Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56132.t1 | XP_003621734.2 | 92.6 | 367 | 27 | 0 | 1 | 367 | 1 | 367 | 2.40E-165 | 591.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56132.t1 | G7KUQ5 | 92.6 | 367 | 27 | 0 | 1 | 367 | 1 | 367 | 1.7e-165 | 591.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050114 | MS.gene56132 | 0.822838 | 1.91E-53 | -1.69E-46 |
| MS.gene050277 | MS.gene56132 | 0.805954 | 1.04E-49 | -1.69E-46 |
| MS.gene050317 | MS.gene56132 | 0.83413 | 3.58E-56 | -1.69E-46 |
| MS.gene050320 | MS.gene56132 | 0.800282 | 1.55E-48 | -1.69E-46 |
| MS.gene051245 | MS.gene56132 | 0.828472 | 8.82E-55 | -1.69E-46 |
| MS.gene051746 | MS.gene56132 | 0.802672 | 5.02E-49 | -1.69E-46 |
| MS.gene051748 | MS.gene56132 | 0.828432 | 9.02E-55 | -1.69E-46 |
| MS.gene051756 | MS.gene56132 | 0.835715 | 1.43E-56 | -1.69E-46 |
| MS.gene051903 | MS.gene56132 | 0.835351 | 1.77E-56 | -1.69E-46 |
| MS.gene052434 | MS.gene56132 | 0.808289 | 3.33E-50 | -1.69E-46 |
| MS.gene053109 | MS.gene56132 | 0.811336 | 7.37E-51 | -1.69E-46 |
| MS.gene05344 | MS.gene56132 | -0.811473 | 6.88E-51 | -1.69E-46 |
| MS.gene055441 | MS.gene56132 | 0.80575 | 1.15E-49 | -1.69E-46 |
| MS.gene056787 | MS.gene56132 | 0.83237 | 9.83E-56 | -1.69E-46 |
| MS.gene056986 | MS.gene56132 | 0.809206 | 2.12E-50 | -1.69E-46 |
| MS.gene057079 | MS.gene56132 | 0.807979 | 3.88E-50 | -1.69E-46 |
| MS.gene057094 | MS.gene56132 | 0.805589 | 1.24E-49 | -1.69E-46 |
| MS.gene057140 | MS.gene56132 | 0.828516 | 8.61E-55 | -1.69E-46 |
| MS.gene057234 | MS.gene56132 | 0.845889 | 3.06E-59 | -1.69E-46 |
| MS.gene057999 | MS.gene56132 | 0.829904 | 3.96E-55 | -1.69E-46 |
| MS.gene058055 | MS.gene56132 | 0.804699 | 1.91E-49 | -1.69E-46 |
| MS.gene058182 | MS.gene56132 | 0.815414 | 9.36E-52 | -1.69E-46 |
| MS.gene058238 | MS.gene56132 | 0.842261 | 2.88E-58 | -1.69E-46 |
| MS.gene058654 | MS.gene56132 | 0.818345 | 2.06E-52 | -1.69E-46 |
| MS.gene058906 | MS.gene56132 | 0.801695 | 7.97E-49 | -1.69E-46 |
| MS.gene059360 | MS.gene56132 | 0.812942 | 3.29E-51 | -1.69E-46 |
| MS.gene060084 | MS.gene56132 | 0.804032 | 2.62E-49 | -1.69E-46 |
| MS.gene06010 | MS.gene56132 | 0.803857 | 2.85E-49 | -1.69E-46 |
| MS.gene06011 | MS.gene56132 | 0.806913 | 6.53E-50 | -1.69E-46 |
| MS.gene060297 | MS.gene56132 | 0.846273 | 2.41E-59 | -1.69E-46 |
