Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75621.t1 | XP_003601765.1 | 99 | 414 | 4 | 0 | 4 | 417 | 52 | 465 | 1.20E-237 | 832 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75621.t1 | Q9FZK1 | 67.1 | 416 | 135 | 2 | 4 | 417 | 52 | 467 | 7.5e-172 | 604.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75621.t1 | G7JBG1 | 99.0 | 414 | 4 | 0 | 4 | 417 | 52 | 465 | 8.7e-238 | 832.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049195 | MS.gene75621 | -0.832685 | 8.21E-56 | -1.69E-46 |
| MS.gene04933 | MS.gene75621 | -0.803954 | 2.72E-49 | -1.69E-46 |
| MS.gene049351 | MS.gene75621 | -0.815714 | 8.02E-52 | -1.69E-46 |
| MS.gene049681 | MS.gene75621 | -0.804607 | 1.99E-49 | -1.69E-46 |
| MS.gene049993 | MS.gene75621 | -0.818451 | 1.95E-52 | -1.69E-46 |
| MS.gene050397 | MS.gene75621 | -0.831958 | 1.24E-55 | -1.69E-46 |
| MS.gene050650 | MS.gene75621 | -0.808511 | 2.99E-50 | -1.69E-46 |
| MS.gene050787 | MS.gene75621 | -0.803093 | 4.11E-49 | -1.69E-46 |
| MS.gene051525 | MS.gene75621 | -0.85082 | 1.33E-60 | -1.69E-46 |
| MS.gene051582 | MS.gene75621 | -0.820488 | 6.68E-53 | -1.69E-46 |
| MS.gene051860 | MS.gene75621 | -0.826275 | 2.97E-54 | -1.69E-46 |
| MS.gene052011 | MS.gene75621 | 0.873544 | 1.35E-67 | -1.69E-46 |
| MS.gene052253 | MS.gene75621 | -0.8296 | 4.70E-55 | -1.69E-46 |
| MS.gene053836 | MS.gene75621 | -0.846054 | 2.76E-59 | -1.69E-46 |
| MS.gene054011 | MS.gene75621 | -0.823033 | 1.72E-53 | -1.69E-46 |
| MS.gene054012 | MS.gene75621 | -0.818451 | 1.95E-52 | -1.69E-46 |
| MS.gene054013 | MS.gene75621 | -0.814711 | 1.34E-51 | -1.69E-46 |
| MS.gene054183 | MS.gene75621 | -0.828141 | 1.06E-54 | -1.69E-46 |
| MS.gene054473 | MS.gene75621 | -0.806336 | 8.64E-50 | -1.69E-46 |
| MS.gene055217 | MS.gene75621 | -0.81309 | 3.05E-51 | -1.69E-46 |
| MS.gene055277 | MS.gene75621 | -0.822258 | 2.61E-53 | -1.69E-46 |
| MS.gene055428 | MS.gene75621 | -0.824111 | 9.63E-54 | -1.69E-46 |
| MS.gene05553 | MS.gene75621 | -0.810375 | 1.19E-50 | -1.69E-46 |
| MS.gene056191 | MS.gene75621 | -0.813849 | 2.08E-51 | -1.69E-46 |
| MS.gene05702 | MS.gene75621 | -0.803313 | 3.70E-49 | -1.69E-46 |
| MS.gene057121 | MS.gene75621 | -0.801454 | 8.94E-49 | -1.69E-46 |
| MS.gene057476 | MS.gene75621 | -0.826447 | 2.70E-54 | -1.69E-46 |
| MS.gene05811 | MS.gene75621 | -0.81672 | 4.78E-52 | -1.69E-46 |
| MS.gene058233 | MS.gene75621 | -0.81926 | 1.28E-52 | -1.69E-46 |
| MS.gene05907 | MS.gene75621 | -0.836132 | 1.12E-56 | -1.69E-46 |
| MS.gene059420 | MS.gene75621 | -0.829687 | 4.48E-55 | -1.69E-46 |
| MS.gene05984 | MS.gene75621 | -0.807702 | 4.44E-50 | -1.69E-46 |
| MS.gene061049 | MS.gene75621 | -0.818538 | 1.86E-52 | -1.69E-46 |
| MS.gene061114 | MS.gene75621 | 0.805957 | 1.04E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75621.t1 | MTR_3g085160 | 99.275 | 414 | 3 | 0 | 4 | 417 | 52 | 465 | 0.0 | 863 |
| MS.gene75621.t1 | MTR_3g085160 | 99.242 | 396 | 3 | 0 | 22 | 417 | 1 | 396 | 0.0 | 821 |
| MS.gene75621.t1 | MTR_1g033280 | 46.950 | 377 | 165 | 10 | 57 | 416 | 18 | 376 | 1.87e-107 | 322 |
| MS.gene75621.t1 | MTR_1g033310 | 48.370 | 368 | 167 | 11 | 52 | 416 | 2 | 349 | 2.23e-107 | 321 |
| MS.gene75621.t1 | MTR_7g017400 | 30.939 | 362 | 224 | 15 | 43 | 391 | 68 | 416 | 1.91e-34 | 133 |
| MS.gene75621.t1 | MTR_1g014700 | 25.515 | 388 | 240 | 13 | 70 | 415 | 21 | 401 | 1.33e-30 | 122 |
| MS.gene75621.t1 | MTR_4g094748 | 25.707 | 389 | 238 | 12 | 63 | 415 | 55 | 428 | 1.43e-25 | 108 |
