AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene79602


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79602.t1 MTR_7g010210 97.479 238 6 0 30 267 88 325 6.51e-170 472
MS.gene79602.t1 MTR_7g010210 70.293 239 15 3 30 267 88 271 2.31e-109 317
MS.gene79602.t1 MTR_6g012180 57.322 239 73 8 30 256 88 309 9.88e-67 210
MS.gene79602.t1 MTR_3g039990 41.667 240 99 9 30 257 89 299 1.67e-39 139
MS.gene79602.t1 MTR_0140s0030 39.526 253 109 11 30 257 89 322 1.10e-35 130
MS.gene79602.t1 MTR_0489s0020 39.526 253 109 11 30 257 89 322 1.10e-35 130
MS.gene79602.t1 MTR_7g087130 28.179 291 126 10 7 249 68 323 3.95e-15 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79602.t1 AT3G28910 41.909 241 99 8 30 250 12 231 3.83e-39 137
MS.gene79602.t1 AT3G28910 41.909 241 99 8 30 250 88 307 1.10e-37 135
MS.gene79602.t1 AT5G62470 40.467 257 111 6 30 251 87 336 3.14e-36 132
MS.gene79602.t1 AT5G62470 40.467 257 111 6 30 251 88 337 3.37e-36 132
MS.gene79602.t1 AT3G47600 39.689 257 112 9 21 251 77 316 6.23e-35 128
MS.gene79602.t1 AT3G47600 40.476 252 97 9 30 251 82 310 7.97e-35 128
MS.gene79602.t1 AT1G74650 39.526 253 99 10 25 249 1 227 2.45e-28 108
MS.gene79602.t1 AT1G74650 38.057 247 100 9 30 249 12 232 9.72e-28 107
MS.gene79602.t1 AT1G74650 39.676 247 96 9 30 249 88 308 9.16e-27 106
MS.gene79602.t1 AT1G08810 33.745 243 77 8 28 252 3 179 1.26e-21 89.7
MS.gene79602.t1 AT1G08810 33.745 243 77 8 28 252 3 179 1.26e-21 89.7
MS.gene79602.t1 AT1G08810 32.830 265 87 10 7 251 68 261 2.34e-21 90.9
MS.gene79602.t1 AT5G15310 61.538 39 15 0 22 60 1 39 9.75e-12 63.9

Find 41 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATCATGAATACATTATTT+AGG 0.228293 7.3:-87843333 MS.gene79602:intron
AACATAGCTCGTTTACTAAA+AGG 0.252975 7.3:-87842202 MS.gene79602:CDS
CTTCTTTCCCATTGACCTCT+TGG 0.294000 7.3:+87842385 None:intergenic
AAGAACATGGTCCTGGAAAT+TGG 0.298566 7.3:-87843497 None:intergenic
AGTTGTCCTGAAAAGGTATT+AGG 0.303742 7.3:-87841884 MS.gene79602:CDS
GAAACATTTGAATCTTTGTT+TGG 0.358400 7.3:-87842064 MS.gene79602:CDS
ATGTCATTATCTGTTCTTTG+TGG 0.377477 7.3:+87842520 None:intergenic
GTACTAACCGATGATTTGAT+TGG 0.381105 7.3:+87842166 None:intergenic
GAAAATGAGTTACTATTTGT+AGG 0.397920 7.3:+87842274 None:intergenic
GGAACAGCCCTCCAATTTCC+AGG 0.403047 7.3:+87843486 None:intergenic
