AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60091


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60091.t1 MTR_8g038440 92.248 258 19 1 1 258 1 257 1.90e-164 455
MS.gene60091.t1 MTR_3g017490 54.340 265 98 5 14 258 2 263 9.35e-87 259
MS.gene60091.t1 MTR_7g090230 67.742 155 48 1 102 256 273 425 1.88e-71 225
MS.gene60091.t1 MTR_1g051180 64.024 164 47 2 102 256 204 364 1.07e-68 216
MS.gene60091.t1 MTR_5g083110 46.766 201 92 6 60 255 48 238 2.81e-48 160
MS.gene60091.t1 MTR_3g465550 52.667 150 67 3 102 251 83 228 8.45e-48 158
MS.gene60091.t1 MTR_5g072400 48.077 156 76 3 100 255 103 253 1.12e-42 146
MS.gene60091.t1 MTR_7g106500 46.914 162 74 3 103 256 122 279 2.06e-42 146
MS.gene60091.t1 MTR_7g104720 45.402 174 84 3 89 256 237 405 1.35e-41 147
MS.gene60091.t1 MTR_7g010090 43.602 211 91 7 65 256 231 432 6.17e-39 140
MS.gene60091.t1 MTR_1g090400 45.098 153 79 2 103 255 221 368 1.13e-38 138
MS.gene60091.t1 MTR_3g071510 47.468 158 76 4 100 255 82 234 5.63e-38 133
MS.gene60091.t1 MTR_6g011640 37.900 219 112 7 54 257 189 398 1.62e-35 130
MS.gene60091.t1 MTR_8g106900 45.570 158 78 3 103 255 119 273 1.73e-33 122
MS.gene60091.t1 MTR_8g099665 40.764 157 89 2 103 255 211 367 1.09e-32 122
MS.gene60091.t1 MTR_3g030520 41.250 160 79 3 113 257 141 300 1.19e-26 105
MS.gene60091.t1 MTR_5g048870 43.373 83 47 0 106 188 68 150 7.21e-15 70.5
MS.gene60091.t1 MTR_1g095960 35.165 91 51 2 151 235 32 120 3.73e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60091.t1 AT5G13810 56.078 255 90 6 18 255 23 272 2.33e-87 261
MS.gene60091.t1 AT3G57070 61.146 157 60 1 101 257 262 417 6.71e-61 197
MS.gene60091.t1 AT2G41330 60.526 152 58 1 106 257 253 402 1.10e-56 186
MS.gene60091.t1 AT5G58530 50.000 162 73 3 100 255 106 265 2.17e-47 159
MS.gene60091.t1 AT3G28850 45.506 178 70 3 106 256 250 427 4.13e-39 141
MS.gene60091.t1 AT5G39865 47.619 168 69 5 106 256 224 389 7.66e-39 139
MS.gene60091.t1 AT5G06470 43.125 160 78 3 103 255 85 238 7.21e-37 130
MS.gene60091.t1 AT4G10630 40.933 193 98 5 78 255 142 333 8.44e-37 133
MS.gene60091.t1 AT1G32760 47.436 156 78 2 104 255 158 313 2.93e-36 131
MS.gene60091.t1 AT5G03870 38.767 227 127 4 35 255 163 383 8.28e-36 131
MS.gene60091.t1 AT1G64500 42.331 163 85 3 103 258 208 368 7.63e-34 125
