AlfalfaGEDB | MS.gene42821

M. sativa cultivar XinJiangDaYe / MS.gene42821

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF01344.28 PF13964.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42821.t1	XP_013465289.1	98.9	364	4	0	1	364	1	364	7.20E-178	633.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42821.t1	Q9FKJ0	75.3	348	86	0	17	364	46	393	2.4e-121	436.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42821.t1	A0A072VBD7	98.9	364	4	0	1	364	1	364	5.2e-178	633.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049283	MS.gene42821	0.824952	6.11E-54	-1.69E-46
MS.gene049419	MS.gene42821	-0.812042	5.17E-51	-1.69E-46
MS.gene049801	MS.gene42821	0.809526	1.81E-50	-1.69E-46
MS.gene050084	MS.gene42821	0.81718	3.77E-52	-1.69E-46
MS.gene050085	MS.gene42821	0.823428	1.39E-53	-1.69E-46
MS.gene050359	MS.gene42821	0.837217	5.92E-57	-1.69E-46
MS.gene050361	MS.gene42821	0.840783	7.06E-58	-1.69E-46
MS.gene050414	MS.gene42821	0.806995	6.28E-50	-1.69E-46
MS.gene050646	MS.gene42821	0.829243	5.74E-55	-1.69E-46
MS.gene050654	MS.gene42821	0.846079	2.72E-59	-1.69E-46
MS.gene051122	MS.gene42821	0.814341	1.62E-51	-1.69E-46
MS.gene051184	MS.gene42821	0.807852	4.13E-50	-1.69E-46
MS.gene051209	MS.gene42821	0.808233	3.43E-50	-1.69E-46
MS.gene051275	MS.gene42821	0.818583	1.82E-52	-1.69E-46
MS.gene052675	MS.gene42821	0.80271	4.93E-49	-1.69E-46
MS.gene052677	MS.gene42821	0.801065	1.07E-48	-1.69E-46
MS.gene052954	MS.gene42821	0.801609	8.31E-49	-1.69E-46
MS.gene053058	MS.gene42821	0.801234	9.91E-49	-1.69E-46
MS.gene053726	MS.gene42821	0.843758	1.15E-58	-1.69E-46
MS.gene054479	MS.gene42821	0.802671	5.02E-49	-1.69E-46
MS.gene054892	MS.gene42821	0.813166	2.94E-51	-1.69E-46
MS.gene054893	MS.gene42821	0.824583	7.46E-54	-1.69E-46
MS.gene055864	MS.gene42821	0.81439	1.58E-51	-1.69E-46
MS.gene056097	MS.gene42821	0.802316	5.95E-49	-1.69E-46
MS.gene056480	MS.gene42821	0.831268	1.84E-55	-1.69E-46
MS.gene056787	MS.gene42821	0.803885	2.82E-49	-1.69E-46
MS.gene056849	MS.gene42821	0.802756	4.82E-49	-1.69E-46
MS.gene057371	MS.gene42821	0.809202	2.13E-50	-1.69E-46
MS.gene058292	MS.gene42821	0.804105	2.53E-49	-1.69E-46
MS.gene058429	MS.gene42821	0.815308	9.88E-52	-1.69E-46
MS.gene059007	MS.gene42821	0.877645	5.32E-69	-1.69E-46
MS.gene060850	MS.gene42821	0.829845	4.10E-55	-1.69E-46
MS.gene060851	MS.gene42821	0.826164	3.15E-54	-1.69E-46
MS.gene060852	MS.gene42821	0.825624	4.24E-54	-1.69E-46
MS.gene060933	MS.gene42821	0.801351	9.38E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42821.t1	MTR_2g094093	98.901	364	4	0	1	364	1	364	0.0	744
MS.gene42821.t1	MTR_4g108280	50.276	362	164	5	14	364	24	380	3.14e-123	360
MS.gene42821.t1	MTR_8g468030	50.142	351	162	4	22	364	152	497	1.73e-113	339
MS.gene42821.t1	MTR_5g039390	49.718	354	165	4	19	364	128	476	2.89e-113	338
MS.gene42821.t1	MTR_0345s0010	49.422	346	161	5	27	364	96	435	5.36e-112	333
MS.gene42821.t1	MTR_0105s0170	49.422	346	161	5	27	364	109	448	7.06e-112	333
MS.gene42821.t1	MTR_0105s0020	48.844	346	163	5	27	364	96	435	1.70e-111	332
MS.gene42821.t1	MTR_0105s0130	49.422	346	161	5	27	364	96	435	3.36e-111	332
MS.gene42821.t1	MTR_0105s0040	49.133	346	162	5	27	364	194	533	2.47e-110	332
