Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76302.t1 | XP_013470181.1 | 96.3 | 436 | 16 | 0 | 1 | 436 | 1 | 436 | 1.50E-235 | 825.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76302.t1 | Q8RWD0 | 45.3 | 446 | 192 | 12 | 6 | 434 | 3 | 413 | 2.9e-81 | 303.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76302.t1 | A0A072VQB9 | 96.3 | 436 | 16 | 0 | 1 | 436 | 1 | 436 | 1.1e-235 | 825.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene76302.t1 | TF | C2C2-CO-like |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049297 | MS.gene76302 | 0.802276 | 6.06E-49 | -1.69E-46 |
| MS.gene049346 | MS.gene76302 | 0.836655 | 8.24E-57 | -1.69E-46 |
| MS.gene049758 | MS.gene76302 | 0.825448 | 4.66E-54 | -1.69E-46 |
| MS.gene050099 | MS.gene76302 | 0.808895 | 2.47E-50 | -1.69E-46 |
| MS.gene050301 | MS.gene76302 | 0.825012 | 5.91E-54 | -1.69E-46 |
| MS.gene050330 | MS.gene76302 | -0.801 | 1.11E-48 | -1.69E-46 |
| MS.gene050397 | MS.gene76302 | 0.800086 | 1.70E-48 | -1.69E-46 |
| MS.gene050404 | MS.gene76302 | 0.837255 | 5.79E-57 | -1.69E-46 |
| MS.gene050406 | MS.gene76302 | 0.846604 | 1.96E-59 | -1.69E-46 |
| MS.gene050558 | MS.gene76302 | 0.809166 | 2.16E-50 | -1.69E-46 |
| MS.gene05101 | MS.gene76302 | 0.83451 | 2.87E-56 | -1.69E-46 |
| MS.gene051240 | MS.gene76302 | 0.802254 | 6.12E-49 | -1.69E-46 |
| MS.gene051471 | MS.gene76302 | 0.813993 | 1.93E-51 | -1.69E-46 |
| MS.gene051582 | MS.gene76302 | 0.802842 | 4.63E-49 | -1.69E-46 |
| MS.gene051662 | MS.gene76302 | 0.80229 | 6.02E-49 | -1.69E-46 |
| MS.gene052078 | MS.gene76302 | 0.802213 | 6.24E-49 | -1.69E-46 |
| MS.gene052178 | MS.gene76302 | 0.801733 | 7.83E-49 | -1.69E-46 |
| MS.gene052340 | MS.gene76302 | 0.804021 | 2.64E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene76302 | 0.854189 | 1.45E-61 | -1.69E-46 |
| MS.gene053907 | MS.gene76302 | 0.817334 | 3.48E-52 | -1.69E-46 |
| MS.gene053971 | MS.gene76302 | 0.802374 | 5.78E-49 | -1.69E-46 |
| MS.gene054473 | MS.gene76302 | 0.809864 | 1.53E-50 | -1.69E-46 |
| MS.gene054542 | MS.gene76302 | 0.803216 | 3.87E-49 | -1.69E-46 |
| MS.gene054942 | MS.gene76302 | 0.802594 | 5.21E-49 | -1.69E-46 |
| MS.gene054983 | MS.gene76302 | 0.821032 | 5.01E-53 | -1.69E-46 |
| MS.gene055422 | MS.gene76302 | 0.823552 | 1.30E-53 | -1.69E-46 |
| MS.gene055428 | MS.gene76302 | 0.838695 | 2.47E-57 | -1.69E-46 |
| MS.gene055712 | MS.gene76302 | 0.817712 | 2.86E-52 | -1.69E-46 |
| MS.gene056451 | MS.gene76302 | 0.83562 | 1.51E-56 | -1.69E-46 |
| MS.gene056612 | MS.gene76302 | 0.83145 | 1.66E-55 | -1.69E-46 |
| MS.gene05702 | MS.gene76302 | 0.841574 | 4.38E-58 | -1.69E-46 |
| MS.gene057121 | MS.gene76302 | 0.804633 | 1.97E-49 | -1.69E-46 |
| MS.gene057245 | MS.gene76302 | 0.813383 | 2.63E-51 | -1.69E-46 |
| MS.gene057439 | MS.gene76302 | 0.819786 | 9.67E-53 | -1.69E-46 |
| MS.gene058002 | MS.gene76302 | 0.822093 | 2.85E-53 | -1.69E-46 |
| MS.gene058276 | MS.gene76302 | 0.815756 | 7.85E-52 | -1.69E-46 |
| MS.gene05907 | MS.gene76302 | 0.805719 | 1.17E-49 | -1.69E-46 |
| MS.gene059228 | MS.gene76302 | 0.803771 | 2.97E-49 | -1.69E-46 |
| MS.gene059518 | MS.gene76302 | 0.800728 | 1.26E-48 | -1.69E-46 |
