AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene39955


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39955.t1 MTR_1g071630 91.304 161 8 1 1 155 5 165 9.86e-84 243
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39955.t1 AT4G04614 56.589 129 51 3 28 155 52 176 1.16e-41 137
MS.gene39955.t1 AT4G04614 56.589 129 51 3 28 155 52 176 1.16e-41 137

Find 35 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTTTGTGTCATCTTCTT+TGG 0.118453 1.4:+58582706 None:intergenic
TTCTTTGTGTCATCTTCTTT+GGG 0.233840 1.4:+58582707 None:intergenic
AGGGACAATGGAGTTTATTC+TGG 0.281268 1.4:-58582452 MS.gene39955:CDS
CTGAAGCGATACGGCTATAA+AGG 0.293708 1.4:-58582758 MS.gene39955:CDS
TAAATCTACAAAGCAGAAAA+AGG 0.315518 1.4:-58582801 MS.gene39955:CDS
AGGCGGACCTTCAGTCTTAA+AGG 0.331123 1.4:-58582738 MS.gene39955:CDS
TCTGAAAATGATAGTGAAAC+TGG 0.358513 1.4:-58583902 MS.gene39955:CDS
CTCAGAAGGAAAAGAAGAAA+AGG 0.400791 1.4:-58582532 MS.gene39955:CDS
GGGATCTTGGTCAAGCAAGC+AGG 0.419342 1.4:-58582511 MS.gene39955:CDS
GAAAAGAAGAAAAGGGATCT+TGG 0.429119 1.4:-58582524 MS.gene39955:CDS
TTCAGAGCTTCAAAGTCAAT+TGG 0.442566 1.4:+58582776 None:intergenic
AAGATGACACAAAGAAAGAT+TGG 0.453949 1.4:-58582700 MS.gene39955:CDS
GTGTCATCTTCTTTGGGTGG+AGG 0.460313 1.4:+58582713 None:intergenic
CAGTTGGCATTTGCTCTCCT+AGG 0.493250 1.4:+58582591 None:intergenic
ATTATGTTGAAGAAGAGAAA+AGG 0.499802 1.4:-58582481 MS.gene39955:CDS
GGATCTTGGTCAAGCAAGCA+GGG 0.515240 1.4:-58582510 MS.gene39955:CDS
TTTGTGTCATCTTCTTTGGG+TGG 0.516808 1.4:+58582710 None:intergenic
TCAGAAGGAAAAGAAGAAAA+GGG 0.533332 1.4:-58582531 MS.gene39955:CDS
AGAAGAGAAAAGGCTGTTGA+GGG 0.535745 1.4:-58582471 MS.gene39955:CDS
TCATTCCTAGTCAGAACAGT+TGG 0.556209 1.4:+58582575 None:intergenic
ACACAAAGAAAGATTGGTCC+TGG 0.556354 1.4:-58582694 MS.gene39955:CDS
ACAAGAGAGGCTCTTTCCCT+AGG 0.557271 1.4:-58582608 MS.gene39955:CDS
AAAAGGCTGTTGAGGGACAA+TGG 0.563867 1.4:-58582464 MS.gene39955:CDS
TTTGAAGCTCTGAAGCGATA+CGG 0.565621 1.4:-58582767 MS.gene39955:CDS
TTGGTCCTGGTCCAGTGGAA+AGG 0.567873 1.4:-58582681 MS.gene39955:CDS
AGTTGGCATTTGCTCTCCTA+GGG 0.578927 1.4:+58582592 None:intergenic
AAGAAGAGAAAAGGCTGTTG+AGG 0.580825 1.4:-58582472 MS.gene39955:CDS
AAAGATTGGTCCTGGTCCAG+TGG 0.594354 1.4:-58582686 MS.gene39955:CDS
GGAGGTACCTTTAAGACTGA+AGG 0.597747 1.4:+58582731 None:intergenic
AAATGCCAACTGTTCTGACT+AGG 0.603014 1.4:-58582580 MS.gene39955:CDS
CTATGAAGAGGACAATGCCA+TGG 0.617111 1.4:-58583958 None:intergenic
ATCTTGGTCAAGCAAGCAGG+GGG 0.627378 1.4:-58582508 MS.gene39955:CDS
