Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056363.t1 | XP_013456272.1 | 99 | 584 | 6 | 0 | 1 | 584 | 1 | 584 | 0.00E+00 | 1129.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056363.t1 | Q84RR0 | 73.9 | 529 | 136 | 2 | 1 | 528 | 1 | 528 | 1.0e-238 | 827.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056363.t1 | A0A072UML4 | 99.0 | 584 | 6 | 0 | 1 | 584 | 1 | 584 | 0.0e+00 | 1129.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053272 | MS.gene056363 | -0.828487 | 8.75E-55 | -1.69E-46 |
| MS.gene056363 | MS.gene05686 | 0.851063 | 1.13E-60 | -1.69E-46 |
| MS.gene056363 | MS.gene057174 | 0.813463 | 2.53E-51 | -1.69E-46 |
| MS.gene056363 | MS.gene057208 | 0.811234 | 7.75E-51 | -1.69E-46 |
| MS.gene056363 | MS.gene058781 | 0.854991 | 8.51E-62 | -1.69E-46 |
| MS.gene056363 | MS.gene058783 | 0.825967 | 3.51E-54 | -1.69E-46 |
| MS.gene056363 | MS.gene05952 | 0.819035 | 1.43E-52 | -1.69E-46 |
| MS.gene056363 | MS.gene060933 | 0.811015 | 8.65E-51 | -1.69E-46 |
| MS.gene056363 | MS.gene060935 | 0.835024 | 2.13E-56 | -1.69E-46 |
| MS.gene056363 | MS.gene060937 | 0.836055 | 1.17E-56 | -1.69E-46 |
| MS.gene056363 | MS.gene061384 | 0.808258 | 3.38E-50 | -1.69E-46 |
| MS.gene056363 | MS.gene06643 | 0.805729 | 1.16E-49 | -1.69E-46 |
| MS.gene056363 | MS.gene067173 | 0.800769 | 1.23E-48 | -1.69E-46 |
| MS.gene056363 | MS.gene068941 | 0.828438 | 8.99E-55 | -1.69E-46 |
| MS.gene056363 | MS.gene069289 | 0.818027 | 2.43E-52 | -1.69E-46 |
| MS.gene056363 | MS.gene070301 | 0.825451 | 4.66E-54 | -1.69E-46 |
| MS.gene056363 | MS.gene070918 | 0.810945 | 8.95E-51 | -1.69E-46 |
| MS.gene056363 | MS.gene072041 | 0.840257 | 9.70E-58 | -1.69E-46 |
| MS.gene056363 | MS.gene074607 | 0.80988 | 1.52E-50 | -1.69E-46 |
| MS.gene056363 | MS.gene23932 | 0.810185 | 1.31E-50 | -1.69E-46 |
| MS.gene056363 | MS.gene24356 | 0.803702 | 3.07E-49 | -1.69E-46 |
| MS.gene056363 | MS.gene24851 | 0.817974 | 2.50E-52 | -1.69E-46 |
| MS.gene056363 | MS.gene24852 | 0.810774 | 9.75E-51 | -1.69E-46 |
| MS.gene056363 | MS.gene27997 | 0.817132 | 3.86E-52 | -1.69E-46 |
| MS.gene056363 | MS.gene32688 | 0.814317 | 1.64E-51 | -1.69E-46 |
| MS.gene056363 | MS.gene35324 | 0.838473 | 2.82E-57 | -1.69E-46 |
| MS.gene056363 | MS.gene35858 | 0.81397 | 1.95E-51 | -1.69E-46 |
| MS.gene056363 | MS.gene36100 | 0.806125 | 9.57E-50 | -1.69E-46 |
| MS.gene056363 | MS.gene36571 | 0.806624 | 7.51E-50 | -1.69E-46 |
| MS.gene056363 | MS.gene37848 | 0.802138 | 6.47E-49 | -1.69E-46 |
| MS.gene056363 | MS.gene38207 | 0.814161 | 1.77E-51 | -1.69E-46 |
| MS.gene056363 | MS.gene38759 | 0.869272 | 3.51E-66 | -1.69E-46 |
| MS.gene056363 | MS.gene39037 | 0.846394 | 2.23E-59 | -1.69E-46 |
| MS.gene056363 | MS.gene42653 | 0.808357 | 3.22E-50 | -1.69E-46 |
| MS.gene056363 | MS.gene43969 | 0.844624 | 6.74E-59 | -1.69E-46 |
| MS.gene056363 | MS.gene43972 | 0.839366 | 1.65E-57 | -1.69E-46 |