MS.gene60091.t1 AT3G11773 42.953 149 78 4 108 255 7 149 6.87e-31 112
MS.gene60091.t1 AT5G01420 43.137 153 83 3 103 255 252 400 2.54e-30 117
MS.gene60091.t1 AT3G11773 42.982 114 59 3 142 255 92 199 6.24e-21 87.8

Find 54 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACGGTGCGTTCGAGGTTT+TGG 0.195818 8.4:-59719364 None:intergenic
TGGTGAATTGGGGAAGATTT+TGG 0.232721 8.4:+59719823 MS.gene60091:CDS
CTTTGTGAGGTTGGTGAATT+GGG 0.267297 8.4:+59719812 MS.gene60091:CDS
TGTTTGGATTGTAATGAAAA+TGG 0.281412 8.4:+59719971 MS.gene60091:CDS
GTGAGAGCTGTGGTGATGTT+AGG 0.304707 8.4:+59719879 MS.gene60091:CDS
TTTCAATGGATATTTGTTAT+AGG 0.316739 8.4:+59719684 MS.gene60091:CDS
CGTTCTTCATGAGATGGTTT+TGG 0.326719 8.4:-59719434 None:intergenic
GCTTTGTGAGGTTGGTGAAT+TGG 0.368885 8.4:+59719811 MS.gene60091:CDS
AGGGTTAATGAATAGGTGTT+TGG 0.389424 8.4:+59719955 MS.gene60091:CDS
AGGATGAAGGGTTAATGAAT+AGG 0.418071 8.4:+59719948 MS.gene60091:CDS
GAACGCACCGTTTCGCTTCG+CGG 0.422378 8.4:+59719374 MS.gene60091:CDS
TGCCGTGTGGGAATTGTTGT+GGG 0.428411 8.4:+59719906 MS.gene60091:CDS
GTACCGGGTAATTTAATAAC+CGG 0.434591 8.4:-59719542 None:intergenic
ACGAGGATTGTTATGCGGTT+AGG 0.449157 8.4:+59719618 MS.gene60091:CDS
AATGGATATTTGTTATAGGA+AGG 0.466420 8.4:+59719688 MS.gene60091:CDS
AACATTGCCGCGAAGCGAAA+CGG 0.484573 8.4:-59719381 None:intergenic
GAGGGGTTTCCGAAGACAAA+AGG 0.492372 8.4:+59719845 MS.gene60091:CDS
TTGCCGTGTGGGAATTGTTG+TGG 0.494413 8.4:+59719905 MS.gene60091:CDS
GAAGCGAAACGGTGCGTTCG+AGG 0.500394 8.4:-59719370 None:intergenic
CACAATTCGATCTTCTGTAC+CGG 0.501465 8.4:-59719558 None:intergenic
ATAAAGCAGCTTTGTGAGGT+TGG 0.517233 8.4:+59719803 MS.gene60091:CDS
AGTGATCGTTCTTCATGAGA+TGG 0.522242 8.4:-59719440 None:intergenic
GAAGAATGTGACGTTGCCGC+AGG 0.527795 8.4:+59719742 MS.gene60091:CDS
ACAACAATACGACGTCGTCT+AGG 0.530821 8.4:+59719273 MS.gene60091:CDS
CCTCAAGAGATACAACAATT+CGG 0.531573 8.4:-59720007 None:intergenic
CCGAATTGTTGTATCTCTTG+AGG 0.543890 8.4:+59720007 MS.gene60091:CDS
GTCTATTTCACGAGTTTACG+TGG 0.553100 8.4:+59719581 MS.gene60091:CDS
CCTCAACCGTTCTTTCACAT+CGG 0.555438 8.4:+59719304 MS.gene60091:CDS
TGACGAGCGAGATGTTTCAA+TGG 0.556242 8.4:+59719670 MS.gene60091:CDS
GCACCGGTTATTAAATTACC+CGG 0.560307 8.4:+59719539 MS.gene60091:CDS