MS.gene42821.t1	MTR_4g022400	48.295	352	170	4	20	364	68	414	8.85e-110	327
MS.gene42821.t1	MTR_0496s0010	47.977	346	160	6	27	364	78	411	2.78e-109	326
MS.gene42821.t1	MTR_1330s0010	50.789	317	147	3	27	335	109	424	2.99e-108	324
MS.gene42821.t1	MTR_4g022260	47.246	345	170	4	27	364	78	417	3.78e-108	323
MS.gene42821.t1	MTR_7g029290	39.130	345	202	6	24	362	59	401	4.94e-76	240
MS.gene42821.t1	MTR_6g023805	35.775	355	207	6	21	362	57	403	2.38e-70	226
MS.gene42821.t1	MTR_4g114110	30.420	286	177	9	13	286	74	349	2.70e-28	115
MS.gene42821.t1	MTR_8g085600	30.241	291	183	11	8	287	65	346	2.16e-26	109
MS.gene42821.t1	MTR_3g085570	25.610	328	211	9	15	325	30	341	1.54e-21	95.1
MS.gene42821.t1	MTR_8g080370	26.646	319	206	9	17	320	47	352	3.47e-21	94.4
MS.gene42821.t1	MTR_2g019230	26.688	311	205	8	20	315	52	354	3.75e-19	88.6
MS.gene42821.t1	MTR_7g095220	27.245	323	188	13	20	321	4	300	3.43e-18	85.1
MS.gene42821.t1	MTR_2g008440	23.913	368	199	12	14	303	37	401	5.39e-18	85.5
MS.gene42821.t1	MTR_4g011900	24.332	337	205	10	17	319	2	322	1.87e-17	83.2
MS.gene42821.t1	MTR_0105s0060	43.956	91	46	2	274	364	20	105	7.93e-16	73.2
MS.gene42821.t1	MTR_3g073640	26.336	262	164	8	2	243	14	266	8.78e-16	79.0
MS.gene42821.t1	MTR_3g094850	25.788	349	193	13	17	336	6	317	1.93e-14	73.9
MS.gene42821.t1	MTR_8g085650	23.735	257	157	5	20	244	7	256	3.70e-14	73.2
MS.gene42821.t1	MTR_1g043110	60.784	51	19	1	302	351	7	57	7.88e-12	63.2
MS.gene42821.t1	MTR_8g030940	22.939	279	197	8	17	284	22	293	2.35e-11	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42821.t1	AT5G60570	71.468	361	102	1	5	364	33	393	0.0	537
MS.gene42821.t1	AT5G60570	71.468	361	102	1	5	364	33	393	0.0	537
MS.gene42821.t1	AT5G60570	71.468	361	102	1	5	364	33	393	0.0	537
MS.gene42821.t1	AT1G26930	47.861	374	177	5	2	364	55	421	1.01e-117	348
MS.gene42821.t1	AT1G14330	47.967	369	180	6	2	364	79	441	7.32e-113	336
MS.gene42821.t1	AT2G02870	47.326	374	183	5	1	364	98	467	2.44e-112	335
MS.gene42821.t1	AT2G02870	47.326	374	183	5	1	364	98	467	2.44e-112	335
MS.gene42821.t1	AT2G02870	47.326	374	183	5	1	364	98	467	2.44e-112	335
MS.gene42821.t1	AT1G74510	46.499	357	171	4	24	364	99	451	1.02e-105	318
MS.gene42821.t1	AT1G74510	46.499	357	171	4	24	364	99	451	1.02e-105	318
MS.gene42821.t1	AT1G74510	46.499	357	171	4	24	364	99	451	1.02e-105	318
MS.gene42821.t1	AT1G26930	39.305	374	157	7	2	364	55	369	6.12e-82	254
MS.gene42821.t1	AT3G27150	39.660	353	199	7	21	362	71	420	2.53e-80	252
MS.gene42821.t1	AT3G27150	39.660	353	199	7	21	362	71	420	2.53e-80	252
MS.gene42821.t1	AT5G40680	35.475	358	205	7	21	362	66	413	4.82e-68	220
MS.gene42821.t1	AT1G55270	29.932	294	180	11	8	287	66	347	1.15e-25	107
MS.gene42821.t1	AT3G63220	26.524	328	198	11	13	321	4	307	2.41e-25	105
MS.gene42821.t1	AT3G63220	26.524	328	198	11	13	321	4	307	2.41e-25	105
MS.gene42821.t1	AT3G63220	26.791	321	192	11	20	321	4	300	4.92e-25	104
MS.gene42821.t1	AT1G16250	23.529	323	223	7	17	317	5	325	3.49e-23	99.8
MS.gene42821.t1	AT1G16250	23.529	323	223	7	17	317	5	325	3.49e-23	99.8
MS.gene42821.t1	AT1G67480	25.478	314	211	8	17	318	36	338	7.66e-22	95.9
MS.gene42821.t1	AT1G67480	25.478	314	211	8	17	318	36	338	7.66e-22	95.9
