Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64821.t1 | XP_003612257.2 | 94.2 | 154 | 8 | 1 | 1 | 154 | 1 | 153 | 3.30E-68 | 267.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64821.t1 | G7JYR8 | 94.2 | 154 | 8 | 1 | 1 | 154 | 1 | 153 | 2.4e-68 | 267.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049281 | MS.gene64821 | 0.835161 | 1.97E-56 | -1.69E-46 |
| MS.gene050170 | MS.gene64821 | -0.811939 | 5.45E-51 | -1.69E-46 |
| MS.gene050496 | MS.gene64821 | -0.865026 | 7.96E-65 | -1.69E-46 |
| MS.gene050504 | MS.gene64821 | -0.801714 | 7.90E-49 | -1.69E-46 |
| MS.gene050612 | MS.gene64821 | -0.812576 | 3.96E-51 | -1.69E-46 |
| MS.gene051044 | MS.gene64821 | 0.831456 | 1.65E-55 | -1.69E-46 |
| MS.gene051127 | MS.gene64821 | 0.803176 | 3.95E-49 | -1.69E-46 |
| MS.gene051364 | MS.gene64821 | 0.806574 | 7.70E-50 | -1.69E-46 |
| MS.gene051366 | MS.gene64821 | 0.813027 | 3.15E-51 | -1.69E-46 |
| MS.gene051651 | MS.gene64821 | 0.803006 | 4.28E-49 | -1.69E-46 |
| MS.gene052566 | MS.gene64821 | 0.803333 | 3.66E-49 | -1.69E-46 |
| MS.gene053420 | MS.gene64821 | 0.800451 | 1.43E-48 | -1.69E-46 |
| MS.gene054056 | MS.gene64821 | 0.80807 | 3.71E-50 | -1.69E-46 |
| MS.gene054954 | MS.gene64821 | -0.817519 | 3.16E-52 | -1.69E-46 |
| MS.gene054959 | MS.gene64821 | 0.816273 | 6.02E-52 | -1.69E-46 |
| MS.gene055152 | MS.gene64821 | 0.802281 | 6.04E-49 | -1.69E-46 |
| MS.gene055251 | MS.gene64821 | 0.816804 | 4.58E-52 | -1.69E-46 |
| MS.gene055776 | MS.gene64821 | -0.8164 | 5.64E-52 | -1.69E-46 |
| MS.gene05589 | MS.gene64821 | -0.829657 | 4.55E-55 | -1.69E-46 |
| MS.gene055942 | MS.gene64821 | 0.887441 | 1.42E-72 | -1.69E-46 |
| MS.gene056504 | MS.gene64821 | -0.837408 | 5.29E-57 | -1.69E-46 |
| MS.gene056761 | MS.gene64821 | 0.823602 | 1.27E-53 | -1.69E-46 |
| MS.gene058015 | MS.gene64821 | 0.811418 | 7.07E-51 | -1.69E-46 |
| MS.gene058268 | MS.gene64821 | 0.826684 | 2.37E-54 | -1.69E-46 |
| MS.gene058269 | MS.gene64821 | 0.804133 | 2.50E-49 | -1.69E-46 |
| MS.gene058334 | MS.gene64821 | 0.80905 | 2.29E-50 | -1.69E-46 |
| MS.gene058628 | MS.gene64821 | 0.82049 | 6.67E-53 | -1.69E-46 |
| MS.gene058629 | MS.gene64821 | 0.814739 | 1.32E-51 | -1.69E-46 |
| MS.gene058630 | MS.gene64821 | 0.812304 | 4.53E-51 | -1.69E-46 |
| MS.gene059022 | MS.gene64821 | -0.8252 | 5.34E-54 | -1.69E-46 |
| MS.gene059865 | MS.gene64821 | -0.825516 | 4.49E-54 | -1.69E-46 |
| MS.gene060076 | MS.gene64821 | 0.815633 | 8.36E-52 | -1.69E-46 |
| MS.gene060703 | MS.gene64821 | -0.81188 | 5.61E-51 | -1.69E-46 |
| MS.gene060778 | MS.gene64821 | -0.804089 | 2.55E-49 | -1.69E-46 |
