AlfalfaGEDB | MS.gene65279

M. sativa cultivar XinJiangDaYe / MS.gene65279

KEGG: K03946
GO: -
PFAM: PF05047.19

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65279.t1	XP_003594758.1	99	97	1	0	1	97	1	97	3.90E-46	193.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65279.t1	Q9FIJ2	74.2	97	25	0	1	97	1	97	1.9e-38	159.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65279.t1	G7IFY7	99.0	97	1	0	1	97	1	97	2.8e-46	193.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049283	MS.gene65279	-0.809973	1.45E-50	-1.69E-46
MS.gene049419	MS.gene65279	0.840905	6.56E-58	-1.69E-46
MS.gene049452	MS.gene65279	-0.801497	8.76E-49	-1.69E-46
MS.gene04991	MS.gene65279	0.808361	3.22E-50	-1.69E-46
MS.gene049983	MS.gene65279	-0.800428	1.45E-48	-1.69E-46
MS.gene050105	MS.gene65279	-0.870418	1.48E-66	-1.69E-46
MS.gene050654	MS.gene65279	-0.801749	7.77E-49	-1.69E-46
MS.gene050953	MS.gene65279	-0.802634	5.11E-49	-1.69E-46
MS.gene051122	MS.gene65279	-0.850684	1.45E-60	-1.69E-46
MS.gene051368	MS.gene65279	-0.822684	2.08E-53	-1.69E-46
MS.gene051848	MS.gene65279	0.805303	1.42E-49	-1.69E-46
MS.gene051924	MS.gene65279	0.822859	1.89E-53	-1.69E-46
MS.gene051926	MS.gene65279	0.819361	1.21E-52	-1.69E-46
MS.gene05216	MS.gene65279	-0.859598	3.70E-63	-1.69E-46
MS.gene052333	MS.gene65279	-0.830721	2.50E-55	-1.69E-46
MS.gene052586	MS.gene65279	-0.812427	4.26E-51	-1.69E-46
MS.gene052674	MS.gene65279	-0.866125	3.58E-65	-1.69E-46
MS.gene052675	MS.gene65279	-0.866731	2.30E-65	-1.69E-46
MS.gene052677	MS.gene65279	-0.870646	1.25E-66	-1.69E-46
MS.gene052965	MS.gene65279	0.802915	4.47E-49	-1.69E-46
MS.gene05415	MS.gene65279	-0.804139	2.49E-49	-1.69E-46
MS.gene054291	MS.gene65279	0.819286	1.26E-52	-1.69E-46
MS.gene054479	MS.gene65279	-0.860678	1.75E-63	-1.69E-46
MS.gene055225	MS.gene65279	-0.809505	1.83E-50	-1.69E-46
MS.gene055701	MS.gene65279	-0.842193	3.00E-58	-1.69E-46
MS.gene056045	MS.gene65279	-0.81272	3.68E-51	-1.69E-46
MS.gene056480	MS.gene65279	-0.833992	3.88E-56	-1.69E-46
MS.gene056779	MS.gene65279	-0.83314	6.33E-56	-1.69E-46
MS.gene056973	MS.gene65279	-0.805186	1.51E-49	-1.69E-46
MS.gene057156	MS.gene65279	0.839703	1.35E-57	-1.69E-46
MS.gene05761	MS.gene65279	-0.806093	9.73E-50	-1.69E-46
MS.gene058081	MS.gene65279	-0.820473	6.73E-53	-1.69E-46
MS.gene058223	MS.gene65279	0.997717	8.88E-248	-1.69E-46
MS.gene059161	MS.gene65279	-0.800185	1.62E-48	-1.69E-46
MS.gene060011	MS.gene65279	-0.810282	1.25E-50	-1.69E-46
MS.gene06052	MS.gene65279	-0.81565	8.29E-52	-1.69E-46
MS.gene060777	MS.gene65279	-0.836913	7.08E-57	-1.69E-46
MS.gene060802	MS.gene65279	-0.800814	1.21E-48	-1.69E-46
MS.gene060850	MS.gene65279	-0.817934	2.55E-52	-1.69E-46
MS.gene060851	MS.gene65279	-0.846515	2.07E-59	-1.69E-46
MS.gene060852	MS.gene65279	-0.844093	9.36E-59	-1.69E-46
MS.gene060932	MS.gene65279	-0.836576	8.63E-57	-1.69E-46
MS.gene06121	MS.gene65279	-0.832626	8.49E-56	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65279.t1	MTR_2g034290	98.969	97	1	0	1	97	1	97	1.09e-66	195
MS.gene65279.t1	MTR_8g012945	94.737	95	5	0	1	95	1	95	2.35e-55	167