| MS.gene060869 | MS.gene56132 | 0.800049 | 1.73E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56132.t1 | MTR_7g022230 | 95.640 | 367 | 16 | 0 | 1 | 367 | 1 | 367 | 0.0 | 717 |
| MS.gene56132.t1 | MTR_3g082850 | 25.316 | 237 | 165 | 5 | 1 | 232 | 5 | 234 | 7.11e-16 | 78.2 |
| MS.gene56132.t1 | MTR_3g082850 | 25.316 | 237 | 165 | 5 | 1 | 232 | 5 | 234 | 7.16e-16 | 78.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56132.t1 | AT1G24265 | 40.149 | 269 | 152 | 6 | 1 | 264 | 1 | 265 | 5.32e-58 | 192 |
| MS.gene56132.t1 | AT1G24265 | 40.149 | 269 | 152 | 6 | 1 | 264 | 1 | 265 | 5.32e-58 | 192 |
| MS.gene56132.t1 | AT1G24267 | 34.877 | 367 | 199 | 10 | 1 | 359 | 1 | 335 | 1.48e-52 | 177 |
| MS.gene56132.t1 | AT1G24267 | 35.054 | 368 | 198 | 11 | 1 | 359 | 1 | 336 | 9.64e-52 | 176 |
| MS.gene56132.t1 | AT1G24265 | 35.197 | 304 | 153 | 7 | 1 | 264 | 1 | 300 | 3.15e-50 | 173 |
| MS.gene56132.t1 | AT1G04960 | 25.862 | 232 | 155 | 7 | 1 | 223 | 1 | 224 | 1.08e-14 | 74.7 |
| MS.gene56132.t1 | AT1G04960 | 23.810 | 210 | 148 | 5 | 19 | 223 | 39 | 241 | 2.10e-11 | 64.7 |
Find 99 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTAGTTCTGGTGGCTTTCT+TGG | 0.198980 | 7.4:+79680325 | MS.gene56132:CDS |
| TTGAAAGTTACCCTTGATAT+TGG | 0.285044 | 7.4:-79679680 | None:intergenic |
| TATGCAGCAGTGGTCATTAT+TGG | 0.289388 | 7.4:+79675115 | MS.gene56132:CDS |
| GATAAAATCACTTTAATAGA+AGG | 0.293332 | 7.4:+79678985 | MS.gene56132:CDS |
| ATGCAGCAGTGGTCATTATT+GGG | 0.324473 | 7.4:+79675116 | MS.gene56132:CDS |
| CTAGTTCTGGTGGCTTTCTT+GGG | 0.336790 | 7.4:+79680326 | MS.gene56132:CDS |
| CACCAGTTTCACCATCTTCC+AGG | 0.340313 | 7.4:+79679378 | MS.gene56132:CDS |
| GAAGGTTTATGAATCAATTC+AGG | 0.342315 | 7.4:+79676202 | MS.gene56132:CDS |
| CTTTGCAGTCAGGACCTTCC+AGG | 0.349606 | 7.4:+79679630 | MS.gene56132:CDS |
| TCTTGTATCTGGTACTTTCA+AGG | 0.361589 | 7.4:+79671465 | MS.gene56132:CDS |
| CGGTACTGTTATTAACTACT+TGG | 0.365927 | 7.4:-79680424 | None:intergenic |
| GGGTAGGATGTGGATGCCTT+TGG | 0.375016 | 7.4:+79675137 | MS.gene56132:CDS |
| AGATCTCTCTCATCAGATAT+TGG | 0.376614 | 7.4:-79680244 | None:intergenic |
| CAACGCGACACAGAGGAAAT+AGG | 0.385057 | 7.4:+79678837 | MS.gene56132:CDS |
| TCCCTTCACTCTATGATTTG+TGG | 0.393347 | 7.4:-79679131 | None:intergenic |
| CATCGAATCCACACGACTAC+TGG | 0.402244 | 7.4:-79680269 | None:intergenic |