| MS.gene059894 | MS.gene76302 | 0.850425 | 1.71E-60 | -1.69E-46 |
| MS.gene061049 | MS.gene76302 | 0.859141 | 5.08E-63 | -1.69E-46 |
| MS.gene061112 | MS.gene76302 | 0.836137 | 1.12E-56 | -1.69E-46 |
| MS.gene061265 | MS.gene76302 | 0.832455 | 9.36E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76302.t1 | MTR_1g110870 | 96.330 | 436 | 16 | 0 | 1 | 436 | 1 | 436 | 0.0 | 874 |
| MS.gene76302.t1 | MTR_8g104190 | 41.593 | 452 | 187 | 18 | 9 | 433 | 11 | 412 | 3.46e-83 | 261 |
| MS.gene76302.t1 | MTR_5g072780 | 44.144 | 111 | 42 | 3 | 316 | 424 | 124 | 216 | 6.35e-19 | 85.5 |
| MS.gene76302.t1 | MTR_7g032240 | 40.152 | 132 | 56 | 4 | 316 | 424 | 220 | 351 | 5.67e-17 | 82.4 |
| MS.gene76302.t1 | MTR_4g127420 | 44.898 | 98 | 42 | 4 | 321 | 418 | 293 | 378 | 1.21e-13 | 72.4 |
| MS.gene76302.t1 | MTR_1g013450 | 47.561 | 82 | 36 | 2 | 351 | 427 | 221 | 300 | 2.27e-12 | 68.2 |
| MS.gene76302.t1 | MTR_3g105710 | 63.636 | 55 | 16 | 1 | 370 | 424 | 224 | 274 | 3.94e-12 | 67.0 |
| MS.gene76302.t1 | MTR_7g018170 | 69.767 | 43 | 13 | 0 | 382 | 424 | 327 | 369 | 8.70e-11 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76302.t1 | AT1G25440 | 46.313 | 434 | 185 | 11 | 6 | 424 | 3 | 403 | 1.21e-101 | 309 |
| MS.gene76302.t1 | AT1G68520 | 44.018 | 443 | 188 | 15 | 6 | 429 | 3 | 404 | 4.47e-92 | 284 |
| MS.gene76302.t1 | AT1G73870 | 38.303 | 436 | 199 | 18 | 8 | 427 | 9 | 390 | 2.35e-65 | 214 |
| MS.gene76302.t1 | AT4G25990 | 49.533 | 107 | 38 | 3 | 318 | 424 | 293 | 383 | 8.33e-22 | 97.1 |
| MS.gene76302.t1 | AT5G57180 | 45.217 | 115 | 43 | 3 | 318 | 431 | 337 | 432 | 2.63e-21 | 96.3 |
| MS.gene76302.t1 | AT1G49130 | 40.164 | 122 | 65 | 3 | 15 | 128 | 23 | 144 | 1.84e-19 | 89.4 |
| MS.gene76302.t1 | AT1G49130 | 45.794 | 107 | 37 | 3 | 319 | 425 | 236 | 321 | 7.66e-17 | 81.6 |
| MS.gene76302.t1 | AT1G49130 | 40.164 | 122 | 65 | 3 | 15 | 128 | 16 | 137 | 2.02e-19 | 89.0 |
| MS.gene76302.t1 | AT1G49130 | 45.794 | 107 | 37 | 2 | 319 | 425 | 229 | 314 | 1.00e-16 | 81.3 |
| MS.gene76302.t1 | AT4G25990 | 43.443 | 122 | 38 | 4 | 318 | 424 | 293 | 398 | 3.05e-18 | 86.7 |
| MS.gene76302.t1 | AT5G14370 | 38.028 | 142 | 73 | 4 | 291 | 424 | 202 | 336 | 4.55e-18 | 85.5 |
| MS.gene76302.t1 | AT4G25990 | 47.525 | 101 | 37 | 3 | 318 | 418 | 293 | 377 | 5.36e-18 | 85.9 |
| MS.gene76302.t1 | AT1G07050 | 43.750 | 128 | 45 | 5 | 303 | 426 | 91 | 195 | 1.95e-17 | 80.9 |
| MS.gene76302.t1 | AT5G57180 | 45.098 | 102 | 36 | 3 | 318 | 418 | 337 | 419 | 7.49e-17 | 82.8 |
| MS.gene76302.t1 | AT1G07050 | 41.667 | 120 | 43 | 5 | 303 | 418 | 91 | 187 | 4.21e-13 | 68.2 |
| MS.gene76302.t1 | AT5G24930 | 52.113 | 71 | 28 | 1 | 360 | 424 | 267 | 337 | 1.32e-11 | 66.2 |
| MS.gene76302.t1 | AT5G15840 | 52.308 | 65 | 28 | 2 | 1 | 62 | 41 | 105 | 8.18e-11 | 63.9 |
Find 105 sgRNAs with CRISPR-Local
Find 121 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAATCTCCCTTTCATTCT+TGG | 0.172964 | 1.1:+78261325 | None:intergenic |
| TCTCGGTGTTCTTGCCTTCT+TGG | 0.182825 | 1.1:+78262683 | None:intergenic |
| GAATAATTCATCCCCATTAA+TGG | 0.193260 | 1.1:+78262731 | None:intergenic |
| ATTATTTATCAGGGAACTTT+TGG | 0.202916 | 1.1:-78261517 | MS.gene76302:intron |
| TGAAAATAATGATTATAATA+AGG | 0.203891 | 1.1:-78262025 | MS.gene76302:CDS |
