Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58081.t1 | XP_003610303.2 | 96.2 | 157 | 6 | 0 | 1 | 157 | 105 | 261 | 2.00E-76 | 295 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58081.t1 | G7JFL7 | 96.2 | 157 | 6 | 0 | 1 | 157 | 105 | 261 | 1.4e-76 | 295.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene58081 | 0.837859 | 4.05E-57 | -1.69E-46 |
| MS.gene04920 | MS.gene58081 | 0.815302 | 9.91E-52 | -1.69E-46 |
| MS.gene049283 | MS.gene58081 | 0.806062 | 9.87E-50 | -1.69E-46 |
| MS.gene049343 | MS.gene58081 | 0.800539 | 1.37E-48 | -1.69E-46 |
| MS.gene049499 | MS.gene58081 | 0.820011 | 8.59E-53 | -1.69E-46 |
| MS.gene049680 | MS.gene58081 | 0.829322 | 5.49E-55 | -1.69E-46 |
| MS.gene049983 | MS.gene58081 | 0.838708 | 2.45E-57 | -1.69E-46 |
| MS.gene050081 | MS.gene58081 | 0.809205 | 2.12E-50 | -1.69E-46 |
| MS.gene050737 | MS.gene58081 | 0.815055 | 1.12E-51 | -1.69E-46 |
| MS.gene050941 | MS.gene58081 | 0.809636 | 1.72E-50 | -1.69E-46 |
| MS.gene051975 | MS.gene58081 | 0.806842 | 6.76E-50 | -1.69E-46 |
| MS.gene052518 | MS.gene58081 | 0.856881 | 2.38E-62 | -1.69E-46 |
| MS.gene052519 | MS.gene58081 | 0.803355 | 3.63E-49 | -1.69E-46 |
| MS.gene052685 | MS.gene58081 | 0.81041 | 1.17E-50 | -1.69E-46 |
| MS.gene053228 | MS.gene58081 | 0.814436 | 1.54E-51 | -1.69E-46 |
| MS.gene053298 | MS.gene58081 | 0.823696 | 1.21E-53 | -1.69E-46 |
| MS.gene053963 | MS.gene58081 | 0.817628 | 2.99E-52 | -1.69E-46 |
| MS.gene05458 | MS.gene58081 | 0.814355 | 1.61E-51 | -1.69E-46 |
| MS.gene055150 | MS.gene58081 | 0.800935 | 1.14E-48 | -1.69E-46 |
| MS.gene055801 | MS.gene58081 | 0.803886 | 2.81E-49 | -1.69E-46 |
| MS.gene056010 | MS.gene58081 | 0.804848 | 1.77E-49 | -1.69E-46 |
| MS.gene056699 | MS.gene58081 | 0.815431 | 9.28E-52 | -1.69E-46 |
| MS.gene056986 | MS.gene58081 | 0.815372 | 9.56E-52 | -1.69E-46 |
| MS.gene057102 | MS.gene58081 | 0.840088 | 1.07E-57 | -1.69E-46 |
| MS.gene057155 | MS.gene58081 | 0.82396 | 1.05E-53 | -1.69E-46 |
| MS.gene057208 | MS.gene58081 | 0.811765 | 5.94E-51 | -1.69E-46 |
| MS.gene058592 | MS.gene58081 | 0.800774 | 1.23E-48 | -1.69E-46 |
| MS.gene058783 | MS.gene58081 | 0.806982 | 6.32E-50 | -1.69E-46 |
| MS.gene05952 | MS.gene58081 | 0.817192 | 3.75E-52 | -1.69E-46 |
| MS.gene05985 | MS.gene58081 | -0.826831 | 2.19E-54 | -1.69E-46 |
| MS.gene059900 | MS.gene58081 | -0.805817 | 1.11E-49 | -1.69E-46 |
| MS.gene059901 | MS.gene58081 | -0.814074 | 1.85E-51 | -1.69E-46 |
| MS.gene06011 | MS.gene58081 | 0.818345 | 2.06E-52 | -1.69E-46 |
| MS.gene060338 | MS.gene58081 | 0.83477 | 2.47E-56 | -1.69E-46 |
| MS.gene060932 | MS.gene58081 | 0.80178 | 7.66E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene58081 | 0.854495 | 1.18E-61 | -1.69E-46 |
| MS.gene060937 | MS.gene58081 | 0.856555 | 2.97E-62 | -1.69E-46 |
