AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene71287


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71287.t1 MTR_3g082970 97.788 226 5 0 1 226 1 226 1.53e-166 458
MS.gene71287.t1 MTR_2g025740 71.875 224 49 2 2 225 23 232 4.18e-110 315
MS.gene71287.t1 MTR_4g133060 70.335 209 62 0 17 225 13 221 4.17e-105 303
MS.gene71287.t1 MTR_4g133060 73.196 194 52 0 32 225 2 195 3.59e-102 294
MS.gene71287.t1 MTR_4g133060 65.366 205 67 1 17 217 13 217 1.12e-90 266
MS.gene71287.t1 MTR_8g097200 67.052 173 57 0 53 225 59 231 8.05e-86 254
MS.gene71287.t1 MTR_3g084940 63.636 176 63 1 52 226 52 227 1.99e-78 235
MS.gene71287.t1 MTR_7g097270 51.337 187 88 3 38 223 33 217 5.89e-63 196
MS.gene71287.t1 MTR_7g097270 49.600 125 60 3 38 161 33 155 4.06e-33 118
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71287.t1 AT3G07880 77.114 201 45 1 25 225 38 237 3.48e-113 324
MS.gene71287.t1 AT1G62450 64.319 213 73 2 15 225 11 222 1.56e-91 268
MS.gene71287.t1 AT1G12070 59.912 227 84 2 1 225 1 222 1.64e-88 261

Find 56 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGAGCAATAACATTGTTTC+TGG 0.193009 3.4:-78130921 MS.gene71287:CDS
CATAGGACCTAGCTGTATTT+TGG 0.248019 3.4:+78131656 None:intergenic
CCAGAGGGTGTGACTTCTTC+TGG 0.286287 3.4:+78130689 None:intergenic
AAATGGAAGGAGCAGCTTCT+TGG 0.366946 3.4:-78131446 MS.gene71287:CDS
TGTTTGGAAGACTGCTGTTA+AGG 0.371169 3.4:-78130880 MS.gene71287:CDS
GAGGAGGAAGAAGATGAATA+TGG 0.374764 3.4:-78131681 MS.gene71287:CDS
GTTTGGAAGACTGCTGTTAA+GGG 0.387643 3.4:-78130879 MS.gene71287:intron
AATGGAAGGAGCAGCTTCTT+GGG 0.389589 3.4:-78131445 MS.gene71287:CDS
TAAAATACACCAATACTGTT+TGG 0.391465 3.4:-78130896 MS.gene71287:CDS
TATAGGACGATGAGAGTCTA+AGG 0.397474 3.4:-78131469 MS.gene71287:intron
GTTCTTCCCATTCCTGAAGA+TGG 0.408171 3.4:-78131011 MS.gene71287:CDS
CCAGAAGAAGTCACACCCTC+TGG 0.416770 3.4:-78130689 MS.gene71287:CDS
AGGCTTGTAAAATTCACTTC+AGG 0.420910 3.4:+78131065 None:intergenic
GATAATGAAAACAAAGAAAC+AGG 0.423638 3.4:-78131789 MS.gene71287:CDS
TCTGGCATCTCATGTGTGTA+AGG 0.427995 3.4:+78130707 None:intergenic
GTGTGTAAGGCTCTGGTTGA+GGG 0.432758 3.4:+78130720 None:intergenic
ATCTCATGTGTGTAAGGCTC+TGG 0.437007 3.4:+78130713 None:intergenic
ACGATGAGAGTCTAAGGAAA+TGG 0.439473 3.4:-78131463 MS.gene71287:CDS
TGAGCAATAACATTGTTTCT+GGG 0.463614 3.4:-78130920 MS.gene71287:CDS
