Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58980.t1 | PNX75878.1 | 73 | 63 | 17 | 0 | 1 | 63 | 28 | 90 | 3.30E-14 | 87 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58980.t1 | A0A2K3LBG3 | 73.0 | 63 | 17 | 0 | 1 | 63 | 28 | 90 | 2.4e-14 | 87.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049232 | MS.gene58980 | 0.836997 | 6.74E-57 | -1.69E-46 |
| MS.gene049396 | MS.gene58980 | 0.806074 | 9.81E-50 | -1.69E-46 |
| MS.gene049552 | MS.gene58980 | 0.804855 | 1.77E-49 | -1.69E-46 |
| MS.gene050170 | MS.gene58980 | 0.830194 | 3.37E-55 | -1.69E-46 |
| MS.gene050216 | MS.gene58980 | 0.801916 | 7.18E-49 | -1.69E-46 |
| MS.gene050323 | MS.gene58980 | 0.825363 | 4.88E-54 | -1.69E-46 |
| MS.gene050496 | MS.gene58980 | 0.841376 | 4.93E-58 | -1.69E-46 |
| MS.gene050502 | MS.gene58980 | 0.807993 | 3.85E-50 | -1.69E-46 |
| MS.gene050504 | MS.gene58980 | 0.804824 | 1.79E-49 | -1.69E-46 |
| MS.gene050675 | MS.gene58980 | 0.813286 | 2.76E-51 | -1.69E-46 |
| MS.gene050697 | MS.gene58980 | 0.822834 | 1.92E-53 | -1.69E-46 |
| MS.gene050868 | MS.gene58980 | 0.838113 | 3.49E-57 | -1.69E-46 |
| MS.gene050871 | MS.gene58980 | 0.841763 | 3.90E-58 | -1.69E-46 |
| MS.gene051509 | MS.gene58980 | 0.815704 | 8.07E-52 | -1.69E-46 |
| MS.gene051941 | MS.gene58980 | 0.825208 | 5.31E-54 | -1.69E-46 |
| MS.gene052164 | MS.gene58980 | 0.805345 | 1.40E-49 | -1.69E-46 |
| MS.gene052482 | MS.gene58980 | 0.802673 | 5.02E-49 | -1.69E-46 |
| MS.gene052486 | MS.gene58980 | 0.80505 | 1.61E-49 | -1.69E-46 |
| MS.gene053203 | MS.gene58980 | 0.801724 | 7.87E-49 | -1.69E-46 |
| MS.gene053420 | MS.gene58980 | -0.807624 | 4.62E-50 | -1.69E-46 |
| MS.gene053574 | MS.gene58980 | 0.810334 | 1.21E-50 | -1.69E-46 |
| MS.gene053626 | MS.gene58980 | 0.816904 | 4.35E-52 | -1.69E-46 |
| MS.gene053824 | MS.gene58980 | 0.801237 | 9.90E-49 | -1.69E-46 |
| MS.gene054595 | MS.gene58980 | 0.821579 | 3.75E-53 | -1.69E-46 |
| MS.gene054954 | MS.gene58980 | 0.800772 | 1.23E-48 | -1.69E-46 |
| MS.gene055502 | MS.gene58980 | 0.804514 | 2.08E-49 | -1.69E-46 |
| MS.gene05589 | MS.gene58980 | 0.815661 | 8.25E-52 | -1.69E-46 |
| MS.gene056166 | MS.gene58980 | 0.814763 | 1.31E-51 | -1.69E-46 |
| MS.gene056387 | MS.gene58980 | 0.81396 | 1.96E-51 | -1.69E-46 |
| MS.gene056504 | MS.gene58980 | 0.837707 | 4.43E-57 | -1.69E-46 |
| MS.gene057019 | MS.gene58980 | 0.802602 | 5.19E-49 | -1.69E-46 |
| MS.gene057076 | MS.gene58980 | 0.803203 | 3.90E-49 | -1.69E-46 |
| MS.gene057086 | MS.gene58980 | 0.818199 | 2.22E-52 | -1.69E-46 |
| MS.gene05787 | MS.gene58980 | 0.820179 | 7.86E-53 | -1.69E-46 |
| MS.gene058157 | MS.gene58980 | 0.822899 | 1.85E-53 | -1.69E-46 |
| MS.gene058171 | MS.gene58980 | -0.810996 | 8.73E-51 | -1.69E-46 |
