Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66408.t1 | AES77005.2 | 84.9 | 166 | 24 | 1 | 1 | 166 | 7 | 171 | 5.00E-70 | 273.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66408.t1 | G7KI10 | 84.9 | 166 | 24 | 1 | 1 | 166 | 7 | 171 | 3.6e-70 | 273.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049438 | MS.gene66408 | 0.814187 | 1.75E-51 | -1.69E-46 |
| MS.gene050288 | MS.gene66408 | 0.802967 | 4.36E-49 | -1.69E-46 |
| MS.gene050838 | MS.gene66408 | 0.803888 | 2.81E-49 | -1.69E-46 |
| MS.gene051676 | MS.gene66408 | 0.806304 | 8.78E-50 | -1.69E-46 |
| MS.gene051748 | MS.gene66408 | 0.801224 | 9.96E-49 | -1.69E-46 |
| MS.gene051903 | MS.gene66408 | 0.822569 | 2.21E-53 | -1.69E-46 |
| MS.gene052242 | MS.gene66408 | -0.81264 | 3.83E-51 | -1.69E-46 |
| MS.gene052296 | MS.gene66408 | 0.826083 | 3.30E-54 | -1.69E-46 |
| MS.gene052302 | MS.gene66408 | 0.810479 | 1.13E-50 | -1.69E-46 |
| MS.gene052376 | MS.gene66408 | 0.804005 | 2.66E-49 | -1.69E-46 |
| MS.gene052818 | MS.gene66408 | -0.823874 | 1.10E-53 | -1.69E-46 |
| MS.gene052947 | MS.gene66408 | 0.817495 | 3.20E-52 | -1.69E-46 |
| MS.gene053189 | MS.gene66408 | 0.881993 | 1.51E-70 | -1.69E-46 |
| MS.gene053298 | MS.gene66408 | 0.807879 | 4.07E-50 | -1.69E-46 |
| MS.gene053340 | MS.gene66408 | 0.814216 | 1.72E-51 | -1.69E-46 |
| MS.gene053564 | MS.gene66408 | 0.802013 | 6.86E-49 | -1.69E-46 |
| MS.gene053963 | MS.gene66408 | 0.821638 | 3.63E-53 | -1.69E-46 |
| MS.gene054222 | MS.gene66408 | 0.801951 | 7.06E-49 | -1.69E-46 |
| MS.gene054536 | MS.gene66408 | 0.84535 | 4.29E-59 | -1.69E-46 |
| MS.gene054699 | MS.gene66408 | 0.803848 | 2.87E-49 | -1.69E-46 |
| MS.gene05469 | MS.gene66408 | 0.810461 | 1.14E-50 | -1.69E-46 |
| MS.gene056830 | MS.gene66408 | -0.811368 | 7.25E-51 | -1.69E-46 |
| MS.gene057451 | MS.gene66408 | -0.801495 | 8.77E-49 | -1.69E-46 |
| MS.gene057452 | MS.gene66408 | -0.819335 | 1.23E-52 | -1.69E-46 |
| MS.gene057890 | MS.gene66408 | -0.819139 | 1.36E-52 | -1.69E-46 |
| MS.gene057999 | MS.gene66408 | 0.831682 | 1.45E-55 | -1.69E-46 |
| MS.gene058074 | MS.gene66408 | 0.8685 | 6.24E-66 | -1.69E-46 |
| MS.gene058238 | MS.gene66408 | 0.812473 | 4.17E-51 | -1.69E-46 |
| MS.gene059872 | MS.gene66408 | -0.807583 | 4.71E-50 | -1.69E-46 |
| MS.gene06011 | MS.gene66408 | 0.800594 | 1.34E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66408.t1 | MTR_6g090460 | 95.783 | 166 | 6 | 1 | 1 | 166 | 7 | 171 | 2.29e-113 | 319 |
| MS.gene66408.t1 | MTR_8g102350 | 49.020 | 153 | 57 | 5 | 4 | 141 | 8 | 154 | 1.44e-24 | 93.6 |
| MS.gene66408.t1 | MTR_3g064350 | 52.174 | 92 | 31 | 2 | 59 | 150 | 76 | 154 | 1.74e-21 | 86.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66408.t1 | AT5G56550 | 58.427 | 89 | 33 | 2 | 56 | 140 | 72 | 160 | 1.70e-24 | 94.0 |
