AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene03094

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene03094.t1 MTR_2g017570 97.436 273 7 0 1 273 1 273 0.0 545
MS.gene03094.t1 MTR_2g017610 97.436 273 7 0 1 273 1 273 0.0 543
MS.gene03094.t1 MTR_2g017590 81.439 264 49 0 7 270 5 268 9.08e-164 455
MS.gene03094.t1 MTR_2g017620 78.571 266 57 0 1 266 1 266 3.95e-155 433
MS.gene03094.t1 MTR_8g087720 76.866 268 62 0 1 268 1 268 1.42e-153 429
MS.gene03094.t1 MTR_2g017620 79.762 252 51 0 15 266 1 252 3.16e-149 417
MS.gene03094.t1 MTR_8g087710 70.480 271 80 0 3 273 4 274 2.95e-142 400
MS.gene03094.t1 MTR_2g017660 69.485 272 76 2 1 265 1 272 1.79e-140 396
MS.gene03094.t1 MTR_8g087720 78.109 201 44 0 1 201 1 201 1.02e-111 320
MS.gene03094.t1 MTR_2g017620 82.703 185 32 0 82 266 1 185 7.27e-111 318
MS.gene03094.t1 MTR_4g084880 58.025 243 100 1 32 272 29 271 1.50e-102 300
MS.gene03094.t1 MTR_8g087720 75.000 184 46 0 1 184 1 184 4.69e-97 283
MS.gene03094.t1 MTR_5g079570 52.720 239 104 3 18 253 22 254 2.80e-84 253
MS.gene03094.t1 MTR_3g008920 53.556 239 108 2 18 256 8 243 9.51e-84 252
MS.gene03094.t1 MTR_4g006730 49.402 251 118 3 21 265 58 305 2.05e-70 219
MS.gene03094.t1 MTR_7g010650 47.085 223 115 2 31 251 28 249 8.87e-69 214
MS.gene03094.t1 MTR_2g104940 51.163 215 100 2 36 248 78 289 1.53e-67 212
MS.gene03094.t1 MTR_5g063930 46.947 262 116 7 15 260 5 259 3.33e-67 209
MS.gene03094.t1 MTR_1g097840 46.800 250 114 4 26 259 54 300 3.30e-66 208
MS.gene03094.t1 MTR_4g006630 50.226 221 101 3 51 265 1 218 4.25e-64 200
MS.gene03094.t1 MTR_4g006730 50.226 221 101 3 51 265 1 218 5.34e-64 200
MS.gene03094.t1 MTR_4g006730 50.820 183 85 2 21 201 58 237 5.21e-47 158
MS.gene03094.t1 MTR_1g070385 39.906 213 127 1 40 252 64 275 1.76e-44 160
MS.gene03094.t1 MTR_1g097840 47.849 186 84 3 26 201 54 236 1.25e-43 149
MS.gene03094.t1 MTR_7g010650 43.137 153 84 2 31 181 28 179 1.70e-37 131
MS.gene03094.t1 MTR_4g006650 47.222 144 62 3 39 180 5 136 6.71e-27 107
MS.gene03094.t1 MTR_8g013680 32.850 207 132 3 37 236 18 224 1.43e-25 101
MS.gene03094.t1 MTR_1g077540 31.950 241 146 6 24 253 10 243 2.03e-24 99.0
MS.gene03094.t1 MTR_2g101370 30.242 248 161 6 18 256 3 247 8.37e-23 94.4
MS.gene03094.t1 MTR_5g012810 33.913 230 141 7 37 256 18 246 1.41e-22 93.6
MS.gene03094.t1 MTR_6g069040 29.583 240 153 6 24 253 10 243 3.16e-22 92.8
MS.gene03094.t1 MTR_1g006490 32.143 224 136 7 19 236 7 220 4.15e-22 92.4
MS.gene03094.t1 MTR_1g006590 32.000 250 150 9 19 258 7 246 2.33e-21 90.5
MS.gene03094.t1 MTR_4g063090 32.159 227 140 7 43 256 26 251 2.34e-17 79.7
MS.gene03094.t1 MTR_7g103030 30.667 225 146 6 37 252 20 243 3.57e-17 79.0
MS.gene03094.t1 MTR_3g118010 28.384 229 141 5 38 247 37 261 1.88e-16 77.8
MS.gene03094.t1 MTR_2g094270 30.899 178 111 3 72 237 84 261 2.11e-16 77.4
MS.gene03094.t1 MTR_5g082070 31.019 216 127 7 41 237 58 270 4.06e-16 76.6
MS.gene03094.t1 MTR_5g010150 30.047 213 133 5 41 237 55 267 1.24e-15 75.5
MS.gene03094.t1 MTR_7g101190 29.412 221 135 6 38 237 39 259 1.28e-15 75.5
MS.gene03094.t1 MTR_8g098375 30.516 213 132 6 41 237 55 267 1.54e-15 75.1
MS.gene03094.t1 MTR_1g115405 32.227 211 130 5 37 237 20 227 1.58e-15 74.7
MS.gene03094.t1 MTR_3g070210 28.638 213 136 5 41 237 58 270 1.11e-14 72.8
MS.gene03094.t1 MTR_4g415300 29.245 212 135 5 41 237 59 270 2.15e-14 72.0
MS.gene03094.t1 MTR_2g094270 30.573 157 100 2 72 219 84 240 7.55e-14 70.1
MS.gene03094.t1 MTR_3g070210 28.205 195 127 4 41 222 58 252 2.72e-13 68.6
MS.gene03094.t1 MTR_1g095070 28.571 182 112 4 81 247 25 203 3.05e-13 67.8