| MS.gene75621.t1 | MTR_8g028250 | 26.146 | 371 | 230 | 14 | 72 | 413 | 45 | 400 | 3.98e-23 | 100 |
| MS.gene75621.t1 | MTR_4g011090 | 25.648 | 386 | 234 | 16 | 67 | 415 | 1 | 370 | 2.19e-22 | 98.2 |
| MS.gene75621.t1 | MTR_8g028220 | 24.932 | 369 | 234 | 13 | 71 | 413 | 44 | 395 | 9.46e-20 | 90.9 |
| MS.gene75621.t1 | MTR_1g018140 | 23.204 | 362 | 227 | 12 | 63 | 392 | 16 | 358 | 2.17e-13 | 71.6 |
| MS.gene75621.t1 | MTR_2g033410 | 22.264 | 265 | 179 | 9 | 71 | 313 | 9 | 268 | 8.01e-12 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75621.t1 | AT1G27340 | 68.932 | 412 | 124 | 4 | 9 | 417 | 57 | 467 | 0.0 | 605 |
| MS.gene75621.t1 | AT5G15710 | 29.708 | 377 | 229 | 13 | 39 | 394 | 68 | 429 | 2.43e-32 | 127 |
| MS.gene75621.t1 | AT5G15710 | 29.708 | 377 | 229 | 13 | 39 | 394 | 68 | 429 | 2.43e-32 | 127 |
| MS.gene75621.t1 | AT1G30950 | 26.446 | 363 | 225 | 10 | 63 | 392 | 42 | 395 | 2.49e-31 | 124 |
| MS.gene75621.t1 | AT4G33160 | 23.148 | 432 | 251 | 14 | 51 | 417 | 42 | 457 | 7.47e-25 | 106 |
| MS.gene75621.t1 | AT4G33160 | 23.059 | 425 | 260 | 13 | 51 | 417 | 103 | 518 | 1.86e-24 | 105 |
| MS.gene75621.t1 | AT5G43190 | 25.833 | 360 | 224 | 13 | 58 | 392 | 38 | 379 | 2.73e-18 | 86.7 |
| MS.gene75621.t1 | AT3G61590 | 36.364 | 121 | 73 | 3 | 71 | 189 | 51 | 169 | 6.70e-17 | 82.4 |
| MS.gene75621.t1 | AT3G61590 | 36.364 | 121 | 73 | 3 | 71 | 189 | 51 | 169 | 6.70e-17 | 82.4 |
| MS.gene75621.t1 | AT3G61590 | 36.364 | 121 | 73 | 3 | 71 | 189 | 43 | 161 | 7.63e-17 | 82.4 |
| MS.gene75621.t1 | AT3G61590 | 36.364 | 121 | 73 | 3 | 71 | 189 | 79 | 197 | 9.02e-17 | 82.4 |
Find 119 sgRNAs with CRISPR-Local
Find 147 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGTCCAAGCACCATATATT+TGG | 0.306854 | 3.3:+71163175 | None:intergenic |
| ATACTTCCAGGCATGTTTCC+TGG | 0.307731 | 3.3:+71163481 | None:intergenic |
| TCATAGGAAACAACCCCTTC+TGG | 0.328091 | 3.3:+71163382 | None:intergenic |
| AAAGAATGGCTGAAGGTTCC+TGG | 0.339162 | 3.3:-71163046 | MS.gene75621:CDS |
| AGGGGTTGTTTCCTATGATA+TGG | 0.339167 | 3.3:-71163377 | MS.gene75621:CDS |
| ACCCATGAAAATGTGAATTC+AGG | 0.349764 | 3.3:-71163823 | MS.gene75621:CDS |
| GTTTCCTATGATATGGCAAC+AGG | 0.361646 | 3.3:-71163370 | MS.gene75621:CDS |
| AAGTTATGGAACAAGAAATA+TGG | 0.365064 | 3.3:-71164014 | MS.gene75621:CDS |
| TTCCATAACTTCACTTGTAC+TGG | 0.365202 | 3.3:+71164026 | None:intergenic |
| CCGTCAATGGAATTCCTTGC+TGG | 0.368398 | 3.3:-71163911 | MS.gene75621:CDS |
| CACTGCCGTTTCTTCCCATT+CGG | 0.369756 | 3.3:+71163013 | None:intergenic |
| CCCCTGCTTCGATCTCTTCG+AGG | 0.376451 | 3.3:+71164066 | None:intergenic |
| GATTCATTTGTTTCGATCTC+AGG | 0.380457 | 3.3:+71163095 | None:intergenic |
| TAGACAGAATGCCAAATATA+TGG | 0.389573 | 3.3:-71163186 | MS.gene75621:CDS |
| TGCCATTGTCCCTTAACTTC+AGG | 0.389987 | 3.3:-71163453 | MS.gene75621:CDS |
| ACAGCCTCAAATAAATCTTC+AGG | 0.391468 | 3.3:+71163982 | None:intergenic |
| ATGATATGGCAACAGGGGTT+TGG | 0.394545 | 3.3:-71163363 | MS.gene75621:CDS |
| CCCACTAACTCAATCCTTCA+AGG | 0.400080 | 3.3:-71163656 | MS.gene75621:CDS |
| CAGTTTGCGACCTGAAGTTA+AGG | 0.407146 | 3.3:+71163443 | None:intergenic |
| CGAAGCAGGGGCAAATCATC+AGG | 0.419820 | 3.3:-71164054 | MS.gene75621:CDS |
| AATGGCAGGTTCATACTTCC+AGG | 0.420328 | 3.3:+71163469 | None:intergenic |
| AATGGAAATTCAATCAGTTC+AGG | 0.423765 | 3.3:-71163577 | MS.gene75621:CDS |
| ATCCAGTACAAGTGAAGTTA+TGG | 0.433150 | 3.3:-71164028 | MS.gene75621:CDS |
| TCCTTGAAGGATTGAGTTAG+TGG | 0.433327 | 3.3:+71163655 | None:intergenic |
