AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene71830


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71830.t1 MTR_1g110760 75.460 163 5 3 1 131 1 160 1.33e-62 189
MS.gene71830.t1 MTR_6g018430 39.623 159 52 4 2 131 25 168 1.61e-26 97.4
MS.gene71830.t1 MTR_8g104210 40.854 164 42 7 7 131 16 163 8.40e-23 87.8
MS.gene71830.t1 MTR_8g104210 40.854 164 42 7 7 131 25 172 1.31e-22 87.8
MS.gene71830.t1 MTR_6g084470 38.608 158 55 5 5 131 21 167 1.26e-19 79.7
MS.gene71830.t1 MTR_1g051170 47.674 86 38 2 39 117 63 148 1.57e-11 58.5
MS.gene71830.t1 MTR_4g087400 50.000 80 36 2 39 117 59 135 2.84e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71830.t1 AT1G68490 52.469 162 36 5 4 131 29 183 3.85e-37 125
MS.gene71830.t1 AT5G16110 47.500 160 48 5 4 131 89 244 1.68e-29 107
MS.gene71830.t1 AT1G13390 43.478 161 49 5 4 131 25 176 3.62e-28 102
MS.gene71830.t1 AT1G13390 43.478 161 49 5 4 131 25 176 3.62e-28 102
MS.gene71830.t1 AT3G02555 45.695 151 41 4 5 131 29 162 2.14e-24 92.0
MS.gene71830.t1 AT3G02555 45.695 151 41 4 5 131 29 162 2.14e-24 92.0

Find 58 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCCGGATTGTTACCAAAAT+TGG 0.118190 1.3:-75507860 None:intergenic
GGATGTGTTCGGGCCAATTT+TGG 0.234336 1.3:+75507847 MS.gene71830:CDS
AGTCCGACACGGCGGGGCTT+AGG 0.339729 1.3:-75507132 None:intergenic
AGTAGCTAACCCACTAATTC+AGG 0.341828 1.3:+75507714 MS.gene71830:CDS
TAATTCAGGATGCTCGATTC+GGG 0.363565 1.3:+75507728 MS.gene71830:CDS
CCTCCCTTCCTTGCAGAAGA+TGG 0.393816 1.3:-75507826 None:intergenic
CTAATTCAGGATGCTCGATT+CGG 0.401608 1.3:+75507727 MS.gene71830:CDS
AGAAGGTGGCAGACCGGATG+GGG 0.404001 1.3:-75507800 None:intergenic
GAGCAGGGATGCTGCAATTA+CGG 0.410027 1.3:-75507921 None:intergenic
CGCCCCTCTCACCGTCGTCA+TGG 0.419708 1.3:+75507764 MS.gene71830:CDS
GTCCGACACGGCGGGGCTTA+GGG 0.420772 1.3:-75507131 None:intergenic
CAAGGAAGGGAGGATGTGTT+CGG 0.421741 1.3:+75507836 MS.gene71830:CDS
GATCCTTCCGGTGGCATCTC+AGG 0.425952 1.3:+75507187 MS.gene71830:CDS
CTCCCTTCCTTGCAGAAGAT+GGG 0.432493 1.3:-75507825 None:intergenic
AAGGATTCGATTGCCTTGAC+AGG 0.436802 1.3:+75507893 MS.gene71830:CDS
TAATTGCAGCATCCCTGCTC+TGG 0.448656 1.3:+75507924 MS.gene71830:CDS
GCCCCTCTCACCGTCGTCAT+GGG 0.457309 1.3:+75507765 MS.gene71830:CDS
GGAGAAGGTGGCAGACCGGA+TGG 0.458776 1.3:-75507802 None:intergenic
GGTGGCAGACCGGATGGGGC+AGG 0.466438 1.3:-75507796 None:intergenic
TGCCCTAAGCCCCGCCGTGT+CGG 0.473384 1.3:+75507129 MS.gene71830:CDS
CTGTTGTTCTAAGCCAGAGC+AGG 0.481278 1.3:-75507937 None:intergenic
GAGAAGGTGGCAGACCGGAT+GGG 0.484866 1.3:-75507801 None:intergenic
ACTACCCGAGCAGATCCTTC+CGG 0.494170 1.3:+75507175 MS.gene71830:CDS