CAAAATACAGCTAGGTCCTA+TGG 0.489028 3.4:-78131655 MS.gene71287:CDS
AGAACACCTTGAGAAAGATA+AGG 0.492651 3.4:-78131622 MS.gene71287:intron
AGAAGATTCACTCGTATGCC+TGG 0.493330 3.4:+78131716 None:intergenic
TGTGTGTAAGGCTCTGGTTG+AGG 0.496739 3.4:+78130719 None:intergenic
TTGTGGTTTACTCTTAAAGA+AGG 0.514851 3.4:-78130975 MS.gene71287:CDS
CACCCTCTGGGATATTTGCT+AGG 0.518348 3.4:-78130676 MS.gene71287:CDS
TCCTGAAGATGGAAAACCAC+AGG 0.525613 3.4:-78131000 MS.gene71287:CDS
TGATGACAATAAATGTTACT+TGG 0.527867 3.4:-78130394 MS.gene71287:CDS
AGCCTTTCTATAATCTCTCC+TGG 0.528718 3.4:-78131047 MS.gene71287:CDS
ATGGAAAACCACAGGGATTG+TGG 0.531586 3.4:-78130992 MS.gene71287:CDS
ACCCTCTGGGATATTTGCTA+GGG 0.538296 3.4:-78130675 MS.gene71287:CDS
TCACTCGTATGCCTGGTAAG+AGG 0.546518 3.4:+78131723 None:intergenic
ACAAAACCTTATCTTTCTCA+AGG 0.549448 3.4:+78131616 None:intergenic
AACATCATGAGCCTCTTACC+AGG 0.549984 3.4:-78131734 MS.gene71287:CDS
ACAGGACAAACTAGTGAGGC+TGG 0.550189 3.4:-78131771 MS.gene71287:CDS
AAGCTGAATTTCAGACGGTA+AGG 0.552089 3.4:+78130948 None:intergenic
ACAATATTCTGCAAGATCAA+AGG 0.554037 3.4:-78130651 MS.gene71287:intron
GATAGCTCAAAAGAGATGCT+TGG 0.554702 3.4:-78130752 MS.gene71287:CDS
GTCGATGTCAACAACATTGG+AGG 0.554851 3.4:-78131419 MS.gene71287:intron
AGCAGTCTTCCAAACAGTAT+TGG 0.558230 3.4:+78130887 None:intergenic
CAGAAGAAGTCACACCCTCT+GGG 0.582116 3.4:-78130688 MS.gene71287:CDS
TACCAGGAGAGATTATAGAA+AGG 0.583890 3.4:+78131045 None:intergenic
TATGGAGCCAAAATACAGCT+AGG 0.601518 3.4:-78131663 MS.gene71287:CDS
TGTTCTTTAATAGTGACCAT+AGG 0.603238 3.4:+78131639 None:intergenic
AGTGTCGATGTCAACAACAT+TGG 0.603658 3.4:-78131422 MS.gene71287:CDS
CCCCTAGCAAATATCCCAGA+GGG 0.617735 3.4:+78130674 None:intergenic
TGAGAGTCTAAGGAAATGGA+AGG 0.619544 3.4:-78131459 MS.gene71287:CDS
TTCTGTCTATGCAACTGAGG+AGG 0.624555 3.4:-78131697 MS.gene71287:CDS
AGAACAATGTCATCTCTACC+AGG 0.626041 3.4:+78131029 None:intergenic
CAACAAAGCTGAATTTCAGA+CGG 0.649490 3.4:+78130943 None:intergenic
CCTGAAGATGGAAAACCACA+GGG 0.658329 3.4:-78130999 MS.gene71287:CDS
CCCTCTGGGATATTTGCTAG+GGG 0.670702 3.4:-78130674 MS.gene71287:CDS
AGAGTAAACCACAATCCCTG+TGG 0.676637 3.4:+78130984 None:intergenic
GGACAAACTAGTGAGGCTGG+TGG 0.680791 3.4:-78131768 MS.gene71287:CDS
ATCTTCTGTCTATGCAACTG+AGG 0.702591 3.4:-78131700 MS.gene71287:CDS
