AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77173


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77173.t1 MTR_2g061450 92.565 269 20 0 1 269 164 432 0.0 506
MS.gene77173.t1 MTR_2g060620 86.486 111 13 1 139 249 61 169 1.08e-60 191
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77173.t1 AT1G74120 57.249 269 115 0 1 269 177 445 2.34e-110 326
MS.gene77173.t1 AT5G54180 29.730 185 125 4 10 191 290 472 2.56e-13 69.7
MS.gene77173.t1 AT1G62010 32.787 122 79 3 101 220 268 388 4.83e-13 68.9
MS.gene77173.t1 AT1G62120 29.167 120 84 1 102 220 291 410 8.51e-11 62.4

Find 64 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTAGGGATCCAAGGATTT+TGG 0.152438 2.4:+31789755 MS.gene77173:CDS
TGGATTGTTTCCTAGGGTTT+TGG 0.194253 2.4:+31789647 MS.gene77173:CDS
ATTCATGAGCTGAGGGGTTT+GGG 0.198657 2.4:+31789702 MS.gene77173:CDS
CATGTTCATCACATCTTTAC+TGG 0.212061 2.4:-31790327 None:intergenic
GATTCATGAGCTGAGGGGTT+TGG 0.264040 2.4:+31789701 MS.gene77173:CDS
CAACACTATATTTCATCCTT+TGG 0.303808 2.4:-31790022 None:intergenic
GAGGTTGATTGGTTATGATT+TGG 0.308965 2.4:+31789968 MS.gene77173:CDS
AGGTCAGGGCGGAGGTTGTT+AGG 0.309694 2.4:+31789739 MS.gene77173:CDS
TTGAAAGGAAAAGGTGCAAT+TGG 0.315697 2.4:+31790125 MS.gene77173:CDS
AAATTCACACCCAAGTATTC+AGG 0.317076 2.4:-31790062 None:intergenic
GATGAAATTGATAGAGTTGT+TGG 0.328831 2.4:+31789627 MS.gene77173:CDS
GAGTTGTTGGATTGTTTCCT+AGG 0.358743 2.4:+31789640 MS.gene77173:CDS
GATGAACATGAAGGCTTTCA+TGG 0.369314 2.4:+31790340 MS.gene77173:CDS
GGTGCAATTGGGTTTGAAGT+TGG 0.399622 2.4:+31790137 MS.gene77173:CDS
ACTCTATCAATTTCATCCTT+AGG 0.408293 2.4:-31789621 None:intergenic
TGGGATTTGGTATTGAGGAT+AGG 0.409253 2.4:+31789667 MS.gene77173:CDS
GAAAGCATCCCGTTGGACTT+TGG 0.413704 2.4:+31790273 MS.gene77173:CDS
TCACATCTTTACTGGTTTGA+GGG 0.416518 2.4:-31790319 None:intergenic
AATTCGTAGGGATGCTTTGA+AGG 0.418729 2.4:+31789929 MS.gene77173:CDS
TGGAAGGAACCGAGGTTGAT+TGG 0.420423 2.4:+31789957 MS.gene77173:CDS
ATCACATCTTTACTGGTTTG+AGG 0.423102 2.4:-31790320 None:intergenic
TGAAAGGAAAAGGTGCAATT+GGG 0.424464 2.4:+31790126 MS.gene77173:CDS
TGTTGCAGAGTTTGAAATGC+AGG 0.431814 2.4:+31789814 MS.gene77173:CDS
GCATGAAGTTCCTGAATACT+TGG 0.444508 2.4:+31790052 MS.gene77173:CDS
CAGCCGAGAGGTCAGGGCGG+AGG 0.450679 2.4:+31789731 MS.gene77173:CDS