| MS.gene056363 | MS.gene47967 | 0.806228 | 9.11E-50 | -1.69E-46 |
| MS.gene056363 | MS.gene49320 | 0.811146 | 8.10E-51 | -1.69E-46 |
| MS.gene056363 | MS.gene51877 | 0.802088 | 6.62E-49 | -1.69E-46 |
| MS.gene056363 | MS.gene52609 | 0.838161 | 3.39E-57 | -1.69E-46 |
| MS.gene056363 | MS.gene52616 | 0.829939 | 3.89E-55 | -1.69E-46 |
| MS.gene056363 | MS.gene53391 | 0.803706 | 3.07E-49 | -1.69E-46 |
| MS.gene056363 | MS.gene55005 | 0.816068 | 6.69E-52 | -1.69E-46 |
| MS.gene056363 | MS.gene55699 | 0.80427 | 2.34E-49 | -1.69E-46 |
| MS.gene056363 | MS.gene55707 | 0.80372 | 3.05E-49 | -1.69E-46 |
| MS.gene056363 | MS.gene56130 | 0.800375 | 1.48E-48 | -1.69E-46 |
| MS.gene056363 | MS.gene59616 | 0.804147 | 2.48E-49 | -1.69E-46 |
| MS.gene056363 | MS.gene59697 | 0.810666 | 1.03E-50 | -1.69E-46 |
| MS.gene056363 | MS.gene60091 | 0.825913 | 3.62E-54 | -1.69E-46 |
| MS.gene056363 | MS.gene60092 | 0.806324 | 8.69E-50 | -1.69E-46 |
| MS.gene056363 | MS.gene61239 | 0.815497 | 8.97E-52 | -1.69E-46 |
| MS.gene056363 | MS.gene61993 | 0.811561 | 6.58E-51 | -1.69E-46 |
| MS.gene056363 | MS.gene63930 | 0.806414 | 8.32E-50 | -1.69E-46 |
| MS.gene056363 | MS.gene64772 | 0.803669 | 3.12E-49 | -1.69E-46 |
| MS.gene056363 | MS.gene66228 | 0.806173 | 9.35E-50 | -1.69E-46 |
| MS.gene056363 | MS.gene67662 | 0.810869 | 9.30E-51 | -1.69E-46 |
| MS.gene056363 | MS.gene68198 | 0.80856 | 2.92E-50 | -1.69E-46 |
| MS.gene056363 | MS.gene68543 | 0.814377 | 1.59E-51 | -1.69E-46 |
| MS.gene056363 | MS.gene69779 | 0.815422 | 9.32E-52 | -1.69E-46 |
| MS.gene056363 | MS.gene71236 | 0.824969 | 6.05E-54 | -1.69E-46 |
| MS.gene056363 | MS.gene73344 | 0.810689 | 1.02E-50 | -1.69E-46 |
| MS.gene056363 | MS.gene73644 | 0.833851 | 4.20E-56 | -1.69E-46 |
| MS.gene056363 | MS.gene74761 | -0.800017 | 1.76E-48 | -1.69E-46 |
| MS.gene056363 | MS.gene74819 | 0.825389 | 4.81E-54 | -1.69E-46 |
| MS.gene056363 | MS.gene74902 | 0.858019 | 1.10E-62 | -1.69E-46 |
| MS.gene056363 | MS.gene74904 | 0.85921 | 4.84E-63 | -1.69E-46 |
| MS.gene056363 | MS.gene76010 | 0.800609 | 1.33E-48 | -1.69E-46 |
| MS.gene056363 | MS.gene80201 | 0.80826 | 3.38E-50 | -1.69E-46 |
| MS.gene056363 | MS.gene80237 | 0.82157 | 3.76E-53 | -1.69E-46 |
| MS.gene056363 | MS.gene80729 | 0.806589 | 7.64E-50 | -1.69E-46 |
| MS.gene056363 | MS.gene84703 | 0.812326 | 4.49E-51 | -1.69E-46 |
| MS.gene056363 | MS.gene84734 | 0.801485 | 8.81E-49 | -1.69E-46 |
| MS.gene056363 | MS.gene88658 | 0.801221 | 9.97E-49 | -1.69E-46 |
| MS.gene056363 | MS.gene89154 | 0.822122 | 2.80E-53 | -1.69E-46 |
| MS.gene056363 | MS.gene92981 | 0.809774 | 1.60E-50 | -1.69E-46 |
| MS.gene056363 | MS.gene93123 | 0.814215 | 1.73E-51 | -1.69E-46 |
| MS.gene056363 | MS.gene94674 | 0.800182 | 1.62E-48 | -1.69E-46 |
| MS.gene056363 | MS.gene98636 | 0.827685 | 1.36E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056363.t1 | MTR_4g066650 | 98.973 | 584 | 6 | 0 | 1 | 584 | 1 | 584 | 0.0 | 1218 |