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65279.t1	AT5G47890	74.227	97	25	0	1	97	1	97	3.40e-53	161

Find 23 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGATCAATATGGGCAGTTT+TGG	0.205012	2.2:-54657077	None:intergenic
ACTGCATTCACGGATCAATA+TGG	0.266079	2.2:-54657087	None:intergenic
TACCATATCTTGCCCACAAT+TGG	0.333397	2.2:-54657121	None:intergenic
CCCCAATTGTGGGCAAGATA+TGG	0.341515	2.2:+54657119	MS.gene65279:CDS
AACCTTGCAGATGAGCTTGC+AGG	0.383491	2.2:-54656887	None:intergenic
ACCATATCTTGCCCACAATT+GGG	0.391447	2.2:-54657120	None:intergenic
TTTGAAGGCACTCGAAGATC+TGG	0.433173	2.2:+54657987	MS.gene65279:CDS
TAAGCAGTAATATCAAGCTT+TGG	0.441770	2.2:-54658025	None:intergenic
GTTCAACTCCACTGCATTCA+CGG	0.460928	2.2:-54657097	None:intergenic
CTAGATTTGGGTGTTGAGAA+AGG	0.466386	2.2:+54657931	MS.gene65279:intron
CTGCATTCACGGATCAATAT+GGG	0.474327	2.2:-54657086	None:intergenic
TGTTGAGAAAGGCATCAAAC+TGG	0.506700	2.2:+54657942	MS.gene65279:CDS
TGGAGTTGAACCCCAATTGT+GGG	0.534156	2.2:+54657109	MS.gene65279:CDS
ATGAGCTTGCAGGTGATGAC+TGG	0.535904	2.2:-54656877	None:intergenic
TGCTTTGGAAAGTAATGGCA+TGG	0.538620	2.2:+54656807	None:intergenic
CCATATCTTGCCCACAATTG+GGG	0.545870	2.2:-54657119	None:intergenic
CTCGAAGATCTGGTAAAAGC+AGG	0.549599	2.2:+54657997	MS.gene65279:CDS
GTGGAGTTGAACCCCAATTG+TGG	0.601433	2.2:+54657108	MS.gene65279:CDS
CACCTGCAAGCTCATCTGCA+AGG	0.602497	2.2:+54656885	MS.gene65279:CDS
GAGAAAGGCATCAAACTGGA+AGG	0.617748	2.2:+54657946	MS.gene65279:CDS
TCGAGTGCCTTCAAAATCTG+TGG	0.622764	2.2:-54657979	None:intergenic
ATATTGATCCGTGAATGCAG+TGG	0.663327	2.2:+54657089	MS.gene65279:CDS
TGGAAAGTAATGGCATGGAG+AGG	0.677012	2.2:+54656812	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGTATTAATGATTAAATTAT+AGG	-	chr2.2:54657913-54657932	None:intergenic	10.0%
!!	TAAATTCATTTCAATGTTAA+AGG	+	chr2.2:54657347-54657366	MS.gene65279:intron	15.0%
!!	TAACATTGAAATGAATTTAA+GGG	-	chr2.2:54657346-54657365	None:intergenic	15.0%
!!	TAATCATTAATACTAGATTT+GGG	+	chr2.2:54657919-54657938	MS.gene65279:intron	15.0%
!!	TTAACATTGAAATGAATTTA+AGG	-	chr2.2:54657347-54657366	None:intergenic	15.0%
!!	TTAATCATTAATACTAGATT+TGG	+	chr2.2:54657918-54657937	MS.gene65279:intron	15.0%
!!!	AATTAATGCTATGAAATTTT+AGG	-	chr2.2:54656940-54656959	None:intergenic	15.0%
!!!	TTTTGTTGAAAAGAATTATA+AGG	+	chr2.2:54657037-54657056	MS.gene65279:CDS	15.0%
!!	AAAAAGAAAGAGATTTAAGA+TGG	-	chr2.2:54657151-54657170	None:intergenic	20.0%
!!	ATACAAAAATTTACAGTATG+TGG	-	chr2.2:54657790-54657809	None:intergenic	20.0%
!!	ATTATACACAACTTCTAATT+GGG	-	chr2.2:54657691-54657710	None:intergenic	20.0%
!!	GTATTAATGAATGGAAAAAT+TGG	+	chr2.2:54657617-54657636	MS.gene65279:intron	20.0%
!!	TGATTAAATTATAGGATGTT+AGG	-	chr2.2:54657905-54657924	None:intergenic	20.0%
!!!	AAAAGAAAGAGATTTAAGAT+GGG	-	chr2.2:54657150-54657169	None:intergenic	20.0%