GTGGTGGTTCCTGCCCCATC+CGG 0.495940 1.3:+75507787 MS.gene71830:CDS
GTTGAGGAGTCCGACACGGC+GGG 0.496709 1.3:-75507139 None:intergenic
CTCACCGTCGTCATGGGTGG+TGG 0.513742 1.3:+75507771 MS.gene71830:CDS
TCTGCTCGGGTAGTCGTTGA+CGG 0.526759 1.3:-75507166 None:intergenic
CCACCCATGACGACGGTGAG+AGG 0.533000 1.3:-75507768 None:intergenic
AATCCGGCGGTGAGAGTCGA+AGG 0.545936 1.3:+75507874 MS.gene71830:CDS
TCCCTTCCTTGCAGAAGATG+GGG 0.546203 1.3:-75507824 None:intergenic
AATCGAGCATCCTGAATTAG+TGG 0.550154 1.3:-75507724 None:intergenic
AGTCGTTGACGGCGAAGTTG+AGG 0.554697 1.3:-75507155 None:intergenic
CACCCATGACGACGGTGAGA+GGG 0.562391 1.3:-75507767 None:intergenic
AAGGAAGGGAGGATGTGTTC+GGG 0.568345 1.3:+75507837 MS.gene71830:CDS
GCCACCGGAAGGATCTGCTC+GGG 0.575084 1.3:-75507179 None:intergenic
CCTCTCACCGTCGTCATGGG+TGG 0.588491 1.3:+75507768 MS.gene71830:CDS
AGTGGGTTAGCTACTCTGCT+CGG 0.596257 1.3:-75507706 None:intergenic
TGCCACCGGAAGGATCTGCT+CGG 0.596625 1.3:-75507180 None:intergenic
AGGATTCGATTGCCTTGACA+GGG 0.597575 1.3:+75507894 MS.gene71830:CDS
CCATCTTCTGCAAGGAAGGG+AGG 0.605126 1.3:+75507826 MS.gene71830:CDS
CCCTTCCTTGCAGAAGATGG+GGG 0.610451 1.3:-75507823 None:intergenic
ACCCATGACGACGGTGAGAG+GGG 0.613698 1.3:-75507766 None:intergenic
AATCCTTCGACTCTCACCGC+CGG 0.616188 1.3:-75507877 None:intergenic
TTTCGACGAAGGAAGATGAA+CGG 0.620932 1.3:+75507090 None:intergenic
AATTACGGCTGTCCCTGTCA+AGG 0.629306 1.3:-75507906 None:intergenic
ATCGAGCATCCTGAATTAGT+GGG 0.630666 1.3:-75507723 None:intergenic
GGCGGCGACGAGGCTACTGG+AGG 0.632101 1.3:-75507661 None:intergenic
AAATTCACCTGAGATGCCAC+CGG 0.634810 1.3:-75507194 None:intergenic
TCACCTGAGATGCCACCGGA+AGG 0.642394 1.3:-75507190 None:intergenic
CGAAGTTGAGGAGTCCGACA+CGG 0.647237 1.3:-75507143 None:intergenic
GGAACCACCACCCATGACGA+CGG 0.659927 1.3:-75507775 None:intergenic
TGTTGTTCTAAGCCAGAGCA+GGG 0.670625 1.3:-75507936 None:intergenic
ACCCGAGCAGATCCTTCCGG+TGG 0.672658 1.3:+75507178 MS.gene71830:CDS
AATTCAGGATGCTCGATTCG+GGG 0.678593 1.3:+75507729 MS.gene71830:CDS
AGTTGAGGAGTCCGACACGG+CGG 0.682882 1.3:-75507140 None:intergenic
TTGAGGAGTCCGACACGGCG+GGG 0.686362 1.3:-75507138 None:intergenic
GCGGGGCTTAGGGCAAACGA+CGG 0.692402 1.3:-75507121 None:intergenic
GGGTTAGCTACTCTGCTCGG+CGG 0.725325 1.3:-75507703 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTCATATAAAATAATAG+AGG - chr1.3:75507389-75507408 None:intergenic 10.0%
!!! CTATTATTTTATATGAATTT+AGG + chr1.3:75507389-75507408 MS.gene71830:intron 10.0%
!!! AATTGATCTATTTTTAACAT+TGG + chr1.3:75507467-75507486 MS.gene71830:intron 15.0%
!!! ATATGAATTTAGGTTAAAAT+TGG + chr1.3:75507399-75507418 MS.gene71830:intron 15.0%
