AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004321

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004321.t1 MTR_2g017570 97.802 273 6 0 1 273 1 273 0.0 547
MS.gene004321.t1 MTR_2g017610 97.070 273 8 0 1 273 1 273 0.0 541
MS.gene004321.t1 MTR_2g017590 81.061 264 50 0 7 270 5 268 5.24e-163 453
MS.gene004321.t1 MTR_2g017620 78.195 266 58 0 1 266 1 266 3.28e-154 431
MS.gene004321.t1 MTR_8g087720 76.493 268 63 0 1 268 1 268 1.02e-152 427
MS.gene004321.t1 MTR_2g017620 79.365 252 52 0 15 266 1 252 2.19e-148 416
MS.gene004321.t1 MTR_8g087710 70.480 271 80 0 3 273 4 274 2.70e-142 401
MS.gene004321.t1 MTR_2g017660 69.853 272 75 2 1 265 1 272 3.39e-141 398
MS.gene004321.t1 MTR_2g017620 82.703 185 32 0 82 266 1 185 7.93e-111 318
MS.gene004321.t1 MTR_8g087720 77.612 201 45 0 1 201 1 201 8.87e-111 318
MS.gene004321.t1 MTR_4g084880 58.025 243 100 1 32 272 29 271 1.62e-102 300
MS.gene004321.t1 MTR_8g087720 74.457 184 47 0 1 184 1 184 3.83e-96 281
MS.gene004321.t1 MTR_5g079570 49.612 258 121 3 1 253 1 254 1.01e-84 254
MS.gene004321.t1 MTR_3g008920 53.556 239 108 2 18 256 8 243 6.43e-84 252
MS.gene004321.t1 MTR_4g006730 50.840 238 108 3 34 265 71 305 2.49e-70 219
MS.gene004321.t1 MTR_7g010650 47.085 223 115 2 31 251 28 249 8.49e-69 214
MS.gene004321.t1 MTR_2g104940 51.163 215 100 2 36 248 78 289 1.63e-67 212
MS.gene004321.t1 MTR_5g063930 45.522 268 129 7 1 260 1 259 6.05e-67 209
MS.gene004321.t1 MTR_1g097840 46.800 250 114 4 26 259 54 300 3.26e-66 208
MS.gene004321.t1 MTR_4g006630 50.226 221 101 3 51 265 1 218 4.30e-64 200
MS.gene004321.t1 MTR_4g006730 50.226 221 101 3 51 265 1 218 6.57e-64 199
MS.gene004321.t1 MTR_4g006730 52.941 170 75 2 34 201 71 237 5.27e-47 158
MS.gene004321.t1 MTR_1g070385 39.906 213 127 1 40 252 64 275 2.07e-44 160
MS.gene004321.t1 MTR_1g097840 47.849 186 84 3 26 201 54 236 1.39e-43 149
MS.gene004321.t1 MTR_7g010650 43.137 153 84 2 31 181 28 179 1.71e-37 131
MS.gene004321.t1 MTR_4g006650 47.222 144 62 3 39 180 5 136 7.14e-27 107
MS.gene004321.t1 MTR_8g013680 32.850 207 132 3 37 236 18 224 1.52e-25 101
MS.gene004321.t1 MTR_1g077540 32.218 239 144 6 26 253 12 243 5.52e-24 97.8
MS.gene004321.t1 MTR_2g101370 30.568 229 150 5 37 256 19 247 9.47e-23 94.4
MS.gene004321.t1 MTR_5g012810 33.913 230 141 7 37 256 18 246 1.56e-22 93.6
MS.gene004321.t1 MTR_1g006490 32.143 224 136 7 19 236 7 220 5.42e-22 92.0
MS.gene004321.t1 MTR_6g069040 29.583 240 153 6 24 253 10 243 7.96e-22 92.0
MS.gene004321.t1 MTR_1g006590 32.000 250 150 9 19 258 7 246 3.70e-21 90.1
MS.gene004321.t1 MTR_4g063090 32.159 227 140 7 43 256 26 251 2.69e-17 79.3
MS.gene004321.t1 MTR_7g103030 30.667 225 146 6 37 252 20 243 3.83e-17 79.0
MS.gene004321.t1 MTR_3g118010 28.384 229 141 5 38 247 37 261 1.72e-16 77.8
MS.gene004321.t1 MTR_2g094270 30.899 178 111 3 72 237 84 261 2.09e-16 77.8
MS.gene004321.t1 MTR_5g082070 31.019 216 127 7 41 237 58 270 3.79e-16 77.0
MS.gene004321.t1 MTR_7g101190 29.412 221 135 6 38 237 39 259 1.28e-15 75.5
MS.gene004321.t1 MTR_5g010150 30.047 213 133 5 41 237 55 267 1.36e-15 75.5
MS.gene004321.t1 MTR_1g115405 32.227 211 130 5 37 237 20 227 1.61e-15 74.7
MS.gene004321.t1 MTR_8g098375 30.516 213 132 6 41 237 55 267 1.74e-15 75.1
MS.gene004321.t1 MTR_3g070210 28.638 213 136 5 41 237 58 270 1.14e-14 72.8
MS.gene004321.t1 MTR_4g415300 29.245 212 135 5 41 237 59 270 2.24e-14 72.0
MS.gene004321.t1 MTR_2g094270 30.573 157 100 2 72 219 84 240 7.05e-14 70.1
MS.gene004321.t1 MTR_3g070210 28.205 195 127 4 41 222 58 252 2.62e-13 68.6
MS.gene004321.t1 MTR_1g095070 28.571 182 112 4 81 247 25 203 3.11e-13 67.8
MS.gene004321.t1 MTR_2g060360 26.639 244 164 5 37 273 18 253 9.36e-13 67.0
