1 AT5G37820 59.426 244 96 1 20 263 30 270 8.18e-100 293
MS.gene03121.t1 AT5G37810 54.962 262 112 1 10 265 11 272 4.90e-99 291
MS.gene03121.t1 AT5G37820 59.426 244 96 1 20 263 66 306 4.98e-99 293
MS.gene03121.t1 AT1G31885 51.852 270 115 3 1 257 4 271 4.26e-95 283
MS.gene03121.t1 AT4G18910 71.351 185 46 1 22 199 38 222 9.96e-89 263
MS.gene03121.t1 AT1G31885 56.682 217 87 1 48 257 1 217 2.88e-86 258
MS.gene03121.t1 AT4G19030 67.568 185 53 1 22 199 41 225 1.45e-85 255
MS.gene03121.t1 AT2G34390 54.812 239 100 2 32 263 44 281 6.09e-85 256
MS.gene03121.t1 AT2G34390 54.545 231 97 2 37 260 7 236 5.26e-83 249
MS.gene03121.t1 AT4G19030 65.455 165 50 1 22 179 41 205 1.40e-73 224
MS.gene03121.t1 AT1G80760 50.204 245 117 2 8 250 52 293 5.84e-72 223
MS.gene03121.t1 AT4G10380 48.617 253 113 4 18 257 56 304 5.13e-69 216
MS.gene03121.t1 AT2G34390 58.286 175 65 2 32 199 44 217 1.04e-63 199
MS.gene03121.t1 AT2G34390 56.688 157 60 2 32 181 44 199 3.53e-55 177
MS.gene03121.t1 AT3G06100 42.202 218 124 2 35 251 45 261 8.39e-48 160
MS.gene03121.t1 AT3G06100 42.925 212 119 2 41 251 3 213 1.91e-47 158
MS.gene03121.t1 AT1G31885 50.000 118 51 2 1 112 4 119 2.00e-33 119
MS.gene03121.t1 AT3G16240 35.945 217 130 4 27 234 8 224 1.12e-30 115
MS.gene03121.t1 AT2G29870 58.621 87 36 0 151 237 20 106 5.72e-30 110
MS.gene03121.t1 AT5G47450 36.123 227 135 5 22 238 3 229 7.44e-29 110
MS.gene03121.t1 AT3G06100 39.437 142 84 2 112 252 41 181 6.58e-26 101
MS.gene03121.t1 AT4G17340 34.434 212 131 4 35 238 18 229 3.46e-25 100
MS.gene03121.t1 AT1G17810 32.271 251 153 6 22 258 13 260 5.73e-25 100
MS.gene03121.t1 AT1G73190 31.200 250 141 6 22 251 13 251 8.31e-24 97.4
MS.gene03121.t1 AT3G26520 33.884 242 139 8 31 258 17 251 2.86e-23 95.9
MS.gene03121.t1 AT2G36830 32.900 231 141 5 13 234 1 226 8.47e-23 94.4
MS.gene03121.t1 AT2G25810 36.493 211 124 7 35 238 18 225 2.81e-22 93.2
MS.gene03121.t1 AT4G01470 33.971 209 129 5 35 235 20 227 6.51e-20 86.7
MS.gene03121.t1 AT4G35100 30.242 248 147 6 16 237 7 254 2.05e-18 83.2
MS.gene03121.t1 AT4G35100 30.242 248 147 6 16 237 7 254 2.05e-18 83.2
MS.gene03121.t1 AT2G16850 30.137 219 138 5 34 237 34 252 6.92e-18 81.6
MS.gene03121.t1 AT5G60660 27.863 262 151 7 33 270 36 283 1.15e-17 81.3
MS.gene03121.t1 AT2G39010 31.771 192 119 3 66 245 77 268 3.97e-17 79.7
MS.gene03121.t1 AT3G47440 29.680 219 138 5 35 242 22 235 5.56e-17 78.6
MS.gene03121.t1 AT3G54820 27.273 253 163 5 14 245 16 268 6.55e-17 79.0
MS.gene03121.t1 AT3G53420 31.383 188 117 4 70 245 82 269 4.57e-16 76.6
MS.gene03121.t1 AT3G53420 31.383 188 117 4 70 245 82 269 4.57e-16 76.6
MS.gene03121.t1 AT1G01620 33.158 190 109 4 61 235 8 194 7.39e-16 74.7
MS.gene03121.t1 AT3G61430 30.986 213 130 6 39 235 55 266 9.29e-16 75.9
MS.gene03121.t1 AT3G61430 30.986 213 130 6 39 235 55 266 9.29e-16 75.9
MS.gene03121.t1 AT1G01620 31.163 215 127 5 39 235 55 266 1.31e-15 75.5
