Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80908.t1 | XP_013447505.1 | 96.4 | 304 | 11 | 0 | 91 | 394 | 94 | 397 | 2.20E-156 | 562 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80908.t1 | Q9QYW0 | 29.5 | 312 | 179 | 9 | 93 | 365 | 183 | 492 | 3.4e-25 | 117.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80908.t1 | A0A072TWJ8 | 96.4 | 304 | 11 | 0 | 91 | 394 | 94 | 397 | 1.6e-156 | 562.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049825 | MS.gene80908 | 0.803152 | 3.99E-49 | -1.69E-46 |
| MS.gene050303 | MS.gene80908 | 0.815951 | 7.10E-52 | -1.69E-46 |
| MS.gene052947 | MS.gene80908 | 0.801164 | 1.02E-48 | -1.69E-46 |
| MS.gene053080 | MS.gene80908 | 0.804668 | 1.93E-49 | -1.69E-46 |
| MS.gene054657 | MS.gene80908 | 0.802463 | 5.54E-49 | -1.69E-46 |
| MS.gene05781 | MS.gene80908 | 0.800995 | 1.11E-48 | -1.69E-46 |
| MS.gene05954 | MS.gene80908 | 0.810559 | 1.09E-50 | -1.69E-46 |
| MS.gene059830 | MS.gene80908 | 0.800953 | 1.13E-48 | -1.69E-46 |
| MS.gene060763 | MS.gene80908 | 0.805786 | 1.13E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene80908 | MS.gene053381 | PPI |
| MS.gene80908 | MS.gene029825 | PPI |
| MS.gene80908 | MS.gene70303 | PPI |
| MS.gene80908 | MS.gene003175 | PPI |
| MS.gene80908 | MS.gene28161 | PPI |
| MS.gene80908 | MS.gene065496 | PPI |
| MS.gene80908 | MS.gene65479 | PPI |
| MS.gene80908 | MS.gene88378 | PPI |
| MS.gene80908 | MS.gene28303 | PPI |
| MS.gene80908 | MS.gene062301 | PPI |
| MS.gene80908 | MS.gene003846 | PPI |
| MS.gene80908 | MS.gene28304 | PPI |
| MS.gene031356 | MS.gene80908 | PPI |
| MS.gene80908 | MS.gene75013 | PPI |
| MS.gene80908 | MS.gene063209 | PPI |
| MS.gene80908 | MS.gene063568 | PPI |
| MS.gene017174 | MS.gene80908 | PPI |
| MS.gene80908 | MS.gene038409 | PPI |
| MS.gene80908 | MS.gene45448 | PPI |
| MS.gene80908 | MS.gene02854 | PPI |
| MS.gene80908 | MS.gene58159 | PPI |
| MS.gene80908 | MS.gene030707 | PPI |
| MS.gene80908 | MS.gene056617 | PPI |
| MS.gene80908 | MS.gene048301 | PPI |
| MS.gene80908 | MS.gene01984 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80908.t1 | MTR_5g064850 | 95.833 | 336 | 14 | 0 | 59 | 394 | 62 | 397 | 0.0 | 676 |
| MS.gene80908.t1 | MTR_7g007890 | 96.142 | 337 | 13 | 0 | 58 | 394 | 61 | 397 | 0.0 | 675 |
| MS.gene80908.t1 | MTR_5g064850 | 95.536 | 336 | 13 | 1 | 59 | 394 | 62 | 395 | 0.0 | 667 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80908.t1 | AT5G61330 | 61.096 | 365 | 130 | 5 | 30 | 393 | 83 | 436 | 1.12e-142 | 413 |
| MS.gene80908.t1 | AT5G61330 | 61.096 | 365 | 130 | 5 | 30 | 393 | 83 | 436 | 1.12e-142 | 413 |
| MS.gene80908.t1 | AT5G61330 | 61.096 | 365 | 130 | 5 | 30 | 393 | 94 | 447 | 1.46e-142 | 413 |
Find 73 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGCAGTATGGCAACTTTC+AGG | 0.156523 | 7.3:-89638619 | MS.gene80908:intron |