GATCTTGGTCAAGCAAGCAG+GGG 0.638659 1.4:-58582509 MS.gene39955:CDS
AGAGAGAAAGAAAACAAGAG+AGG 0.657420 1.4:-58582621 MS.gene39955:CDS
AAGCGATACGGCTATAAAGG+CGG 0.718806 1.4:-58582755 MS.gene39955:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATGTAAATTAGAATATCG+GGG - chr1.4:58583341-58583360 MS.gene39955:intron 20.0%
!! AAGAACTATTATAGCATTAT+CGG + chr1.4:58583254-58583273 None:intergenic 20.0%
!! AGACAATAATATATTGCTAA+CGG + chr1.4:58583378-58583397 None:intergenic 20.0%
!! ATAATCACATTAAGAAAGAA+CGG + chr1.4:58582833-58582852 None:intergenic 20.0%
!! ATATAATTCAACGTAGTTAT+AGG + chr1.4:58582543-58582562 None:intergenic 20.0%
!! ATATTCTAATTTACATTTCC+TGG + chr1.4:58583339-58583358 None:intergenic 20.0%
!! GAAATCTAATGTGATAAATT+AGG - chr1.4:58582986-58583005 MS.gene39955:intron 20.0%
!! GAAATGTAAATTAGAATATC+GGG - chr1.4:58583340-58583359 MS.gene39955:intron 20.0%
!! GGAAATGTAAATTAGAATAT+CGG - chr1.4:58583339-58583358 MS.gene39955:intron 20.0%
!! TGATAAGTGAAAATAGTATT+TGG - chr1.4:58582770-58582789 MS.gene39955:CDS 20.0%
!!! ATTGTTTTAGATTTTGTTGT+TGG - chr1.4:58583136-58583155 MS.gene39955:intron 20.0%
!!! TAAAATACCTTTGGATTTTT+TGG + chr1.4:58582509-58582528 None:intergenic 20.0%
!!! TTGTTTTAGATTTTGTTGTT+GGG - chr1.4:58583137-58583156 MS.gene39955:intron 20.0%
! AAAACACAAACACTAATTGT+GGG + chr1.4:58582866-58582885 None:intergenic 25.0%
! AGTGATAGCTGATAATATTA+TGG - chr1.4:58583037-58583056 MS.gene39955:intron 25.0%
! ATTATGTTGAAGAAGAGAAA+AGG - chr1.4:58583902-58583921 MS.gene39955:CDS 25.0%
! CGTTGAATTATATGTTTAAG+TGG - chr1.4:58582550-58582569 MS.gene39955:CDS 25.0%
! GATTTATGAAACACTAACAA+CGG - chr1.4:58582616-58582635 MS.gene39955:CDS 25.0%
! TAAATCTACAAAGCAGAAAA+AGG - chr1.4:58583582-58583601 MS.gene39955:intron 25.0%
!! ACAATTAGTGTTTGTGTTTT+GGG - chr1.4:58582866-58582885 MS.gene39955:intron 25.0%
!! ATTATCTGAAGATCATTTTG+TGG - chr1.4:58582961-58582980 MS.gene39955:intron 25.0%
!!! ACACTTTTAATCTGAAGTAT+CGG - chr1.4:58582733-58582752 MS.gene39955:CDS 25.0%
!!! TGTTTTAGATTTTGTTGTTG+GGG - chr1.4:58583138-58583157 MS.gene39955:intron 25.0%
AAATGTACTTACTTGAACCT+AGG - chr1.4:58582795-58582814 MS.gene39955:CDS 30.0%
AAGATGACACAAAGAAAGAT+TGG - chr1.4:58583683-58583702 MS.gene39955:intron 30.0%
ACCAATAACAAGGAAATACA+AGG + chr1.4:58583105-58583124 None:intergenic 30.0%
ACTAGTAACTAAGCAACATA+TGG + chr1.4:58583539-58583558 None:intergenic 30.0%
ATCACAATACACCAATAACA+AGG + chr1.4:58583115-58583134 None:intergenic 30.0%
ATGTGATAAATTAGGAGTTC+TGG - chr1.4:58582994-58583013 MS.gene39955:intron 30.0%
CAAAACACAAACACTAATTG+TGG + chr1.4:58582867-58582886 None:intergenic 30.0%