AGAAACAGGACAAACTAGTG+AGG 0.703043 3.4:-78131775 MS.gene71287:CDS
TCCCCTAGCAAATATCCCAG+AGG 0.719574 3.4:+78130673 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTGTATAATTTTTTTGT+TGG - chr3.4:78131696-78131715 MS.gene71287:CDS 10.0%
!! GTAATTTAAGTAATTTACTT+AGG + chr3.4:78130606-78130625 None:intergenic 15.0%
!! GTTACCAATAAATAATTATG+AGG + chr3.4:78131607-78131626 None:intergenic 20.0%
!! TGTTCCTCATAATTATTTAT+TGG - chr3.4:78131600-78131619 MS.gene71287:intron 20.0%
!! TTGTTACACTTAATATGTAA+CGG + chr3.4:78131631-78131650 None:intergenic 20.0%
!!! TAAAACAAATGTTATTTCAG+TGG - chr3.4:78131377-78131396 MS.gene71287:intron 20.0%
!!! TCAACTATACTTTTGATATT+AGG - chr3.4:78131783-78131802 MS.gene71287:CDS 20.0%
!!! TGAACAATTTTCTTTTACAA+AGG + chr3.4:78130814-78130833 None:intergenic 20.0%
!!! TGTTCATTGTTTATGTTTTT+GGG - chr3.4:78130646-78130665 MS.gene71287:intron 20.0%
!!! TTGTTCATTGTTTATGTTTT+TGG - chr3.4:78130645-78130664 MS.gene71287:intron 20.0%
! GATAATGAAAACAAAGAAAC+AGG - chr3.4:78130362-78130381 MS.gene71287:CDS 25.0%
! GTGTTACAAAATTGGAAATT+TGG + chr3.4:78131332-78131351 None:intergenic 25.0%
! TAAAATACACCAATACTGTT+TGG - chr3.4:78131255-78131274 MS.gene71287:intron 25.0%
! TGATGACAATAAATGTTACT+TGG - chr3.4:78131757-78131776 MS.gene71287:CDS 25.0%
!! CTATACTTTTGATATTAGGA+AGG - chr3.4:78131787-78131806 MS.gene71287:CDS 25.0%
!! TTTTGATATTAGGAAGGATT+GGG - chr3.4:78131793-78131812 MS.gene71287:CDS 25.0%
!!! AGATAAGGTTTTGTTTCTTT+TGG - chr3.4:78130544-78130563 MS.gene71287:intron 25.0%
ACAAAACCTTATCTTTCTCA+AGG + chr3.4:78130538-78130557 None:intergenic 30.0%
ACAATATTCTGCAAGATCAA+AGG - chr3.4:78131500-78131519 MS.gene71287:intron 30.0%
AGGGATAAGTGTTACAAAAT+TGG + chr3.4:78131340-78131359 None:intergenic 30.0%
TGTTCTTTAATAGTGACCAT+AGG + chr3.4:78130515-78130534 None:intergenic 30.0%
TTGTGGTTTACTCTTAAAGA+AGG - chr3.4:78131176-78131195 MS.gene71287:intron 30.0%
! CATTGGAGGTATATAAATCA+CGG - chr3.4:78130746-78130765 MS.gene71287:CDS 30.0%
! CTTTTGATATTAGGAAGGAT+TGG - chr3.4:78131792-78131811 MS.gene71287:CDS 30.0%
! TATTTTTCTTGTGCAGTTTC+TGG - chr3.4:78131733-78131752 MS.gene71287:CDS 30.0%
! TGAGCAATAACATTGTTTCT+GGG - chr3.4:78131231-78131250 MS.gene71287:intron 30.0%
AGAACACCTTGAGAAAGATA+AGG - chr3.4:78130529-78130548 MS.gene71287:intron 35.0%
AGGCTTGTAAAATTCACTTC+AGG + chr3.4:78131089-78131108 None:intergenic 35.0%