MS.gene42821.t1	AT1G67480	25.478	314	211	8	17	318	36	338	7.66e-22	95.9
MS.gene42821.t1	AT1G15670	27.746	346	198	15	20	330	3	331	1.34e-16	80.5
MS.gene42821.t1	AT3G61350	24.779	339	217	13	19	334	20	343	3.95e-16	79.3
MS.gene42821.t1	AT3G61350	24.779	339	217	13	19	334	20	343	3.95e-16	79.3
MS.gene42821.t1	AT1G80440	24.771	327	199	11	20	316	3	312	2.64e-15	76.6
MS.gene42821.t1	AT1G22040	24.513	359	192	15	20	303	43	397	4.16e-15	76.6
MS.gene42821.t1	AT5G02980	27.928	222	113	7	16	237	9	183	2.65e-14	73.6
MS.gene42821.t1	AT1G30090	24.912	285	195	7	18	288	51	330	5.73e-14	73.2
MS.gene42821.t1	AT3G59940	26.936	297	162	10	20	261	15	311	1.87e-13	71.6
MS.gene42821.t1	AT2G21950	23.200	375	248	13	9	359	9	367	5.09e-13	70.1
MS.gene42821.t1	AT5G03000	28.448	232	141	8	19	231	38	263	5.43e-12	66.6
MS.gene42821.t1	AT1G27420	21.646	328	201	11	20	313	10	315	2.38e-11	64.7
MS.gene42821.t1	AT4G19870	27.570	214	127	5	26	238	35	221	6.42e-11	63.9
MS.gene42821.t1	AT4G19870	27.570	214	127	5	26	238	35	221	6.42e-11	63.9
MS.gene42821.t1	AT1G19460	22.840	324	205	9	17	317	51	352	8.41e-11	63.5

Find 111 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTGGAACATTTGGTTTATT+TGG	0.199868	2.3:-7810926	MS.gene42821:CDS
CACTGTCTTGTATTAATAAA+AGG	0.245075	2.3:-7811005	MS.gene42821:CDS
CCATCCCTGACACCGGATTT+AGG	0.245877	2.3:+7810139	None:intergenic
ATCAGTAGTGGTTATTTATA+TGG	0.252399	2.3:-7810970	MS.gene42821:CDS
TGGCTGGGGCCTTGCTTTCA+AGG	0.285708	2.3:-7810239	MS.gene42821:CDS
AACCAACCTCGCTGTTTGTT+TGG	0.309371	2.3:-7810691	MS.gene42821:CDS
AAAGAACACTTGGAATGAAT+TGG	0.319362	2.3:-7810293	MS.gene42821:CDS
GGGCTGATTCTTCAAATGGC+TGG	0.323493	2.3:-7810255	MS.gene42821:CDS
GGCTGATTCTTCAAATGGCT+GGG	0.327405	2.3:-7810254	MS.gene42821:CDS
GGCTGTGGGTTGTGAACTCT+TGG	0.337719	2.3:-7810794	MS.gene42821:CDS
ATATGGATTGCGGAAAGAAT+TGG	0.349734	2.3:-7810953	MS.gene42821:CDS
AGCACGATAGCTTCACCTTC+TGG	0.354930	2.3:+7810175	None:intergenic
GCCGGTGGAAGTGATAAATA+TGG	0.355398	2.3:-7810637	MS.gene42821:CDS
ATTGGGGAGGCTCCCAGTTC+GGG	0.361540	2.3:-7810275	MS.gene42821:CDS
ATTGGGAGTTGTGGAACATT+TGG	0.362570	2.3:-7810935	MS.gene42821:CDS
CACTCATCGCACGGTATCTT+AGG	0.371909	2.3:+7810844	None:intergenic
TTGCCCTCAATTGTCTCGCT+TGG	0.372370	2.3:-7811050	MS.gene42821:CDS
AAGAACACTTGGAATGAATT+GGG	0.376731	2.3:-7810292	MS.gene42821:CDS
TCGGTTTGAAGGAGCATGTC+GGG	0.388214	2.3:-7810096	MS.gene42821:CDS
GGCTTGTGGAGATAAACTTT+TGG	0.389229	2.3:-7810218	MS.gene42821:CDS
TATGGATTGCGGAAAGAATT+GGG	0.390725	2.3:-7810952	MS.gene42821:CDS
GGTGTCCTAAATCCGGTGTC+AGG	0.395086	2.3:-7810144	MS.gene42821:CDS
AAGAAGTTGGAGGAAAATAG+AGG	0.402405	2.3:-7810431	MS.gene42821:CDS
GATGGTAAATTCTATGTTAT+CGG	0.408896	2.3:-7810511	MS.gene42821:CDS
TGCCCTCAATTGTCTCGCTT+GGG	0.408983	2.3:-7811049	MS.gene42821:CDS
TTTGAAGAATCAGCCCGAAC+TGG	0.411166	2.3:+7810262	None:intergenic
GTATTAATAAAAGGTATAGT+AGG	0.411880	2.3:-7810996	MS.gene42821:CDS
CTACGCGGTGTGTGCATGTT+TGG	0.416639	2.3:+7810556	None:intergenic
GTTCGGGCTGATTCTTCAAA+TGG	0.420110	2.3:-7810259	MS.gene42821:CDS
AATTGGGGAGGCTCCCAGTT+CGG	0.420940	2.3:-7810276	MS.gene42821:CDS
TTTGGTTGTGGGTGGACAAA+GGG	0.421209	2.3:-7810200	MS.gene42821:CDS
GGCCTTGCTTTCAAGGCTTG+TGG	0.422359	2.3:-7810232	MS.gene42821:CDS