| MS.gene058173 | MS.gene58980 | -0.81221 | 4.75E-51 | -1.69E-46 |
| MS.gene058175 | MS.gene58980 | -0.811622 | 6.39E-51 | -1.69E-46 |
| MS.gene05837 | MS.gene58980 | 0.81797 | 2.50E-52 | -1.69E-46 |
| MS.gene059367 | MS.gene58980 | 0.826957 | 2.04E-54 | -1.69E-46 |
| MS.gene059680 | MS.gene58980 | 0.802764 | 4.80E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene58980 | 0.825794 | 3.86E-54 | -1.69E-46 |
| MS.gene06000 | MS.gene58980 | 0.847617 | 1.03E-59 | -1.69E-46 |
| MS.gene060187 | MS.gene58980 | -0.804095 | 2.55E-49 | -1.69E-46 |
| MS.gene060593 | MS.gene58980 | -0.808035 | 3.77E-50 | -1.69E-46 |
| MS.gene060595 | MS.gene58980 | -0.830803 | 2.39E-55 | -1.69E-46 |
| MS.gene060778 | MS.gene58980 | 0.877919 | 4.27E-69 | -1.69E-46 |
| MS.gene06077 | MS.gene58980 | 0.807622 | 4.62E-50 | -1.69E-46 |
| MS.gene060982 | MS.gene58980 | 0.81105 | 8.50E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATAGCAGGGACACATTTCT+TGG | 0.264407 | 5.2:+39983602 | None:intergenic |
| GGTGGTTTGGTTGGCATAGC+AGG | 0.303830 | 5.2:+39983588 | None:intergenic |
| GGCACTTGCGTCTGCCTCCA+TGG | 0.330551 | 5.2:+39983566 | None:intergenic |
| TGCGTGCCACCAGGAACTTC+TGG | 0.331803 | 5.2:-39983628 | MS.gene58980:CDS |
| CACGGCCGCGCCTCTGCCAC+AGG | 0.395156 | 5.2:-39983681 | MS.gene58980:CDS |
| TCTTGGTTACCAGAAGTTCC+TGG | 0.400394 | 5.2:+39983619 | None:intergenic |
| GTCTGCCTCCATGGGTGGTT+TGG | 0.427775 | 5.2:+39983575 | None:intergenic |
| GCCTCCATGGGTGGTTTGGT+TGG | 0.435015 | 5.2:+39983579 | None:intergenic |
| TTCCACATGCCCTGTGGCAG+AGG | 0.454808 | 5.2:+39983671 | None:intergenic |
| GGCGCGGCCGTGATGATAAA+CGG | 0.465640 | 5.2:+39983692 | None:intergenic |
| CGCCTCTGCCACAGGGCATG+TGG | 0.480960 | 5.2:-39983673 | MS.gene58980:CDS |
| GCACTTGCGTCTGCCTCCAT+GGG | 0.498020 | 5.2:+39983567 | None:intergenic |
| CTTGCGTCTGCCTCCATGGG+TGG | 0.501890 | 5.2:+39983570 | None:intergenic |
| TGATGATTCAACTCAATTCA+AGG | 0.508747 | 5.2:+39983544 | None:intergenic |
| CGGTGCAACTGCGTGCCACC+AGG | 0.530335 | 5.2:-39983637 | MS.gene58980:CDS |
| GTGGTTTGGTTGGCATAGCA+GGG | 0.546195 | 5.2:+39983589 | None:intergenic |
| GCAATGGAGCATGTGCTGCT+AGG | 0.562822 | 5.2:-39983717 | MS.gene58980:CDS |
| ATATAAATTTCAGATTGCAA+TGG | 0.566444 | 5.2:-39983733 | None:intergenic |
| GATGATTCAACTCAATTCAA+GGG | 0.567646 | 5.2:+39983545 | None:intergenic |
| CATGCCCTGTGGCAGAGGCG+CGG | 0.598879 | 5.2:+39983676 | None:intergenic |
| CATGTGGAACTTGCTGCAGT+CGG | 0.613537 | 5.2:-39983657 | MS.gene58980:CDS |
| ACGGCCGCGCCTCTGCCACA+GGG | 0.616011 | 5.2:-39983680 | MS.gene58980:CDS |
| CTAGGTGCCGTTTATCATCA+CGG | 0.637583 | 5.2:-39983699 | MS.gene58980:CDS |
| TATGCCAACCAAACCACCCA+TGG | 0.640986 | 5.2:-39983583 | MS.gene58980:CDS |
| TGGTTACCAGAAGTTCCTGG+TGG | 0.663688 | 5.2:+39983622 | None:intergenic |