MS.gene03094.t1 MTR_7g023350 28.740 254 147 9 11 251 4 236 7.53e-13 67.0
MS.gene03094.t1 MTR_2g060360 26.639 244 164 5 37 273 18 253 9.83e-13 66.6
MS.gene03094.t1 MTR_4g059390 29.282 181 110 5 72 237 82 259 7.15e-12 64.7
MS.gene03094.t1 MTR_1g095070 28.571 154 95 3 81 222 25 175 4.28e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene03094.t1 AT4G18910 70.817 257 68 1 24 273 38 294 1.39e-131 374
MS.gene03094.t1 AT4G19030 68.504 254 73 1 24 270 41 294 1.48e-125 359
MS.gene03094.t1 AT5G37820 58.431 255 103 1 18 269 20 274 1.12e-99 293
MS.gene03094.t1 AT5G37820 58.431 255 103 1 18 269 56 310 4.46e-99 293
MS.gene03094.t1 AT5G37810 54.015 274 119 3 1 268 1 273 3.52e-98 289
MS.gene03094.t1 AT4G18910 75.135 185 39 1 24 201 38 222 2.17e-92 272
MS.gene03094.t1 AT2G34390 51.748 286 120 3 1 269 1 285 1.93e-90 270
MS.gene03094.t1 AT1G31885 53.036 247 109 1 20 259 25 271 3.33e-90 270
MS.gene03094.t1 AT4G19030 70.109 184 48 1 24 200 41 224 6.09e-88 261
MS.gene03094.t1 AT2G34390 55.462 238 98 2 39 269 7 243 4.47e-86 257
MS.gene03094.t1 AT1G31885 54.839 217 91 1 50 259 1 217 1.61e-83 251
MS.gene03094.t1 AT4G19030 67.879 165 46 1 24 181 41 205 8.26e-76 230
MS.gene03094.t1 AT2G34390 54.128 218 82 3 1 201 1 217 8.86e-69 212
MS.gene03094.t1 AT4G10380 47.755 245 113 4 27 259 63 304 1.22e-67 212
MS.gene03094.t1 AT1G80760 52.036 221 101 2 34 252 76 293 1.29e-67 212
MS.gene03094.t1 AT2G34390 52.500 200 77 3 1 183 1 199 9.86e-60 189
MS.gene03094.t1 AT3G06100 40.367 218 128 2 37 253 45 261 2.07e-44 151
MS.gene03094.t1 AT3G06100 40.654 214 125 2 41 253 1 213 4.65e-44 149
MS.gene03094.t1 AT1G31885 56.842 95 41 0 20 114 25 119 2.94e-31 113
MS.gene03094.t1 AT2G29870 48.718 117 60 0 153 269 20 136 1.45e-30 111
MS.gene03094.t1 AT3G16240 34.783 207 128 3 37 236 18 224 3.47e-28 108
MS.gene03094.t1 AT5G47450 34.199 231 142 6 37 257 18 248 8.96e-27 105
MS.gene03094.t1 AT4G17340 33.478 230 143 6 37 256 18 247 8.14e-25 99.8
MS.gene03094.t1 AT3G06100 39.716 141 83 2 114 253 41 180 9.12e-25 98.2
MS.gene03094.t1 AT1G17810 32.794 247 143 7 24 253 13 253 7.85e-24 97.8
MS.gene03094.t1 AT1G73190 30.556 252 140 7 24 253 13 251 2.50e-22 93.6
MS.gene03094.t1 AT2G25810 30.837 227 137 7 19 237 7 221 4.09e-19 84.3
MS.gene03094.t1 AT3G26520 32.381 210 129 5 37 236 21 227 2.66e-18 82.4
MS.gene03094.t1 AT2G36830 32.018 228 138 7 24 240 10 231 1.12e-17 80.5
MS.gene03094.t1 AT4G35100 30.131 229 137 6 38 247 38 262 1.33e-17 80.9
MS.gene03094.t1 AT4G35100 30.131 229 137 6 38 247 38 262 1.33e-17 80.9
MS.gene03094.t1 AT5G60660 28.270 237 143 6 35 247 36 269 2.04e-17 80.5
MS.gene03094.t1 AT2G16850 28.017 232 138 6 38 247 36 260 3.85e-16 76.6
MS.gene03094.t1 AT2G39010 31.217 189 116 5 72 247 81 268 8.90e-16 75.9
MS.gene03094.t1 AT4G01470 35.065 154 97 2 87 237 74 227 1.05e-15 75.1
MS.gene03094.t1 AT3G53420 29.319 191 117 4 72 247 82 269 1.50e-15 75.1
MS.gene03094.t1 AT3G53420 29.319 191 117 4 72 247 82 269 1.50e-15 75.1
MS.gene03094.t1 AT3G54820 26.923 234 144 5 38 247 38 268 1.02e-14 72.8
MS.gene03094.t1 AT1G01620 31.492 181 106 4 72 237 17 194 1.10e-14 71.6
MS.gene03094.t1 AT1G01620 31.492 181 106 4 72 237 89 266 2.04e-14 72.0
MS.gene03094.t1 AT2G37170 28.272 191 119 4 72 247 80 267 3.33e-14 71.2
MS.gene03094.t1 AT3G61430 27.830 212 138 5 41 237 55 266 3.63e-14 71.2
MS.gene03094.t1 AT3G61430 27.830 212 138 5 41 237 55 266 3.63e-14 71.2
MS.gene03094.t1 AT4G00430 31.492 181 106 4 72 237 90 267 5.26e-14 70.9
MS.gene03094.t1 AT2G37170 28.272 191 119 4 72 247 123 310 5.87e-14 71.2