| CACTTTAATAGAAGGGAATC+AGG | 0.402610 | 7.4:+79678993 | MS.gene56132:CDS |
| GCAAATGACTTACAATAGTT+CGG | 0.404220 | 7.4:-79678890 | None:intergenic |
| ATTGTAAATCCAAGTGGAAC+AGG | 0.409925 | 7.4:+79674871 | MS.gene56132:CDS |
| CTTGATATTGGTGTAATAGA+AGG | 0.417272 | 7.4:-79679668 | None:intergenic |
| CTCTACTCCACAGTATATTC+AGG | 0.422270 | 7.4:+79679186 | MS.gene56132:CDS |
| CTGAGAACGCGCAGTGTGAC+AGG | 0.423830 | 7.4:+79680379 | MS.gene56132:CDS |
| ATTATACAGGCTTTGCAGTC+AGG | 0.424003 | 7.4:+79679620 | MS.gene56132:intron |
| CAGGAAGCCGAGGGGAAGAT+AGG | 0.428041 | 7.4:+79678661 | MS.gene56132:intron |
| TAGGATGTGGATGCCTTTGG+TGG | 0.442173 | 7.4:+79675140 | MS.gene56132:CDS |
| CAATGATGCGTTGCTTGATC+AGG | 0.444499 | 7.4:+79673455 | MS.gene56132:CDS |
| AAGATAGGTGGAAGAATTAT+CGG | 0.446531 | 7.4:+79678676 | MS.gene56132:CDS |
| ACCCTGGAAGATGGTGAAAC+TGG | 0.449403 | 7.4:-79679380 | None:intergenic |
| AAGATCAATGAATCTAGTTC+TGG | 0.452565 | 7.4:+79680313 | MS.gene56132:CDS |
| CTTCTATTACACCAATATCA+AGG | 0.467188 | 7.4:+79679669 | MS.gene56132:CDS |
| GTCATTATTGGGGTAGGATG+TGG | 0.469037 | 7.4:+79675127 | MS.gene56132:CDS |
| CTGTTATTAACTACTTGGTC+GGG | 0.471803 | 7.4:-79680419 | None:intergenic |
| TCAGATCCGACAGTATTTGA+TGG | 0.482692 | 7.4:-79679820 | None:intergenic |
| TCATTGATCTTGTTTCCTGC+AGG | 0.483863 | 7.4:-79680301 | None:intergenic |
| TTGATCTTGTTTCCTGCAGG+AGG | 0.484112 | 7.4:-79680298 | None:intergenic |
| ACTGTTATTAACTACTTGGT+CGG | 0.485355 | 7.4:-79680420 | None:intergenic |
| GATCACAGGTTAGTAACCTT+CGG | 0.487093 | 7.4:+79674806 | MS.gene56132:intron |
| GATCTTGTTTCCTGCAGGAG+GGG | 0.487459 | 7.4:-79680296 | None:intergenic |
| AGTTTAAATGATGCTTGTAC+AGG | 0.492731 | 7.4:+79676161 | MS.gene56132:CDS |
| TGATCTTGTTTCCTGCAGGA+GGG | 0.500746 | 7.4:-79680297 | None:intergenic |
| AATGATGCTTGTACAGGCAT+AGG | 0.503064 | 7.4:+79676167 | MS.gene56132:CDS |
| TGGTAGCTCAATGGCTGGCA+TGG | 0.504873 | 7.4:-79679357 | None:intergenic |
| TACAGGCATAGGTAAACAGC+TGG | 0.507727 | 7.4:+79676178 | MS.gene56132:CDS |
| CTGGATCTGTGCCTCCAACT+AGG | 0.508414 | 7.4:+79679779 | MS.gene56132:CDS |
| TAACCTTCGGAAGCAAATAG+AGG | 0.516381 | 7.4:+79674819 | MS.gene56132:CDS |
| TCTTCCACCTATCTTCCCCT+CGG | 0.518512 | 7.4:-79678668 | None:intergenic |
| AGGCTGCTCAAGATATAGCC+TGG | 0.521316 | 7.4:-79679648 | None:intergenic |
