Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42257.t1 | PNX75878.1 | 73.3 | 90 | 24 | 0 | 1 | 90 | 1 | 90 | 2.10E-22 | 114.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42257.t1 | A0A2K3LBG3 | 73.3 | 90 | 24 | 0 | 1 | 90 | 1 | 90 | 1.5e-22 | 114.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049232 | MS.gene42257 | 0.824151 | 9.43E-54 | -1.69E-46 |
| MS.gene049396 | MS.gene42257 | 0.808465 | 3.06E-50 | -1.69E-46 |
| MS.gene050170 | MS.gene42257 | 0.81372 | 2.22E-51 | -1.69E-46 |
| MS.gene050323 | MS.gene42257 | 0.809829 | 1.56E-50 | -1.69E-46 |
| MS.gene050496 | MS.gene42257 | 0.820122 | 8.10E-53 | -1.69E-46 |
| MS.gene050502 | MS.gene42257 | 0.809078 | 2.26E-50 | -1.69E-46 |
| MS.gene050675 | MS.gene42257 | 0.802733 | 4.88E-49 | -1.69E-46 |
| MS.gene050697 | MS.gene42257 | 0.815073 | 1.11E-51 | -1.69E-46 |
| MS.gene050868 | MS.gene42257 | 0.831577 | 1.54E-55 | -1.69E-46 |
| MS.gene050871 | MS.gene42257 | 0.835035 | 2.12E-56 | -1.69E-46 |
| MS.gene051268 | MS.gene42257 | 0.808401 | 3.15E-50 | -1.69E-46 |
| MS.gene051509 | MS.gene42257 | 0.821125 | 4.77E-53 | -1.69E-46 |
| MS.gene051874 | MS.gene42257 | 0.801674 | 8.06E-49 | -1.69E-46 |
| MS.gene051941 | MS.gene42257 | 0.831132 | 1.98E-55 | -1.69E-46 |
| MS.gene052966 | MS.gene42257 | 0.803759 | 2.99E-49 | -1.69E-46 |
| MS.gene053420 | MS.gene42257 | -0.802989 | 4.32E-49 | -1.69E-46 |
| MS.gene053505 | MS.gene42257 | 0.806726 | 7.15E-50 | -1.69E-46 |
| MS.gene053574 | MS.gene42257 | 0.807288 | 5.44E-50 | -1.69E-46 |
| MS.gene053626 | MS.gene42257 | 0.818293 | 2.11E-52 | -1.69E-46 |
| MS.gene053824 | MS.gene42257 | 0.800575 | 1.35E-48 | -1.69E-46 |
| MS.gene054595 | MS.gene42257 | 0.805588 | 1.24E-49 | -1.69E-46 |
| MS.gene055909 | MS.gene42257 | 0.818709 | 1.70E-52 | -1.69E-46 |
| MS.gene055911 | MS.gene42257 | 0.819433 | 1.16E-52 | -1.69E-46 |
| MS.gene056166 | MS.gene42257 | 0.808447 | 3.08E-50 | -1.69E-46 |
| MS.gene056387 | MS.gene42257 | 0.805922 | 1.06E-49 | -1.69E-46 |
| MS.gene056504 | MS.gene42257 | 0.821987 | 3.01E-53 | -1.69E-46 |
| MS.gene057019 | MS.gene42257 | 0.810784 | 9.70E-51 | -1.69E-46 |
| MS.gene057086 | MS.gene42257 | 0.805506 | 1.29E-49 | -1.69E-46 |
| MS.gene05787 | MS.gene42257 | 0.810073 | 1.38E-50 | -1.69E-46 |
| MS.gene058157 | MS.gene42257 | 0.821361 | 4.21E-53 | -1.69E-46 |
| MS.gene058171 | MS.gene42257 | -0.809802 | 1.58E-50 | -1.69E-46 |
| MS.gene058173 | MS.gene42257 | -0.811501 | 6.78E-51 | -1.69E-46 |
| MS.gene058175 | MS.gene42257 | -0.809341 | 1.98E-50 | -1.69E-46 |
| MS.gene05837 | MS.gene42257 | 0.814737 | 1.32E-51 | -1.69E-46 |
| MS.gene059367 | MS.gene42257 | 0.828059 | 1.11E-54 | -1.69E-46 |