!!! TTTTCTCTTTTAATTTGCTT+TGG + chr1.3:75507239-75507258 MS.gene71830:intron 20.0%
! AATAGAGGAATTATTTACCT+TGG - chr1.3:75507374-75507393 None:intergenic 25.0%
ACACAAAGCAATTCAACAAA+AGG - chr1.3:75507563-75507582 None:intergenic 30.0%
AATCGAGCATCCTGAATTAG+TGG - chr1.3:75507727-75507746 None:intergenic 40.0%
AGTAGCTAACCCACTAATTC+AGG + chr1.3:75507714-75507733 MS.gene71830:CDS 40.0%
ATCGAGCATCCTGAATTAGT+GGG - chr1.3:75507726-75507745 None:intergenic 40.0%
CTAATTCAGGATGCTCGATT+CGG + chr1.3:75507727-75507746 MS.gene71830:CDS 40.0%
GCAGATGAGTTTGAATCACA+CGG - chr1.3:75507325-75507344 None:intergenic 40.0%
GCAGGATGATGATTGTGATA+CGG + chr1.3:75507627-75507646 MS.gene71830:intron 40.0%
TAATTCAGGATGCTCGATTC+GGG + chr1.3:75507728-75507747 MS.gene71830:CDS 40.0%
TTGAATCACACGGTTCAACT+TGG - chr1.3:75507315-75507334 None:intergenic 40.0%
TTGGTGAGAATTGTGTCCAA+GGG - chr1.3:75507355-75507374 None:intergenic 40.0%
!! CAATTTTGGTAACAATCCGG+CGG + chr1.3:75507861-75507880 MS.gene71830:CDS 40.0%
!! GGCCAATTTTGGTAACAATC+CGG + chr1.3:75507858-75507877 MS.gene71830:CDS 40.0%
!! GTTGTGCTTTTGTGATTTGC+AGG + chr1.3:75507609-75507628 MS.gene71830:intron 40.0%
AAATTCACCTGAGATGCCAC+CGG - chr1.3:75507197-75507216 None:intergenic 45.0%
AAGGATTCGATTGCCTTGAC+AGG + chr1.3:75507893-75507912 MS.gene71830:CDS 45.0%
AGGATTCGATTGCCTTGACA+GGG + chr1.3:75507894-75507913 MS.gene71830:CDS 45.0%
CGCCGGATTGTTACCAAAAT+TGG - chr1.3:75507863-75507882 None:intergenic 45.0%
CTTGGACACAATTCTCACCA+AGG + chr1.3:75507354-75507373 MS.gene71830:intron 45.0%
CTTGGTGAGAATTGTGTCCA+AGG - chr1.3:75507356-75507375 None:intergenic 45.0%
! AATTCAGGATGCTCGATTCG+GGG + chr1.3:75507729-75507748 MS.gene71830:CDS 45.0%
AAGGAAGGGAGGATGTGTTC+GGG + chr1.3:75507837-75507856 MS.gene71830:CDS 50.0%
AATTACGGCTGTCCCTGTCA+AGG - chr1.3:75507909-75507928 None:intergenic 50.0%
CAAGGAAGGGAGGATGTGTT+CGG + chr1.3:75507836-75507855 MS.gene71830:CDS 50.0%
CGGAGACCCACAGAAAAATG+GGG - chr1.3:75507686-75507705 None:intergenic 50.0%
CTCATCTGCGTCAAATCCCT+TGG + chr1.3:75507336-75507355 MS.gene71830:intron 50.0%
CTCCCTTCCTTGCAGAAGAT+GGG - chr1.3:75507828-75507847 None:intergenic 50.0%
GAGCAGGGATGCTGCAATTA+CGG - chr1.3:75507924-75507943 None:intergenic 50.0%
GCGGAGACCCACAGAAAAAT+GGG - chr1.3:75507687-75507706 None:intergenic 50.0%
GGAGACCCACAGAAAAATGG+GGG - chr1.3:75507685-75507704 None:intergenic 50.0%
TAATTGCAGCATCCCTGCTC+TGG + chr1.3:75507924-75507943 MS.gene71830:CDS 50.0%
TCCCTTCCTTGCAGAAGATG+GGG - chr1.3:75507827-75507846 None:intergenic 50.0%
! GGATGTGTTCGGGCCAATTT+TGG + chr1.3:75507847-75507866 MS.gene71830:CDS 50.0%