| TTCTCATCAGCATCCAAATT+TGG | 0.222169 | 1.1:+78261913 | None:intergenic |
| TTTCATCTTGTGCCGGATTT+AGG | 0.234999 | 1.1:-78262594 | MS.gene76302:CDS |
| TATGGTGAGTTTGGTGGATA+TGG | 0.250845 | 1.1:-78261475 | MS.gene76302:CDS |
| ATGAAGCGCAACTTCTTTAC+AGG | 0.254107 | 1.1:-78262536 | MS.gene76302:CDS |
| TAATGGTGACAAGCCAAATT+TGG | 0.271510 | 1.1:-78261926 | MS.gene76302:CDS |
| CTGCCTCCATCTACCGTTAC+TGG | 0.277791 | 1.1:+78261445 | None:intergenic |
| GAAAATAATGATTATAATAA+GGG | 0.279237 | 1.1:-78262024 | MS.gene76302:CDS |
| GCGTCGTACAAGTCCATTAA+TGG | 0.286147 | 1.1:-78262744 | MS.gene76302:CDS |
| ACGGAGGTTCATTATGCTTA+TGG | 0.290853 | 1.1:-78261493 | MS.gene76302:CDS |
| CTCAACATCAGCTGCAAATT+CGG | 0.302034 | 1.1:+78262302 | None:intergenic |
| GCAACAGAAGGATCGAAAAT+TGG | 0.331767 | 1.1:+78262510 | None:intergenic |
| TTCAATTGTGGTCCTTTCTC+TGG | 0.334946 | 1.1:-78262711 | MS.gene76302:CDS |
| CATTATGCTTATGGTGAGTT+TGG | 0.336402 | 1.1:-78261484 | MS.gene76302:CDS |
| GGTTGTTCTCAAAGAAAATA+AGG | 0.347288 | 1.1:-78261381 | MS.gene76302:CDS |
| GATTAGAAAATGAGTGTATA+GGG | 0.361641 | 1.1:-78262263 | MS.gene76302:CDS |
| CATTAGTCCAGGGACACTTT+TGG | 0.361781 | 1.1:+78261944 | None:intergenic |
| AGCTGATGTTGAGAGCTTAT+TGG | 0.374691 | 1.1:-78262292 | MS.gene76302:CDS |
| AGAAAATAAGGTACGAGGTT+AGG | 0.382846 | 1.1:-78261369 | MS.gene76302:CDS |
| GGATTAGAAAATGAGTGTAT+AGG | 0.385257 | 1.1:-78262264 | MS.gene76302:CDS |
| GCTGATGTTGAGAGCTTATT+GGG | 0.387062 | 1.1:-78262291 | MS.gene76302:CDS |
| CGTCGTACAAGTCCATTAAT+GGG | 0.391790 | 1.1:-78262743 | MS.gene76302:CDS |
| CAAAAGTGTCCCTGGACTAA+TGG | 0.395385 | 1.1:-78261943 | MS.gene76302:CDS |
| ATTCCTCGCTGGTTCGCTGC+TGG | 0.398768 | 1.1:+78262632 | None:intergenic |
| GAAGGCAAGAACACCGAGAC+AGG | 0.404794 | 1.1:-78262679 | MS.gene76302:CDS |
| TGTCATCCAGTAACGGTAGA+TGG | 0.408098 | 1.1:-78261451 | MS.gene76302:CDS |
| GGAGATAATATGATGGAGAT+TGG | 0.419606 | 1.1:-78262135 | MS.gene76302:CDS |
| GCAGAGAGGCAAGGGTTTCA+AGG | 0.431755 | 1.1:-78261426 | MS.gene76302:CDS |
| TGTATAGGGATGGAAGAGTT+GGG | 0.440723 | 1.1:-78262249 | MS.gene76302:CDS |
| ATAAATTATACCATGCAATC+AGG | 0.445629 | 1.1:+78261886 | None:intergenic |
| TGAAAGGGAGATTTGTGAAG+AGG | 0.460030 | 1.1:-78261318 | MS.gene76302:CDS |
| ATTTGGATGCTGATGAGAAC+TGG | 0.465263 | 1.1:-78261909 | MS.gene76302:CDS |
| GTGTATAGGGATGGAAGAGT+TGG | 0.474392 | 1.1:-78262250 | MS.gene76302:CDS |
| TGGCTTGTCACCATTAGTCC+AGG | 0.479305 | 1.1:+78261933 | None:intergenic |
| TACCGCTCCTAGTCCTCCTT+CGG | 0.479739 | 1.1:+78262452 | None:intergenic |
| AAATCCGGCACAAGATGAAA+CGG | 0.483022 | 1.1:+78262597 | None:intergenic |
| AGCGGAAAGGTGAAGGAAGA+AGG | 0.486561 | 1.1:-78262183 | MS.gene76302:CDS |
| GTTGAGAGCTTATTGGGTAG+GGG | 0.493508 | 1.1:-78262285 | MS.gene76302:CDS |
| TGTACTAGTCCTCCTTCAGT+TGG | 0.493939 | 1.1:-78262480 | MS.gene76302:CDS |
| CTGTTGGAGGCAAAACTGCT+AGG | 0.494037 | 1.1:-78262905 | MS.gene76302:CDS |
| TGTTGAGAGCTTATTGGGTA+GGG | 0.498854 | 1.1:-78262286 | MS.gene76302:CDS |
| GGTTCAACCGAAGGAGGACT+AGG | 0.500918 | 1.1:-78262459 | MS.gene76302:CDS |
| GAAGTGTAGCGGAAAGGTGA+AGG | 0.508915 | 1.1:-78262190 | MS.gene76302:CDS |