| CCGAGCTGGCAACTCCTTGA+AGG | 0.435297 | 3.3:+71163642 | None:intergenic |
| CAGCCACGTGTGTATGCATA+TGG | 0.440067 | 3.3:-71163243 | MS.gene75621:CDS |
| ATCTGAACGCATGAAGTATA+GGG | 0.440759 | 3.3:+71163405 | None:intergenic |
| GTTCAGGCTACAAGATCCTA+TGG | 0.440809 | 3.3:-71163561 | MS.gene75621:CDS |
| GATCTGAACGCATGAAGTAT+AGG | 0.441700 | 3.3:+71163404 | None:intergenic |
| CGAGGCTTCTTAGCGGGTGG+AGG | 0.447598 | 3.3:+71164084 | None:intergenic |
| TTAGTTTGCTTTCTTGACAT+TGG | 0.450160 | 3.3:-71163703 | MS.gene75621:CDS |
| GTCTGGTCTCGCGTTGCTGT+AGG | 0.453658 | 3.3:-71163610 | MS.gene75621:CDS |
| CCTGGGTGTGTGGTGCCGAA+TGG | 0.455358 | 3.3:-71163028 | MS.gene75621:CDS |
| TGGCAAAACAATCAACTTTG+TGG | 0.460094 | 3.3:+71163744 | None:intergenic |
| CTGGGTGTGTGGTGCCGAAT+GGG | 0.467430 | 3.3:-71163027 | MS.gene75621:CDS |
| GTTGCCAGCTCGGTCAGTTA+AGG | 0.467547 | 3.3:-71163632 | MS.gene75621:CDS |
| TCAGTCAAATGTAGTGGCGC+TGG | 0.471171 | 3.3:+71163325 | None:intergenic |
| TCTGGTCTCGCGTTGCTGTA+GGG | 0.474671 | 3.3:-71163609 | MS.gene75621:CDS |
| TCTCTTCGAGGCTTCTTAGC+GGG | 0.476627 | 3.3:+71164078 | None:intergenic |
| GGCAAAACAATCAACTTTGT+GGG | 0.476939 | 3.3:+71163745 | None:intergenic |
| TCCGCTCTGTATGCCGTCAA+TGG | 0.480162 | 3.3:-71163924 | MS.gene75621:CDS |
| AACCCCTGTTGCCATATCAT+AGG | 0.480445 | 3.3:+71163366 | None:intergenic |
| TTGCCAGTGGCTTCTGCTGG+GGG | 0.482095 | 3.3:-71163727 | MS.gene75621:CDS |
| ACTTTCCAGCTGCCATTACA+AGG | 0.493916 | 3.3:+71164139 | None:intergenic |
| TGAGCAAGCCTTTGTTCCCT+AGG | 0.496907 | 3.3:+71163125 | None:intergenic |
| AGCCTCGAAGAGATCGAAGC+AGG | 0.497044 | 3.3:-71164068 | MS.gene75621:CDS |
| TGTGGCCTTGTAATGGCAGC+TGG | 0.498116 | 3.3:-71164144 | MS.gene75621:CDS |
| GATGGTCAGATCATGCTTGT+GGG | 0.503093 | 3.3:-71163283 | MS.gene75621:CDS |
| ACAACATAGCAAGGAAAGAA+TGG | 0.504668 | 3.3:-71163060 | MS.gene75621:CDS |
| ACAATCAACTTTGTGGGCAG+TGG | 0.506047 | 3.3:+71163751 | None:intergenic |
| TGAGATCGAAACAAATGAAT+CGG | 0.514096 | 3.3:-71163093 | MS.gene75621:CDS |
| GACGGCATACAGAGCGGAAG+CGG | 0.516102 | 3.3:+71163929 | None:intergenic |
| ATCTCTTCGAGGCTTCTTAG+CGG | 0.517356 | 3.3:+71164077 | None:intergenic |
| TGAAGTTAAGGGACAATGGC+AGG | 0.518383 | 3.3:+71163455 | None:intergenic |
| ATCCTATGGGTGGGTTGTGA+TGG | 0.519459 | 3.3:-71163547 | MS.gene75621:CDS |
| TTTCAAAGAAGGGTCATACA+TGG | 0.524726 | 3.3:+71163798 | None:intergenic |
| CACGTCAGAATGACTTGCCT+AGG | 0.525727 | 3.3:-71163142 | MS.gene75621:CDS |
| TTTGCCAGTGGCTTCTGCTG+GGG | 0.528979 | 3.3:-71163728 | MS.gene75621:CDS |
| CAGCTCGGTCAGTTAAGGTC+TGG | 0.530478 | 3.3:-71163627 | MS.gene75621:CDS |
| CCTTCAAGGAGTTGCCAGCT+CGG | 0.530770 | 3.3:-71163642 | MS.gene75621:CDS |
| AATGCCAAATATATGGTGCT+TGG | 0.531763 | 3.3:-71163179 | MS.gene75621:CDS |
| AGTTTGCGACCTGAAGTTAA+GGG | 0.541286 | 3.3:+71163444 | None:intergenic |
| CAAGCCACCGCGGTCAAGCA+TGG | 0.544564 | 3.3:+71162965 | None:intergenic |
| GCCTCGAAGAGATCGAAGCA+GGG | 0.544882 | 3.3:-71164067 | MS.gene75621:CDS |
| ATTCATTTGTTTCGATCTCA+GGG | 0.545165 | 3.3:+71163096 | None:intergenic |
| AGTCGTCCAGGAAACATGCC+TGG | 0.549750 | 3.3:-71163487 | MS.gene75621:CDS |
| CAGTCAAATGTAGTGGCGCT+GGG | 0.551715 | 3.3:+71163326 | None:intergenic |
| AGAGCTATCTTCGAGGTCAA+AGG | 0.555308 | 3.3:+71164179 | None:intergenic |
| CCAGCAAGGAATTCCATTGA+CGG | 0.555971 | 3.3:+71163911 | None:intergenic |
| CATCTACAGAGCTATCTTCG+AGG | 0.556179 | 3.3:+71164172 | None:intergenic |
| TTAGCGGGTGGAGGCTTCAG+AGG | 0.556937 | 3.3:+71164093 | None:intergenic |