| MS.gene059368 | MS.gene42257 | 0.811301 | 7.50E-51 | -1.69E-46 |
| MS.gene059680 | MS.gene42257 | 0.819121 | 1.37E-52 | -1.69E-46 |
| MS.gene059865 | MS.gene42257 | 0.814153 | 1.78E-51 | -1.69E-46 |
| MS.gene06000 | MS.gene42257 | 0.838856 | 2.24E-57 | -1.69E-46 |
| MS.gene060187 | MS.gene42257 | -0.803017 | 4.26E-49 | -1.69E-46 |
| MS.gene060372 | MS.gene42257 | 0.807478 | 4.96E-50 | -1.69E-46 |
| MS.gene060593 | MS.gene42257 | -0.809565 | 1.78E-50 | -1.69E-46 |
| MS.gene060595 | MS.gene42257 | -0.826792 | 2.23E-54 | -1.69E-46 |
| MS.gene060778 | MS.gene42257 | 0.864083 | 1.57E-64 | -1.69E-46 |
| MS.gene06077 | MS.gene42257 | 0.800033 | 1.74E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATAGCAGGGACACATTTCT+TGG | 0.264407 | 3.2:+90327242 | None:intergenic |
| TCTCCATCTTGTTCATGCTT+TGG | 0.293177 | 3.2:-90327942 | MS.gene42257:CDS |
| GGTGGTTTGGTTGGCATAGC+AGG | 0.303830 | 3.2:+90327228 | None:intergenic |
| GGCACTTGCGTCTGCCTCCA+TGG | 0.330551 | 3.2:+90327206 | None:intergenic |
| TGCGTGCCACCAGGAACTTC+TGG | 0.331803 | 3.2:-90327268 | MS.gene42257:CDS |
| CACGGCCGCGCCTCTGCCAC+AGG | 0.395156 | 3.2:-90327321 | MS.gene42257:CDS |
| TCTTGGTTACCAGAAGTTCC+TGG | 0.400394 | 3.2:+90327259 | None:intergenic |
| GTCTGCCTCCATGGGTGGTT+TGG | 0.427775 | 3.2:+90327215 | None:intergenic |
| GCCTCCATGGGTGGTTTGGT+TGG | 0.435015 | 3.2:+90327219 | None:intergenic |
| TTCCACATGCCCTGTGGCAG+AGG | 0.454808 | 3.2:+90327311 | None:intergenic |
| GGCGCGGCCGTGATGATAAA+CGG | 0.465640 | 3.2:+90327332 | None:intergenic |
| CGCCTCTGCCACAGGGCATG+TGG | 0.480960 | 3.2:-90327313 | MS.gene42257:CDS |
| GCACTTGCGTCTGCCTCCAT+GGG | 0.498020 | 3.2:+90327207 | None:intergenic |
| CTTGCGTCTGCCTCCATGGG+TGG | 0.501890 | 3.2:+90327210 | None:intergenic |
| TGATGATTCAACTCAATTCA+AGG | 0.508747 | 3.2:+90327184 | None:intergenic |
| CGGTGCAACTGCGTGCCACC+AGG | 0.530335 | 3.2:-90327277 | MS.gene42257:CDS |
| AGCATGAACAAGATGGAGAA+TGG | 0.540714 | 3.2:+90327946 | None:intergenic |
| GTGGTTTGGTTGGCATAGCA+GGG | 0.546195 | 3.2:+90327229 | None:intergenic |
| TGTTCATGCTTTGGACCAGC+CGG | 0.555470 | 3.2:-90327933 | MS.gene42257:intron |
| GCAATGGAGCATGTGCTGCT+AGG | 0.562822 | 3.2:-90327357 | MS.gene42257:CDS |
| ATATAAATTTCAGATTGCAA+TGG | 0.566444 | 3.2:-90327373 | MS.gene42257:intron |
| GATGATTCAACTCAATTCAA+GGG | 0.567646 | 3.2:+90327185 | None:intergenic |
| GGTCCAAAGCATGAACAAGA+TGG | 0.576917 | 3.2:+90327939 | None:intergenic |
| CATGCCCTGTGGCAGAGGCG+CGG | 0.598879 | 3.2:+90327316 | None:intergenic |
| CATGTGGAACTTGCTGCAGT+CGG | 0.613537 | 3.2:-90327297 | MS.gene42257:CDS |