| TATGCTTATGGTGAGTTTGG+TGG | 0.509644 | 1.1:-78261481 | MS.gene76302:CDS |
| AGAGAGCTATGAAATTGTTG+AGG | 0.512413 | 1.1:-78262157 | MS.gene76302:CDS |
| AGATGGAGGCAGAGAGGCAA+GGG | 0.513332 | 1.1:-78261434 | MS.gene76302:CDS |
| GAAAGGGAGATTTGTGAAGA+GGG | 0.514753 | 1.1:-78261317 | MS.gene76302:CDS |
| GAGAAGAGACCAAGAATGAA+AGG | 0.514881 | 1.1:-78261334 | MS.gene76302:CDS |
| TTTCTCTGGCTTCACCAAGA+AGG | 0.516586 | 1.1:-78262697 | MS.gene76302:CDS |
| ATATTATTATTATTCCTCGC+TGG | 0.519712 | 1.1:+78262621 | None:intergenic |
| GAGAGCTATGAAATTGTTGA+GGG | 0.520749 | 1.1:-78262156 | MS.gene76302:CDS |
| CAGCTAATTCTTTAGCTCGA+AGG | 0.521112 | 1.1:-78262791 | MS.gene76302:CDS |
| GGGATAGCCAAAAGTGTCCC+TGG | 0.521182 | 1.1:-78261951 | MS.gene76302:CDS |
| AATCCGGCACAAGATGAAAC+GGG | 0.522679 | 1.1:+78262598 | None:intergenic |
| ATGTTGAGAGCTTATTGGGT+AGG | 0.523810 | 1.1:-78262287 | MS.gene76302:CDS |
| GATTTGTGTTTCCCCTGTCT+CGG | 0.527088 | 1.1:+78262666 | None:intergenic |
| CCTTCAGTTGGTTCAACCGA+AGG | 0.529802 | 1.1:-78262468 | MS.gene76302:CDS |
| ACTTCATCGAAACCTAAATC+CGG | 0.535576 | 1.1:+78262582 | None:intergenic |
| TTCTTGGTGAAGCCAGAGAA+AGG | 0.539259 | 1.1:+78262699 | None:intergenic |
| GGGGATGAATTATTCAATTG+TGG | 0.543849 | 1.1:-78262723 | MS.gene76302:CDS |
| AGTGAGAAGTGGAAGTGTAG+CGG | 0.549448 | 1.1:-78262201 | MS.gene76302:CDS |
| TGAAACACTTGAAGAGGTGA+AGG | 0.552820 | 1.1:-78262067 | MS.gene76302:CDS |
| AACGTAGCAAACGCTGTTGG+AGG | 0.555203 | 1.1:-78262918 | MS.gene76302:CDS |
| GAAGAGGTGAAGGAGAAGTG+TGG | 0.556028 | 1.1:-78262057 | MS.gene76302:CDS |
| CCTCGCTGGTTCGCTGCTGG+AGG | 0.557584 | 1.1:+78262635 | None:intergenic |
| TTCTCATGAAACACTTGAAG+AGG | 0.559028 | 1.1:-78262073 | MS.gene76302:CDS |
| ATGATGCAGTAATCATTGCA+TGG | 0.567325 | 1.1:-78261972 | MS.gene76302:CDS |
| TCGGCTAATTCAATATCAGA+TGG | 0.569236 | 1.1:+78262321 | None:intergenic |
| AGAAGAGACCAAGAATGAAA+GGG | 0.570209 | 1.1:-78261333 | MS.gene76302:CDS |
| GAAGTGGAAGTGTAGCGGAA+AGG | 0.571795 | 1.1:-78262196 | MS.gene76302:CDS |
| TGATGCAGTAATCATTGCAT+GGG | 0.576146 | 1.1:-78261971 | MS.gene76302:CDS |
| TAGATGGAGGCAGAGAGGCA+AGG | 0.578587 | 1.1:-78261435 | MS.gene76302:CDS |
| GGCTTGTCACCATTAGTCCA+GGG | 0.579867 | 1.1:+78261934 | None:intergenic |
| TGTTGAGGGAGATAATATGA+TGG | 0.580179 | 1.1:-78262142 | MS.gene76302:CDS |
| AAAATAATGATTATAATAAG+GGG | 0.580910 | 1.1:-78262023 | MS.gene76302:CDS |
| TGTTGGAGGCAAAACTGCTA+GGG | 0.581207 | 1.1:-78262904 | MS.gene76302:CDS |
| TATCCCGTTTCATCTTGTGC+CGG | 0.584262 | 1.1:-78262601 | MS.gene76302:CDS |
| AGAAAATGAGTGTATAGGGA+TGG | 0.586862 | 1.1:-78262259 | MS.gene76302:CDS |
| TAGTGATAATAATTATGAGA+TGG | 0.589411 | 1.1:-78262364 | MS.gene76302:CDS |
| TCAGTTGGTTCAACCGAAGG+AGG | 0.592160 | 1.1:-78262465 | MS.gene76302:CDS |
| AACATGAGGAGAGTGAGAAG+TGG | 0.595708 | 1.1:-78262212 | MS.gene76302:CDS |
| AGGAAATGTTGGAACTGCAA+AGG | 0.601959 | 1.1:+78261291 | None:intergenic |
| CCTTCGGTTGAACCAACTGA+AGG | 0.605624 | 1.1:+78262468 | None:intergenic |
| TGAGAACTGGCCTGATTGCA+TGG | 0.605682 | 1.1:-78261896 | MS.gene76302:intron |
| AGTGAAAGCAAAGTTCAACT+AGG | 0.611161 | 1.1:-78262387 | MS.gene76302:CDS |