| AAGAATGGCTGAAGGTTCCT+GGG | 0.558151 | 3.3:-71163045 | MS.gene75621:CDS |
| CTTCGAGGCTTCTTAGCGGG+TGG | 0.561487 | 3.3:+71164081 | None:intergenic |
| ATCGAAACAAATGAATCGGT+TGG | 0.564495 | 3.3:-71163089 | MS.gene75621:CDS |
| CCTTGAAGGATTGAGTTAGT+GGG | 0.570394 | 3.3:+71163656 | None:intergenic |
| CACACTCTAGCTGAGTATGA+TGG | 0.574409 | 3.3:-71163301 | MS.gene75621:CDS |
| TGACTGTTGTGGCCTTGTAA+TGG | 0.580674 | 3.3:-71164151 | MS.gene75621:CDS |
| TGGGTTATGGTGTAAAACCA+TGG | 0.586647 | 3.3:+71163841 | None:intergenic |
| GCTGTAGGGATGACAGTGAA+TGG | 0.589049 | 3.3:-71163595 | MS.gene75621:CDS |
| TGGTGCCGAATGGGAAGAAA+CGG | 0.591767 | 3.3:-71163018 | MS.gene75621:CDS |
| TGCAGAGAATGACACTCTTG+TGG | 0.591979 | 3.3:-71163216 | MS.gene75621:CDS |
| AAGTCCCGCAAGAAAATCCA+TGG | 0.594704 | 3.3:-71163858 | MS.gene75621:CDS |
| TCATCCATGCTTGACCGCGG+TGG | 0.597901 | 3.3:-71162969 | MS.gene75621:CDS |
| GCTGAAGGTTCCTGGGTGTG+TGG | 0.600893 | 3.3:-71163038 | MS.gene75621:CDS |
| TCATGCGTTCAGATCCAGAA+GGG | 0.601959 | 3.3:-71163396 | MS.gene75621:CDS |
| TGGTTACTTACAACATAGCA+AGG | 0.602101 | 3.3:-71163069 | MS.gene75621:CDS |
| GAGAATGACACTCTTGTGGA+AGG | 0.607066 | 3.3:-71163212 | MS.gene75621:CDS |
| ATGACTTGCCTAGGGAACAA+AGG | 0.609468 | 3.3:-71163133 | MS.gene75621:CDS |
| GGCTACAAGATCCTATGGGT+GGG | 0.610734 | 3.3:-71163556 | MS.gene75621:CDS |
| AGGCTACAAGATCCTATGGG+TGG | 0.612217 | 3.3:-71163557 | MS.gene75621:CDS |
| AAAGCTTTGAGAAGCCAGCA+AGG | 0.612786 | 3.3:+71163897 | None:intergenic |
| TGATGGTCAGATCATGCTTG+TGG | 0.614839 | 3.3:-71163284 | MS.gene75621:CDS |
| TTCCTATGATATGGCAACAG+GGG | 0.616895 | 3.3:-71163368 | MS.gene75621:CDS |
| GTGTGGTCAGTCAAATGTAG+TGG | 0.622699 | 3.3:+71163319 | None:intergenic |
| CTCCATCACAACCCACCCAT+AGG | 0.626354 | 3.3:+71163545 | None:intergenic |
| CAGACCTTAACTGACCGAGC+TGG | 0.631524 | 3.3:+71163628 | None:intergenic |
| AGAATATGAAGTTTATGACT+CGG | 0.638363 | 3.3:-71163524 | MS.gene75621:CDS |
| TTTCCTATGATATGGCAACA+GGG | 0.643758 | 3.3:-71163369 | MS.gene75621:CDS |
| ACGTCAGAATGACTTGCCTA+GGG | 0.644663 | 3.3:-71163141 | MS.gene75621:CDS |
| AATGACACTCTTGTGGAAGG+AGG | 0.644741 | 3.3:-71163209 | MS.gene75621:CDS |
| TTCAGGCTACAAGATCCTAT+GGG | 0.648233 | 3.3:-71163560 | MS.gene75621:CDS |
| AGCAAGGAAAGAATGGCTGA+AGG | 0.648548 | 3.3:-71163053 | MS.gene75621:CDS |
| CCATTCGGCACCACACACCC+AGG | 0.649604 | 3.3:+71163028 | None:intergenic |
| ATTTCATCCATGCTTGACCG+CGG | 0.649663 | 3.3:-71162972 | MS.gene75621:CDS |
| CGAATGGGAAGAAACGGCAG+TGG | 0.651054 | 3.3:-71163012 | MS.gene75621:CDS |
| GTGGGCAGTGGTGATATAGA+CGG | 0.653208 | 3.3:+71163763 | None:intergenic |
| TTCATGCGTTCAGATCCAGA+AGG | 0.658440 | 3.3:-71163397 | MS.gene75621:CDS |
| AGCCACGTGTGTATGCATAT+GGG | 0.660040 | 3.3:-71163242 | MS.gene75621:CDS |
| GCTATCTTCGAGGTCAAAGG+AGG | 0.661871 | 3.3:+71164182 | None:intergenic |
| CCTCGAAGAGATCGAAGCAG+GGG | 0.663964 | 3.3:-71164066 | MS.gene75621:CDS |
| CTCCCATATGCATACACACG+TGG | 0.665284 | 3.3:+71163240 | None:intergenic |
| TACATACATAGAAGTTACGA+TGG | 0.667085 | 3.3:+71163680 | None:intergenic |
| AGCTCTGTAGATGACTGTTG+TGG | 0.670937 | 3.3:-71164162 | MS.gene75621:CDS |
| CATGCGTTCAGATCCAGAAG+GGG | 0.671936 | 3.3:-71163395 | MS.gene75621:CDS |
| GACCTGAAGTTAAGGGACAA+TGG | 0.674409 | 3.3:+71163451 | None:intergenic |
| TCCATTGACGGCATACAGAG+CGG | 0.675929 | 3.3:+71163923 | None:intergenic |
| CATCATACTCAGCTAGAGTG+TGG | 0.675947 | 3.3:+71163302 | None:intergenic |