CAACAAAGCTGAATTTCAGA+CGG + chr3.4:78131211-78131230 None:intergenic 35.0%
GTGAGCAATAACATTGTTTC+TGG - chr3.4:78131230-78131249 MS.gene71287:intron 35.0%
TACCAGGAGAGATTATAGAA+AGG + chr3.4:78131109-78131128 None:intergenic 35.0%
TGGGTTGTACGAATGAATAT+AGG - chr3.4:78130665-78130684 MS.gene71287:CDS 35.0%
AAGCTGAATTTCAGACGGTA+AGG + chr3.4:78131206-78131225 None:intergenic 40.0%
ACGATGAGAGTCTAAGGAAA+TGG - chr3.4:78130688-78130707 MS.gene71287:CDS 40.0%
AGAAACAGGACAAACTAGTG+AGG - chr3.4:78130376-78130395 MS.gene71287:CDS 40.0%
AGAACAATGTCATCTCTACC+AGG + chr3.4:78131125-78131144 None:intergenic 40.0%
AGCAGTCTTCCAAACAGTAT+TGG + chr3.4:78131267-78131286 None:intergenic 40.0%
AGCCTTTCTATAATCTCTCC+TGG - chr3.4:78131104-78131123 MS.gene71287:intron 40.0%
ATCTTCTGTCTATGCAACTG+AGG - chr3.4:78130451-78130470 MS.gene71287:intron 40.0%
CAAAATACAGCTAGGTCCTA+TGG - chr3.4:78130496-78130515 MS.gene71287:intron 40.0%
GAGGAGGAAGAAGATGAATA+TGG - chr3.4:78130470-78130489 MS.gene71287:intron 40.0%
GATAGCTCAAAAGAGATGCT+TGG - chr3.4:78131399-78131418 MS.gene71287:intron 40.0%
GTTTGGAAGACTGCTGTTAA+GGG - chr3.4:78131272-78131291 MS.gene71287:intron 40.0%
TATAGGACGATGAGAGTCTA+AGG - chr3.4:78130682-78130701 MS.gene71287:CDS 40.0%
TATGGAGCCAAAATACAGCT+AGG - chr3.4:78130488-78130507 MS.gene71287:intron 40.0%
TGAGAGTCTAAGGAAATGGA+AGG - chr3.4:78130692-78130711 MS.gene71287:CDS 40.0%
TGTTTGGAAGACTGCTGTTA+AGG - chr3.4:78131271-78131290 MS.gene71287:intron 40.0%
! CATAGGACCTAGCTGTATTT+TGG + chr3.4:78130498-78130517 None:intergenic 40.0%
! GGTTTTCCATCTTCAGGAAT+GGG + chr3.4:78131149-78131168 None:intergenic 40.0%
! TGGTTTTCCATCTTCAGGAA+TGG + chr3.4:78131150-78131169 None:intergenic 40.0%
!! AGTGTCGATGTCAACAACAT+TGG - chr3.4:78130729-78130748 MS.gene71287:CDS 40.0%
AAATGGAAGGAGCAGCTTCT+TGG - chr3.4:78130705-78130724 MS.gene71287:CDS 45.0%
AACATCATGAGCCTCTTACC+AGG - chr3.4:78130417-78130436 MS.gene71287:intron 45.0%
AATGGAAGGAGCAGCTTCTT+GGG - chr3.4:78130706-78130725 MS.gene71287:CDS 45.0%
AGAAGATTCACTCGTATGCC+TGG + chr3.4:78130438-78130457 None:intergenic 45.0%
AGAGTAAACCACAATCCCTG+TGG + chr3.4:78131170-78131189 None:intergenic 45.0%
ATCTCATGTGTGTAAGGCTC+TGG + chr3.4:78131441-78131460 None:intergenic 45.0%
ATGGAAAACCACAGGGATTG+TGG - chr3.4:78131159-78131178 MS.gene71287:intron 45.0%