GTGGGAATTGTTGTGGGAGT+AGG 0.568754 8.4:+59719912 MS.gene60091:CDS
CCGATGTGAAAGAACGGTTG+AGG 0.569163 8.4:-59719304 None:intergenic
GAATCATGTGGGTGGTGCTG+AGG 0.579201 8.4:+59719778 MS.gene60091:CDS
TTTATCCGAGGGAATCATGT+GGG 0.579756 8.4:+59719767 MS.gene60091:CDS
GAAGGAATTGATGAGTGTGA+TGG 0.580805 8.4:+59719706 MS.gene60091:CDS
CACCACCCACATGATTCCCT+CGG 0.588736 8.4:-59719772 None:intergenic
AGATACAACAATTCGGACAC+CGG 0.609638 8.4:-59720000 None:intergenic
GAATTGTTGTGGGAGTAGGA+AGG 0.615372 8.4:+59719916 MS.gene60091:CDS
GGGTTTCCGAAGACAAAAGG+TGG 0.618149 8.4:+59719848 MS.gene60091:CDS
ACAATTCGATCTTCTGTACC+GGG 0.625107 8.4:-59719557 None:intergenic
GAAATCGATTGATTATGCAC+CGG 0.626408 8.4:+59719523 MS.gene60091:CDS
GTAATGAAAATGGATTGATC+CGG 0.626470 8.4:+59719981 MS.gene60091:CDS
GGGTATGTTTGTGAGAGCTG+TGG 0.630131 8.4:+59719869 MS.gene60091:CDS
AAGGAATTGATGAGTGTGAT+GGG 0.632999 8.4:+59719707 MS.gene60091:CDS
ATCCGAGGGAATCATGTGGG+TGG 0.638690 8.4:+59719770 MS.gene60091:CDS
TGGAATTAGACGAACATACG+AGG 0.640954 8.4:+59719601 MS.gene60091:CDS
CTCCCACAACAATTCCCACA+CGG 0.650155 8.4:-59719908 None:intergenic
AAAATCAACAGAATCAACAT+CGG 0.654380 8.4:+59719481 MS.gene60091:CDS
TACGAACCGATGTGAAAGAA+CGG 0.655850 8.4:-59719310 None:intergenic
AACATACGAGGATTGTTATG+CGG 0.658386 8.4:+59719613 MS.gene60091:CDS
GGTGATAAAGCAGCTTTGTG+AGG 0.673728 8.4:+59719799 MS.gene60091:CDS
TTTGTGAGGTTGGTGAATTG+GGG 0.686084 8.4:+59719813 MS.gene60091:CDS
CGGTACAGAAGATCGAATTG+TGG 0.690796 8.4:+59719559 MS.gene60091:CDS
GGTTTCCGAAGACAAAAGGT+GGG 0.695423 8.4:+59719849 MS.gene60091:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCAATGGATATTTGTTAT+AGG + chr8.4:59719684-59719703 MS.gene60091:CDS 20.0%
! AAAATCAACAGAATCAACAT+CGG + chr8.4:59719481-59719500 MS.gene60091:CDS 25.0%
! TGTTTGGATTGTAATGAAAA+TGG + chr8.4:59719971-59719990 MS.gene60091:CDS 25.0%
!!! AATCGATTTCGATGATTTTA+AGG - chr8.4:59719513-59719532 None:intergenic 25.0%
!!! AATGGATATTTGTTATAGGA+AGG + chr8.4:59719688-59719707 MS.gene60091:CDS 25.0%
!!! ATGTTGATTCTGTTGATTTT+AGG - chr8.4:59719481-59719500 None:intergenic 25.0%
!!! TGTTGATTCTGTTGATTTTA+GGG - chr8.4:59719480-59719499 None:intergenic 25.0%
!! GATGATTTTGAGAGGTTTTA+GGG + chr8.4:59719640-59719659 MS.gene60091:CDS 30.0%