| MS.gene66408.t1 | AT4G26288 | 51.887 | 106 | 30 | 5 | 41 | 138 | 40 | 132 | 5.66e-16 | 71.2 |
Find 21 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTAATAAAGATGAACTTAA+AGG | 0.150671 | 6.3:-82734033 | None:intergenic |
| GATGAAATTGCATACTTTAT+AGG | 0.231922 | 6.3:-82732997 | None:intergenic |
| CCTTTAAGTTCATCTTTATT+AGG | 0.260098 | 6.3:+82734033 | MS.gene66408:CDS |
| ATGAAGTCATGTAGAAGTTA+TGG | 0.374322 | 6.3:+82733940 | MS.gene66408:CDS |
| TAGAAGTTATGGAGGAGGTT+TGG | 0.410155 | 6.3:+82733951 | MS.gene66408:CDS |
| GTTTGGATAATCATAGAATA+TGG | 0.415366 | 6.3:+82733968 | MS.gene66408:CDS |
| TCTTCATCATCACATTCAAA+TGG | 0.424478 | 6.3:+82732937 | MS.gene66408:CDS |
| TTCTTGTGTACAACAATTGA+AGG | 0.439362 | 6.3:-82734087 | None:intergenic |
| AAACATGAAGTGGATGGTTA+AGG | 0.446369 | 6.3:+82732810 | MS.gene66408:CDS |
| TCATCTTTATTAGGCAAAAG+AGG | 0.482739 | 6.3:+82734042 | MS.gene66408:CDS |
| CATCTTTATTAGGCAAAAGA+GGG | 0.516192 | 6.3:+82734043 | MS.gene66408:CDS |
| AGTGATTCAACAACATCCAT+TGG | 0.529919 | 6.3:+82732841 | MS.gene66408:CDS |
| TCATGTAGAAGTTATGGAGG+AGG | 0.533647 | 6.3:+82733946 | MS.gene66408:CDS |
| ACAACAATTGAAGGTCTAGA+AGG | 0.538516 | 6.3:-82734078 | None:intergenic |
| ATCTTTCACCTCTTTAGCAG+AGG | 0.547544 | 6.3:+82733867 | MS.gene66408:CDS |
| AAGTCATGTAGAAGTTATGG+AGG | 0.581965 | 6.3:+82733943 | MS.gene66408:CDS |
| TAGAATATGGTATAGTCCAA+AGG | 0.592131 | 6.3:+82733981 | MS.gene66408:CDS |
| ATCTTCTGAGAATGTTCCAA+TGG | 0.596281 | 6.3:-82732857 | None:intergenic |
| TGCAAGAAACATGAAGTGGA+TGG | 0.612939 | 6.3:+82732804 | MS.gene66408:CDS |
| ATAGTGCAAGAAACATGAAG+TGG | 0.643670 | 6.3:+82732800 | MS.gene66408:CDS |
| TTGTTCATCAGATTTGACTG+AGG | 0.665115 | 6.3:+82732900 | MS.gene66408:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAAAACTTATAAAAATGA+AGG | - | chr6.3:82733022-82733041 | None:intergenic | 10.0% |
| !!! | TATAAGTTTTATTCAAAAAA+GGG | + | chr6.3:82733029-82733048 | MS.gene66408:intron | 10.0% |
| !!! | TTATAAGTTTTATTCAAAAA+AGG | + | chr6.3:82733028-82733047 | MS.gene66408:intron | 10.0% |
| !! | AAAGAACTAATTAATATGTA+CGG | + | chr6.3:82733432-82733451 | MS.gene66408:intron | 15.0% |
| !! | TAAACTAATAATTACGTAAA+AGG | + | chr6.3:82733511-82733530 | MS.gene66408:intron | 15.0% |
| !!! | TAATTTTTACTTTTTAGATG+TGG | + | chr6.3:82733762-82733781 | MS.gene66408:intron | 15.0% |
| !! | TTATATCAATCTATCTATCA+CGG | - | chr6.3:82733731-82733750 | None:intergenic | 20.0% |
| !!! | AACAATTTTCTTGACAAAAA+TGG | - | chr6.3:82733578-82733597 | None:intergenic | 20.0% |
| !!! | TTTTGCATCTCAATAATTTT+AGG | - | chr6.3:82733474-82733493 | None:intergenic | 20.0% |
| ! | ACCAAAAACTAATCTAAACA+AGG | - | chr6.3:82733317-82733336 | None:intergenic | 25.0% |
| ! | ATAAAAGACACCTTCATAAA+AGG | - | chr6.3:82733070-82733089 | None:intergenic | 25.0% |