| MS.gene056363.t1 | MTR_2g098580 | 80.435 | 598 | 101 | 4 | 1 | 584 | 1 | 596 | 0.0 | 971 |
| MS.gene056363.t1 | MTR_3g065380 | 71.160 | 586 | 153 | 6 | 1 | 582 | 1 | 574 | 0.0 | 838 |
| MS.gene056363.t1 | MTR_3g065410 | 69.416 | 582 | 148 | 5 | 1 | 582 | 1 | 552 | 0.0 | 795 |
| MS.gene056363.t1 | MTR_5g097670 | 84.566 | 311 | 48 | 0 | 223 | 533 | 4 | 314 | 0.0 | 559 |
| MS.gene056363.t1 | MTR_4g075010 | 40.586 | 478 | 215 | 9 | 57 | 519 | 2 | 425 | 2.14e-116 | 358 |
| MS.gene056363.t1 | MTR_4g075030 | 39.785 | 372 | 183 | 11 | 38 | 395 | 10 | 354 | 1.72e-77 | 250 |
| MS.gene056363.t1 | MTR_4g068940 | 29.423 | 537 | 336 | 13 | 18 | 529 | 2 | 520 | 2.61e-64 | 221 |
| MS.gene056363.t1 | MTR_4g068940 | 29.423 | 537 | 336 | 13 | 18 | 529 | 2 | 520 | 4.98e-64 | 221 |
| MS.gene056363.t1 | MTR_4g096720 | 53.030 | 66 | 28 | 1 | 243 | 305 | 58 | 123 | 2.27e-16 | 77.4 |
| MS.gene056363.t1 | MTR_3g462590 | 23.932 | 234 | 167 | 7 | 102 | 329 | 62 | 290 | 4.37e-13 | 70.9 |
| MS.gene056363.t1 | MTR_3g462570 | 24.561 | 228 | 151 | 9 | 124 | 343 | 33 | 247 | 9.44e-12 | 68.2 |
| MS.gene056363.t1 | MTR_3g014030 | 27.632 | 228 | 151 | 10 | 113 | 334 | 1499 | 1718 | 5.19e-11 | 66.6 |
| MS.gene056363.t1 | MTR_1g105460 | 29.787 | 188 | 118 | 7 | 154 | 333 | 121 | 302 | 7.52e-11 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056363.t1 | AT2G31510 | 72.887 | 568 | 138 | 5 | 1 | 559 | 1 | 561 | 0.0 | 846 |
| MS.gene056363.t1 | AT1G65430 | 69.512 | 574 | 158 | 4 | 18 | 584 | 4 | 567 | 0.0 | 826 |
| MS.gene056363.t1 | AT2G31510 | 76.892 | 489 | 109 | 2 | 74 | 559 | 2 | 489 | 0.0 | 791 |
| MS.gene056363.t1 | AT2G31510 | 74.206 | 504 | 118 | 3 | 1 | 498 | 1 | 498 | 0.0 | 790 |
| MS.gene056363.t1 | AT1G05890 | 70.385 | 520 | 138 | 4 | 20 | 533 | 16 | 525 | 0.0 | 768 |
| MS.gene056363.t1 | AT1G05890 | 73.684 | 475 | 122 | 2 | 59 | 533 | 96 | 567 | 0.0 | 753 |
| MS.gene056363.t1 | AT2G31770 | 56.673 | 517 | 195 | 8 | 16 | 522 | 3 | 500 | 0.0 | 598 |
| MS.gene056363.t1 | AT2G31760 | 59.140 | 465 | 171 | 6 | 57 | 519 | 40 | 487 | 0.0 | 580 |
| MS.gene056363.t1 | AT2G31780 | 55.677 | 458 | 179 | 8 | 1 | 451 | 1 | 441 | 0.0 | 519 |
| MS.gene056363.t1 | AT1G05880 | 39.407 | 472 | 253 | 8 | 61 | 523 | 40 | 487 | 1.40e-107 | 332 |
| MS.gene056363.t1 | AT1G05880 | 39.195 | 472 | 247 | 10 | 61 | 523 | 40 | 480 | 4.58e-103 | 320 |
| MS.gene056363.t1 | AT1G05880 | 38.546 | 454 | 246 | 8 | 61 | 505 | 40 | 469 | 3.05e-97 | 305 |
| MS.gene056363.t1 | AT4G34370 | 30.309 | 551 | 331 | 18 | 19 | 535 | 2 | 533 | 7.99e-67 | 228 |
| MS.gene056363.t1 | AT2G16090 | 29.744 | 585 | 341 | 18 | 19 | 559 | 2 | 560 | 1.73e-63 | 219 |
| MS.gene056363.t1 | AT2G16090 | 29.188 | 579 | 346 | 16 | 19 | 559 | 2 | 554 | 2.44e-62 | 216 |
| MS.gene056363.t1 | AT2G16090 | 30.286 | 525 | 313 | 15 | 19 | 516 | 2 | 500 | 3.98e-62 | 214 |