| TGTACCTGCGCCGAGGAGAA+TGG | 0.523625 | 7.4:-79671149 | None:intergenic |
| GGCTTCTAAAGAAGGTGGAT+TGG | 0.524365 | 7.4:+79671429 | MS.gene56132:CDS |
| GATCCTCTATTTGCTTCCGA+AGG | 0.525550 | 7.4:-79674822 | None:intergenic |
| CACACCATGGCTCTCTCACT+CGG | 0.526772 | 7.4:+79671118 | None:intergenic |
| GGTGGATCAGCTGACCTAGT+TGG | 0.532601 | 7.4:-79679793 | None:intergenic |
| GGAGCAATGGCTTCTAAAGA+AGG | 0.537633 | 7.4:+79671421 | MS.gene56132:CDS |
| ACCAGTTTCACCATCTTCCA+GGG | 0.538108 | 7.4:+79679379 | MS.gene56132:CDS |
| TAAGCATAACCTGTTCCACT+TGG | 0.542443 | 7.4:-79674880 | None:intergenic |
| TCGATGAAAACCCCTCCTGC+AGG | 0.547660 | 7.4:+79680286 | MS.gene56132:CDS |
| AAAACGCTTACCCTGGAAGA+TGG | 0.549999 | 7.4:-79679389 | None:intergenic |
| AAATACTGTCGGATCTGACC+AGG | 0.554979 | 7.4:+79679825 | MS.gene56132:CDS |
| CTCATATGATGTTTGCAACA+AGG | 0.557317 | 7.4:+79676135 | MS.gene56132:CDS |
| ATAAAATCACTTTAATAGAA+GGG | 0.558423 | 7.4:+79678986 | MS.gene56132:CDS |
| CTTACCGAGAGTTTGCAGAA+TGG | 0.561316 | 7.4:+79679163 | MS.gene56132:CDS |
| CCGACAGTATTTGATGGAGA+TGG | 0.566249 | 7.4:-79679814 | None:intergenic |
| CTCACCATTCTCCTCGGCGC+AGG | 0.567185 | 7.4:+79671145 | MS.gene56132:CDS |
| TGCTCAAGATATAGCCTGGA+AGG | 0.567858 | 7.4:-79679644 | None:intergenic |
| ATCATTATTGTAAATCCAAG+TGG | 0.576815 | 7.4:+79674865 | MS.gene56132:CDS |
| CTTGTTGCAAACATCATATG+AGG | 0.577200 | 7.4:-79676134 | None:intergenic |
| ATCAATGAATCTAGTTCTGG+TGG | 0.578012 | 7.4:+79680316 | MS.gene56132:CDS |
| GGATCAGCTGACCTAGTTGG+AGG | 0.578994 | 7.4:-79679790 | None:intergenic |
| TGCCACAAATCATAGAGTGA+AGG | 0.579848 | 7.4:+79679129 | MS.gene56132:CDS |
| AGAGCCATTCTGCAAACTCT+CGG | 0.583473 | 7.4:-79679167 | None:intergenic |
| GCCACAAATCATAGAGTGAA+GGG | 0.587625 | 7.4:+79679130 | MS.gene56132:CDS |
| GCAGTGGTCATTATTGGGGT+AGG | 0.588721 | 7.4:+79675121 | MS.gene56132:CDS |
| GAAGCCGAGGGGAAGATAGG+TGG | 0.596262 | 7.4:+79678664 | MS.gene56132:CDS |
| CATAGGTAAACAGCTGGGGA+AGG | 0.596495 | 7.4:+79676184 | MS.gene56132:CDS |
| TTCTATTACACCAATATCAA+GGG | 0.598085 | 7.4:+79679670 | MS.gene56132:CDS |
| ATGTGGATGCCTTTGGTGGA+AGG | 0.598433 | 7.4:+79675144 | MS.gene56132:CDS |
| TGATGATTTCAGGAAGCCGA+GGG | 0.598749 | 7.4:+79678652 | MS.gene56132:intron |