MS.gene03094.t1 AT2G37180 28.272 191 119 4 72 247 80 267 6.97e-14 70.5
MS.gene03094.t1 AT3G47440 29.630 216 142 5 37 244 22 235 7.90e-14 70.1
MS.gene03094.t1 AT2G45960 27.830 212 138 5 41 237 55 266 9.60e-14 70.1
MS.gene03094.t1 AT4G23400 30.939 181 107 4 72 237 90 267 1.68e-13 69.3
MS.gene03094.t1 AT2G45960 28.351 194 127 4 41 222 55 248 6.82e-13 67.4
MS.gene03094.t1 AT2G45960 28.351 194 127 4 41 222 55 248 6.96e-13 67.8

Find 74 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTCTGTTGGTCACATTTC+TGG 0.071303 2.3:-68892253 MS.gene03094:CDS
TATCTTATGTTCGTCATTTC+TGG 0.082482 2.3:-68891418 MS.gene03094:CDS
AAGAGTGCCTCTTTCCTTAA+AGG 0.183929 2.3:-68890305 MS.gene03094:CDS
TCCTGGGATTTCAATTGTTT+GGG 0.185357 2.3:-68892299 MS.gene03094:CDS
TTCATTGATGCTCCAGTAAT+TGG 0.244218 2.3:+68890476 None:intergenic
TAGAGGAATATGGATATATT+TGG 0.245829 2.3:-68890411 MS.gene03094:CDS
TGCATGACTCATCACACTTT+TGG 0.246215 2.3:+68892800 None:intergenic
ATCCTGGGATTTCAATTGTT+TGG 0.255375 2.3:-68892300 MS.gene03094:CDS
GGGTCTCACTGTTATGGTTT+TGG 0.266153 2.3:-68892278 MS.gene03094:CDS
TGCATCCACCAAAAGGTTTC+CGG 0.271933 2.3:-68892197 MS.gene03094:CDS
TTCCTTAAAGGAGTAGAAAA+TGG 0.281136 2.3:-68890293 MS.gene03094:CDS
ACGTACTTCTTGATATTTGC+AGG 0.286199 2.3:-68892370 MS.gene03094:CDS
TTTGTTAACAGGCCAATTAC+TGG 0.289918 2.3:-68890488 MS.gene03094:intron
CTGCTTAACCGGAAACCTTT+TGG 0.301395 2.3:+68892189 None:intergenic
TGTTTGGGGTCTCACTGTTA+TGG 0.308145 2.3:-68892284 MS.gene03094:CDS
TATATAATATCTCAAGTTCT+TGG 0.312865 2.3:-68891562 MS.gene03094:CDS
CTTCTAAATGTTATGTTTGC+CGG 0.328211 2.3:-68890859 MS.gene03094:CDS
GCAGGTCCTAAGCTCCTTGC+AGG 0.331255 2.3:+68890452 None:intergenic
ATAAAGAGTTTAGAGGAATA+TGG 0.344662 2.3:-68890421 MS.gene03094:CDS
TTACATTCAAAACCATTTCA+TGG 0.353026 2.3:+68892836 None:intergenic
ACTCGGATCACCTGCTTAAC+CGG 0.396444 2.3:+68892178 None:intergenic
TCCTGCTGGTTCTGATCTCC+AGG 0.406259 2.3:-68891470 MS.gene03094:CDS
ATTGTTCATAAAGAGTTTAG+AGG 0.413252 2.3:-68890428 MS.gene03094:CDS
TTCTGATCTCCAGGCATTTG+TGG 0.415818 2.3:-68891461 MS.gene03094:CDS
TTCTACTCCTTTAAGGAAAG+AGG 0.436744 2.3:+68890298 None:intergenic
ATGAACCCTGCAAGGAGCTT+AGG 0.445185 2.3:-68890458 MS.gene03094:CDS
AGTAGATCCAACTGCAAGTC+CGG 0.451623 2.3:+68890885 None:intergenic
ACACTTAGGCTTATATTCAA+TGG 0.454254 2.3:-68891520 MS.gene03094:CDS
GACAAAGTGGTAACACATCC+TGG 0.456746 2.3:-68892316 MS.gene03094:CDS
CAAAAGGTTTCCGGTTAAGC+AGG 0.462415 2.3:-68892188 MS.gene03094:intron
CTCTTACTAATTTCACGCAC+TGG 0.467398 2.3:+68890323 None:intergenic
AAGCAATGGAAACCATGAAA+TGG 0.472703 2.3:-68892848 MS.gene03094:CDS
GCAAAAGAGGCACATAGTCT+TGG 0.488743 2.3:+68892776 None:intergenic
ACAACTCTTGGTGCCATAGC+TGG 0.490463 2.3:-68890383 MS.gene03094:CDS
TTGGTGCCATAGCTGGTACA+TGG 0.491349 2.3:-68890376 MS.gene03094:CDS
ATACACACCTTCTGCAAAAG+AGG 0.497346 2.3:+68892763 None:intergenic
TTCTAAATGTTATGTTTGCC+GGG 0.499731 2.3:-68890858 MS.gene03094:intron
TATTTGGTATCAACAACTCT+TGG 0.500417 2.3:-68890395 MS.gene03094:CDS
AACTCTTTATGAACAATAGC+AGG 0.503476 2.3:+68890434 None:intergenic
TGCAGGGTGTGCTGCTGTTG+TGG 0.511373 2.3:-68892353 MS.gene03094:CDS
TTGCTCATGCATCCACCAAA+AGG 0.514247 2.3:-68892204 MS.gene03094:CDS
CCTGGGATTTCAATTGTTTG+GGG 0.520676 2.3:-68892298 MS.gene03094:CDS
TGAATTCAACCACAAATGCC+TGG 0.521494 2.3:+68891452 None:intergenic
TTACCACTTTGTCATTGTCA+AGG 0.527510 2.3:+68892326 None:intergenic
GGAGATTACCGCTCTATTGT+CGG 0.528942 2.3:+68891387 None:intergenic
GTAGTTGGTAGCAGAGGTTG+TGG 0.535028 2.3:-68892395 MS.gene03094:intron
TGGTGCCATAGCTGGTACAT+GGG 0.538881 2.3:-68890375 MS.gene03094:CDS