MS.gene004321.t1 MTR_7g023350 30.263 228 135 8 37 251 20 236 9.43e-13 66.6
MS.gene004321.t1 MTR_4g059390 29.282 181 110 5 72 237 82 259 7.72e-12 64.7
MS.gene004321.t1 MTR_1g095070 28.571 154 95 3 81 222 25 175 4.15e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004321.t1 AT4G18910 70.980 255 67 1 26 273 40 294 3.60e-131 373
MS.gene004321.t1 AT4G19030 68.651 252 72 1 26 270 43 294 4.31e-125 358
MS.gene004321.t1 AT5G37820 58.431 255 103 1 18 269 20 274 7.56e-100 293
MS.gene004321.t1 AT5G37820 58.431 255 103 1 18 269 56 310 2.88e-99 293
MS.gene004321.t1 AT5G37810 54.015 274 119 3 1 268 1 273 1.36e-98 290
MS.gene004321.t1 AT4G18910 75.410 183 38 1 26 201 40 222 5.79e-92 271
MS.gene004321.t1 AT1G31885 53.036 247 109 1 20 259 25 271 2.96e-90 270
MS.gene004321.t1 AT2G34390 51.748 286 120 3 1 269 1 285 3.12e-90 269
MS.gene004321.t1 AT4G19030 70.330 182 47 1 26 200 43 224 1.74e-87 260
MS.gene004321.t1 AT2G34390 55.462 238 98 2 39 269 7 243 4.78e-86 257
MS.gene004321.t1 AT1G31885 54.839 217 91 1 50 259 1 217 1.57e-83 251
MS.gene004321.t1 AT4G19030 68.519 162 44 1 27 181 44 205 2.35e-75 229
MS.gene004321.t1 AT2G34390 54.128 218 82 3 1 201 1 217 1.31e-68 212
MS.gene004321.t1 AT4G10380 47.755 245 113 4 27 259 63 304 1.46e-67 212
MS.gene004321.t1 AT1G80760 52.036 221 101 2 34 252 76 293 1.73e-67 212
MS.gene004321.t1 AT2G34390 52.500 200 77 3 1 183 1 199 1.59e-59 189
MS.gene004321.t1 AT3G06100 40.367 218 128 2 37 253 45 261 2.09e-44 151
MS.gene004321.t1 AT3G06100 40.654 214 125 2 41 253 1 213 5.01e-44 149
MS.gene004321.t1 AT1G31885 56.842 95 41 0 20 114 25 119 2.28e-31 114
MS.gene004321.t1 AT2G29870 48.718 117 60 0 153 269 20 136 1.47e-30 111
MS.gene004321.t1 AT3G16240 34.783 207 128 3 37 236 18 224 3.32e-28 108
MS.gene004321.t1 AT5G47450 34.199 231 142 6 37 257 18 248 9.06e-27 105
MS.gene004321.t1 AT4G17340 33.478 230 143 6 37 256 18 247 8.31e-25 99.8
MS.gene004321.t1 AT3G06100 39.716 141 83 2 114 253 41 180 8.84e-25 98.2
MS.gene004321.t1 AT1G17810 32.794 247 143 7 24 253 13 253 2.29e-23 96.3
MS.gene004321.t1 AT1G73190 30.556 252 140 7 24 253 13 251 7.80e-22 92.4
MS.gene004321.t1 AT2G25810 30.837 227 137 7 19 237 7 221 5.78e-19 84.0
MS.gene004321.t1 AT3G26520 32.381 210 129 5 37 236 21 227 2.97e-18 82.0
MS.gene004321.t1 AT4G35100 30.131 229 137 6 38 247 38 262 1.36e-17 80.9
MS.gene004321.t1 AT4G35100 30.131 229 137 6 38 247 38 262 1.36e-17 80.9
MS.gene004321.t1 AT5G60660 28.270 237 143 6 35 247 36 269 2.10e-17 80.5
MS.gene004321.t1 AT2G36830 32.301 226 136 7 26 240 12 231 2.20e-17 79.7
MS.gene004321.t1 AT2G16850 28.017 232 138 6 38 247 36 260 3.59e-16 77.0
MS.gene004321.t1 AT2G39010 31.217 189 116 5 72 247 81 268 9.08e-16 75.9
MS.gene004321.t1 AT4G01470 30.288 208 138 3 37 237 20 227 1.08e-15 75.1
MS.gene004321.t1 AT3G53420 29.319 191 117 4 72 247 82 269 1.56e-15 75.1
MS.gene004321.t1 AT3G53420 29.319 191 117 4 72 247 82 269 1.56e-15 75.1
MS.gene004321.t1 AT1G01620 31.492 181 106 4 72 237 17 194 1.13e-14 71.6
MS.gene004321.t1 AT3G54820 26.923 234 144 5 38 247 38 268 1.15e-14 72.8
MS.gene004321.t1 AT1G01620 31.492 181 106 4 72 237 89 266 1.97e-14 72.0
MS.gene004321.t1 AT2G37170 28.272 191 119 4 72 247 80 267 3.36e-14 71.2
MS.gene004321.t1 AT3G61430 27.830 212 138 5 41 237 55 266 4.08e-14 71.2
MS.gene004321.t1 AT3G61430 27.830 212 138 5 41 237 55 266 4.08e-14 71.2
MS.gene004321.t1 AT4G00430 31.492 181 106 4 72 237 90 267 5.96e-14 70.9
MS.gene004321.t1 AT2G37170 28.272 191 119 4 72 247 123 310 6.22e-14 71.2
MS.gene004321.t1 AT2G37180 28.272 191 119 4 72 247 80 267 7.04e-14 70.5
MS.gene004321.t1 AT3G47440 29.630 216 142 5 37 244 22 235 9.51e-14 69.7