| MS.gene056363.t1 | AT3G27710 | 29.412 | 476 | 294 | 14 | 62 | 516 | 45 | 499 | 1.25e-58 | 205 |
| MS.gene056363.t1 | AT2G16090 | 29.865 | 519 | 302 | 15 | 82 | 559 | 3 | 500 | 2.04e-58 | 204 |
| MS.gene056363.t1 | AT5G63750 | 29.681 | 502 | 295 | 16 | 57 | 519 | 6 | 488 | 3.66e-58 | 204 |
| MS.gene056363.t1 | AT2G16090 | 29.779 | 497 | 289 | 14 | 82 | 539 | 3 | 478 | 7.57e-57 | 199 |
| MS.gene056363.t1 | AT5G08730 | 27.484 | 473 | 307 | 10 | 58 | 523 | 6 | 449 | 7.77e-56 | 196 |
| MS.gene056363.t1 | AT5G63730 | 27.190 | 548 | 315 | 18 | 52 | 580 | 4 | 486 | 6.16e-50 | 181 |
| MS.gene056363.t1 | AT4G34370 | 33.069 | 378 | 208 | 14 | 187 | 535 | 5 | 366 | 6.03e-47 | 171 |
| MS.gene056363.t1 | AT3G27720 | 32.047 | 337 | 190 | 10 | 199 | 516 | 143 | 459 | 8.69e-43 | 160 |
| MS.gene056363.t1 | AT5G63760 | 27.711 | 498 | 270 | 21 | 113 | 580 | 2 | 439 | 3.07e-42 | 158 |
| MS.gene056363.t1 | AT5G63760 | 27.711 | 498 | 270 | 21 | 113 | 580 | 2 | 439 | 3.07e-42 | 158 |
| MS.gene056363.t1 | AT5G63760 | 27.711 | 498 | 270 | 21 | 113 | 580 | 2 | 439 | 3.07e-42 | 158 |
| MS.gene056363.t1 | AT5G63760 | 28.021 | 389 | 207 | 15 | 208 | 580 | 2 | 333 | 1.12e-33 | 132 |
| MS.gene056363.t1 | AT5G10370 | 27.092 | 251 | 169 | 9 | 87 | 330 | 1512 | 1755 | 1.09e-12 | 72.0 |
| MS.gene056363.t1 | AT4G01020 | 27.888 | 251 | 167 | 10 | 87 | 330 | 1509 | 1752 | 1.34e-11 | 68.6 |
| MS.gene056363.t1 | AT3G45580 | 29.469 | 207 | 124 | 10 | 136 | 329 | 172 | 369 | 4.07e-11 | 65.9 |
| MS.gene056363.t1 | AT2G26135 | 26.291 | 213 | 139 | 8 | 121 | 325 | 140 | 342 | 6.43e-11 | 65.1 |
Find 126 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAGCAAAATTCAAATTTAC+AGG | 0.232442 | 4.1:+34265252 | None:intergenic |
| ATCGACGTTGATTGCAAATC+TGG | 0.244897 | 4.1:+34261693 | None:intergenic |
| TTCGATTGGTCGCTTGCATT+TGG | 0.271459 | 4.1:+34265312 | None:intergenic |
| GATATCAGATTCCTTCAATA+TGG | 0.313478 | 4.1:+34267844 | None:intergenic |
| TATCTCACCTCGCTAGTATT+AGG | 0.330743 | 4.1:+34267163 | None:intergenic |
| TGGCCCACCGCTCATAATAA+TGG | 0.335297 | 4.1:+34264640 | None:intergenic |
| GGGAGCAGTAAGGGGAGAGC+TGG | 0.349500 | 4.1:-34261815 | MS.gene056363:CDS |
| CCCACTTGTGGTGCTGCTAT+TGG | 0.352732 | 4.1:-34266618 | MS.gene056363:CDS |
| TCGATTGGTCGCTTGCATTT+GGG | 0.365336 | 4.1:+34265313 | None:intergenic |
| TGTCATCTTCCTGTCTTACT+TGG | 0.369228 | 4.1:+34267822 | None:intergenic |
| TTCTACTTCGTCACCACAAT+TGG | 0.377659 | 4.1:-34267751 | MS.gene056363:intron |
| CCGAAAAGCGGTTGGCTTGT+TGG | 0.378590 | 4.1:-34267206 | MS.gene056363:CDS |
| TATGCACGATGCTCACGATA+TGG | 0.384878 | 4.1:-34268719 | MS.gene056363:CDS |
| GGGAGAGCTGGGAGAGGAAA+GGG | 0.387635 | 4.1:-34261803 | MS.gene056363:CDS |
| TATCCGATGTAGATGCTAAT+GGG | 0.391251 | 4.1:-34261877 | MS.gene056363:CDS |
| TTATATCGAAGACAATAAAA+AGG | 0.396653 | 4.1:-34266526 | MS.gene056363:intron |
| AGTCGTTGAATTCTTTAGAT+TGG | 0.402864 | 4.1:+34262068 | None:intergenic |