| MS.gene06077 | MS.gene64821 | -0.804402 | 2.20E-49 | -1.69E-46 |
| MS.gene060982 | MS.gene64821 | -0.823743 | 1.18E-53 | -1.69E-46 |
| MS.gene061044 | MS.gene64821 | 0.817263 | 3.61E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64821.t1 | MTR_5g023030 | 95.455 | 154 | 6 | 1 | 1 | 154 | 1 | 153 | 2.34e-104 | 295 |
| MS.gene64821.t1 | MTR_5g023030 | 95.455 | 154 | 6 | 1 | 1 | 154 | 1 | 153 | 5.19e-104 | 295 |
| MS.gene64821.t1 | MTR_8g074760 | 54.658 | 161 | 60 | 7 | 1 | 154 | 1 | 155 | 5.87e-36 | 122 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTTCTGCTTTGGATTTCTA+AGG | 0.172324 | 5.1:+13889187 | MS.gene64821:CDS |
| TTAGGAGAAGTGGGTTCTTT+TGG | 0.237235 | 5.1:-13889068 | None:intergenic |
| TGCTTTCTTCTAGCTTCCTT+AGG | 0.309418 | 5.1:-13889023 | None:intergenic |
| TCACCCATGCACGAGACTTT+AGG | 0.317786 | 5.1:-13889086 | None:intergenic |
| AAATTGTTACATTCACCTTT+CGG | 0.403917 | 5.1:-13889342 | None:intergenic |
| AAGGACAAGAAGTTACATAA+AGG | 0.412042 | 5.1:+13889137 | MS.gene64821:CDS |
| GCTTTCTTCTAGCTTCCTTA+GGG | 0.417399 | 5.1:-13889022 | None:intergenic |
| AATGAAACCACATGTGTTGT+AGG | 0.443488 | 5.1:-13888996 | None:intergenic |
| AGGACAAGAAGTTACATAAA+GGG | 0.488699 | 5.1:+13889138 | MS.gene64821:CDS |
| TCTAAGGGAAGTTATGAAGC+AGG | 0.489058 | 5.1:+13889203 | MS.gene64821:CDS |
| TTCTCCTAAAGTCTCGTGCA+TGG | 0.496688 | 5.1:+13889082 | MS.gene64821:CDS |
| ATCGACAAGGACTAAGAGTT+GGG | 0.514111 | 5.1:-13888971 | None:intergenic |
| AAGAAATTTACCAGTGGGAG+AGG | 0.523630 | 5.1:+13889290 | MS.gene64821:CDS |
| GCACGAGACTTTAGGAGAAG+TGG | 0.524552 | 5.1:-13889078 | None:intergenic |
| TGTGGTTTCATTATTCCCTA+AGG | 0.534785 | 5.1:+13889007 | MS.gene64821:CDS |
| CAACCACCAGTGTCTTTAGC+GGG | 0.552346 | 5.1:+13888939 | MS.gene64821:CDS |
| AAGCTAGAAGAAAGCACAGA+AGG | 0.555212 | 5.1:+13889030 | MS.gene64821:CDS |
| TCATAGAATCCAACGTTGGA+GGG | 0.555841 | 5.1:-13889268 | None:intergenic |
| AGCTAGAAGAAAGCACAGAA+GGG | 0.558361 | 5.1:+13889031 | MS.gene64821:CDS |
| TTCATAGAATCCAACGTTGG+AGG | 0.559594 | 5.1:-13889269 | None:intergenic |
| GATCGACAAGGACTAAGAGT+TGG | 0.560227 | 5.1:-13888972 | None:intergenic |
| AGTGAATGTCCCTCCAACGT+TGG | 0.563858 | 5.1:+13889259 | MS.gene64821:CDS |
| CACGAGACTTTAGGAGAAGT+GGG | 0.567559 | 5.1:-13889077 | None:intergenic |
| TCTCCTAAAGTCTCGTGCAT+GGG | 0.572514 | 5.1:+13889083 | MS.gene64821:CDS |
| GTTCTGCTTTGGATTTCTAA+GGG | 0.577860 | 5.1:+13889188 | MS.gene64821:CDS |
| CTAAGGGAAGTTATGAAGCA+GGG | 0.583061 | 5.1:+13889204 | MS.gene64821:CDS |
| TGATATCCCGCTAAAGACAC+TGG | 0.590824 | 5.1:-13888945 | None:intergenic |
| ACAACCACCAGTGTCTTTAG+CGG | 0.593632 | 5.1:+13888938 | MS.gene64821:CDS |