| ! | ATATCAAAAAAAGCTTCAAG+AGG | + | chr6.3:82734009-82734028 | MS.gene66408:CDS | 25.0% |
| ! | CATAATTGAGTTTGATTCTT+CGG | - | chr6.3:82733397-82733416 | None:intergenic | 25.0% |
| ! | CATACATATTTGTCAAACTT+AGG | - | chr6.3:82733294-82733313 | None:intergenic | 25.0% |
| ! | CCTAATAAAGATGAACTTAA+AGG | - | chr6.3:82734036-82734055 | None:intergenic | 25.0% |
| ! | GATGAAATTGCATACTTTAT+AGG | - | chr6.3:82733000-82733019 | None:intergenic | 25.0% |
| ! | GTAATTATCTAGATCTATGA+AGG | - | chr6.3:82733702-82733721 | None:intergenic | 25.0% |
| ! | GTTTGGATAATCATAGAATA+TGG | + | chr6.3:82733968-82733987 | MS.gene66408:CDS | 25.0% |
| ! | TATCAAAAAAAGCTTCAAGA+GGG | + | chr6.3:82734010-82734029 | MS.gene66408:CDS | 25.0% |
| ! | TGTCAAGAAAATTGTTACAT+AGG | + | chr6.3:82733582-82733601 | MS.gene66408:intron | 25.0% |
| !! | CCTTTAAGTTCATCTTTATT+AGG | + | chr6.3:82734033-82734052 | MS.gene66408:CDS | 25.0% |
| !!! | AATTAGTTTATGACTTTTGC+AGG | + | chr6.3:82733809-82733828 | MS.gene66408:intron | 25.0% |
| !!! | AGTAGTGACATTTTTTTTGA+AGG | - | chr6.3:82733236-82733255 | None:intergenic | 25.0% |
| !!! | GTAGTGACATTTTTTTTGAA+GGG | - | chr6.3:82733235-82733254 | None:intergenic | 25.0% |
| !!! | TTTTTTGATATTGTAGCCTT+TGG | - | chr6.3:82734000-82734019 | None:intergenic | 25.0% |
| AAAGTGAAAATCACACCTTA+AGG | + | chr6.3:82733167-82733186 | MS.gene66408:intron | 30.0% | |
| ATGAAGTCATGTAGAAGTTA+TGG | + | chr6.3:82733940-82733959 | MS.gene66408:CDS | 30.0% | |
| CATCTTTATTAGGCAAAAGA+GGG | + | chr6.3:82734043-82734062 | MS.gene66408:CDS | 30.0% | |
| TAGAATATGGTATAGTCCAA+AGG | + | chr6.3:82733981-82734000 | MS.gene66408:CDS | 30.0% | |
| TCATCTTTATTAGGCAAAAG+AGG | + | chr6.3:82734042-82734061 | MS.gene66408:CDS | 30.0% | |
| TCTTCATCATCACATTCAAA+TGG | + | chr6.3:82732937-82732956 | MS.gene66408:CDS | 30.0% | |
| TTCTTGTGTACAACAATTGA+AGG | - | chr6.3:82734090-82734109 | None:intergenic | 30.0% | |
| ! | ACTTTTTAGATGTGGTGAAT+GGG | + | chr6.3:82733770-82733789 | MS.gene66408:intron | 30.0% |
| ! | ATAGAAGACACCTTTTATGA+AGG | + | chr6.3:82733057-82733076 | MS.gene66408:intron | 30.0% |
| ! | GGTTGTCAATGTTTTATCAA+GGG | + | chr6.3:82733836-82733855 | MS.gene66408:CDS | 30.0% |
| ! | TTGTAGGATATGCTTTTCTT+TGG | - | chr6.3:82733913-82733932 | None:intergenic | 30.0% |
| !! | AAAGACACCAACTTACTTTT+AGG | + | chr6.3:82733115-82733134 | MS.gene66408:intron | 30.0% |
| !! | TACTTTTTAGATGTGGTGAA+TGG | + | chr6.3:82733769-82733788 | MS.gene66408:intron | 30.0% |
| !! | TGACTTCATTCTTTTGTTGT+AGG | - | chr6.3:82733929-82733948 | None:intergenic | 30.0% |
| !!! | GCCTTGTTTAGATTAGTTTT+TGG | + | chr6.3:82733313-82733332 | MS.gene66408:intron | 30.0% |
| AAACATGAAGTGGATGGTTA+AGG | + | chr6.3:82732810-82732829 | MS.gene66408:CDS | 35.0% | |
