AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72178


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72178.t1 AT5G22250 64.662 266 90 3 9 270 12 277 6.40e-120 344
MS.gene72178.t1 AT3G44260 61.132 265 98 3 9 270 17 279 4.51e-116 335
MS.gene72178.t1 AT2G32070 50.746 268 127 5 4 270 5 268 4.63e-92 273
MS.gene72178.t1 AT5G10960 50.562 267 128 4 4 270 5 267 7.14e-91 270
MS.gene72178.t1 AT1G80780 49.064 267 132 4 4 270 5 267 1.27e-88 265
MS.gene72178.t1 AT1G80780 49.064 267 132 4 4 270 5 267 1.27e-88 265
MS.gene72178.t1 AT1G80780 49.064 267 132 4 4 270 5 267 1.73e-88 265
MS.gene72178.t1 AT1G15920 47.407 270 134 5 5 269 10 276 8.64e-81 245
MS.gene72178.t1 AT1G15920 47.407 270 134 5 5 269 10 276 8.64e-81 245
MS.gene72178.t1 AT1G61470 37.037 243 130 7 13 249 4 229 1.14e-38 137
MS.gene72178.t1 AT1G06450 33.700 273 157 9 1 268 1 254 1.51e-38 138
MS.gene72178.t1 AT1G27820 35.510 245 143 5 7 248 2 234 8.44e-34 125
MS.gene72178.t1 AT1G27890 35.565 239 139 5 13 248 3 229 1.60e-32 121
MS.gene72178.t1 AT3G44240 35.242 227 132 7 25 249 3 216 3.77e-32 119

Find 62 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCACAGCGCTTATGATTT+TGG 0.104590 4.1:+6060696 MS.gene72178:CDS
ATCAAATTCAACCTCTAAAT+TGG 0.207989 4.1:-6060265 None:intergenic
GATGGCAACCTTCCTCATTT+TGG 0.210731 4.1:+6060468 MS.gene72178:CDS
AAACACGCTGGGGTACTATT+TGG 0.216392 4.1:+6060993 MS.gene72178:CDS
GCTGAATTGATGCTCACTTC+TGG 0.258268 4.1:+6060639 MS.gene72178:CDS
CAATCATCATCCGTCAAGTT+TGG 0.286714 4.1:+6060238 MS.gene72178:CDS
TCTTCAACAAATCAGTTGTT+TGG 0.295934 4.1:+6060667 MS.gene72178:CDS
AACGGTTCGGCAGATTCCTT+CGG 0.352418 4.1:-6060740 None:intergenic
CTTAACGTGGGTCGGGTTGC+TGG 0.360050 4.1:+6060879 MS.gene72178:CDS
AGATGACGGCGGTCAACTTT+GGG 0.368577 4.1:-6060360 None:intergenic
CTTCAACAAATCAGTTGTTT+GGG 0.376564 4.1:+6060668 MS.gene72178:CDS
GGACAAATAGCCGCTACATA+TGG 0.395638 4.1:+6060490 MS.gene72178:CDS
ACGGTTCGGCAGATTCCTTC+GGG 0.395658 4.1:-6060739 None:intergenic
AATCATCATCCGTCAAGTTT+GGG 0.414340 4.1:+6060239 MS.gene72178:CDS
AAGATGACGGCGGTCAACTT+TGG 0.418710 4.1:-6060361 None:intergenic
GAATCTGCCGAACCGTTTAG+AGG 0.420121 4.1:+6060746 MS.gene72178:CDS
AGTTTGGGCATCCAATTTAG+AGG 0.424325 4.1:+6060254 MS.gene72178:CDS
TCCATGGATACTGAATTTCC+AGG 0.437255 4.1:+6060324 MS.gene72178:CDS
GGTTGCCATCACCATCAGAT+AGG 0.449581 4.1:-6060455 None:intergenic