| TCGGTTGAACCAACTGAAGG+AGG | 0.611596 | 1.1:+78262471 | None:intergenic |
| AGGCAAGAACACCGAGACAG+GGG | 0.619152 | 1.1:-78262677 | MS.gene76302:CDS |
| AAGGCAAGAACACCGAGACA+GGG | 0.629239 | 1.1:-78262678 | MS.gene76302:CDS |
| TGAAGCGCAACTTCTTTACA+GGG | 0.630911 | 1.1:-78262535 | MS.gene76302:CDS |
| GTACATAATTCAGCAACAGA+AGG | 0.631423 | 1.1:+78262498 | None:intergenic |
| CTCAAAGAAAATAAGGTACG+AGG | 0.632554 | 1.1:-78261374 | MS.gene76302:CDS |
| ATTGATTGATACAAAACATG+AGG | 0.635964 | 1.1:-78262226 | MS.gene76302:CDS |
| AACGGTAGATGGAGGCAGAG+AGG | 0.643178 | 1.1:-78261440 | MS.gene76302:CDS |
| ACAGAGAAAAGCGAAGGACA+AGG | 0.644471 | 1.1:-78261402 | MS.gene76302:CDS |
| CAAGGTACAGAGAAAAGCGA+AGG | 0.648816 | 1.1:-78261408 | MS.gene76302:CDS |
| ATATGGATGTCATCCAGTAA+CGG | 0.662808 | 1.1:-78261458 | MS.gene76302:CDS |
| CATCCAGTAACGGTAGATGG+AGG | 0.676181 | 1.1:-78261448 | MS.gene76302:CDS |
| GTCGTACAAGTCCATTAATG+GGG | 0.678758 | 1.1:-78262742 | MS.gene76302:CDS |
| AACCGAAGGAGGACTAGGAG+CGG | 0.681167 | 1.1:-78262454 | MS.gene76302:CDS |
| CCTCCAGCAGCGAACCAGCG+AGG | 0.699815 | 1.1:-78262635 | MS.gene76302:CDS |
| GCATAACAAAGAGAGCACGT+TGG | 0.777160 | 1.1:-78262869 | MS.gene76302:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATAATGATTATAATAAG+GGG | - | chr1.1:78262192-78262211 | MS.gene76302:CDS | 10.0% |
| !! | GAAAATAATGATTATAATAA+GGG | - | chr1.1:78262191-78262210 | MS.gene76302:CDS | 10.0% |
| !! | TGAAAATAATGATTATAATA+AGG | - | chr1.1:78262190-78262209 | MS.gene76302:CDS | 10.0% |
| !! | GAAATATCTAAATGTATATT+TGG | + | chr1.1:78262472-78262491 | None:intergenic | 15.0% |
| !!! | CTTTCTATCAAATTTTATAA+AGG | - | chr1.1:78262635-78262654 | MS.gene76302:CDS | 15.0% |
| !!! | TATATCTTAATTTTTCTTCA+TGG | - | chr1.1:78262349-78262368 | MS.gene76302:CDS | 15.0% |
| !! | ATTTGATAGAAAGAAAAAAG+TGG | + | chr1.1:78262628-78262647 | None:intergenic | 20.0% |
| !! | TAGTGATAATAATTATGAGA+TGG | - | chr1.1:78261851-78261870 | MS.gene76302:intron | 20.0% |
| !! | TTTACTGTTAGATTAATCAT+TGG | - | chr1.1:78262602-78262621 | MS.gene76302:CDS | 20.0% |
| ! | ATAAATTATACCATGCAATC+AGG | + | chr1.1:78262332-78262351 | None:intergenic | 25.0% |
| ! | ATATTATTATTATTCCTCGC+TGG | + | chr1.1:78261597-78261616 | None:intergenic | 25.0% |
| ! | ATTGATTGATACAAAACATG+AGG | - | chr1.1:78261989-78262008 | MS.gene76302:CDS | 25.0% |
| ! | CAATCTCTTTAGTTAATCAT+TGG | - | chr1.1:78262387-78262406 | MS.gene76302:CDS | 25.0% |
| ! | GATTAGAAAATGAGTGTATA+GGG | - | chr1.1:78261952-78261971 | MS.gene76302:CDS | 25.0% |
| !! | ATTATTTATCAGGGAACTTT+TGG | - | chr1.1:78262698-78262717 | MS.gene76302:CDS | 25.0% |
| !!! | TATTTTAGCAAAGGAAATGT+TGG | + | chr1.1:78262938-78262957 | None:intergenic | 25.0% |
| GAATAATTCATCCCCATTAA+TGG | + | chr1.1:78261487-78261506 | None:intergenic | 30.0% | |
| GGATTAGAAAATGAGTGTAT+AGG | - | chr1.1:78261951-78261970 | MS.gene76302:CDS | 30.0% | |
| GGTTGTTCTCAAAGAAAATA+AGG | - | chr1.1:78262834-78262853 | MS.gene76302:CDS | 30.0% | |
| !!! | CTCTCATTGTTTTTAACATC+AGG | + | chr1.1:78261810-78261829 | None:intergenic | 30.0% |
| ACAAATCTCCCTTTCATTCT+TGG | + | chr1.1:78262893-78262912 | None:intergenic | 35.0% | |
| ACAGTAAAATAGTGAACAGC+AGG | + | chr1.1:78262590-78262609 | None:intergenic | 35.0% | |