| AAACGGCAGTGGATAGCATG+TGG | 0.678505 | 3.3:-71163001 | MS.gene75621:CDS |
| CAAGGAAAATCAAGCCACCG+CGG | 0.733913 | 3.3:+71162955 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGGATAAACAAAAATATT+TGG | + | chr3.3:71163013-71163032 | None:intergenic | 15.0% |
| ! | AAGTTATGGAACAAGAAATA+TGG | - | chr3.3:71163212-71163231 | MS.gene75621:CDS | 25.0% |
| ! | AGAATATGAAGTTTATGACT+CGG | - | chr3.3:71163702-71163721 | MS.gene75621:CDS | 25.0% |
| ! | TAGAAAAACCAACATTTACA+GGG | + | chr3.3:71162971-71162990 | None:intergenic | 25.0% |
| !! | TTTTCCTGAAGATTTATTTG+AGG | - | chr3.3:71163240-71163259 | MS.gene75621:CDS | 25.0% |
| AATGGAAATTCAATCAGTTC+AGG | - | chr3.3:71163649-71163668 | MS.gene75621:CDS | 30.0% | |
| ATTCATTTGTTTCGATCTCA+GGG | + | chr3.3:71164133-71164152 | None:intergenic | 30.0% | |
| GTAGAAAAACCAACATTTAC+AGG | + | chr3.3:71162972-71162991 | None:intergenic | 30.0% | |
| TACATACATAGAAGTTACGA+TGG | + | chr3.3:71163549-71163568 | None:intergenic | 30.0% | |
| TAGACAGAATGCCAAATATA+TGG | - | chr3.3:71164040-71164059 | MS.gene75621:CDS | 30.0% | |
| TGAGATCGAAACAAATGAAT+CGG | - | chr3.3:71164133-71164152 | MS.gene75621:CDS | 30.0% | |
| ! | TAAAACCATGGATTTTCTTG+CGG | + | chr3.3:71163376-71163395 | None:intergenic | 30.0% |
| ! | TCCTGAATTCACATTTTCAT+GGG | + | chr3.3:71163407-71163426 | None:intergenic | 30.0% |
| ! | TTGTTTATCCATTTTGCAGA+TGG | - | chr3.3:71163020-71163039 | MS.gene75621:CDS | 30.0% |
| !! | ATTCACATTTTCATGGGTTA+TGG | + | chr3.3:71163401-71163420 | None:intergenic | 30.0% |
| !! | TTAGTTTGCTTTCTTGACAT+TGG | - | chr3.3:71163523-71163542 | MS.gene75621:CDS | 30.0% |
| !! | TTGGTTTTTCTACTTGACAT+GGG | - | chr3.3:71162979-71162998 | MS.gene75621:CDS | 30.0% |
| AAGTCCAAGCACCATATATT+TGG | + | chr3.3:71164054-71164073 | None:intergenic | 35.0% | |
| AATGCCAAATATATGGTGCT+TGG | - | chr3.3:71164047-71164066 | MS.gene75621:CDS | 35.0% | |
| ACAACATAGCAAGGAAAGAA+TGG | - | chr3.3:71164166-71164185 | MS.gene75621:CDS | 35.0% | |
| ACAGCCTCAAATAAATCTTC+AGG | + | chr3.3:71163247-71163266 | None:intergenic | 35.0% | |
| ACCCATGAAAATGTGAATTC+AGG | - | chr3.3:71163403-71163422 | MS.gene75621:CDS | 35.0% | |
| ATCCAGTACAAGTGAAGTTA+TGG | - | chr3.3:71163198-71163217 | MS.gene75621:CDS | 35.0% | |
| ATCGAAACAAATGAATCGGT+TGG | - | chr3.3:71164137-71164156 | MS.gene75621:CDS | 35.0% | |
| ATCTGAACGCATGAAGTATA+GGG | + | chr3.3:71163824-71163843 | None:intergenic | 35.0% | |
| GATTCATTTGTTTCGATCTC+AGG | + | chr3.3:71164134-71164153 | None:intergenic | 35.0% | |
| GGCAAAACAATCAACTTTGT+GGG | + | chr3.3:71163484-71163503 | None:intergenic | 35.0% | |
| TGGCAAAACAATCAACTTTG+TGG | + | chr3.3:71163485-71163504 | None:intergenic | 35.0% | |
| TGGTTACTTACAACATAGCA+AGG | - | chr3.3:71164157-71164176 | MS.gene75621:CDS | 35.0% | |
| TTCCATAACTTCACTTGTAC+TGG | + | chr3.3:71163203-71163222 | None:intergenic | 35.0% | |
| TTTCAAAGAAGGGTCATACA+TGG | + | chr3.3:71163431-71163450 | None:intergenic | 35.0% | |
| TTTCCTATGATATGGCAACA+GGG | - | chr3.3:71163857-71163876 | MS.gene75621:CDS | 35.0% | |
| ! | AAAACCATGGATTTTCTTGC+GGG | + | chr3.3:71163375-71163394 | None:intergenic | 35.0% |
| ! | AAGCTTTTCTAAGCAATGTG+CGG | - | chr3.3:71163345-71163364 | MS.gene75621:CDS | 35.0% |
| ! | ATATGGTGCTTGGACTTTTA+TGG | - | chr3.3:71164057-71164076 | MS.gene75621:CDS | 35.0% |
| ! | ATGACCCTTCTTTGAAAAAG+TGG | - | chr3.3:71163434-71163453 | MS.gene75621:CDS | 35.0% |
| ! | CTCCTGAATTCACATTTTCA+TGG | + | chr3.3:71163408-71163427 | None:intergenic | 35.0% |
