Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059102.t1 | ACJ85371.1 | 97.9 | 234 | 3 | 1 | 1 | 234 | 1 | 232 | 3.20E-123 | 451.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059102.t1 | B7FKT7 | 97.9 | 234 | 3 | 1 | 1 | 234 | 1 | 232 | 2.3e-123 | 451.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049217 | MS.gene059102 | -0.811248 | 7.70E-51 | -1.69E-46 |
| MS.gene049222 | MS.gene059102 | -0.800674 | 1.29E-48 | -1.69E-46 |
| MS.gene049402 | MS.gene059102 | 0.831143 | 1.97E-55 | -1.69E-46 |
| MS.gene050163 | MS.gene059102 | -0.856296 | 3.54E-62 | -1.69E-46 |
| MS.gene050165 | MS.gene059102 | -0.858247 | 9.39E-63 | -1.69E-46 |
| MS.gene050333 | MS.gene059102 | -0.800859 | 1.18E-48 | -1.69E-46 |
| MS.gene050504 | MS.gene059102 | -0.818333 | 2.07E-52 | -1.69E-46 |
| MS.gene050612 | MS.gene059102 | -0.807686 | 4.48E-50 | -1.69E-46 |
| MS.gene05100 | MS.gene059102 | -0.808131 | 3.60E-50 | -1.69E-46 |
| MS.gene051810 | MS.gene059102 | -0.802168 | 6.38E-49 | -1.69E-46 |
| MS.gene052379 | MS.gene059102 | -0.801357 | 9.36E-49 | -1.69E-46 |
| MS.gene055748 | MS.gene059102 | -0.815501 | 8.95E-52 | -1.69E-46 |
| MS.gene055751 | MS.gene059102 | -0.806889 | 6.61E-50 | -1.69E-46 |
| MS.gene056856 | MS.gene059102 | 0.808572 | 2.90E-50 | -1.69E-46 |
| MS.gene056903 | MS.gene059102 | -0.817656 | 2.95E-52 | -1.69E-46 |
| MS.gene057246 | MS.gene059102 | -0.824214 | 9.11E-54 | -1.69E-46 |
| MS.gene059022 | MS.gene059102 | -0.800427 | 1.45E-48 | -1.69E-46 |
| MS.gene059079 | MS.gene059102 | -0.801882 | 7.30E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene059479 | 0.844241 | 8.54E-59 | -1.69E-46 |
| MS.gene059102 | MS.gene059677 | -0.824685 | 7.06E-54 | -1.69E-46 |
| MS.gene059102 | MS.gene060762 | -0.818283 | 2.12E-52 | -1.69E-46 |
| MS.gene059102 | MS.gene061150 | -0.808794 | 2.60E-50 | -1.69E-46 |
| MS.gene059102 | MS.gene06223 | -0.829488 | 5.00E-55 | -1.69E-46 |
| MS.gene059102 | MS.gene062903 | -0.802362 | 5.81E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene063080 | -0.8113 | 7.50E-51 | -1.69E-46 |
| MS.gene059102 | MS.gene063091 | -0.806438 | 8.23E-50 | -1.69E-46 |
| MS.gene059102 | MS.gene06332 | -0.803743 | 3.01E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene06336 | -0.801027 | 1.09E-48 | -1.69E-46 |
| MS.gene059102 | MS.gene064800 | -0.808187 | 3.50E-50 | -1.69E-46 |
| MS.gene059102 | MS.gene064862 | -0.810548 | 1.09E-50 | -1.69E-46 |
| MS.gene059102 | MS.gene064867 | -0.823463 | 1.37E-53 | -1.69E-46 |
| MS.gene059102 | MS.gene065658 | -0.806681 | 7.31E-50 | -1.69E-46 |
| MS.gene059102 | MS.gene066565 | -0.807269 | 5.49E-50 | -1.69E-46 |
| MS.gene059102 | MS.gene067047 | -0.818427 | 1.97E-52 | -1.69E-46 |
| MS.gene059102 | MS.gene069995 | -0.812975 | 3.23E-51 | -1.69E-46 |
| MS.gene059102 | MS.gene071308 | -0.80277 | 4.79E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene072086 | -0.814419 | 1.56E-51 | -1.69E-46 |
| MS.gene059102 | MS.gene072176 | -0.801957 | 7.05E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene072472 | -0.818138 | 2.29E-52 | -1.69E-46 |
| MS.gene059102 | MS.gene072966 | -0.832972 | 6.96E-56 | -1.69E-46 |
| MS.gene059102 | MS.gene08658 | 0.810878 | 9.26E-51 | -1.69E-46 |
| MS.gene059102 | MS.gene09072 | 0.814111 | 1.82E-51 | -1.69E-46 |
| MS.gene059102 | MS.gene09661 | -0.818925 | 1.52E-52 | -1.69E-46 |