| ACTTCATCGAAACCTAAATC+CGG | + | chr1.1:78261636-78261655 | None:intergenic | 35.0% | |
| AGAAAATAAGGTACGAGGTT+AGG | - | chr1.1:78262846-78262865 | MS.gene76302:CDS | 35.0% | |
| AGAAAATGAGTGTATAGGGA+TGG | - | chr1.1:78261956-78261975 | MS.gene76302:CDS | 35.0% | |
| AGAAGAGACCAAGAATGAAA+GGG | - | chr1.1:78262882-78262901 | MS.gene76302:CDS | 35.0% | |
| AGAGAGCTATGAAATTGTTG+AGG | - | chr1.1:78262058-78262077 | MS.gene76302:CDS | 35.0% | |
| AGTGAAAGCAAAGTTCAACT+AGG | - | chr1.1:78261828-78261847 | MS.gene76302:intron | 35.0% | |
| ATATGGATGTCATCCAGTAA+CGG | - | chr1.1:78262757-78262776 | MS.gene76302:CDS | 35.0% | |
| ATGATGCAGTAATCATTGCA+TGG | - | chr1.1:78262243-78262262 | MS.gene76302:CDS | 35.0% | |
| CATTATGCTTATGGTGAGTT+TGG | - | chr1.1:78262731-78262750 | MS.gene76302:CDS | 35.0% | |
| CTCAAAGAAAATAAGGTACG+AGG | - | chr1.1:78262841-78262860 | MS.gene76302:CDS | 35.0% | |
| GAGAGCTATGAAATTGTTGA+GGG | - | chr1.1:78262059-78262078 | MS.gene76302:CDS | 35.0% | |
| GGGGATGAATTATTCAATTG+TGG | - | chr1.1:78261492-78261511 | MS.gene76302:CDS | 35.0% | |
| GTACATAATTCAGCAACAGA+AGG | + | chr1.1:78261720-78261739 | None:intergenic | 35.0% | |
| TAATGGTGACAAGCCAAATT+TGG | - | chr1.1:78262289-78262308 | MS.gene76302:CDS | 35.0% | |
| TCGGCTAATTCAATATCAGA+TGG | + | chr1.1:78261897-78261916 | None:intergenic | 35.0% | |
| TGATGCAGTAATCATTGCAT+GGG | - | chr1.1:78262244-78262263 | MS.gene76302:CDS | 35.0% | |
| TGTTGAGGGAGATAATATGA+TGG | - | chr1.1:78262073-78262092 | MS.gene76302:CDS | 35.0% | |
| TTCTCATCAGCATCCAAATT+TGG | + | chr1.1:78262305-78262324 | None:intergenic | 35.0% | |
| TTCTCATGAAACACTTGAAG+AGG | - | chr1.1:78262142-78262161 | MS.gene76302:CDS | 35.0% | |
| ! | GGAGATAATATGATGGAGAT+TGG | - | chr1.1:78262080-78262099 | MS.gene76302:CDS | 35.0% |
| !! | TTATCAGGGAACTTTTGGAA+CGG | - | chr1.1:78262703-78262722 | MS.gene76302:CDS | 35.0% |
| AAAAACGTAGCAAACGCTGT+TGG | - | chr1.1:78261294-78261313 | MS.gene76302:CDS | 40.0% | |
| AAATCCGGCACAAGATGAAA+CGG | + | chr1.1:78261621-78261640 | None:intergenic | 40.0% | |
| ACGGAGGTTCATTATGCTTA+TGG | - | chr1.1:78262722-78262741 | MS.gene76302:CDS | 40.0% | |
| AGCTGATGTTGAGAGCTTAT+TGG | - | chr1.1:78261923-78261942 | MS.gene76302:CDS | 40.0% | |
| AGGAAATGTTGGAACTGCAA+AGG | + | chr1.1:78262927-78262946 | None:intergenic | 40.0% | |
| ATGAAGCGCAACTTCTTTAC+AGG | - | chr1.1:78261679-78261698 | MS.gene76302:intron | 40.0% | |
| ATGTTGAGAGCTTATTGGGT+AGG | - | chr1.1:78261928-78261947 | MS.gene76302:CDS | 40.0% | |
| ATTTGGATGCTGATGAGAAC+TGG | - | chr1.1:78262306-78262325 | MS.gene76302:CDS | 40.0% | |
| CGTCGTACAAGTCCATTAAT+GGG | - | chr1.1:78261472-78261491 | MS.gene76302:CDS | 40.0% | |
| CTCAACATCAGCTGCAAATT+CGG | + | chr1.1:78261916-78261935 | None:intergenic | 40.0% | |
| CTTTACAACACTTGCACACA+CGG | - | chr1.1:78262541-78262560 | MS.gene76302:CDS | 40.0% | |
| GAAAGGGAGATTTGTGAAGA+GGG | - | chr1.1:78262898-78262917 | MS.gene76302:CDS | 40.0% | |
| GAGAAGAGACCAAGAATGAA+AGG | - | chr1.1:78262881-78262900 | MS.gene76302:CDS | 40.0% | |
| GCAACAGAAGGATCGAAAAT+TGG | + | chr1.1:78261708-78261727 | None:intergenic | 40.0% | |
