AlfalfaGEDB | MS.gene67152

M. sativa cultivar XinJiangDaYe / MS.gene67152

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67152.t1	KEH40067.1	72.3	65	17	1	1	64	232	296	1.40E-12	81.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67152.t1	A0A072VE24	72.3	65	17	1	1	64	232	296	1.0e-12	81.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049669	MS.gene67152	0.811302	7.49E-51	-1.69E-46
MS.gene049873	MS.gene67152	0.809786	1.59E-50	-1.69E-46
MS.gene050026	MS.gene67152	0.804049	2.60E-49	-1.69E-46
MS.gene050227	MS.gene67152	0.816815	4.55E-52	-1.69E-46
MS.gene050228	MS.gene67152	0.825978	3.49E-54	-1.69E-46
MS.gene050303	MS.gene67152	0.81663	5.01E-52	-1.69E-46
MS.gene050442	MS.gene67152	0.811175	7.99E-51	-1.69E-46
MS.gene050719	MS.gene67152	0.81039	1.18E-50	-1.69E-46
MS.gene050721	MS.gene67152	0.808335	3.26E-50	-1.69E-46
MS.gene050953	MS.gene67152	0.808371	3.20E-50	-1.69E-46
MS.gene052176	MS.gene67152	0.804929	1.71E-49	-1.69E-46
MS.gene052190	MS.gene67152	0.805836	1.10E-49	-1.69E-46
MS.gene052333	MS.gene67152	0.801976	6.98E-49	-1.69E-46
MS.gene052519	MS.gene67152	0.822387	2.43E-53	-1.69E-46
MS.gene052582	MS.gene67152	0.807666	4.52E-50	-1.69E-46
MS.gene052584	MS.gene67152	0.800269	1.56E-48	-1.69E-46
MS.gene052586	MS.gene67152	0.800708	1.27E-48	-1.69E-46
MS.gene052867	MS.gene67152	0.805118	1.56E-49	-1.69E-46
MS.gene053006	MS.gene67152	0.835324	1.79E-56	-1.69E-46
MS.gene053166	MS.gene67152	0.810009	1.43E-50	-1.69E-46
MS.gene053226	MS.gene67152	0.803772	2.97E-49	-1.69E-46
MS.gene053966	MS.gene67152	0.800765	1.24E-48	-1.69E-46
MS.gene05414	MS.gene67152	0.809515	1.82E-50	-1.69E-46
MS.gene05415	MS.gene67152	0.832465	9.31E-56	-1.69E-46
MS.gene054160	MS.gene67152	0.816369	5.73E-52	-1.69E-46
MS.gene054405	MS.gene67152	0.801358	9.35E-49	-1.69E-46
MS.gene054657	MS.gene67152	0.805396	1.36E-49	-1.69E-46
MS.gene055701	MS.gene67152	0.812586	3.94E-51	-1.69E-46
MS.gene055778	MS.gene67152	0.806915	6.52E-50	-1.69E-46
MS.gene055808	MS.gene67152	0.80432	2.29E-49	-1.69E-46
MS.gene055818	MS.gene67152	0.809867	1.53E-50	-1.69E-46
MS.gene056162	MS.gene67152	0.804483	2.11E-49	-1.69E-46
MS.gene056506	MS.gene67152	0.854734	1.01E-61	-1.69E-46
MS.gene057026	MS.gene67152	0.838023	3.68E-57	-1.69E-46
MS.gene057060	MS.gene67152	0.847008	1.52E-59	-1.69E-46
MS.gene057414	MS.gene67152	0.808302	3.31E-50	-1.69E-46
MS.gene057693	MS.gene67152	0.810291	1.24E-50	-1.69E-46
MS.gene05795	MS.gene67152	0.805476	1.31E-49	-1.69E-46
MS.gene058037	MS.gene67152	0.812962	3.25E-51	-1.69E-46
MS.gene058979	MS.gene67152	0.812383	4.36E-51	-1.69E-46
MS.gene059382	MS.gene67152	0.817031	4.07E-52	-1.69E-46
MS.gene05954	MS.gene67152	0.833943	3.99E-56	-1.69E-46
MS.gene060322	MS.gene67152	0.810961	8.89E-51	-1.69E-46
MS.gene060521	MS.gene67152	0.811905	5.54E-51	-1.69E-46
MS.gene060572	MS.gene67152	0.856253	3.64E-62	-1.69E-46
MS.gene060683	MS.gene67152	0.810963	8.87E-51	-1.69E-46
MS.gene060685	MS.gene67152	0.803254	3.81E-49	-1.69E-46
MS.gene060781	MS.gene67152	0.817364	3.43E-52	-1.69E-46
MS.gene061040	MS.gene67152	-0.846561	2.01E-59	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 12 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCGCTAGAATTAGCTTCTT+TGG	0.160211	1.2:+3373440	None:intergenic
TTTATTATATTAACAAAATA+TGG	0.193896	1.2:+3372846	None:intergenic
TCGCTAGAATTAGCTTCTTT+GGG	0.334685	1.2:+3373441	None:intergenic
TGTCGGTGGTTCATGTATTC+TGG	0.364370	1.2:-3372814	None:intergenic
CGAAAGAACACTTGATTTGA+GGG	0.397450	1.2:-3373421	MS.gene67152:CDS
GCTTGTAACAAGGAACATTC+TGG	0.435134	1.2:-3373399	MS.gene67152:intron
GCGAAAGAACACTTGATTTG+AGG	0.454066	1.2:-3373422	MS.gene67152:CDS
ATTAACAAAATATGGAAAAG+AGG	0.498134	1.2:+3372854	None:intergenic