TCGCAGATTGGTGAGTTGGC+CGG 0.540241 2.3:-68890904 MS.gene03094:intron
CCCCAAACAATTGAAATCCC+AGG 0.550515 2.3:+68892298 None:intergenic
TATGTTCGTCATTTCTGGAG+TGG 0.553193 2.3:-68891413 MS.gene03094:CDS
GCATGAGCAATGGTGACAGC+AGG 0.555499 2.3:+68892217 None:intergenic
TAGTTGGTAGCAGAGGTTGT+GGG 0.558311 2.3:-68892394 MS.gene03094:CDS
AATATCGCAGATTGGTGAGT+TGG 0.558536 2.3:-68890908 MS.gene03094:intron
GCACTGGCTTGTTTGTGTAC+CGG 0.558893 2.3:+68890339 None:intergenic
TAGGCTTATATTCAATGGCA+AGG 0.559061 2.3:-68891515 MS.gene03094:CDS
CATGGGCATATACTTTCATC+CGG 0.565944 2.3:-68890358 MS.gene03094:CDS
CTTGGATCAACTCTTGCAAG+TGG 0.567738 2.3:-68891544 MS.gene03094:CDS
CTTAAAGGAGTAGAAAATGG+TGG 0.573302 2.3:-68890290 MS.gene03094:CDS
CGTACTTCTTGATATTTGCA+GGG 0.575700 2.3:-68892369 MS.gene03094:CDS
GCCTGGAGATCAGAACCAGC+AGG 0.579301 2.3:+68891469 None:intergenic
ATATGCCCATGTACCAGCTA+TGG 0.580208 2.3:+68890370 None:intergenic
ACTTGAGATATTATATAAGC+CGG 0.584944 2.3:+68891568 None:intergenic
GAGTTGGCCGGACTTGCAGT+TGG 0.596194 2.3:-68890892 MS.gene03094:CDS
GCTGATCATTCATCAAGCAA+TGG 0.606516 2.3:-68892862 MS.gene03094:CDS
ACAAAGTGGTAACACATCCT+GGG 0.613941 2.3:-68892315 MS.gene03094:CDS
GAGCATCAATGAACCCTGCA+AGG 0.617643 2.3:-68890466 MS.gene03094:CDS
CAGGTCCTAAGCTCCTTGCA+GGG 0.629880 2.3:+68890453 None:intergenic
CTCTTGCAAGTGGAACACTT+AGG 0.640873 2.3:-68891534 MS.gene03094:CDS
TTGAATGTAGTTGGTAGCAG+AGG 0.649135 2.3:-68892401 MS.gene03094:intron
AGTTGGTAGCAGAGGTTGTG+GGG 0.653045 2.3:-68892393 MS.gene03094:CDS
TTTGGTGGATGCATGAGCAA+TGG 0.663155 2.3:+68892207 None:intergenic
GATTACCGCTCTATTGTCGG+TGG 0.674328 2.3:+68891390 None:intergenic
GAACCTTGACAATGACAAAG+TGG 0.704582 2.3:-68892329 MS.gene03094:CDS
AGTGGCCACCGACAATAGAG+CGG 0.719424 2.3:-68891395 MS.gene03094:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTGACAAAAAAAATAAA+AGG + chr2.3:68892222-68892241 None:intergenic 10.0%
!!! TTATTCAATAATTTTTTAGA+TGG - chr2.3:68891166-68891185 MS.gene03094:intron 10.0%
!!! TTATTTTTCTTTTGAAAATT+GGG - chr2.3:68892116-68892135 MS.gene03094:intron 10.0%
!!! TTTATTTTTCTTTTGAAAAT+TGG - chr2.3:68892115-68892134 MS.gene03094:intron 10.0%
!!! TTTTTTTAAGTATATGAAAT+TGG + chr2.3:68890589-68890608 None:intergenic 10.0%
!! GAGTATTTGTTTATATATAA+GGG + chr2.3:68891788-68891807 None:intergenic 15.0%
!! TAAAGAGTAAAATACTTTAA+AGG - chr2.3:68890642-68890661 MS.gene03094:intron 15.0%
!!! AGAGTATTTGTTTATATATA+AGG + chr2.3:68891789-68891808 None:intergenic 15.0%
!!! ATCTTTGTTATTAAAGTATT+AGG + chr2.3:68890558-68890577 None:intergenic 15.0%
!!! GAATTTAATCTCTAAAATAT+CGG - chr2.3:68890456-68890475 MS.gene03094:CDS 15.0%
!!! TACTTTAAAGGTTAAATTAA+CGG - chr2.3:68890654-68890673 MS.gene03094:intron 15.0%
!! AAGAAAATATGATTGCTTTA+AGG + chr2.3:68891253-68891272 None:intergenic 20.0%
!! AATGTATCTGATTCAAAATT+TGG - chr2.3:68891050-68891069 MS.gene03094:intron 20.0%
!! ACATGTTAATGAATATAATG+TGG - chr2.3:68892086-68892105 MS.gene03094:intron 20.0%
!! ATAAACAAATACTCTTGTAT+TGG - chr2.3:68891794-68891813 MS.gene03094:intron 20.0%
!! CCATATTATGAATTCAAAAA+CGG + chr2.3:68891420-68891439 None:intergenic 20.0%
!! GAATCAATGTTGAAATAAAA+TGG - chr2.3:68890705-68890724 MS.gene03094:intron 20.0%
!! GTACAATTAAGTATACTATT+TGG + chr2.3:68891503-68891522 None:intergenic 20.0%
!! TATATAATATCTCAAGTTCT+TGG - chr2.3:68891583-68891602 MS.gene03094:intron 20.0%
!! TTTAACATTTACTCTCTTAT+TGG + chr2.3:68892484-68892503 None:intergenic 20.0%
!! TTTGGATAATATGCAAATAA+TGG + chr2.3:68891485-68891504 None:intergenic 20.0%
!!! CTGATATGTGATTTTAAATA+TGG - chr2.3:68890683-68890702 MS.gene03094:intron 20.0%