| GCCAACCAAACCACCCATGG+AGG | 0.699460 | 5.2:-39983580 | MS.gene58980:CDS |
| AGCAAGTTCCACATGCCCTG+TGG | 0.700479 | 5.2:+39983665 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CATAGCAGGGACACATTTCT+TGG | + | chr5.2:39983672-39983691 | None:intergenic | 45.0% | |
| TCTTGGTTACCAGAAGTTCC+TGG | + | chr5.2:39983655-39983674 | None:intergenic | 45.0% | |
| !! | CTAGGTGCCGTTTATCATCA+CGG | - | chr5.2:39983572-39983591 | MS.gene58980:CDS | 45.0% |
| TATGCCAACCAAACCACCCA+TGG | - | chr5.2:39983688-39983707 | MS.gene58980:CDS | 50.0% | |
| TGGTTACCAGAAGTTCCTGG+TGG | + | chr5.2:39983652-39983671 | None:intergenic | 50.0% | |
| !! | CATGTGGAACTTGCTGCAGT+CGG | - | chr5.2:39983614-39983633 | MS.gene58980:CDS | 50.0% |
| !! | GTGGTTTGGTTGGCATAGCA+GGG | + | chr5.2:39983685-39983704 | None:intergenic | 50.0% |
| AGCAAGTTCCACATGCCCTG+TGG | + | chr5.2:39983609-39983628 | None:intergenic | 55.0% | |
| ! | GCAATGGAGCATGTGCTGCT+AGG | - | chr5.2:39983554-39983573 | MS.gene58980:CDS | 55.0% |
| !! | GGTGGTTTGGTTGGCATAGC+AGG | + | chr5.2:39983686-39983705 | None:intergenic | 55.0% |
| GCACTTGCGTCTGCCTCCAT+GGG | + | chr5.2:39983707-39983726 | None:intergenic | 60.0% | |
| GCCAACCAAACCACCCATGG+AGG | - | chr5.2:39983691-39983710 | MS.gene58980:CDS | 60.0% | |
| GGCGCGGCCGTGATGATAAA+CGG | + | chr5.2:39983582-39983601 | None:intergenic | 60.0% | |
| GTCTGCCTCCATGGGTGGTT+TGG | + | chr5.2:39983699-39983718 | None:intergenic | 60.0% | |
| TGCGTGCCACCAGGAACTTC+TGG | - | chr5.2:39983643-39983662 | MS.gene58980:CDS | 60.0% | |
| TTCCACATGCCCTGTGGCAG+AGG | + | chr5.2:39983603-39983622 | None:intergenic | 60.0% | |
| !! | GCCTCCATGGGTGGTTTGGT+TGG | + | chr5.2:39983695-39983714 | None:intergenic | 60.0% |
| CTTGCGTCTGCCTCCATGGG+TGG | + | chr5.2:39983704-39983723 | None:intergenic | 65.0% | |
| GGCACTTGCGTCTGCCTCCA+TGG | + | chr5.2:39983708-39983727 | None:intergenic | 65.0% | |
| CATGCCCTGTGGCAGAGGCG+CGG | + | chr5.2:39983598-39983617 | None:intergenic | 70.0% | |
| CGCCTCTGCCACAGGGCATG+TGG | - | chr5.2:39983598-39983617 | MS.gene58980:CDS | 70.0% | |
| CGGTGCAACTGCGTGCCACC+AGG | - | chr5.2:39983634-39983653 | MS.gene58980:CDS | 70.0% | |
| ACGGCCGCGCCTCTGCCACA+GGG | - | chr5.2:39983591-39983610 | MS.gene58980:CDS | 75.0% | |
| ! | CACGGCCGCGCCTCTGCCAC+AGG | - | chr5.2:39983590-39983609 | MS.gene58980:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 39983551 | 39983742 | 39983551 | ID=MS.gene58980 |
| chr5.2 | mRNA | 39983551 | 39983742 | 39983551 | ID=MS.gene58980.t1;Parent=MS.gene58980 |
| chr5.2 | exon | 39983551 | 39983742 | 39983551 | ID=MS.gene58980.t1.exon1;Parent=MS.gene58980.t1 |
| chr5.2 | CDS | 39983551 | 39983742 | 39983551 | ID=cds.MS.gene58980.t1;Parent=MS.gene58980.t1 |
| Gene Sequence |
| Protein sequence |