TTGTTTGGATCTAGTAGTCT+TGG	0.429145	2.3:-7810676	MS.gene42821:CDS
AATTGATGGAGTTCGCCATT+TGG	0.441296	2.3:-7810759	MS.gene42821:CDS
ATGATCTTTCAACAAGAAGT+TGG	0.446580	2.3:-7810444	MS.gene42821:CDS
CAGCGAGGTTGGTTCATCTC+TGG	0.457367	2.3:+7810700	None:intergenic
AAGCTATCGTGCTGAATTCA+TGG	0.466217	2.3:-7810165	MS.gene42821:CDS
TTGGCAGGTACTCGGTTTGA+AGG	0.470109	2.3:-7810107	MS.gene42821:CDS
TCAGGGATGGCATCATTGAT+TGG	0.476602	2.3:-7810126	MS.gene42821:CDS
TGCTCAAGCACCTCCACTTG+TGG	0.477930	2.3:-7810383	MS.gene42821:CDS
CTCAATTGTCTCGCTTGGGT+TGG	0.478795	2.3:-7811045	MS.gene42821:CDS
TCCATATTTATCACTTCCAC+CGG	0.480339	2.3:+7810636	None:intergenic
GGTTCAATTGCTATTGTAGC+CGG	0.481224	2.3:-7810655	MS.gene42821:CDS
CCAGGGAGAAGCGAATCATT+TGG	0.482859	2.3:+7811087	None:intergenic
ATCATTGATTGGCAGGTACT+CGG	0.486116	2.3:-7810115	MS.gene42821:CDS
TCAAACGCCACCCATCCTCT+TGG	0.488532	2.3:+7810895	None:intergenic
GGATGGCATCATTGATTGGC+AGG	0.490041	2.3:-7810122	MS.gene42821:CDS
GGACAAAGAGTCCTTGGCTG+TGG	0.495417	2.3:-7810809	MS.gene42821:CDS
TGAGCACCCACATTAACATA+TGG	0.504068	2.3:+7810400	None:intergenic
TTGACCCTACCATAGGTAGA+TGG	0.507495	2.3:-7810876	MS.gene42821:CDS
CAACCCAAGCGAGACAATTG+AGG	0.507498	2.3:+7811046	None:intergenic
ACAAGACAGTGACGCGTGAT+CGG	0.508795	2.3:+7811017	None:intergenic
CCACGCGGACAAAGAGTCCT+TGG	0.511167	2.3:-7810815	MS.gene42821:CDS
AATTCATGGTGTCCTAAATC+CGG	0.511637	2.3:-7810151	MS.gene42821:CDS
CTCCACAAGCCTTGAAAGCA+AGG	0.517282	2.3:+7810230	None:intergenic
CAGAGGGAAGGAGTGAATGA+TGG	0.519298	2.3:-7811126	MS.gene42821:CDS
GAATCATTTGGTCTATGTCT+AGG	0.523572	2.3:+7811099	None:intergenic
ACCTCCACTTGTGGCAGTTG+TGG	0.526349	2.3:-7810374	MS.gene42821:CDS
CTTAAAATGGATCAGAGGGA+AGG	0.531777	2.3:-7811138	None:intergenic
CAACGCCATCCATCTACCTA+TGG	0.534689	2.3:+7810867	None:intergenic
ATGACTCTGCATCATGTACA+TGG	0.536787	2.3:-7810588	MS.gene42821:CDS
AATCAAGCTATACTTCCAAA+TGG	0.541475	2.3:+7810744	None:intergenic
AACCCAAGCGAGACAATTGA+GGG	0.543072	2.3:+7811047	None:intergenic
CTCGGTTTGAAGGAGCATGT+CGG	0.543892	2.3:-7810097	MS.gene42821:CDS
TTGAAGAATCAGCCCGAACT+GGG	0.544400	2.3:+7810263	None:intergenic
GCGGAAAGAATTGGGAGTTG+TGG	0.547490	2.3:-7810944	MS.gene42821:CDS
ATAGCTTGATTTGCCGTCGT+TGG	0.547592	2.3:-7810732	MS.gene42821:CDS
GCAACATCATCGATCAGACC+AGG	0.551069	2.3:+7811069	None:intergenic
ATCCAAACAAACAGCGAGGT+TGG	0.552845	2.3:+7810689	None:intergenic
ATCTTTCAACAAGAAGTTGG+AGG	0.559527	2.3:-7810441	MS.gene42821:CDS
ACAACTGTTTCATTAACTTG+TGG	0.560696	2.3:-7810475	MS.gene42821:CDS
CCTAAATCCGGTGTCAGGGA+TGG	0.560965	2.3:-7810139	MS.gene42821:CDS
CCAAATGATTCGCTTCTCCC+TGG	0.562574	2.3:-7811087	MS.gene42821:CDS
GTGGTTATTTATATGGATTG+CGG	0.565721	2.3:-7810963	MS.gene42821:CDS
TATAGTAGGTTGATCAGTAG+TGG	0.567034	2.3:-7810982	MS.gene42821:CDS
GCTGATTCTTCAAATGGCTG+GGG	0.569460	2.3:-7810253	MS.gene42821:CDS
TTGGTTGTGGGTGGACAAAG+GGG	0.570955	2.3:-7810199	MS.gene42821:CDS
TGGTTTGTGATCCAAGAGGA+TGG	0.572503	2.3:-7810906	MS.gene42821:CDS
AGAAGTTGGAGGAAAATAGA+GGG	0.574262	2.3:-7810430	MS.gene42821:CDS
GGTGGACAAAGGGGTCCAGA+AGG	0.576618	2.3:-7810190	MS.gene42821:CDS
GACAAAGAGTCCTTGGCTGT+GGG	0.576875	2.3:-7810808	MS.gene42821:CDS
GTGCTGTTATGAGCTGTTGA+GGG	0.579091	2.3:-7810057	None:intergenic