MS.gene03121.t1 AT4G00430 31.163 215 127 5 39 235 56 267 2.88e-15 74.3
MS.gene03121.t1 AT2G37170 29.255 188 121 3 70 245 80 267 5.24e-15 73.6
MS.gene03121.t1 AT4G23400 30.698 215 128 5 39 235 56 267 8.47e-15 73.2
MS.gene03121.t1 AT2G37170 29.255 188 121 3 70 245 123 310 8.81e-15 73.6
MS.gene03121.t1 AT2G45960 30.516 213 131 6 39 235 55 266 1.78e-14 72.0
MS.gene03121.t1 AT2G37180 29.843 191 116 4 70 245 80 267 2.30e-13 68.9
MS.gene03121.t1 AT2G45960 30.769 195 121 5 39 220 55 248 4.16e-12 65.1
MS.gene03121.t1 AT2G45960 30.769 195 121 5 39 220 55 248 5.66e-12 65.1

Find 77 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCTTATGTTCGTCATTTC+TGG 0.054064 2.3:-68565808 MS.gene03121:CDS
CTGGCTTATTTGTGATTCTA+AGG 0.227975 2.3:+68565138 None:intergenic
TCCTGGAATTGCAATTGTTT+GGG 0.271099 2.3:-68566641 MS.gene03121:CDS
TTCCTGGAATTGCAATTGTT+TGG 0.289298 2.3:-68566642 MS.gene03121:CDS
ACATTCTTCTTGATATTTGC+TGG 0.306945 2.3:-68566712 MS.gene03121:CDS
TCTTCATTCCTCAAAGGTTT+AGG 0.313855 2.3:-68565095 MS.gene03121:CDS
GTCACCTATTCTTGGAGCTT+TGG 0.322754 2.3:-68565183 MS.gene03121:CDS
GGGACTTGTTGTTATGGTAT+TGG 0.326696 2.3:-68566620 MS.gene03121:CDS
TATTCTATTGGTCATATATC+TGG 0.335738 2.3:-68566595 MS.gene03121:CDS
ATGGTATTGGTCTATTCTAT+TGG 0.341863 2.3:-68566607 MS.gene03121:CDS
GATAAAGTAGTGACACTTCC+TGG 0.350687 2.3:-68566658 MS.gene03121:CDS
CCCCAAACAATTGCAATTCC+AGG 0.359255 2.3:+68566640 None:intergenic
GCAGGCCCTAAGCTTCTTGC+TGG 0.362584 2.3:+68565248 None:intergenic
TTGTAATTTACCTGCTTCAA+TGG 0.368155 2.3:+68566520 None:intergenic
TCCATATTCCTCTATATTGA+TGG 0.376585 2.3:+68565216 None:intergenic
ATTGCTTCAGATTGGTGAAT+TGG 0.377943 2.3:-68565499 MS.gene03121:intron
TCAATTTGCAGGAACACTTC+CGG 0.378252 2.3:-68565878 MS.gene03121:CDS
CCATGCTTCCTAAACCTTTG+AGG 0.382124 2.3:+68565087 None:intergenic
ATGCTAAATGTGTTGTTTGC+GGG 0.383890 2.3:-68565450 MS.gene03121:CDS
TCACTACTTTATCATTGTTA+AGG 0.387999 2.3:+68566668 None:intergenic
GGCAAGGAAAATCAATTTGC+AGG 0.389055 2.3:-68565889 MS.gene03121:CDS
TATATGGTGTCACCTATTCT+TGG 0.391277 2.3:-68565191 MS.gene03121:CDS
CCTATTCTTGGAGCTTTGGC+TGG 0.403085 2.3:-68565179 MS.gene03121:CDS
TAGAGGAATATGGATATATA+TGG 0.403479 2.3:-68565207 MS.gene03121:CDS
AGGAACACTTCCGGCTGGTT+CGG 0.404188 2.3:-68565869 MS.gene03121:CDS
CTTCAGATTGGTGAATTGGC+TGG 0.406978 2.3:-68565495 MS.gene03121:intron
TTGGAGCTTTGGCTGGTACA+TGG 0.409617 2.3:-68565172 MS.gene03121:CDS
CTGCTTCAATGGAAACCTTC+CGG 0.415943 2.3:+68566531 None:intergenic
TGTTTGGGGACTTGTTGTTA+TGG 0.418172 2.3:-68566626 MS.gene03121:CDS
ATGAATCCAGCAAGAAGCTT+AGG 0.418884 2.3:-68565254 MS.gene03121:CDS
TTCTTGATATTTGCTGGATG+TGG 0.422703 2.3:-68566706 MS.gene03121:CDS
ATTCGACCACAAAAGCTTGA+TGG 0.429541 2.3:+68565845 None:intergenic
TGGAGCTTTGGCTGGTACAT+GGG 0.431169 2.3:-68565171 MS.gene03121:CDS