!!! GAATATAGTTTTAAAGAACT+CGG + chr2.3:68890987-68891006 None:intergenic 20.0%
! AAATGACATAATATAGAGCA+TGG + chr2.3:68891380-68891399 None:intergenic 25.0%
! ACTTGAGATATTATATAAGC+CGG + chr2.3:68891580-68891599 None:intergenic 25.0%
! ATAAAGAGTTTAGAGGAATA+TGG - chr2.3:68892724-68892743 MS.gene03094:intron 25.0%
! ATTGTTCATAAAGAGTTTAG+AGG - chr2.3:68892717-68892736 MS.gene03094:intron 25.0%
! TAAAGAGGACATTAGAAAAA+AGG - chr2.3:68892500-68892519 MS.gene03094:intron 25.0%
! TAAGAGAGTAAATGTTAAAG+AGG - chr2.3:68892485-68892504 MS.gene03094:intron 25.0%
! TAATCTTATTGAAGAACTCT+CGG + chr2.3:68892569-68892588 None:intergenic 25.0%
! TAGAGGAATATGGATATATT+TGG - chr2.3:68892734-68892753 MS.gene03094:intron 25.0%
! TGTATTATGTTGAATGTAGT+TGG - chr2.3:68890735-68890754 MS.gene03094:intron 25.0%
! TTACATTCAAAACCATTTCA+TGG + chr2.3:68890312-68890331 None:intergenic 25.0%
! TTGTTTATATATAAGGGCAA+AGG + chr2.3:68891782-68891801 None:intergenic 25.0%
!! AATCCTATATGTTTGTTAAC+AGG - chr2.3:68892646-68892665 MS.gene03094:intron 25.0%
!! GAATATACACAACTTTTCAA+CGG + chr2.3:68891846-68891865 None:intergenic 25.0%
!! GCAAATAATGGAAAAAGTTT+AGG + chr2.3:68891473-68891492 None:intergenic 25.0%
!! TTCATTAACATGTGTTTCTA+AGG + chr2.3:68892079-68892098 None:intergenic 25.0%
!! TTGAGGTTTGATTTATAGTT+AGG + chr2.3:68892319-68892338 None:intergenic 25.0%
!!! CAATAATTTTTTAGATGGTG+TGG - chr2.3:68891171-68891190 MS.gene03094:intron 25.0%
!!! CCGTTTTTGAATTCATAATA+TGG - chr2.3:68891417-68891436 MS.gene03094:CDS 25.0%
!!! GAAATGGTTTTGAATGTAAA+TGG - chr2.3:68890313-68890332 MS.gene03094:CDS 25.0%
!!! TTCTCAATATGTTTAGTTTG+AGG + chr2.3:68892336-68892355 None:intergenic 25.0%
AAAACGGTTGCATACATATA+CGG + chr2.3:68891404-68891423 None:intergenic 30.0%
AACAATCACTCTCTTATTGA+GGG - chr2.3:68892390-68892409 MS.gene03094:CDS 30.0%
AACTCTTTATGAACAATAGC+AGG + chr2.3:68892714-68892733 None:intergenic 30.0%
AATCCATATGCTTCAAAGTT+GGG + chr2.3:68891100-68891119 None:intergenic 30.0%
AATTGCAAATATGTCACAAG+TGG - chr2.3:68891961-68891980 MS.gene03094:intron 30.0%
ACACTTAGGCTTATATTCAA+TGG - chr2.3:68891625-68891644 MS.gene03094:intron 30.0%
CAAAAAAGTTCTATGTAGAG+TGG + chr2.3:68890416-68890435 None:intergenic 30.0%
CATTCCTCAAATATAGTTAG+TGG - chr2.3:68892532-68892551 MS.gene03094:intron 30.0%
CTTCTAAATGTTATGTTTGC+CGG - chr2.3:68892286-68892305 MS.gene03094:CDS 30.0%
GCTTTATATGCGAAAAAGTT+GGG - chr2.3:68891015-68891034 MS.gene03094:intron 30.0%
GGAAGAGAAATTTCAATTGA+AGG + chr2.3:68891452-68891471 None:intergenic 30.0%
TAACAATCACTCTCTTATTG+AGG - chr2.3:68892389-68892408 MS.gene03094:CDS 30.0%
TAATCCATATGCTTCAAAGT+TGG + chr2.3:68891101-68891120 None:intergenic 30.0%
TATCTTATGTTCGTCATTTC+TGG - chr2.3:68891727-68891746 MS.gene03094:intron 30.0%
TATTTGGTATCAACAACTCT+TGG - chr2.3:68892750-68892769 MS.gene03094:intron 30.0%
TCTTCAATCTATAATCTTGC+AGG - chr2.3:68891552-68891571 MS.gene03094:CDS 30.0%
TGCTTTATATGCGAAAAAGT+TGG - chr2.3:68891014-68891033 MS.gene03094:intron 30.0%
TTATTGGATCAAACTCACAT+GGG + chr2.3:68892468-68892487 None:intergenic 30.0%
TTCCTTAAAGGAGTAGAAAA+TGG - chr2.3:68892852-68892871 MS.gene03094:CDS 30.0%
TTCTAAATGTTATGTTTGCC+GGG - chr2.3:68892287-68892306 MS.gene03094:CDS 30.0%
TTTATAGTTAGGTTCATACC+CGG + chr2.3:68892308-68892327 None:intergenic 30.0%
! ACAATAGTGTTGAAGATTGA+TGG + chr2.3:68892017-68892036 None:intergenic 30.0%
! TTTTTGTCAATATCGCAGAT+TGG - chr2.3:68892229-68892248 MS.gene03094:CDS 30.0%
AAATATGTCACAAGTGGACA+TGG - chr2.3:68891967-68891986 MS.gene03094:intron 35.0%