MS.gene004321.t1 AT2G45960 27.830 212 138 5 41 237 55 266 1.20e-13 69.7
MS.gene004321.t1 AT4G23400 30.939 181 107 4 72 237 90 267 1.80e-13 69.3
MS.gene004321.t1 AT2G45960 28.351 194 127 4 41 222 55 248 7.45e-13 67.8
MS.gene004321.t1 AT2G45960 28.351 194 127 4 41 222 55 248 7.88e-13 67.4

Find 74 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTCTGTTGGTCACATTTC+TGG 0.071303 2.3:-68652815 MS.gene004321:CDS
TATCTTATGTTCGTCATTTC+TGG 0.082482 2.3:-68651983 MS.gene004321:CDS
TCCTGGGATTTCAATTGTTT+GGG 0.161462 2.3:-68652861 MS.gene004321:CDS
AAGAGTGCCTCTTTCCTTAA+AGG 0.183929 2.3:-68650595 MS.gene004321:CDS
TGCTTCCACCAAAAGGTTTC+CGG 0.227485 2.3:-68652759 MS.gene004321:CDS
GGGCCTCACTGTTATGGTTT+TGG 0.229194 2.3:-68652840 MS.gene004321:CDS
TTCATTGATGCTCCAGTAAT+TGG 0.244218 2.3:+68650766 None:intergenic
TAGAGGAATATGGATATATT+TGG 0.245829 2.3:-68650701 MS.gene004321:CDS
ATCCTGGGATTTCAATTGTT+TGG 0.255375 2.3:-68652862 MS.gene004321:CDS
TGCATGACTCATCACAGTTT+TGG 0.271846 2.3:+68653369 None:intergenic
TTCCTTAAAGGAGTAGAAAA+TGG 0.281136 2.3:-68650583 MS.gene004321:CDS
ACGTATTTCTTGATATTTGC+AGG 0.289169 2.3:-68652932 MS.gene004321:CDS
TTTGTTAACAGGCCAATTAC+TGG 0.289918 2.3:-68650778 MS.gene004321:intron
CTGCTTAACCGGAAACCTTT+TGG 0.301395 2.3:+68652751 None:intergenic
TATATAATATCTCAAGTTCT+TGG 0.312865 2.3:-68652127 MS.gene004321:CDS
TGTTTGGGGCCTCACTGTTA+TGG 0.316624 2.3:-68652846 MS.gene004321:CDS
CTTCTAAATGTTATGTTTGC+CGG 0.328211 2.3:-68651149 MS.gene004321:CDS
GCAGGTCCTAAGCTCCTTGC+AGG 0.331255 2.3:+68650742 None:intergenic
ATAAAGAGTTTAGAGGAATA+TGG 0.344662 2.3:-68650711 MS.gene004321:CDS
TTACATTCAAAACCATTTCA+TGG 0.353026 2.3:+68653405 None:intergenic
ACTCGGATCACCTGCTTAAC+CGG 0.396444 2.3:+68652740 None:intergenic
TCCTGCTGGTTCTGATCTCC+AGG 0.406259 2.3:-68652035 MS.gene004321:CDS
ATTGTTCATAAAGAGTTTAG+AGG 0.413252 2.3:-68650718 MS.gene004321:CDS
TTCTGATCTCCAGGCATTTG+TGG 0.415818 2.3:-68652026 MS.gene004321:CDS
TTCTACTCCTTTAAGGAAAG+AGG 0.436744 2.3:+68650588 None:intergenic
ATGAACCCTGCAAGGAGCTT+AGG 0.445185 2.3:-68650748 MS.gene004321:CDS
AGTAGATCCAACTGCAAGTC+CGG 0.451623 2.3:+68651175 None:intergenic
ACACTTAGGCTTATATTCAA+TGG 0.454254 2.3:-68652085 MS.gene004321:CDS
GACAAAGTAGTAACACATCC+TGG 0.460900 2.3:-68652878 MS.gene004321:CDS
CAAAAGGTTTCCGGTTAAGC+AGG 0.462415 2.3:-68652750 MS.gene004321:intron
CTCTTACTAATTTCACGCAC+TGG 0.467398 2.3:+68650613 None:intergenic
AAGCAATGGAAACCATGAAA+TGG 0.472703 2.3:-68653417 MS.gene004321:CDS
GCAAAAGAGGCACATAGTCT+TGG 0.488743 2.3:+68653345 None:intergenic
ACAACTCTTGGTGCCATAGC+TGG 0.490463 2.3:-68650673 MS.gene004321:CDS
TTGGTGCCATAGCTGGTACA+TGG 0.491349 2.3:-68650666 MS.gene004321:CDS
TTGCTCATGCTTCCACCAAA+AGG 0.493038 2.3:-68652766 MS.gene004321:CDS
ATACACACCTTCTGCAAAAG+AGG 0.497346 2.3:+68653332 None:intergenic
TTACTACTTTGTCATTGTCA+AGG 0.498566 2.3:+68652888 None:intergenic
TTCTAAATGTTATGTTTGCC+GGG 0.499731 2.3:-68651148 MS.gene004321:intron
TATTTGGTATCAACAACTCT+TGG 0.500417 2.3:-68650685 MS.gene004321:CDS
AACTCTTTATGAACAATAGC+AGG 0.503476 2.3:+68650724 None:intergenic
TGCAGGGTGTGCTGCTGTTG+TGG 0.511373 2.3:-68652915 MS.gene004321:CDS
CCTGGGATTTCAATTGTTTG+GGG 0.520005 2.3:-68652860 MS.gene004321:CDS
TGAATTCAACCACAAATGCC+TGG 0.521494 2.3:+68652017 None:intergenic
GGAGATTACCGCTCTATTGT+CGG 0.528942 2.3:+68651952 None:intergenic
CCCCAAACAATTGAAATCCC+AGG 0.531908 2.3:+68652860 None:intergenic
TGGTGCCATAGCTGGTACAT+GGG 0.538881 2.3:-68650665 MS.gene004321:CDS
TCGCAGATTGGTGAGTTGGC+CGG 0.540241 2.3:-68651194 MS.gene004321:intron
CGTATTTCTTGATATTTGCA+GGG 0.547320 2.3:-68652931 MS.gene004321:CDS
GCATGAGCAATGGTGACAGC+AGG 0.549452 2.3:+68652779 None:intergenic