ACCATCAATATAGAGGAATA+TGG 0.435935 2.3:-68565217 MS.gene03121:CDS
GCTCTCAAACTCATATTTAG+TGG 0.443354 2.3:-68565910 MS.gene03121:CDS
TTTGAGGAATGAAGAACTCT+TGG 0.447025 2.3:+68565103 None:intergenic
CCAGCCAAAGCTCCAAGAAT+AGG 0.450629 2.3:+68565179 None:intergenic
CTCCAGAAATGACGAACATA+AGG 0.455985 2.3:+68565806 None:intergenic
TATATAATTGCTCAGGTCGC+TGG 0.462381 2.3:-68565952 MS.gene03121:CDS
ATTCATTGATGCTCCTGTGA+TGG 0.469127 2.3:+68565271 None:intergenic
TTCATTGATGCTCCTGTGAT+GGG 0.472526 2.3:+68565272 None:intergenic
CCTCAAAGGTTTAGGAAGCA+TGG 0.503782 2.3:-68565087 MS.gene03121:CDS
CGGAAGGTTTCCATTGAAGC+AGG 0.504912 2.3:-68566530 MS.gene03121:intron
TATTGATGGTGCACAATAGC+AGG 0.505141 2.3:+68565230 None:intergenic
TGAATCCAGCAAGAAGCTTA+GGG 0.507995 2.3:-68565253 MS.gene03121:CDS
CCTGGAATTGCAATTGTTTG+GGG 0.511430 2.3:-68566640 MS.gene03121:CDS
GATTGTTGAATATAGTTGGT+AGG 0.511991 2.3:-68566748 MS.gene03121:intron
CATGCTAAATGTGTTGTTTG+CGG 0.516473 2.3:-68565451 MS.gene03121:CDS
GAATTGGCTGGACTTGCAGT+TGG 0.518324 2.3:-68565483 MS.gene03121:CDS
CTCAAAGGTTTAGGAAGCAT+GGG 0.520761 2.3:-68565086 MS.gene03121:CDS
CTCTTGGTGAGCTCACGTAC+TGG 0.527633 2.3:+68565119 None:intergenic
ACCCGCTTATATAATTGCTC+AGG 0.527901 2.3:-68565959 MS.gene03121:CDS
AAGAGGAACATGATCATCAA+TGG 0.530972 2.3:+68566860 None:intergenic
ACCTGAGCAATTATATAAGC+GGG 0.540434 2.3:+68565958 None:intergenic
GTGTGAGCAATGGTGACAGC+GGG 0.558512 2.3:+68566559 None:intergenic
AACATGGGTGATATTAGCAA+TGG 0.558983 2.3:-68566942 MS.gene03121:CDS
ACCATTGCTCACACTACCAC+CGG 0.562160 2.3:-68566550 MS.gene03121:CDS
TGCTGGATGTGGTTCTGTGG+TGG 0.564205 2.3:-68566695 MS.gene03121:CDS
AAGAGTTCTTCATTCCTCAA+AGG 0.566116 2.3:-68565101 MS.gene03121:CDS
GCTTGATGGTCCGAACCAGC+CGG 0.573042 2.3:+68565859 None:intergenic
TGCTAAATGTGTTGTTTGCG+GGG 0.584865 2.3:-68565449 MS.gene03121:intron
TTTGCAGGAACACTTCCGGC+TGG 0.585819 2.3:-68565874 MS.gene03121:CDS
ATAGTTGGTAGGAGAAGTTG+TGG 0.585849 2.3:-68566737 MS.gene03121:intron
CAAACTCATATTTAGTGGCA+AGG 0.586123 2.3:-68565905 MS.gene03121:CDS
ATTGTGCACCATCAATATAG+AGG 0.592044 2.3:-68565224 MS.gene03121:CDS
ATTTGCTGGATGTGGTTCTG+TGG 0.593346 2.3:-68566698 MS.gene03121:CDS
AATTACCGCTCTATTATCAG+TGG 0.623253 2.3:+68565780 None:intergenic
ATATTTGACAGACCCATCAC+AGG 0.629986 2.3:-68565284 MS.gene03121:intron
GACCTGAGCAATTATATAAG+CGG 0.630532 2.3:+68565957 None:intergenic
TAGCAATGGAAATCTTGATG+TGG 0.656350 2.3:-68566928 MS.gene03121:CDS
TTGCTCACACTACCACCGGA+AGG 0.656735 2.3:-68566546 MS.gene03121:CDS
TCCGGTGGTAGTGTGAGCAA+TGG 0.668876 2.3:+68566549 None:intergenic
TAGTTGGTAGGAGAAGTTGT+GGG 0.670961 2.3:-68566736 MS.gene03121:CDS
CTTCAATGGAAACCTTCCGG+TGG 0.695539 2.3:+68566534 None:intergenic
AGTGTGAGCAATGGTGACAG+CGG 0.739883 2.3:+68566558 None:intergenic
AGTTGCCACTGATAATAGAG+CGG 0.742875 2.3:-68565785 MS.gene03121:intron