AAGCAATGGAAACCATGAAA+TGG - chr2.3:68890297-68890316 MS.gene03094:CDS 35.0%
AATATGTCACAAGTGGACAT+GGG - chr2.3:68891968-68891987 MS.gene03094:intron 35.0%
ACGTACTTCTTGATATTTGC+AGG - chr2.3:68890775-68890794 MS.gene03094:intron 35.0%
AGTTCTATGTAGAGTGGTTT+AGG + chr2.3:68890410-68890429 None:intergenic 35.0%
ATCCTGGGATTTCAATTGTT+TGG - chr2.3:68890845-68890864 MS.gene03094:intron 35.0%
ATCTATAATCTTGCAGGTAC+CGG - chr2.3:68891558-68891577 MS.gene03094:CDS 35.0%
CGTACTTCTTGATATTTGCA+GGG - chr2.3:68890776-68890795 MS.gene03094:intron 35.0%
CTTAAAGGAGTAGAAAATGG+TGG - chr2.3:68892855-68892874 MS.gene03094:CDS 35.0%
CTTATTGGATCAAACTCACA+TGG + chr2.3:68892469-68892488 None:intergenic 35.0%
GTGTCCACTAACTATATTTG+AGG + chr2.3:68892539-68892558 None:intergenic 35.0%
TAGGCTTATATTCAATGGCA+AGG - chr2.3:68891630-68891649 MS.gene03094:intron 35.0%
TCCTGGGATTTCAATTGTTT+GGG - chr2.3:68890846-68890865 MS.gene03094:intron 35.0%
TGGCCTGTTAACAAACATAT+AGG + chr2.3:68892652-68892671 None:intergenic 35.0%
TTACCACTTTGTCATTGTCA+AGG + chr2.3:68890822-68890841 None:intergenic 35.0%
TTCATTGATGCTCCAGTAAT+TGG + chr2.3:68892672-68892691 None:intergenic 35.0%
TTTGTTAACAGGCCAATTAC+TGG - chr2.3:68892657-68892676 MS.gene03094:intron 35.0%
! CACCATTTTCTACTCCTTTA+AGG + chr2.3:68892857-68892876 None:intergenic 35.0%
! CATATTAACACGTGTCGTTA+AGG + chr2.3:68892198-68892217 None:intergenic 35.0%
! TTCTACTCCTTTAAGGAAAG+AGG + chr2.3:68892850-68892869 None:intergenic 35.0%
!!! ATGGTTTTGGTTTACTCTGT+TGG - chr2.3:68890880-68890899 MS.gene03094:CDS 35.0%
AATATCGCAGATTGGTGAGT+TGG - chr2.3:68892237-68892256 MS.gene03094:CDS 40.0%
ACAAAGTGGTAACACATCCT+GGG - chr2.3:68890830-68890849 MS.gene03094:intron 40.0%
ATACACACCTTCTGCAAAAG+AGG + chr2.3:68890385-68890404 None:intergenic 40.0%
CATGGGCATATACTTTCATC+CGG - chr2.3:68892787-68892806 MS.gene03094:CDS 40.0%
CATGTCGTCACAATTCAGTT+TGG - chr2.3:68892593-68892612 MS.gene03094:intron 40.0%
CCTGGGATTTCAATTGTTTG+GGG - chr2.3:68890847-68890866 MS.gene03094:intron 40.0%
CTCTTACTAATTTCACGCAC+TGG + chr2.3:68892825-68892844 None:intergenic 40.0%
GAACCTTGACAATGACAAAG+TGG - chr2.3:68890816-68890835 MS.gene03094:intron 40.0%
GAATTGGGTATCACCTAAAG+TGG + chr2.3:68892433-68892452 None:intergenic 40.0%
GATGTTATGCGTGACTATGA+TGG - chr2.3:68891136-68891155 MS.gene03094:intron 40.0%
GCTGATCATTCATCAAGCAA+TGG - chr2.3:68890283-68890302 MS.gene03094:CDS 40.0%
TACTCTGTTGGTCACATTTC+TGG - chr2.3:68890892-68890911 MS.gene03094:CDS 40.0%
TCTTATTGAGGGAACTCATG+TGG - chr2.3:68892401-68892420 MS.gene03094:intron 40.0%
TGAATTCAACCACAAATGCC+TGG + chr2.3:68891696-68891715 None:intergenic 40.0%
TTCGCATATAAAGCACACGA+CGG + chr2.3:68891009-68891028 None:intergenic 40.0%
! GCATGACTCATCACACTTTT+GGG + chr2.3:68890347-68890366 None:intergenic 40.0%
! TATGTTCGTCATTTCTGGAG+TGG - chr2.3:68891732-68891751 MS.gene03094:intron 40.0%
! TGCATGACTCATCACACTTT+TGG + chr2.3:68890348-68890367 None:intergenic 40.0%
!! AAGAGTGCCTCTTTCCTTAA+AGG - chr2.3:68892840-68892859 MS.gene03094:CDS 40.0%
!! CAGGAAGTGTTCCTGAAAAA+TGG + chr2.3:68891660-68891679 None:intergenic 40.0%
!! GGCAAGGAAAACCATTTTTC+AGG - chr2.3:68891646-68891665 MS.gene03094:intron 40.0%
!! TGACCCAACTTTGAAGCATA+TGG - chr2.3:68891094-68891113 MS.gene03094:intron 40.0%
!! TTGAATGTAGTTGGTAGCAG+AGG - chr2.3:68890744-68890763 MS.gene03094:intron 40.0%
AGTAGATCCAACTGCAAGTC+CGG + chr2.3:68892263-68892282 None:intergenic 45.0%
ATATGCCCATGTACCAGCTA+TGG + chr2.3:68892778-68892797 None:intergenic 45.0%
CAAAAGGTTTCCGGTTAAGC+AGG - chr2.3:68890957-68890976 MS.gene03094:intron 45.0%