TATGTTCGTCATTTCTGGAG+TGG 0.553193 2.3:-68651978 MS.gene004321:CDS
AATATCGCAGATTGGTGAGT+TGG 0.558536 2.3:-68651198 MS.gene004321:intron
GCACTGGCTTGTTTGTGTAC+CGG 0.558893 2.3:+68650629 None:intergenic
TAGGCTTATATTCAATGGCA+AGG 0.559061 2.3:-68652080 MS.gene004321:CDS
CATGGGCATATACTTTCATC+CGG 0.565944 2.3:-68650648 MS.gene004321:CDS
CTTGGATCAACTCTTGCAAG+TGG 0.567738 2.3:-68652109 MS.gene004321:CDS
CTTAAAGGAGTAGAAAATGG+TGG 0.573302 2.3:-68650580 MS.gene004321:CDS
GTAGTTGGTAGCAGAAGTGG+TGG 0.576572 2.3:-68652957 MS.gene004321:intron
GCCTGGAGATCAGAACCAGC+AGG 0.579301 2.3:+68652034 None:intergenic
ATATGCCCATGTACCAGCTA+TGG 0.580208 2.3:+68650660 None:intergenic
ACTTGAGATATTATATAAGC+CGG 0.584944 2.3:+68652133 None:intergenic
GAGTTGGCCGGACTTGCAGT+TGG 0.596194 2.3:-68651182 MS.gene004321:CDS
AATGTAGTTGGTAGCAGAAG+TGG 0.598804 2.3:-68652960 MS.gene004321:intron
ACAAAGTAGTAACACATCCT+GGG 0.600342 2.3:-68652877 MS.gene004321:CDS
GCTGATCATTCATCAAGCAA+TGG 0.606516 2.3:-68653431 MS.gene004321:CDS
GAGCATCAATGAACCCTGCA+AGG 0.617643 2.3:-68650756 MS.gene004321:CDS
AGTTGGTAGCAGAAGTGGTG+GGG 0.626709 2.3:-68652955 MS.gene004321:CDS
CAGGTCCTAAGCTCCTTGCA+GGG 0.629880 2.3:+68650743 None:intergenic
CTCTTGCAAGTGGAACACTT+AGG 0.640873 2.3:-68652099 MS.gene004321:CDS
TAGTTGGTAGCAGAAGTGGT+GGG 0.655474 2.3:-68652956 MS.gene004321:CDS
GATTACCGCTCTATTGTCGG+TGG 0.674328 2.3:+68651955 None:intergenic
TTTGGTGGAAGCATGAGCAA+TGG 0.698455 2.3:+68652769 None:intergenic
AGTGGCCACCGACAATAGAG+CGG 0.719424 2.3:-68651960 MS.gene004321:intron
AAACCAAAACCATAACAGTG+AGG 0.762163 2.3:+68652837 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTGACAAAAAAAATAAA+AGG + chr2.3:68652791-68652810 None:intergenic 10.0%
!! GAGTATTTGTTTATATATAA+GGG + chr2.3:68652082-68652101 None:intergenic 15.0%
!! TATACATTAGTAAAAAAGTA+TGG - chr2.3:68652523-68652542 MS.gene004321:intron 15.0%
!!! AGAGTATTTGTTTATATATA+AGG + chr2.3:68652083-68652102 None:intergenic 15.0%
!!! ATCTTTGTTATTAAAGTATT+AGG + chr2.3:68650854-68650873 None:intergenic 15.0%
!!! GAATTTAATCTCTAAAATAT+CGG - chr2.3:68650752-68650771 MS.gene004321:CDS 15.0%
!!! GATATATGTGATTTTAAATA+TGG - chr2.3:68650981-68651000 MS.gene004321:intron 15.0%
!!! TACTTTAGAGATTAAATTAA+CGG - chr2.3:68650950-68650969 MS.gene004321:intron 15.0%
!! AAGAAAATATGATTGCTTTA+AGG + chr2.3:68651550-68651569 None:intergenic 20.0%
!! AATGTATCTGATTCAAAATT+TGG - chr2.3:68651347-68651366 MS.gene004321:intron 20.0%
!! ACATGTTAATGAATATAATG+TGG - chr2.3:68652659-68652678 MS.gene004321:intron 20.0%
!! CCATATTATGAATTCAAAAA+CGG + chr2.3:68651714-68651733 None:intergenic 20.0%
!! GAATCAATGTTGAAATAAAA+TGG - chr2.3:68651003-68651022 MS.gene004321:intron 20.0%
!! GTACAATTAAGTATACTATT+TGG + chr2.3:68651797-68651816 None:intergenic 20.0%
!! TATATAATATCTCAAGTTCT+TGG - chr2.3:68651877-68651896 MS.gene004321:intron 20.0%
!! TTTGGATAATATGCAAAAAA+TGG + chr2.3:68651779-68651798 None:intergenic 20.0%
!!! CAATAATTTTTTAGATGATG+TGG - chr2.3:68651468-68651487 MS.gene004321:intron 20.0%
!!! GAATATAGTTTTAAAGAACT+CGG + chr2.3:68651284-68651303 None:intergenic 20.0%
!!! TTCTTAATATGTTTAGTTTG+AGG + chr2.3:68652905-68652924 None:intergenic 20.0%
! AAAAAAAGTTCTATGTAGAG+TGG + chr2.3:68650706-68650725 None:intergenic 25.0%
! AAATGACATAATATAGAGCA+TGG + chr2.3:68651674-68651693 None:intergenic 25.0%
! ACTTGAGATATTATATAAGC+CGG + chr2.3:68651874-68651893 None:intergenic 25.0%
! ATAAAGAGTTTAGAGGAATA+TGG - chr2.3:68653293-68653312 MS.gene004321:intron 25.0%
! ATTGTTCATAAAGAGTTTAG+AGG - chr2.3:68653286-68653305 MS.gene004321:intron 25.0%
! GCAAAAAATGGAAAAAGTTT+AGG + chr2.3:68651767-68651786 None:intergenic 25.0%
! TAATCTTATTGAAGAACTCT+CGG + chr2.3:68653138-68653157 None:intergenic 25.0%