| ACGGCCGCGCCTCTGCCACA+GGG | 0.616011 | 3.2:-90327320 | MS.gene42257:CDS |
| CTAGGTGCCGTTTATCATCA+CGG | 0.637583 | 3.2:-90327339 | MS.gene42257:CDS |
| TATGCCAACCAAACCACCCA+TGG | 0.640986 | 3.2:-90327223 | MS.gene42257:CDS |
| TGGTTACCAGAAGTTCCTGG+TGG | 0.663688 | 3.2:+90327262 | None:intergenic |
| GCCAACCAAACCACCCATGG+AGG | 0.699460 | 3.2:-90327220 | MS.gene42257:CDS |
| AGCAAGTTCCACATGCCCTG+TGG | 0.700479 | 3.2:+90327305 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATATATGAACTAAACTA+AGG | - | chr3.2:90327533-90327552 | MS.gene42257:intron | 15.0% |
| !! | TGGAAAAAATATAAAAAAAC+TGG | - | chr3.2:90327487-90327506 | MS.gene42257:intron | 15.0% |
| !!! | TTTTAATTATTTGCTTAAAG+TGG | - | chr3.2:90327467-90327486 | MS.gene42257:intron | 15.0% |
| !! | ATATAAATTTCAGATTGCAA+TGG | - | chr3.2:90327810-90327829 | MS.gene42257:intron | 20.0% |
| !! | GGATAAAAGATTAGTAAAAA+AGG | - | chr3.2:90327650-90327669 | MS.gene42257:intron | 20.0% |
| !!! | TATTTTATAAAGAAAACGCT+TGG | + | chr3.2:90327325-90327344 | None:intergenic | 20.0% |
| ! | TAACGAATATGTATGCTAAT+GGG | - | chr3.2:90327629-90327648 | MS.gene42257:intron | 25.0% |
| ! | TTAACGAATATGTATGCTAA+TGG | - | chr3.2:90327628-90327647 | MS.gene42257:intron | 25.0% |
| !! | TTAATTACATGTGTTTTCGT+AGG | - | chr3.2:90327361-90327380 | MS.gene42257:CDS | 25.0% |
| ! | CTACTTCACACACATATTTT+TGG | + | chr3.2:90327778-90327797 | None:intergenic | 30.0% |
| !! | CAACAATAGCTTTGAATTAC+CGG | + | chr3.2:90327272-90327291 | None:intergenic | 30.0% |
| AGCATGAACAAGATGGAGAA+TGG | + | chr3.2:90327240-90327259 | None:intergenic | 40.0% | |
| TAAAGAAAACGCTTGGAGCT+TGG | + | chr3.2:90327318-90327337 | None:intergenic | 40.0% | |
| TCTCCATCTTGTTCATGCTT+TGG | - | chr3.2:90327241-90327260 | MS.gene42257:CDS | 40.0% | |
| ! | GTTTCTTTTCAGCAGCAGAT+AGG | - | chr3.2:90327410-90327429 | MS.gene42257:intron | 40.0% |
| !! | AATAGCTTTGAATTACCGGC+TGG | + | chr3.2:90327268-90327287 | None:intergenic | 40.0% |
| AACCAGACAGAAGCAGTTGA+TGG | + | chr3.2:90327449-90327468 | None:intergenic | 45.0% | |
| CATAGCAGGGACACATTTCT+TGG | + | chr3.2:90327944-90327963 | None:intergenic | 45.0% | |
| GGTCCAAAGCATGAACAAGA+TGG | + | chr3.2:90327247-90327266 | None:intergenic | 45.0% | |
| TCTTGGTTACCAGAAGTTCC+TGG | + | chr3.2:90327927-90327946 | None:intergenic | 45.0% | |
| TGAGTTTCTGCGTGTACATG+CGG | + | chr3.2:90327389-90327408 | None:intergenic | 45.0% | |
| TTCCATCAACTGCTTCTGTC+TGG | - | chr3.2:90327444-90327463 | MS.gene42257:intron | 45.0% | |
| !! | CTAGGTGCCGTTTATCATCA+CGG | - | chr3.2:90327844-90327863 | MS.gene42257:intron | 45.0% |
| !! | GTAATTAAATTAAATTAAAT+TGG | + | chr3.2:90327349-90327368 | None:intergenic | 5.0% |