CAAACTTGACGGATGATGAT+TGG 0.450788 4.1:-6060237 None:intergenic
TACTCATTTCTTGAATAATG+AGG 0.477666 4.1:+6060965 MS.gene72178:CDS
TGACCACTATCGTTACTTGA+AGG 0.492905 4.1:+6060392 MS.gene72178:CDS
GACAAATAGCCGCTACATAT+GGG 0.500452 4.1:+6060491 MS.gene72178:CDS
CAATTGAGCCTTGGTTGTAA+AGG 0.504025 4.1:-6060548 None:intergenic
TGCGCTTGAAGTCAATCCCT+TGG 0.505546 4.1:-6060584 None:intergenic
TCAATATCCTTTCATCTCCA+TGG 0.506697 4.1:+6060308 MS.gene72178:CDS
TACAACACTTAACGTGGGTC+GGG 0.508105 4.1:+6060872 MS.gene72178:CDS
AATTGGATGCCCAAACTTGA+CGG 0.510297 4.1:-6060248 None:intergenic
ACCTGGAAATTCAGTATCCA+TGG 0.514112 4.1:-6060325 None:intergenic
AGGTTCTGCAACGCTTTGTA+CGG 0.515634 4.1:+6060831 MS.gene72178:CDS
TTCAAGCGCAATTTATCTCA+TGG 0.520248 4.1:+6060597 MS.gene72178:CDS
CAGATAGGGTGATACCAACT+TGG 0.523809 4.1:-6060440 None:intergenic
GATATTGATTGACAACTTGA+CGG 0.523978 4.1:-6060293 None:intergenic
GCATGCATTTAAGAAAATGA+TGG 0.528476 4.1:+6060941 MS.gene72178:CDS
CTACAACACTTAACGTGGGT+CGG 0.533742 4.1:+6060871 MS.gene72178:CDS
CGGCTATTTGTCCCAAAATG+AGG 0.540450 4.1:-6060480 None:intergenic
GCTGGAAAGTCTCATCAAGC+TGG 0.542113 4.1:+6060897 MS.gene72178:CDS
ATTGACATGCTCTGCCGCCA+AGG 0.544877 4.1:+6060567 MS.gene72178:CDS
AGAGCATGTCAATTGAGCCT+TGG 0.548111 4.1:-6060557 None:intergenic
TTGACATGCTCTGCCGCCAA+GGG 0.557345 4.1:+6060568 MS.gene72178:CDS
GCTTGAAGTCAATCCCTTGG+CGG 0.562753 4.1:-6060581 None:intergenic
GCTCTTAAACTCATCCAAGT+TGG 0.568904 4.1:+6060426 MS.gene72178:CDS
ATGATATGAAACACATGATG+AGG 0.569362 4.1:+6060811 MS.gene72178:CDS
TTGGGTGAATAAATTACACC+TGG 0.570783 4.1:-6060342 None:intergenic
GTTGCCATCACCATCAGATA+GGG 0.583084 4.1:-6060454 None:intergenic
TTCTGCAACGCTTTGTACGG+TGG 0.584961 4.1:+6060834 MS.gene72178:CDS
ATTCAAAATGAACCAGCTGA+AGG 0.587598 4.1:+6060209 None:intergenic
GGTCAGAGGGTTTAAGATGA+CGG 0.592880 4.1:-6060374 None:intergenic
AAGTAACGATAGTGGTCAGA+GGG 0.593594 4.1:-6060387 None:intergenic
TAGTGAAGATATTGACCCGA+AGG 0.595020 4.1:+6060724 MS.gene72178:CDS
TCAGTATCCATGGAGATGAA+AGG 0.598752 4.1:-6060315 None:intergenic
GTTGGTATCACCCTATCTGA+TGG 0.609828 4.1:+6060444 MS.gene72178:CDS
TGACCTTCAAGTAACGATAG+TGG 0.622378 4.1:-6060395 None:intergenic
TATTTGTCCCAAAATGAGGA+AGG 0.623706 4.1:-6060476 None:intergenic
AGTCGCTACAACACTTAACG+TGG 0.625698 4.1:+6060866 MS.gene72178:CDS
CAAGTAACGATAGTGGTCAG+AGG 0.631701 4.1:-6060388 None:intergenic
