Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60021.t1 | AES86571.1 | 87.6 | 129 | 9 | 1 | 1 | 122 | 1 | 129 | 1.00E-54 | 222.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60021.t1 | B7FK63 | 87.6 | 129 | 9 | 1 | 1 | 122 | 1 | 129 | 7.3e-55 | 222.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene60021 | 0.818853 | 1.58E-52 | -1.69E-46 |
| MS.gene050315 | MS.gene60021 | 0.815194 | 1.05E-51 | -1.69E-46 |
| MS.gene050406 | MS.gene60021 | 0.820741 | 5.84E-53 | -1.69E-46 |
| MS.gene050670 | MS.gene60021 | 0.818242 | 2.17E-52 | -1.69E-46 |
| MS.gene050812 | MS.gene60021 | 0.808003 | 3.83E-50 | -1.69E-46 |
| MS.gene051171 | MS.gene60021 | 0.827176 | 1.81E-54 | -1.69E-46 |
| MS.gene051208 | MS.gene60021 | 0.823825 | 1.12E-53 | -1.69E-46 |
| MS.gene051244 | MS.gene60021 | 0.804984 | 1.66E-49 | -1.69E-46 |
| MS.gene051525 | MS.gene60021 | 0.841468 | 4.67E-58 | -1.69E-46 |
| MS.gene051582 | MS.gene60021 | 0.870676 | 1.22E-66 | -1.69E-46 |
| MS.gene051666 | MS.gene60021 | 0.800769 | 1.23E-48 | -1.69E-46 |
| MS.gene051838 | MS.gene60021 | 0.805862 | 1.09E-49 | -1.69E-46 |
| MS.gene052011 | MS.gene60021 | -0.802426 | 5.64E-49 | -1.69E-46 |
| MS.gene052134 | MS.gene60021 | 0.829397 | 5.27E-55 | -1.69E-46 |
| MS.gene052135 | MS.gene60021 | 0.823765 | 1.16E-53 | -1.69E-46 |
| MS.gene052322 | MS.gene60021 | 0.800081 | 1.70E-48 | -1.69E-46 |
| MS.gene052481 | MS.gene60021 | 0.813771 | 2.16E-51 | -1.69E-46 |
| MS.gene052501 | MS.gene60021 | 0.850885 | 1.27E-60 | -1.69E-46 |
| MS.gene052503 | MS.gene60021 | 0.805642 | 1.21E-49 | -1.69E-46 |
| MS.gene052505 | MS.gene60021 | 0.839713 | 1.34E-57 | -1.69E-46 |
| MS.gene052589 | MS.gene60021 | 0.806524 | 7.89E-50 | -1.69E-46 |
| MS.gene052825 | MS.gene60021 | 0.803287 | 3.75E-49 | -1.69E-46 |
| MS.gene052982 | MS.gene60021 | 0.809618 | 1.73E-50 | -1.69E-46 |
| MS.gene053330 | MS.gene60021 | 0.891871 | 2.67E-74 | -1.69E-46 |
| MS.gene053971 | MS.gene60021 | 0.813435 | 2.56E-51 | -1.69E-46 |
| MS.gene054102 | MS.gene60021 | 0.849493 | 3.12E-60 | -1.69E-46 |
| MS.gene054110 | MS.gene60021 | 0.835446 | 1.67E-56 | -1.69E-46 |
| MS.gene055370 | MS.gene60021 | 0.862131 | 6.30E-64 | -1.69E-46 |
| MS.gene055539 | MS.gene60021 | 0.809776 | 1.60E-50 | -1.69E-46 |
| MS.gene05553 | MS.gene60021 | 0.832625 | 8.49E-56 | -1.69E-46 |
| MS.gene056633 | MS.gene60021 | 0.896673 | 2.94E-76 | -1.69E-46 |
| MS.gene056664 | MS.gene60021 | 0.829088 | 6.26E-55 | -1.69E-46 |
| MS.gene057048 | MS.gene60021 | 0.807536 | 4.82E-50 | -1.69E-46 |
| MS.gene057196 | MS.gene60021 | 0.842938 | 1.90E-58 | -1.69E-46 |
| MS.gene057310 | MS.gene60021 | 0.809237 | 2.09E-50 | -1.69E-46 |
| MS.gene057332 | MS.gene60021 | 0.812862 | 3.42E-51 | -1.69E-46 |
| MS.gene057658 | MS.gene60021 | 0.817337 | 3.47E-52 | -1.69E-46 |
