AlfalfaGEDB | MS.gene71256

M. sativa cultivar XinJiangDaYe / MS.gene71256

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914
PFAM: PF01535.23 PF12854.10 PF13041.9 PF14432.9 PF20430.1 PF20431.1

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71256.t1	KEH17467.1	97	558	17	0	1	558	1	558	0.00E+00	1099.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71256.t1	Q9FX24	59.4	569	214	5	1	558	19	581	7.4e-191	668.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71256.t1	A0A072TJ58	97.0	558	17	0	1	558	1	558	0.0e+00	1099.3

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene000626	MS.gene71256	PPI
MS.gene004000	MS.gene71256	PPI
MS.gene016068	MS.gene71256	PPI
MS.gene016069	MS.gene71256	PPI
MS.gene016071	MS.gene71256	PPI
MS.gene017123	MS.gene71256	PPI
MS.gene021157	MS.gene71256	PPI
MS.gene028001	MS.gene71256	PPI
MS.gene033120	MS.gene71256	PPI
MS.gene035805	MS.gene71256	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71256.t1	MTR_0013s0120	96.953	558	17	0	1	558	1	558	0.0	1129
MS.gene71256.t1	MTR_2g018870	42.397	559	320	2	1	558	56	613	4.06e-169	493
MS.gene71256.t1	MTR_2g042550	41.897	580	311	8	1	558	165	740	2.30e-151	452
MS.gene71256.t1	MTR_5g040920	40.107	561	317	5	1	558	42	586	7.03e-151	446
MS.gene71256.t1	MTR_1g012380	40.592	574	318	10	1	558	34	600	2.51e-150	445
MS.gene71256.t1	MTR_1g012380	24.911	281	198	5	100	369	30	308	4.67e-21	97.4
MS.gene71256.t1	MTR_0493s0010	38.935	601	307	9	2	558	27	611	5.98e-147	436
MS.gene71256.t1	MTR_1g059280	38.544	563	334	6	2	558	60	616	1.60e-144	430
MS.gene71256.t1	MTR_1g059280	27.698	278	199	2	92	369	47	322	4.49e-30	125
MS.gene71256.t1	MTR_1g059720	38.929	560	336	5	1	558	19	574	9.24e-143	424

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71256.t1	AT1G34160	59.402	569	214	5	1	558	19	581	0.0	682
MS.gene71256.t1	AT1G34160	57.358	530	203	6	45	558	12	534	0.0	602
MS.gene71256.t1	AT1G34160	25.600	250	177	4	21	268	101	343	2.78e-24	107
MS.gene71256.t1	AT4G21065	42.644	537	303	5	26	558	60	595	1.05e-167	489
MS.gene71256.t1	AT4G21065	45.815	454	243	3	107	558	10	462	1.72e-157	458
MS.gene71256.t1	AT4G21065	29.084	251	174	3	20	268	26	274	9.01e-33	131
MS.gene71256.t1	AT4G21065	30.488	164	112	2	199	362	1	162	2.05e-17	85.5
MS.gene71256.t1	AT3G56550	40.320	563	329	7	1	558	21	581	3.16e-154	454
MS.gene71256.t1	AT3G56550	40.320	563	329	7	1	558	21	581	3.16e-154	454
MS.gene71256.t1	AT5G48910	38.916	609	320	5	1	558	39	646	1.07e-147	439

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Find 145 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAGGGAGAAATTGTTTA+TGG	0.167075	4.2:-63963380	MS.gene71256:CDS
AAACTTAATGTTAAGCATTA+TGG	0.186435	4.2:-63963029	MS.gene71256:CDS
AAATGTGGAAATGGCCGAGA+TGG	0.621857	4.2:-63962880	MS.gene71256:CDS
AAATTATCATAGTGAGAAGT+TGG	0.397800	4.2:-63962526	MS.gene71256:CDS
AACAAAGCTATAGCTTCATC+AGG	0.473916	4.2:+63963488	None:intergenic
AACAGGGAGAAATTGTTTAT+GGG	0.394559	4.2:-63963379	MS.gene71256:CDS
AACCAACACAGTCCATTTCA+TGG	0.417763	4.2:-63963793	MS.gene71256:CDS
AACTTAATGTTAAGCATTAT+GGG	0.361930	4.2:-63963028	MS.gene71256:CDS
AACTTATGGAAATGTGGAAA+TGG	0.428381	4.2:-63962889	MS.gene71256:CDS
AAGAGACGGAGAAGAGTTCA+AGG	0.470971	4.2:+63963918	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACAGGGAGAAATTGTTTA+TGG	-	chr4.2:63962938-63962957	MS.gene71256:CDS	30.0%
AACAGGGAGAAATTGTTTAT+GGG	-	chr4.2:63962939-63962958	MS.gene71256:CDS	30.0%
AACTTATGGAAATGTGGAAA+TGG	-	chr4.2:63963429-63963448	MS.gene71256:CDS	30.0%
AATCTTGTGTTGCATGATAT+AGG	-	chr4.2:63963745-63963764	MS.gene71256:CDS	30.0%
ATAATAGGGAGATTATTGTG+AGG	-	chr4.2:63963926-63963945	MS.gene71256:CDS	30.0%
CGATTTCACAGATTTAAAGA+TGG	-	chr4.2:63963958-63963977	MS.gene71256:CDS	30.0%
GGTTTAATTAGTACAGTTGA+TGG	-	chr4.2:63963826-63963845	MS.gene71256:CDS	30.0%
TAATAGGGAGATTATTGTGA+GGG	-	chr4.2:63963927-63963946	MS.gene71256:CDS	30.0%
TACTTGGAATACTATGATCA+TGG	-	chr4.2:63963093-63963112	MS.gene71256:CDS	30.0%
TAGATGTTTATGCGAAAACT+GGG	-	chr4.2:63962711-63962730	MS.gene71256:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	63962332	63964008	63962332	ID=MS.gene71256
chr4.2	mRNA	63962332	63964008	63962332	ID=MS.gene71256.t1;Parent=MS.gene71256
chr4.2	exon	63962332	63964008	63962332	ID=MS.gene71256.t1.exon1;Parent=MS.gene71256.t1
chr4.2	CDS	63962332	63964008	63962332	ID=cds.MS.gene71256.t1;Parent=MS.gene71256.t1