| GCTGATGTTGAGAGCTTATT+GGG | - | chr1.1:78261924-78261943 | MS.gene76302:CDS | 40.0% | |
| GTCGTACAAGTCCATTAATG+GGG | - | chr1.1:78261473-78261492 | MS.gene76302:CDS | 40.0% | |
| TATGCTTATGGTGAGTTTGG+TGG | - | chr1.1:78262734-78262753 | MS.gene76302:CDS | 40.0% | |
| TGAAACACTTGAAGAGGTGA+AGG | - | chr1.1:78262148-78262167 | MS.gene76302:CDS | 40.0% | |
| TGAAAGGGAGATTTGTGAAG+AGG | - | chr1.1:78262897-78262916 | MS.gene76302:CDS | 40.0% | |
| TGAAGCGCAACTTCTTTACA+GGG | - | chr1.1:78261680-78261699 | MS.gene76302:intron | 40.0% | |
| TGTATAGGGATGGAAGAGTT+GGG | - | chr1.1:78261966-78261985 | MS.gene76302:CDS | 40.0% | |
| TGTTGAGAGCTTATTGGGTA+GGG | - | chr1.1:78261929-78261948 | MS.gene76302:CDS | 40.0% | |
| TTCAATTGTGGTCCTTTCTC+TGG | - | chr1.1:78261504-78261523 | MS.gene76302:CDS | 40.0% | |
| TTTCATCTTGTGCCGGATTT+AGG | - | chr1.1:78261621-78261640 | MS.gene76302:intron | 40.0% | |
| ! | CAGCTAATTCTTTAGCTCGA+AGG | - | chr1.1:78261424-78261443 | MS.gene76302:CDS | 40.0% |
| ! | TATGGTGAGTTTGGTGGATA+TGG | - | chr1.1:78262740-78262759 | MS.gene76302:CDS | 40.0% |
| !! | TCGTGATTTTGACACACTCA+AGG | - | chr1.1:78262441-78262460 | MS.gene76302:CDS | 40.0% |
| AACATGAGGAGAGTGAGAAG+TGG | - | chr1.1:78262003-78262022 | MS.gene76302:CDS | 45.0% | |
| AATCCGGCACAAGATGAAAC+GGG | + | chr1.1:78261620-78261639 | None:intergenic | 45.0% | |
| ACAGAGAAAAGCGAAGGACA+AGG | - | chr1.1:78262813-78262832 | MS.gene76302:CDS | 45.0% | |
| CAAGGTACAGAGAAAAGCGA+AGG | - | chr1.1:78262807-78262826 | MS.gene76302:CDS | 45.0% | |
| GATTTGTGTTTCCCCTGTCT+CGG | + | chr1.1:78261552-78261571 | None:intergenic | 45.0% | |
| GCGTCGTACAAGTCCATTAA+TGG | - | chr1.1:78261471-78261490 | MS.gene76302:CDS | 45.0% | |
| GTGTATAGGGATGGAAGAGT+TGG | - | chr1.1:78261965-78261984 | MS.gene76302:CDS | 45.0% | |
| GTTGAGAGCTTATTGGGTAG+GGG | - | chr1.1:78261930-78261949 | MS.gene76302:CDS | 45.0% | |
| TATCCCGTTTCATCTTGTGC+CGG | - | chr1.1:78261614-78261633 | MS.gene76302:intron | 45.0% | |
| TGTACTAGTCCTCCTTCAGT+TGG | - | chr1.1:78261735-78261754 | MS.gene76302:intron | 45.0% | |
| TGTCATCCAGTAACGGTAGA+TGG | - | chr1.1:78262764-78262783 | MS.gene76302:CDS | 45.0% | |
| TGTTGGAGGCAAAACTGCTA+GGG | - | chr1.1:78261311-78261330 | MS.gene76302:CDS | 45.0% | |
| TTCTTGGTGAAGCCAGAGAA+AGG | + | chr1.1:78261519-78261538 | None:intergenic | 45.0% | |
| ! | AGTGAGAAGTGGAAGTGTAG+CGG | - | chr1.1:78262014-78262033 | MS.gene76302:CDS | 45.0% |
| ! | CATTAGTCCAGGGACACTTT+TGG | + | chr1.1:78262274-78262293 | None:intergenic | 45.0% |
| ! | GCATAACAAAGAGAGCACGT+TGG | - | chr1.1:78261346-78261365 | MS.gene76302:CDS | 45.0% |
| ! | TTTCTCTGGCTTCACCAAGA+AGG | - | chr1.1:78261518-78261537 | MS.gene76302:intron | 45.0% |
| !! | CAAAAGTGTCCCTGGACTAA+TGG | - | chr1.1:78262272-78262291 | MS.gene76302:CDS | 45.0% |
| !!! | TTTAATTTAATTATTTATCA+GGG | - | chr1.1:78262689-78262708 | MS.gene76302:CDS | 5.0% |
| !!! | TTTTAATTTAATTATTTATC+AGG | - | chr1.1:78262688-78262707 | MS.gene76302:CDS | 5.0% |
| AACGTAGCAAACGCTGTTGG+AGG | - | chr1.1:78261297-78261316 | MS.gene76302:CDS | 50.0% | |
| AAGGCAAGAACACCGAGACA+GGG | - | chr1.1:78261537-78261556 | MS.gene76302:intron | 50.0% | |
| AGCGGAAAGGTGAAGGAAGA+AGG | - | chr1.1:78262032-78262051 | MS.gene76302:CDS | 50.0% | |