| MS.gene058233 | MS.gene60021 | 0.846566 | 2.00E-59 | -1.69E-46 |
| MS.gene058276 | MS.gene60021 | 0.824989 | 5.99E-54 | -1.69E-46 |
| MS.gene058444 | MS.gene60021 | 0.837456 | 5.14E-57 | -1.69E-46 |
| MS.gene058544 | MS.gene60021 | 0.811871 | 5.64E-51 | -1.69E-46 |
| MS.gene058746 | MS.gene60021 | 0.890132 | 1.30E-73 | -1.69E-46 |
| MS.gene058838 | MS.gene60021 | 0.801681 | 8.03E-49 | -1.69E-46 |
| MS.gene059011 | MS.gene60021 | 0.804464 | 2.13E-49 | -1.69E-46 |
| MS.gene059141 | MS.gene60021 | 0.803988 | 2.68E-49 | -1.69E-46 |
| MS.gene05914 | MS.gene60021 | 0.805705 | 1.17E-49 | -1.69E-46 |
| MS.gene059512 | MS.gene60021 | 0.846138 | 2.62E-59 | -1.69E-46 |
| MS.gene060403 | MS.gene60021 | 0.823344 | 1.46E-53 | -1.69E-46 |
| MS.gene06092 | MS.gene60021 | 0.810951 | 8.93E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60021.t1 | MTR_4g010140 | 87.597 | 129 | 9 | 1 | 1 | 122 | 1 | 129 | 1.05e-71 | 213 |
| MS.gene60021.t1 | MTR_4g010140 | 87.597 | 129 | 9 | 1 | 1 | 122 | 1 | 129 | 2.48e-71 | 214 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60021.t1 | AT3G15840 | 53.600 | 125 | 44 | 4 | 2 | 122 | 4 | 118 | 1.86e-35 | 123 |
| MS.gene60021.t1 | AT3G15840 | 53.600 | 125 | 44 | 4 | 2 | 122 | 4 | 118 | 1.90e-35 | 123 |
| MS.gene60021.t1 | AT3G15840 | 60.674 | 89 | 30 | 1 | 34 | 122 | 15 | 98 | 1.03e-32 | 115 |
| MS.gene60021.t1 | AT3G15840 | 51.200 | 125 | 44 | 5 | 2 | 122 | 4 | 115 | 5.49e-31 | 111 |
| MS.gene60021.t1 | AT3G15840 | 67.742 | 62 | 20 | 0 | 61 | 122 | 3 | 64 | 2.87e-26 | 97.8 |
Find 22 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGCTCCTAGTGAAGAATT+TGG | 0.251751 | 4.3:+86510935 | MS.gene60021:CDS |
| TTGGGAAGGCTGCTGTATAC+TGG | 0.369413 | 4.3:+86510753 | MS.gene60021:CDS |
| ACCAAGATGTTACCCTTTGA+AGG | 0.390463 | 4.3:+86510151 | MS.gene60021:CDS |
| ATAGGTACAAGCTTCCTTCA+TGG | 0.408186 | 4.3:+86510717 | MS.gene60021:intron |
| TCCTTCAAAGGGTAACATCT+TGG | 0.415937 | 4.3:-86510152 | None:intergenic |
| AGTAGGAGCAACACCGTTAG+TGG | 0.435161 | 4.3:-86510780 | None:intergenic |
| TTCATATGTCTTTCCTTCAA+AGG | 0.436295 | 4.3:-86510164 | None:intergenic |
| TCATGGGCTATGTTTGAACT+TGG | 0.438144 | 4.3:+86510734 | MS.gene60021:CDS |
| TCATATGTCTTTCCTTCAAA+GGG | 0.449365 | 4.3:-86510163 | None:intergenic |
| TAGGTACAAGCTTCCTTCAT+GGG | 0.479108 | 4.3:+86510718 | MS.gene60021:intron |
| GGAGCAAGTTGAGCTGCAGC+TGG | 0.485205 | 4.3:-86510920 | None:intergenic |
| AATATCATACTTACTGAAGT+AGG | 0.495204 | 4.3:-86510797 | None:intergenic |
| GGCTATGTTTGAACTTGGGA+AGG | 0.522989 | 4.3:+86510739 | MS.gene60021:CDS |
| GCAATTCCAAATTCTTCACT+AGG | 0.523402 | 4.3:-86510941 | None:intergenic |
| CATGGGCTATGTTTGAACTT+GGG | 0.542521 | 4.3:+86510735 | MS.gene60021:CDS |