TATGAACTATTAACCTGTCT+TGG 0.426618 7.3:+87843461 None:intergenic
AGATTGACCTGGTTCTTTAG+TGG 0.427373 7.3:+87842249 None:intergenic
ATGATCAACAACAGTCCTCT+TGG 0.437915 7.3:+87843349 None:intergenic
CCATTTCTCAAGCAAAGAAA+AGG 0.449839 7.3:+87841922 None:intergenic
TCTTGTAAAACCGTTGCGTC+TGG 0.450422 7.3:+87841980 None:intergenic
AACTATTAACCTGTCTTGGT+TGG 0.452548 7.3:+87843465 None:intergenic
AAGAATTCCAAGAGGTCAAT+GGG 0.456644 7.3:-87842392 MS.gene79602:CDS
AAAGAATTCCAAGAGGTCAA+TGG 0.463998 7.3:-87842393 MS.gene79602:CDS
AAAATGAGTTACTATTTGTA+GGG 0.472669 7.3:+87842275 None:intergenic
AGCAGAATCAAAGTTGTTGA+AGG 0.476547 7.3:+87842120 None:intergenic
TGAAGCAAGTTGTCCTGAAA+AGG 0.483879 7.3:-87841891 MS.gene79602:CDS
CATTATCTGTTCTTTGTGGA+AGG 0.487830 7.3:+87842524 None:intergenic
ATTGATTCTGAACCAACATC+AGG 0.495731 7.3:+87841947 None:intergenic
TATATACAAGAACATGGTCC+TGG 0.507906 7.3:-87843504 None:intergenic
TTTGATTCTGCTTCTAGTAG+TGG 0.508963 7.3:-87842109 MS.gene79602:CDS
AGGGCTGTTCCAACCAAGAC+AGG 0.512302 7.3:-87843474 MS.gene79602:intron
AAGCTCAAGAAATCACAAAC+AGG 0.513721 7.3:-87842466 MS.gene79602:CDS
TAGCTCGTTTACTAAAAGGT+TGG 0.524179 7.3:-87842198 MS.gene79602:CDS
TGCTTCTAGTAGTGGAACAA+AGG 0.546510 7.3:-87842101 MS.gene79602:CDS
ACTTCAAACAGATATTCAAA+TGG 0.548282 7.3:-87842362 MS.gene79602:CDS
ATCTTTGTCACCAGACGCAA+CGG 0.551917 7.3:-87841990 MS.gene79602:CDS
CCTGAAAAGGTATTAGGTTG+TGG 0.555878 7.3:-87841878 MS.gene79602:CDS
CATTTCTCAAGCAAAGAAAA+GGG 0.562614 7.3:+87841923 None:intergenic
GCTTCTAGTAGTGGAACAAA+GGG 0.568848 7.3:-87842100 MS.gene79602:CDS
GATGAAAGTAAGCCTGATGT+TGG 0.577667 7.3:-87841959 MS.gene79602:CDS
GATGATTCCAAAGACTCCAA+AGG 0.605819 7.3:+87842034 None:intergenic
AACATGGTCCTGGAAATTGG+AGG 0.614224 7.3:-87843494 MS.gene79602:CDS
ACATGGTCCTGGAAATTGGA+GGG 0.621706 7.3:-87843493 MS.gene79602:CDS
TAGGAAAATCATGATCCAAG+AGG 0.661671 7.3:-87843364 MS.gene79602:CDS
GAAAAGGTATTAGGTTGTGG+TGG 0.702340 7.3:-87841875 MS.gene79602:CDS
GCATCTCAAAGAATTCCAAG+AGG 0.704482 7.3:-87842400 MS.gene79602:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAACTACTAAAAAAAT+TGG + chr7.3:87842722-87842741 None:intergenic 10.0%
!! ATACAAAAAATTATAGAAAT+AGG + chr7.3:87842581-87842600 None:intergenic 10.0%
!! TACAAAAAATTATAGAAATA+GGG + chr7.3:87842580-87842599 None:intergenic 10.0%