| TGAAACTGGTGGTAGCTCAA+TGG | 0.608807 | 7.4:-79679366 | None:intergenic |
| AATGCCCTACCTTCCACCAA+AGG | 0.612201 | 7.4:-79675153 | None:intergenic |
| ACACTGCGCGTTCTCAGCAA+CGG | 0.612630 | 7.4:-79680373 | None:intergenic |
| ACAGTATTTGATGGAGATGG+TGG | 0.613685 | 7.4:-79679811 | None:intergenic |
| TGCAGCAGTGGTCATTATTG+GGG | 0.615945 | 7.4:+79675117 | MS.gene56132:CDS |
| AGGAAGAAAATATGCAGCAG+TGG | 0.626274 | 7.4:+79675105 | MS.gene56132:CDS |
| ACAGGCATAGGTAAACAGCT+GGG | 0.626357 | 7.4:+79676179 | MS.gene56132:CDS |
| GTGATGATTTCAGGAAGCCG+AGG | 0.627990 | 7.4:+79678651 | MS.gene56132:intron |
| CCATCTCCATCAAATACTGT+CGG | 0.628130 | 7.4:+79679814 | MS.gene56132:CDS |
| ACTGGTGGTAGCTCAATGGC+TGG | 0.629514 | 7.4:-79679362 | None:intergenic |
| GCAATGGCTTCTAAAGAAGG+TGG | 0.631408 | 7.4:+79671424 | MS.gene56132:CDS |
| AGATCTCTCCAGTAGTCGTG+TGG | 0.635591 | 7.4:+79680261 | MS.gene56132:CDS |
| GAGAGAGTGTACCTGCGCCG+AGG | 0.645397 | 7.4:-79671156 | None:intergenic |
| CGCGACACAGAGGAAATAGG+TGG | 0.657494 | 7.4:+79678840 | MS.gene56132:CDS |
| GGAAAGCTCACCATTCTCCT+CGG | 0.658989 | 7.4:+79671139 | MS.gene56132:CDS |
| TCAAGCAACGCATCATTGTG+TGG | 0.663243 | 7.4:-79673450 | None:intergenic |
| CAGGCATAGGTAAACAGCTG+GGG | 0.669956 | 7.4:+79676180 | MS.gene56132:CDS |
| CTTTCCGAGTGAGAGAGCCA+TGG | 0.684415 | 7.4:-79671122 | None:intergenic |
| TGCAATACAACGCGACACAG+AGG | 0.694660 | 7.4:+79678830 | MS.gene56132:CDS |
| CTGGAAGATGGTGAAACTGG+TGG | 0.738406 | 7.4:-79679377 | None:intergenic |
| GTTCATGCCTGAATATACTG+TGG | 0.744829 | 7.4:-79679193 | None:intergenic |
| GATGATTTCAGGAAGCCGAG+GGG | 0.766630 | 7.4:+79678653 | MS.gene56132:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 79671124 | 79680435 | 79671124 | ID=MS.gene56132 |
| chr7.4 | mRNA | 79671124 | 79680435 | 79671124 | ID=MS.gene56132.t1;Parent=MS.gene56132 |
| chr7.4 | exon | 79671124 | 79671166 | 79671124 | ID=MS.gene56132.t1.exon1;Parent=MS.gene56132.t1 |
| chr7.4 | CDS | 79671124 | 79671166 | 79671124 | ID=cds.MS.gene56132.t1;Parent=MS.gene56132.t1 |
| chr7.4 | exon | 79671410 | 79671486 | 79671410 | ID=MS.gene56132.t1.exon2;Parent=MS.gene56132.t1 |
| chr7.4 | CDS | 79671410 | 79671486 | 79671410 | ID=cds.MS.gene56132.t1;Parent=MS.gene56132.t1 |
| chr7.4 | exon | 79673399 | 79673476 | 79673399 | ID=MS.gene56132.t1.exon3;Parent=MS.gene56132.t1 |