| ! | CTGGAAAGTCTCATAGTTTT+AGG | - | chr3.3:71163101-71163120 | MS.gene75621:CDS | 35.0% |
| ! | TGGTTTTTCTACTTGACATG+GGG | - | chr3.3:71162980-71162999 | MS.gene75621:CDS | 35.0% |
| !! | AAAGTTGATTGTTTTGCCAG+TGG | - | chr3.3:71163486-71163505 | MS.gene75621:CDS | 35.0% |
| !!! | GATGCCACTTTTTCAAAGAA+GGG | + | chr3.3:71163441-71163460 | None:intergenic | 35.0% |
| !!! | GTTGGTTTTTCTACTTGACA+TGG | - | chr3.3:71162978-71162997 | MS.gene75621:CDS | 35.0% |
| !!! | TGATGCCACTTTTTCAAAGA+AGG | + | chr3.3:71163442-71163461 | None:intergenic | 35.0% |
| AGTTTGCGACCTGAAGTTAA+GGG | + | chr3.3:71163785-71163804 | None:intergenic | 40.0% | |
| CCTTGAAGGATTGAGTTAGT+GGG | + | chr3.3:71163573-71163592 | None:intergenic | 40.0% | |
| GATCTGAACGCATGAAGTAT+AGG | + | chr3.3:71163825-71163844 | None:intergenic | 40.0% | |
| GTTTCCTATGATATGGCAAC+AGG | - | chr3.3:71163856-71163875 | MS.gene75621:CDS | 40.0% | |
| TCCTTGAAGGATTGAGTTAG+TGG | + | chr3.3:71163574-71163593 | None:intergenic | 40.0% | |
| TGGGTTATGGTGTAAAACCA+TGG | + | chr3.3:71163388-71163407 | None:intergenic | 40.0% | |
| TTCAGGCTACAAGATCCTAT+GGG | - | chr3.3:71163666-71163685 | MS.gene75621:CDS | 40.0% | |
| TTCCTATGATATGGCAACAG+GGG | - | chr3.3:71163858-71163877 | MS.gene75621:CDS | 40.0% | |
| ! | AGGGGTTGTTTCCTATGATA+TGG | - | chr3.3:71163849-71163868 | MS.gene75621:CDS | 40.0% |
| !! | ATGACTCGGTGAAAAACTCT+TGG | - | chr3.3:71163716-71163735 | MS.gene75621:CDS | 40.0% |
| AAAAACTCTTGGAGTCGTCC+AGG | - | chr3.3:71163727-71163746 | MS.gene75621:CDS | 45.0% | |
| AAAGAATGGCTGAAGGTTCC+TGG | - | chr3.3:71164180-71164199 | MS.gene75621:CDS | 45.0% | |
| AACCCCTGTTGCCATATCAT+AGG | + | chr3.3:71163863-71163882 | None:intergenic | 45.0% | |
| AAGAATGGCTGAAGGTTCCT+GGG | - | chr3.3:71164181-71164200 | MS.gene75621:CDS | 45.0% | |
| AAGGAGGTCCATCTGCAAAA+TGG | + | chr3.3:71163031-71163050 | None:intergenic | 45.0% | |
| AAGTCCCGCAAGAAAATCCA+TGG | - | chr3.3:71163368-71163387 | MS.gene75621:CDS | 45.0% | |
| AATGACACTCTTGTGGAAGG+AGG | - | chr3.3:71164017-71164036 | MS.gene75621:CDS | 45.0% | |
| AATGGCAGGTTCATACTTCC+AGG | + | chr3.3:71163760-71163779 | None:intergenic | 45.0% | |
| ACAATCAACTTTGTGGGCAG+TGG | + | chr3.3:71163478-71163497 | None:intergenic | 45.0% | |
| ACTTTCCAGCTGCCATTACA+AGG | + | chr3.3:71163090-71163109 | None:intergenic | 45.0% | |
| AGAGCTATCTTCGAGGTCAA+AGG | + | chr3.3:71163050-71163069 | None:intergenic | 45.0% | |
| AGCAAGGAAAGAATGGCTGA+AGG | - | chr3.3:71164173-71164192 | MS.gene75621:CDS | 45.0% | |
| AGCCACGTGTGTATGCATAT+GGG | - | chr3.3:71163984-71164003 | MS.gene75621:CDS | 45.0% | |
| AGCTCTGTAGATGACTGTTG+TGG | - | chr3.3:71163064-71163083 | MS.gene75621:CDS | 45.0% | |
| ATACTTCCAGGCATGTTTCC+TGG | + | chr3.3:71163748-71163767 | None:intergenic | 45.0% | |
| ATCTCTTCGAGGCTTCTTAG+CGG | + | chr3.3:71163152-71163171 | None:intergenic | 45.0% | |
| ATGATATGGCAACAGGGGTT+TGG | - | chr3.3:71163863-71163882 | MS.gene75621:CDS | 45.0% | |
| ATTTCATCCATGCTTGACCG+CGG | - | chr3.3:71164254-71164273 | MS.gene75621:intron | 45.0% | |
| CACACTCTAGCTGAGTATGA+TGG | - | chr3.3:71163925-71163944 | MS.gene75621:CDS | 45.0% | |
| CAGTTTGCGACCTGAAGTTA+AGG | + | chr3.3:71163786-71163805 | None:intergenic | 45.0% | |
| CATCATACTCAGCTAGAGTG+TGG | + | chr3.3:71163927-71163946 | None:intergenic | 45.0% | |
| CATCTACAGAGCTATCTTCG+AGG | + | chr3.3:71163057-71163076 | None:intergenic | 45.0% | |
| CCAGCAAGGAATTCCATTGA+CGG | + | chr3.3:71163318-71163337 | None:intergenic | 45.0% | |
