AlfalfaGEDB | MS.gene76160

M. sativa cultivar XinJiangDaYe / MS.gene76160

KEGG: -
GO: -
PFAM: PF08246.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76160.t1	PNX71632.1	58.3	72	30	0	22	93	25	96	2.50E-15	91.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76160.t1	Q9LXW3	41.8	67	39	0	27	93	36	102	1.5e-08	60.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76160.t1	A0A2K3KZC1	58.3	72	30	0	22	93	25	96	1.8e-15	91.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049217	MS.gene76160	-0.815588	8.56E-52	-1.69E-46
MS.gene049222	MS.gene76160	-0.823847	1.11E-53	-1.69E-46
MS.gene050165	MS.gene76160	-0.802348	5.85E-49	-1.69E-46
MS.gene050444	MS.gene76160	-0.848094	7.63E-60	-1.69E-46
MS.gene05079	MS.gene76160	0.801164	1.02E-48	-1.69E-46
MS.gene050809	MS.gene76160	0.805316	1.42E-49	-1.69E-46
MS.gene05080	MS.gene76160	-0.818099	2.34E-52	-1.69E-46
MS.gene050868	MS.gene76160	-0.815058	1.12E-51	-1.69E-46
MS.gene050871	MS.gene76160	-0.81461	1.41E-51	-1.69E-46
MS.gene050904	MS.gene76160	-0.812332	4.47E-51	-1.69E-46
MS.gene051838	MS.gene76160	-0.831511	1.60E-55	-1.69E-46
MS.gene052050	MS.gene76160	0.813431	2.57E-51	-1.69E-46
MS.gene052164	MS.gene76160	-0.805084	1.58E-49	-1.69E-46
MS.gene052870	MS.gene76160	-0.82929	5.59E-55	-1.69E-46
MS.gene052872	MS.gene76160	-0.832888	7.31E-56	-1.69E-46
MS.gene053632	MS.gene76160	-0.826563	2.53E-54	-1.69E-46
MS.gene053776	MS.gene76160	0.83262	8.52E-56	-1.69E-46
MS.gene053824	MS.gene76160	-0.813035	3.14E-51	-1.69E-46
MS.gene054706	MS.gene76160	0.815117	1.09E-51	-1.69E-46
MS.gene05494	MS.gene76160	-0.813402	2.61E-51	-1.69E-46
MS.gene055577	MS.gene76160	-0.836961	6.88E-57	-1.69E-46
MS.gene055748	MS.gene76160	-0.809497	1.84E-50	-1.69E-46
MS.gene055751	MS.gene76160	-0.804437	2.16E-49	-1.69E-46
MS.gene055941	MS.gene76160	-0.811617	6.40E-51	-1.69E-46
MS.gene056387	MS.gene76160	-0.814255	1.69E-51	-1.69E-46
MS.gene056449	MS.gene76160	-0.811727	6.06E-51	-1.69E-46
MS.gene056903	MS.gene76160	-0.802628	5.13E-49	-1.69E-46
MS.gene058158	MS.gene76160	0.804611	1.99E-49	-1.69E-46
MS.gene058773	MS.gene76160	-0.828959	6.73E-55	-1.69E-46
MS.gene059023	MS.gene76160	-0.819906	9.08E-53	-1.69E-46
MS.gene059102	MS.gene76160	0.802983	4.33E-49	-1.69E-46
MS.gene059677	MS.gene76160	-0.800448	1.43E-48	-1.69E-46
MS.gene059718	MS.gene76160	-0.814416	1.56E-51	-1.69E-46
MS.gene060762	MS.gene76160	-0.829259	5.69E-55	-1.69E-46
MS.gene06111	MS.gene76160	-0.810915	9.09E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76160.t1	MTR_7g086240	52.500	80	34	1	11	90	17	92	1.29e-23	87.8
MS.gene76160.t1	MTR_3g112430	55.556	63	28	0	27	89	31	93	2.81e-18	79.3
MS.gene76160.t1	MTR_2g064475	54.688	64	29	0	33	96	17	80	8.70e-17	74.7
MS.gene76160.t1	MTR_2g067560	48.438	64	33	0	32	95	16	79	6.75e-15	69.7
MS.gene76160.t1	MTR_2g067900	49.206	63	32	0	33	95	17	79	6.16e-14	66.6
MS.gene76160.t1	MTR_2g066980	48.438	64	33	0	33	96	35	98	3.65e-13	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76160.t1	AT1G09850	45.833	72	38	1	27	97	27	98	8.47e-16	72.4
MS.gene76160.t1	AT3G43960	41.791	67	39	0	27	93	36	102	6.83e-13	63.9
MS.gene76160.t1	AT4G35350	51.515	66	31	1	28	93	47	111	9.06e-13	63.5
MS.gene76160.t1	AT4G35350	51.515	66	31	1	28	93	47	111	9.83e-13	63.2