ACTGTCAAAGTCATCAAGAA+AGG	0.524538	1.2:-3372907	MS.gene67152:CDS
CTAGAATTAGCTTCTTTGGG+TGG	0.573009	1.2:+3373444	None:intergenic
TGATTTGAGGGCTTGTAACA+AGG	0.585553	1.2:-3373409	MS.gene67152:CDS
AACAAAATATGGAAAAGAGG+AGG	0.616204	1.2:+3372857	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTTTTTTTTGTCAAAT+TGG	-	chr1.2:3373244-3373263	MS.gene67152:intron	10.0%
!!	TTTGACAAAAAAAAAAAAAG+TGG	+	chr1.2:3373243-3373262	None:intergenic	15.0%
!!	AAATAATCAAAACTGATCAT+AGG	+	chr1.2:3373186-3373205	None:intergenic	20.0%
!!	AATAATCAAAACTGATCATA+GGG	+	chr1.2:3373185-3373204	None:intergenic	20.0%
!!	AATGATAAAACAAAAGTGAT+TGG	+	chr1.2:3373275-3373294	None:intergenic	20.0%
!!	ATAATAAAAAAGTTCAGTGT+CGG	-	chr1.2:3373433-3373452	MS.gene67152:CDS	20.0%
!!	ATTAACAAAATATGGAAAAG+AGG	+	chr1.2:3373413-3373432	None:intergenic	20.0%
	AACAAAATATGGAAAAGAGG+AGG	+	chr1.2:3373410-3373429	None:intergenic	30.0%
	ACATGTAGTGTGTATCAATA+TGG	-	chr1.2:3373132-3373151	MS.gene67152:intron	30.0%
	ATCTGGATTTGATACTAAAC+TGG	-	chr1.2:3373094-3373113	MS.gene67152:intron	30.0%
	CTTAGGACCATTATAAAAAG+GGG	+	chr1.2:3373037-3373056	None:intergenic	30.0%
	GCTTAGGACCATTATAAAAA+GGG	+	chr1.2:3373038-3373057	None:intergenic	30.0%
	TGGTTGATTACTCTAAGTAT+AGG	+	chr1.2:3373223-3373242	None:intergenic	30.0%
	TTAGGACCATTATAAAAAGG+GGG	+	chr1.2:3373036-3373055	None:intergenic	30.0%
	TTATCAGTATCAAATGACAC+AGG	-	chr1.2:3373063-3373082	MS.gene67152:intron	30.0%
	AAAACTGATCATAGGGAATC+AGG	+	chr1.2:3373178-3373197	None:intergenic	35.0%
	AATTGTTCAATTCCACACAG+AGG	-	chr1.2:3373330-3373349	MS.gene67152:intron	35.0%
	ACTACATGTTCGTTCAGATT+TGG	+	chr1.2:3373121-3373140	None:intergenic	35.0%
	ACTATAACATTCTGATGCCA+TGG	-	chr1.2:3372946-3372965	MS.gene67152:intron	35.0%
	ACTGTCAAAGTCATCAAGAA+AGG	-	chr1.2:3373357-3373376	MS.gene67152:intron	35.0%
	CGCTTAGGACCATTATAAAA+AGG	+	chr1.2:3373039-3373058	None:intergenic	35.0%
	CTATAACATTCTGATGCCAT+GGG	-	chr1.2:3372947-3372966	MS.gene67152:intron	35.0%
	GATACTGATAAAACACGCTT+AGG	+	chr1.2:3373054-3373073	None:intergenic	35.0%
	TAGGACCATTATAAAAAGGG+GGG	+	chr1.2:3373035-3373054	None:intergenic	35.0%
!	ATAAAAAAGTTCAGTGTCGG+TGG	-	chr1.2:3373436-3373455	MS.gene67152:CDS	35.0%
!	CGAAAGAACACTTGATTTGA+GGG	-	chr1.2:3372843-3372862	MS.gene67152:CDS	35.0%
!	TCGCTAGAATTAGCTTCTTT+GGG	+	chr1.2:3372826-3372845	None:intergenic	35.0%
!	TTCGCTAGAATTAGCTTCTT+TGG	+	chr1.2:3372827-3372846	None:intergenic	35.0%
	GCTTGTAACAAGGAACATTC+TGG	-	chr1.2:3372865-3372884	MS.gene67152:CDS	40.0%
	TGATTTGAGGGCTTGTAACA+AGG	-	chr1.2:3372855-3372874	MS.gene67152:CDS	40.0%
	TGTGCAGCATAAAAATCCCA+TGG	+	chr1.2:3372966-3372985	None:intergenic	40.0%
!	CATGTCCCCCCTTTTTATAA+TGG	-	chr1.2:3373027-3373046	MS.gene67152:intron	40.0%
!	CTAGAATTAGCTTCTTTGGG+TGG	+	chr1.2:3372823-3372842	None:intergenic	40.0%
!	GCGAAAGAACACTTGATTTG+AGG	-	chr1.2:3372842-3372861	MS.gene67152:CDS	40.0%
	TGTCGGTGGTTCATGTATTC+TGG	-	chr1.2:3373450-3373469	MS.gene67152:CDS	45.0%
!!	TTTATTATATTAACAAAATA+TGG	+	chr1.2:3373421-3373440	None:intergenic	5.0%
	TGACACAGGCGAGTCGTATC+TGG	-	chr1.2:3373077-3373096	MS.gene67152:intron	55.0%
	TTGACAGTGCTGCCTCTGTG+TGG	+	chr1.2:3373345-3373364	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	3372815	3373471	3372815	ID=MS.gene67152
chr1.2	mRNA	3372815	3373471	3372815	ID=MS.gene67152.t1;Parent=MS.gene67152
chr1.2	exon	3373400	3373471	3373400	ID=MS.gene67152.t1.exon1;Parent=MS.gene67152.t1
chr1.2	CDS	3373400	3373471	3373400	ID=cds.MS.gene67152.t1;Parent=MS.gene67152.t1
chr1.2	exon	3372815	3372936	3372815	ID=MS.gene67152.t1.exon2;Parent=MS.gene67152.t1
chr1.2	CDS	3372815	3372936	3372815	ID=cds.MS.gene67152.t1;Parent=MS.gene67152.t1