CATGTGGATCACACCACTTT+AGG - chr2.3:68892417-68892436 MS.gene03094:intron 45.0%
CCCCAAACAATTGAAATCCC+AGG + chr2.3:68890850-68890869 None:intergenic 45.0%
CTCTTGCAAGTGGAACACTT+AGG - chr2.3:68891611-68891630 MS.gene03094:intron 45.0%
CTTGGATCAACTCTTGCAAG+TGG - chr2.3:68891601-68891620 MS.gene03094:intron 45.0%
GACAAAGTGGTAACACATCC+TGG - chr2.3:68890829-68890848 MS.gene03094:intron 45.0%
GGAGATTACCGCTCTATTGT+CGG + chr2.3:68891761-68891780 None:intergenic 45.0%
TGCATCCACCAAAAGGTTTC+CGG - chr2.3:68890948-68890967 MS.gene03094:intron 45.0%
TTCTGATCTCCAGGCATTTG+TGG - chr2.3:68891684-68891703 MS.gene03094:intron 45.0%
TTGCTCATGCATCCACCAAA+AGG - chr2.3:68890941-68890960 MS.gene03094:intron 45.0%
! CTATGTGCCTCTTTTGCAGA+AGG - chr2.3:68890375-68890394 MS.gene03094:CDS 45.0%
! CTGCTTAACCGGAAACCTTT+TGG + chr2.3:68890959-68890978 None:intergenic 45.0%
! CTTAACCGGAAACCTTTTGG+TGG + chr2.3:68890956-68890975 None:intergenic 45.0%
! GCAAAAGAGGCACATAGTCT+TGG + chr2.3:68890372-68890391 None:intergenic 45.0%
! GGGTCTCACTGTTATGGTTT+TGG - chr2.3:68890867-68890886 MS.gene03094:CDS 45.0%
! TGTTTGGGGTCTCACTGTTA+TGG - chr2.3:68890861-68890880 MS.gene03094:CDS 45.0%
! TTTGGTGGATGCATGAGCAA+TGG + chr2.3:68890941-68890960 None:intergenic 45.0%
!! GGGTCTCACACTTTGGAATT+GGG + chr2.3:68892448-68892467 None:intergenic 45.0%
!! TAGTTGGTAGCAGAGGTTGT+GGG - chr2.3:68890751-68890770 MS.gene03094:intron 45.0%
!! TGGGTCTCACACTTTGGAAT+TGG + chr2.3:68892449-68892468 None:intergenic 45.0%
!! TTTTCAGGAACACTTCCTGC+TGG - chr2.3:68891661-68891680 MS.gene03094:intron 45.0%
ACTCGGATCACCTGCTTAAC+CGG + chr2.3:68890970-68890989 None:intergenic 50.0%
ATGAACCCTGCAAGGAGCTT+AGG - chr2.3:68892687-68892706 MS.gene03094:intron 50.0%
CTCACATGGGTCTCACACTT+TGG + chr2.3:68892455-68892474 None:intergenic 50.0%
GAGCATCAATGAACCCTGCA+AGG - chr2.3:68892679-68892698 MS.gene03094:intron 50.0%
GATTACCGCTCTATTGTCGG+TGG + chr2.3:68891758-68891777 None:intergenic 50.0%
!! ACAACTCTTGGTGCCATAGC+TGG - chr2.3:68892762-68892781 MS.gene03094:intron 50.0%
!! AGTTGGTAGCAGAGGTTGTG+GGG - chr2.3:68890752-68890771 MS.gene03094:intron 50.0%
!! GCACTGGCTTGTTTGTGTAC+CGG + chr2.3:68892809-68892828 None:intergenic 50.0%
!! GTAGTTGGTAGCAGAGGTTG+TGG - chr2.3:68890750-68890769 MS.gene03094:intron 50.0%
!! TGGTGCCATAGCTGGTACAT+GGG - chr2.3:68892770-68892789 MS.gene03094:intron 50.0%
!! TTGGTGCCATAGCTGGTACA+TGG - chr2.3:68892769-68892788 MS.gene03094:intron 50.0%
AGTGGCCACCGACAATAGAG+CGG - chr2.3:68891750-68891769 MS.gene03094:intron 55.0%
CAGGTCCTAAGCTCCTTGCA+GGG + chr2.3:68892695-68892714 None:intergenic 55.0%
GCATGAGCAATGGTGACAGC+AGG + chr2.3:68890931-68890950 None:intergenic 55.0%
! TCGCAGATTGGTGAGTTGGC+CGG - chr2.3:68892241-68892260 MS.gene03094:CDS 55.0%
!! TCCTGCTGGTTCTGATCTCC+AGG - chr2.3:68891675-68891694 MS.gene03094:intron 55.0%
GAGTTGGCCGGACTTGCAGT+TGG - chr2.3:68892253-68892272 MS.gene03094:CDS 60.0%
GCAGGTCCTAAGCTCCTTGC+AGG + chr2.3:68892696-68892715 None:intergenic 60.0%
GCCTGGAGATCAGAACCAGC+AGG + chr2.3:68891679-68891698 None:intergenic 60.0%
! TGCAGGGTGTGCTGCTGTTG+TGG - chr2.3:68890792-68890811 MS.gene03094:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 68890280 68892887 68890280 ID=MS.gene03094
chr2.3 mRNA 68890280 68892887 68890280 ID=MS.gene03094.t1;Parent=MS.gene03094
chr2.3 exon 68892771 68892887 68892771 ID=MS.gene03094.t1.exon1;Parent=MS.gene03094.t1
chr2.3 CDS 68892771 68892887 68892771 ID=cds.MS.gene03094.t1;Parent=MS.gene03094.t1
chr2.3 exon 68892189 68892413 68892189 ID=MS.gene03094.t1.exon2;Parent=MS.gene03094.t1