!!! ATTAATTTATTTGATTTTTG+TGG - chr7.3:87842115-87842134 MS.gene79602:CDS 10.0%
!!! CAAATTATTTATTTGTTTTA+AGG - chr7.3:87842329-87842348 MS.gene79602:CDS 10.0%
!!! TTTTATTTTTATTACTTAAC+TGG - chr7.3:87842034-87842053 MS.gene79602:CDS 10.0%
!!! TTTTTTAGTAGTTTTTTTTT+TGG - chr7.3:87842724-87842743 MS.gene79602:intron 10.0%
!! ATAATGACATAAAAAACTAT+TGG - chr7.3:87842837-87842856 MS.gene79602:intron 15.0%
!! TTTAAATATCCCTAAAAAAA+AGG - chr7.3:87842622-87842641 MS.gene79602:intron 15.0%
!!! AAATTAGAAGATAATTTTTG+TGG + chr7.3:87843037-87843056 None:intergenic 15.0%
!! AAAATGAGTTACTATTTGTA+GGG + chr7.3:87843072-87843091 None:intergenic 20.0%
!! ATCTTTATGGAATTAAAAAC+AGG + chr7.3:87842499-87842518 None:intergenic 20.0%
!! GTATATCATAGATCATTAAT+AGG - chr7.3:87841961-87841980 MS.gene79602:CDS 20.0%
!! TGATCATGAATACATTATTT+AGG - chr7.3:87842011-87842030 MS.gene79602:CDS 20.0%
!! TTATGAAAGAATATAAGTGT+TGG - chr7.3:87842527-87842546 MS.gene79602:CDS 20.0%
!!! AGCATTTGATTTCATTTTTT+AGG - chr7.3:87842423-87842442 MS.gene79602:CDS 20.0%
!!! ATTAAATTTAGGACATAAGT+TGG - chr7.3:87842463-87842482 MS.gene79602:CDS 20.0%
!!! ATTAGCAAATCCTTTTTTTT+AGG + chr7.3:87842635-87842654 None:intergenic 20.0%
!!! TTAGCAAATCCTTTTTTTTA+GGG + chr7.3:87842634-87842653 None:intergenic 20.0%
!!! TTTTGTGGATTAATTTTTCA+GGG - chr7.3:87842130-87842149 MS.gene79602:CDS 20.0%
!!! TTTTTGTGGATTAATTTTTC+AGG - chr7.3:87842129-87842148 MS.gene79602:CDS 20.0%
! AACATTTATGATGTAACAGA+TGG - chr7.3:87842780-87842799 MS.gene79602:intron 25.0%
! ACATTTATGATGTAACAGAT+GGG - chr7.3:87842781-87842800 MS.gene79602:intron 25.0%
! ACTTCAAACAGATATTCAAA+TGG - chr7.3:87842982-87843001 MS.gene79602:intron 25.0%
! AGGGATATTTAAATCCATTT+AGG + chr7.3:87842615-87842634 None:intergenic 25.0%
! AGTAGCAAATTATGAAGAAA+AGG + chr7.3:87842289-87842308 None:intergenic 25.0%
! CATAACTAGTACTATCTTTA+TGG + chr7.3:87842512-87842531 None:intergenic 25.0%
! GAAAAATCCAATCAAATCAT+CGG - chr7.3:87843171-87843190 MS.gene79602:intron 25.0%
! GAAAATGAGTTACTATTTGT+AGG + chr7.3:87843073-87843092 None:intergenic 25.0%
! GAAACATTTGAATCTTTGTT+TGG - chr7.3:87843280-87843299 MS.gene79602:intron 25.0%
! TAGGGACACAAATTAAAAAT+AGG + chr7.3:87842562-87842581 None:intergenic 25.0%
! TATATTAGACTTGTCCTAAA+TGG - chr7.3:87842598-87842617 MS.gene79602:intron 25.0%