| ATCCATACTGTTTCTCATGC+TGG | 0.403926 | 4.1:-34266965 | MS.gene056363:CDS |
| CTATCCGATGTAGATGCTAA+TGG | 0.404396 | 4.1:-34261878 | MS.gene056363:CDS |
| GTATTAGGATGCTGGACAAC+TGG | 0.406004 | 4.1:+34267178 | None:intergenic |
| AACTATCCTCGCTCTGGGAT+TGG | 0.407143 | 4.1:-34267005 | MS.gene056363:CDS |
| TCCCAGCATGAGAAACAGTA+TGG | 0.418225 | 4.1:+34266963 | None:intergenic |
| TTTGAAAACTATCCTCGCTC+TGG | 0.436202 | 4.1:-34267011 | MS.gene056363:CDS |
| GGGGAGAGCTGGGAGAGGAA+AGG | 0.438486 | 4.1:-34261804 | MS.gene056363:CDS |
| GCAGCACCACAAGTGGGTTC+AGG | 0.439174 | 4.1:+34266624 | None:intergenic |
| TTAGTGAGGGCTTTAGAGAA+TGG | 0.439616 | 4.1:-34261902 | MS.gene056363:CDS |
| GGCCCACCGCTCATAATAAT+GGG | 0.441437 | 4.1:+34264641 | None:intergenic |
| CTGAAATGGACGTATGCATA+TGG | 0.442573 | 4.1:-34262313 | MS.gene056363:CDS |
| GGTCGCTTGCATTTGGGACA+TGG | 0.453953 | 4.1:+34265319 | None:intergenic |
| GTCACAGCTTAAGTTCATTA+CGG | 0.454130 | 4.1:-34262545 | MS.gene056363:CDS |
| TGTAGAAATCGTCATCTTCA+AGG | 0.456356 | 4.1:+34268692 | None:intergenic |
| CACTGGACGATGAGCCTCCT+CGG | 0.458483 | 4.1:+34265868 | None:intergenic |
| GGAGCAGTAAGGGGAGAGCT+GGG | 0.464600 | 4.1:-34261814 | MS.gene056363:CDS |
| GACTTCCGTACAAAATTAGC+TGG | 0.465010 | 4.1:-34262049 | MS.gene056363:CDS |
| AGAAATGGCGAAGAATTCAT+TGG | 0.469188 | 4.1:-34264673 | MS.gene056363:CDS |
| TACTGAAAGAAGAAGAGAAA+TGG | 0.475078 | 4.1:-34264688 | MS.gene056363:CDS |
| ACATCCAGGACCATCATTGA+TGG | 0.478158 | 4.1:+34266662 | None:intergenic |
| AGCAGCTAAGCAAGAGGGAG+TGG | 0.478756 | 4.1:-34264809 | MS.gene056363:intron |
| TCCATACTGTTTCTCATGCT+GGG | 0.479243 | 4.1:-34266964 | MS.gene056363:CDS |
| AGTGTAGGAGAGTGCTGAAA+TGG | 0.479640 | 4.1:-34262327 | MS.gene056363:CDS |
| ATAAGATCTACGAAGGTAAC+GGG | 0.483111 | 4.1:+34266545 | None:intergenic |
| CATTACGGAGGCCTGGCTGC+AGG | 0.489128 | 4.1:-34262530 | MS.gene056363:intron |
| TTAAGTTCATTACGGAGGCC+TGG | 0.489786 | 4.1:-34262537 | MS.gene056363:CDS |
| TCAAAGTGGTGTCCTGCCCC+TGG | 0.492940 | 4.1:-34266069 | MS.gene056363:CDS |
| CAGAGTCCGAAAACATGAAC+TGG | 0.496048 | 4.1:-34265805 | MS.gene056363:intron |
| GATACATACAGATTGGTTGC+TGG | 0.496836 | 4.1:+34264620 | None:intergenic |
| ATCTGATATCCAAGTAAGAC+AGG | 0.497385 | 4.1:-34267831 | MS.gene056363:CDS |
| ACCAATAGCAGCACCACAAG+TGG | 0.506994 | 4.1:+34266617 | None:intergenic |
| ACGTCCATCAATGATGGTCC+TGG | 0.513736 | 4.1:-34266666 | MS.gene056363:CDS |
| ATGAACTTAAGCTGTGACTC+AGG | 0.514145 | 4.1:+34262550 | None:intergenic |
| TACTGTTTCTCATGCTGGGA+AGG | 0.514636 | 4.1:-34266960 | MS.gene056363:intron |
| TATCTACATAGGTTATGCCT+TGG | 0.517708 | 4.1:-34264889 | MS.gene056363:intron |
| GAAATTGAATTTACCAATTG+TGG | 0.519364 | 4.1:+34267738 | None:intergenic |
| AAATGCTGCAGGGAGCAGTA+AGG | 0.522523 | 4.1:-34261825 | MS.gene056363:CDS |
| CCTCGCTAGTATTAGGATGC+TGG | 0.523850 | 4.1:+34267170 | None:intergenic |