| MS.gene061188 | MS.gene58081 | 0.811348 | 7.32E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 40 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATTGAAAACTCTTTCTTT+TGG | 0.207441 | 8.4:-3744024 | None:intergenic |
| GGCAAGACCAAGTGGCATTA+AGG | 0.254676 | 8.4:+3744796 | MS.gene58081:CDS |
| TACTTAAGCTCCTACCATTC+TGG | 0.258384 | 8.4:-3744098 | None:intergenic |
| CACTTTGAACCCTATCTTTG+TGG | 0.295669 | 8.4:-3744170 | None:intergenic |
| TAATATTAATTACCGAAATC+TGG | 0.317567 | 8.4:-3744239 | None:intergenic |
| CTTCGACTTGAACCAGATTT+CGG | 0.333900 | 8.4:+3744227 | MS.gene58081:CDS |
| GAATGGAGTTACACTCTTCT+TGG | 0.337402 | 8.4:-3744205 | None:intergenic |
| ACAGAGAGAAGAGGAGGAAT+TGG | 0.341758 | 8.4:+3744626 | MS.gene58081:intron |
| GCAAGACCAAGTGGCATTAA+GGG | 0.386403 | 8.4:+3744797 | MS.gene58081:CDS |
| TTGACTCCAACCACAAAGAT+AGG | 0.410742 | 8.4:+3744160 | MS.gene58081:CDS |
| TTGAACCCTATCTTTGTGGT+TGG | 0.454353 | 8.4:-3744166 | None:intergenic |
| CTTTCAGTTTGTAGAAACAT+TGG | 0.470205 | 8.4:+3744735 | MS.gene58081:CDS |
| TTCAATTTAGAAGCGGTATG+AGG | 0.471293 | 8.4:-3744000 | None:intergenic |
| ATCTGGTTCAAGTCGAAGAA+TGG | 0.477680 | 8.4:-3744222 | None:intergenic |
| GATCAAGTAGGGCGGGGAAA+AGG | 0.486093 | 8.4:+3744763 | MS.gene58081:CDS |
| GGGAAGGAGTTACAATAGAA+AGG | 0.520551 | 8.4:+3743963 | None:intergenic |
| GGAGAAGGATCAAGTAGGGC+GGG | 0.526648 | 8.4:+3744756 | MS.gene58081:CDS |
| GTTCTTCAATCCACATGGAC+TGG | 0.526988 | 8.4:-3744659 | None:intergenic |
| TGGAGTTACACTCTTCTTGG+CGG | 0.529993 | 8.4:-3744202 | None:intergenic |
| GTTTGTAGAAACATTGGAGA+AGG | 0.531380 | 8.4:+3744741 | MS.gene58081:CDS |
| GTGTTTGACTTAAACCAGAA+TGG | 0.533639 | 8.4:+3744084 | MS.gene58081:CDS |
| GAGAGAAGAGGAGGAATTGG+AGG | 0.534033 | 8.4:+3744629 | MS.gene58081:intron |
| TTGTGGTTGGAGTCAAGCAC+AGG | 0.534806 | 8.4:-3744153 | None:intergenic |
| ATCAAACCCTTAATGCCACT+TGG | 0.560678 | 8.4:-3744803 | None:intergenic |
| TGTTGATTCACAGAGAGAAG+AGG | 0.561535 | 8.4:+3744617 | MS.gene58081:intron |
| CGGGGAAAAGGAAGATTACA+TGG | 0.572207 | 8.4:+3744775 | MS.gene58081:CDS |
| AGAGAAGAGGAGGAATTGGA+GGG | 0.574281 | 8.4:+3744630 | MS.gene58081:CDS |
| CATTGGAGAAGGATCAAGTA+GGG | 0.576097 | 8.4:+3744752 | MS.gene58081:CDS |
| TTGACTTAAACCAGAATGGT+AGG | 0.579603 | 8.4:+3744088 | MS.gene58081:CDS |
| TGATTCACAGAGAGAAGAGG+AGG | 0.590324 | 8.4:+3744620 | MS.gene58081:intron |
| AGATAGGGTTCAAAGTGGAA+AGG | 0.601203 | 8.4:+3744176 | MS.gene58081:CDS |
| TGACTCCAACCACAAAGATA+GGG | 0.608165 | 8.4:+3744161 | MS.gene58081:CDS |
| CACAAAGATAGGGTTCAAAG+TGG | 0.610186 | 8.4:+3744171 | MS.gene58081:CDS |
| GAGAAGGATCAAGTAGGGCG+GGG | 0.648300 | 8.4:+3744757 | MS.gene58081:CDS |
| ACATTGGAGAAGGATCAAGT+AGG | 0.659795 | 8.4:+3744751 | MS.gene58081:CDS |
| TGGAGAAGGATCAAGTAGGG+CGG | 0.673940 | 8.4:+3744755 | MS.gene58081:CDS |