| TATGCCAACCAAACCACCCA+TGG | - | chr3.2:90327960-90327979 | MS.gene42257:CDS | 50.0% | |
| TGGTTACCAGAAGTTCCTGG+TGG | + | chr3.2:90327924-90327943 | None:intergenic | 50.0% | |
| ! | TGTTCATGCTTTGGACCAGC+CGG | - | chr3.2:90327250-90327269 | MS.gene42257:CDS | 50.0% |
| !! | CATGTGGAACTTGCTGCAGT+CGG | - | chr3.2:90327886-90327905 | MS.gene42257:intron | 50.0% |
| !! | GTGGTTTGGTTGGCATAGCA+GGG | + | chr3.2:90327957-90327976 | None:intergenic | 50.0% |
| AGCAAGTTCCACATGCCCTG+TGG | + | chr3.2:90327881-90327900 | None:intergenic | 55.0% | |
| ! | GCAATGGAGCATGTGCTGCT+AGG | - | chr3.2:90327826-90327845 | MS.gene42257:intron | 55.0% |
| !! | GGTGGTTTGGTTGGCATAGC+AGG | + | chr3.2:90327958-90327977 | None:intergenic | 55.0% |
| GCACTTGCGTCTGCCTCCAT+GGG | + | chr3.2:90327979-90327998 | None:intergenic | 60.0% | |
| GCCAACCAAACCACCCATGG+AGG | - | chr3.2:90327963-90327982 | MS.gene42257:CDS | 60.0% | |
| GGCGCGGCCGTGATGATAAA+CGG | + | chr3.2:90327854-90327873 | None:intergenic | 60.0% | |
| GTCTGCCTCCATGGGTGGTT+TGG | + | chr3.2:90327971-90327990 | None:intergenic | 60.0% | |
| TGCGTGCCACCAGGAACTTC+TGG | - | chr3.2:90327915-90327934 | MS.gene42257:intron | 60.0% | |
| TTCCACATGCCCTGTGGCAG+AGG | + | chr3.2:90327875-90327894 | None:intergenic | 60.0% | |
| !! | GCCTCCATGGGTGGTTTGGT+TGG | + | chr3.2:90327967-90327986 | None:intergenic | 60.0% |
| CTTGCGTCTGCCTCCATGGG+TGG | + | chr3.2:90327976-90327995 | None:intergenic | 65.0% | |
| GGCACTTGCGTCTGCCTCCA+TGG | + | chr3.2:90327980-90327999 | None:intergenic | 65.0% | |
| CATGCCCTGTGGCAGAGGCG+CGG | + | chr3.2:90327870-90327889 | None:intergenic | 70.0% | |
| CGCCTCTGCCACAGGGCATG+TGG | - | chr3.2:90327870-90327889 | MS.gene42257:intron | 70.0% | |
| CGGTGCAACTGCGTGCCACC+AGG | - | chr3.2:90327906-90327925 | MS.gene42257:intron | 70.0% | |
| ACGGCCGCGCCTCTGCCACA+GGG | - | chr3.2:90327863-90327882 | MS.gene42257:intron | 75.0% | |
| ! | CACGGCCGCGCCTCTGCCAC+AGG | - | chr3.2:90327862-90327881 | MS.gene42257:intron | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 90327191 | 90328014 | 90327191 | ID=MS.gene42257 |
| chr3.2 | mRNA | 90327191 | 90328014 | 90327191 | ID=MS.gene42257.t1;Parent=MS.gene42257 |
| chr3.2 | exon | 90327934 | 90328014 | 90327934 | ID=MS.gene42257.t1.exon1;Parent=MS.gene42257.t1 |
| chr3.2 | CDS | 90327934 | 90328014 | 90327934 | ID=cds.MS.gene42257.t1;Parent=MS.gene42257.t1 |
| chr3.2 | exon | 90327191 | 90327382 | 90327191 | ID=MS.gene42257.t1.exon2;Parent=MS.gene42257.t1 |
| chr3.2 | CDS | 90327191 | 90327382 | 90327191 | ID=cds.MS.gene42257.t1;Parent=MS.gene42257.t1 |
| Gene Sequence |
| Protein sequence |