| AGCATCGTGCATATCATCTT+CGG | 0.523950 | 4.1:+34268729 | None:intergenic |
| CGATTTCTACAGTGGTGATA+CGG | 0.524021 | 4.1:-34268680 | MS.gene056363:CDS |
| ATCAGTACGTCCATCAATGA+TGG | 0.526648 | 4.1:-34266672 | MS.gene056363:CDS |
| TTGAAAACTATCCTCGCTCT+GGG | 0.528875 | 4.1:-34267010 | MS.gene056363:CDS |
| ACCACAGTGCCACAGTCCAC+TGG | 0.533681 | 4.1:+34265851 | None:intergenic |
| CAAAATTAGCTGGATTGACT+AGG | 0.541759 | 4.1:-34262039 | MS.gene056363:intron |
| CAGCAATTCCTCAGTGCTGA+AGG | 0.548428 | 4.1:-34262091 | MS.gene056363:CDS |
| CAAATCATTTACCTGCAGCC+AGG | 0.552068 | 4.1:+34262519 | None:intergenic |
| TGAGCTCGTTGCTTTGCTGA+AGG | 0.552429 | 4.1:+34261850 | None:intergenic |
| ATAGGTTATGCCTTGGTGCA+TGG | 0.554336 | 4.1:-34264882 | MS.gene056363:intron |
| TACAGTGGTGATACGGAAGA+AGG | 0.559004 | 4.1:-34268673 | MS.gene056363:CDS |
| AATGCTGCAGGGAGCAGTAA+GGG | 0.560155 | 4.1:-34261824 | MS.gene056363:CDS |
| GAACTTCTGATGATAGTCAC+TGG | 0.562651 | 4.1:-34261763 | MS.gene056363:CDS |
| CAGTTCTTTGAGTATTTGCA+AGG | 0.563576 | 4.1:-34262256 | MS.gene056363:intron |
| CTACTTCGAGAATTTAGTGA+GGG | 0.566132 | 4.1:-34261915 | MS.gene056363:CDS |
| AGTAAGGGGAGAGCTGGGAG+AGG | 0.566304 | 4.1:-34261809 | MS.gene056363:CDS |
| TGCTGTGGCCAGCTTACATG+TGG | 0.566414 | 4.1:-34267041 | MS.gene056363:intron |
| ACTTAAGCTGTGACTCAGGT+TGG | 0.573116 | 4.1:+34262554 | None:intergenic |
| TCTTCCAGATAGTTGAGTGT+AGG | 0.573883 | 4.1:-34262342 | MS.gene056363:intron |
| GCATATTCACAACCAGGGGC+AGG | 0.573937 | 4.1:+34266057 | None:intergenic |
| CAGCAAAATTCAAATTTACA+GGG | 0.574826 | 4.1:+34265253 | None:intergenic |
| TCAGACCATGGTGAAAGAAC+TGG | 0.575256 | 4.1:-34264859 | MS.gene056363:CDS |
| GGGATTGGAATGGCTTCTTG+TGG | 0.575363 | 4.1:-34266990 | MS.gene056363:CDS |
| AGTGATTATGATGATGATGC+TGG | 0.577389 | 4.1:-34268634 | MS.gene056363:CDS |
| TCCTCGCTCTGGGATTGGAA+TGG | 0.579366 | 4.1:-34267000 | MS.gene056363:CDS |
| GTAGAAATCGTCATCTTCAA+GGG | 0.579796 | 4.1:+34268693 | None:intergenic |
| ACTGTGGCACTGTGGTAAAG+TGG | 0.587937 | 4.1:-34265844 | MS.gene056363:CDS |
| ACACCCATTATTATGAGCGG+TGG | 0.591402 | 4.1:-34264644 | MS.gene056363:CDS |
| TATAAGATCTACGAAGGTAA+CGG | 0.591928 | 4.1:+34266544 | None:intergenic |
| AGATGTCCTGAACCCACTTG+TGG | 0.595444 | 4.1:-34266630 | MS.gene056363:CDS |
| GAGCGAGTCCGAAAAGCGGT+TGG | 0.598853 | 4.1:-34267214 | MS.gene056363:CDS |
| CATCTCAACATCAAACATCC+AGG | 0.599803 | 4.1:+34266648 | None:intergenic |
| GTACTATTTACCTGAGCATG+AGG | 0.605750 | 4.1:-34262290 | MS.gene056363:CDS |
| ATGCTGCAGGGAGCAGTAAG+GGG | 0.605774 | 4.1:-34261823 | MS.gene056363:CDS |
| CACCCATTATTATGAGCGGT+GGG | 0.609091 | 4.1:-34264643 | MS.gene056363:CDS |
| TGAAATGGACGTATGCATAT+GGG | 0.610291 | 4.1:-34262312 | MS.gene056363:CDS |
| TTATGAAGCAGCTAAGCAAG+AGG | 0.623330 | 4.1:-34264815 | MS.gene056363:CDS |
| CGAGGAGGCTCATCGTCCAG+TGG | 0.625879 | 4.1:-34265867 | MS.gene056363:CDS |