| GAGACAAGGTGGGAAAGAGA+AGG | 0.596540 | 5.1:+13889226 | MS.gene64821:CDS |
| ACACAATGATCCTCTCCCAC+TGG | 0.608376 | 5.1:-13889300 | None:intergenic |
| AGTTATGAAGCAGGGAGACA+AGG | 0.608744 | 5.1:+13889212 | MS.gene64821:CDS |
| CTATGAAGAAATTTACCAGT+GGG | 0.611053 | 5.1:+13889285 | MS.gene64821:CDS |
| TTCTTCATAGAATCCAACGT+TGG | 0.612106 | 5.1:-13889272 | None:intergenic |
| TATGAAGCAGGGAGACAAGG+TGG | 0.615974 | 5.1:+13889215 | MS.gene64821:CDS |
| TTGATGCAACGCTATCCGAA+AGG | 0.628643 | 5.1:+13889327 | MS.gene64821:CDS |
| GCTAGAAGAAAGCACAGAAG+GGG | 0.641612 | 5.1:+13889032 | MS.gene64821:CDS |
| ATGAAGCAGGGAGACAAGGT+GGG | 0.641635 | 5.1:+13889216 | MS.gene64821:CDS |
| TCGATCACCTACAACACATG+TGG | 0.650680 | 5.1:+13888989 | MS.gene64821:CDS |
| TCTATGAAGAAATTTACCAG+TGG | 0.673132 | 5.1:+13889284 | MS.gene64821:CDS |
| TATCCCGCTAAAGACACTGG+TGG | 0.724982 | 5.1:-13888942 | None:intergenic |
| TGTGTTGTAGGTGATCGACA+AGG | 0.755971 | 5.1:-13888984 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGGACAAGAAGTTACATAA+AGG | + | chr5.1:13889137-13889156 | MS.gene64821:CDS | 30.0% | |
| AGGACAAGAAGTTACATAAA+GGG | + | chr5.1:13889138-13889157 | MS.gene64821:CDS | 30.0% | |
| CAAGAAGAAGAAGAAAAAGA+AGG | + | chr5.1:13889118-13889137 | MS.gene64821:CDS | 30.0% | |
| CTATGAAGAAATTTACCAGT+GGG | + | chr5.1:13889285-13889304 | MS.gene64821:CDS | 30.0% | |
| TCTATGAAGAAATTTACCAG+TGG | + | chr5.1:13889284-13889303 | MS.gene64821:CDS | 30.0% | |
| AATGAAACCACATGTGTTGT+AGG | - | chr5.1:13888999-13889018 | None:intergenic | 35.0% | |
| CAAACAAAAACGTTCTGCTT+TGG | + | chr5.1:13889177-13889196 | MS.gene64821:CDS | 35.0% | |
| TGTGGTTTCATTATTCCCTA+AGG | + | chr5.1:13889007-13889026 | MS.gene64821:CDS | 35.0% | |
| TTCTTCATAGAATCCAACGT+TGG | - | chr5.1:13889275-13889294 | None:intergenic | 35.0% | |
| ! | CAAAGCAGAACGTTTTTGTT+TGG | - | chr5.1:13889179-13889198 | None:intergenic | 35.0% |
| ! | GTTCTGCTTTGGATTTCTAA+GGG | + | chr5.1:13889188-13889207 | MS.gene64821:CDS | 35.0% |
| AAGAAATTTACCAGTGGGAG+AGG | + | chr5.1:13889290-13889309 | MS.gene64821:CDS | 40.0% | |
| ATCGACAAGGACTAAGAGTT+GGG | - | chr5.1:13888974-13888993 | None:intergenic | 40.0% | |
| CTAAGGGAAGTTATGAAGCA+GGG | + | chr5.1:13889204-13889223 | MS.gene64821:CDS | 40.0% | |
| TCATAGAATCCAACGTTGGA+GGG | - | chr5.1:13889271-13889290 | None:intergenic | 40.0% | |
| TCTAAGGGAAGTTATGAAGC+AGG | + | chr5.1:13889203-13889222 | MS.gene64821:CDS | 40.0% | |
| TTCATAGAATCCAACGTTGG+AGG | - | chr5.1:13889272-13889291 | None:intergenic | 40.0% | |
| ! | AAGCTAGAAGAAAGCACAGA+AGG | + | chr5.1:13889030-13889049 | MS.gene64821:CDS | 40.0% |
