Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61142.t1 | XP_013445956.1 | 91.5 | 130 | 11 | 0 | 27 | 156 | 36 | 165 | 6.60E-64 | 253.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61142.t1 | Q39224 | 52.6 | 116 | 54 | 1 | 42 | 156 | 52 | 167 | 5.2e-33 | 142.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61142.t1 | A0A072TR49 | 91.5 | 130 | 11 | 0 | 27 | 156 | 41 | 170 | 4.8e-64 | 253.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene61142 | 0.821125 | 4.77E-53 | -1.69E-46 |
| MS.gene050647 | MS.gene61142 | 0.819601 | 1.07E-52 | -1.69E-46 |
| MS.gene051127 | MS.gene61142 | 0.812067 | 5.11E-51 | -1.69E-46 |
| MS.gene051131 | MS.gene61142 | 0.804943 | 1.69E-49 | -1.69E-46 |
| MS.gene051138 | MS.gene61142 | 0.804456 | 2.14E-49 | -1.69E-46 |
| MS.gene052211 | MS.gene61142 | 0.82919 | 5.91E-55 | -1.69E-46 |
| MS.gene05240 | MS.gene61142 | 0.831945 | 1.25E-55 | -1.69E-46 |
| MS.gene052606 | MS.gene61142 | 0.814565 | 1.44E-51 | -1.69E-46 |
| MS.gene053031 | MS.gene61142 | 0.810633 | 1.05E-50 | -1.69E-46 |
| MS.gene053033 | MS.gene61142 | 0.818339 | 2.06E-52 | -1.69E-46 |
| MS.gene053403 | MS.gene61142 | 0.813217 | 2.86E-51 | -1.69E-46 |
| MS.gene053406 | MS.gene61142 | 0.834475 | 2.93E-56 | -1.69E-46 |
| MS.gene053420 | MS.gene61142 | 0.815969 | 7.04E-52 | -1.69E-46 |
| MS.gene054146 | MS.gene61142 | 0.810644 | 1.04E-50 | -1.69E-46 |
| MS.gene054181 | MS.gene61142 | 0.801095 | 1.06E-48 | -1.69E-46 |
| MS.gene054742 | MS.gene61142 | 0.808959 | 2.40E-50 | -1.69E-46 |
| MS.gene05483 | MS.gene61142 | 0.807428 | 5.08E-50 | -1.69E-46 |
| MS.gene055124 | MS.gene61142 | 0.816145 | 6.43E-52 | -1.69E-46 |
| MS.gene055202 | MS.gene61142 | 0.807816 | 4.20E-50 | -1.69E-46 |
| MS.gene055240 | MS.gene61142 | 0.839157 | 1.87E-57 | -1.69E-46 |
| MS.gene056218 | MS.gene61142 | 0.842929 | 1.92E-58 | -1.69E-46 |
| MS.gene056590 | MS.gene61142 | 0.809384 | 1.94E-50 | -1.69E-46 |
| MS.gene056591 | MS.gene61142 | 0.81021 | 1.29E-50 | -1.69E-46 |
| MS.gene056748 | MS.gene61142 | 0.82879 | 7.39E-55 | -1.69E-46 |
| MS.gene057544 | MS.gene61142 | 0.821939 | 3.09E-53 | -1.69E-46 |
| MS.gene057550 | MS.gene61142 | 0.807735 | 4.37E-50 | -1.69E-46 |
| MS.gene057768 | MS.gene61142 | 0.807515 | 4.87E-50 | -1.69E-46 |
| MS.gene057820 | MS.gene61142 | 0.800204 | 1.61E-48 | -1.69E-46 |
| MS.gene058099 | MS.gene61142 | 0.805926 | 1.05E-49 | -1.69E-46 |
| MS.gene058171 | MS.gene61142 | 0.820078 | 8.29E-53 | -1.69E-46 |
| MS.gene058173 | MS.gene61142 | 0.806306 | 8.77E-50 | -1.69E-46 |
| MS.gene058175 | MS.gene61142 | 0.814 | 1.92E-51 | -1.69E-46 |
| MS.gene059037 | MS.gene61142 | 0.802681 | 5.00E-49 | -1.69E-46 |
| MS.gene059045 | MS.gene61142 | 0.813449 | 2.54E-51 | -1.69E-46 |