| chr7.4 | CDS | 79673399 | 79673476 | 79673399 | ID=cds.MS.gene56132.t1;Parent=MS.gene56132.t1 |
| chr7.4 | exon | 79674814 | 79674892 | 79674814 | ID=MS.gene56132.t1.exon4;Parent=MS.gene56132.t1 |
| chr7.4 | CDS | 79674814 | 79674892 | 79674814 | ID=cds.MS.gene56132.t1;Parent=MS.gene56132.t1 |
| chr7.4 | exon | 79675104 | 79675165 | 79675104 | ID=MS.gene56132.t1.exon5;Parent=MS.gene56132.t1 |
| chr7.4 | CDS | 79675104 | 79675165 | 79675104 | ID=cds.MS.gene56132.t1;Parent=MS.gene56132.t1 |
| chr7.4 | exon | 79676125 | 79676223 | 79676125 | ID=MS.gene56132.t1.exon6;Parent=MS.gene56132.t1 |
| chr7.4 | CDS | 79676125 | 79676223 | 79676125 | ID=cds.MS.gene56132.t1;Parent=MS.gene56132.t1 |
| chr7.4 | exon | 79678664 | 79678753 | 79678664 | ID=MS.gene56132.t1.exon7;Parent=MS.gene56132.t1 |
| chr7.4 | CDS | 79678664 | 79678753 | 79678664 | ID=cds.MS.gene56132.t1;Parent=MS.gene56132.t1 |
| chr7.4 | exon | 79678825 | 79678899 | 79678825 | ID=MS.gene56132.t1.exon8;Parent=MS.gene56132.t1 |
| chr7.4 | CDS | 79678825 | 79678899 | 79678825 | ID=cds.MS.gene56132.t1;Parent=MS.gene56132.t1 |
| chr7.4 | exon | 79678982 | 79679014 | 79678982 | ID=MS.gene56132.t1.exon9;Parent=MS.gene56132.t1 |
| chr7.4 | CDS | 79678982 | 79679014 | 79678982 | ID=cds.MS.gene56132.t1;Parent=MS.gene56132.t1 |
| chr7.4 | exon | 79679127 | 79679207 | 79679127 | ID=MS.gene56132.t1.exon10;Parent=MS.gene56132.t1 |
| chr7.4 | CDS | 79679127 | 79679207 | 79679127 | ID=cds.MS.gene56132.t1;Parent=MS.gene56132.t1 |
| chr7.4 | exon | 79679347 | 79679400 | 79679347 | ID=MS.gene56132.t1.exon11;Parent=MS.gene56132.t1 |
| chr7.4 | CDS | 79679347 | 79679400 | 79679347 | ID=cds.MS.gene56132.t1;Parent=MS.gene56132.t1 |
| chr7.4 | exon | 79679629 | 79679691 | 79679629 | ID=MS.gene56132.t1.exon12;Parent=MS.gene56132.t1 |
| chr7.4 | CDS | 79679629 | 79679691 | 79679629 | ID=cds.MS.gene56132.t1;Parent=MS.gene56132.t1 |
| chr7.4 | exon | 79679778 | 79679846 | 79679778 | ID=MS.gene56132.t1.exon13;Parent=MS.gene56132.t1 |
| chr7.4 | CDS | 79679778 | 79679846 | 79679778 | ID=cds.MS.gene56132.t1;Parent=MS.gene56132.t1 |
| chr7.4 | exon | 79680235 | 79680435 | 79680235 | ID=MS.gene56132.t1.exon14;Parent=MS.gene56132.t1 |
| chr7.4 | CDS | 79680235 | 79680435 | 79680235 | ID=cds.MS.gene56132.t1;Parent=MS.gene56132.t1 |
| Gene Sequence |
| Protein sequence |