CAATAATATATTGCTAACGG+AGG + chr1.4:58583375-58583394 None:intergenic 30.0%
GATACTTCAGATTAAAAGTG+TGG + chr1.4:58582734-58582753 None:intergenic 30.0%
GCTAATTAAGTCACACAATA+TGG + chr1.4:58583409-58583428 None:intergenic 30.0%
TCAGAAGGAAAAGAAGAAAA+GGG - chr1.4:58583852-58583871 MS.gene39955:intron 30.0%
TTCTTTGTGTCATCTTCTTT+GGG + chr1.4:58583679-58583698 None:intergenic 30.0%
TTTCTTTGTGTCATCTTCTT+TGG + chr1.4:58583680-58583699 None:intergenic 30.0%
! AACTAGAGTAGATATTTTCC+AGG - chr1.4:58583318-58583337 MS.gene39955:intron 30.0%
! CACAATTAGTGTTTGTGTTT+TGG - chr1.4:58582865-58582884 MS.gene39955:intron 30.0%
! TCCTTGTATTTCCTTGTTAT+TGG - chr1.4:58583101-58583120 MS.gene39955:intron 30.0%
! TCTGAAAATGATAGTGAAAC+TGG - chr1.4:58582481-58582500 MS.gene39955:CDS 30.0%
!! AAAATCTCATCTCTAGAATG+TGG - chr1.4:58582900-58582919 MS.gene39955:intron 30.0%
AAGGAATCTCTTCTACAATG+GGG + chr1.4:58583086-58583105 None:intergenic 35.0%
AATCTAATCGTGTGTAGTGT+TGG - chr1.4:58582698-58582717 MS.gene39955:CDS 35.0%
ACAAGGAATCTCTTCTACAA+TGG + chr1.4:58583088-58583107 None:intergenic 35.0%
AGAGAGAAAGAAAACAAGAG+AGG - chr1.4:58583762-58583781 MS.gene39955:intron 35.0%
AGTATGAAGCCATCATGATA+CGG + chr1.4:58583467-58583486 None:intergenic 35.0%
CAAGGAATCTCTTCTACAAT+GGG + chr1.4:58583087-58583106 None:intergenic 35.0%
CTCAGAAGGAAAAGAAGAAA+AGG - chr1.4:58583851-58583870 MS.gene39955:intron 35.0%
GAAAAGAAGAAAAGGGATCT+TGG - chr1.4:58583859-58583878 MS.gene39955:intron 35.0%
GATGCACACTAAAATACCTT+TGG + chr1.4:58582518-58582537 None:intergenic 35.0%
TGACACTAACACTTTGATGT+TGG - chr1.4:58582648-58582667 MS.gene39955:CDS 35.0%
TTAACTTACTTCATGTCTGC+AGG - chr1.4:58583559-58583578 MS.gene39955:intron 35.0%
TTCAGAGCTTCAAAGTCAAT+TGG + chr1.4:58583610-58583629 None:intergenic 35.0%
! ATAGCATTATCGGCTGATTT+AGG + chr1.4:58583244-58583263 None:intergenic 35.0%
! GAATCTTTCCTTTTCTCAGA+AGG - chr1.4:58583837-58583856 MS.gene39955:intron 35.0%
! TTCGAGTTTCTTGACTTTCA+AGG - chr1.4:58583294-58583313 MS.gene39955:intron 35.0%
!! CTTCTTTTCCTTCTGAGAAA+AGG + chr1.4:58583848-58583867 None:intergenic 35.0%
!! TTCTGAAATCCGTATCATGA+TGG - chr1.4:58583455-58583474 MS.gene39955:intron 35.0%
AAATGCCAACTGTTCTGACT+AGG - chr1.4:58583803-58583822 MS.gene39955:intron 40.0%
AACTAAGCAACATATGGCAG+TGG + chr1.4:58583533-58583552 None:intergenic 40.0%
AAGAAGAGAAAAGGCTGTTG+AGG - chr1.4:58583911-58583930 MS.gene39955:CDS 40.0%
ACTAACTGAAGGTCAAGATG+GGG + chr1.4:58583173-58583192 None:intergenic 40.0%
ACTGGAGCCAAAAAATCCAA+AGG - chr1.4:58582499-58582518 MS.gene39955:CDS 40.0%