| MS.gene059102 | MS.gene09720 | -0.835902 | 1.28E-56 | -1.69E-46 |
| MS.gene059102 | MS.gene09722 | -0.855291 | 6.96E-62 | -1.69E-46 |
| MS.gene059102 | MS.gene21474 | -0.814432 | 1.55E-51 | -1.69E-46 |
| MS.gene059102 | MS.gene22275 | -0.801048 | 1.08E-48 | -1.69E-46 |
| MS.gene059102 | MS.gene22454 | -0.801724 | 7.87E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene22826 | -0.801439 | 9.00E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene23018 | -0.824318 | 8.61E-54 | -1.69E-46 |
| MS.gene059102 | MS.gene23334 | -0.803141 | 4.02E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene23417 | -0.804851 | 1.77E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene23996 | -0.813062 | 3.10E-51 | -1.69E-46 |
| MS.gene059102 | MS.gene24025 | -0.801742 | 7.80E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene24329 | -0.8199 | 9.11E-53 | -1.69E-46 |
| MS.gene059102 | MS.gene24420 | 0.813719 | 2.22E-51 | -1.69E-46 |
| MS.gene059102 | MS.gene24674 | -0.805357 | 1.39E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene24767 | -0.808044 | 3.76E-50 | -1.69E-46 |
| MS.gene059102 | MS.gene25483 | -0.829513 | 4.93E-55 | -1.69E-46 |
| MS.gene059102 | MS.gene25666 | -0.817063 | 4.00E-52 | -1.69E-46 |
| MS.gene059102 | MS.gene25675 | -0.819023 | 1.44E-52 | -1.69E-46 |
| MS.gene059102 | MS.gene25677 | -0.820137 | 8.04E-53 | -1.69E-46 |
| MS.gene059102 | MS.gene26384 | -0.804243 | 2.37E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene26447 | -0.804386 | 2.21E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene26662 | -0.801775 | 7.68E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene26698 | -0.805604 | 1.23E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene26917 | -0.800253 | 1.57E-48 | -1.69E-46 |
| MS.gene059102 | MS.gene27061 | -0.813849 | 2.08E-51 | -1.69E-46 |
| MS.gene059102 | MS.gene27768 | 0.819608 | 1.06E-52 | -1.69E-46 |
| MS.gene059102 | MS.gene28267 | -0.826797 | 2.23E-54 | -1.69E-46 |
| MS.gene059102 | MS.gene29338 | -0.800941 | 1.14E-48 | -1.69E-46 |
| MS.gene059102 | MS.gene29927 | -0.810041 | 1.40E-50 | -1.69E-46 |
| MS.gene059102 | MS.gene31165 | 0.808642 | 2.80E-50 | -1.69E-46 |
| MS.gene059102 | MS.gene31988 | -0.809793 | 1.59E-50 | -1.69E-46 |
| MS.gene059102 | MS.gene32234 | -0.802697 | 4.96E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene32758 | -0.816216 | 6.20E-52 | -1.69E-46 |
| MS.gene059102 | MS.gene33825 | -0.809187 | 2.14E-50 | -1.69E-46 |
| MS.gene059102 | MS.gene34678 | -0.810662 | 1.03E-50 | -1.69E-46 |
| MS.gene059102 | MS.gene35212 | -0.811238 | 7.74E-51 | -1.69E-46 |
| MS.gene059102 | MS.gene35546 | 0.811987 | 5.32E-51 | -1.69E-46 |
| MS.gene059102 | MS.gene36594 | -0.800365 | 1.49E-48 | -1.69E-46 |
| MS.gene059102 | MS.gene37079 | -0.801125 | 1.04E-48 | -1.69E-46 |
| MS.gene059102 | MS.gene37487 | -0.810318 | 1.22E-50 | -1.69E-46 |
| MS.gene059102 | MS.gene38127 | -0.805708 | 1.17E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene38715 | -0.800795 | 1.22E-48 | -1.69E-46 |
| MS.gene059102 | MS.gene39520 | -0.808577 | 2.89E-50 | -1.69E-46 |
| MS.gene059102 | MS.gene40534 | -0.810854 | 9.37E-51 | -1.69E-46 |
| MS.gene059102 | MS.gene40958 | -0.817739 | 2.82E-52 | -1.69E-46 |