GTGGTTATGAATGTAAACGA+TGG 0.756470 2.3:-68566909 MS.gene03121:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAAAAATTAATAATATTT+TGG + chr2.3:68566018-68566037 None:intergenic 0.0%
!! TAGATAAAAATGTATAATAT+TGG + chr2.3:68566644-68566663 None:intergenic 10.0%
!!! ATTATACATTTTTATCTAAT+AGG - chr2.3:68566646-68566665 MS.gene03121:CDS 10.0%
!!! ATTTTTTTTTTTTAAATGCT+TGG - chr2.3:68566255-68566274 MS.gene03121:intron 10.0%
!!! TTATACATTTTTATCTAATA+GGG - chr2.3:68566647-68566666 MS.gene03121:CDS 10.0%
!!! TTTATTTAATTTTTTCTTGT+AGG - chr2.3:68566032-68566051 MS.gene03121:intron 10.0%
!! AAATGAATAATATTAGACAA+AGG + chr2.3:68566491-68566510 None:intergenic 15.0%
!! TTATGATATAATATTTGGTA+AGG + chr2.3:68566606-68566625 None:intergenic 15.0%
!!! GCATTTTATGATATAATATT+TGG + chr2.3:68566611-68566630 None:intergenic 15.0%
!!! TATTGATTGTTGAATATAGT+TGG - chr2.3:68565263-68565282 MS.gene03121:CDS 20.0%
!!! TTTTATTTATTGCTTCAGAT+TGG - chr2.3:68566508-68566527 MS.gene03121:intron 20.0%
! AGATCTTCTGACTAAAAAAA+TGG - chr2.3:68565239-68565258 MS.gene03121:CDS 25.0%
! TAGAGGAATATGGATATATA+TGG - chr2.3:68566808-68566827 MS.gene03121:intron 25.0%
! TATTCTATTGGTCATATATC+TGG - chr2.3:68565420-68565439 MS.gene03121:intron 25.0%
! TCACTACTTTATCATTGTTA+AGG + chr2.3:68565350-68565369 None:intergenic 25.0%
! TCTCTCTTAAGTTATTTGAT+CGG - chr2.3:68565558-68565577 MS.gene03121:intron 25.0%
!!! AATATTTTGGACTGCAAAAA+TGG + chr2.3:68566005-68566024 None:intergenic 25.0%
!!! AGCTAAGAGTTTAAAGTTTT+GGG - chr2.3:68565675-68565694 MS.gene03121:intron 25.0%
ACATTCTTCTTGATATTTGC+TGG - chr2.3:68565303-68565322 MS.gene03121:intron 30.0%
ACCATCAATATAGAGGAATA+TGG - chr2.3:68566798-68566817 MS.gene03121:intron 30.0%
GATTGTTGAATATAGTTGGT+AGG - chr2.3:68565267-68565286 MS.gene03121:CDS 30.0%
TAAATGCTTAGAGTAACAGT+TGG - chr2.3:68565741-68565760 MS.gene03121:intron 30.0%
TATTTCCTTCTTCTTCACAT+AGG + chr2.3:68565929-68565948 None:intergenic 30.0%
TCCATATTCCTCTATATTGA+TGG + chr2.3:68566802-68566821 None:intergenic 30.0%
TTAAGTTATTTGATCGGAGA+TGG - chr2.3:68565564-68565583 MS.gene03121:intron 30.0%
TTGTAATTTACCTGCTTCAA+TGG + chr2.3:68565498-68565517 None:intergenic 30.0%
! AAAATCACCTTTTGCAAAAG+AGG + chr2.3:68565175-68565194 None:intergenic 30.0%
!! ATGGTATTGGTCTATTCTAT+TGG - chr2.3:68565408-68565427 MS.gene03121:intron 30.0%
!! GAGCTAAGAGTTTAAAGTTT+TGG - chr2.3:68565674-68565693 MS.gene03121:intron 30.0%
!! GCTAAGAGTTTAAAGTTTTG+GGG - chr2.3:68565676-68565695 MS.gene03121:intron 30.0%
!!! GTTACATGTTTTAGTGAGAA+AGG - chr2.3:68566677-68566696 MS.gene03121:CDS 30.0%
AAGAGGAACATGATCATCAA+TGG + chr2.3:68565158-68565177 None:intergenic 35.0%
AAGAGTTCTTCATTCCTCAA+AGG - chr2.3:68566914-68566933 MS.gene03121:CDS 35.0%
AATTACCGCTCTATTATCAG+TGG + chr2.3:68566238-68566257 None:intergenic 35.0%
ACCTGAGCAATTATATAAGC+GGG + chr2.3:68566060-68566079 None:intergenic 35.0%