AGAAGAGAAAAGGCTGTTGA+GGG - chr1.4:58583912-58583931 MS.gene39955:CDS 40.0%
AGATGGTTCATAGACAGTTG+TGG - chr1.4:58583201-58583220 MS.gene39955:intron 40.0%
AGGGACAATGGAGTTTATTC+TGG - chr1.4:58583931-58583950 MS.gene39955:CDS 40.0%
CACTAACTGAAGGTCAAGAT+GGG + chr1.4:58583174-58583193 None:intergenic 40.0%
CTTGAACCTAGGATGTTGAT+AGG - chr1.4:58582806-58582825 MS.gene39955:intron 40.0%
TCAGTTAGTGCAATGCAAGA+TGG - chr1.4:58583184-58583203 MS.gene39955:intron 40.0%
TCATCATCATCATCACTCCA+TGG + chr1.4:58582445-58582464 None:intergenic 40.0%
TCATTCCTAGTCAGAACAGT+TGG + chr1.4:58583811-58583830 None:intergenic 40.0%
TCTTGCATTGCACTAACTGA+AGG + chr1.4:58583184-58583203 None:intergenic 40.0%
TTCAGATAATTGCAACGCAG+AGG + chr1.4:58582951-58582970 None:intergenic 40.0%
TTTGTGTCATCTTCTTTGGG+TGG + chr1.4:58583676-58583695 None:intergenic 40.0%
! ACACAAAGAAAGATTGGTCC+TGG - chr1.4:58583689-58583708 MS.gene39955:intron 40.0%
!! TTTGAAGCTCTGAAGCGATA+CGG - chr1.4:58583616-58583635 MS.gene39955:intron 40.0%
AAAAGGCTGTTGAGGGACAA+TGG - chr1.4:58583919-58583938 MS.gene39955:CDS 45.0%
AAAGGAAAAACGCGTCGAGA+AGG - chr1.4:58583720-58583739 MS.gene39955:intron 45.0%
AACGCAGAGGTTTCATACCT+TGG + chr1.4:58582938-58582957 None:intergenic 45.0%
AACGGACCTATCAACATCCT+AGG + chr1.4:58582815-58582834 None:intergenic 45.0%
AAGCGATACGGCTATAAAGG+CGG - chr1.4:58583628-58583647 MS.gene39955:intron 45.0%
AGTTGGCATTTGCTCTCCTA+GGG + chr1.4:58583794-58583813 None:intergenic 45.0%
CTGAAGCGATACGGCTATAA+AGG - chr1.4:58583625-58583644 MS.gene39955:intron 45.0%
GCACTAACTGAAGGTCAAGA+TGG + chr1.4:58583175-58583194 None:intergenic 45.0%
! TTTTTCCTTTCCACTGGACC+AGG + chr1.4:58583710-58583729 None:intergenic 45.0%
!! GGAGGTACCTTTAAGACTGA+AGG + chr1.4:58583655-58583674 None:intergenic 45.0%
!! TCGTGTGTAGTGTTGGTGTA+CGG - chr1.4:58582705-58582724 MS.gene39955:CDS 45.0%
ACAAGAGAGGCTCTTTCCCT+AGG - chr1.4:58583775-58583794 MS.gene39955:intron 50.0%
AGGCGGACCTTCAGTCTTAA+AGG - chr1.4:58583645-58583664 MS.gene39955:intron 50.0%
ATCTTGGTCAAGCAAGCAGG+GGG - chr1.4:58583875-58583894 MS.gene39955:intron 50.0%
CAGTTGGCATTTGCTCTCCT+AGG + chr1.4:58583795-58583814 None:intergenic 50.0%
GATCTTGGTCAAGCAAGCAG+GGG - chr1.4:58583874-58583893 MS.gene39955:intron 50.0%
GGATCTTGGTCAAGCAAGCA+GGG - chr1.4:58583873-58583892 MS.gene39955:intron 50.0%
GTGTCATCTTCTTTGGGTGG+AGG + chr1.4:58583673-58583692 None:intergenic 50.0%
! AAAGATTGGTCCTGGTCCAG+TGG - chr1.4:58583697-58583716 MS.gene39955:intron 50.0%
! GACGCGTTTTTCCTTTCCAC+TGG + chr1.4:58583716-58583735 None:intergenic 50.0%