| ! | AGCTAGAAGAAAGCACAGAA+GGG | + | chr5.1:13889031-13889050 | MS.gene64821:CDS | 40.0% |
| ! | CGTTCTGCTTTGGATTTCTA+AGG | + | chr5.1:13889187-13889206 | MS.gene64821:CDS | 40.0% |
| !! | GCTTTCTTCTAGCTTCCTTA+GGG | - | chr5.1:13889025-13889044 | None:intergenic | 40.0% |
| !! | TGCTTTCTTCTAGCTTCCTT+AGG | - | chr5.1:13889026-13889045 | None:intergenic | 40.0% |
| !! | TTAGGAGAAGTGGGTTCTTT+TGG | - | chr5.1:13889071-13889090 | None:intergenic | 40.0% |
| ACAACCACCAGTGTCTTTAG+CGG | + | chr5.1:13888938-13888957 | MS.gene64821:CDS | 45.0% | |
| AGTTATGAAGCAGGGAGACA+AGG | + | chr5.1:13889212-13889231 | MS.gene64821:CDS | 45.0% | |
| GATCGACAAGGACTAAGAGT+TGG | - | chr5.1:13888975-13888994 | None:intergenic | 45.0% | |
| TCGATCACCTACAACACATG+TGG | + | chr5.1:13888989-13889008 | MS.gene64821:CDS | 45.0% | |
| TCTCCTAAAGTCTCGTGCAT+GGG | + | chr5.1:13889083-13889102 | MS.gene64821:CDS | 45.0% | |
| TGATATCCCGCTAAAGACAC+TGG | - | chr5.1:13888948-13888967 | None:intergenic | 45.0% | |
| TGTGTTGTAGGTGATCGACA+AGG | - | chr5.1:13888987-13889006 | None:intergenic | 45.0% | |
| TTCTCCTAAAGTCTCGTGCA+TGG | + | chr5.1:13889082-13889101 | MS.gene64821:CDS | 45.0% | |
| TTGATGCAACGCTATCCGAA+AGG | + | chr5.1:13889327-13889346 | MS.gene64821:CDS | 45.0% | |
| ! | CACGAGACTTTAGGAGAAGT+GGG | - | chr5.1:13889080-13889099 | None:intergenic | 45.0% |
| ! | GCTAGAAGAAAGCACAGAAG+GGG | + | chr5.1:13889032-13889051 | MS.gene64821:CDS | 45.0% |
| ACACAATGATCCTCTCCCAC+TGG | - | chr5.1:13889303-13889322 | None:intergenic | 50.0% | |
| AGTGAATGTCCCTCCAACGT+TGG | + | chr5.1:13889259-13889278 | MS.gene64821:CDS | 50.0% | |
| ATGAAGCAGGGAGACAAGGT+GGG | + | chr5.1:13889216-13889235 | MS.gene64821:CDS | 50.0% | |
| CAACCACCAGTGTCTTTAGC+GGG | + | chr5.1:13888939-13888958 | MS.gene64821:CDS | 50.0% | |
| GAGACAAGGTGGGAAAGAGA+AGG | + | chr5.1:13889226-13889245 | MS.gene64821:CDS | 50.0% | |
| TATCCCGCTAAAGACACTGG+TGG | - | chr5.1:13888945-13888964 | None:intergenic | 50.0% | |
| TATGAAGCAGGGAGACAAGG+TGG | + | chr5.1:13889215-13889234 | MS.gene64821:CDS | 50.0% | |
| TCACCCATGCACGAGACTTT+AGG | - | chr5.1:13889089-13889108 | None:intergenic | 50.0% | |
| ! | GCACGAGACTTTAGGAGAAG+TGG | - | chr5.1:13889081-13889100 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 13888888 | 13889352 | 13888888 | ID=MS.gene64821 |
| chr5.1 | mRNA | 13888888 | 13889352 | 13888888 | ID=MS.gene64821.t1;Parent=MS.gene64821 |
| chr5.1 | exon | 13888888 | 13889352 | 13888888 | ID=MS.gene64821.t1.exon1;Parent=MS.gene64821.t1 |
| chr5.1 | CDS | 13888888 | 13889352 | 13888888 | ID=cds.MS.gene64821.t1;Parent=MS.gene64821.t1 |
| Gene Sequence |
| Protein sequence |