| CATCCAGTAACGGTAGATGG+AGG | - | chr1.1:78262767-78262786 | MS.gene76302:CDS | 50.0% | |
| CCTTCGGTTGAACCAACTGA+AGG | + | chr1.1:78261750-78261769 | None:intergenic | 50.0% | |
| CTGTTGGAGGCAAAACTGCT+AGG | - | chr1.1:78261310-78261329 | MS.gene76302:CDS | 50.0% | |
| GAAGAGGTGAAGGAGAAGTG+TGG | - | chr1.1:78262158-78262177 | MS.gene76302:CDS | 50.0% | |
| GGCTTGTCACCATTAGTCCA+GGG | + | chr1.1:78262284-78262303 | None:intergenic | 50.0% | |
| TCGGTTGAACCAACTGAAGG+AGG | + | chr1.1:78261747-78261766 | None:intergenic | 50.0% | |
| ! | CCTTCAGTTGGTTCAACCGA+AGG | - | chr1.1:78261747-78261766 | MS.gene76302:intron | 50.0% |
| ! | GAAGTGGAAGTGTAGCGGAA+AGG | - | chr1.1:78262019-78262038 | MS.gene76302:CDS | 50.0% |
| ! | GAAGTGTAGCGGAAAGGTGA+AGG | - | chr1.1:78262025-78262044 | MS.gene76302:CDS | 50.0% |
| ! | TCAGTTGGTTCAACCGAAGG+AGG | - | chr1.1:78261750-78261769 | MS.gene76302:intron | 50.0% |
| ! | TGAGAACTGGCCTGATTGCA+TGG | - | chr1.1:78262319-78262338 | MS.gene76302:CDS | 50.0% |
| ! | TGGCTTGTCACCATTAGTCC+AGG | + | chr1.1:78262285-78262304 | None:intergenic | 50.0% |
| !! | TCTCGGTGTTCTTGCCTTCT+TGG | + | chr1.1:78261535-78261554 | None:intergenic | 50.0% |
| !!! | TCAGGGAACTTTTGGAACGG+AGG | - | chr1.1:78262706-78262725 | MS.gene76302:CDS | 50.0% |
| AACCGAAGGAGGACTAGGAG+CGG | - | chr1.1:78261761-78261780 | MS.gene76302:intron | 55.0% | |
| AACGGTAGATGGAGGCAGAG+AGG | - | chr1.1:78262775-78262794 | MS.gene76302:CDS | 55.0% | |
| AGATGGAGGCAGAGAGGCAA+GGG | - | chr1.1:78262781-78262800 | MS.gene76302:CDS | 55.0% | |
| AGGCAAGAACACCGAGACAG+GGG | - | chr1.1:78261538-78261557 | MS.gene76302:intron | 55.0% | |
| CTGCCTCCATCTACCGTTAC+TGG | + | chr1.1:78262773-78262792 | None:intergenic | 55.0% | |
| GAAGGCAAGAACACCGAGAC+AGG | - | chr1.1:78261536-78261555 | MS.gene76302:intron | 55.0% | |
| GCAGAGAGGCAAGGGTTTCA+AGG | - | chr1.1:78262789-78262808 | MS.gene76302:CDS | 55.0% | |
| GGTTCAACCGAAGGAGGACT+AGG | - | chr1.1:78261756-78261775 | MS.gene76302:intron | 55.0% | |
| TACCGCTCCTAGTCCTCCTT+CGG | + | chr1.1:78261766-78261785 | None:intergenic | 55.0% | |
| TAGATGGAGGCAGAGAGGCA+AGG | - | chr1.1:78262780-78262799 | MS.gene76302:CDS | 55.0% | |
| !! | GGGATAGCCAAAAGTGTCCC+TGG | - | chr1.1:78262264-78262283 | MS.gene76302:CDS | 55.0% |
| !! | ATTCCTCGCTGGTTCGCTGC+TGG | + | chr1.1:78261586-78261605 | None:intergenic | 60.0% |
| CCTCCAGCAGCGAACCAGCG+AGG | - | chr1.1:78261580-78261599 | MS.gene76302:intron | 70.0% | |
| !! | CCTCGCTGGTTCGCTGCTGG+AGG | + | chr1.1:78261583-78261602 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 78261279 | 78262958 | 78261279 | ID=MS.gene76302 |
| chr1.1 | mRNA | 78261279 | 78262958 | 78261279 | ID=MS.gene76302.t1;Parent=MS.gene76302 |
| chr1.1 | exon | 78261897 | 78262958 | 78261897 | ID=MS.gene76302.t1.exon1;Parent=MS.gene76302.t1 |
| chr1.1 | CDS | 78261897 | 78262958 | 78261897 | ID=cds.MS.gene76302.t1;Parent=MS.gene76302.t1 |
| chr1.1 | exon | 78261279 | 78261527 | 78261279 | ID=MS.gene76302.t1.exon2;Parent=MS.gene76302.t1 |
| chr1.1 | CDS | 78261279 | 78261527 | 78261279 | ID=cds.MS.gene76302.t1;Parent=MS.gene76302.t1 |
| Gene Sequence |
| Protein sequence |