ATCTCCATTCCCAAAATCCT+TGG 0.454905 2.4:-31789765 None:intergenic
TACTCAATCACATTGTATCT+AGG 0.456654 2.4:-31790101 None:intergenic
GCGGTCACGGTTTAATTCGT+AGG 0.464329 2.4:+31789916 MS.gene77173:CDS
GTTGATTGGTTATGATTTGG+AGG 0.468999 2.4:+31789971 MS.gene77173:CDS
TTATAAAATCTAAGCCTAGT+TGG 0.470603 2.4:-31790179 None:intergenic
CTCCGCCCTGACCTCTCGGC+TGG 0.473380 2.4:-31789730 None:intergenic
ATTGAGTATTTGAAAGGAAA+AGG 0.475963 2.4:+31790116 MS.gene77173:CDS
GGTCAGGGCGGAGGTTGTTA+GGG 0.481020 2.4:+31789740 MS.gene77173:CDS
AACATCTTCCAAAGTCCAAC+GGG 0.486105 2.4:-31790281 None:intergenic
GTGGATTGTTTGTGCGGTCA+CGG 0.490001 2.4:+31789903 MS.gene77173:CDS
TGTGGAATTTGGGATTCCTA+AGG 0.495755 2.4:+31789605 MS.gene77173:CDS
GGATGAAATATAGTGTTGAT+TGG 0.502115 2.4:+31790027 MS.gene77173:CDS
AGTTGTTGGATTGTTTCCTA+GGG 0.504801 2.4:+31789641 MS.gene77173:CDS
AATGTGATTGAGTATTTGAA+AGG 0.505696 2.4:+31790110 MS.gene77173:CDS
TTTGAGGGAACTTCGGTGGT+TGG 0.508881 2.4:-31790304 None:intergenic
TCATGGACTCGTTGGTGTAG+TGG 0.510176 2.4:+31790357 MS.gene77173:CDS
CGGTCACGGTTTAATTCGTA+GGG 0.517633 2.4:+31789917 MS.gene77173:CDS
TTCATGAGCTGAGGGGTTTG+GGG 0.523718 2.4:+31789703 MS.gene77173:CDS
TTACTGGTTTGAGGGAACTT+CGG 0.536810 2.4:-31790311 None:intergenic
CGGAGGTTGTTAGGGATCCA+AGG 0.538817 2.4:+31789748 MS.gene77173:CDS
GCGATTGATTCATGAGCTGA+GGG 0.554155 2.4:+31789695 MS.gene77173:CDS
CAACCTCCGCCCTGACCTCT+CGG 0.563699 2.4:-31789734 None:intergenic
ATACCAAATCCCAAAACCCT+AGG 0.565652 2.4:-31789657 None:intergenic
GAAGGCTTTCATGGACTCGT+TGG 0.570328 2.4:+31790349 MS.gene77173:CDS
CTGGTTTGAGGGAACTTCGG+TGG 0.572836 2.4:-31790308 None:intergenic
TGCTTTGAAGGTGTTGTGGA+AGG 0.580553 2.4:+31789941 MS.gene77173:CDS
AGCGATTGATTCATGAGCTG+AGG 0.597553 2.4:+31789694 MS.gene77173:CDS
AAGACATTATCAAACCAACT+AGG 0.602970 2.4:+31790165 MS.gene77173:CDS
GTTAAAAGAAAGCATCCCGT+TGG 0.604141 2.4:+31790266 MS.gene77173:CDS
TAAAGATGTGATGAACATGA+AGG 0.609470 2.4:+31790331 MS.gene77173:CDS
GGGATGCTTTGAAGGTGTTG+TGG 0.613256 2.4:+31789937 MS.gene77173:CDS
GTTGCAGAGTTTGAAATGCA+GGG 0.619561 2.4:+31789815 MS.gene77173:CDS
GAACATCTTCCAAAGTCCAA+CGG 0.624427 2.4:-31790282 None:intergenic
CATGAAGTTCCTGAATACTT+GGG 0.630211 2.4:+31790053 MS.gene77173:CDS