TATGTATCCATATGTTAATG+TGG	0.581465	2.3:-7810407	MS.gene42821:CDS
TCAATTGCTATTGTAGCCGG+TGG	0.583147	2.3:-7810652	MS.gene42821:CDS
GGTAAATTCTATGTTATCGG+TGG	0.585329	2.3:-7810508	MS.gene42821:CDS
GTGTCCTAAATCCGGTGTCA+GGG	0.587917	2.3:-7810143	MS.gene42821:CDS
ATAACAAGCAAAAGAACACT+TGG	0.593822	2.3:-7810303	MS.gene42821:CDS
TCCACAACTGCCACAAGTGG+AGG	0.600198	2.3:+7810373	None:intergenic
AATGATGCCATCCCTGACAC+CGG	0.601414	2.3:+7810132	None:intergenic
GGTTTGTGATCCAAGAGGAT+GGG	0.603205	2.3:-7810905	MS.gene42821:CDS
CCATCCATCTACCTATGGTA+GGG	0.606052	2.3:+7810872	None:intergenic
TGTGCTGTTATGAGCTGTTG+AGG	0.607927	2.3:-7810058	None:intergenic
CCCTACCATAGGTAGATGGA+TGG	0.610147	2.3:-7810872	MS.gene42821:CDS
GCCATCCATCTACCTATGGT+AGG	0.612392	2.3:+7810871	None:intergenic
GAGTTCACAACCCACAGCCA+AGG	0.616056	2.3:+7810798	None:intergenic
TTATCCACAACTGCCACAAG+TGG	0.623897	2.3:+7810370	None:intergenic
AGAACACTTGGAATGAATTG+GGG	0.627130	2.3:-7810291	MS.gene42821:CDS
GTGGCGTTTGACCCTACCAT+AGG	0.627159	2.3:-7810883	MS.gene42821:CDS
ATGTATCCATATGTTAATGT+GGG	0.634893	2.3:-7810406	MS.gene42821:CDS
CGGTTTGAAGGAGCATGTCG+GGG	0.636704	2.3:-7810095	MS.gene42821:CDS
CCAAGGACTCTTTGTCCGCG+TGG	0.654382	2.3:+7810815	None:intergenic
TGGTTAAAACACTCATCGCA+CGG	0.658387	2.3:+7810835	None:intergenic
AGTTGAGCACTTAACTAACA+TGG	0.659156	2.3:-7810335	MS.gene42821:CDS
ACACTTGGAATGAATTGGGG+AGG	0.662795	2.3:-7810288	MS.gene42821:CDS
TTGTGATCCAAGAGGATGGG+TGG	0.673255	2.3:-7810902	MS.gene42821:CDS
CAACATCATCGATCAGACCA+GGG	0.673678	2.3:+7811070	None:intergenic
CTGGACACTTCAACCAACGA+CGG	0.676455	2.3:+7810719	None:intergenic
TATTTGGTTTGTGATCCAAG+AGG	0.692041	2.3:-7810910	MS.gene42821:CDS
TGACTCTGCATCATGTACAT+GGG	0.735594	2.3:-7810587	MS.gene42821:CDS
AAACTAGAGCACAATCTACG+CGG	0.755040	2.3:+7810541	None:intergenic
CTAGATCCAAACAAACAGCG+AGG	0.779810	2.3:+7810685	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	GTATTAATAAAAGGTATAGT+AGG	-	chr2.3:7810196-7810215	MS.gene42821:CDS	20.0%
!	ATCAGTAGTGGTTATTTATA+TGG	-	chr2.3:7810222-7810241	MS.gene42821:CDS	25.0%
!	ATGTATCCATATGTTAATGT+GGG	-	chr2.3:7810786-7810805	MS.gene42821:CDS	25.0%
!	CACTGTCTTGTATTAATAAA+AGG	-	chr2.3:7810187-7810206	MS.gene42821:CDS	25.0%
!	GATGGTAAATTCTATGTTAT+CGG	-	chr2.3:7810681-7810700	MS.gene42821:CDS	25.0%
!	TATGTATCCATATGTTAATG+TGG	-	chr2.3:7810785-7810804	MS.gene42821:CDS	25.0%
!!!	ATTGTGCTCTAGTTTTTTTA+TGG	-	chr2.3:7810659-7810678	MS.gene42821:CDS	25.0%
	AAAGAACACTTGGAATGAAT+TGG	-	chr2.3:7810899-7810918	MS.gene42821:CDS	30.0%
	AAGAACACTTGGAATGAATT+GGG	-	chr2.3:7810900-7810919	MS.gene42821:CDS	30.0%
	AATCAAGCTATACTTCCAAA+TGG	+	chr2.3:7810451-7810470	None:intergenic	30.0%
	ATAACAAGCAAAAGAACACT+TGG	-	chr2.3:7810889-7810908	MS.gene42821:CDS	30.0%
	ATGATCTTTCAACAAGAAGT+TGG	-	chr2.3:7810748-7810767	MS.gene42821:CDS	30.0%
!	ACAACTGTTTCATTAACTTG+TGG	-	chr2.3:7810717-7810736	MS.gene42821:CDS	30.0%
!	GTGGTTATTTATATGGATTG+CGG	-	chr2.3:7810229-7810248	MS.gene42821:CDS	30.0%
!	TGTGGAACATTTGGTTTATT+TGG	-	chr2.3:7810266-7810285	MS.gene42821:CDS	30.0%
!!!	TGCTCTAGTTTTTTTATGGA+TGG	-	chr2.3:7810663-7810682	MS.gene42821:CDS	30.0%
	AAGAAGTTGGAGGAAAATAG+AGG	-	chr2.3:7810761-7810780	MS.gene42821:CDS	35.0%