| CCCACTAACTCAATCCTTCA+AGG | - | chr3.3:71163570-71163589 | MS.gene75621:CDS | 45.0% | |
| GACCTGAAGTTAAGGGACAA+TGG | + | chr3.3:71163778-71163797 | None:intergenic | 45.0% | |
| GAGAATGACACTCTTGTGGA+AGG | - | chr3.3:71164014-71164033 | MS.gene75621:CDS | 45.0% | |
| GATGGTCAGATCATGCTTGT+GGG | - | chr3.3:71163943-71163962 | MS.gene75621:CDS | 45.0% | |
| GTGTGGTCAGTCAAATGTAG+TGG | + | chr3.3:71163910-71163929 | None:intergenic | 45.0% | |
| GTTCAGGCTACAAGATCCTA+TGG | - | chr3.3:71163665-71163684 | MS.gene75621:CDS | 45.0% | |
| TCATAGGAAACAACCCCTTC+TGG | + | chr3.3:71163847-71163866 | None:intergenic | 45.0% | |
| TCATGCGTTCAGATCCAGAA+GGG | - | chr3.3:71163830-71163849 | MS.gene75621:CDS | 45.0% | |
| TGAAGTTAAGGGACAATGGC+AGG | + | chr3.3:71163774-71163793 | None:intergenic | 45.0% | |
| TGATGGTCAGATCATGCTTG+TGG | - | chr3.3:71163942-71163961 | MS.gene75621:CDS | 45.0% | |
| TGCAGAGAATGACACTCTTG+TGG | - | chr3.3:71164010-71164029 | MS.gene75621:CDS | 45.0% | |
| TGCCATTGTCCCTTAACTTC+AGG | - | chr3.3:71163773-71163792 | MS.gene75621:CDS | 45.0% | |
| TTCATGCGTTCAGATCCAGA+AGG | - | chr3.3:71163829-71163848 | MS.gene75621:CDS | 45.0% | |
| ! | ACGTCAGAATGACTTGCCTA+GGG | - | chr3.3:71164085-71164104 | MS.gene75621:CDS | 45.0% |
| ! | ATGACTTGCCTAGGGAACAA+AGG | - | chr3.3:71164093-71164112 | MS.gene75621:CDS | 45.0% |
| !! | AAAGCTTTGAGAAGCCAGCA+AGG | + | chr3.3:71163332-71163351 | None:intergenic | 45.0% |
| !! | TGACTGTTGTGGCCTTGTAA+TGG | - | chr3.3:71163075-71163094 | MS.gene75621:CDS | 45.0% |
| AAACGGCAGTGGATAGCATG+TGG | - | chr3.3:71164225-71164244 | MS.gene75621:intron | 50.0% | |
| AAGCGGAAAAACGCGGCAAT+GGG | + | chr3.3:71163283-71163302 | None:intergenic | 50.0% | |
| AGGCTACAAGATCCTATGGG+TGG | - | chr3.3:71163669-71163688 | MS.gene75621:CDS | 50.0% | |
| CAGCCACGTGTGTATGCATA+TGG | - | chr3.3:71163983-71164002 | MS.gene75621:CDS | 50.0% | |
| CAGTCAAATGTAGTGGCGCT+GGG | + | chr3.3:71163903-71163922 | None:intergenic | 50.0% | |
| CATGCGTTCAGATCCAGAAG+GGG | - | chr3.3:71163831-71163850 | MS.gene75621:CDS | 50.0% | |
| CCGTCAATGGAATTCCTTGC+TGG | - | chr3.3:71163315-71163334 | MS.gene75621:CDS | 50.0% | |
| CTCCCATATGCATACACACG+TGG | + | chr3.3:71163989-71164008 | None:intergenic | 50.0% | |
| GCTATCTTCGAGGTCAAAGG+AGG | + | chr3.3:71163047-71163066 | None:intergenic | 50.0% | |
| GCTGTAGGGATGACAGTGAA+TGG | - | chr3.3:71163631-71163650 | MS.gene75621:CDS | 50.0% | |
| GGCTACAAGATCCTATGGGT+GGG | - | chr3.3:71163670-71163689 | MS.gene75621:CDS | 50.0% | |
| GTGGGCAGTGGTGATATAGA+CGG | + | chr3.3:71163466-71163485 | None:intergenic | 50.0% | |
| TCAGTCAAATGTAGTGGCGC+TGG | + | chr3.3:71163904-71163923 | None:intergenic | 50.0% | |
| TCCATTGACGGCATACAGAG+CGG | + | chr3.3:71163306-71163325 | None:intergenic | 50.0% | |
| TCTCTTCGAGGCTTCTTAGC+GGG | + | chr3.3:71163151-71163170 | None:intergenic | 50.0% | |
| TGAGCAAGCCTTTGTTCCCT+AGG | + | chr3.3:71164104-71164123 | None:intergenic | 50.0% | |
| ! | CACGTCAGAATGACTTGCCT+AGG | - | chr3.3:71164084-71164103 | MS.gene75621:CDS | 50.0% |
| ! | CACTGCCGTTTCTTCCCATT+CGG | + | chr3.3:71164216-71164235 | None:intergenic | 50.0% |
| ! | TGGTGCCGAATGGGAAGAAA+CGG | - | chr3.3:71164208-71164227 | MS.gene75621:intron | 50.0% |
| ! | TTTTGCCAGTGGCTTCTGCT+GGG | - | chr3.3:71163497-71163516 | MS.gene75621:CDS | 50.0% |
| !! | ATCCTATGGGTGGGTTGTGA+TGG | - | chr3.3:71163679-71163698 | MS.gene75621:CDS | 50.0% |
| AGAGCGGAAGCGGAAAAACG+CGG | + | chr3.3:71163290-71163309 | None:intergenic | 55.0% | |