!! AGTGGGTTAGCTACTCTGCT+CGG - chr1.3:75507709-75507728 None:intergenic 50.0%
AATCCTTCGACTCTCACCGC+CGG - chr1.3:75507880-75507899 None:intergenic 55.0%
ACTACCCGAGCAGATCCTTC+CGG + chr1.3:75507175-75507194 MS.gene71830:CDS 55.0%
AGTCGTTGACGGCGAAGTTG+AGG - chr1.3:75507158-75507177 None:intergenic 55.0%
CCATCTTCTGCAAGGAAGGG+AGG + chr1.3:75507826-75507845 MS.gene71830:CDS 55.0%
CCCCCATCTTCTGCAAGGAA+GGG + chr1.3:75507823-75507842 MS.gene71830:CDS 55.0%
CCCTTCCTTGCAGAAGATGG+GGG - chr1.3:75507826-75507845 None:intergenic 55.0%
CCTCCCTTCCTTGCAGAAGA+TGG - chr1.3:75507829-75507848 None:intergenic 55.0%
CGAAGTTGAGGAGTCCGACA+CGG - chr1.3:75507146-75507165 None:intergenic 55.0%
CTTCTCCCCCATCTTCTGCA+AGG + chr1.3:75507818-75507837 MS.gene71830:CDS 55.0%
CTTGCAGAAGATGGGGGAGA+AGG - chr1.3:75507820-75507839 None:intergenic 55.0%
GACCCACAGAAAAATGGGGG+CGG - chr1.3:75507682-75507701 None:intergenic 55.0%
GGCGGAGACCCACAGAAAAA+TGG - chr1.3:75507688-75507707 None:intergenic 55.0%
TCCCCCATCTTCTGCAAGGA+AGG + chr1.3:75507822-75507841 MS.gene71830:CDS 55.0%
TCTGCTCGGGTAGTCGTTGA+CGG - chr1.3:75507169-75507188 None:intergenic 55.0%
AATCCGGCGGTGAGAGTCGA+AGG + chr1.3:75507874-75507893 MS.gene71830:CDS 60.0%
ACCCATGACGACGGTGAGAG+GGG - chr1.3:75507769-75507788 None:intergenic 60.0%
AGAAGGTGGCAGACCGGATG+GGG - chr1.3:75507803-75507822 None:intergenic 60.0%
AGTTGAGGAGTCCGACACGG+CGG - chr1.3:75507143-75507162 None:intergenic 60.0%
CACCCATGACGACGGTGAGA+GGG - chr1.3:75507770-75507789 None:intergenic 60.0%
GAGAAGGTGGCAGACCGGAT+GGG - chr1.3:75507804-75507823 None:intergenic 60.0%
GATCCTTCCGGTGGCATCTC+AGG + chr1.3:75507187-75507206 MS.gene71830:CDS 60.0%
GCAGAAGATGGGGGAGAAGG+TGG - chr1.3:75507817-75507836 None:intergenic 60.0%
GGAACCACCACCCATGACGA+CGG - chr1.3:75507778-75507797 None:intergenic 60.0%
TCACCTGAGATGCCACCGGA+AGG - chr1.3:75507193-75507212 None:intergenic 60.0%
TGCCACCGGAAGGATCTGCT+CGG - chr1.3:75507183-75507202 None:intergenic 60.0%
!! CGCCGCCCCCATTTTTCTGT+GGG + chr1.3:75507677-75507696 MS.gene71830:CDS 60.0%
!! GGGTTAGCTACTCTGCTCGG+CGG - chr1.3:75507706-75507725 None:intergenic 60.0%
!! TCGCCGCCCCCATTTTTCTG+TGG + chr1.3:75507676-75507695 MS.gene71830:CDS 60.0%
ACCCGAGCAGATCCTTCCGG+TGG + chr1.3:75507178-75507197 MS.gene71830:CDS 65.0%
CCACCCATGACGACGGTGAG+AGG - chr1.3:75507771-75507790 None:intergenic 65.0%
CCTCTCACCGTCGTCATGGG+TGG + chr1.3:75507768-75507787 MS.gene71830:CDS 65.0%
CTCACCGTCGTCATGGGTGG+TGG + chr1.3:75507771-75507790 MS.gene71830:CDS 65.0%
GAAAAATGGGGGCGGCGACG+AGG - chr1.3:75507674-75507693 None:intergenic 65.0%
GCCACCGGAAGGATCTGCTC+GGG - chr1.3:75507182-75507201 None:intergenic 65.0%