!!!	AAATTTTAGGTTCAAAGATA+GGG	-	chr2.2:54656927-54656946	None:intergenic	20.0%
!!!	TAATTTTCTTTATGCATTCA+AGG	-	chr2.2:54657765-54657784	None:intergenic	20.0%
!!!	TACTATTTTTTCTTATCTCA+GGG	+	chr2.2:54657013-54657032	MS.gene65279:intron	20.0%
!!!	TTACTATTTTTTCTTATCTC+AGG	+	chr2.2:54657012-54657031	MS.gene65279:intron	20.0%
!	AGTTGTGTATAATGTCAATT+CGG	+	chr2.2:54657698-54657717	MS.gene65279:intron	25.0%
!	ATCCTTCAAGTATTAATGAA+TGG	+	chr2.2:54657608-54657627	MS.gene65279:intron	25.0%
!	CATTATACACAACTTCTAAT+TGG	-	chr2.2:54657692-54657711	None:intergenic	25.0%
!	TATAATATTCTCATCTCTGA+AGG	-	chr2.2:54657493-54657512	None:intergenic	25.0%
!	TTCCATTCATTAATACTTGA+AGG	-	chr2.2:54657613-54657632	None:intergenic	25.0%
!!	AAAAGAATGCTTTGAGTTAT+TGG	-	chr2.2:54657373-54657392	None:intergenic	25.0%
!!!	AATAACTCAAAGCATTCTTT+TGG	+	chr2.2:54657372-54657391	MS.gene65279:intron	25.0%
!!!	AATTTTAGGTTCAAAGATAG+GGG	-	chr2.2:54656926-54656945	None:intergenic	25.0%
!!!	GAAATTTTAGGTTCAAAGAT+AGG	-	chr2.2:54656928-54656947	None:intergenic	25.0%
!!!	GAGATAAATTTGTTAGTATC+TGG	+	chr2.2:54657413-54657432	MS.gene65279:intron	25.0%
!!!	TAAGATTATTTTGCGTATTG+TGG	+	chr2.2:54657735-54657754	MS.gene65279:intron	25.0%
	ACAAAATAATGCAGATGAGA+AGG	-	chr2.2:54657863-54657882	None:intergenic	30.0%
	ATGAATGGAAAAATTGGAGT+TGG	+	chr2.2:54657623-54657642	MS.gene65279:intron	30.0%
	ATTTGTTTGTATGAATCGCA+AGG	+	chr2.2:54657577-54657596	MS.gene65279:intron	30.0%
	GTATACTTACTCTTCAAAGA+TGG	+	chr2.2:54657806-54657825	MS.gene65279:intron	30.0%
	TTAAGGAACTAAGGAGAATA+TGG	-	chr2.2:54657211-54657230	None:intergenic	30.0%
!	CATCATGTATAGTGTTCTTT+TGG	+	chr2.2:54657440-54657459	MS.gene65279:intron	30.0%
!	GTTTTTAACCAACCGATAAA+GGG	-	chr2.2:54657661-54657680	None:intergenic	30.0%
!	TTTTTAACCAACCGATAAAG+GGG	-	chr2.2:54657660-54657679	None:intergenic	30.0%
!!	ATTATTTTGCGTATTGTGGA+TGG	+	chr2.2:54657739-54657758	MS.gene65279:intron	30.0%
!!	TGTTTTTAACCAACCGATAA+AGG	-	chr2.2:54657662-54657681	None:intergenic	30.0%
!!	TTGGTGGTTAATTTGTTAGA+GGG	+	chr2.2:54657391-54657410	MS.gene65279:intron	30.0%
!!	TTTGGTGGTTAATTTGTTAG+AGG	+	chr2.2:54657390-54657409	MS.gene65279:intron	30.0%
	AAATCCCAGTTAAGGAACTA+AGG	-	chr2.2:54657220-54657239	None:intergenic	35.0%
	AAGAACTTTCACCCCTTTAT+CGG	+	chr2.2:54657646-54657665	MS.gene65279:intron	35.0%
	ATTCTCCTTAGTTCCTTAAC+TGG	+	chr2.2:54657212-54657231	MS.gene65279:intron	35.0%
	TTCTCCTTAGTTCCTTAACT+GGG	+	chr2.2:54657213-54657232	MS.gene65279:intron	35.0%
!!	AACTCAAAGCATTCTTTTGG+TGG	+	chr2.2:54657375-54657394	MS.gene65279:intron	35.0%
	ACCATATCTTGCCCACAATT+GGG	-	chr2.2:54657123-54657142	None:intergenic	40.0%
	ACTGCATTCACGGATCAATA+TGG	-	chr2.2:54657090-54657109	None:intergenic	40.0%
	ATATTGATCCGTGAATGCAG+TGG	+	chr2.2:54657089-54657108	MS.gene65279:CDS	40.0%
	CTGCATTCACGGATCAATAT+GGG	-	chr2.2:54657089-54657108	None:intergenic	40.0%