!! GTAATGAAAATGGATTGATC+CGG + chr8.4:59719981-59720000 MS.gene60091:CDS 30.0%
AACATACGAGGATTGTTATG+CGG + chr8.4:59719613-59719632 MS.gene60091:CDS 35.0%
AAGGAATTGATGAGTGTGAT+GGG + chr8.4:59719707-59719726 MS.gene60091:CDS 35.0%
AGGATGAAGGGTTAATGAAT+AGG + chr8.4:59719948-59719967 MS.gene60091:CDS 35.0%
AGGGTTAATGAATAGGTGTT+TGG + chr8.4:59719955-59719974 MS.gene60091:CDS 35.0%
GAAATCGATTGATTATGCAC+CGG + chr8.4:59719523-59719542 MS.gene60091:CDS 35.0%
GTACCGGGTAATTTAATAAC+CGG - chr8.4:59719545-59719564 None:intergenic 35.0%
! AAGGTTTTTGATGAGGATGA+AGG + chr8.4:59719935-59719954 MS.gene60091:CDS 35.0%
! AGGTTTTTGATGAGGATGAA+GGG + chr8.4:59719936-59719955 MS.gene60091:CDS 35.0%
!! GGATGATTTTGAGAGGTTTT+AGG + chr8.4:59719639-59719658 MS.gene60091:CDS 35.0%
!!! TTTGAGAGGTTTTAGGGTTT+GGG + chr8.4:59719646-59719665 MS.gene60091:CDS 35.0%
!!! TTTTGAGAGGTTTTAGGGTT+TGG + chr8.4:59719645-59719664 MS.gene60091:CDS 35.0%
ACAATTCGATCTTCTGTACC+GGG - chr8.4:59719560-59719579 None:intergenic 40.0%
AGATACAACAATTCGGACAC+CGG - chr8.4:59720003-59720022 None:intergenic 40.0%
AGTGATCGTTCTTCATGAGA+TGG - chr8.4:59719443-59719462 None:intergenic 40.0%
CACAATTCGATCTTCTGTAC+CGG - chr8.4:59719561-59719580 None:intergenic 40.0%
GAAGGAATTGATGAGTGTGA+TGG + chr8.4:59719706-59719725 MS.gene60091:CDS 40.0%
GTCTATTTCACGAGTTTACG+TGG + chr8.4:59719581-59719600 MS.gene60091:CDS 40.0%
TACGAACCGATGTGAAAGAA+CGG - chr8.4:59719313-59719332 None:intergenic 40.0%
TGGAATTAGACGAACATACG+AGG + chr8.4:59719601-59719620 MS.gene60091:CDS 40.0%
TTTATCCGAGGGAATCATGT+GGG + chr8.4:59719767-59719786 MS.gene60091:CDS 40.0%
! ACATACCCACCTTTTGTCTT+CGG - chr8.4:59719857-59719876 None:intergenic 40.0%
! ATAAAGCAGCTTTGTGAGGT+TGG + chr8.4:59719803-59719822 MS.gene60091:CDS 40.0%
! ATGTTAGGTTTTTGCCGTGT+GGG + chr8.4:59719894-59719913 MS.gene60091:CDS 40.0%
! CGTTCTTCATGAGATGGTTT+TGG - chr8.4:59719437-59719456 None:intergenic 40.0%
! GAATTGGGGAAGATTTTGGA+GGG + chr8.4:59719827-59719846 MS.gene60091:CDS 40.0%
! GAGTAGGAAGGTTTTTGATG+AGG + chr8.4:59719928-59719947 MS.gene60091:CDS 40.0%
! TGAATTGGGGAAGATTTTGG+AGG + chr8.4:59719826-59719845 MS.gene60091:CDS 40.0%
! TGGTGAATTGGGGAAGATTT+TGG + chr8.4:59719823-59719842 MS.gene60091:CDS 40.0%
! TTTTATCCGAGGGAATCATG+TGG + chr8.4:59719766-59719785 MS.gene60091:CDS 40.0%