CTGAGAGTGGATTGTTTGTG+CGG 0.636549 2.4:+31789897 MS.gene77173:CDS
AAATCATAACCAATCAACCT+CGG 0.663302 2.4:-31789966 None:intergenic
CGATTGATTCATGAGCTGAG+GGG 0.680967 2.4:+31789696 MS.gene77173:CDS
TTCCAGCCGAGAGGTCAGGG+CGG 0.702811 2.4:+31789728 MS.gene77173:CDS
AGGTGTTGTGGAAGGAACCG+AGG 0.744040 2.4:+31789949 MS.gene77173:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTAAAGAGAAGATTTTTG+GGG + chr2.4:31789842-31789861 MS.gene77173:CDS 20.0%
!!! CAATTAAAGAGAAGATTTTT+GGG + chr2.4:31789841-31789860 MS.gene77173:CDS 20.0%
!!! CATATATTTTTTCACATTCA+GGG - chr2.4:31790223-31790242 None:intergenic 20.0%
! AATGTGATTGAGTATTTGAA+AGG + chr2.4:31790110-31790129 MS.gene77173:CDS 25.0%
! ATTGAGTATTTGAAAGGAAA+AGG + chr2.4:31790116-31790135 MS.gene77173:CDS 25.0%
! CCTGAATGTGAAAAAATATA+TGG + chr2.4:31790221-31790240 MS.gene77173:CDS 25.0%
! TTATAAAATCTAAGCCTAGT+TGG - chr2.4:31790182-31790201 None:intergenic 25.0%
!! AGATTGAGTTTTTAATCCAA+AGG + chr2.4:31790006-31790025 MS.gene77173:CDS 25.0%
!! GCAATTAAAGAGAAGATTTT+TGG + chr2.4:31789840-31789859 MS.gene77173:CDS 25.0%
!! TTGATTTTCTTGTGGAATTT+GGG + chr2.4:31789595-31789614 MS.gene77173:CDS 25.0%
!!! ATTGATTTTCTTGTGGAATT+TGG + chr2.4:31789594-31789613 MS.gene77173:CDS 25.0%
!!! CCATATATTTTTTCACATTC+AGG - chr2.4:31790224-31790243 None:intergenic 25.0%
AAATCATAACCAATCAACCT+CGG - chr2.4:31789969-31789988 None:intergenic 30.0%
AAGACATTATCAAACCAACT+AGG + chr2.4:31790165-31790184 MS.gene77173:CDS 30.0%
ACTCTATCAATTTCATCCTT+AGG - chr2.4:31789624-31789643 None:intergenic 30.0%
CAACACTATATTTCATCCTT+TGG - chr2.4:31790025-31790044 None:intergenic 30.0%
GATGAAATTGATAGAGTTGT+TGG + chr2.4:31789627-31789646 MS.gene77173:CDS 30.0%
TAAAGATGTGATGAACATGA+AGG + chr2.4:31790331-31790350 MS.gene77173:CDS 30.0%
TACTCAATCACATTGTATCT+AGG - chr2.4:31790104-31790123 None:intergenic 30.0%
! ATATGGCAGATTTTCAACAA+AGG + chr2.4:31790238-31790257 MS.gene77173:CDS 30.0%
!! GGATGAAATATAGTGTTGAT+TGG + chr2.4:31790027-31790046 MS.gene77173:CDS 30.0%
!!! ATTTTTTCACATTCAGGGTA+AGG - chr2.4:31790218-31790237 None:intergenic 30.0%
!!! TAGTGTCATTGATTTTCTTG+TGG + chr2.4:31789587-31789606 MS.gene77173:CDS 30.0%
AAATTCACACCCAAGTATTC+AGG - chr2.4:31790065-31790084 None:intergenic 35.0%
AGTTGTTGGATTGTTTCCTA+GGG + chr2.4:31789641-31789660 MS.gene77173:CDS 35.0%