| AGATCAGCTATTAAAATATT+TGG | 0.170387 | 7.3:-89638360 | MS.gene80908:CDS |
| TTTAGAAGTTTCTAACAATT+TGG | 0.183126 | 7.3:-89639060 | MS.gene80908:CDS |
| TGAAAAGATAGTAAACTTTA+TGG | 0.190174 | 7.3:-89635857 | MS.gene80908:CDS |
| ACTGGTGCTGCTGCAATTAA+AGG | 0.238779 | 7.3:-89638554 | MS.gene80908:CDS |
| GGAGACTTTGGATTCTATAT+TGG | 0.273642 | 7.3:-89640699 | MS.gene80908:CDS |
| CATGAAGAACTCACCATTAC+TGG | 0.297401 | 7.3:+89640073 | None:intergenic |
| ATGAATTGCTACCTCGTTAT+AGG | 0.297845 | 7.3:+89643402 | None:intergenic |
| CATCTGCTTGATCATTCTAC+TGG | 0.336534 | 7.3:+89638392 | None:intergenic |
| AACTTCTTCCAAGGAGACTT+TGG | 0.353164 | 7.3:-89640711 | MS.gene80908:CDS |
| TCATATAAGCAGCAACTTGA+TGG | 0.354843 | 7.3:+89638421 | None:intergenic |
| AATCTTACAGCTCCTGTTCC+TGG | 0.355537 | 7.3:+89643142 | None:intergenic |
| TCAGAATCGTCTAGAAGTTC+AGG | 0.365325 | 7.3:+89637444 | None:intergenic |
| ATGCTATTTGATGATCTGTT+TGG | 0.374783 | 7.3:-89635799 | MS.gene80908:CDS |
| TGATAGCGAGTTGGAGGAGG+AGG | 0.397733 | 7.3:-89643295 | MS.gene80908:CDS |
| AGCCATTCTCAAATTCAAAT+AGG | 0.416090 | 7.3:-89640895 | MS.gene80908:CDS |
| ACAACTTGAGAAAGAGTATA+GGG | 0.419407 | 7.3:-89643175 | MS.gene80908:CDS |
| TGAGGGAAGTGACGAGGAAG+AGG | 0.420724 | 7.3:-89643244 | MS.gene80908:CDS |
| GAAGAACTTGAAGCATCATA+AGG | 0.425208 | 7.3:-89641354 | MS.gene80908:CDS |
| GAATTAGATGAGACAGTTAG+AGG | 0.428056 | 7.3:-89640752 | MS.gene80908:CDS |
| TTGCAGTATGGCAACTTTCA+GGG | 0.436730 | 7.3:-89638618 | MS.gene80908:intron |
| GCCATGGGAGGAAGATTTGC+CGG | 0.449865 | 7.3:+89635826 | None:intergenic |
| AGCCTATTTGAATTTGAGAA+TGG | 0.451198 | 7.3:+89640893 | None:intergenic |
| TGGATGGAAAGATGATGAAA+TGG | 0.453888 | 7.3:-89643199 | MS.gene80908:CDS |
| ACAATTGATCCTTCATCATC+AGG | 0.454754 | 7.3:-89637387 | MS.gene80908:intron |
| AGCAGAAGAAGATGATGGAC+AGG | 0.458004 | 7.3:-89643268 | MS.gene80908:CDS |
| AGATTTCTCGGATGCACAAC+AGG | 0.459754 | 7.3:-89639010 | MS.gene80908:intron |
| GATGAGGGGCAAGAGGAAAG+TGG | 0.465440 | 7.3:-89643219 | MS.gene80908:CDS |
| AAGAGTGGTCACAGATTTCT+CGG | 0.468215 | 7.3:-89639022 | MS.gene80908:CDS |
| CACAGTCAACCGTGATTATG+AGG | 0.470493 | 7.3:+89635747 | None:intergenic |
| ATCAAATAGCATTGGCGCCA+TGG | 0.471359 | 7.3:+89635810 | None:intergenic |
| TGATGATAGCGAGTTGGAGG+AGG | 0.473123 | 7.3:-89643298 | MS.gene80908:CDS |
| GGATCTTCATCACCAGGAAC+AGG | 0.478572 | 7.3:-89643154 | MS.gene80908:CDS |
| TATAGAATCCAAAGTCTCCT+TGG | 0.480413 | 7.3:+89640703 | None:intergenic |
| GAAGATGATGGACAGGATGA+GGG | 0.488543 | 7.3:-89643261 | MS.gene80908:CDS |
| AGAAGATGATGGACAGGATG+AGG | 0.489022 | 7.3:-89643262 | MS.gene80908:CDS |
| TAATGATAGCTCTAATGAAC+CGG | 0.494552 | 7.3:-89638232 | MS.gene80908:intron |