GAGCATGACCTTTACAACCA+AGG 0.646186 4.1:+6060540 MS.gene72178:CDS
AGTTGAATTCCCATATGTAG+CGG 0.649610 4.1:-6060500 None:intergenic
GATCAGATAGTTTGTTGACG+TGG 0.660381 4.1:+6060919 MS.gene72178:CDS
ATCACCCTATCTGATGGTGA+TGG 0.683961 4.1:+6060450 MS.gene72178:CDS
GTCGCTACAACACTTAACGT+GGG 0.701411 4.1:+6060867 MS.gene72178:CDS
CAGAGGGTTTAAGATGACGG+CGG 0.736962 4.1:-6060371 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTAAAAAGTCCTCTAAA+CGG - chr4.1:6060761-6060780 None:intergenic 20.0%
! ATCAAATTCAACCTCTAAAT+TGG - chr4.1:6060268-6060287 None:intergenic 25.0%
! TACTCATTTCTTGAATAATG+AGG + chr4.1:6060965-6060984 MS.gene72178:CDS 25.0%
ATGATATGAAACACATGATG+AGG + chr4.1:6060811-6060830 MS.gene72178:CDS 30.0%
CTTCAACAAATCAGTTGTTT+GGG + chr4.1:6060668-6060687 MS.gene72178:CDS 30.0%
GCATGCATTTAAGAAAATGA+TGG + chr4.1:6060941-6060960 MS.gene72178:CDS 30.0%
TCTTCAACAAATCAGTTGTT+TGG + chr4.1:6060667-6060686 MS.gene72178:CDS 30.0%
! GATATTGATTGACAACTTGA+CGG - chr4.1:6060296-6060315 None:intergenic 30.0%
AAAAAGTCCTCTAAACGGTT+CGG - chr4.1:6060756-6060775 None:intergenic 35.0%
AATCATCATCCGTCAAGTTT+GGG + chr4.1:6060239-6060258 MS.gene72178:CDS 35.0%
AGTTGAATTCCCATATGTAG+CGG - chr4.1:6060503-6060522 None:intergenic 35.0%
TATTTGTCCCAAAATGAGGA+AGG - chr4.1:6060479-6060498 None:intergenic 35.0%
TCAATATCCTTTCATCTCCA+TGG + chr4.1:6060308-6060327 MS.gene72178:CDS 35.0%
TTCAAGCGCAATTTATCTCA+TGG + chr4.1:6060597-6060616 MS.gene72178:CDS 35.0%
TTGGGTGAATAAATTACACC+TGG - chr4.1:6060345-6060364 None:intergenic 35.0%
! TTCACAGCGCTTATGATTTT+GGG + chr4.1:6060697-6060716 MS.gene72178:CDS 35.0%
! TTTCACAGCGCTTATGATTT+TGG + chr4.1:6060696-6060715 MS.gene72178:CDS 35.0%
AATTGGATGCCCAAACTTGA+CGG - chr4.1:6060251-6060270 None:intergenic 40.0%
ACCTGGAAATTCAGTATCCA+TGG - chr4.1:6060328-6060347 None:intergenic 40.0%
AGTTTGGGCATCCAATTTAG+AGG + chr4.1:6060254-6060273 MS.gene72178:CDS 40.0%
CAATCATCATCCGTCAAGTT+TGG + chr4.1:6060238-6060257 MS.gene72178:CDS 40.0%
CAATTGAGCCTTGGTTGTAA+AGG - chr4.1:6060551-6060570 None:intergenic 40.0%
GACAAATAGCCGCTACATAT+GGG + chr4.1:6060491-6060510 MS.gene72178:CDS 40.0%
GCTCTTAAACTCATCCAAGT+TGG + chr4.1:6060426-6060445 MS.gene72178:CDS 40.0%
TAGTGAAGATATTGACCCGA+AGG + chr4.1:6060724-6060743 MS.gene72178:CDS 40.0%
TCAGTATCCATGGAGATGAA+AGG - chr4.1:6060318-6060337 None:intergenic 40.0%