| AAGTCATGTAGAAGTTATGG+AGG | + | chr6.3:82733943-82733962 | MS.gene66408:CDS | 35.0% | |
| ACAACAATTGAAGGTCTAGA+AGG | - | chr6.3:82734081-82734100 | None:intergenic | 35.0% | |
| ACACCTTCCTAAAAGTAAGT+TGG | - | chr6.3:82733125-82733144 | None:intergenic | 35.0% | |
| AGTGATTCAACAACATCCAT+TGG | + | chr6.3:82732841-82732860 | MS.gene66408:CDS | 35.0% | |
| ATAGTGCAAGAAACATGAAG+TGG | + | chr6.3:82732800-82732819 | MS.gene66408:CDS | 35.0% | |
| TGATAAAAGACACCACCAAA+GGG | + | chr6.3:82733194-82733213 | MS.gene66408:intron | 35.0% | |
| TTGATAAAAGACACCACCAA+AGG | + | chr6.3:82733193-82733212 | MS.gene66408:intron | 35.0% | |
| TTGTTCATCAGATTTGACTG+AGG | + | chr6.3:82732900-82732919 | MS.gene66408:CDS | 35.0% | |
| ! | ATCTTCTGAGAATGTTCCAA+TGG | - | chr6.3:82732860-82732879 | None:intergenic | 35.0% |
| ! | GGGTTGTCAATGTTTTATCA+AGG | + | chr6.3:82733835-82733854 | MS.gene66408:CDS | 35.0% |
| ! | GTCAATGTTTTATCAAGGGA+AGG | + | chr6.3:82733840-82733859 | MS.gene66408:CDS | 35.0% |
| ! | GTCTTTTATCAAGCACCTTA+AGG | - | chr6.3:82733185-82733204 | None:intergenic | 35.0% |
| ! | TTTATGACTTTTGCAGGAGA+GGG | + | chr6.3:82733815-82733834 | MS.gene66408:intron | 35.0% |
| !! | ACACCAACTTACTTTTAGGA+AGG | + | chr6.3:82733119-82733138 | MS.gene66408:intron | 35.0% |
| ATCTTTCACCTCTTTAGCAG+AGG | + | chr6.3:82733867-82733886 | MS.gene66408:CDS | 40.0% | |
| TAGAAGTTATGGAGGAGGTT+TGG | + | chr6.3:82733951-82733970 | MS.gene66408:CDS | 40.0% | |
| TCATGTAGAAGTTATGGAGG+AGG | + | chr6.3:82733946-82733965 | MS.gene66408:CDS | 40.0% | |
| TGCAAGAAACATGAAGTGGA+TGG | + | chr6.3:82732804-82732823 | MS.gene66408:CDS | 40.0% | |
| ! | GTTTATGACTTTTGCAGGAG+AGG | + | chr6.3:82733814-82733833 | MS.gene66408:intron | 40.0% |
| ! | GTTTTGTACCTCTGCTAAAG+AGG | - | chr6.3:82733878-82733897 | None:intergenic | 40.0% |
| TAAAAGACACCACCAAAGGG+TGG | + | chr6.3:82733197-82733216 | MS.gene66408:intron | 45.0% | |
| TTATGTTCCAAGCCACCCTT+TGG | - | chr6.3:82733212-82733231 | None:intergenic | 45.0% | |
| TGTTCCAAGCCACCCTTTGG+TGG | - | chr6.3:82733209-82733228 | None:intergenic | 55.0% | |
| GACACCACCAAAGGGTGGCT+TGG | + | chr6.3:82733202-82733221 | MS.gene66408:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 82732790 | 82734116 | 82732790 | ID=MS.gene66408 |
| chr6.3 | mRNA | 82732790 | 82734116 | 82732790 | ID=MS.gene66408.t1;Parent=MS.gene66408 |
| chr6.3 | exon | 82732790 | 82733004 | 82732790 | ID=MS.gene66408.t1.exon1;Parent=MS.gene66408.t1 |
| chr6.3 | CDS | 82732790 | 82733004 | 82732790 | ID=cds.MS.gene66408.t1;Parent=MS.gene66408.t1 |
| chr6.3 | exon | 82733831 | 82734116 | 82733831 | ID=MS.gene66408.t1.exon2;Parent=MS.gene66408.t1 |
| chr6.3 | CDS | 82733831 | 82734116 | 82733831 | ID=cds.MS.gene66408.t1;Parent=MS.gene66408.t1 |
| Gene Sequence |
| Protein sequence |