! TGTGGATTGACGCTCATCCA+AGG - chr1.4:58582918-58582937 MS.gene39955:intron 50.0%
GGGATCTTGGTCAAGCAAGC+AGG - chr1.4:58583872-58583891 MS.gene39955:intron 55.0%
TTGGTCCTGGTCCAGTGGAA+AGG - chr1.4:58583702-58583721 MS.gene39955:intron 55.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 58582439 58583966 58582439 ID=MS.gene39955
chr1.4 mRNA 58582439 58583966 58582439 ID=MS.gene39955.t1;Parent=MS.gene39955
chr1.4 exon 58583885 58583966 58583885 ID=MS.gene39955.t1.exon1;Parent=MS.gene39955.t1
chr1.4 CDS 58583885 58583966 58583885 ID=cds.MS.gene39955.t1;Parent=MS.gene39955.t1
chr1.4 exon 58582439 58582824 58582439 ID=MS.gene39955.t1.exon2;Parent=MS.gene39955.t1
chr1.4 CDS 58582439 58582824 58582439 ID=cds.MS.gene39955.t1;Parent=MS.gene39955.t1
Gene Sequence

>MS.gene39955

ATGCCATGGAGTGATGATGATGATGATGACTCATCTTCAGACTCTGAAAATGATAGTGAAACTGGAGCCAAAAAATCCAAAGGTATTTTAGTGTGCATCATCCTATAACTACGTTGAATTATATGTTTAAGTGGAGTATTTTTTGAGTATTTTGTTGACTACAATGTATTCATGTGTGATTTATGAAACACTAACAACGGACATGAACATGACACTAACACTTTGATGTTGGTACTAATTTGAGAAAATAAATTAATTGAATCTAATCGTGTGTAGTGTTGGTGTACGGACACCACACTTTTAATCTGAAGTATCGGTGCTACAAAGTGTTTGATAAGTGAAAATAGTATTTGGACAAATGTACTTACTTGAACCTAGGATGTTGATAGGTCCGTTCTTTCTTAATGTGATTATAAGTTTATAACCCACAATTAGTGTTTGTGTTTTGGGTCTATTTAATAAAAATCTCATCTCTAGAATGTGGATTGACGCTCATCCAAGGTATGAAACCTCTGCGTTGCAATTATCTGAAGATCATTTTGTGGCTGAAATCTAATGTGATAAATTAGGAGTTCTGGCGAAAACTAAAAGTTAATTGAGTGATAGCTGATAATATTATGGAGAAATTGCACAATTTCATAATGCCCCATTGTAGAAGAGATTCCTTGTATTTCCTTGTTATTGGTGTATTGTGATCATTGTTTTAGATTTTGTTGTTGGGGATATATATACCCCATCTTGACCTTCAGTTAGTGCAATGCAAGATGGTTCATAGACAGTTGTGGTAGATACATAATGATATCCTAAATCAGCCGATAATGCTATAATAGTTCTTTTTATCTTCACAAAAATTCTTTCGAGTTTCTTGACTTTCAAGGCAACTAGAGTAGATATTTTCCAGGAAATGTAAATTAGAATATCGGGGTTGTTATACCTCCGTTAGCAATATATTATTGTCTTTGTTTGACCATATTGTGTGACTTAATTAGCGTTAGTTCATAATTGAAATTGTTAGCTTCTGAAATCCGTATCATGATGGCTTCATACTCATTTGCTTGTAAACTGTGACATCTTGAAAATTCTCACTAATGCCACTGCCATATGTTGCTTAGTTACTAGTTTAACTTACTTCATGTCTGCAGGTAAATCTACAAAGCAGAAAAAGGCTCCAATTGACTTTGAAGCTCTGAAGCGATACGGCTATAAAGGCGGACCTTCAGTCTTAAAGGTACCTCCACCCAAAGAAGATGACACAAAGAAAGATTGGTCCTGGTCCAGTGGAAAGGAAAAACGCGTCGAGAAGGAAGTTGAAGAAACATATGAAGAGAGAAAGAAAACAAGAGAGGCTCTTTCCCTAGGAGAGCAAATGCCAACTGTTCTGACTAGGAATGACAAGAAGAATCTTTCCTTTTCTCAGAAGGAAAAGAAGAAAAGGGATCTTGGTCAAGCAAGCAGGGGGAAAAATTATGTTGAAGAAGAGAAAAGGCTGTTGAGGGACAATGGAGTTTATTCTGGTTTCGATTCTTGA

Protein sequence

>MS.gene39955.t1

MPWSDDDDDDSSSDSENDSETGAKKSKGKSTKQKKAPIDFEALKRYGYKGGPSVLKVPPPKEDDTKKDWSWSSGKEKRVEKEVEETYEERKKTREALSLGEQMPTVLTRNDKKNLSFSQKEKKKRDLGQASRGKNYVEEEKRLLRDNGVYSGFDS