| MS.gene059953 | MS.gene61142 | 0.818731 | 1.68E-52 | -1.69E-46 |
| MS.gene059955 | MS.gene61142 | 0.800355 | 1.50E-48 | -1.69E-46 |
| MS.gene060087 | MS.gene61142 | 0.801295 | 9.63E-49 | -1.69E-46 |
| MS.gene060593 | MS.gene61142 | 0.810109 | 1.36E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 38 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGAGAATGGGGTTTCTTTC+AGG | 0.083963 | 5.1:-37380543 | MS.gene61142:intron |
| TCACGGAGTCAATCCTTTAT+TGG | 0.222974 | 5.1:-37379635 | MS.gene61142:CDS |
| CTAGTATTCCCCAACAATTC+AGG | 0.279797 | 5.1:-37379417 | None:intergenic |
| GGAATACTAGGAAATAAGTT+TGG | 0.339541 | 5.1:+37379430 | None:intergenic |
| ATCTCAGAAACCAGGTGATA+TGG | 0.344452 | 5.1:-37379548 | MS.gene61142:CDS |
| CTGAATTGTTGGGGAATACT+AGG | 0.351495 | 5.1:+37379418 | None:intergenic |
| CCAGGTGATATGGAAGGTTT+TGG | 0.352253 | 5.1:-37379538 | MS.gene61142:CDS |
| TCTCTTGGTGCCTTCTGTCC+AGG | 0.394890 | 5.1:-37380734 | MS.gene61142:CDS |
| GGTATCAGAGGATCACAAAT+TGG | 0.402340 | 5.1:-37379503 | MS.gene61142:CDS |
| TGATCATGCCTGCAGAGAAT+GGG | 0.418939 | 5.1:-37380555 | MS.gene61142:CDS |
| TTGATCATGCCTGCAGAGAA+TGG | 0.426303 | 5.1:-37380556 | MS.gene61142:CDS |
| GGGAAAGAACCTGAATTGTT+GGG | 0.431712 | 5.1:+37379408 | None:intergenic |
| TGGGTTGCTTTACTATCTCC+TGG | 0.435852 | 5.1:+37380716 | None:intergenic |
| AGGGAAAGAACCTGAATTGT+TGG | 0.436427 | 5.1:+37379407 | None:intergenic |
| AACATATCGTTCTGGAACTT+TGG | 0.450602 | 5.1:+37380690 | None:intergenic |
| TCGTTCTGGAACTTTGGTGA+TGG | 0.474377 | 5.1:+37380696 | None:intergenic |
| TAGATACAACTAGAGGGTCT+TGG | 0.489914 | 5.1:+37380660 | None:intergenic |
| TTTGGATTTCTCATGTATGA+TGG | 0.500988 | 5.1:+37379448 | None:intergenic |
| AAAGAAACCCCATTCTCTGC+AGG | 0.510694 | 5.1:+37380547 | None:intergenic |
| GAAGCTTGGAAGCTCTCTCT+TGG | 0.514547 | 5.1:-37380749 | None:intergenic |
| CGTTCTGGAACTTTGGTGAT+GGG | 0.517955 | 5.1:+37380697 | None:intergenic |
| AGAGGATCACAAATTGGAAT+GGG | 0.518486 | 5.1:-37379497 | MS.gene61142:CDS |
| CCAAAACCTTCCATATCACC+TGG | 0.521789 | 5.1:+37379538 | None:intergenic |
| CAGAGGATCACAAATTGGAA+TGG | 0.527609 | 5.1:-37379498 | MS.gene61142:CDS |
| AACTTATTCAAATCGATGAC+TGG | 0.529324 | 5.1:+37380614 | None:intergenic |
| CGGAGTCAATCCTTTATTGG+TGG | 0.547582 | 5.1:-37379632 | MS.gene61142:CDS |
| TTTACTATCTCCTGGACAGA+AGG | 0.556430 | 5.1:+37380724 | None:intergenic |
| TATTGGCAGCTGATTAATCA+CGG | 0.589335 | 5.1:-37379652 | MS.gene61142:intron |
| GATCATGCCTGCAGAGAATG+GGG | 0.590145 | 5.1:-37380554 | MS.gene61142:CDS |
| TCAACTGTTTGTGGTATCAG+AGG | 0.600452 | 5.1:-37379515 | MS.gene61142:CDS |
| CAGAAACCAGGTGATATGGA+AGG | 0.617232 | 5.1:-37379544 | MS.gene61142:CDS |
| AAATCGATGACTGGAACTTG+TGG | 0.617267 | 5.1:+37380623 | None:intergenic |