| AGCCTCGAAGAGATCGAAGC+AGG | - | chr3.3:71163158-71163177 | MS.gene75621:CDS | 55.0% | |
| AGTCGTCCAGGAAACATGCC+TGG | - | chr3.3:71163739-71163758 | MS.gene75621:CDS | 55.0% | |
| CAGACCTTAACTGACCGAGC+TGG | + | chr3.3:71163601-71163620 | None:intergenic | 55.0% | |
| CAGCTCGGTCAGTTAAGGTC+TGG | - | chr3.3:71163599-71163618 | MS.gene75621:CDS | 55.0% | |
| CCTCGAAGAGATCGAAGCAG+GGG | - | chr3.3:71163160-71163179 | MS.gene75621:CDS | 55.0% | |
| CCTTCAAGGAGTTGCCAGCT+CGG | - | chr3.3:71163584-71163603 | MS.gene75621:CDS | 55.0% | |
| CGAAGCAGGGGCAAATCATC+AGG | - | chr3.3:71163172-71163191 | MS.gene75621:CDS | 55.0% | |
| CGAATGGGAAGAAACGGCAG+TGG | - | chr3.3:71164214-71164233 | MS.gene75621:intron | 55.0% | |
| CTCCATCACAACCCACCCAT+AGG | + | chr3.3:71163684-71163703 | None:intergenic | 55.0% | |
| GAAGCGGAAAAACGCGGCAA+TGG | + | chr3.3:71163284-71163303 | None:intergenic | 55.0% | |
| GCCTCGAAGAGATCGAAGCA+GGG | - | chr3.3:71163159-71163178 | MS.gene75621:CDS | 55.0% | |
| GTTGCCAGCTCGGTCAGTTA+AGG | - | chr3.3:71163594-71163613 | MS.gene75621:CDS | 55.0% | |
| TCCGCTCTGTATGCCGTCAA+TGG | - | chr3.3:71163302-71163321 | MS.gene75621:CDS | 55.0% | |
| TCTGGTCTCGCGTTGCTGTA+GGG | - | chr3.3:71163617-71163636 | MS.gene75621:CDS | 55.0% | |
| TTTGCCAGTGGCTTCTGCTG+GGG | - | chr3.3:71163498-71163517 | MS.gene75621:CDS | 55.0% | |
| ! | GTTTTGCCAGTGGCTTCTGC+TGG | - | chr3.3:71163496-71163515 | MS.gene75621:CDS | 55.0% |
| !! | TGTGGCCTTGTAATGGCAGC+TGG | - | chr3.3:71163082-71163101 | MS.gene75621:CDS | 55.0% |
| AAACCCCCAGCAGAAGCCAC+TGG | + | chr3.3:71163505-71163524 | None:intergenic | 60.0% | |
| CCCCTGCTTCGATCTCTTCG+AGG | + | chr3.3:71163163-71163182 | None:intergenic | 60.0% | |
| CCGAGCTGGCAACTCCTTGA+AGG | + | chr3.3:71163587-71163606 | None:intergenic | 60.0% | |
| CTTCGAGGCTTCTTAGCGGG+TGG | + | chr3.3:71163148-71163167 | None:intergenic | 60.0% | |
| GACGGCATACAGAGCGGAAG+CGG | + | chr3.3:71163300-71163319 | None:intergenic | 60.0% | |
| GCTGAAGGTTCCTGGGTGTG+TGG | - | chr3.3:71164188-71164207 | MS.gene75621:CDS | 60.0% | |
| GTCTGGTCTCGCGTTGCTGT+AGG | - | chr3.3:71163616-71163635 | MS.gene75621:CDS | 60.0% | |
| TCATCCATGCTTGACCGCGG+TGG | - | chr3.3:71164257-71164276 | MS.gene75621:intron | 60.0% | |
| TTGCCAGTGGCTTCTGCTGG+GGG | - | chr3.3:71163499-71163518 | MS.gene75621:CDS | 60.0% | |
| ! | CTGGGTGTGTGGTGCCGAAT+GGG | - | chr3.3:71164199-71164218 | MS.gene75621:intron | 60.0% |
| ! | TTAGCGGGTGGAGGCTTCAG+AGG | + | chr3.3:71163136-71163155 | None:intergenic | 60.0% |
| CAAGCCACCGCGGTCAAGCA+TGG | + | chr3.3:71164264-71164283 | None:intergenic | 65.0% | |
| CCATTCGGCACCACACACCC+AGG | + | chr3.3:71164201-71164220 | None:intergenic | 65.0% | |
| ! | CCTGGGTGTGTGGTGCCGAA+TGG | - | chr3.3:71164198-71164217 | MS.gene75621:intron | 65.0% |
| ! | CGAGGCTTCTTAGCGGGTGG+AGG | + | chr3.3:71163145-71163164 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 71162964 | 71164284 | 71162964 | ID=MS.gene75621 |
| chr3.3 | mRNA | 71162964 | 71164284 | 71162964 | ID=MS.gene75621.t1;Parent=MS.gene75621 |
| chr3.3 | exon | 71164277 | 71164284 | 71164277 | ID=MS.gene75621.t1.exon1;Parent=MS.gene75621.t1 |
| chr3.3 | CDS | 71164277 | 71164284 | 71164277 | ID=cds.MS.gene75621.t1;Parent=MS.gene75621.t1 |
| chr3.3 | exon | 71162964 | 71164209 | 71162964 | ID=MS.gene75621.t1.exon2;Parent=MS.gene75621.t1 |
| chr3.3 | CDS | 71162964 | 71164209 | 71162964 | ID=cds.MS.gene75621.t1;Parent=MS.gene75621.t1 |
| Gene Sequence |
| Protein sequence |