! TAGAGGAATATGGATATATT+TGG - chr2.3:68653303-68653322 MS.gene004321:intron 25.0%
! TCTAACATTTACTCTCTTAT+TGG + chr2.3:68653053-68653072 None:intergenic 25.0%
! TGTATTATGTTGAATGTAGT+TGG - chr2.3:68651032-68651051 MS.gene004321:intron 25.0%
! TTACATTCAAAACCATTTCA+TGG + chr2.3:68650602-68650621 None:intergenic 25.0%
! TTGTTTATATATAAGGGCAA+AGG + chr2.3:68652076-68652095 None:intergenic 25.0%
!! AATCCTATATGTTTGTTAAC+AGG - chr2.3:68653215-68653234 MS.gene004321:intron 25.0%
!! GAATATACACAACTTTTCAA+CGG + chr2.3:68652140-68652159 None:intergenic 25.0%
!! TTCATTAACATGTGTTTCTA+AGG + chr2.3:68652652-68652671 None:intergenic 25.0%
!! TTGAGGTTTGATTTATAGTT+AGG + chr2.3:68652888-68652907 None:intergenic 25.0%
!!! ATTGGACATGTATTTTGAAA+CGG - chr2.3:68652582-68652601 MS.gene004321:intron 25.0%
!!! CCGTTTTTGAATTCATAATA+TGG - chr2.3:68651711-68651730 MS.gene004321:intron 25.0%
!!! GAAATGGTTTTGAATGTAAA+TGG - chr2.3:68650603-68650622 MS.gene004321:CDS 25.0%
AAAACGGTTGCATACATATA+CGG + chr2.3:68651698-68651717 None:intergenic 30.0%
AACTCTTTATGAACAATAGC+AGG + chr2.3:68653283-68653302 None:intergenic 30.0%
AATCCATATGCTTCAAAGTT+GGG + chr2.3:68651397-68651416 None:intergenic 30.0%
AATTGCAAATATGTCACAAG+TGG - chr2.3:68652254-68652273 MS.gene004321:intron 30.0%
ACACTTAGGCTTATATTCAA+TGG - chr2.3:68651919-68651938 MS.gene004321:intron 30.0%
ACGTATTTCTTGATATTTGC+AGG - chr2.3:68651072-68651091 MS.gene004321:intron 30.0%
ATAGTGCACATTGCAAAAAT+TGG - chr2.3:68652564-68652583 MS.gene004321:intron 30.0%
CATTCCTCAAATATAGTTAG+TGG - chr2.3:68653101-68653120 MS.gene004321:intron 30.0%
CGTATTTCTTGATATTTGCA+GGG - chr2.3:68651073-68651092 MS.gene004321:intron 30.0%
CTTCTAAATGTTATGTTTGC+CGG - chr2.3:68652855-68652874 MS.gene004321:CDS 30.0%
GCTTTATATGCGAAAAAGTT+GGG - chr2.3:68651312-68651331 MS.gene004321:intron 30.0%
GGAAGAGAAATTTCAATTGA+AGG + chr2.3:68651746-68651765 None:intergenic 30.0%
TAACAGTCAAAATTGTGCAT+TGG + chr2.3:68652383-68652402 None:intergenic 30.0%
TAAGAGAGTAAATGTTAGAG+AGG - chr2.3:68653054-68653073 MS.gene004321:intron 30.0%
TAATCCATATGCTTCAAAGT+TGG + chr2.3:68651398-68651417 None:intergenic 30.0%
TAGAGAGGACATTAGAAAAA+AGG - chr2.3:68653069-68653088 MS.gene004321:intron 30.0%
TATCTTATGTTCGTCATTTC+TGG - chr2.3:68652021-68652040 MS.gene004321:CDS 30.0%
TATTTGGTATCAACAACTCT+TGG - chr2.3:68653319-68653338 MS.gene004321:intron 30.0%
TCTTCAATCTATAATCTTGC+AGG - chr2.3:68651846-68651865 MS.gene004321:intron 30.0%
TGCTTTATATGCGAAAAAGT+TGG - chr2.3:68651311-68651330 MS.gene004321:intron 30.0%
TTACTACTTTGTCATTGTCA+AGG + chr2.3:68651119-68651138 None:intergenic 30.0%
TTATTGGATCAAACTCACAT+GGG + chr2.3:68653037-68653056 None:intergenic 30.0%
TTCCTTAAAGGAGTAGAAAA+TGG - chr2.3:68653421-68653440 MS.gene004321:CDS 30.0%
TTCTAAATGTTATGTTTGCC+GGG - chr2.3:68652856-68652875 MS.gene004321:CDS 30.0%
TTTATAGTTAGGTTCATACC+CGG + chr2.3:68652877-68652896 None:intergenic 30.0%
! ACAATAGTGTTGAAGATTGA+TGG + chr2.3:68652310-68652329 None:intergenic 30.0%
! TTTTTGTCAATATCGCAGAT+TGG - chr2.3:68652798-68652817 MS.gene004321:CDS 30.0%
!! CTTGGACATGTATTTTGAAA+TGG + chr2.3:68652342-68652361 None:intergenic 30.0%
AAACCAAAACCATAACAGTG+AGG + chr2.3:68651170-68651189 None:intergenic 35.0%
AAATATGTCACAAGTGGACA+TGG - chr2.3:68652260-68652279 MS.gene004321:intron 35.0%
AACACTCACTCTCTTATTGA+GGG - chr2.3:68652959-68652978 MS.gene004321:intron 35.0%
AAGCAATGGAAACCATGAAA+TGG - chr2.3:68650587-68650606 MS.gene004321:CDS 35.0%
AATATGTCACAAGTGGACAT+GGG - chr2.3:68652261-68652280 MS.gene004321:intron 35.0%
ACAAAGTAGTAACACATCCT+GGG - chr2.3:68651127-68651146 MS.gene004321:intron 35.0%
AGTTCTATGTAGAGTGGTTT+AGG + chr2.3:68650700-68650719 None:intergenic 35.0%