| GATTACATGGCAAGACCAAG+TGG | 0.685084 | 8.4:+3744788 | MS.gene58081:CDS |
| GAAAGCTTGATATCGTTGTG+CGG | 0.687388 | 8.4:-3744717 | None:intergenic |
| AGGGAGATAACCAGTCCATG+TGG | 0.690510 | 8.4:+3744649 | MS.gene58081:CDS |
| TCTGGTTTAAGTCAAACACA+TGG | 0.703585 | 8.4:-3744080 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAATCAAAAAACACAAAAA+AGG | - | chr8.4:3744299-3744318 | None:intergenic | 20.0% |
| !! | TAATATTAATTACCGAAATC+TGG | - | chr8.4:3744242-3744261 | None:intergenic | 20.0% |
| !!! | AAAATCGATGAAAAGTATTA+AGG | - | chr8.4:3744598-3744617 | None:intergenic | 20.0% |
| !!! | AAATCGATGAAAAGTATTAA+GGG | - | chr8.4:3744597-3744616 | None:intergenic | 20.0% |
| !!! | GTATGTTTAAAATGACTTAT+TGG | - | chr8.4:3744575-3744594 | None:intergenic | 20.0% |
| ! | ATTAAGATGTTTATCCAAAC+AGG | - | chr8.4:3744335-3744354 | None:intergenic | 25.0% |
| !! | ATTTTATAAGCTCTATGTGA+TGG | - | chr8.4:3744467-3744486 | None:intergenic | 25.0% |
| !! | CATTGAAAACTCTTTCTTTT+GGG | - | chr8.4:3744026-3744045 | None:intergenic | 25.0% |
| !!! | TTTTTGATTCTAGTAGACTT+GGG | + | chr8.4:3744308-3744327 | MS.gene58081:intron | 25.0% |
| !!! | TTTTTTGATTCTAGTAGACT+TGG | + | chr8.4:3744307-3744326 | MS.gene58081:intron | 25.0% |
| ACTTATTGGAGTTTATCTAC+TGG | - | chr8.4:3744561-3744580 | None:intergenic | 30.0% | |
| CTTTCAGTTTGTAGAAACAT+TGG | + | chr8.4:3744735-3744754 | MS.gene58081:CDS | 30.0% | |
| TAGCATAATCACACATCATA+TGG | + | chr8.4:3744365-3744384 | MS.gene58081:intron | 30.0% | |
| ! | AAAGAAAGAGTTTTCAATGG+AGG | + | chr8.4:3744027-3744046 | MS.gene58081:CDS | 30.0% |
| ! | CCAAAAGAAAGAGTTTTCAA+TGG | + | chr8.4:3744024-3744043 | MS.gene58081:CDS | 30.0% |
| ! | CCATTGAAAACTCTTTCTTT+TGG | - | chr8.4:3744027-3744046 | None:intergenic | 30.0% |
| !!! | TTTTGGGTTCAATTTAGAAG+CGG | - | chr8.4:3744010-3744029 | None:intergenic | 30.0% |
| GTTTGTAGAAACATTGGAGA+AGG | + | chr8.4:3744741-3744760 | MS.gene58081:CDS | 35.0% | |
| TTCAATTTAGAAGCGGTATG+AGG | - | chr8.4:3744003-3744022 | None:intergenic | 35.0% | |
| TTGACTTAAACCAGAATGGT+AGG | + | chr8.4:3744088-3744107 | MS.gene58081:CDS | 35.0% | |
| ! | CTTTTGTTCTTCAATCCACA+TGG | - | chr8.4:3744667-3744686 | None:intergenic | 35.0% |
| ! | GTGTTTGACTTAAACCAGAA+TGG | + | chr8.4:3744084-3744103 | MS.gene58081:CDS | 35.0% |
| ! | TCTGGTTTAAGTCAAACACA+TGG | - | chr8.4:3744083-3744102 | None:intergenic | 35.0% |
| ACATTGGAGAAGGATCAAGT+AGG | + | chr8.4:3744751-3744770 | MS.gene58081:CDS | 40.0% | |
| AGATAGGGTTCAAAGTGGAA+AGG | + | chr8.4:3744176-3744195 | MS.gene58081:CDS | 40.0% | |
| CACAAAGATAGGGTTCAAAG+TGG | + | chr8.4:3744171-3744190 | MS.gene58081:CDS | 40.0% | |
| CACTTTGAACCCTATCTTTG+TGG | - | chr8.4:3744173-3744192 | None:intergenic | 40.0% | |
| CATTGGAGAAGGATCAAGTA+GGG | + | chr8.4:3744752-3744771 | MS.gene58081:CDS | 40.0% | |
| GAAAGCTTGATATCGTTGTG+CGG | - | chr8.4:3744720-3744739 | None:intergenic | 40.0% | |