TCCATGGATACTGAATTTCC+AGG + chr4.1:6060324-6060343 MS.gene72178:CDS 40.0%
TGACCACTATCGTTACTTGA+AGG + chr4.1:6060392-6060411 MS.gene72178:CDS 40.0%
TGACCTTCAAGTAACGATAG+TGG - chr4.1:6060398-6060417 None:intergenic 40.0%
! AAGTAACGATAGTGGTCAGA+GGG - chr4.1:6060390-6060409 None:intergenic 40.0%
! ATGGCAACCTTCCTCATTTT+GGG + chr4.1:6060469-6060488 MS.gene72178:CDS 40.0%
! CAAACTTGACGGATGATGAT+TGG - chr4.1:6060240-6060259 None:intergenic 40.0%
!! GATCAGATAGTTTGTTGACG+TGG + chr4.1:6060919-6060938 MS.gene72178:CDS 40.0%
AATGAGGCTCAAAAACACGC+TGG + chr4.1:6060981-6061000 MS.gene72178:CDS 45.0%
AGAGCATGTCAATTGAGCCT+TGG - chr4.1:6060560-6060579 None:intergenic 45.0%
AGGTTCTGCAACGCTTTGTA+CGG + chr4.1:6060831-6060850 MS.gene72178:CDS 45.0%
AGTCGCTACAACACTTAACG+TGG + chr4.1:6060866-6060885 MS.gene72178:CDS 45.0%
ATGAGGCTCAAAAACACGCT+GGG + chr4.1:6060982-6061001 MS.gene72178:CDS 45.0%
CAGATAGGGTGATACCAACT+TGG - chr4.1:6060443-6060462 None:intergenic 45.0%
CGGCTATTTGTCCCAAAATG+AGG - chr4.1:6060483-6060502 None:intergenic 45.0%
CTACAACACTTAACGTGGGT+CGG + chr4.1:6060871-6060890 MS.gene72178:CDS 45.0%
GAGCATGACCTTTACAACCA+AGG + chr4.1:6060540-6060559 MS.gene72178:CDS 45.0%
GCTGAATTGATGCTCACTTC+TGG + chr4.1:6060639-6060658 MS.gene72178:CDS 45.0%
GGACAAATAGCCGCTACATA+TGG + chr4.1:6060490-6060509 MS.gene72178:CDS 45.0%
GTCGCTACAACACTTAACGT+GGG + chr4.1:6060867-6060886 MS.gene72178:CDS 45.0%
GTTGCCATCACCATCAGATA+GGG - chr4.1:6060457-6060476 None:intergenic 45.0%
GTTGGTATCACCCTATCTGA+TGG + chr4.1:6060444-6060463 MS.gene72178:CDS 45.0%
TACAACACTTAACGTGGGTC+GGG + chr4.1:6060872-6060891 MS.gene72178:CDS 45.0%
! AAACACGCTGGGGTACTATT+TGG + chr4.1:6060993-6061012 MS.gene72178:CDS 45.0%
! ATCACCCTATCTGATGGTGA+TGG + chr4.1:6060450-6060469 MS.gene72178:CDS 45.0%
! CAAGTAACGATAGTGGTCAG+AGG - chr4.1:6060391-6060410 None:intergenic 45.0%
! GATGGCAACCTTCCTCATTT+TGG + chr4.1:6060468-6060487 MS.gene72178:CDS 45.0%
! GGTCAGAGGGTTTAAGATGA+CGG - chr4.1:6060377-6060396 None:intergenic 45.0%
!!! TGATTGGCTTTTCCTTCAGC+TGG - chr4.1:6060224-6060243 None:intergenic 45.0%
!!! AATATTTTAACAATATTATT+TGG + chr4.1:6060777-6060796 MS.gene72178:CDS 5.0%
GAATCTGCCGAACCGTTTAG+AGG + chr4.1:6060746-6060765 MS.gene72178:CDS 50.0%
GCTGGAAAGTCTCATCAAGC+TGG + chr4.1:6060897-6060916 MS.gene72178:CDS 50.0%
GCTTGAAGTCAATCCCTTGG+CGG - chr4.1:6060584-6060603 None:intergenic 50.0%