| TGATACGGAAGAAGGTGCGA+TGG | 0.632626 | 4.1:-34268665 | MS.gene056363:CDS |
| GAAGATGACGATTTCTACAG+TGG | 0.633360 | 4.1:-34268688 | MS.gene056363:CDS |
| TATGAAGCAGCTAAGCAAGA+GGG | 0.635282 | 4.1:-34264814 | MS.gene056363:CDS |
| GATTGAGTCTCGTCGACCTG+AGG | 0.639074 | 4.1:-34268587 | MS.gene056363:intron |
| GCCATTCCAATCCCAGAGCG+AGG | 0.643551 | 4.1:+34266999 | None:intergenic |
| GACCATGGTGAAAGAACTGG+TGG | 0.643713 | 4.1:-34264856 | MS.gene056363:CDS |
| CCAATAGCAGCACCACAAGT+GGG | 0.645725 | 4.1:+34266618 | None:intergenic |
| CTTCGATATAAGATCTACGA+AGG | 0.648378 | 4.1:+34266538 | None:intergenic |
| GCTCATCGTCCAGTGGACTG+TGG | 0.650054 | 4.1:-34265860 | MS.gene056363:CDS |
| TACTATTTACCTGAGCATGA+GGG | 0.652156 | 4.1:-34262289 | MS.gene056363:CDS |
| ACTACTTCGAGAATTTAGTG+AGG | 0.655176 | 4.1:-34261916 | MS.gene056363:CDS |
| GTAACTGCATATTCACAACC+AGG | 0.655441 | 4.1:+34266051 | None:intergenic |
| AACAAAAGCTATACGAACAG+AGG | 0.655471 | 4.1:+34265992 | None:intergenic |
| AACTGCATATTCACAACCAG+GGG | 0.659172 | 4.1:+34266053 | None:intergenic |
| ATTGCAAATCTGGCATGTGG+TGG | 0.660392 | 4.1:+34261703 | None:intergenic |
| AACCACCAGTTCTTTCACCA+TGG | 0.660461 | 4.1:+34264854 | None:intergenic |
| CAAAATTCAAATTTACAGGG+TGG | 0.660925 | 4.1:+34265256 | None:intergenic |
| TCAGTAAAGTGCATGATGCA+TGG | 0.665829 | 4.1:-34267249 | MS.gene056363:CDS |
| TGAAGAGCGAGTCCGAAAAG+CGG | 0.666240 | 4.1:-34267218 | MS.gene056363:CDS |
| AGCCCCATTAGCATCTACAT+CGG | 0.667535 | 4.1:+34261874 | None:intergenic |
| TAACTGCATATTCACAACCA+GGG | 0.669788 | 4.1:+34266052 | None:intergenic |
| ATCCGATGTAGATGCTAATG+GGG | 0.671574 | 4.1:-34261876 | MS.gene056363:CDS |
| TAGATTGGCCTTCAGCACTG+AGG | 0.685503 | 4.1:+34262083 | None:intergenic |
| GATACACCCATTATTATGAG+CGG | 0.686220 | 4.1:-34264647 | MS.gene056363:CDS |
| TTGATTGCAAATCTGGCATG+TGG | 0.691443 | 4.1:+34261700 | None:intergenic |
| ACAGCTTAAGTTCATTACGG+AGG | 0.692076 | 4.1:-34262542 | MS.gene056363:CDS |
| CCAGCATCCTAATACTAGCG+AGG | 0.692364 | 4.1:-34267170 | MS.gene056363:intron |
| GAATCTACAGTGCACCGAGG+AGG | 0.695123 | 4.1:-34265882 | MS.gene056363:intron |
| CATGGTCTGACCATGCACCA+AGG | 0.699416 | 4.1:+34264872 | None:intergenic |
| CTTGGTGCATGGTCAGACCA+TGG | 0.701151 | 4.1:-34264871 | MS.gene056363:CDS |
| TCCAGTGGACTGTGGCACTG+TGG | 0.734400 | 4.1:-34265852 | MS.gene056363:CDS |
| GGCAGATTTGACATTCACAG+AGG | 0.749917 | 4.1:+34261724 | None:intergenic |
| ACTGAATCTACAGTGCACCG+AGG | 0.790165 | 4.1:-34265885 | MS.gene056363:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 34261691 | 34268758 | 34261691 | ID=MS.gene056363 |
| chr4.1 | mRNA | 34261691 | 34268758 | 34261691 | ID=MS.gene056363.t1;Parent=MS.gene056363 |
| chr4.1 | exon | 34268588 | 34268758 | 34268588 | ID=MS.gene056363.t1.exon1;Parent=MS.gene056363.t1 |
| chr4.1 | CDS | 34268588 | 34268758 | 34268588 | ID=cds.MS.gene056363.t1;Parent=MS.gene056363.t1 |