!!! TTCAAGATCTTTTAGGAAAT+AGG - chr7.3:87842259-87842278 MS.gene79602:CDS 25.0%
AACATAGCTCGTTTACTAAA+AGG - chr7.3:87843142-87843161 MS.gene79602:intron 30.0%
ATGTCATTATCTGTTCTTTG+TGG + chr7.3:87842827-87842846 None:intergenic 30.0%
CATTTCTCAAGCAAAGAAAA+GGG + chr7.3:87843424-87843443 None:intergenic 30.0%
TATGAACTATTAACCTGTCT+TGG + chr7.3:87841886-87841905 None:intergenic 30.0%
TTATGATTCACATGTCTAAC+TGG - chr7.3:87842755-87842774 MS.gene79602:intron 30.0%
TTCCTAAAAGATCTTGAAGA+TGG + chr7.3:87842257-87842276 None:intergenic 30.0%
! ATCCATCTTCAAGATCTTTT+AGG - chr7.3:87842252-87842271 MS.gene79602:CDS 30.0%
!! TTAGATGGACTAATTACCTA+AGG - chr7.3:87842181-87842200 MS.gene79602:CDS 30.0%
!!! TGCTTCATTAAGTGCTTTTT+TGG + chr7.3:87843008-87843027 None:intergenic 30.0%
AAAGAATTCCAAGAGGTCAA+TGG - chr7.3:87842951-87842970 MS.gene79602:intron 35.0%
AACTATTAACCTGTCTTGGT+TGG + chr7.3:87841882-87841901 None:intergenic 35.0%
AAGAATTCCAAGAGGTCAAT+GGG - chr7.3:87842952-87842971 MS.gene79602:intron 35.0%
AAGCTCAAGAAATCACAAAC+AGG - chr7.3:87842878-87842897 MS.gene79602:intron 35.0%
AGCAGAATCAAAGTTGTTGA+AGG + chr7.3:87843227-87843246 None:intergenic 35.0%
CATTATCTGTTCTTTGTGGA+AGG + chr7.3:87842823-87842842 None:intergenic 35.0%
CCATTTCTCAAGCAAAGAAA+AGG + chr7.3:87843425-87843444 None:intergenic 35.0%
GTACTAACCGATGATTTGAT+TGG + chr7.3:87843181-87843200 None:intergenic 35.0%
TAGCTCGTTTACTAAAAGGT+TGG - chr7.3:87843146-87843165 MS.gene79602:intron 35.0%
TAGGAAAATCATGATCCAAG+AGG - chr7.3:87841980-87841999 MS.gene79602:CDS 35.0%
TTTGATTCTGCTTCTAGTAG+TGG - chr7.3:87843235-87843254 MS.gene79602:intron 35.0%
! ATTGATTCTGAACCAACATC+AGG + chr7.3:87843400-87843419 None:intergenic 35.0%
! CCTTTTCTTTGCTTGAGAAA+TGG - chr7.3:87843422-87843441 MS.gene79602:intron 35.0%
! TTTTCTTCCACTAAAGAACC+AGG - chr7.3:87843088-87843107 MS.gene79602:intron 35.0%
!! AGTTGTCCTGAAAAGGTATT+AGG - chr7.3:87843460-87843479 MS.gene79602:intron 35.0%
!!! TTTGTTTGGTTTTGAGCCTT+TGG - chr7.3:87843294-87843313 MS.gene79602:intron 35.0%
ATGATCAACAACAGTCCTCT+TGG + chr7.3:87841998-87842017 None:intergenic 40.0%
GATGAAAGTAAGCCTGATGT+TGG - chr7.3:87843385-87843404 MS.gene79602:intron 40.0%
GATGATTCCAAAGACTCCAA+AGG + chr7.3:87843313-87843332 None:intergenic 40.0%
GCAAAAGTTGCAGACTTAGA+TGG - chr7.3:87842166-87842185 MS.gene79602:CDS 40.0%