| MS.gene059102 | MS.gene41187 | -0.804756 | 1.85E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene42631 | -0.835095 | 2.05E-56 | -1.69E-46 |
| MS.gene059102 | MS.gene42636 | -0.847956 | 8.33E-60 | -1.69E-46 |
| MS.gene059102 | MS.gene42676 | -0.858741 | 6.69E-63 | -1.69E-46 |
| MS.gene059102 | MS.gene42678 | -0.812646 | 3.82E-51 | -1.69E-46 |
| MS.gene059102 | MS.gene46246 | -0.807443 | 5.04E-50 | -1.69E-46 |
| MS.gene059102 | MS.gene46300 | -0.822137 | 2.78E-53 | -1.69E-46 |
| MS.gene059102 | MS.gene46314 | -0.817653 | 2.95E-52 | -1.69E-46 |
| MS.gene059102 | MS.gene46895 | -0.810766 | 9.79E-51 | -1.69E-46 |
| MS.gene059102 | MS.gene49126 | -0.807773 | 4.29E-50 | -1.69E-46 |
| MS.gene059102 | MS.gene49716 | -0.810756 | 9.84E-51 | -1.69E-46 |
| MS.gene059102 | MS.gene49776 | -0.827254 | 1.73E-54 | -1.69E-46 |
| MS.gene059102 | MS.gene51605 | 0.802686 | 4.99E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene51837 | 0.834295 | 3.26E-56 | -1.69E-46 |
| MS.gene059102 | MS.gene52289 | -0.803122 | 4.05E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene52799 | -0.80872 | 2.70E-50 | -1.69E-46 |
| MS.gene059102 | MS.gene54119 | 0.828576 | 8.33E-55 | -1.69E-46 |
| MS.gene059102 | MS.gene54782 | -0.823172 | 1.60E-53 | -1.69E-46 |
| MS.gene059102 | MS.gene55694 | -0.823353 | 1.45E-53 | -1.69E-46 |
| MS.gene059102 | MS.gene55865 | -0.812126 | 4.96E-51 | -1.69E-46 |
| MS.gene059102 | MS.gene56456 | -0.842122 | 3.13E-58 | -1.69E-46 |
| MS.gene059102 | MS.gene56737 | -0.804138 | 2.49E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene58374 | -0.807681 | 4.49E-50 | -1.69E-46 |
| MS.gene059102 | MS.gene59555 | 0.819812 | 9.54E-53 | -1.69E-46 |
| MS.gene059102 | MS.gene60403 | -0.821114 | 4.80E-53 | -1.69E-46 |
| MS.gene059102 | MS.gene62792 | -0.819583 | 1.08E-52 | -1.69E-46 |
| MS.gene059102 | MS.gene62865 | -0.802989 | 4.32E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene65744 | -0.832461 | 9.33E-56 | -1.69E-46 |
| MS.gene059102 | MS.gene66305 | -0.8073 | 5.41E-50 | -1.69E-46 |
| MS.gene059102 | MS.gene67145 | 0.805724 | 1.16E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene68138 | -0.804189 | 2.43E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene68849 | 0.802736 | 4.87E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene68946 | 0.8179 | 2.59E-52 | -1.69E-46 |
| MS.gene059102 | MS.gene69357 | -0.801439 | 9.00E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene70106 | -0.822887 | 1.86E-53 | -1.69E-46 |
| MS.gene059102 | MS.gene70171 | -0.819624 | 1.05E-52 | -1.69E-46 |
| MS.gene059102 | MS.gene70692 | -0.811797 | 5.85E-51 | -1.69E-46 |
| MS.gene059102 | MS.gene71710 | -0.80587 | 1.08E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene71907 | -0.804366 | 2.24E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene72363 | -0.805315 | 1.42E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene73381 | -0.802033 | 6.80E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene73819 | -0.805411 | 1.35E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene75145 | -0.814981 | 1.17E-51 | -1.69E-46 |
| MS.gene059102 | MS.gene75561 | -0.825298 | 5.06E-54 | -1.69E-46 |
| MS.gene059102 | MS.gene76160 | 0.802983 | 4.33E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene77566 | -0.816738 | 4.74E-52 | -1.69E-46 |
| MS.gene059102 | MS.gene78193 | -0.827721 | 1.34E-54 | -1.69E-46 |