Find 27 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCAATGGAACAACCATAAT+TGG	0.195557	2.3:+28431632	None:intergenic
TTGGTTGTGTTGAGTGATTC+TGG	0.214560	2.3:+28431722	None:intergenic
GAGGAATTTACGTCCAATTA+TGG	0.252320	2.3:-28431645	MS.gene76160:CDS
TTCTTGAAGCTTGGAGTTGG+AGG	0.392872	2.3:+28431980	None:intergenic
AGAATAACAAACCGATGGTT+GGG	0.404177	2.3:+28431947	None:intergenic
CTGTTCTTGAAGCTTGGAGT+TGG	0.411197	2.3:+28431977	None:intergenic
CCGGATGATCTTGAAAAGTT+AGG	0.423858	2.3:-28431594	MS.gene76160:CDS
AAAACTTCATTGATGTTTGA+CGG	0.433536	2.3:+28431563	None:intergenic
TCCATGAAATATTTGAAAAG+TGG	0.434083	2.3:-28431821	MS.gene76160:CDS
AAACTTCATTGATGTTTGAC+GGG	0.435142	2.3:+28431564	None:intergenic
AGGAATCTGTTCTTGAAGCT+TGG	0.443942	2.3:+28431971	None:intergenic
TGAATATAAGGCTCCTGGTT+CGG	0.465353	2.3:-28431847	MS.gene76160:intron
AATTGTGAATATAAGGCTCC+TGG	0.479700	2.3:-28431852	MS.gene76160:intron
CGAAGGTGAGATCAGAGAAC+TGG	0.488939	2.3:+28431667	None:intergenic
TGATTTCAACGATGTCCATC+CGG	0.494998	2.3:-28431613	MS.gene76160:CDS
TAGAATAACAAACCGATGGT+TGG	0.501861	2.3:+28431946	None:intergenic
GTTCTCTGATCTCACCTTCG+AGG	0.507841	2.3:-28431664	MS.gene76160:CDS
ATGGACATCGTTGAAATCAA+TGG	0.510440	2.3:+28431617	None:intergenic
CGACAAAACATATTCTTCAA+AGG	0.513620	2.3:-28431787	MS.gene76160:CDS
GAATAACAAACCGATGGTTG+GGG	0.564507	2.3:+28431948	None:intergenic
ACAGATTCCTCCCCAACCAT+CGG	0.584110	2.3:-28431958	MS.gene76160:intron
ATTTCATGGATGTCCGAACC+AGG	0.584248	2.3:+28431834	None:intergenic
TGAGATCAGAGAACTGGTTG+AGG	0.597092	2.3:+28431673	None:intergenic
AATATTTGAAAAGTGGTGCA+AGG	0.602264	2.3:-28431814	MS.gene76160:CDS
TAACAAACCGATGGTTGGGG+AGG	0.622332	2.3:+28431951	None:intergenic
ATTGGACGTAAATTCCTCGA+AGG	0.638330	2.3:+28431650	None:intergenic
AACTTCATTGATGTTTGACG+GGG	0.670693	2.3:+28431565	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TAATTTTAATTGTGAATATA+AGG	-	chr2.3:28431712-28431731	MS.gene76160:CDS	10.0%
!	AAAATCATGAGAGAAATTGA+AGG	+	chr2.3:28431653-28431672	None:intergenic	25.0%
!	AAATCATGAGAGAAATTGAA+GGG	+	chr2.3:28431652-28431671	None:intergenic	25.0%
!	TCCATGAAATATTTGAAAAG+TGG	-	chr2.3:28431750-28431769	MS.gene76160:CDS	25.0%
!!!	ACCACTTTTCAAATATTTCA+TGG	+	chr2.3:28431754-28431773	None:intergenic	25.0%
	AATATTTGAAAAGTGGTGCA+AGG	-	chr2.3:28431757-28431776	MS.gene76160:CDS	30.0%
	ATCAATGGAACAACCATAAT+TGG	+	chr2.3:28431942-28431961	None:intergenic	30.0%
	CGACAAAACATATTCTTCAA+AGG	-	chr2.3:28431784-28431803	MS.gene76160:CDS	30.0%
!	AAAAATTCTGGTTACACTCT+AGG	-	chr2.3:28431875-28431894	MS.gene76160:intron	30.0%
!!	TGTAACCAGAATTTTTCAGT+TGG	+	chr2.3:28431871-28431890	None:intergenic	30.0%
	AATTGTGAATATAAGGCTCC+TGG	-	chr2.3:28431719-28431738	MS.gene76160:CDS	35.0%
	AGAATAACAAACCGATGGTT+GGG	+	chr2.3:28431627-28431646	None:intergenic	35.0%
	ATGGACATCGTTGAAATCAA+TGG	+	chr2.3:28431957-28431976	None:intergenic	35.0%
	CAAAGGAAGAAAAACAGTAC+AGG	-	chr2.3:28431801-28431820	MS.gene76160:CDS	35.0%
	CACAACCAACTGAAAAATTC+TGG	-	chr2.3:28431863-28431882	MS.gene76160:intron	35.0%
	GAGGAATTTACGTCCAATTA+TGG	-	chr2.3:28431926-28431945	MS.gene76160:intron	35.0%
	TAGAATAACAAACCGATGGT+TGG	+	chr2.3:28431628-28431647	None:intergenic	35.0%
!!	CCTAACTTTTCAAGATCATC+CGG	+	chr2.3:28431980-28431999	None:intergenic	35.0%
	AGGAATCTGTTCTTGAAGCT+TGG	+	chr2.3:28431603-28431622	None:intergenic	40.0%
	ATTGGACGTAAATTCCTCGA+AGG	+	chr2.3:28431924-28431943	None:intergenic	40.0%
	CCGGATGATCTTGAAAAGTT+AGG	-	chr2.3:28431977-28431996	MS.gene76160:CDS	40.0%
	GAATAACAAACCGATGGTTG+GGG	+	chr2.3:28431626-28431645	None:intergenic	40.0%
	GGGATAGAATAACAAACCGA+TGG	+	chr2.3:28431632-28431651	None:intergenic	40.0%
	TGAATATAAGGCTCCTGGTT+CGG	-	chr2.3:28431724-28431743	MS.gene76160:CDS	40.0%
	TGATTTCAACGATGTCCATC+CGG	-	chr2.3:28431958-28431977	MS.gene76160:intron	40.0%
	TTGGTTGTGTTGAGTGATTC+TGG	+	chr2.3:28431852-28431871	None:intergenic	40.0%
!!	ACTTTTCAAGATCATCCGGA+TGG	+	chr2.3:28431976-28431995	None:intergenic	40.0%
	ATTTCATGGATGTCCGAACC+AGG	+	chr2.3:28431740-28431759	None:intergenic	45.0%
	CTGTTCTTGAAGCTTGGAGT+TGG	+	chr2.3:28431597-28431616	None:intergenic	45.0%
	TGAGATCAGAGAACTGGTTG+AGG	+	chr2.3:28431901-28431920	None:intergenic	45.0%
	TTCTTGAAGCTTGGAGTTGG+AGG	+	chr2.3:28431594-28431613	None:intergenic	45.0%
	ACAGATTCCTCCCCAACCAT+CGG	-	chr2.3:28431613-28431632	MS.gene76160:CDS	50.0%
	CGAAGGTGAGATCAGAGAAC+TGG	+	chr2.3:28431907-28431926	None:intergenic	50.0%
	GTTCTCTGATCTCACCTTCG+AGG	-	chr2.3:28431907-28431926	MS.gene76160:intron	50.0%
	TAACAAACCGATGGTTGGGG+AGG	+	chr2.3:28431623-28431642	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	28431569	28432024	28431569	ID=MS.gene76160
chr2.3	mRNA	28431569	28432024	28431569	ID=MS.gene76160.t1;Parent=MS.gene76160
chr2.3	exon	28431959	28432024	28431959	ID=MS.gene76160.t1.exon1;Parent=MS.gene76160.t1
chr2.3	CDS	28431959	28432024	28431959	ID=cds.MS.gene76160.t1;Parent=MS.gene76160.t1
chr2.3	exon	28431569	28431859	28431569	ID=MS.gene76160.t1.exon2;Parent=MS.gene76160.t1
chr2.3	CDS	28431569	28431859	28431569	ID=cds.MS.gene76160.t1;Parent=MS.gene76160.t1