| TTTGTGCTAGATACAACTAG+AGG | 0.618176 | 5.1:+37380653 | None:intergenic |
| AAGTTGTTGTCAGAAGAAGA+TGG | 0.623206 | 5.1:-37380596 | MS.gene61142:CDS |
| GGAAAGAACCTGAATTGTTG+GGG | 0.633271 | 5.1:+37379409 | None:intergenic |
| TTGTGCTAGATACAACTAGA+GGG | 0.634845 | 5.1:+37380654 | None:intergenic |
| AGACTTCTTCAATCTACCAG+TGG | 0.636943 | 5.1:-37379587 | MS.gene61142:CDS |
| AAAATATTATCTCAGAAACC+AGG | 0.664490 | 5.1:-37379556 | MS.gene61142:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAAAAAAAAAAAAA+AGG | + | chr5.1:37379660-37379679 | None:intergenic | 0.0% |
| !! | AAAAAAAAAAAAAAAGGAGA+CGG | + | chr5.1:37379655-37379674 | None:intergenic | 15.0% |
| !! | AAAATAATCCATAAATATCT+CGG | + | chr5.1:37380091-37380110 | None:intergenic | 15.0% |
| !! | ATTTAGAAAATAGCAAAATA+AGG | - | chr5.1:37379772-37379791 | MS.gene61142:intron | 15.0% |
| !! | TAGAAATATATGTTAATAAG+TGG | - | chr5.1:37379846-37379865 | MS.gene61142:intron | 15.0% |
| !!! | AGTTCATGAAATATATATTT+TGG | - | chr5.1:37379979-37379998 | MS.gene61142:intron | 15.0% |
| !!! | ATGATATTTCTAATTATTAG+TGG | + | chr5.1:37379821-37379840 | None:intergenic | 15.0% |
| !!! | ATTAATGATCGAAATTTTTT+AGG | - | chr5.1:37379899-37379918 | MS.gene61142:intron | 15.0% |
| !!! | ATTATGGTTTTATTGTAATT+TGG | - | chr5.1:37380447-37380466 | MS.gene61142:intron | 15.0% |
| !!! | ATTGATATAGACTTATTTTT+TGG | - | chr5.1:37380388-37380407 | MS.gene61142:intron | 15.0% |
| !!! | TCATACTTCATTATTTTAAA+AGG | + | chr5.1:37379939-37379958 | None:intergenic | 15.0% |
| !!! | TGATATTTCTAATTATTAGT+GGG | + | chr5.1:37379820-37379839 | None:intergenic | 15.0% |
| !!! | TTAATGATCGAAATTTTTTA+GGG | - | chr5.1:37379900-37379919 | MS.gene61142:intron | 15.0% |
| !!! | TTATGGTTTTATTGTAATTT+GGG | - | chr5.1:37380448-37380467 | MS.gene61142:intron | 15.0% |
| !!! | TTTTAGTTGTATCATATTTA+TGG | - | chr5.1:37380002-37380021 | MS.gene61142:intron | 15.0% |
| !! | AAAATCATGGTTTATGTTAT+GGG | + | chr5.1:37380486-37380505 | None:intergenic | 20.0% |
| !! | TTTATAACCAAAATTTATCG+AGG | - | chr5.1:37380031-37380050 | MS.gene61142:intron | 20.0% |
| !!! | AACCATGATTTTGAAATTAT+TGG | - | chr5.1:37380494-37380513 | MS.gene61142:intron | 20.0% |
| !!! | AATTTGTTCTACTTTGTAAA+CGG | - | chr5.1:37380226-37380245 | MS.gene61142:intron | 20.0% |
| !!! | CCGAAATATAAATTTTAGAA+CGG | - | chr5.1:37380169-37380188 | MS.gene61142:intron | 20.0% |
| !!! | CCGTTCTAAAATTTATATTT+CGG | + | chr5.1:37380172-37380191 | None:intergenic | 20.0% |
| !!! | TTAAAATTTTCCACCAATAA+AGG | + | chr5.1:37380544-37380563 | None:intergenic | 20.0% |
| !!! | TTTTCACTTATTCAAAAGAA+CGG | - | chr5.1:37379687-37379706 | MS.gene61142:intron | 20.0% |
| ! | AAAATATTATCTCAGAAACC+AGG | - | chr5.1:37380607-37380626 | MS.gene61142:CDS | 25.0% |