	AATTCATGGTGTCCTAAATC+CGG	-	chr2.3:7811041-7811060	MS.gene42821:CDS	35.0%
	AGAACACTTGGAATGAATTG+GGG	-	chr2.3:7810901-7810920	MS.gene42821:CDS	35.0%
	AGAAGTTGGAGGAAAATAGA+GGG	-	chr2.3:7810762-7810781	MS.gene42821:CDS	35.0%
	AGTTGAGCACTTAACTAACA+TGG	-	chr2.3:7810857-7810876	MS.gene42821:CDS	35.0%
	ATCTTTCAACAAGAAGTTGG+AGG	-	chr2.3:7810751-7810770	MS.gene42821:CDS	35.0%
	GAATCATTTGGTCTATGTCT+AGG	+	chr2.3:7810096-7810115	None:intergenic	35.0%
	GGTAAATTCTATGTTATCGG+TGG	-	chr2.3:7810684-7810703	MS.gene42821:CDS	35.0%
	TCCATATTTATCACTTCCAC+CGG	+	chr2.3:7810559-7810578	None:intergenic	35.0%
	TTGTTTGGATCTAGTAGTCT+TGG	-	chr2.3:7810516-7810535	MS.gene42821:CDS	35.0%
!	ATATGGATTGCGGAAAGAAT+TGG	-	chr2.3:7810239-7810258	MS.gene42821:CDS	35.0%
!	TATGGATTGCGGAAAGAATT+GGG	-	chr2.3:7810240-7810259	MS.gene42821:CDS	35.0%
!!	GGAGATAAACTTTTGGTTGT+GGG	-	chr2.3:7810981-7811000	MS.gene42821:CDS	35.0%
!!	TATAGTAGGTTGATCAGTAG+TGG	-	chr2.3:7810210-7810229	MS.gene42821:CDS	35.0%
!!	TATTTGGTTTGTGATCCAAG+AGG	-	chr2.3:7810282-7810301	MS.gene42821:CDS	35.0%
!!	TGGAGATAAACTTTTGGTTG+TGG	-	chr2.3:7810980-7810999	MS.gene42821:CDS	35.0%
!!!	TGAACTCTTGGTTTTTGGTA+GGG	-	chr2.3:7810410-7810429	MS.gene42821:CDS	35.0%
	AAACTAGAGCACAATCTACG+CGG	+	chr2.3:7810654-7810673	None:intergenic	40.0%
	AAGCTATCGTGCTGAATTCA+TGG	-	chr2.3:7811027-7811046	MS.gene42821:CDS	40.0%
	AATTGATGGAGTTCGCCATT+TGG	-	chr2.3:7810433-7810452	MS.gene42821:CDS	40.0%
	ATGACTCTGCATCATGTACA+TGG	-	chr2.3:7810604-7810623	MS.gene42821:CDS	40.0%
	ATTGGGAGTTGTGGAACATT+TGG	-	chr2.3:7810257-7810276	MS.gene42821:CDS	40.0%
	TGACTCTGCATCATGTACAT+GGG	-	chr2.3:7810605-7810624	MS.gene42821:CDS	40.0%
	TGAGCACCCACATTAACATA+TGG	+	chr2.3:7810795-7810814	None:intergenic	40.0%
	TGGTTAAAACACTCATCGCA+CGG	+	chr2.3:7810360-7810379	None:intergenic	40.0%
!	ATCATTGATTGGCAGGTACT+CGG	-	chr2.3:7811077-7811096	MS.gene42821:CDS	40.0%
!	GGCTTGTGGAGATAAACTTT+TGG	-	chr2.3:7810974-7810993	MS.gene42821:CDS	40.0%
!	GGTTCAATTGCTATTGTAGC+CGG	-	chr2.3:7810537-7810556	MS.gene42821:CDS	40.0%
!!	GATAAACTTTTGGTTGTGGG+TGG	-	chr2.3:7810984-7811003	MS.gene42821:CDS	40.0%
!!	GGTTGTGAACTCTTGGTTTT+TGG	-	chr2.3:7810405-7810424	MS.gene42821:CDS	40.0%
!!!	GGTTTTTGGTAGGGAATTGA+TGG	-	chr2.3:7810419-7810438	MS.gene42821:CDS	40.0%
!!!	GTGAACTCTTGGTTTTTGGT+AGG	-	chr2.3:7810409-7810428	MS.gene42821:CDS	40.0%
	AACCAACCTCGCTGTTTGTT+TGG	-	chr2.3:7810501-7810520	MS.gene42821:CDS	45.0%
	AACCCAAGCGAGACAATTGA+GGG	+	chr2.3:7810148-7810167	None:intergenic	45.0%
	ACACTTGGAATGAATTGGGG+AGG	-	chr2.3:7810904-7810923	MS.gene42821:CDS	45.0%
	ATCCAAACAAACAGCGAGGT+TGG	+	chr2.3:7810506-7810525	None:intergenic	45.0%
	CAACATCATCGATCAGACCA+GGG	+	chr2.3:7810125-7810144	None:intergenic	45.0%
	CCATCCATCTACCTATGGTA+GGG	+	chr2.3:7810323-7810342	None:intergenic	45.0%
	CTAGATCCAAACAAACAGCG+AGG	+	chr2.3:7810510-7810529	None:intergenic	45.0%
	GCCGGTGGAAGTGATAAATA+TGG	-	chr2.3:7810555-7810574	MS.gene42821:CDS	45.0%
	GGTTTGTGATCCAAGAGGAT+GGG	-	chr2.3:7810287-7810306	MS.gene42821:CDS	45.0%
	TCAGGGATGGCATCATTGAT+TGG	-	chr2.3:7811066-7811085	MS.gene42821:CDS	45.0%
	TTATCCACAACTGCCACAAG+TGG	+	chr2.3:7810825-7810844	None:intergenic	45.0%