| GAATGGAGTTACACTCTTCT+TGG | - | chr8.4:3744208-3744227 | None:intergenic | 40.0% | |
| TACTTAAGCTCCTACCATTC+TGG | - | chr8.4:3744101-3744120 | None:intergenic | 40.0% | |
| TGACTCCAACCACAAAGATA+GGG | + | chr8.4:3744161-3744180 | MS.gene58081:CDS | 40.0% | |
| TTGAACCCTATCTTTGTGGT+TGG | - | chr8.4:3744169-3744188 | None:intergenic | 40.0% | |
| TTGACTCCAACCACAAAGAT+AGG | + | chr8.4:3744160-3744179 | MS.gene58081:CDS | 40.0% | |
| ! | ATCTGGTTCAAGTCGAAGAA+TGG | - | chr8.4:3744225-3744244 | None:intergenic | 40.0% |
| ! | CTTCGACTTGAACCAGATTT+CGG | + | chr8.4:3744227-3744246 | MS.gene58081:CDS | 40.0% |
| !! | TGTTGATTCACAGAGAGAAG+AGG | + | chr8.4:3744617-3744636 | MS.gene58081:intron | 40.0% |
| ACAGAGAGAAGAGGAGGAAT+TGG | + | chr8.4:3744626-3744645 | MS.gene58081:intron | 45.0% | |
| AGAGAAGAGGAGGAATTGGA+GGG | + | chr8.4:3744630-3744649 | MS.gene58081:CDS | 45.0% | |
| CGGGGAAAAGGAAGATTACA+TGG | + | chr8.4:3744775-3744794 | MS.gene58081:CDS | 45.0% | |
| GATTACATGGCAAGACCAAG+TGG | + | chr8.4:3744788-3744807 | MS.gene58081:CDS | 45.0% | |
| GCAAGACCAAGTGGCATTAA+GGG | + | chr8.4:3744797-3744816 | MS.gene58081:CDS | 45.0% | |
| GTTCTTCAATCCACATGGAC+TGG | - | chr8.4:3744662-3744681 | None:intergenic | 45.0% | |
| TAGTAGACTTGGGTCCTGTT+TGG | + | chr8.4:3744318-3744337 | MS.gene58081:intron | 45.0% | |
| TGATTCACAGAGAGAAGAGG+AGG | + | chr8.4:3744620-3744639 | MS.gene58081:intron | 45.0% | |
| TGGAGTTACACTCTTCTTGG+CGG | - | chr8.4:3744205-3744224 | None:intergenic | 45.0% | |
| AGGGAGATAACCAGTCCATG+TGG | + | chr8.4:3744649-3744668 | MS.gene58081:CDS | 50.0% | |
| GAGAGAAGAGGAGGAATTGG+AGG | + | chr8.4:3744629-3744648 | MS.gene58081:intron | 50.0% | |
| GGCAAGACCAAGTGGCATTA+AGG | + | chr8.4:3744796-3744815 | MS.gene58081:CDS | 50.0% | |
| TGGAGAAGGATCAAGTAGGG+CGG | + | chr8.4:3744755-3744774 | MS.gene58081:CDS | 50.0% | |
| TTGTGGTTGGAGTCAAGCAC+AGG | - | chr8.4:3744156-3744175 | None:intergenic | 50.0% | |
| GAGAAGGATCAAGTAGGGCG+GGG | + | chr8.4:3744757-3744776 | MS.gene58081:CDS | 55.0% | |
| GATCAAGTAGGGCGGGGAAA+AGG | + | chr8.4:3744763-3744782 | MS.gene58081:CDS | 55.0% | |
| GGAGAAGGATCAAGTAGGGC+GGG | + | chr8.4:3744756-3744775 | MS.gene58081:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 3743969 | 3744824 | 3743969 | ID=MS.gene58081 |
| chr8.4 | mRNA | 3743969 | 3744824 | 3743969 | ID=MS.gene58081.t1;Parent=MS.gene58081 |
| chr8.4 | exon | 3743969 | 3744248 | 3743969 | ID=MS.gene58081.t1.exon1;Parent=MS.gene58081.t1 |
| chr8.4 | CDS | 3743969 | 3744248 | 3743969 | ID=cds.MS.gene58081.t1;Parent=MS.gene58081.t1 |
| chr8.4 | exon | 3744630 | 3744824 | 3744630 | ID=MS.gene58081.t1.exon2;Parent=MS.gene58081.t1 |
| chr8.4 | CDS | 3744630 | 3744824 | 3744630 | ID=cds.MS.gene58081.t1;Parent=MS.gene58081.t1 |
| Gene Sequence |
| Protein sequence |