AGAAGAAGGAAATAGTTGGT+AGG - chr2.3:68565935-68565954 MS.gene03121:CDS 35.0%
ATAATTATAGAATGGGCCTC+AGG + chr2.3:68566453-68566472 None:intergenic 35.0%
ATTGCTTCAGATTGGTGAAT+TGG - chr2.3:68566516-68566535 MS.gene03121:intron 35.0%
ATTGTGCACCATCAATATAG+AGG - chr2.3:68566791-68566810 MS.gene03121:intron 35.0%
CAAACTCATATTTAGTGGCA+AGG - chr2.3:68566110-68566129 MS.gene03121:intron 35.0%
CCAACTAATGCTTCAAACAT+AGG + chr2.3:68565973-68565992 None:intergenic 35.0%
CTGGCTTATTTGTGATTCTA+AGG + chr2.3:68566880-68566899 None:intergenic 35.0%
GACCTGAGCAATTATATAAG+CGG + chr2.3:68566061-68566080 None:intergenic 35.0%
GCTCGATGTAGTAACAAAAA+GGG - chr2.3:68565869-68565888 MS.gene03121:CDS 35.0%
GCTCTCAAACTCATATTTAG+TGG - chr2.3:68566105-68566124 MS.gene03121:intron 35.0%
GTGAAGAAGAAGGAAATAGT+TGG - chr2.3:68565931-68565950 MS.gene03121:CDS 35.0%
GTGGTTATGAATGTAAACGA+TGG - chr2.3:68565106-68565125 MS.gene03121:CDS 35.0%
TACCTTATGTTCGTCATTTC+TGG - chr2.3:68566207-68566226 MS.gene03121:intron 35.0%
TAGCAATGGAAATCTTGATG+TGG - chr2.3:68565087-68565106 MS.gene03121:CDS 35.0%
TCAAACATAGGCCCAAATAA+CGG + chr2.3:68565961-68565980 None:intergenic 35.0%
TCCTGGAATTGCAATTGTTT+GGG - chr2.3:68565374-68565393 MS.gene03121:intron 35.0%
TCTTCATTCCTCAAAGGTTT+AGG - chr2.3:68566920-68566939 MS.gene03121:CDS 35.0%
TGCTCGATGTAGTAACAAAA+AGG - chr2.3:68565868-68565887 MS.gene03121:CDS 35.0%
TTACCTTTAAATCAGTCCTG+AGG - chr2.3:68566434-68566453 MS.gene03121:intron 35.0%
TTCAACCTATGTGAAGAAGA+AGG - chr2.3:68565921-68565940 MS.gene03121:CDS 35.0%
TTCCTGGAATTGCAATTGTT+TGG - chr2.3:68565373-68565392 MS.gene03121:intron 35.0%
TTTGAGGAATGAAGAACTCT+TGG + chr2.3:68566915-68566934 None:intergenic 35.0%
! AATAAAGGCGCAATGTTACA+AGG + chr2.3:68565807-68565826 None:intergenic 35.0%
! ACATAGGTTGAAACTTGCTT+TGG + chr2.3:68565913-68565932 None:intergenic 35.0%
! CCTATGTTTGAAGCATTAGT+TGG - chr2.3:68565970-68565989 MS.gene03121:intron 35.0%
! TCATGTTCCTCTTTTGCAAA+AGG - chr2.3:68565165-68565184 MS.gene03121:CDS 35.0%
! TTCTTGATATTTGCTGGATG+TGG - chr2.3:68565309-68565328 MS.gene03121:intron 35.0%
!! ATGCTAAATGTGTTGTTTGC+GGG - chr2.3:68566565-68566584 MS.gene03121:CDS 35.0%
!! CATGCTAAATGTGTTGTTTG+CGG - chr2.3:68566564-68566583 MS.gene03121:CDS 35.0%
!! TATATGGTGTCACCTATTCT+TGG - chr2.3:68566824-68566843 MS.gene03121:intron 35.0%
AAAGGGTGGTGTGAAAAGTA+TGG - chr2.3:68565886-68565905 MS.gene03121:CDS 40.0%
ACCCGCTTATATAATTGCTC+AGG - chr2.3:68566056-68566075 MS.gene03121:intron 40.0%
AGTTGCCACTGATAATAGAG+CGG - chr2.3:68566230-68566249 MS.gene03121:intron 40.0%
ATATTTGACAGACCCATCAC+AGG - chr2.3:68566731-68566750 MS.gene03121:CDS 40.0%
ATGAATCCAGCAAGAAGCTT+AGG - chr2.3:68566761-68566780 MS.gene03121:intron 40.0%
ATTCATTGATGCTCCTGTGA+TGG + chr2.3:68566747-68566766 None:intergenic 40.0%
ATTCGACCACAAAAGCTTGA+TGG + chr2.3:68566173-68566192 None:intergenic 40.0%