CCTGAAGATGGAAAACCACA+GGG - chr3.4:78131152-78131171 MS.gene71287:intron 45.0%
GTCGATGTCAACAACATTGG+AGG - chr3.4:78130732-78130751 MS.gene71287:CDS 45.0%
GTTCTTCCCATTCCTGAAGA+TGG - chr3.4:78131140-78131159 MS.gene71287:intron 45.0%
TCCTGAAGATGGAAAACCAC+AGG - chr3.4:78131151-78131170 MS.gene71287:intron 45.0%
TCTGGCATCTCATGTGTGTA+AGG + chr3.4:78131447-78131466 None:intergenic 45.0%
TTCTGTCTATGCAACTGAGG+AGG - chr3.4:78130454-78130473 MS.gene71287:intron 45.0%
!! ACAAGTGTGTCGATTGTTGC+AGG - chr3.4:78130967-78130986 MS.gene71287:CDS 45.0%
!! ACCCTCTGGGATATTTGCTA+GGG - chr3.4:78131476-78131495 MS.gene71287:intron 45.0%
!! CAAGTGTGTCGATTGTTGCA+GGG - chr3.4:78130968-78130987 MS.gene71287:CDS 45.0%
!! TAATACAATTAAAATTAAAA+GGG + chr3.4:78131359-78131378 None:intergenic 5.0%
!! TTAATACAATTAAAATTAAA+AGG + chr3.4:78131360-78131379 None:intergenic 5.0%
ACAGGACAAACTAGTGAGGC+TGG - chr3.4:78130380-78130399 MS.gene71287:CDS 50.0%
CAGAAGAAGTCACACCCTCT+GGG - chr3.4:78131463-78131482 MS.gene71287:CDS 50.0%
CCCCTAGCAAATATCCCAGA+GGG + chr3.4:78131480-78131499 None:intergenic 50.0%
TCACTCGTATGCCTGGTAAG+AGG + chr3.4:78130431-78130450 None:intergenic 50.0%
TCCCCTAGCAAATATCCCAG+AGG + chr3.4:78131481-78131500 None:intergenic 50.0%
! CCCTGTGGTTTTCCATCTTC+AGG + chr3.4:78131155-78131174 None:intergenic 50.0%
! GTGTGTAAGGCTCTGGTTGA+GGG + chr3.4:78131434-78131453 None:intergenic 50.0%
! TGTGTGTAAGGCTCTGGTTG+AGG + chr3.4:78131435-78131454 None:intergenic 50.0%
!! CACCCTCTGGGATATTTGCT+AGG - chr3.4:78131475-78131494 MS.gene71287:intron 50.0%
!! CCCTCTGGGATATTTGCTAG+GGG - chr3.4:78131477-78131496 MS.gene71287:intron 50.0%
CCAGAAGAAGTCACACCCTC+TGG - chr3.4:78131462-78131481 MS.gene71287:CDS 55.0%
GGACAAACTAGTGAGGCTGG+TGG - chr3.4:78130383-78130402 MS.gene71287:CDS 55.0%
! CCAGAGGGTGTGACTTCTTC+TGG + chr3.4:78131465-78131484 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 78130353 78131820 78130353 ID=MS.gene71287
chr3.4 mRNA 78130353 78131820 78130353 ID=MS.gene71287.t1;Parent=MS.gene71287
chr3.4 exon 78131623 78131820 78131623 ID=MS.gene71287.t1.exon1;Parent=MS.gene71287.t1
chr3.4 CDS 78131623 78131820 78131623 ID=cds.MS.gene71287.t1;Parent=MS.gene71287.t1
chr3.4 exon 78131420 78131486 78131420 ID=MS.gene71287.t1.exon2;Parent=MS.gene71287.t1
chr3.4 CDS 78131420 78131486 78131420 ID=cds.MS.gene71287.t1;Parent=MS.gene71287.t1