GGTTGCCATCACCATCAGAT+AGG - chr4.1:6060458-6060477 None:intergenic 50.0%
TGAGGCTCAAAAACACGCTG+GGG + chr4.1:6060983-6061002 MS.gene72178:CDS 50.0%
TGCGCTTGAAGTCAATCCCT+TGG - chr4.1:6060587-6060606 None:intergenic 50.0%
TTCTGCAACGCTTTGTACGG+TGG + chr4.1:6060834-6060853 MS.gene72178:CDS 50.0%
! AACGGTTCGGCAGATTCCTT+CGG - chr4.1:6060743-6060762 None:intergenic 50.0%
! AAGATGACGGCGGTCAACTT+TGG - chr4.1:6060364-6060383 None:intergenic 50.0%
! AGATGACGGCGGTCAACTTT+GGG - chr4.1:6060363-6060382 None:intergenic 50.0%
! CAGAGGGTTTAAGATGACGG+CGG - chr4.1:6060374-6060393 None:intergenic 50.0%
ATTGACATGCTCTGCCGCCA+AGG + chr4.1:6060567-6060586 MS.gene72178:CDS 55.0%
TTGACATGCTCTGCCGCCAA+GGG + chr4.1:6060568-6060587 MS.gene72178:CDS 55.0%
! ACGGTTCGGCAGATTCCTTC+GGG - chr4.1:6060742-6060761 None:intergenic 55.0%
! CTTAACGTGGGTCGGGTTGC+TGG + chr4.1:6060879-6060898 MS.gene72178:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 6060216 6061034 6060216 ID=MS.gene72178
chr4.1 mRNA 6060216 6061034 6060216 ID=MS.gene72178.t1;Parent=MS.gene72178
chr4.1 exon 6060216 6061034 6060216 ID=MS.gene72178.t1.exon1;Parent=MS.gene72178.t1
chr4.1 CDS 6060216 6061034 6060216 ID=cds.MS.gene72178.t1;Parent=MS.gene72178.t1
Gene Sequence

>MS.gene72178

ATGAACCAGCTGAAGGAAAAGCCAATCATCATCCGTCAAGTTTGGGCATCCAATTTAGAGGTTGAATTTGATCTCATCCGTCAAGTTGTCAATCAATATCCTTTCATCTCCATGGATACTGAATTTCCAGGTGTAATTTATTCACCCAAAGTTGACCGCCGTCATCTTAAACCCTCTGACCACTATCGTTACTTGAAGGTCAACGTTGATGCTCTTAAACTCATCCAAGTTGGTATCACCCTATCTGATGGTGATGGCAACCTTCCTCATTTTGGGACAAATAGCCGCTACATATGGGAATTCAACTTTTGCGATTTTGATTTTGAGCATGACCTTTACAACCAAGGCTCAATTGACATGCTCTGCCGCCAAGGGATTGACTTCAAGCGCAATTTATCTCATGGTGTGAATTCGTCACGTTTTGCTGAATTGATGCTCACTTCTGGACTTGTCTTCAACAAATCAGTTGTTTGGGTCACATTTCACAGCGCTTATGATTTTGGGTATTTAGTGAAGATATTGACCCGAAGGAATCTGCCGAACCGTTTAGAGGACTTTTTAAATATTTTAACAATATTATTTGGAAAGAATGTTTATGATATGAAACACATGATGAGGTTCTGCAACGCTTTGTACGGTGGTCTCGAGCGAGTCGCTACAACACTTAACGTGGGTCGGGTTGCTGGAAAGTCTCATCAAGCTGGATCAGATAGTTTGTTGACGTGGCATGCATTTAAGAAAATGATGGATACTCATTTCTTGAATAATGAGGCTCAAAAACACGCTGGGGTACTATTTGGATTAGAAATTGCAACTTAG

Protein sequence

>MS.gene72178.t1

MNQLKEKPIIIRQVWASNLEVEFDLIRQVVNQYPFISMDTEFPGVIYSPKVDRRHLKPSDHYRYLKVNVDALKLIQVGITLSDGDGNLPHFGTNSRYIWEFNFCDFDFEHDLYNQGSIDMLCRQGIDFKRNLSHGVNSSRFAELMLTSGLVFNKSVVWVTFHSAYDFGYLVKILTRRNLPNRLEDFLNILTILFGKNVYDMKHMMRFCNALYGGLERVATTLNVGRVAGKSHQAGSDSLLTWHAFKKMMDTHFLNNEAQKHAGVLFGLEIAT