CCTGGAATTGCAATTGTTTG+GGG - chr2.3:68565375-68565394 MS.gene03121:intron 40.0%
CGATGTAGTAACAAAAAGGG+TGG - chr2.3:68565872-68565891 MS.gene03121:CDS 40.0%
CTCCAGAAATGACGAACATA+AGG + chr2.3:68566212-68566231 None:intergenic 40.0%
GAGATTGTAGGTGGTTGAAA+AGG - chr2.3:68565607-68565626 MS.gene03121:intron 40.0%
GATAAAGTAGTGACACTTCC+TGG - chr2.3:68565357-68565376 MS.gene03121:intron 40.0%
GGCAAGGAAAATCAATTTGC+AGG - chr2.3:68566126-68566145 MS.gene03121:intron 40.0%
TATATAATTGCTCAGGTCGC+TGG - chr2.3:68566063-68566082 MS.gene03121:intron 40.0%
TCAATTTGCAGGAACACTTC+CGG - chr2.3:68566137-68566156 MS.gene03121:intron 40.0%
TGAATCCAGCAAGAAGCTTA+GGG - chr2.3:68566762-68566781 MS.gene03121:intron 40.0%
TTCATTGATGCTCCTGTGAT+GGG + chr2.3:68566746-68566765 None:intergenic 40.0%
! CTCAAAGGTTTAGGAAGCAT+GGG - chr2.3:68566929-68566948 MS.gene03121:CDS 40.0%
!! ATAGTTGGTAGGAGAAGTTG+TGG - chr2.3:68565278-68565297 MS.gene03121:intron 40.0%
!! ATAGTTGGTAGGCCGTTATT+TGG - chr2.3:68565946-68565965 MS.gene03121:CDS 40.0%
!! GGGACTTGTTGTTATGGTAT+TGG - chr2.3:68565395-68565414 MS.gene03121:intron 40.0%
!! TAGTTGGTAGGAGAAGTTGT+GGG - chr2.3:68565279-68565298 MS.gene03121:intron 40.0%
!! TAGTTGGTAGGCCGTTATTT+GGG - chr2.3:68565947-68565966 MS.gene03121:CDS 40.0%
!! TATTGATGGTGCACAATAGC+AGG + chr2.3:68566788-68566807 None:intergenic 40.0%
!! TGCTAAATGTGTTGTTTGCG+GGG - chr2.3:68566566-68566585 MS.gene03121:CDS 40.0%
!! TGTTTGGGGACTTGTTGTTA+TGG - chr2.3:68565389-68565408 MS.gene03121:intron 40.0%
AAGAACGTACCTAGGAGGAT+AGG - chr2.3:68565703-68565722 MS.gene03121:intron 45.0%
ATCGGAGATGGTCAGAAGAA+CGG - chr2.3:68565576-68565595 MS.gene03121:intron 45.0%
CCATGCTTCCTAAACCTTTG+AGG + chr2.3:68566931-68566950 None:intergenic 45.0%
CCCCAAACAATTGCAATTCC+AGG + chr2.3:68565378-68565397 None:intergenic 45.0%
CCTCAAAGGTTTAGGAAGCA+TGG - chr2.3:68566928-68566947 MS.gene03121:CDS 45.0%
CTGCTTCAATGGAAACCTTC+CGG + chr2.3:68565487-68565506 None:intergenic 45.0%
CTTCAGATTGGTGAATTGGC+TGG - chr2.3:68566520-68566539 MS.gene03121:intron 45.0%
GGTACCTGCAAAAGACACAT+TGG - chr2.3:68565634-68565653 MS.gene03121:intron 45.0%
GTCACCTATTCTTGGAGCTT+TGG - chr2.3:68566832-68566851 MS.gene03121:intron 45.0%
GTTAGAAGAACGTACCTAGG+AGG - chr2.3:68565698-68565717 MS.gene03121:intron 45.0%
TCAACCACCTACAATCTCGT+CGG + chr2.3:68565605-68565624 None:intergenic 45.0%
! AACGCCAATGTGTCTTTTGC+AGG + chr2.3:68565641-68565660 None:intergenic 45.0%
! ATTTGCTGGATGTGGTTCTG+TGG - chr2.3:68565317-68565336 MS.gene03121:intron 45.0%
! GTTTGAAGCATTAGTTGGCG+AGG - chr2.3:68565975-68565994 MS.gene03121:intron 45.0%
! TTCGGACCATCAAGCTTTTG+TGG - chr2.3:68566164-68566183 MS.gene03121:intron 45.0%
!! AGTAACAGTTGGTGAGCTGT+TGG - chr2.3:68565752-68565771 MS.gene03121:intron 45.0%
!! ATATTTAAATAATTATAGAA+TGG + chr2.3:68566461-68566480 None:intergenic 5.0%
!! TATTTAAATAATTATAGAAT+GGG + chr2.3:68566460-68566479 None:intergenic 5.0%