chr3.4 exon 78130880 78131098 78130880 ID=MS.gene71287.t1.exon3;Parent=MS.gene71287.t1
chr3.4 CDS 78130880 78131098 78130880 ID=cds.MS.gene71287.t1;Parent=MS.gene71287.t1
chr3.4 exon 78130652 78130776 78130652 ID=MS.gene71287.t1.exon4;Parent=MS.gene71287.t1
chr3.4 CDS 78130652 78130776 78130652 ID=cds.MS.gene71287.t1;Parent=MS.gene71287.t1
chr3.4 exon 78130353 78130424 78130353 ID=MS.gene71287.t1.exon5;Parent=MS.gene71287.t1
chr3.4 CDS 78130353 78130424 78130353 ID=cds.MS.gene71287.t1;Parent=MS.gene71287.t1
Gene Sequence

>MS.gene71287

ATGGGGTCTGATAATGAAAACAAAGAAACAGGACAAACTAGTGAGGCTGGTGGAGAAAAAGAAGAACATCATGAGCCTCTTACCAGGCATACGAGTGAATCTTCTGTCTATGCAACTGAGGAGGAAGAAGATGAATATGGAGCCAAAATACAGCTAGGTCCTATGGTCACTATTAAAGAACACCTTGAGAAAGATAAGGTTTTGTTTCTTTTGGAACTACACATTTGTCTTGTTAATATAATGTTTAATTCCTAAGTAAATTACTTAAATTACATTTTTCATGCTTTATTTTTTGTTCATTGTTTATGTTTTTGGGTTGTACGAATGAATATAGGACGATGAGAGTCTAAGGAAATGGAAGGAGCAGCTTCTTGGGAGTGTCGATGTCAACAACATTGGAGGTATATAAATCACGGTTGTCTCTTTTATAACACTATCATGTCTGATTACAATTGTCACCTTTGTAAAAGAAAATTGTTCAAAATCAACGTCACTTTCAATCTTTATTGTAATATTAATTATTCTTTTTATAATTATATATTATAATTAATATTGTGTACATTACTCTCAAGTGAATATCAACTATTTACGTTTTTTTAATTTGTGTGAAATAAACAAGTGTGTCGATTGTTGCAGGGCGAATCGAGTATGTGTTTATGCATGATTGATGAATATGTTCATTTTGAGAATTTACTTATTTTTTTCAATTTATTTGTTTGTAGAAATTCTTGAACCTGAAGTGAATTTTACAAGCCTTTCTATAATCTCTCCTGGTAGAGATGACATTGTTCTTCCCATTCCTGAAGATGGAAAACCACAGGGATTGTGGTTTACTCTTAAAGAAGGTAGTCCTTACCGTCTGAAATTCAGCTTTGTTGTGAGCAATAACATTGTTTCTGGGTTAAAATACACCAATACTGTTTGGAAGACTGCTGTTAAGGGTATGTTGAATTTAATTCATATAGTCTAATGTTTTCCAAATTTCCAATTTTGTAACACTTATCCCTTTTAATTTTAATTGTATTAAAACAAATGTTATTTCAGTGGATAGCTCAAAAGAGATGCTTGGAACTTTCAGCCCTCAACCAGAGCCTTACACACATGAGATGCCAGAAGAAGTCACACCCTCTGGGATATTTGCTAGGGGACAATATTCTGCAAGATCAAAGGTAATTTAGATTGCAATTAACTGCACTGATTTTGATTGAACAATTATAACATATATGCACTTTCATAATTAGATGAAATGTTCCTCATAATTATTTATTGGTAACTCCGTTACATATTAAGTGTAACAATTTCTCTCAAAAAAAAAATTAAGTGTAACAATTATTACATTTAAAATATTGTATAATTTTTTTGTTGGAAGATAATGATACATATTTTTCTTGTGCAGTTTCTGGATGATGACAATAAATGTTACTTGGAGATCAACTATACTTTTGATATTAGGAAGGATTGGGCTTGA

Protein sequence

>MS.gene71287.t1

MGSDNENKETGQTSEAGGEKEEHHEPLTRHTSESSVYATEEEEDEYGAKIQLGPMVTIKEHLEKDKDDESLRKWKEQLLGSVDVNNIGEILEPEVNFTSLSIISPGRDDIVLPIPEDGKPQGLWFTLKEGSPYRLKFSFVVSNNIVSGLKYTNTVWKTAVKVDSSKEMLGTFSPQPEPYTHEMPEEVTPSGIFARGQYSARSKFLDDDNKCYLEINYTFDIRKDWA