GCATAGCAAAAAGATTGACC+TGG + chr7.3:87843109-87843128 None:intergenic 40.0%
GCATCTCAAAGAATTCCAAG+AGG - chr7.3:87842944-87842963 MS.gene79602:intron 40.0%
TGAAGCAAGTTGTCCTGAAA+AGG - chr7.3:87843453-87843472 MS.gene79602:intron 40.0%
! AGATTGACCTGGTTCTTTAG+TGG + chr7.3:87843098-87843117 None:intergenic 40.0%
! CCACAACCTAATACCTTTTC+AGG + chr7.3:87843469-87843488 None:intergenic 40.0%
! GCTTCTAGTAGTGGAACAAA+GGG - chr7.3:87843244-87843263 MS.gene79602:intron 40.0%
! TGCTTCTAGTAGTGGAACAA+AGG - chr7.3:87843243-87843262 MS.gene79602:intron 40.0%
!! AAGTTACCCCTTTTGATTCC+TGG + chr7.3:87842207-87842226 None:intergenic 40.0%
!! CCTGAAAAGGTATTAGGTTG+TGG - chr7.3:87843466-87843485 MS.gene79602:intron 40.0%
!! GAAAAGGTATTAGGTTGTGG+TGG - chr7.3:87843469-87843488 MS.gene79602:intron 40.0%
!!! TTTTGAGCCTTTGGAGTCTT+TGG - chr7.3:87843303-87843322 MS.gene79602:intron 40.0%
ACCTAAGGCCAGGAATCAAA+AGG - chr7.3:87842196-87842215 MS.gene79602:CDS 45.0%
ATCTTTGTCACCAGACGCAA+CGG - chr7.3:87843354-87843373 MS.gene79602:CDS 45.0%
CCTAAGGCCAGGAATCAAAA+GGG - chr7.3:87842197-87842216 MS.gene79602:CDS 45.0%
CTAAGGCCAGGAATCAAAAG+GGG - chr7.3:87842198-87842217 MS.gene79602:CDS 45.0%
CTTCTTTCCCATTGACCTCT+TGG + chr7.3:87842962-87842981 None:intergenic 45.0%
GGGTAACTTCACTGAACAAG+AGG - chr7.3:87842218-87842237 MS.gene79602:CDS 45.0%
TCTTGTAAAACCGTTGCGTC+TGG + chr7.3:87843367-87843386 None:intergenic 45.0%
TGGACTAATTACCTAAGGCC+AGG - chr7.3:87842186-87842205 MS.gene79602:CDS 45.0%
!! TTCATTAAAATTATATTTAT+TGG - chr7.3:87841919-87841938 MS.gene79602:CDS 5.0%
!!! AAATTTAATTTTACAAAAAT+TGG + chr7.3:87842452-87842471 None:intergenic 5.0%
!!! ATTTTTGTAAAATTAAATTT+AGG - chr7.3:87842452-87842471 MS.gene79602:CDS 5.0%
!! CCCTTTTGATTCCTGGCCTT+AGG + chr7.3:87842200-87842219 None:intergenic 50.0%
AGGGCTGTTCCAACCAAGAC+AGG - chr7.3:87841870-87841889 MS.gene79602:CDS 55.0%
GGAACAGCCCTCCAATTTCC+AGG + chr7.3:87841861-87841880 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 87841853 87843513 87841853 ID=MS.gene79602
chr7.3 mRNA 87841853 87843513 87841853 ID=MS.gene79602.t1;Parent=MS.gene79602
chr7.3 exon 87843475 87843513 87843475 ID=MS.gene79602.t1.exon1;Parent=MS.gene79602.t1
chr7.3 CDS 87843475 87843513 87843475 ID=cds.MS.gene79602.t1;Parent=MS.gene79602.t1
chr7.3 exon 87843334 87843383 87843334 ID=MS.gene79602.t1.exon2;Parent=MS.gene79602.t1