Gene Sequence

>MS.gene71256

ATGAAGCAACTTCAAGCTCATCTCATAACCACCGGAAAATTCCAATTCCACCCTTCCCGCACCAAGCTCCTTGAACTCTTCTCCGTCTCTTCCTCCGGCAACCTCTCCTTCGCCGGAAAAATATTCCGGCAAATCCAAAACCCCACCACAAACGACTACAACGCCATTCTTCGAGGCTTTGCTCAAAGCTCCGAACCAACACAGTCCATTTCATGGTACCGTGACATGTTACGTTGCATTCAAAGAGTCGATGCTTTGACATGTTCGTTTGCTCTTAAGGGTTGTGCTCGTGCTTTAGCTTTTTCTGAAGCAACCCAGTTGCATTCTCAGGTTCTGCGTTTTGGATTTGATGCGGATGTTTTGTTACTCACTACTTTGTTAGATGTTTATGCGAAAACTGGGTTTATTGATTATGCACAGAAGGTGTTTGATGAAATGGATCAACGAGATATTGCTTCTTGGAATGCGATGATTTCTGGGTTGGCGCAAGGGAGTCGACCTGATGAAGCTATAGCTTTGTTTAATAGGATGAAGGAAGAGGGATGGAGGCCGAATGATGTGACTGTTCTTGGCGCGCTTTCGGCTTGTTCTCAGTTGGGTGCTTTGAAACAGGGAGAAATTGTTTATGGGTATATTGTGGATGAGAAGTTGGATAGGAATGTGATTGTTTGTAATGCTGTTATCGATATGTTTGCTAAATGTGGGTTTGTTGATAAAGCTTATTCAGTCTTTGAAAGTATGAGTTGTAGGAAAAGTCTTATTACTTGGAATACTATGATCATGGCTTTTGCAATGAATGGTGATGGATATAAAGCGCTTGATCTTTTGGATCGAATGAGTTTAGATGGAACGTGTCCGGATGCAGTGTCGTATCTTGGTGCGTTGTGTGCGTGCAACCATGCTGGGTTGGTGGATGAAGGTGTTAGATTGTTTGATTTGATGAAAGTGTCTGGGGTGAAACTTAATGTTAAGCATTATGGGAGTATGGTTGATTTGTTGGGTCGAGCCGGGAGGCTCAAAGAGGCTTATGAAATTATAAACTCCATGCCTATGTTTCCTGATGTGGTTCTTTGGCAGAGTTTGCTTGGAGCTTGCAAAACTTATGGAAATGTGGAAATGGCCGAGATGGCGTCAAAGAAGCTGGTGGAGATGGGATCTAACAGTTCTGGGGATTTTGTCTTGCTGTCTAACGTTTATGCAGCACAACAAAGGTGGAAGGATGTAGGAAAAGTGAGGGAGGCTATGGTAGATAATGACGTTAGGAAGGTGCCTGGATTTAGTTACACGGAAGTAGATGGTAGGATACACAAGTTTATAAATTGTGATCAGAGTCATCCGAATTCCAAAGAGATATACGCAAAGCTGGATGAGATCAAGTTTAGGATTAAAGAATATGGATATATAGCTGAAACTAATCTTGTGTTGCATGATATAGGCGACGAGGACAAGGAGAATGCTTTAAATTATCATAGTGAGAAGTTGGCTGTGGCTTATGGTTTAATTAGTACAGTTGATGGAACACCAATTCAAGTTATAAAGAACCTTAGAATATGTGCAGATTGTCATGCTTTTATTAAAATTATATCAAATATTTATAATAGGGAGATTATTGTGAGGGATCGGGCGCGATTTCACAGATTTAAAGATGGTGTATGCTCTTGTAGAGATTATTGGTAA

Protein sequence

>MS.gene71256.t1

MKQLQAHLITTGKFQFHPSRTKLLELFSVSSSGNLSFAGKIFRQIQNPTTNDYNAILRGFAQSSEPTQSISWYRDMLRCIQRVDALTCSFALKGCARALAFSEATQLHSQVLRFGFDADVLLLTTLLDVYAKTGFIDYAQKVFDEMDQRDIASWNAMISGLAQGSRPDEAIALFNRMKEEGWRPNDVTVLGALSACSQLGALKQGEIVYGYIVDEKLDRNVIVCNAVIDMFAKCGFVDKAYSVFESMSCRKSLITWNTMIMAFAMNGDGYKALDLLDRMSLDGTCPDAVSYLGALCACNHAGLVDEGVRLFDLMKVSGVKLNVKHYGSMVDLLGRAGRLKEAYEIINSMPMFPDVVLWQSLLGACKTYGNVEMAEMASKKLVEMGSNSSGDFVLLSNVYAAQQRWKDVGKVREAMVDNDVRKVPGFSYTEVDGRIHKFINCDQSHPNSKEIYAKLDEIKFRIKEYGYIAETNLVLHDIGDEDKENALNYHSEKLAVAYGLISTVDGTPIQVIKNLRICADCHAFIKIISNIYNREIIVRDRARFHRFKDGVCSCRDYW