!! AATTGGGGAAGATTTTGGAG+GGG + chr8.4:59719828-59719847 MS.gene60091:CDS 40.0%
!! CTTTGTGAGGTTGGTGAATT+GGG + chr8.4:59719812-59719831 MS.gene60091:CDS 40.0%
!! GCACCGGTTATTAAATTACC+CGG + chr8.4:59719539-59719558 MS.gene60091:CDS 40.0%
!! TTTGTGAGGTTGGTGAATTG+GGG + chr8.4:59719813-59719832 MS.gene60091:CDS 40.0%
!!! AGGTTTTGGGTTTTTTGTGG+CGG - chr8.4:59719353-59719372 None:intergenic 40.0%
!!! TCGAGGTTTTGGGTTTTTTG+TGG - chr8.4:59719356-59719375 None:intergenic 40.0%
!!! TCTTCATGAGATGGTTTTGG+TGG - chr8.4:59719434-59719453 None:intergenic 40.0%
ACAACAATACGACGTCGTCT+AGG + chr8.4:59719273-59719292 MS.gene60091:CDS 45.0%
ACGAGGATTGTTATGCGGTT+AGG + chr8.4:59719618-59719637 MS.gene60091:CDS 45.0%
CCTCAACCGTTCTTTCACAT+CGG + chr8.4:59719304-59719323 MS.gene60091:CDS 45.0%
CGGTACAGAAGATCGAATTG+TGG + chr8.4:59719559-59719578 MS.gene60091:CDS 45.0%
GAATTGTTGTGGGAGTAGGA+AGG + chr8.4:59719916-59719935 MS.gene60091:CDS 45.0%
GGTTTCCGAAGACAAAAGGT+GGG + chr8.4:59719849-59719868 MS.gene60091:CDS 45.0%
TGACGAGCGAGATGTTTCAA+TGG + chr8.4:59719670-59719689 MS.gene60091:CDS 45.0%
TTCCCTCGGATAAAAACCTG+CGG - chr8.4:59719761-59719780 None:intergenic 45.0%
! GATGTTAGGTTTTTGCCGTG+TGG + chr8.4:59719893-59719912 MS.gene60091:CDS 45.0%
! GCGGTTAGGATGATTTTGAG+AGG + chr8.4:59719632-59719651 MS.gene60091:CDS 45.0%
! GGTGATAAAGCAGCTTTGTG+AGG + chr8.4:59719799-59719818 MS.gene60091:CDS 45.0%
! TTTGTGGCGGTTTTTCATCG+TGG - chr8.4:59719340-59719359 None:intergenic 45.0%
!! GCTTTGTGAGGTTGGTGAAT+TGG + chr8.4:59719811-59719830 MS.gene60091:CDS 45.0%
AACATTGCCGCGAAGCGAAA+CGG - chr8.4:59719384-59719403 None:intergenic 50.0%
CCGATGTGAAAGAACGGTTG+AGG - chr8.4:59719307-59719326 None:intergenic 50.0%
CTCCCACAACAATTCCCACA+CGG - chr8.4:59719911-59719930 None:intergenic 50.0%
GAGGGGTTTCCGAAGACAAA+AGG + chr8.4:59719845-59719864 MS.gene60091:CDS 50.0%
GGGTATGTTTGTGAGAGCTG+TGG + chr8.4:59719869-59719888 MS.gene60091:CDS 50.0%
GGGTTTCCGAAGACAAAAGG+TGG + chr8.4:59719848-59719867 MS.gene60091:CDS 50.0%
GTGGGAATTGTTGTGGGAGT+AGG + chr8.4:59719912-59719931 MS.gene60091:CDS 50.0%
TGCCGTGTGGGAATTGTTGT+GGG + chr8.4:59719906-59719925 MS.gene60091:CDS 50.0%
TTGCCGTGTGGGAATTGTTG+TGG + chr8.4:59719905-59719924 MS.gene60091:CDS 50.0%