ACCATTGCTCACACTACCAC+CGG - chr2.3:68565465-68565484 MS.gene03121:CDS 50.0%
ACGGTATCCGACGAGATTGT+AGG - chr2.3:68565595-68565614 MS.gene03121:intron 50.0%
AGAGCTTCTCCTATCCTCCT+AGG + chr2.3:68565715-68565734 None:intergenic 50.0%
AGTGTGAGCAATGGTGACAG+CGG + chr2.3:68565460-68565479 None:intergenic 50.0%
CCAGCCAAAGCTCCAAGAAT+AGG + chr2.3:68566839-68566858 None:intergenic 50.0%
CGGAAGGTTTCCATTGAAGC+AGG - chr2.3:68565485-68565504 MS.gene03121:CDS 50.0%
CTTCAATGGAAACCTTCCGG+TGG + chr2.3:68565484-68565503 None:intergenic 50.0%
GAATTGGCTGGACTTGCAGT+TGG - chr2.3:68566532-68566551 MS.gene03121:CDS 50.0%
GGGGTTAGAAGAACGTACCT+AGG - chr2.3:68565695-68565714 MS.gene03121:intron 50.0%
GTAGGTGGTTGAAAAGGCAG+AGG - chr2.3:68565613-68565632 MS.gene03121:intron 50.0%
GTATCCGACGAGATTGTAGG+TGG - chr2.3:68565598-68565617 MS.gene03121:intron 50.0%
TGTTGGTCGACGGTTACAGA+TGG - chr2.3:68565769-68565788 MS.gene03121:intron 50.0%
TTGCGCCTTTATTGCACGAG+AGG - chr2.3:68565814-68565833 MS.gene03121:CDS 50.0%
! CCTATTCTTGGAGCTTTGGC+TGG - chr2.3:68566836-68566855 MS.gene03121:intron 50.0%
! GGGCCTCAGGACTGATTTAA+AGG + chr2.3:68566440-68566459 None:intergenic 50.0%
! TGGAGCTTTGGCTGGTACAT+GGG - chr2.3:68566844-68566863 MS.gene03121:intron 50.0%
! TTGGAGCTTTGGCTGGTACA+TGG - chr2.3:68566843-68566862 MS.gene03121:intron 50.0%
CGCCTCCTCTCGTGCAATAA+AGG + chr2.3:68565822-68565841 None:intergenic 55.0%
CGCCTTTATTGCACGAGAGG+AGG - chr2.3:68565817-68565836 MS.gene03121:CDS 55.0%
CTCTTGGTGAGCTCACGTAC+TGG + chr2.3:68566899-68566918 None:intergenic 55.0%
GTGTGAGCAATGGTGACAGC+GGG + chr2.3:68565459-68565478 None:intergenic 55.0%
TGGTCGACGGTTACAGATGG+CGG - chr2.3:68565772-68565791 MS.gene03121:intron 55.0%
TTGCTCACACTACCACCGGA+AGG - chr2.3:68565469-68565488 MS.gene03121:CDS 55.0%
TTTGCAGGAACACTTCCGGC+TGG - chr2.3:68566141-68566160 MS.gene03121:intron 55.0%
! AGGAACACTTCCGGCTGGTT+CGG - chr2.3:68566146-68566165 MS.gene03121:intron 55.0%
! GTTGGTGAGCTGTTGGTCGA+CGG - chr2.3:68565759-68565778 MS.gene03121:intron 55.0%
! TCCGGTGGTAGTGTGAGCAA+TGG + chr2.3:68565469-68565488 None:intergenic 55.0%
! TGCTGGATGTGGTTCTGTGG+TGG - chr2.3:68565320-68565339 MS.gene03121:intron 55.0%
!! AGAGGAGGCGTTACAGACTC+AGG - chr2.3:68565832-68565851 MS.gene03121:CDS 55.0%
GCAGGCCCTAAGCTTCTTGC+TGG + chr2.3:68566770-68566789 None:intergenic 60.0%
! GCTTGATGGTCCGAACCAGC+CGG + chr2.3:68566159-68566178 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 68565076 68566961 68565076 ID=MS.gene03121
chr2.3 mRNA 68565076 68566961 68565076 ID=MS.gene03121.t1;Parent=MS.gene03121
chr2.3 exon 68566851 68566961 68566851 ID=MS.gene03121.t1.exon1;Parent=MS.gene03121.t1
chr2.3 CDS 68566851 68566961 68566851 ID=cds.MS.gene03121.t1;Parent=MS.gene03121.t1
chr2.3 exon 68566531 68566755 68566531 ID=MS.gene03121.t1.exon2;Parent=MS.gene03121.t1