Gene Sequence

>MS.gene76160

ATGGAAGTAAACAAAGAGCTAACCTCCAACTCCAAGCTTCAAGAACAGATTCCTCCCCAACCATCGGTTTGTTATTCTATCCCTTCAATTTCTCTCATGATTTTTGTTTAATTGATTGATTTTCTTTGTTAATTTATACTTTGTAATTTTAATTGTGAATATAAGGCTCCTGGTTCGGACATCCATGAAATATTTGAAAAGTGGTGCAAGGAATTCGACAAAACATATTCTTCAAAGGAAGAAAAACAGTACAGGTTCAAAGTGTTTGAAGAAAGATATGCCAGAATCACTCAACACAACCAACTGAAAAATTCTGGTTACACTCTAGGCCTCAACCAGTTCTCTGATCTCACCTTCGAGGAATTTACGTCCAATTATGGTTGTTCCATTGATTTCAACGATGTCCATCCGGATGATCTTGAAAAGTTAGGCATTAACCCCGTCAAACATCAATGA

Protein sequence

>MS.gene76160.t1

MEVNKELTSNSKLQEQIPPQPSAPGSDIHEIFEKWCKEFDKTYSSKEEKQYRFKVFEERYARITQHNQLKNSGYTLGLNQFSDLTFEEFTSNYGCSIDFNDVHPDDLEKLGINPVKHQ