CATGAAGTTCCTGAATACTT+GGG + chr2.4:31790053-31790072 MS.gene77173:CDS 35.0%
CATGTTCATCACATCTTTAC+TGG - chr2.4:31790330-31790349 None:intergenic 35.0%
! GAGGTTGATTGGTTATGATT+TGG + chr2.4:31789968-31789987 MS.gene77173:CDS 35.0%
! GTTGATTGGTTATGATTTGG+AGG + chr2.4:31789971-31789990 MS.gene77173:CDS 35.0%
! TGAAAGGAAAAGGTGCAATT+GGG + chr2.4:31790126-31790145 MS.gene77173:CDS 35.0%
! TTGAAAGGAAAAGGTGCAAT+TGG + chr2.4:31790125-31790144 MS.gene77173:CDS 35.0%
!! AAAGAGAAGATTTTTGGGGA+GGG + chr2.4:31789846-31789865 MS.gene77173:CDS 35.0%
!! ATCACATCTTTACTGGTTTG+AGG - chr2.4:31790323-31790342 None:intergenic 35.0%
!! TAAAGAGAAGATTTTTGGGG+AGG + chr2.4:31789845-31789864 MS.gene77173:CDS 35.0%
!! TCACATCTTTACTGGTTTGA+GGG - chr2.4:31790322-31790341 None:intergenic 35.0%
AACATCTTCCAAAGTCCAAC+GGG - chr2.4:31790284-31790303 None:intergenic 40.0%
AATTCGTAGGGATGCTTTGA+AGG + chr2.4:31789929-31789948 MS.gene77173:CDS 40.0%
ATACCAAATCCCAAAACCCT+AGG - chr2.4:31789660-31789679 None:intergenic 40.0%
ATCTCCATTCCCAAAATCCT+TGG - chr2.4:31789768-31789787 None:intergenic 40.0%
GAACATCTTCCAAAGTCCAA+CGG - chr2.4:31790285-31790304 None:intergenic 40.0%
GAGTTGTTGGATTGTTTCCT+AGG + chr2.4:31789640-31789659 MS.gene77173:CDS 40.0%
GATGAACATGAAGGCTTTCA+TGG + chr2.4:31790340-31790359 MS.gene77173:CDS 40.0%
GCATGAAGTTCCTGAATACT+TGG + chr2.4:31790052-31790071 MS.gene77173:CDS 40.0%
! GGATTGTTTCCTAGGGTTTT+GGG + chr2.4:31789648-31789667 MS.gene77173:CDS 40.0%
! GTTAGGGATCCAAGGATTTT+GGG + chr2.4:31789756-31789775 MS.gene77173:CDS 40.0%
! GTTGCAGAGTTTGAAATGCA+GGG + chr2.4:31789815-31789834 MS.gene77173:CDS 40.0%
! TGGAGATTGGGGAATTTTCA+CGG + chr2.4:31789781-31789800 MS.gene77173:CDS 40.0%
! TGGATTGTTTCCTAGGGTTT+TGG + chr2.4:31789647-31789666 MS.gene77173:CDS 40.0%
! TGGGATTTGGTATTGAGGAT+AGG + chr2.4:31789667-31789686 MS.gene77173:CDS 40.0%
! TGTGGAATTTGGGATTCCTA+AGG + chr2.4:31789605-31789624 MS.gene77173:CDS 40.0%
! TGTTAGGGATCCAAGGATTT+TGG + chr2.4:31789755-31789774 MS.gene77173:CDS 40.0%
! TGTTGCAGAGTTTGAAATGC+AGG + chr2.4:31789814-31789833 MS.gene77173:CDS 40.0%
!! AGGATTTTGGGAATGGAGAT+TGG + chr2.4:31789768-31789787 MS.gene77173:CDS 40.0%
!! GATTTTGGGAATGGAGATTG+GGG + chr2.4:31789770-31789789 MS.gene77173:CDS 40.0%