| MS.gene059102 | MS.gene82401 | -0.809104 | 2.23E-50 | -1.69E-46 |
| MS.gene059102 | MS.gene82403 | -0.804 | 2.67E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene85996 | 0.801121 | 1.05E-48 | -1.69E-46 |
| MS.gene059102 | MS.gene86725 | 0.863137 | 3.09E-64 | -1.69E-46 |
| MS.gene059102 | MS.gene88639 | -0.800195 | 1.62E-48 | -1.69E-46 |
| MS.gene059102 | MS.gene88666 | 0.801274 | 9.73E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene88851 | -0.818921 | 1.52E-52 | -1.69E-46 |
| MS.gene059102 | MS.gene90864 | -0.80987 | 1.53E-50 | -1.69E-46 |
| MS.gene059102 | MS.gene91963 | -0.802297 | 6.00E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene92366 | -0.828985 | 6.63E-55 | -1.69E-46 |
| MS.gene059102 | MS.gene93807 | 0.813695 | 2.25E-51 | -1.69E-46 |
| MS.gene059102 | MS.gene94547 | -0.810429 | 1.16E-50 | -1.69E-46 |
| MS.gene059102 | MS.gene94806 | -0.810389 | 1.18E-50 | -1.69E-46 |
| MS.gene059102 | MS.gene94829 | -0.810408 | 1.17E-50 | -1.69E-46 |
| MS.gene059102 | MS.gene95038 | -0.802481 | 5.50E-49 | -1.69E-46 |
| MS.gene059102 | MS.gene95272 | 0.801136 | 1.04E-48 | -1.69E-46 |
| MS.gene059102 | MS.gene97395 | -0.80929 | 2.04E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059102.t1 | MTR_1g009030 | 91.453 | 234 | 3 | 2 | 1 | 234 | 1 | 217 | 1.99e-155 | 430 |
| MS.gene059102.t1 | MTR_3g109280 | 83.772 | 228 | 37 | 0 | 1 | 228 | 1 | 228 | 1.85e-145 | 405 |
| MS.gene059102.t1 | MTR_1g077210 | 72.152 | 237 | 63 | 2 | 1 | 234 | 1 | 237 | 3.51e-125 | 354 |
| MS.gene059102.t1 | MTR_0054s0080 | 67.521 | 234 | 74 | 1 | 1 | 234 | 1 | 232 | 2.42e-116 | 332 |
| MS.gene059102.t1 | MTR_6g066040 | 58.772 | 228 | 92 | 1 | 1 | 228 | 1 | 226 | 2.63e-95 | 278 |
| MS.gene059102.t1 | MTR_6g066090 | 59.155 | 142 | 58 | 0 | 74 | 215 | 1 | 142 | 6.88e-56 | 176 |
| MS.gene059102.t1 | MTR_5g068640 | 37.719 | 228 | 135 | 2 | 5 | 226 | 3 | 229 | 7.91e-50 | 163 |
| MS.gene059102.t1 | MTR_8g106520 | 37.864 | 206 | 123 | 2 | 8 | 213 | 6 | 206 | 1.60e-40 | 139 |
| MS.gene059102.t1 | MTR_7g073370 | 38.889 | 216 | 129 | 1 | 7 | 222 | 6 | 218 | 5.34e-40 | 137 |
| MS.gene059102.t1 | MTR_8g106530 | 34.783 | 207 | 130 | 1 | 7 | 213 | 6 | 207 | 2.15e-34 | 129 |
| MS.gene059102.t1 | MTR_8g106530 | 30.460 | 174 | 113 | 3 | 40 | 213 | 297 | 462 | 8.16e-15 | 73.6 |
| MS.gene059102.t1 | MTR_2g014820 | 35.981 | 214 | 129 | 3 | 1 | 212 | 644 | 851 | 4.08e-33 | 126 |
| MS.gene059102.t1 | MTR_8g106520 | 37.013 | 154 | 92 | 2 | 60 | 213 | 2 | 150 | 1.76e-23 | 93.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059102.t1 | AT4G31330 | 69.456 | 239 | 64 | 2 | 1 | 233 | 1 | 236 | 8.37e-124 | 351 |
| MS.gene059102.t1 | AT5G10580 | 68.880 | 241 | 64 | 2 | 1 | 230 | 1 | 241 | 2.21e-121 | 345 |
| MS.gene059102.t1 | AT5G10580 | 67.623 | 244 | 68 | 2 | 1 | 233 | 1 | 244 | 2.47e-120 | 342 |
| MS.gene059102.t1 | AT5G10580 | 67.623 | 244 | 68 | 2 | 1 | 233 | 1 | 244 | 2.47e-120 | 342 |
| MS.gene059102.t1 | AT5G10580 | 70.270 | 185 | 47 | 1 | 1 | 177 | 1 | 185 | 7.94e-95 | 276 |
| MS.gene059102.t1 | AT5G24790 | 59.167 | 240 | 91 | 4 | 1 | 233 | 1 | 240 | 5.39e-90 | 265 |
| MS.gene059102.t1 | AT5G24600 | 35.417 | 240 | 140 | 3 | 4 | 231 | 2 | 238 | 1.03e-46 | 155 |
| MS.gene059102.t1 | AT5G24600 | 36.486 | 222 | 127 | 2 | 4 | 213 | 2 | 221 | 2.69e-45 | 151 |