	TTGAAGAATCAGCCCGAACT+GGG	+	chr2.3:7810932-7810951	None:intergenic	45.0%
	TTGACCCTACCATAGGTAGA+TGG	-	chr2.3:7810316-7810335	MS.gene42821:CDS	45.0%
	TTTGAAGAATCAGCCCGAAC+TGG	+	chr2.3:7810933-7810952	None:intergenic	45.0%
	TTTGGTTGTGGGTGGACAAA+GGG	-	chr2.3:7810992-7811011	MS.gene42821:CDS	45.0%
!	GCTGATTCTTCAAATGGCTG+GGG	-	chr2.3:7810939-7810958	MS.gene42821:CDS	45.0%
!	GGCTGATTCTTCAAATGGCT+GGG	-	chr2.3:7810938-7810957	MS.gene42821:CDS	45.0%
!	GTTCGGGCTGATTCTTCAAA+TGG	-	chr2.3:7810933-7810952	MS.gene42821:CDS	45.0%
!	TCAATTGCTATTGTAGCCGG+TGG	-	chr2.3:7810540-7810559	MS.gene42821:CDS	45.0%
!	TGGTTTGTGATCCAAGAGGA+TGG	-	chr2.3:7810286-7810305	MS.gene42821:CDS	45.0%
!!	ATAGCTTGATTTGCCGTCGT+TGG	-	chr2.3:7810460-7810479	MS.gene42821:CDS	45.0%
!!	TTTTGGTTGTGGGTGGACAA+AGG	-	chr2.3:7810991-7811010	MS.gene42821:CDS	45.0%
!!!	CGATGAGTGTTTTAACCACG+CGG	-	chr2.3:7810362-7810381	MS.gene42821:CDS	45.0%
	AATGATGCCATCCCTGACAC+CGG	+	chr2.3:7811063-7811082	None:intergenic	50.0%
	ACAAGACAGTGACGCGTGAT+CGG	+	chr2.3:7810178-7810197	None:intergenic	50.0%
	AGCACGATAGCTTCACCTTC+TGG	+	chr2.3:7811020-7811039	None:intergenic	50.0%
	CAACCCAAGCGAGACAATTG+AGG	+	chr2.3:7810149-7810168	None:intergenic	50.0%
	CAACGCCATCCATCTACCTA+TGG	+	chr2.3:7810328-7810347	None:intergenic	50.0%
	CACTCATCGCACGGTATCTT+AGG	+	chr2.3:7810351-7810370	None:intergenic	50.0%
	CAGAGGGAAGGAGTGAATGA+TGG	-	chr2.3:7810066-7810085	MS.gene42821:CDS	50.0%
	CCAAATGATTCGCTTCTCCC+TGG	-	chr2.3:7810105-7810124	MS.gene42821:CDS	50.0%
	CCAGGGAGAAGCGAATCATT+TGG	+	chr2.3:7810108-7810127	None:intergenic	50.0%
	CCCTACCATAGGTAGATGGA+TGG	-	chr2.3:7810320-7810339	MS.gene42821:CDS	50.0%
	CTCAATTGTCTCGCTTGGGT+TGG	-	chr2.3:7810147-7810166	MS.gene42821:CDS	50.0%
	CTCCACAAGCCTTGAAAGCA+AGG	+	chr2.3:7810965-7810984	None:intergenic	50.0%
	CTGGACACTTCAACCAACGA+CGG	+	chr2.3:7810476-7810495	None:intergenic	50.0%
	GACAAAGAGTCCTTGGCTGT+GGG	-	chr2.3:7810384-7810403	MS.gene42821:CDS	50.0%
	GCAACATCATCGATCAGACC+AGG	+	chr2.3:7810126-7810145	None:intergenic	50.0%
	GCCATCCATCTACCTATGGT+AGG	+	chr2.3:7810324-7810343	None:intergenic	50.0%
	GCGGAAAGAATTGGGAGTTG+TGG	-	chr2.3:7810248-7810267	MS.gene42821:CDS	50.0%
	TGCCCTCAATTGTCTCGCTT+GGG	-	chr2.3:7810143-7810162	MS.gene42821:CDS	50.0%
	TTGCCCTCAATTGTCTCGCT+TGG	-	chr2.3:7810142-7810161	MS.gene42821:CDS	50.0%
	TTGGTTGTGGGTGGACAAAG+GGG	-	chr2.3:7810993-7811012	MS.gene42821:CDS	50.0%
	TTGTGATCCAAGAGGATGGG+TGG	-	chr2.3:7810290-7810309	MS.gene42821:CDS	50.0%
!	CTCGGTTTGAAGGAGCATGT+CGG	-	chr2.3:7811095-7811114	MS.gene42821:CDS	50.0%
!	GGATGGCATCATTGATTGGC+AGG	-	chr2.3:7811070-7811089	MS.gene42821:CDS	50.0%
!	GGGCTGATTCTTCAAATGGC+TGG	-	chr2.3:7810937-7810956	MS.gene42821:CDS	50.0%
!	GTGTCCTAAATCCGGTGTCA+GGG	-	chr2.3:7811049-7811068	MS.gene42821:CDS	50.0%
!	TCGGTTTGAAGGAGCATGTC+GGG	-	chr2.3:7811096-7811115	MS.gene42821:CDS	50.0%
!	TTGGCAGGTACTCGGTTTGA+AGG	-	chr2.3:7811085-7811104	MS.gene42821:CDS	50.0%
	AATTGGGGAGGCTCCCAGTT+CGG	-	chr2.3:7810916-7810935	MS.gene42821:CDS	55.0%
	ACCTCCACTTGTGGCAGTTG+TGG	-	chr2.3:7810818-7810837	MS.gene42821:CDS	55.0%
	CCATCCCTGACACCGGATTT+AGG	+	chr2.3:7811056-7811075	None:intergenic	55.0%