| TACTCTTGAATTTCTTCAGT+AGG | 0.550843 | 4.3:-86510230 | None:intergenic |
| GACAGAGAAGCAAGGTTGAG+TGG | 0.559534 | 4.3:-86509463 | None:intergenic |
| TGTAGTCAGTGCTGCTGCTG+CGG | 0.582718 | 4.3:+86510196 | MS.gene60021:CDS |
| AGTTCAAACATAGCCCATGA+AGG | 0.591842 | 4.3:-86510731 | None:intergenic |
| TGTTATGCTATGACTTCGAG+AGG | 0.628193 | 4.3:-86509490 | None:intergenic |
| GTATACTGGAAAACCACTAA+CGG | 0.651682 | 4.3:+86510767 | MS.gene60021:CDS |
| GAGGTTGTGACAGAGAAGCA+AGG | 0.664017 | 4.3:-86509471 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGTATTTATTTTTTAATAG+TGG | + | chr4.3:86509609-86509628 | MS.gene60021:intron | 10.0% |
| !!! | ACCTTTTTTTTTATAAATTT+TGG | + | chr4.3:86510867-86510886 | MS.gene60021:intron | 10.0% |
| !! | ATAAACATTAATAGGATATA+TGG | - | chr4.3:86510699-86510718 | None:intergenic | 15.0% |
| !! | GCCAAAATTTATAAAAAAAA+AGG | - | chr4.3:86510871-86510890 | None:intergenic | 15.0% |
| !! | GTTCATATATTTATTTATTG+TGG | - | chr4.3:86510594-86510613 | None:intergenic | 15.0% |
| !! | TAGAAATACAGAAAATATAA+AGG | + | chr4.3:86510482-86510501 | MS.gene60021:intron | 15.0% |
| !! | TATATCCTATTAATGTTTAT+AGG | + | chr4.3:86510699-86510718 | MS.gene60021:intron | 15.0% |
| !! | TATTATTGAGTTAATTCATT+AGG | + | chr4.3:86510816-86510835 | MS.gene60021:intron | 15.0% |
| !!! | ATTTATTTTTTAATAGTGGA+CGG | + | chr4.3:86509613-86509632 | MS.gene60021:intron | 15.0% |
| !!! | TTTTTTTATAAATTTTGGCA+GGG | + | chr4.3:86510872-86510891 | MS.gene60021:intron | 15.0% |
| !!! | TTTTTTTTATAAATTTTGGC+AGG | + | chr4.3:86510871-86510890 | MS.gene60021:intron | 15.0% |
| !! | AAAAGAAAAAGAGAGAAAAA+AGG | - | chr4.3:86509536-86509555 | None:intergenic | 20.0% |
| !! | TAATATTCTATCCTATAACA+TGG | - | chr4.3:86509814-86509833 | None:intergenic | 20.0% |
| !! | TTGTACCTATAAACATTAAT+AGG | - | chr4.3:86510707-86510726 | None:intergenic | 20.0% |
| !! | TTTGTTCATGTATTTGTTTA+TGG | - | chr4.3:86510548-86510567 | None:intergenic | 20.0% |
| !!! | AATATCATAGTCTAGTTTAA+TGG | - | chr4.3:86509855-86509874 | None:intergenic | 20.0% |
| !!! | TATTCAAACATTTTTTAACC+AGG | - | chr4.3:86509996-86510015 | None:intergenic | 20.0% |
| ! | AATATCATACTTACTGAAGT+AGG | - | chr4.3:86510800-86510819 | None:intergenic | 25.0% |
| ! | ACATTAATAGGATATATGGT+AGG | - | chr4.3:86510695-86510714 | None:intergenic | 25.0% |
| ! | ATCAATTCAATGATAGACTT+AGG | - | chr4.3:86509695-86509714 | None:intergenic | 25.0% |
| ! | CAATGGAGAAAAGTTAATAT+AGG | + | chr4.3:86510285-86510304 | MS.gene60021:intron | 25.0% |
| ! | CATTAATAGGATATATGGTA+GGG | - | chr4.3:86510694-86510713 | None:intergenic | 25.0% |
| ! | GAAAGGATAAATCATACTTA+AGG | + | chr4.3:86509925-86509944 | MS.gene60021:intron | 25.0% |
| ! | TATAAGAAGTTCCATGTTAT+AGG | + | chr4.3:86509800-86509819 | MS.gene60021:intron | 25.0% |