| MS.gene059102.t1 | AT3G18215 | 36.493 | 211 | 134 | 0 | 3 | 213 | 2 | 212 | 1.07e-44 | 150 |
| MS.gene059102.t1 | AT5G43180 | 36.889 | 225 | 127 | 3 | 9 | 218 | 8 | 232 | 1.59e-36 | 129 |
| MS.gene059102.t1 | AT5G46060 | 33.649 | 211 | 136 | 2 | 4 | 213 | 6 | 213 | 5.51e-22 | 91.3 |
| MS.gene059102.t1 | AT3G18215 | 34.286 | 140 | 92 | 0 | 74 | 213 | 1 | 140 | 7.88e-20 | 83.6 |
Find 45 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAATATATATTTGAAATTT+TGG | 0.097168 | 1.2:+10942702 | MS.gene059102:CDS |
| CCACTAAATGATGCAATTTA+TGG | 0.137500 | 1.2:+10942526 | MS.gene059102:CDS |
| ATGCTAGTGGTCGTAGAAAT+TGG | 0.222133 | 1.2:+10941347 | MS.gene059102:CDS |
| CTGGACTTCCTTTGTTGCTT+TGG | 0.242448 | 1.2:+10942770 | MS.gene059102:CDS |
| AATTGAGGTTGTGGCCATTA+AGG | 0.286915 | 1.2:-10942449 | None:intergenic |
| ATGCTATTTGGATGTGATAT+TGG | 0.304552 | 1.2:+10941234 | MS.gene059102:CDS |
| GGAATGGAGGAAATGCTATT+TGG | 0.313682 | 1.2:+10941222 | MS.gene059102:CDS |
| TTAGCATTGGTTACCATGTT+TGG | 0.319355 | 1.2:+10941275 | MS.gene059102:CDS |
| ATCACTAAGGAACACAATAA+TGG | 0.320459 | 1.2:+10942420 | MS.gene059102:CDS |
| CCATAAATTGCATCATTTAG+TGG | 0.326808 | 1.2:-10942526 | None:intergenic |
| TTGGTTACCATGTTTGGCTT+TGG | 0.343248 | 1.2:+10941281 | MS.gene059102:CDS |
| CCATGTTTGGCTTTGGCATA+AGG | 0.345477 | 1.2:+10941288 | MS.gene059102:CDS |
| TCACTAAGGAACACAATAAT+GGG | 0.357357 | 1.2:+10942421 | MS.gene059102:CDS |
| GGATGTGATATTGGTTCCTT+TGG | 0.381322 | 1.2:+10941243 | MS.gene059102:CDS |
| GAGCAAAGGAAAACCATAAC+AGG | 0.393179 | 1.2:-10942802 | None:intergenic |
| AAAATTTCAAATATATATTC+TGG | 0.418167 | 1.2:-10942700 | None:intergenic |
| TGGAGCACATGGTGAATTCA+TGG | 0.428876 | 1.2:+10942546 | MS.gene059102:CDS |
| TTTCCTTTGCTCTCTTGCTA+TGG | 0.429928 | 1.2:+10942813 | MS.gene059102:CDS |
| CAGCCACACACAACCATTGT+TGG | 0.455340 | 1.2:+10941319 | MS.gene059102:CDS |
| TGCTAGTGGTCGTAGAAATT+GGG | 0.473756 | 1.2:+10941348 | MS.gene059102:CDS |
| TTTGTGTACACTAGTGGAAA+GGG | 0.482848 | 1.2:+10942859 | MS.gene059102:CDS |
| TTGGCAATGCTAATTAGCAT+TGG | 0.515120 | 1.2:+10941262 | MS.gene059102:CDS |
| AGTGACAAAAGAATGAGAAG+AGG | 0.517572 | 1.2:-10942603 | None:intergenic |
| ACATAGTAAAATTGAGGTTG+TGG | 0.520784 | 1.2:-10942458 | None:intergenic |
| GAATGTGAATGTGGATCAAA+AGG | 0.525644 | 1.2:+10942888 | MS.gene059102:CDS |
| ACCTGAACATAGTAAAATTG+AGG | 0.548897 | 1.2:-10942464 | None:intergenic |
| TTGGGTTAATGCTATGATGA+AGG | 0.554864 | 1.2:+10941366 | MS.gene059102:CDS |
| TCCTAGCTGTGCAATCACTA+AGG | 0.556695 | 1.2:+10942407 | MS.gene059102:CDS |
| CCTTATGCCAAAGCCAAACA+TGG | 0.572427 | 1.2:-10941288 | None:intergenic |
| CTGGTGTCACAAGTGACAAA+GGG | 0.574968 | 1.2:-10942681 | None:intergenic |
| AAATCAAGATTGTAAAGCAC+AGG | 0.577484 | 1.2:-10942838 | None:intergenic |
| ACAAGTGACAAAGGGTCTTG+AGG | 0.580606 | 1.2:-10942673 | None:intergenic |
| TCTGGTGTCACAAGTGACAA+AGG | 0.581114 | 1.2:-10942682 | None:intergenic |
| GAACCATAGCAAGAGAGCAA+AGG | 0.582292 | 1.2:-10942816 | None:intergenic |
| GGGAAAGATGAATGTGAATG+TGG | 0.592947 | 1.2:+10942879 | MS.gene059102:CDS |
| TCCTTAGTGATTGCACAGCT+AGG | 0.598866 | 1.2:-10942408 | None:intergenic |
| ATGAGAAGAGGAAAATTGTG+AGG | 0.605718 | 1.2:-10942591 | None:intergenic |
| TGTGAATGTGGATCAAAAGG+AGG | 0.642260 | 1.2:+10942891 | MS.gene059102:CDS |