! AAACGGTGCGTTCGAGGTTT+TGG - chr8.4:59719367-59719386 None:intergenic 50.0%
! AACGGTGCGTTCGAGGTTTT+GGG - chr8.4:59719366-59719385 None:intergenic 50.0%
! GTGAGAGCTGTGGTGATGTT+AGG + chr8.4:59719879-59719898 MS.gene60091:CDS 50.0%
! TGCCGCAGGTTTTTATCCGA+GGG + chr8.4:59719756-59719775 MS.gene60091:CDS 50.0%
! TTGCCGCAGGTTTTTATCCG+AGG + chr8.4:59719755-59719774 MS.gene60091:CDS 50.0%
ATCCGAGGGAATCATGTGGG+TGG + chr8.4:59719770-59719789 MS.gene60091:CDS 55.0%
CACCACCCACATGATTCCCT+CGG - chr8.4:59719775-59719794 None:intergenic 55.0%
GAAGAATGTGACGTTGCCGC+AGG + chr8.4:59719742-59719761 MS.gene60091:CDS 55.0%
! GAATCATGTGGGTGGTGCTG+AGG + chr8.4:59719778-59719797 MS.gene60091:CDS 55.0%
GAACGCACCGTTTCGCTTCG+CGG + chr8.4:59719374-59719393 MS.gene60091:CDS 60.0%
GAAGCGAAACGGTGCGTTCG+AGG - chr8.4:59719373-59719392 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 59719251 59720027 59719251 ID=MS.gene60091
chr8.4 mRNA 59719251 59720027 59719251 ID=MS.gene60091.t1;Parent=MS.gene60091
chr8.4 exon 59719251 59720027 59719251 ID=MS.gene60091.t1.exon1;Parent=MS.gene60091.t1
chr8.4 CDS 59719251 59720027 59719251 ID=cds.MS.gene60091.t1;Parent=MS.gene60091.t1
Gene Sequence

>MS.gene60091

ATGGAAGAAATCAGAAACAACAACAACAATACGACGTCGTCTAGGTCTTCATTCCTCAACCGTTCTTTCACATCGGTTCGTACAAACCACGATGAAAAACCGCCACAAAAAACCCAAAACCTCGAACGCACCGTTTCGCTTCGCGGCAATGTTGTTAAAAAGCTCTGTAGTGTCTTCGAACCACCAAAACCATCTCATGAAGAACGATCACTTTTCAAATCAAAAACCCTAAAATCAACAGAATCAACATCGGATTCATCCTTAAAATCATCGAAATCGATTGATTATGCACCGGTTATTAAATTACCCGGTACAGAAGATCGAATTGTGGTCTATTTCACGAGTTTACGTGGAATTAGACGAACATACGAGGATTGTTATGCGGTTAGGATGATTTTGAGAGGTTTTAGGGTTTGGGTTGACGAGCGAGATGTTTCAATGGATATTTGTTATAGGAAGGAATTGATGAGTGTGATGGGTGAGAAGAGTATGAAGAATGTGACGTTGCCGCAGGTTTTTATCCGAGGGAATCATGTGGGTGGTGCTGAGGTGATAAAGCAGCTTTGTGAGGTTGGTGAATTGGGGAAGATTTTGGAGGGGTTTCCGAAGACAAAAGGTGGGTATGTTTGTGAGAGCTGTGGTGATGTTAGGTTTTTGCCGTGTGGGAATTGTTGTGGGAGTAGGAAGGTTTTTGATGAGGATGAAGGGTTAATGAATAGGTGTTTGGATTGTAATGAAAATGGATTGATCCGGTGTCCGAATTGTTGTATCTCTTGA

Protein sequence

>MS.gene60091.t1

MEEIRNNNNNTTSSRSSFLNRSFTSVRTNHDEKPPQKTQNLERTVSLRGNVVKKLCSVFEPPKPSHEERSLFKSKTLKSTESTSDSSLKSSKSIDYAPVIKLPGTEDRIVVYFTSLRGIRRTYEDCYAVRMILRGFRVWVDERDVSMDICYRKELMSVMGEKSMKNVTLPQVFIRGNHVGGAEVIKQLCEVGELGKILEGFPKTKGGYVCESCGDVRFLPCGNCCGSRKVFDEDEGLMNRCLDCNENGLIRCPNCCIS