| !! | ATTTGATTATTTGTTGACTG+AGG | + | chr4.3:86509648-86509667 | MS.gene60021:intron | 25.0% |
| !!! | GAATTTGGAATTGCTTTTAA+TGG | + | chr4.3:86510950-86510969 | MS.gene60021:CDS | 25.0% |
| !!! | GTTTTAAAAACTGACTCAAA+TGG | - | chr4.3:86510457-86510476 | None:intergenic | 25.0% |
| !!! | TGATATTTTAAGCAAAGTCT+CGG | + | chr4.3:86509868-86509887 | MS.gene60021:intron | 25.0% |
| AATCCAACATTGGTATTAAG+TGG | - | chr4.3:86510263-86510282 | None:intergenic | 30.0% | |
| ACTGAAAACTAATCCTCATT+TGG | - | chr4.3:86509965-86509984 | None:intergenic | 30.0% | |
| AGGATAAATCATACTTAAGG+TGG | + | chr4.3:86509928-86509947 | MS.gene60021:intron | 30.0% | |
| ATCCAACATTGGTATTAAGT+GGG | - | chr4.3:86510262-86510281 | None:intergenic | 30.0% | |
| GAAAAAAAAACGTGGTTGAA+AGG | + | chr4.3:86509908-86509927 | MS.gene60021:intron | 30.0% | |
| GTTCATGTATTTGTTTATGG+TGG | - | chr4.3:86510545-86510564 | None:intergenic | 30.0% | |
| TACCAATGTTGGATTTACAA+TGG | + | chr4.3:86510268-86510287 | MS.gene60021:intron | 30.0% | |
| TACTCTTGAATTTCTTCAGT+AGG | - | chr4.3:86510233-86510252 | None:intergenic | 30.0% | |
| TCACTGTCTAATTATTTGAG+TGG | - | chr4.3:86510425-86510444 | None:intergenic | 30.0% | |
| TCATATGTCTTTCCTTCAAA+GGG | - | chr4.3:86510166-86510185 | None:intergenic | 30.0% | |
| TCGTAGATGAAAAAAAAACG+TGG | + | chr4.3:86509900-86509919 | MS.gene60021:intron | 30.0% | |
| TTCATATGTCTTTCCTTCAA+AGG | - | chr4.3:86510167-86510186 | None:intergenic | 30.0% | |
| ! | AGTTTTCAGTTAAGTAAACC+TGG | + | chr4.3:86509975-86509994 | MS.gene60021:intron | 30.0% |
| !!! | CAGTGTTTTGAAAACTGAAA+TGG | + | chr4.3:86510341-86510360 | MS.gene60021:intron | 30.0% |
| CATAGCATAACAAACACGTT+TGG | + | chr4.3:86509501-86509520 | MS.gene60021:intron | 35.0% | |
| CTCCATTGTAAATCCAACAT+TGG | - | chr4.3:86510273-86510292 | None:intergenic | 35.0% | |
| GCAATTCCAAATTCTTCACT+AGG | - | chr4.3:86510944-86510963 | None:intergenic | 35.0% | |
| GTATACTGGAAAACCACTAA+CGG | + | chr4.3:86510767-86510786 | MS.gene60021:CDS | 35.0% | |
| TTCCCACTTAATACCAATGT+TGG | + | chr4.3:86510257-86510276 | MS.gene60021:intron | 35.0% | |
| ACCAAGATGTTACCCTTTGA+AGG | + | chr4.3:86510151-86510170 | MS.gene60021:CDS | 40.0% | |
| AGTTCAAACATAGCCCATGA+AGG | - | chr4.3:86510734-86510753 | None:intergenic | 40.0% | |
| ATAGGTACAAGCTTCCTTCA+TGG | + | chr4.3:86510717-86510736 | MS.gene60021:intron | 40.0% | |
| CACATGACCAATTGCAAAGA+GGG | - | chr4.3:86509573-86509592 | None:intergenic | 40.0% | |
| CACTTCATAGTTTCTGATGC+AGG | + | chr4.3:86510104-86510123 | MS.gene60021:intron | 40.0% | |
| CTTGCTCCTAGTGAAGAATT+TGG | + | chr4.3:86510935-86510954 | MS.gene60021:CDS | 40.0% | |
| GTCATTGAACCATGACTTGA+AGG | - | chr4.3:86510384-86510403 | None:intergenic | 40.0% | |
| TAGGTACAAGCTTCCTTCAT+GGG | + | chr4.3:86510718-86510737 | MS.gene60021:intron | 40.0% | |