	CTACGCGGTGTGTGCATGTT+TGG	+	chr2.3:7810639-7810658	None:intergenic	55.0%
	GAGTTCACAACCCACAGCCA+AGG	+	chr2.3:7810397-7810416	None:intergenic	55.0%
	GGACAAAGAGTCCTTGGCTG+TGG	-	chr2.3:7810383-7810402	MS.gene42821:CDS	55.0%
	GGCCTTGCTTTCAAGGCTTG+TGG	-	chr2.3:7810960-7810979	MS.gene42821:CDS	55.0%
	GGTGTCCTAAATCCGGTGTC+AGG	-	chr2.3:7811048-7811067	MS.gene42821:CDS	55.0%
	GTGGCGTTTGACCCTACCAT+AGG	-	chr2.3:7810309-7810328	MS.gene42821:CDS	55.0%
	TCAAACGCCACCCATCCTCT+TGG	+	chr2.3:7810300-7810319	None:intergenic	55.0%
	TCCACAACTGCCACAAGTGG+AGG	+	chr2.3:7810822-7810841	None:intergenic	55.0%
	TGCTCAAGCACCTCCACTTG+TGG	-	chr2.3:7810809-7810828	MS.gene42821:CDS	55.0%
!	CAGCGAGGTTGGTTCATCTC+TGG	+	chr2.3:7810495-7810514	None:intergenic	55.0%
!	CCTAAATCCGGTGTCAGGGA+TGG	-	chr2.3:7811053-7811072	MS.gene42821:CDS	55.0%
!	CGGTTTGAAGGAGCATGTCG+GGG	-	chr2.3:7811097-7811116	MS.gene42821:CDS	55.0%
!	GGCTGTGGGTTGTGAACTCT+TGG	-	chr2.3:7810398-7810417	MS.gene42821:CDS	55.0%
	ATTGGGGAGGCTCCCAGTTC+GGG	-	chr2.3:7810917-7810936	MS.gene42821:CDS	60.0%
	CCAAGGACTCTTTGTCCGCG+TGG	+	chr2.3:7810380-7810399	None:intergenic	60.0%
	CCACGCGGACAAAGAGTCCT+TGG	-	chr2.3:7810377-7810396	MS.gene42821:CDS	60.0%
	GGTGGACAAAGGGGTCCAGA+AGG	-	chr2.3:7811002-7811021	MS.gene42821:CDS	60.0%
	TGGCTGGGGCCTTGCTTTCA+AGG	-	chr2.3:7810953-7810972	MS.gene42821:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	7810060	7811154	7810060	ID=MS.gene42821
chr2.3	mRNA	7810060	7811154	7810060	ID=MS.gene42821.t1;Parent=MS.gene42821
chr2.3	exon	7810060	7811154	7810060	ID=MS.gene42821.t1.exon1;Parent=MS.gene42821.t1
chr2.3	CDS	7810060	7811154	7810060	ID=cds.MS.gene42821.t1;Parent=MS.gene42821.t1

Gene Sequence

>MS.gene42821

ATGGATCAGAGGGAAGGAGTGAATGATGGTTTTCCTAGACATAGACCAAATGATTCGCTTCTCCCTGGTCTGATCGATGATGTTGCCCTCAATTGTCTCGCTTGGGTTGGTAGATCCGATCACGCGTCACTGTCTTGTATTAATAAAAGGTATAGTAGGTTGATCAGTAGTGGTTATTTATATGGATTGCGGAAAGAATTGGGAGTTGTGGAACATTTGGTTTATTTGGTTTGTGATCCAAGAGGATGGGTGGCGTTTGACCCTACCATAGGTAGATGGATGGCGTTGCCTAAGATACCGTGCGATGAGTGTTTTAACCACGCGGACAAAGAGTCCTTGGCTGTGGGTTGTGAACTCTTGGTTTTTGGTAGGGAATTGATGGAGTTCGCCATTTGGAAGTATAGCTTGATTTGCCGTCGTTGGTTGAAGTGTCCAGAGATGAACCAACCTCGCTGTTTGTTTGGATCTAGTAGTCTTGGTTCAATTGCTATTGTAGCCGGTGGAAGTGATAAATATGGAAATGTTCTGAAGTCTGCTGAGTTGTATGACTCTGCATCATGTACATGGGAACTTTTACCAAACATGCACACACCGCGTAGATTGTGCTCTAGTTTTTTTATGGATGGTAAATTCTATGTTATCGGTGGAATGTCGAGCACAACTGTTTCATTAACTTGTGGAGAAGAATATGATCTTTCAACAAGAAGTTGGAGGAAAATAGAGGGTATGTATCCATATGTTAATGTGGGTGCTCAAGCACCTCCACTTGTGGCAGTTGTGGATAATCAGTTGTATGCAGTTGAGCACTTAACTAACATGGTGAAGATGTATAACAAGCAAAAGAACACTTGGAATGAATTGGGGAGGCTCCCAGTTCGGGCTGATTCTTCAAATGGCTGGGGCCTTGCTTTCAAGGCTTGTGGAGATAAACTTTTGGTTGTGGGTGGACAAAGGGGTCCAGAAGGTGAAGCTATCGTGCTGAATTCATGGTGTCCTAAATCCGGTGTCAGGGATGGCATCATTGATTGGCAGGTACTCGGTTTGAAGGAGCATGTCGGGGTGTTCGTGTATAATTGTGCTGTTATGAGCTGTTGA

Protein sequence

>MS.gene42821.t1

MDQREGVNDGFPRHRPNDSLLPGLIDDVALNCLAWVGRSDHASLSCINKRYSRLISSGYLYGLRKELGVVEHLVYLVCDPRGWVAFDPTIGRWMALPKIPCDECFNHADKESLAVGCELLVFGRELMEFAIWKYSLICRRWLKCPEMNQPRCLFGSSSLGSIAIVAGGSDKYGNVLKSAELYDSASCTWELLPNMHTPRRLCSSFFMDGKFYVIGGMSSTTVSLTCGEEYDLSTRSWRKIEGMYPYVNVGAQAPPLVAVVDNQLYAVEHLTNMVKMYNKQKNTWNELGRLPVRADSSNGWGLAFKACGDKLLVVGGQRGPEGEAIVLNSWCPKSGVRDGIIDWQVLGLKEHVGVFVYNCAVMSC