GCCCCTCTCACCGTCGTCAT+GGG + chr1.3:75507765-75507784 MS.gene71830:CDS 65.0%
GCGGGGCTTAGGGCAAACGA+CGG - chr1.3:75507124-75507143 None:intergenic 65.0%
GGAGAAGGTGGCAGACCGGA+TGG - chr1.3:75507805-75507824 None:intergenic 65.0%
GTGGTGGTTCCTGCCCCATC+CGG + chr1.3:75507787-75507806 MS.gene71830:CDS 65.0%
GTTGAGGAGTCCGACACGGC+GGG - chr1.3:75507142-75507161 None:intergenic 65.0%
TGGGGGAGAAGGTGGCAGAC+CGG - chr1.3:75507809-75507828 None:intergenic 65.0%
TTGAGGAGTCCGACACGGCG+GGG - chr1.3:75507141-75507160 None:intergenic 65.0%
AGTCCGACACGGCGGGGCTT+AGG - chr1.3:75507135-75507154 None:intergenic 70.0%
CGCCCCTCTCACCGTCGTCA+TGG + chr1.3:75507764-75507783 MS.gene71830:CDS 70.0%
GTCCGACACGGCGGGGCTTA+GGG - chr1.3:75507134-75507153 None:intergenic 70.0%
TGCCCTAAGCCCCGCCGTGT+CGG + chr1.3:75507129-75507148 MS.gene71830:CDS 70.0%
GGCGGCGACGAGGCTACTGG+AGG - chr1.3:75507664-75507683 None:intergenic 75.0%
GGTGGCAGACCGGATGGGGC+AGG - chr1.3:75507799-75507818 None:intergenic 75.0%
! GGGGGCGGCGACGAGGCTAC+TGG - chr1.3:75507667-75507686 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 75507105 75507951 75507105 ID=MS.gene71830
chr1.3 mRNA 75507105 75507951 75507105 ID=MS.gene71830.t1;Parent=MS.gene71830
chr1.3 exon 75507105 75507208 75507105 ID=MS.gene71830.t1.exon1;Parent=MS.gene71830.t1
chr1.3 CDS 75507105 75507208 75507105 ID=cds.MS.gene71830.t1;Parent=MS.gene71830.t1
chr1.3 exon 75507660 75507951 75507660 ID=MS.gene71830.t1.exon2;Parent=MS.gene71830.t1
chr1.3 CDS 75507660 75507951 75507660 ID=cds.MS.gene71830.t1;Parent=MS.gene71830.t1
Gene Sequence

>MS.gene71830

ATGAACGGAAGAGACTCCGTCGTTTGCCCTAAGCCCCGCCGTGTCGGACTCCTCAACTTCGCCGTCAACGACTACCCGAGCAGATCCTTCCGGTGGCATCTCAGGTGAATTTTAATTTCTCTCTGTTTTTCTTCTTTTCTCTTTTAATTTGCTTTGGTTCAATTTTATTAAACTTTTGCATTTTTCGTTTTTCTTCTTCTGCAGTTGCCAAGTTGAACCGTGTGATTCAAACTCATCTGCGTCAAATCCCTTGGACACAATTCTCACCAAGGTAAATAATTCCTCTATTATTTTATATGAATTTAGGTTAAAATTGGAATAATTTATCTATTATTTTATATGAATTTAGGTTAAATTTTAATAATTGATCTATTTTTAACATTGGTTAACGTTAACTTGTTACTTGTTAAAATTTGAATAGTTGATTGATTACAGTTAAAGCTTGAATTATTGTGCCTTTTGTTGAATTGCTTTGTGTTATGCTTTTTGATTGAATTGTTCTGTGTTGTGCTTTTGTGATTTGCAGGATGATGATTGTGATACGGAACAATCAAGTCCTCCAGTAGCCTCGTCGCCGCCCCCATTTTTCTGTGGGTCTCCGCCGAGCAGAGTAGCTAACCCACTAATTCAGGATGCTCGATTCGGGGATGAGAATTTCTCGCCCCTCTCACCGTCGTCATGGGTGGTGGTTCCTGCCCCATCCGGTCTGCCACCTTCTCCCCCATCTTCTGCAAGGAAGGGAGGATGTGTTCGGGCCAATTTTGGTAACAATCCGGCGGTGAGAGTCGAAGGATTCGATTGCCTTGACAGGGACAGCCGTAATTGCAGCATCCCTGCTCTGGCTTAG

Protein sequence

>MS.gene71830.t1

MNGRDSVVCPKPRRVGLLNFAVNDYPSRSFRWHLSPPVASSPPPFFCGSPPSRVANPLIQDARFGDENFSPLSPSSWVVVPAPSGLPPSPPSSARKGGCVRANFGNNPAVRVEGFDCLDRDSRNCSIPALA