ATCCTGGGATTTCAATTGTT+TGG - chr2.3:68651142-68651161 MS.gene004321:intron 35.0%
ATCTATAATCTTGCAGGTAC+CGG - chr2.3:68651852-68651871 MS.gene004321:intron 35.0%
CATATGTGAAATGGTTGACT+TGG + chr2.3:68652360-68652379 None:intergenic 35.0%
CTTAAAGGAGTAGAAAATGG+TGG - chr2.3:68653424-68653443 MS.gene004321:CDS 35.0%
CTTATTGGATCAAACTCACA+TGG + chr2.3:68653038-68653057 None:intergenic 35.0%
GTGTCCACTAACTATATTTG+AGG + chr2.3:68653108-68653127 None:intergenic 35.0%
TAACACTCACTCTCTTATTG+AGG - chr2.3:68652958-68652977 MS.gene004321:intron 35.0%
TAGGCTTATATTCAATGGCA+AGG - chr2.3:68651924-68651943 MS.gene004321:intron 35.0%
TATGTCGTCACAATTCAGTT+TGG - chr2.3:68653162-68653181 MS.gene004321:intron 35.0%
TCCTGGGATTTCAATTGTTT+GGG - chr2.3:68651143-68651162 MS.gene004321:intron 35.0%
TGGCCTGTTAACAAACATAT+AGG + chr2.3:68653221-68653240 None:intergenic 35.0%
TTCATTGATGCTCCAGTAAT+TGG + chr2.3:68653241-68653260 None:intergenic 35.0%
TTTGTTAACAGGCCAATTAC+TGG - chr2.3:68653226-68653245 MS.gene004321:intron 35.0%
! CACCATTTTCTACTCCTTTA+AGG + chr2.3:68653426-68653445 None:intergenic 35.0%
! CATATTAACACGTGTCGTTA+AGG + chr2.3:68652767-68652786 None:intergenic 35.0%
! TTCTACTCCTTTAAGGAAAG+AGG + chr2.3:68653419-68653438 None:intergenic 35.0%
!!! ATGGTTTTGGTTTACTCTGT+TGG - chr2.3:68651177-68651196 MS.gene004321:CDS 35.0%
!!! GACATGTATTTTGAAACGGA+GGG - chr2.3:68652586-68652605 MS.gene004321:intron 35.0%
AATATCGCAGATTGGTGAGT+TGG - chr2.3:68652806-68652825 MS.gene004321:CDS 40.0%
ATACACACCTTCTGCAAAAG+AGG + chr2.3:68650675-68650694 None:intergenic 40.0%
CATGGGCATATACTTTCATC+CGG - chr2.3:68653356-68653375 MS.gene004321:CDS 40.0%
CCTGGGATTTCAATTGTTTG+GGG - chr2.3:68651144-68651163 MS.gene004321:intron 40.0%
CTCTTACTAATTTCACGCAC+TGG + chr2.3:68653394-68653413 None:intergenic 40.0%
GAATTGGGTATCACCTAAAG+TGG + chr2.3:68653002-68653021 None:intergenic 40.0%
GACAAAGTAGTAACACATCC+TGG - chr2.3:68651126-68651145 MS.gene004321:intron 40.0%
GATGTTATGCGTGACTATGA+TGG - chr2.3:68651433-68651452 MS.gene004321:intron 40.0%
GCTGATCATTCATCAAGCAA+TGG - chr2.3:68650573-68650592 MS.gene004321:CDS 40.0%
TACTCTGTTGGTCACATTTC+TGG - chr2.3:68651189-68651208 MS.gene004321:CDS 40.0%
TCTTATTGAGGGAACTCATG+TGG - chr2.3:68652970-68652989 MS.gene004321:intron 40.0%
TGAATTCAACCACAAATGCC+TGG + chr2.3:68651990-68652009 None:intergenic 40.0%
TTCGCATATAAAGCACACGA+CGG + chr2.3:68651306-68651325 None:intergenic 40.0%
! GCATGACTCATCACAGTTTT+GGG + chr2.3:68650637-68650656 None:intergenic 40.0%
! GTGCATTGGCATATGTGAAA+TGG + chr2.3:68652369-68652388 None:intergenic 40.0%
! TATGTTCGTCATTTCTGGAG+TGG - chr2.3:68652026-68652045 MS.gene004321:CDS 40.0%
! TGCATGACTCATCACAGTTT+TGG + chr2.3:68650638-68650657 None:intergenic 40.0%
!! AAGAGTGCCTCTTTCCTTAA+AGG - chr2.3:68653409-68653428 MS.gene004321:CDS 40.0%
!! AATGTAGTTGGTAGCAGAAG+TGG - chr2.3:68651044-68651063 MS.gene004321:intron 40.0%
!! CAGGAAGTGTTCCTGAAAAA+TGG + chr2.3:68651954-68651973 None:intergenic 40.0%
!! GGCAAGGAAAACCATTTTTC+AGG - chr2.3:68651940-68651959 MS.gene004321:intron 40.0%
!! TGACCCAACTTTGAAGCATA+TGG - chr2.3:68651391-68651410 MS.gene004321:intron 40.0%
!!! GGACATGTATTTTGAAACGG+AGG - chr2.3:68652585-68652604 MS.gene004321:intron 40.0%
AGTAGATCCAACTGCAAGTC+CGG + chr2.3:68652832-68652851 None:intergenic 45.0%
ATATGCCCATGTACCAGCTA+TGG + chr2.3:68653347-68653366 None:intergenic 45.0%
CAAAAGGTTTCCGGTTAAGC+AGG - chr2.3:68651254-68651273 MS.gene004321:intron 45.0%
CATGTGGATCACACCACTTT+AGG - chr2.3:68652986-68653005 MS.gene004321:intron 45.0%
CCCCAAACAATTGAAATCCC+AGG + chr2.3:68651147-68651166 None:intergenic 45.0%
CTCTTGCAAGTGGAACACTT+AGG - chr2.3:68651905-68651924 MS.gene004321:intron 45.0%