	CTTCCTACAAATCCCAGTTA+AGG	-	chr2.2:54657228-54657247	None:intergenic	40.0%
	GAATGTGAATGCAAATCACG+TGG	-	chr2.2:54657189-54657208	None:intergenic	40.0%
	GTTCCTTAACTGGGATTTGT+AGG	+	chr2.2:54657222-54657241	MS.gene65279:intron	40.0%
	TACCATATCTTGCCCACAAT+TGG	-	chr2.2:54657124-54657143	None:intergenic	40.0%
!	GGATCAATATGGGCAGTTTT+GGG	-	chr2.2:54657079-54657098	None:intergenic	40.0%
!!	CTAGATTTGGGTGTTGAGAA+AGG	+	chr2.2:54657931-54657950	MS.gene65279:intron	40.0%
!!	TGTTGAGAAAGGCATCAAAC+TGG	+	chr2.2:54657942-54657961	MS.gene65279:CDS	40.0%
	ACTTTCACCCCTTTATCGGT+TGG	+	chr2.2:54657650-54657669	MS.gene65279:intron	45.0%
	CCATATCTTGCCCACAATTG+GGG	-	chr2.2:54657122-54657141	None:intergenic	45.0%
	CTCGAAGATCTGGTAAAAGC+AGG	+	chr2.2:54657997-54658016	MS.gene65279:CDS	45.0%
	GTTCAACTCCACTGCATTCA+CGG	-	chr2.2:54657100-54657119	None:intergenic	45.0%
	TCGAGTGCCTTCAAAATCTG+TGG	-	chr2.2:54657982-54658001	None:intergenic	45.0%
	TGGAGTTGAACCCCAATTGT+GGG	+	chr2.2:54657109-54657128	MS.gene65279:CDS	45.0%
!	CGGATCAATATGGGCAGTTT+TGG	-	chr2.2:54657080-54657099	None:intergenic	45.0%
!	GACAGAGCCACAGATTTTGA+AGG	+	chr2.2:54657972-54657991	MS.gene65279:CDS	45.0%
!!	GAGAAAGGCATCAAACTGGA+AGG	+	chr2.2:54657946-54657965	MS.gene65279:CDS	45.0%
!!	TTTGAAGGCACTCGAAGATC+TGG	+	chr2.2:54657987-54658006	MS.gene65279:CDS	45.0%
!!!	ATAATATTTAAAATTAGTTT+TGG	-	chr2.2:54657531-54657550	None:intergenic	5.0%
	AACCTTGCAGATGAGCTTGC+AGG	-	chr2.2:54656890-54656909	None:intergenic	50.0%
	ATGAGCTTGCAGGTGATGAC+TGG	-	chr2.2:54656880-54656899	None:intergenic	50.0%
	CCCCAATTGTGGGCAAGATA+TGG	+	chr2.2:54657119-54657138	MS.gene65279:CDS	50.0%
	GTGGAGTTGAACCCCAATTG+TGG	+	chr2.2:54657108-54657127	MS.gene65279:CDS	50.0%
	AAGGCGTAGCCTAGTGAGTC+GGG	-	chr2.2:54657474-54657493	None:intergenic	55.0%
	CACCTGCAAGCTCATCTGCA+AGG	+	chr2.2:54656885-54656904	MS.gene65279:CDS	55.0%
	GTAGCCTAGTGAGTCGGGTT+GGG	-	chr2.2:54657469-54657488	None:intergenic	55.0%
!	GAAGGCGTAGCCTAGTGAGT+CGG	-	chr2.2:54657475-54657494	None:intergenic	55.0%
	CGTAGCCTAGTGAGTCGGGT+TGG	-	chr2.2:54657470-54657489	None:intergenic	60.0%
	GTGTCCCAACCCGACTCACT+AGG	+	chr2.2:54657462-54657481	MS.gene65279:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	54656821	54658035	54656821	ID=MS.gene65279
chr2.2	mRNA	54656821	54658035	54656821	ID=MS.gene65279.t1;Parent=MS.gene65279
chr2.2	exon	54656821	54656906	54656821	ID=MS.gene65279.t1.exon1;Parent=MS.gene65279.t1
chr2.2	CDS	54656821	54656906	54656821	ID=cds.MS.gene65279.t1;Parent=MS.gene65279.t1
chr2.2	exon	54657034	54657140	54657034	ID=MS.gene65279.t1.exon2;Parent=MS.gene65279.t1
chr2.2	CDS	54657034	54657140	54657034	ID=cds.MS.gene65279.t1;Parent=MS.gene65279.t1
chr2.2	exon	54657935	54658035	54657935	ID=MS.gene65279.t1.exon3;Parent=MS.gene65279.t1
chr2.2	CDS	54657935	54658035	54657935	ID=cds.MS.gene65279.t1;Parent=MS.gene65279.t1