!! GGATTTTGGGAATGGAGATT+GGG + chr2.4:31789769-31789788 MS.gene77173:CDS 40.0%
!! GTTAAAAGAAAGCATCCCGT+TGG + chr2.4:31790266-31790285 MS.gene77173:CDS 40.0%
!! TTACTGGTTTGAGGGAACTT+CGG - chr2.4:31790314-31790333 None:intergenic 40.0%
!!! GGTTTTGGGATTTGGTATTG+AGG + chr2.4:31789662-31789681 MS.gene77173:CDS 40.0%
!!! TTTCCTAGGGTTTTGGGATT+TGG + chr2.4:31789654-31789673 MS.gene77173:CDS 40.0%
!!! TTTTGAAGTGAAGCTGAGAG+TGG + chr2.4:31789884-31789903 MS.gene77173:CDS 40.0%
ATTCATGAGCTGAGGGGTTT+GGG + chr2.4:31789702-31789721 MS.gene77173:CDS 45.0%
CGGTCACGGTTTAATTCGTA+GGG + chr2.4:31789917-31789936 MS.gene77173:CDS 45.0%
! AGCGATTGATTCATGAGCTG+AGG + chr2.4:31789694-31789713 MS.gene77173:CDS 45.0%
! CGATTGATTCATGAGCTGAG+GGG + chr2.4:31789696-31789715 MS.gene77173:CDS 45.0%
! GCGATTGATTCATGAGCTGA+GGG + chr2.4:31789695-31789714 MS.gene77173:CDS 45.0%
! GGTGCAATTGGGTTTGAAGT+TGG + chr2.4:31790137-31790156 MS.gene77173:CDS 45.0%
!! CTGAGAGTGGATTGTTTGTG+CGG + chr2.4:31789897-31789916 MS.gene77173:CDS 45.0%
!! GAAGATTTTTGGGGAGGGTT+TGG + chr2.4:31789851-31789870 MS.gene77173:CDS 45.0%
!! GGATCCAAGGATTTTGGGAA+TGG + chr2.4:31789761-31789780 MS.gene77173:CDS 45.0%
!! TGCTTTGAAGGTGTTGTGGA+AGG + chr2.4:31789941-31789960 MS.gene77173:CDS 45.0%
!! TTTTGGGGAGGGTTTGGTTA+GGG + chr2.4:31789857-31789876 MS.gene77173:CDS 45.0%
!! TTTTTGGGGAGGGTTTGGTT+AGG + chr2.4:31789856-31789875 MS.gene77173:CDS 45.0%
GAAGGCTTTCATGGACTCGT+TGG + chr2.4:31790349-31790368 MS.gene77173:CDS 50.0%
GATTCATGAGCTGAGGGGTT+TGG + chr2.4:31789701-31789720 MS.gene77173:CDS 50.0%
GCGGTCACGGTTTAATTCGT+AGG + chr2.4:31789916-31789935 MS.gene77173:CDS 50.0%
TTCATGAGCTGAGGGGTTTG+GGG + chr2.4:31789703-31789722 MS.gene77173:CDS 50.0%
TTTGAGGGAACTTCGGTGGT+TGG - chr2.4:31790307-31790326 None:intergenic 50.0%
! GTGGATTGTTTGTGCGGTCA+CGG + chr2.4:31789903-31789922 MS.gene77173:CDS 50.0%
! TGGAAGGAACCGAGGTTGAT+TGG + chr2.4:31789957-31789976 MS.gene77173:CDS 50.0%
!! GAAAGCATCCCGTTGGACTT+TGG + chr2.4:31790273-31790292 MS.gene77173:CDS 50.0%
!! GGGATGCTTTGAAGGTGTTG+TGG + chr2.4:31789937-31789956 MS.gene77173:CDS 50.0%
CGGAGGTTGTTAGGGATCCA+AGG + chr2.4:31789748-31789767 MS.gene77173:CDS 55.0%
! AGGTGTTGTGGAAGGAACCG+AGG + chr2.4:31789949-31789968 MS.gene77173:CDS 55.0%