CTTGGATCAACTCTTGCAAG+TGG - chr2.3:68651895-68651914 MS.gene004321:intron 45.0%
GGAGATTACCGCTCTATTGT+CGG + chr2.3:68652055-68652074 None:intergenic 45.0%
TGCTTCCACCAAAAGGTTTC+CGG - chr2.3:68651245-68651264 MS.gene004321:intron 45.0%
TTCTGATCTCCAGGCATTTG+TGG - chr2.3:68651978-68651997 MS.gene004321:CDS 45.0%
TTGCTCATGCTTCCACCAAA+AGG - chr2.3:68651238-68651257 MS.gene004321:intron 45.0%
! CTATGTGCCTCTTTTGCAGA+AGG - chr2.3:68650665-68650684 MS.gene004321:CDS 45.0%
! CTGCTTAACCGGAAACCTTT+TGG + chr2.3:68651256-68651275 None:intergenic 45.0%
! CTTAACCGGAAACCTTTTGG+TGG + chr2.3:68651253-68651272 None:intergenic 45.0%
! GCAAAAGAGGCACATAGTCT+TGG + chr2.3:68650662-68650681 None:intergenic 45.0%
! TTTGGTGGAAGCATGAGCAA+TGG + chr2.3:68651238-68651257 None:intergenic 45.0%
!! GGGTCTCACACTTTGGAATT+GGG + chr2.3:68653017-68653036 None:intergenic 45.0%
!! TAGTTGGTAGCAGAAGTGGT+GGG - chr2.3:68651048-68651067 MS.gene004321:intron 45.0%
!! TGGGTCTCACACTTTGGAAT+TGG + chr2.3:68653018-68653037 None:intergenic 45.0%
!! TTTTCAGGAACACTTCCTGC+TGG - chr2.3:68651955-68651974 MS.gene004321:intron 45.0%
!!! ATATTTTTTTTTTTGAAAAT+TGG - chr2.3:68652684-68652703 MS.gene004321:intron 5.0%
!!! TATTTTTTTTTTTGAAAATT+GGG - chr2.3:68652685-68652704 MS.gene004321:intron 5.0%
ACTCGGATCACCTGCTTAAC+CGG + chr2.3:68651267-68651286 None:intergenic 50.0%
ATGAACCCTGCAAGGAGCTT+AGG - chr2.3:68653256-68653275 MS.gene004321:intron 50.0%
CTCACATGGGTCTCACACTT+TGG + chr2.3:68653024-68653043 None:intergenic 50.0%
GAGCATCAATGAACCCTGCA+AGG - chr2.3:68653248-68653267 MS.gene004321:intron 50.0%
GATTACCGCTCTATTGTCGG+TGG + chr2.3:68652052-68652071 None:intergenic 50.0%
! GGGCCTCACTGTTATGGTTT+TGG - chr2.3:68651164-68651183 MS.gene004321:CDS 50.0%
! TGTTTGGGGCCTCACTGTTA+TGG - chr2.3:68651158-68651177 MS.gene004321:CDS 50.0%
!! ACAACTCTTGGTGCCATAGC+TGG - chr2.3:68653331-68653350 MS.gene004321:intron 50.0%
!! AGTTGGTAGCAGAAGTGGTG+GGG - chr2.3:68651049-68651068 MS.gene004321:intron 50.0%
!! GCACTGGCTTGTTTGTGTAC+CGG + chr2.3:68653378-68653397 None:intergenic 50.0%
!! GTAGTTGGTAGCAGAAGTGG+TGG - chr2.3:68651047-68651066 MS.gene004321:intron 50.0%
!! TGGTGCCATAGCTGGTACAT+GGG - chr2.3:68653339-68653358 MS.gene004321:intron 50.0%
!! TTGGTGCCATAGCTGGTACA+TGG - chr2.3:68653338-68653357 MS.gene004321:intron 50.0%
AGTGGCCACCGACAATAGAG+CGG - chr2.3:68652044-68652063 MS.gene004321:CDS 55.0%
CAGGTCCTAAGCTCCTTGCA+GGG + chr2.3:68653264-68653283 None:intergenic 55.0%
GCATGAGCAATGGTGACAGC+AGG + chr2.3:68651228-68651247 None:intergenic 55.0%
! TCGCAGATTGGTGAGTTGGC+CGG - chr2.3:68652810-68652829 MS.gene004321:CDS 55.0%
!! TCCTGCTGGTTCTGATCTCC+AGG - chr2.3:68651969-68651988 MS.gene004321:CDS 55.0%
GAGTTGGCCGGACTTGCAGT+TGG - chr2.3:68652822-68652841 MS.gene004321:CDS 60.0%
GCAGGTCCTAAGCTCCTTGC+AGG + chr2.3:68653265-68653284 None:intergenic 60.0%
GCCTGGAGATCAGAACCAGC+AGG + chr2.3:68651973-68651992 None:intergenic 60.0%
! TGCAGGGTGTGCTGCTGTTG+TGG - chr2.3:68651089-68651108 MS.gene004321:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 68650570 68653456 68650570 ID=MS.gene004321
chr2.3 mRNA 68650570 68653456 68650570 ID=MS.gene004321.t1;Parent=MS.gene004321
chr2.3 exon 68653340 68653456 68653340 ID=MS.gene004321.t1.exon1;Parent=MS.gene004321.t1
chr2.3 CDS 68653340 68653456 68653340 ID=cds.MS.gene004321.t1;Parent=MS.gene004321.t1
chr2.3 exon 68652751 68652975 68652751 ID=MS.gene004321.t1.exon2;Parent=MS.gene004321.t1
chr2.3 CDS 68652751 68652975 68652751 ID=cds.MS.gene004321.t1;Parent=MS.gene004321.t1
chr2.3 exon 68651961 68652158 68651961 ID=MS.gene004321.t1.exon3;Parent=MS.gene004321.t1