chr2.3 CDS 68892189 68892413 68892189 ID=cds.MS.gene03094.t1;Parent=MS.gene03094.t1
chr2.3 exon 68891396 68891593 68891396 ID=MS.gene03094.t1.exon3;Parent=MS.gene03094.t1
chr2.3 CDS 68891396 68891593 68891396 ID=cds.MS.gene03094.t1;Parent=MS.gene03094.t1
chr2.3 exon 68890859 68890920 68890859 ID=MS.gene03094.t1.exon4;Parent=MS.gene03094.t1
chr2.3 CDS 68890859 68890920 68890859 ID=cds.MS.gene03094.t1;Parent=MS.gene03094.t1
chr2.3 exon 68890280 68890499 68890280 ID=MS.gene03094.t1.exon5;Parent=MS.gene03094.t1
chr2.3 CDS 68890280 68890499 68890280 ID=cds.MS.gene03094.t1;Parent=MS.gene03094.t1
Gene Sequence

>MS.gene03094

ATGGCTGATCATTCATCAAGCAATGGAAACCATGAAATGGTTTTGAATGTAAATGGTGATGCTTCCCAAAAGTGTGATGAGTCATGCATCCAAGACTATGTGCCTCTTTTGCAGAAGGTGTGTATCTCCTAAACCACTCTACATAGAACTTTTTTGTTGATAATAACTATGAATATGAATTTAATCTCTAAAATATCGGTCTCTAATTTTATTTTTGAAGTAAATAAAATTCGTTGTCATAGTCTCTATAGTATATGAAAGTTATTATATCAATTCCTAATACTTTAATAACAAAGATTAAAGTGACCAATTTCATATACTTAAAAAAAGTTTATTACTTTTATATGCTTTAAAGATTTAATTAAAGAGTAAAATACTTTAAAGGTTAAATTAACGGTTTATTCTGATATGTGATTTTAAATATGGAATCAATGTTGAAATAAAATGGTTTGATTTGTATTATGTTGAATGTAGTTGGTAGCAGAGGTTGTGGGGACGTACTTCTTGATATTTGCAGGGTGTGCTGCTGTTGTGGTGAACCTTGACAATGACAAAGTGGTAACACATCCTGGGATTTCAATTGTTTGGGGTCTCACTGTTATGGTTTTGGTTTACTCTGTTGGTCACATTTCTGGTGCTCATTTCAATCCTGCTGTCACCATTGCTCATGCATCCACCAAAAGGTTTCCGGTTAAGCAGGTGATCCGAGTTCTTTAAAACTATATTCCGTCGTGTGCTTTATATGCGAAAAAGTTGGGTCAACAAAAATTAATGTATCTGATTCAAAATTTGGATCAAATACATAAATTTTTACTGACCCAACTTTGAAGCATATGGATTATTTTATTTTGAAAACGATGTTATGCGTGACTATGATGGTACACATTTATTCAATAATTTTTTAGATGGTGTGGTAAATGAAAATATAATTATTACACTTGAATTTTCTTGAAAATTACTTGTGTTAGCTCCTTAAAGCAATCATATTTTCTTTAAAGAAAATGTTAACACATGCTCTAAAAGCACTTGATAAGAAGTTTAAAGTAGAATTTTTTTTCTATAAGATTGATTTTACATATAAAAAAGCAAAAGCTAAACCATGCTCTATATTATGTCATTTTCCGTATATGTATGCAACCGTTTTTGAATTCATAATATGGTTGAATTGACCTTCAATTGAAATTTCTCTTCCTAAACTTTTTCCATTATTTGCATATTATCCAAATAGTATACTTAATTGTACTAGTTTGTCTAAAAAAATTGTTGAGTTTTTCTTCAATCTATAATCTTGCAGGTACCGGCTTATATAATATCTCAAGTTCTTGGATCAACTCTTGCAAGTGGAACACTTAGGCTTATATTCAATGGCAAGGAAAACCATTTTTCAGGAACACTTCCTGCTGGTTCTGATCTCCAGGCATTTGTGGTTGAATTCATAATCACTTTTTATCTTATGTTCGTCATTTCTGGAGTGGCCACCGACAATAGAGCGGTAATCTCCTTTGCCCTTATATATAAACAAATACTCTTGTATTGGAAAGTGTTGAAATATTGTATATTCAACCGTTGAAAAGTTGTGTATATTCAACTTTTTGAAAATAAAAAATTTGTTATTTTCAATGAAAAGTTTTTTACTTTCTTATATTCTTAACTAATTAGCATGACTCTTAAAATATGTATAAATTGCAAATATGTCACAAGTGGACATGGGCAGCAAATTCTACATTTCAATGTCCATCAATCTTCAACACTATTGTATACTAGATTTTGTTATTTTAGTGAAATGCAAACATGCGCCTTAGAAACACATGTTAATGAATATAATGTGGATATTTTTTATTTTTCTTTTGAAAATTGGGCATTTAATTTTTTAAATATTTAAAAGTTGTTTTCTGCAATGTAAATGTTCTACATGCCTTAACGACACGTGTTAATATGACCTTTTATTTTTTTTGTCAATATCGCAGATTGGTGAGTTGGCCGGACTTGCAGTTGGATCTACTGTGCTTCTAAATGTTATGTTTGCCGGGTATGAACCTAACTATAAATCAAACCTCAAACTAAACATATTGAGAAATGCTAGAAACATTCGCTCTCTAACACTATTTTTAACAATCACTCTCTTATTGAGGGAACTCATGTGGATCACACCACTTTAGGTGATACCCAATTCCAAAGTGTGAGACCCATGTGAGTTTGATCCAATAAGAGAGTAAATGTTAAAGAGGACATTAGAAAAAAGGTGCTCAGATCATTCCTCAAATATAGTTAGTGGACACTTTTACACCGAGAGTTCTTCAATAAGATTACAATCATGTCGTCACAATTCAGTTTGGTCAAATTACTTAGAACTTAATCATGCATGCAATCCTATATGTTTGTTAACAGGCCAATTACTGGAGCATCAATGAACCCTGCAAGGAGCTTAGGACCTGCTATTGTTCATAAAGAGTTTAGAGGAATATGGATATATTTGGTATCAACAACTCTTGGTGCCATAGCTGGTACATGGGCATATACTTTCATCCGGTACACAAACAAGCCAGTGCGTGAAATTAGTAAGAGTGCCTCTTTCCTTAAAGGAGTAGAAAATGGTGGAGCTAAGTGA
Protein sequence

>MS.gene03094.t1

MADHSSSNGNHEMVLNVNGDASQKCDESCIQDYVPLLQKLVAEVVGTYFLIFAGCAAVVVNLDNDKVVTHPGISIVWGLTVMVLVYSVGHISGAHFNPAVTIAHASTKRFPVKQVPAYIISQVLGSTLASGTLRLIFNGKENHFSGTLPAGSDLQAFVVEFIITFYLMFVISGVATDNRAIGELAGLAVGSTVLLNVMFAGPITGASMNPARSLGPAIVHKEFRGIWIYLVSTTLGAIAGTWAYTFIRYTNKPVREISKSASFLKGVENGGAK