| AGAGGAAGATGAGGGGCAAG+AGG | 0.494847 | 7.3:-89643226 | MS.gene80908:CDS |
| AAACTTTATGGCTCCTCGAC+CGG | 0.496516 | 7.3:-89635845 | MS.gene80908:CDS |
| AGGATGACACAAGTGACAAC+TGG | 0.501309 | 7.3:-89638572 | MS.gene80908:CDS |
| AGGATGAAGATCTCCTGAAA+GGG | 0.503386 | 7.3:-89641334 | MS.gene80908:CDS |
| AATTGTTTACCTGATGATGA+AGG | 0.510261 | 7.3:+89637378 | None:intergenic |
| GATAGCGAGTTGGAGGAGGA+GGG | 0.511948 | 7.3:-89643294 | MS.gene80908:CDS |
| TAATGATGATAGCGAGTTGG+AGG | 0.512371 | 7.3:-89643301 | MS.gene80908:CDS |
| AACAACTTGAGAAAGAGTAT+AGG | 0.516904 | 7.3:-89643176 | MS.gene80908:CDS |
| TGACGAGGAAGAGGAAGATG+AGG | 0.520219 | 7.3:-89643235 | MS.gene80908:CDS |
| ACCGGCAAATCTTCCTCCCA+TGG | 0.522237 | 7.3:-89635827 | MS.gene80908:CDS |
| GGGACAAGTCAATCAACAAA+TGG | 0.528981 | 7.3:-89638598 | MS.gene80908:CDS |
| TCAAATAGCATTGGCGCCAT+GGG | 0.534558 | 7.3:+89635811 | None:intergenic |
| GGAGGAAGATTTGCCGGTCG+AGG | 0.535642 | 7.3:+89635832 | None:intergenic |
| TTCTATATTGGAACTTCAAG+AGG | 0.536268 | 7.3:-89640687 | MS.gene80908:intron |
| AACAGATCATCAAATAGCAT+TGG | 0.546718 | 7.3:+89635802 | None:intergenic |
| GGTTAATGATGATAGCGAGT+TGG | 0.548944 | 7.3:-89643304 | MS.gene80908:CDS |
| ATCTGCTTGATCATTCTACT+GGG | 0.552705 | 7.3:+89638393 | None:intergenic |
| AGGGGCAAGAGGAAAGTGGA+TGG | 0.557551 | 7.3:-89643215 | MS.gene80908:CDS |
| GGAGAAGCAGAAGAAGATGA+TGG | 0.561538 | 7.3:-89643273 | MS.gene80908:CDS |
| AGATTTGATAACTTCTTCCA+AGG | 0.564786 | 7.3:-89640720 | MS.gene80908:CDS |
| TTACTGGATTGAGCAACTGA+TGG | 0.566488 | 7.3:+89640089 | None:intergenic |
| AAGGATGAAGATCTCCTGAA+AGG | 0.581931 | 7.3:-89641335 | MS.gene80908:CDS |
| GACGAGGAAGAGGAAGATGA+GGG | 0.588247 | 7.3:-89643234 | MS.gene80908:CDS |
| AATAGCATTGGCGCCATGGG+AGG | 0.603352 | 7.3:+89635814 | None:intergenic |
| TCAGTTGCTCAATCCAGTAA+TGG | 0.604366 | 7.3:-89640086 | MS.gene80908:intron |
| ATTAGAGCTATCATTAACCT+CGG | 0.615774 | 7.3:+89638239 | None:intergenic |
| AAATTCAAATAGGCTACCCC+AGG | 0.616563 | 7.3:-89640885 | MS.gene80908:intron |
| GTATAGGGATCTTCATCACC+AGG | 0.617577 | 7.3:-89643160 | MS.gene80908:CDS |
| AGTCAATCAACAAATGGCAG+AGG | 0.633983 | 7.3:-89638592 | MS.gene80908:CDS |
| ATGACAATCTTGATCAAGAG+TGG | 0.634506 | 7.3:-89639037 | MS.gene80908:CDS |
| CTTATGTATCCAGGTTGCCG+AGG | 0.638008 | 7.3:-89638256 | MS.gene80908:intron |
| ACGAGGAAGAGGAAGATGAG+GGG | 0.663371 | 7.3:-89643233 | MS.gene80908:CDS |
| ACAGGATGAGGGAAGTGACG+AGG | 0.677129 | 7.3:-89643250 | MS.gene80908:CDS |
| GCAAGCTGTGAAAAGCCAGA+AGG | 0.680270 | 7.3:-89641312 | MS.gene80908:intron |
| TGATGATATGTCCTATAACG+AGG | 0.728032 | 7.3:-89643413 | MS.gene80908:intron |
| GCTCAGGAAACACATGCTGA+CGG | 0.737841 | 7.3:-89637471 | MS.gene80908:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 89635762 | 89643479 | 89635762 | ID=MS.gene80908 |