| ATTGTTGGCATCAATGCTAG+TGG | 0.645700 | 1.2:+10941334 | MS.gene059102:CDS |
| CACTAAGGAACACAATAATG+GGG | 0.646915 | 1.2:+10942422 | MS.gene059102:CDS |
| ACTAGCATTGATGCCAACAA+TGG | 0.649656 | 1.2:-10941332 | None:intergenic |
| ATGCTAATTAGCATTGCCAA+AGG | 0.660900 | 1.2:-10941259 | None:intergenic |
| GATGCAATTTATGGAGCACA+TGG | 0.684664 | 1.2:+10942535 | MS.gene059102:CDS |
| ATGCCAACAATGGTTGTGTG+TGG | 0.695851 | 1.2:-10941322 | None:intergenic |
| ACTAAGGAACACAATAATGG+GGG | 0.700456 | 1.2:+10942423 | MS.gene059102:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTTCAAATATATATTC+TGG | - | chr1.2:10942703-10942722 | None:intergenic | 10.0% |
| !! | TAATATATCTTCAAAATAAT+AGG | + | chr1.2:10942259-10942278 | MS.gene059102:intron | 10.0% |
| !!! | AGAATATATATTTGAAATTT+TGG | + | chr1.2:10942702-10942721 | MS.gene059102:CDS | 10.0% |
| !!! | CATATATATAAGATTTTAAA+AGG | - | chr1.2:10942349-10942368 | None:intergenic | 10.0% |
| !! | CATAATGATAAAGATTTATA+GGG | - | chr1.2:10941435-10941454 | None:intergenic | 15.0% |
| !! | TAGATGAAAAGAAATATAAA+TGG | - | chr1.2:10941837-10941856 | None:intergenic | 15.0% |
| !! | TCATAATGATAAAGATTTAT+AGG | - | chr1.2:10941436-10941455 | None:intergenic | 15.0% |
| !!! | AGAAATCATATATTTTATGA+TGG | + | chr1.2:10941572-10941591 | MS.gene059102:intron | 15.0% |
| !!! | ATTTTTTTTTCATTTGAAGA+AGG | - | chr1.2:10941513-10941532 | None:intergenic | 15.0% |
| !!! | GTATTTTATTGATTTTATGA+GGG | + | chr1.2:10941594-10941613 | MS.gene059102:intron | 15.0% |
| !!! | TTTTTTATTACATTAGTTAG+TGG | - | chr1.2:10942020-10942039 | None:intergenic | 15.0% |
| !! | AATGGAATATGCAATATATA+TGG | - | chr1.2:10941819-10941838 | None:intergenic | 20.0% |
| !! | TCTTTCTAAATGGTATAATT+TGG | + | chr1.2:10941938-10941957 | MS.gene059102:intron | 20.0% |
| !! | TGTACGAATAAGAATAAATT+AGG | - | chr1.2:10941866-10941885 | None:intergenic | 20.0% |
| !!! | ATATTTGAAATTTTGGAGAA+AGG | + | chr1.2:10942709-10942728 | MS.gene059102:CDS | 20.0% |
| !!! | GGTATTTTATTGATTTTATG+AGG | + | chr1.2:10941593-10941612 | MS.gene059102:intron | 20.0% |
| !!! | TTTTTCACTATCAAATTGTT+AGG | + | chr1.2:10941730-10941749 | MS.gene059102:intron | 20.0% |
| ! | AACAGAATGTTCTTTCTAAA+TGG | + | chr1.2:10941928-10941947 | MS.gene059102:intron | 25.0% |
| ! | ATGTCCTCAAATATACTAAA+TGG | - | chr1.2:10941708-10941727 | None:intergenic | 25.0% |
| ! | GCTTAAATTCTTCAACTATT+TGG | + | chr1.2:10941674-10941693 | MS.gene059102:intron | 25.0% |
| ! | TATATGGTATAAACACTCTA+TGG | - | chr1.2:10941803-10941822 | None:intergenic | 25.0% |
| ! | TGTCCTCAAATATACTAAAT+GGG | - | chr1.2:10941707-10941726 | None:intergenic | 25.0% |
| !! | ACGTTATTTATGATGATGTT+AGG | + | chr1.2:10942316-10942335 | MS.gene059102:intron | 25.0% |
| !!! | TTTATGAGGGTATTTTATGA+TGG | + | chr1.2:10941607-10941626 | MS.gene059102:intron | 25.0% |
| AAATCAAGATTGTAAAGCAC+AGG | - | chr1.2:10942841-10942860 | None:intergenic | 30.0% | |
| ACATAGTAAAATTGAGGTTG+TGG | - | chr1.2:10942461-10942480 | None:intergenic | 30.0% | |
| ACCTGAACATAGTAAAATTG+AGG | - | chr1.2:10942467-10942486 | None:intergenic | 30.0% | |
| AGAGATTGAAAACTATGTCT+AGG | - | chr1.2:10942187-10942206 | None:intergenic | 30.0% | |
| ATCACTAAGGAACACAATAA+TGG | + | chr1.2:10942420-10942439 | MS.gene059102:CDS | 30.0% | |
| ATGATGTGTTTGATTATGAC+AGG | + | chr1.2:10942366-10942385 | MS.gene059102:intron | 30.0% | |