Gene Sequence

>MS.gene67152

GAAGACCACCCAAAGAAGCTAATTCTAGCGAAAGAACACTTGATTTGAGGGCTTGTAACAAGGAACATTCTGGTCAAAACATTTCTCTTTTTCTATTAAGTTTTACTATATTAATTCTAGCTTATTTAGTAACTATAACATTCTGATGCCATGGGATTTTTATGCTGCACATGCTTTGCTTTGAAGTTTGTAAAAGCTTAAGAAGATAACTGCATGTCCCCCCTTTTTATAATGGTCCTAAGCGTGTTTTATCAGTATCAAATGACACAGGCGAGTCGTATCTGGATTTGATACTAAACTGGACCAAATCTGAACGAACATGTAGTGTGTATCAATATGGACATGAATCTCATCTTGAATCCTGATTCCCTATGATCAGTTTTGATTATTTCTAATGAATCAGTACCTATACTTAGAGTAATCAACCACTTTTTTTTTTTTTGTCAAATTGGTATAACCAATCACTTTTGTTTTATCATTTGATTTATTACTTCAATTAAAAAAACTCATGATTGAATTGTTCAATTCCACACAGAGGCAGCACTGTCAAAGTCATCAAGAAAGGATGAGAAAAAGATAGCACAATCAGTTACCTCCTCTTTTCCATATTTTGTTAATATAATAAAAAAGTTCAGTGTCGGTGGTTCATGTATTCTG

Protein sequence

>MS.gene67152.t1

EDHPKKLILAKEHLIUGLVTRNILRQHCQSHQERMRKRUHNQLPPLFHILLIUUKSSVSVVHVF