| ! | CAAAATCATGGTTTATGTTA+TGG | + | chr5.1:37380487-37380506 | None:intergenic | 25.0% |
| ! | TCTCAATGGTTACATAATTA+TGG | - | chr5.1:37380431-37380450 | MS.gene61142:intron | 25.0% |
| ! | TGCCAATAATTTCAAAATCA+TGG | + | chr5.1:37380499-37380518 | None:intergenic | 25.0% |
| !!! | AATGGTTTTGAAAGAAAGAT+TGG | - | chr5.1:37379742-37379761 | MS.gene61142:intron | 25.0% |
| AACTTATTCAAATCGATGAC+TGG | + | chr5.1:37379552-37379571 | None:intergenic | 30.0% | |
| AAGCTAAACATTCAAAGACT+TGG | - | chr5.1:37380116-37380135 | MS.gene61142:intron | 30.0% | |
| AAGGACATTGTCTAAAAGAA+TGG | - | chr5.1:37379724-37379743 | MS.gene61142:intron | 30.0% | |
| AGAGACTTATGTAATCTCAA+TGG | - | chr5.1:37380417-37380436 | MS.gene61142:intron | 30.0% | |
| ATTTACAACAGTAGAAAGAG+AGG | - | chr5.1:37380196-37380215 | MS.gene61142:intron | 30.0% | |
| GGAATACTAGGAAATAAGTT+TGG | + | chr5.1:37380736-37380755 | None:intergenic | 30.0% | |
| GTTTGAGTCATACTTCATAA+GGG | - | chr5.1:37380248-37380267 | MS.gene61142:intron | 30.0% | |
| TTATAAATACCCATCGCTTT+CGG | - | chr5.1:37380322-37380341 | MS.gene61142:intron | 30.0% | |
| TTTGGATTTCTCATGTATGA+TGG | + | chr5.1:37380718-37380737 | None:intergenic | 30.0% | |
| ! | AATATTTTCTTCTCTTCCAC+TGG | + | chr5.1:37380595-37380614 | None:intergenic | 30.0% |
| AAGTTGTTGTCAGAAGAAGA+TGG | - | chr5.1:37379567-37379586 | MS.gene61142:CDS | 35.0% | |
| AATACGCTCCGAGATATTTA+TGG | - | chr5.1:37380080-37380099 | MS.gene61142:intron | 35.0% | |
| AGAGGATCACAAATTGGAAT+GGG | - | chr5.1:37380666-37380685 | MS.gene61142:CDS | 35.0% | |
| GGTTTGAGTCATACTTCATA+AGG | - | chr5.1:37380247-37380266 | MS.gene61142:intron | 35.0% | |
| TATTGGCAGCTGATTAATCA+CGG | - | chr5.1:37380511-37380530 | MS.gene61142:intron | 35.0% | |
| TTGTGCTAGATACAACTAGA+GGG | + | chr5.1:37379512-37379531 | None:intergenic | 35.0% | |
| TTTGATATTCCGAAAGCGAT+GGG | + | chr5.1:37380334-37380353 | None:intergenic | 35.0% | |
| TTTGTGCTAGATACAACTAG+AGG | + | chr5.1:37379513-37379532 | None:intergenic | 35.0% | |
| ! | GTTTTGTGAACATATCGTTC+TGG | + | chr5.1:37379484-37379503 | None:intergenic | 35.0% |
| ! | TTTTGATATTCCGAAAGCGA+TGG | + | chr5.1:37380335-37380354 | None:intergenic | 35.0% |
| !! | AACATATCGTTCTGGAACTT+TGG | + | chr5.1:37379476-37379495 | None:intergenic | 35.0% |
| AAATCGATGACTGGAACTTG+TGG | + | chr5.1:37379543-37379562 | None:intergenic | 40.0% | |
| AACGGTTGTGTGTTTGACAA+AGG | - | chr5.1:37379705-37379724 | MS.gene61142:intron | 40.0% | |
| AGACTTCTTCAATCTACCAG+TGG | - | chr5.1:37380576-37380595 | MS.gene61142:CDS | 40.0% | |
| ATCTCAGAAACCAGGTGATA+TGG | - | chr5.1:37380615-37380634 | MS.gene61142:CDS | 40.0% | |
| CAGAGGATCACAAATTGGAA+TGG | - | chr5.1:37380665-37380684 | MS.gene61142:CDS | 40.0% | |