! CTGGTTTGAGGGAACTTCGG+TGG - chr2.4:31790311-31790330 None:intergenic 55.0%
! GTTTTCCAGCCGAGAGGTCA+GGG + chr2.4:31789725-31789744 MS.gene77173:CDS 55.0%
!! TTTGGGGTTTTCCAGCCGAG+AGG + chr2.4:31789719-31789738 MS.gene77173:CDS 55.0%
AGGTCAGGGCGGAGGTTGTT+AGG + chr2.4:31789739-31789758 MS.gene77173:CDS 60.0%
GGTCAGGGCGGAGGTTGTTA+GGG + chr2.4:31789740-31789759 MS.gene77173:CDS 60.0%
! GGTTTTCCAGCCGAGAGGTC+AGG + chr2.4:31789724-31789743 MS.gene77173:CDS 60.0%
CAACCTCCGCCCTGACCTCT+CGG - chr2.4:31789737-31789756 None:intergenic 65.0%
TTCCAGCCGAGAGGTCAGGG+CGG + chr2.4:31789728-31789747 MS.gene77173:CDS 65.0%
CAGCCGAGAGGTCAGGGCGG+AGG + chr2.4:31789731-31789750 MS.gene77173:CDS 75.0%
CTCCGCCCTGACCTCTCGGC+TGG - chr2.4:31789733-31789752 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 31789567 31790376 31789567 ID=MS.gene77173
chr2.4 mRNA 31789567 31790376 31789567 ID=MS.gene77173.t1;Parent=MS.gene77173
chr2.4 exon 31789567 31790376 31789567 ID=MS.gene77173.t1.exon1;Parent=MS.gene77173.t1
chr2.4 CDS 31789567 31790376 31789567 ID=cds.MS.gene77173.t1;Parent=MS.gene77173.t1
Gene Sequence

>MS.gene77173

ATGGGGGAAACTCAAATTGTTAGTGTCATTGATTTTCTTGTGGAATTTGGGATTCCTAAGGATGAAATTGATAGAGTTGTTGGATTGTTTCCTAGGGTTTTGGGATTTGGTATTGAGGATAGGCTGAAGCGATTGATTCATGAGCTGAGGGGTTTGGGGTTTTCCAGCCGAGAGGTCAGGGCGGAGGTTGTTAGGGATCCAAGGATTTTGGGAATGGAGATTGGGGAATTTTCACGGTGTTTGAAGTTGTTGCAGAGTTTGAAATGCAGGGATGCAATTAAAGAGAAGATTTTTGGGGAGGGTTTGGTTAGGGCTTGTTTTGAAGTGAAGCTGAGAGTGGATTGTTTGTGCGGTCACGGTTTAATTCGTAGGGATGCTTTGAAGGTGTTGTGGAAGGAACCGAGGTTGATTGGTTATGATTTGGAGGATATTGAGAAGAAGATTGAGTTTTTAATCCAAAGGATGAAATATAGTGTTGATTGGTTGCATGAAGTTCCTGAATACTTGGGTGTGAATTTTGAAAAACAGATTGTTCCTAGATACAATGTGATTGAGTATTTGAAAGGAAAAGGTGCAATTGGGTTTGAAGTTGGATTGAAAGACATTATCAAACCAACTAGGCTTAGATTTTATAATCTTTATGTCAAGCCTTACCCTGAATGTGAAAAAATATATGGCAGATTTTCAACAAAGGTTGAAGTTAAAAGAAAGCATCCCGTTGGACTTTGGAAGATGTTCCAACCACCGAAGTTCCCTCAAACCAGTAAAGATGTGATGAACATGAAGGCTTTCATGGACTCGTTGGTGTAG

Protein sequence

>MS.gene77173.t1

MGETQIVSVIDFLVEFGIPKDEIDRVVGLFPRVLGFGIEDRLKRLIHELRGLGFSSREVRAEVVRDPRILGMEIGEFSRCLKLLQSLKCRDAIKEKIFGEGLVRACFEVKLRVDCLCGHGLIRRDALKVLWKEPRLIGYDLEDIEKKIEFLIQRMKYSVDWLHEVPEYLGVNFEKQIVPRYNVIEYLKGKGAIGFEVGLKDIIKPTRLRFYNLYVKPYPECEKIYGRFSTKVEVKRKHPVGLWKMFQPPKFPQTSKDVMNMKAFMDSLV