chr2.3 CDS 68651961 68652158 68651961 ID=cds.MS.gene004321.t1;Parent=MS.gene004321.t1
chr2.3 exon 68651149 68651210 68651149 ID=MS.gene004321.t1.exon4;Parent=MS.gene004321.t1
chr2.3 CDS 68651149 68651210 68651149 ID=cds.MS.gene004321.t1;Parent=MS.gene004321.t1
chr2.3 exon 68650570 68650789 68650570 ID=MS.gene004321.t1.exon5;Parent=MS.gene004321.t1
chr2.3 CDS 68650570 68650789 68650570 ID=cds.MS.gene004321.t1;Parent=MS.gene004321.t1
Gene Sequence

>MS.gene004321

ATGGCTGATCATTCATCAAGCAATGGAAACCATGAAATGGTTTTGAATGTAAATGGTGATGCTTCCCAAAACTGTGATGAGTCATGCATCCAAGACTATGTGCCTCTTTTGCAGAAGGTGTGTATCTCCTAAACCACTCTACATAGAACTTTTTTTTTTTTGTTGATAATAACTATGAATATGAATTTAATCTCTAAAATATCGGTCTTTAATTTTATTTTTGAAGTAAATAAAATTCGTTGTCATAGTCTCTATAGTATTTGAAAGTTATTATATTAATTCCTAATACTTTAATAACAAAGATTAAAGTGACGAATTTCATATACTTTAAAAATGTTTATTACTTTTATATGCTTTAAAGATTTAATTAGAGAGAAAAATACTTTAGAGATTAAATTAACGGTTTATTCTGATATATGTGATTTTAAATATGGAATCAATGTTGAAATAAAATGGTTTGATTGTATTATGTTGAATGTAGTTGGTAGCAGAAGTGGTGGGGACGTATTTCTTGATATTTGCAGGGTGTGCTGCTGTTGTGGTGAACCTTGACAATGACAAAGTAGTAACACATCCTGGGATTTCAATTGTTTGGGGCCTCACTGTTATGGTTTTGGTTTACTCTGTTGGTCACATTTCTGGTGCTCATTTCAATCCTGCTGTCACCATTGCTCATGCTTCCACCAAAAGGTTTCCGGTTAAGCAGGTGATCCGAGTTCTTTAAAACTATATTCCGTCGTGTGCTTTATATGCGAAAAAGTTGGGTCAACAAAAATTAATGTATCTGATTCAAAATTTGGATCAAATACATAAATTTTTACTGACCCAACTTTGAAGCATATGGATTATTTTATTTTGAAAACGATGTTATGCGTGACTATGATGGTACACATTTATTCAATAATTTTTTAGATGATGTGGTAAATGAAAATATAATTATTACACTTGAATTTTCTTGAAAATTACTTGTGTTAGCTCCTTAAAGCAATCATATTTTCTTTAAAGAAAATGTTAACTGCTCTAAAAGCACTTGATAAGAAGTTTAAAGTAGAATTTTTTTTCTATAAGATTGATTTTACATATAAAAAAGCAAAAGCTAAACCATGCTCTATATTATGTCATTTTCCGTATATGTATGCAACCGTTTTTGAATTCATAATATGGTTGAATTGACCTTCAATTGAAATTTCTCTTCCTAAACTTTTTCCATTTTTTGCATATTATCCAAATAGTATACTTAATTGTACTAGTTTGTCTAAAAAAATTGTTGAGTTTTTCTTCAATCTATAATCTTGCAGGTACCGGCTTATATAATATCTCAAGTTCTTGGATCAACTCTTGCAAGTGGAACACTTAGGCTTATATTCAATGGCAAGGAAAACCATTTTTCAGGAACACTTCCTGCTGGTTCTGATCTCCAGGCATTTGTGGTTGAATTCATAATCACTTTTTATCTTATGTTCGTCATTTCTGGAGTGGCCACCGACAATAGAGCGGTAATCTCCTTTGCCCTTATATATAAACAAATACTCTTGTATTGAAAAGTGTTGAAATATTGTATATTCAACCGTTGAAAAGTTGTGTATATTCAACTTTTTGAAAATAAAAAATTTGTTATTTTCAATGAAAAGTTTTTACTTTCTTATATTCTTAACTAATTAGCATGACTCTTAAAATATGTATAAATTGCAAATATGTCACAAGTGGACATGGGCAGCAAATTCTACATTTCAATGTCCATCAATCTTCAACACTATTGTATACTCGCTCCATTTCAAAATACATGTCCAAGTCAACCATTTCACATATGCCAATGCACAATTTTGACTGTTAATATATTTAATTATCTAAAGTAAAAAATTATAAAAATTTAATAAAATTATAAAAATTTAATATTTTGAAAATATTTGTCGAGACAAATCAAACAACATCTTACATGCTAATATTTGTATTTATACATTAGTAAAAAAGTATGGTCAAAGTAAGTCAAGTGAATAGTGCACATTGCAAAAATTGGACATGTATTTTGAAACGGAGGGAGTTATAGATTTTGTTATTTTAGTGAAATGCAAACATGTGCCTTAGAAACACATGTTAATGAATATAATGTGGATATATTTTTTTTTTTGAAAATTGGGCATTTAATTTTTTAAATATTTAAAAGTTGTTTTCTGCAATGTAAATGTTCTACATGCCTTAACGACACGTGTTAATATGACCTTTTATTTTTTTTGTCAATATCGCAGATTGGTGAGTTGGCCGGACTTGCAGTTGGATCTACTGTGCTTCTAAATGTTATGTTTGCCGGGTATGAACCTAACTATAAATCAAACCTCAAACTAAACATATTAAGAAATGCTAGAAACATTCGCTCTCTAACACTATTTTTAACACTCACTCTCTTATTGAGGGAACTCATGTGGATCACACCACTTTAGGTGATACCCAATTCCAAAGTGTGAGACCCATGTGAGTTTGATCCAATAAGAGAGTAAATGTTAGAGAGGACATTAGAAAAAAGGTGCTCAGATCATTCCTCAAATATAGTTAGTGGACACTTTTACACCGAGAGTTCTTCAATAAGATTACAATTATGTCGTCACAATTCAGTTTGGTCAAATTACTTAGAACTTAATCATGCATGCAATCCTATATGTTTGTTAACAGGCCAATTACTGGAGCATCAATGAACCCTGCAAGGAGCTTAGGACCTGCTATTGTTCATAAAGAGTTTAGAGGAATATGGATATATTTGGTATCAACAACTCTTGGTGCCATAGCTGGTACATGGGCATATACTTTCATCCGGTACACAAACAAGCCAGTGCGTGAAATTAGTAAGAGTGCCTCTTTCCTTAAAGGAGTAGAAAATGGTGGAGCTAAGTGA
Protein sequence

>MS.gene004321.t1

MADHSSSNGNHEMVLNVNGDASQNCDESCIQDYVPLLQKLVAEVVGTYFLIFAGCAAVVVNLDNDKVVTHPGISIVWGLTVMVLVYSVGHISGAHFNPAVTIAHASTKRFPVKQVPAYIISQVLGSTLASGTLRLIFNGKENHFSGTLPAGSDLQAFVVEFIITFYLMFVISGVATDNRAIGELAGLAVGSTVLLNVMFAGPITGASMNPARSLGPAIVHKEFRGIWIYLVSTTLGAIAGTWAYTFIRYTNKPVREISKSASFLKGVENGGAK