| CTGAATTGTTGGGGAATACT+AGG | + | chr5.1:37380748-37380767 | None:intergenic | 40.0% | |
| GGTATCAGAGGATCACAAAT+TGG | - | chr5.1:37380660-37380679 | MS.gene61142:CDS | 40.0% | |
| TAGATACAACTAGAGGGTCT+TGG | + | chr5.1:37379506-37379525 | None:intergenic | 40.0% | |
| TCAACTGTTTGTGGTATCAG+AGG | - | chr5.1:37380648-37380667 | MS.gene61142:CDS | 40.0% | |
| TCACGGAGTCAATCCTTTAT+TGG | - | chr5.1:37380528-37380547 | MS.gene61142:intron | 40.0% | |
| TTTACTATCTCCTGGACAGA+AGG | + | chr5.1:37379442-37379461 | None:intergenic | 40.0% | |
| ! | TGCTCGTCCTCGATAAATTT+TGG | + | chr5.1:37380041-37380060 | None:intergenic | 40.0% |
| !! | AGGTTTTGGTCAACTGTTTG+TGG | - | chr5.1:37380639-37380658 | MS.gene61142:CDS | 40.0% |
| AAAGAAACCCCATTCTCTGC+AGG | + | chr5.1:37379619-37379638 | None:intergenic | 45.0% | |
| CAGAAACCAGGTGATATGGA+AGG | - | chr5.1:37380619-37380638 | MS.gene61142:CDS | 45.0% | |
| CAGAGAATGGGGTTTCTTTC+AGG | - | chr5.1:37379620-37379639 | MS.gene61142:CDS | 45.0% | |
| CCAAAACCTTCCATATCACC+TGG | + | chr5.1:37380628-37380647 | None:intergenic | 45.0% | |
| CGGAGTCAATCCTTTATTGG+TGG | - | chr5.1:37380531-37380550 | MS.gene61142:intron | 45.0% | |
| GGTGCGATGCTATCATTAGT+TGG | - | chr5.1:37380295-37380314 | MS.gene61142:intron | 45.0% | |
| TGATCATGCCTGCAGAGAAT+GGG | - | chr5.1:37379608-37379627 | MS.gene61142:CDS | 45.0% | |
| TTGATCATGCCTGCAGAGAA+TGG | - | chr5.1:37379607-37379626 | MS.gene61142:CDS | 45.0% | |
| ! | CCAGGTGATATGGAAGGTTT+TGG | - | chr5.1:37380625-37380644 | MS.gene61142:CDS | 45.0% |
| ! | TGGGTTGCTTTACTATCTCC+TGG | + | chr5.1:37379450-37379469 | None:intergenic | 45.0% |
| !! | CGTTCTGGAACTTTGGTGAT+GGG | + | chr5.1:37379469-37379488 | None:intergenic | 45.0% |
| !! | TCGTTCTGGAACTTTGGTGA+TGG | + | chr5.1:37379470-37379489 | None:intergenic | 45.0% |
| GAGTTCAACTCTCGTTGCTC+TGG | - | chr5.1:37380274-37380293 | MS.gene61142:intron | 50.0% | |
| GATCATGCCTGCAGAGAATG+GGG | - | chr5.1:37379609-37379628 | MS.gene61142:CDS | 50.0% | |
| !! | TCTCTTGGTGCCTTCTGTCC+AGG | - | chr5.1:37379429-37379448 | MS.gene61142:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 37379418 | 37380767 | 37379418 | ID=MS.gene61142 |
| chr5.1 | mRNA | 37379418 | 37380767 | 37379418 | ID=MS.gene61142.t1;Parent=MS.gene61142 |
| chr5.1 | exon | 37380544 | 37380767 | 37380544 | ID=MS.gene61142.t1.exon1;Parent=MS.gene61142.t1 |
| chr5.1 | CDS | 37380544 | 37380767 | 37380544 | ID=cds.MS.gene61142.t1;Parent=MS.gene61142.t1 |
| chr5.1 | exon | 37379418 | 37379665 | 37379418 | ID=MS.gene61142.t1.exon2;Parent=MS.gene61142.t1 |
| chr5.1 | CDS | 37379418 | 37379665 | 37379418 | ID=cds.MS.gene61142.t1;Parent=MS.gene61142.t1 |
| Gene Sequence |
| Protein sequence |