chr2.3 CDS 68566531 68566755 68566531 ID=cds.MS.gene03121.t1;Parent=MS.gene03121.t1
chr2.3 exon 68565786 68565983 68565786 ID=MS.gene03121.t1.exon3;Parent=MS.gene03121.t1
chr2.3 CDS 68565786 68565983 68565786 ID=cds.MS.gene03121.t1;Parent=MS.gene03121.t1
chr2.3 exon 68565450 68565511 68565450 ID=MS.gene03121.t1.exon4;Parent=MS.gene03121.t1
chr2.3 CDS 68565450 68565511 68565450 ID=cds.MS.gene03121.t1;Parent=MS.gene03121.t1
chr2.3 exon 68565076 68565295 68565076 ID=MS.gene03121.t1.exon5;Parent=MS.gene03121.t1
chr2.3 CDS 68565076 68565295 68565076 ID=cds.MS.gene03121.t1;Parent=MS.gene03121.t1
Gene Sequence

>MS.gene03121

ATGGGTGATATTAGCAATGGAAATCTTGATGTGGTTATGAATGTAAACGATGGTGCAACAAAAAAATGTGACGACACAACCATTGATGATCATGTTCCTCTTTTGCAAAAGGTGATTTTCTTTTTCATTCAAAAAATTAATTGTTGTTTATGATTAATTAATTAGATCTTCTGACTAAAAAAATGGATATTGATTGTTGAATATAGTTGGTAGGAGAAGTTGTGGGAACATTCTTCTTGATATTTGCTGGATGTGGTTCTGTGGTGGTGAACCTTAACAATGATAAAGTAGTGACACTTCCTGGAATTGCAATTGTTTGGGGACTTGTTGTTATGGTATTGGTCTATTCTATTGGTCATATATCTGGTGCTCATTTCAATCCCGCTGTCACCATTGCTCACACTACCACCGGAAGGTTTCCATTGAAGCAGGTAAATTACAAAACACAAGAACAATAATTCATATTAAGATAAACATAATTTTCTCTCTTAAGTTATTTGATCGGAGATGGTCAGAAGAACGGTATCCGACGAGATTGTAGGTGGTTGAAAAGGCAGAGGTACCTGCAAAAGACACATTGGCGTTCAAGTCAGCATGAGAGCTAAGAGTTTAAAGTTTTGGGGTTAGAAGAACGTACCTAGGAGGATAGGAGAAGCTCTCTTATATAAATGCTTAGAGTAACAGTTGGTGAGCTGTTGGTCGACGGTTACAGATGGCGGTGTTTGTAACCTTGTAACATTGCGCCTTTATTGCACGAGAGGAGGCGTTACAGACTCAGGTGAGATGAACGTTTGCTCGATGTAGTAACAAAAAGGGTGGTGTGAAAAGTATGGACCAAAGCAAGTTTCAACCTATGTGAAGAAGAAGGAAATAGTTGGTAGGCCGTTATTTGGGCCTATGTTTGAAGCATTAGTTGGCGAGGACAACCATTTTTGCAGTCCAAAATATTATTAATTTTTATTTAATTTTTTCTTGTAGGTACCCGCTTATATAATTGCTCAGGTCGCTGGCTCAACACTTGCAAGTGAAGCTCTCAAACTCATATTTAGTGGCAAGGAAAATCAATTTGCAGGAACACTTCCGGCTGGTTCGGACCATCAAGCTTTTGTGGTCGAATTTATTATCACTTTCTACCTTATGTTCGTCATTTCTGGAGTTGCCACTGATAATAGAGCGGTAATTTTTTTTTTTTAAATGCTTGGTTGATAATAGTAATTGATAATTATATTTCTATTGATCATTTTGATTAAGATAAAATTGTGACTCAAGTTAATTGACTATTTCTTTCAACAACAATTAAAAGCTCATTGACTAGATGTATAAACATATTTATAATGTTAAAATCACTTCAATAAATGTTACCTTTAAATCAGTCCTGAGGCCCATTCTATAATTATTTAAATATCATTTAACCTTTGTCTAATATTATTCATTTTATTTATTGCTTCAGATTGGTGAATTGGCTGGACTTGCAGTTGGATCTACAGTCATGCTAAATGTGTTGTTTGCGGGGTATAGATCTCAAAACCTTACCAAATATTATATCATAAAATGCATGCTTTGCACCAATATTATACATTTTTATCTAATAGGGTTAATATGTTACATGTTTTAGTGAGAAAGGTTCAAATTAAATCTTACTTTTGAGTGAAAATATATTTGACAGACCCATCACAGGAGCATCAATGAATCCAGCAAGAAGCTTAGGGCCTGCTATTGTGCACCATCAATATAGAGGAATATGGATATATATGGTGTCACCTATTCTTGGAGCTTTGGCTGGTACATGGGCATACACTTTCCTTAGAATCACAAATAAGCCAGTACGTGAGCTCACCAAGAGTTCTTCATTCCTCAAAGGTTTAGGAAGCATGGGAGCTGAGTGA
Protein sequence

>MS.gene03121.t1

MGDISNGNLDVVMNVNDGATKKCDDTTIDDHVPLLQKLVGEVVGTFFLIFAGCGSVVVNLNNDKVVTLPGIAIVWGLVVMVLVYSIGHISGAHFNPAVTIAHTTTGRFPLKQVPAYIIAQVAGSTLASEALKLIFSGKENQFAGTLPAGSDHQAFVVEFIITFYLMFVISGVATDNRAIGELAGLAVGSTVMLNVLFAGPITGASMNPARSLGPAIVHHQYRGIWIYMVSPILGALAGTWAYTFLRITNKPVRELTKSSSFLKGLGSMGAE