| chr7.3 | mRNA | 89635762 | 89643479 | 89635762 | ID=MS.gene80908.t1;Parent=MS.gene80908 |
| chr7.3 | exon | 89643414 | 89643479 | 89643414 | ID=MS.gene80908.t1.exon1;Parent=MS.gene80908.t1 |
| chr7.3 | CDS | 89643414 | 89643479 | 89643414 | ID=cds.MS.gene80908.t1;Parent=MS.gene80908.t1 |
| chr7.3 | exon | 89643150 | 89643325 | 89643150 | ID=MS.gene80908.t1.exon2;Parent=MS.gene80908.t1 |
| chr7.3 | CDS | 89643150 | 89643325 | 89643150 | ID=cds.MS.gene80908.t1;Parent=MS.gene80908.t1 |
| chr7.3 | exon | 89641313 | 89641385 | 89641313 | ID=MS.gene80908.t1.exon3;Parent=MS.gene80908.t1 |
| chr7.3 | CDS | 89641313 | 89641385 | 89641313 | ID=cds.MS.gene80908.t1;Parent=MS.gene80908.t1 |
| chr7.3 | exon | 89640886 | 89640960 | 89640886 | ID=MS.gene80908.t1.exon4;Parent=MS.gene80908.t1 |
| chr7.3 | CDS | 89640886 | 89640960 | 89640886 | ID=cds.MS.gene80908.t1;Parent=MS.gene80908.t1 |
| chr7.3 | exon | 89640688 | 89640798 | 89640688 | ID=MS.gene80908.t1.exon5;Parent=MS.gene80908.t1 |
| chr7.3 | CDS | 89640688 | 89640798 | 89640688 | ID=cds.MS.gene80908.t1;Parent=MS.gene80908.t1 |
| chr7.3 | exon | 89640087 | 89640129 | 89640087 | ID=MS.gene80908.t1.exon6;Parent=MS.gene80908.t1 |
| chr7.3 | CDS | 89640087 | 89640129 | 89640087 | ID=cds.MS.gene80908.t1;Parent=MS.gene80908.t1 |
| chr7.3 | exon | 89639011 | 89639104 | 89639011 | ID=MS.gene80908.t1.exon7;Parent=MS.gene80908.t1 |
| chr7.3 | CDS | 89639011 | 89639104 | 89639011 | ID=cds.MS.gene80908.t1;Parent=MS.gene80908.t1 |
| chr7.3 | exon | 89638532 | 89638634 | 89638532 | ID=MS.gene80908.t1.exon8;Parent=MS.gene80908.t1 |
| chr7.3 | CDS | 89638532 | 89638634 | 89638532 | ID=cds.MS.gene80908.t1;Parent=MS.gene80908.t1 |
| chr7.3 | exon | 89638356 | 89638451 | 89638356 | ID=MS.gene80908.t1.exon9;Parent=MS.gene80908.t1 |
| chr7.3 | CDS | 89638356 | 89638451 | 89638356 | ID=cds.MS.gene80908.t1;Parent=MS.gene80908.t1 |
| chr7.3 | exon | 89638233 | 89638265 | 89638233 | ID=MS.gene80908.t1.exon10;Parent=MS.gene80908.t1 |
| chr7.3 | CDS | 89638233 | 89638265 | 89638233 | ID=cds.MS.gene80908.t1;Parent=MS.gene80908.t1 |
| chr7.3 | exon | 89637388 | 89637487 | 89637388 | ID=MS.gene80908.t1.exon11;Parent=MS.gene80908.t1 |
| chr7.3 | CDS | 89637388 | 89637487 | 89637388 | ID=cds.MS.gene80908.t1;Parent=MS.gene80908.t1 |
| chr7.3 | exon | 89635989 | 89636073 | 89635989 | ID=MS.gene80908.t1.exon12;Parent=MS.gene80908.t1 |
| chr7.3 | CDS | 89635989 | 89636073 | 89635989 | ID=cds.MS.gene80908.t1;Parent=MS.gene80908.t1 |
| chr7.3 | exon | 89635762 | 89635891 | 89635762 | ID=MS.gene80908.t1.exon13;Parent=MS.gene80908.t1 |
| chr7.3 | CDS | 89635762 | 89635891 | 89635762 | ID=cds.MS.gene80908.t1;Parent=MS.gene80908.t1 |
| Gene Sequence |
| Protein sequence |