| chr4.1 | exon | 34267752 | 34267876 | 34267752 | ID=MS.gene056363.t1.exon2;Parent=MS.gene056363.t1 |
| chr4.1 | CDS | 34267752 | 34267876 | 34267752 | ID=cds.MS.gene056363.t1;Parent=MS.gene056363.t1 |
| chr4.1 | exon | 34267171 | 34267276 | 34267171 | ID=MS.gene056363.t1.exon3;Parent=MS.gene056363.t1 |
| chr4.1 | CDS | 34267171 | 34267276 | 34267171 | ID=cds.MS.gene056363.t1;Parent=MS.gene056363.t1 |
| chr4.1 | exon | 34266961 | 34267051 | 34266961 | ID=MS.gene056363.t1.exon4;Parent=MS.gene056363.t1 |
| chr4.1 | CDS | 34266961 | 34267051 | 34266961 | ID=cds.MS.gene056363.t1;Parent=MS.gene056363.t1 |
| chr4.1 | exon | 34266527 | 34266699 | 34266527 | ID=MS.gene056363.t1.exon5;Parent=MS.gene056363.t1 |
| chr4.1 | CDS | 34266527 | 34266699 | 34266527 | ID=cds.MS.gene056363.t1;Parent=MS.gene056363.t1 |
| chr4.1 | exon | 34265987 | 34266091 | 34265987 | ID=MS.gene056363.t1.exon6;Parent=MS.gene056363.t1 |
| chr4.1 | CDS | 34265987 | 34266091 | 34265987 | ID=cds.MS.gene056363.t1;Parent=MS.gene056363.t1 |
| chr4.1 | exon | 34265806 | 34265894 | 34265806 | ID=MS.gene056363.t1.exon7;Parent=MS.gene056363.t1 |
| chr4.1 | CDS | 34265806 | 34265894 | 34265806 | ID=cds.MS.gene056363.t1;Parent=MS.gene056363.t1 |
| chr4.1 | exon | 34265253 | 34265360 | 34265253 | ID=MS.gene056363.t1.exon8;Parent=MS.gene056363.t1 |
| chr4.1 | CDS | 34265253 | 34265360 | 34265253 | ID=cds.MS.gene056363.t1;Parent=MS.gene056363.t1 |
| chr4.1 | exon | 34264810 | 34264900 | 34264810 | ID=MS.gene056363.t1.exon9;Parent=MS.gene056363.t1 |
| chr4.1 | CDS | 34264810 | 34264900 | 34264810 | ID=cds.MS.gene056363.t1;Parent=MS.gene056363.t1 |
| chr4.1 | exon | 34264629 | 34264718 | 34264629 | ID=MS.gene056363.t1.exon10;Parent=MS.gene056363.t1 |
| chr4.1 | CDS | 34264629 | 34264718 | 34264629 | ID=cds.MS.gene056363.t1;Parent=MS.gene056363.t1 |
| chr4.1 | exon | 34262910 | 34262957 | 34262910 | ID=MS.gene056363.t1.exon11;Parent=MS.gene056363.t1 |
| chr4.1 | CDS | 34262910 | 34262957 | 34262910 | ID=cds.MS.gene056363.t1;Parent=MS.gene056363.t1 |
| chr4.1 | exon | 34262531 | 34262602 | 34262531 | ID=MS.gene056363.t1.exon12;Parent=MS.gene056363.t1 |
| chr4.1 | CDS | 34262531 | 34262602 | 34262531 | ID=cds.MS.gene056363.t1;Parent=MS.gene056363.t1 |
| chr4.1 | exon | 34262257 | 34262356 | 34262257 | ID=MS.gene056363.t1.exon13;Parent=MS.gene056363.t1 |
| chr4.1 | CDS | 34262257 | 34262356 | 34262257 | ID=cds.MS.gene056363.t1;Parent=MS.gene056363.t1 |
| chr4.1 | exon | 34262040 | 34262166 | 34262040 | ID=MS.gene056363.t1.exon14;Parent=MS.gene056363.t1 |
| chr4.1 | CDS | 34262040 | 34262166 | 34262040 | ID=cds.MS.gene056363.t1;Parent=MS.gene056363.t1 |
| chr4.1 | exon | 34261691 | 34261949 | 34261691 | ID=MS.gene056363.t1.exon15;Parent=MS.gene056363.t1 |
| chr4.1 | CDS | 34261691 | 34261949 | 34261691 | ID=cds.MS.gene056363.t1;Parent=MS.gene056363.t1 |
| Gene Sequence |
| Protein sequence |