| TCCTTCAAAGGGTAACATCT+TGG | - | chr4.3:86510155-86510174 | None:intergenic | 40.0% | |
| TGTTATGCTATGACTTCGAG+AGG | - | chr4.3:86509493-86509512 | None:intergenic | 40.0% | |
| ! | CATGGGCTATGTTTGAACTT+GGG | + | chr4.3:86510735-86510754 | MS.gene60021:CDS | 40.0% |
| ! | GCAATTGGTCATGTGGATTA+TGG | + | chr4.3:86509578-86509597 | MS.gene60021:intron | 40.0% |
| ! | TCATGGGCTATGTTTGAACT+TGG | + | chr4.3:86510734-86510753 | MS.gene60021:CDS | 40.0% |
| CATACTTAAGGTGGTCAACG+AGG | + | chr4.3:86509937-86509956 | MS.gene60021:intron | 45.0% | |
| CCACATGACCAATTGCAAAG+AGG | - | chr4.3:86509574-86509593 | None:intergenic | 45.0% | |
| CCTCTTTGCAATTGGTCATG+TGG | + | chr4.3:86509571-86509590 | MS.gene60021:intron | 45.0% | |
| GAACCATGACTTGAAGGTCT+CGG | - | chr4.3:86510378-86510397 | None:intergenic | 45.0% | |
| GGCTATGTTTGAACTTGGGA+AGG | + | chr4.3:86510739-86510758 | MS.gene60021:CDS | 45.0% | |
| AGTAGGAGCAACACCGTTAG+TGG | - | chr4.3:86510783-86510802 | None:intergenic | 50.0% | |
| GACAGAGAAGCAAGGTTGAG+TGG | - | chr4.3:86509466-86509485 | None:intergenic | 50.0% | |
| GAGGTTGTGACAGAGAAGCA+AGG | - | chr4.3:86509474-86509493 | None:intergenic | 50.0% | |
| GAGTGCACCCTCTTTGCAAT+TGG | + | chr4.3:86509563-86509582 | MS.gene60021:intron | 50.0% | |
| GGTCAACGAGGTTCCAAATG+AGG | + | chr4.3:86509949-86509968 | MS.gene60021:intron | 50.0% | |
| TTGGGAAGGCTGCTGTATAC+TGG | + | chr4.3:86510753-86510772 | MS.gene60021:CDS | 50.0% | |
| TCGCCGAGACCTTCAAGTCA+TGG | + | chr4.3:86510372-86510391 | MS.gene60021:intron | 55.0% | |
| ! | TGTAGTCAGTGCTGCTGCTG+CGG | + | chr4.3:86510196-86510215 | MS.gene60021:CDS | 55.0% |
| ! | GGAGCAAGTTGAGCTGCAGC+TGG | - | chr4.3:86510923-86510942 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 86509429 | 86510994 | 86509429 | ID=MS.gene60021 |
| chr4.3 | mRNA | 86509429 | 86510994 | 86509429 | ID=MS.gene60021.t1;Parent=MS.gene60021 |
| chr4.3 | exon | 86509429 | 86509498 | 86509429 | ID=MS.gene60021.t1.exon1;Parent=MS.gene60021.t1 |
| chr4.3 | CDS | 86509429 | 86509498 | 86509429 | ID=cds.MS.gene60021.t1;Parent=MS.gene60021.t1 |
| chr4.3 | exon | 86510126 | 86510249 | 86510126 | ID=MS.gene60021.t1.exon2;Parent=MS.gene60021.t1 |
| chr4.3 | CDS | 86510126 | 86510249 | 86510126 | ID=cds.MS.gene60021.t1;Parent=MS.gene60021.t1 |
| chr4.3 | exon | 86510721 | 86510805 | 86510721 | ID=MS.gene60021.t1.exon3;Parent=MS.gene60021.t1 |
| chr4.3 | CDS | 86510721 | 86510805 | 86510721 | ID=cds.MS.gene60021.t1;Parent=MS.gene60021.t1 |
| chr4.3 | exon | 86510893 | 86510994 | 86510893 | ID=MS.gene60021.t1.exon4;Parent=MS.gene60021.t1 |
| chr4.3 | CDS | 86510893 | 86510994 | 86510893 | ID=cds.MS.gene60021.t1;Parent=MS.gene60021.t1 |
| Gene Sequence |
| Protein sequence |