| CCACTAAATGATGCAATTTA+TGG | + | chr1.2:10942526-10942545 | MS.gene059102:CDS | 30.0% | |
| CCATAAATTGCATCATTTAG+TGG | - | chr1.2:10942529-10942548 | None:intergenic | 30.0% | |
| TAGCTTATCTTGAACATCAA+TGG | + | chr1.2:10941765-10941784 | MS.gene059102:intron | 30.0% | |
| TCAAAATAATAGGTACCATG+AGG | + | chr1.2:10942269-10942288 | MS.gene059102:intron | 30.0% | |
| TCACTAAGGAACACAATAAT+GGG | + | chr1.2:10942421-10942440 | MS.gene059102:CDS | 30.0% | |
| TGTCATAAGTTATAACCTCA+TGG | - | chr1.2:10942287-10942306 | None:intergenic | 30.0% | |
| TGTCCCATTTAGTATATTTG+AGG | + | chr1.2:10941701-10941720 | MS.gene059102:intron | 30.0% | |
| TTTGTACATGCACATTATCT+TGG | - | chr1.2:10941996-10942015 | None:intergenic | 30.0% | |
| ! | ACCTCAATTTTACTATGTTC+AGG | + | chr1.2:10942463-10942482 | MS.gene059102:CDS | 30.0% |
| ! | ATGCTATTTGGATGTGATAT+TGG | + | chr1.2:10941234-10941253 | MS.gene059102:CDS | 30.0% |
| ! | GTTTTGCTTTCTTTTGTGTT+TGG | - | chr1.2:10941911-10941930 | None:intergenic | 30.0% |
| ! | TTTTGAATGCAGTTGGAAAT+AGG | + | chr1.2:10942737-10942756 | MS.gene059102:CDS | 30.0% |
| ACTAAGGAACACAATAATGG+GGG | + | chr1.2:10942423-10942442 | MS.gene059102:CDS | 35.0% | |
| AGTGACAAAAGAATGAGAAG+AGG | - | chr1.2:10942606-10942625 | None:intergenic | 35.0% | |
| ATGAGAAGAGGAAAATTGTG+AGG | - | chr1.2:10942594-10942613 | None:intergenic | 35.0% | |
| ATGCTAATTAGCATTGCCAA+AGG | - | chr1.2:10941262-10941281 | None:intergenic | 35.0% | |
| CACTAAGGAACACAATAATG+GGG | + | chr1.2:10942422-10942441 | MS.gene059102:CDS | 35.0% | |
| CCAAAAATCCAAAGCAACAA+AGG | - | chr1.2:10942781-10942800 | None:intergenic | 35.0% | |
| GAATGTGAATGTGGATCAAA+AGG | + | chr1.2:10942888-10942907 | MS.gene059102:CDS | 35.0% | |
| TGAAAACTATGTCTAGGTAG+TGG | - | chr1.2:10942181-10942200 | None:intergenic | 35.0% | |
| TTGGCAATGCTAATTAGCAT+TGG | + | chr1.2:10941262-10941281 | MS.gene059102:CDS | 35.0% | |
| TTGGGTTAATGCTATGATGA+AGG | + | chr1.2:10941366-10941385 | MS.gene059102:CDS | 35.0% | |
| TTTGTGTACACTAGTGGAAA+GGG | + | chr1.2:10942859-10942878 | MS.gene059102:CDS | 35.0% | |
| ! | CTTGATTTTGTGTACACTAG+TGG | + | chr1.2:10942853-10942872 | MS.gene059102:CDS | 35.0% |
| ! | GGTTTCATTTTGAATGCAGT+TGG | + | chr1.2:10942730-10942749 | MS.gene059102:CDS | 35.0% |
| ! | TTCATTTGAAGAAGGTGTGA+GGG | - | chr1.2:10941505-10941524 | None:intergenic | 35.0% |
| ! | TTTCATTTGAAGAAGGTGTG+AGG | - | chr1.2:10941506-10941525 | None:intergenic | 35.0% |
| ! | TTTTGTGTACACTAGTGGAA+AGG | + | chr1.2:10942858-10942877 | MS.gene059102:CDS | 35.0% |
| !! | TTAGCATTGGTTACCATGTT+TGG | + | chr1.2:10941275-10941294 | MS.gene059102:CDS | 35.0% |
| !!! | CCTTTGTTGCTTTGGATTTT+TGG | + | chr1.2:10942778-10942797 | MS.gene059102:CDS | 35.0% |
| !!! | TTGGATTTTTGGTCCTGTTA+TGG | + | chr1.2:10942789-10942808 | MS.gene059102:CDS | 35.0% |
| AATTGAGGTTGTGGCCATTA+AGG | - | chr1.2:10942452-10942471 | None:intergenic | 40.0% | |
| ATGCTAGTGGTCGTAGAAAT+TGG | + | chr1.2:10941347-10941366 | MS.gene059102:CDS | 40.0% | |
| ATTGTTGGCATCAATGCTAG+TGG | + | chr1.2:10941334-10941353 | MS.gene059102:CDS | 40.0% | |
| GAGCAAAGGAAAACCATAAC+AGG | - | chr1.2:10942805-10942824 | None:intergenic | 40.0% | |
| GATGCAATTTATGGAGCACA+TGG | + | chr1.2:10942535-10942554 | MS.gene059102:CDS | 40.0% | |