Gene Sequence

>MS.gene65279

ATGGCATGGAGAGGACAACTTTCAAAGAACATCAAAGAGCTTCGATTTCTGATGTGCCAGTCATCACCTGCAAGCTCATCTGCAAGGTTATATATATTTTTTCCCCTATCTTTGAACCTAAAATTTCATAGCATTAATTTTTAAAAATCTTTTTATTAAATTTCGTACATACTTGAATCTTTAATGAGCTCTTACTATTTTTTCTTATCTCAGGGCTTTTGTTGAAAAGAATTATAAGGAACTAAAGACATTGAACCCAAAACTGCCCATATTGATCCGTGAATGCAGTGGAGTTGAACCCCAATTGTGGGCAAGATATGGTATCGCCCATCTTAAATCTCTTTCTTTTTACTTTGCATTTCATACCACGTGATTTGCATTCACATTCCATATTCTCCTTAGTTCCTTAACTGGGATTTGTAGGAAGTAAACATAATGCTTTTACACTGAAAATATGCTTCTTCTTCTTCTTTTTGTTTTTTTTTTTACAAGAACACTCAAAATATGATAATCAGCATTCTTCCCTTAAATTCATTTCAATGTTAAAGGCCAATAACTCAAAGCATTCTTTTGGTGGTTAATTTGTTAGAGGGAGATAAATTTGTTAGTATCTGGATCTCATCATGTATAGTGTTCTTTTGGTGTCCCAACCCGACTCACTAGGCTACGCCTTCAGAGATGAGAATATTATAGTATGAGTAGTCTGTCCAAAACTAATTTTAAATATTATATCAATTTTGACTTTTATCTATGAACATTTGTTTGTATGAATCGCAAGGAGTCTGATATCCTTCAAGTATTAATGAATGGAAAAATTGGAGTTGGAAGAACTTTCACCCCTTTATCGGTTGGTTAAAAACAACTTGACCCAATTAGAAGTTGTGTATAATGTCAATTCGGTTTTAATTTTTACTTAAGATTATTTTGCGTATTGTGGATGGCCTTGAATGCATAAAGAAAATTAAGCCACATACTGTAAATTTTTGTATACTTACTCTTCAAAGATGGTTCACATTTCTGATCATATCTTGTAGCTCATCCTTCTCATCTGCATTATTTTGTTTATCTATTTGTGATATATCCTAACATCCTATAATTTAATCATTAATACTAGATTTGGGTGTTGAGAAAGGCATCAAACTGGAAGGCATGACAGAGCCACAGATTTTGAAGGCACTCGAAGATCTGGTAAAAGCAGGACAATCCAAAGCTTGA

Protein sequence

>MS.gene65279.t1

MAWRGQLSKNIKELRFLMCQSSPASSSARAFVEKNYKELKTLNPKLPILIRECSGVEPQLWARYDLGVEKGIKLEGMTEPQILKALEDLVKAGQSKA