chr7.3 CDS 87843334 87843383 87843334 ID=cds.MS.gene79602.t1;Parent=MS.gene79602.t1
chr7.3 exon 87841853 87842567 87841853 ID=MS.gene79602.t1.exon3;Parent=MS.gene79602.t1
chr7.3 CDS 87841853 87842567 87841853 ID=cds.MS.gene79602.t1;Parent=MS.gene79602.t1
Gene Sequence

>MS.gene79602

ATGGTCCTGGAAATTGGAGGGCTGTTCCAACCAAGACAGGTTAATAGTTCATAACTTTAAGATTTTTTCATTAAAATTATATTTATTGGCTTTTTTCTTTGTTCTATAGTATATCATAGATCATTAATAGGAAAATCATGATCCAAGAGGACTGTTGTTGATCATGAATACATTATTTAGGTTTTATTTTTATTACTTAACTGGATCTGAATTTAAGTGATTTATTTTTTCTTATTATTATATTTTTATATGCATTGAAGTGATTAATTTATTTGATTTTTGTGGATTAATTTTTCAGGGTTGTCAAGATGCAGCAAAAGTTGCAGACTTAGATGGACTAATTACCTAAGGCCAGGAATCAAAAGGGGTAACTTCACTGAACAAGAGGAGAAGATGATTATCCATCTTCAAGATCTTTTAGGAAATAGGTGAACCTTTTCTTCATAATTTGCTACTTCAAAAAAATAAATTAGCAACAAATTATTTATTTGTTTTAAGGCAGCAACAAAATTTGAATAAGTGATAAGTAAACTATGTTTCTAATTCTGTCATTTTAATTACAAAGAAATTAGCATTTGATTTCATTTTTTAGGTTTCCAATTTTTGTAAAATTAAATTTAGGACATAAGTTGGATATGTGCAACCTGTTTTTAATTCCATAAAGATAGTACTAGTTATGAAAGAATATAAGTGTTGGAATATTTTTCCTATTTTTAATTTGTGTCCCTATTTCTATAATTTTTTGTATATTAGACTTGTCCTAAATGGATTTAAATATCCCTAAAAAAAAGGATTTGCTAATATGTTTGTTTTAGTATTTAATTTAACTTTCTTGATAGAAAACAATATAAGAAAATTAGAGACTTCCAATTTTTTTAGTAGTTTTTTTTTTGGTAGCTTCTTTATGATTCACATGTCTAACTGGATAACATTTATGATGTAACAGATGGGCTGCAATAGCATCATACCTTCCACAAAGAACAGATAATGACATAAAAAACTATTGGAATACTCATTTGAAAAAGAAGCTCAAGAAATCACAAACAGGTAATAATGCTGAATGCATTAATTTTGAAGAAGAAAAATTTTCAGCATCTCAAAGAATTCCAAGAGGTCAATGGGAAAGAAGACTTCAAACAGATATTCAAATGGCCAAAAAAGCACTTAATGAAGCACTTTCACCACAAAAATTATCTTCTAATTTTTCTACTTCAAACCCTACAAATAGTAACTCATTTTCTTCCACTAAAGAACCAGGTCAATCTTTTTGCTATGCTTCAAGTGCAGATAACATAGCTCGTTTACTAAAAGGTTGGATGAAAAATCCAATCAAATCATCGGTTAGTACTAATTCTGCTGTGACACAAAATTCCTTCAACAACTTTGATTCTGCTTCTAGTAGTGGAACAAAGGGTGAAATATTGTCTGAAACATTTGAATCTTTGTTTGGTTTTGAGCCTTTGGAGTCTTTGGAATCATCAAATTCTGAATTCTCTCGTAAATCTTTGTCACCAGACGCAACGGTTTTACAAGATGAAAGTAAGCCTGATGTTGGTTCAGAATCAATGCCCTTTTCTTTGCTTGAGAAATGGCTTCTTGATGAAGCAAGTTGTCCTGAAAAGGTATTAGGTTGTGGTGGTGATCATGCTCAAGTTTTCTAG

Protein sequence

>MS.gene79602.t1

MVLEIGGLFQPRQENHDPRGLLLIMNTLFRWAAIASYLPQRTDNDIKNYWNTHLKKKLKKSQTGNNAECINFEEEKFSASQRIPRGQWERRLQTDIQMAKKALNEALSPQKLSSNFSTSNPTNSNSFSSTKEPGQSFCYASSADNIARLLKGWMKNPIKSSVSTNSAVTQNSFNNFDSASSSGTKGEILSETFESLFGFEPLESLESSNSEFSRKSLSPDATVLQDESKPDVGSESMPFSLLEKWLLDEASCPEKVLGCGGDHAQVF