| GGAATGGAGGAAATGCTATT+TGG | + | chr1.2:10941222-10941241 | MS.gene059102:CDS | 40.0% | |
| GGGAAAGATGAATGTGAATG+TGG | + | chr1.2:10942879-10942898 | MS.gene059102:CDS | 40.0% | |
| TGCTAGTGGTCGTAGAAATT+GGG | + | chr1.2:10941348-10941367 | MS.gene059102:CDS | 40.0% | |
| TGTGAATGTGGATCAAAAGG+AGG | + | chr1.2:10942891-10942910 | MS.gene059102:CDS | 40.0% | |
| TTTCCTTTGCTCTCTTGCTA+TGG | + | chr1.2:10942813-10942832 | MS.gene059102:CDS | 40.0% | |
| ! | AATAATGGGGGCAACCTTAA+TGG | + | chr1.2:10942435-10942454 | MS.gene059102:CDS | 40.0% |
| ! | GGAAATAGGCTTTTCTATGC+TGG | + | chr1.2:10942751-10942770 | MS.gene059102:CDS | 40.0% |
| ! | TCATTTGAAGAAGGTGTGAG+GGG | - | chr1.2:10941504-10941523 | None:intergenic | 40.0% |
| ! | TTGGTTACCATGTTTGGCTT+TGG | + | chr1.2:10941281-10941300 | MS.gene059102:CDS | 40.0% |
| !! | ACTAGCATTGATGCCAACAA+TGG | - | chr1.2:10941335-10941354 | None:intergenic | 40.0% |
| !! | GGATGTGATATTGGTTCCTT+TGG | + | chr1.2:10941243-10941262 | MS.gene059102:CDS | 40.0% |
| ACAAGTGACAAAGGGTCTTG+AGG | - | chr1.2:10942676-10942695 | None:intergenic | 45.0% | |
| ATGCCAACAATGGTTGTGTG+TGG | - | chr1.2:10941325-10941344 | None:intergenic | 45.0% | |
| CCTTATGCCAAAGCCAAACA+TGG | - | chr1.2:10941291-10941310 | None:intergenic | 45.0% | |
| GAACCATAGCAAGAGAGCAA+AGG | - | chr1.2:10942819-10942838 | None:intergenic | 45.0% | |
| GGTGAATATCCAATCCGAGT+GGG | + | chr1.2:10941959-10941978 | MS.gene059102:intron | 45.0% | |
| TAACAACAACCCACTCGGAT+TGG | - | chr1.2:10941971-10941990 | None:intergenic | 45.0% | |
| TCCTAGCTGTGCAATCACTA+AGG | + | chr1.2:10942407-10942426 | MS.gene059102:CDS | 45.0% | |
| TCCTTAGTGATTGCACAGCT+AGG | - | chr1.2:10942411-10942430 | None:intergenic | 45.0% | |
| TGGAGCACATGGTGAATTCA+TGG | + | chr1.2:10942546-10942565 | MS.gene059102:CDS | 45.0% | |
| TGGTCTAACAACAACCCACT+CGG | - | chr1.2:10941976-10941995 | None:intergenic | 45.0% | |
| TGGTGAATATCCAATCCGAG+TGG | + | chr1.2:10941958-10941977 | MS.gene059102:intron | 45.0% | |
| ! | CATTTGAAGAAGGTGTGAGG+GGG | - | chr1.2:10941503-10941522 | None:intergenic | 45.0% |
| ! | CTGGACTTCCTTTGTTGCTT+TGG | + | chr1.2:10942770-10942789 | MS.gene059102:CDS | 45.0% |
| !! | CCATGTTTGGCTTTGGCATA+AGG | + | chr1.2:10941288-10941307 | MS.gene059102:CDS | 45.0% |
| !! | CTGGTGTCACAAGTGACAAA+GGG | - | chr1.2:10942684-10942703 | None:intergenic | 45.0% |
| !! | TCTGGTGTCACAAGTGACAA+AGG | - | chr1.2:10942685-10942704 | None:intergenic | 45.0% |
| !! | AATGTAATAAAAAAAATTAA+AGG | + | chr1.2:10942026-10942045 | MS.gene059102:intron | 5.0% |
| !!! | ATATATATAAGATTTTAAAA+GGG | - | chr1.2:10942348-10942367 | None:intergenic | 5.0% |
| CAGCCACACACAACCATTGT+TGG | + | chr1.2:10941319-10941338 | MS.gene059102:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 10941220 | 10942924 | 10941220 | ID=MS.gene059102 |
| chr1.2 | mRNA | 10941220 | 10942924 | 10941220 | ID=MS.gene059102.t1;Parent=MS.gene059102 |
| chr1.2 | exon | 10941220 | 10941387 | 10941220 | ID=MS.gene059102.t1.exon1;Parent=MS.gene059102.t1 |
| chr1.2 | CDS | 10941220 | 10941387 | 10941220 | ID=cds.MS.gene059102.t1;Parent=MS.gene059102.t1 |
| chr1.2 | exon | 10942388 | 10942924 | 10942388 | ID=MS.gene059102.t1.exon2;Parent=MS.gene059102.t1 |
| chr1.2 | CDS | 10942388 | 10942924 | 10942388 | ID=cds.MS.gene059102.t1;Parent=MS.gene059102.t1 |
| Gene Sequence |
| Protein sequence |
