AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82542


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82542.t1 MTR_3g070970 99.618 262 1 0 1 262 1 262 0.0 536
MS.gene82542.t1 MTR_1g093750 45.503 189 94 3 66 254 151 330 2.55e-43 150
MS.gene82542.t1 MTR_2g015890 44.444 144 66 3 73 215 67 197 2.91e-28 108
MS.gene82542.t1 MTR_4g079760 35.533 197 106 4 65 254 60 242 4.86e-24 97.4
MS.gene82542.t1 MTR_2g015890 46.667 120 51 2 73 192 67 173 4.87e-24 96.7
MS.gene82542.t1 MTR_4g087920 33.668 199 112 6 67 255 69 257 5.11e-23 95.1
MS.gene82542.t1 MTR_2g091190 34.054 185 105 4 63 240 49 223 5.56e-23 94.7
MS.gene82542.t1 MTR_5g032375 35.028 177 85 5 73 236 45 204 1.12e-19 85.1
MS.gene82542.t1 MTR_5g066080 29.596 223 107 5 51 234 7 218 2.35e-16 76.6
MS.gene82542.t1 MTR_2g015890 39.474 114 51 3 105 215 1 99 2.57e-15 72.0
MS.gene82542.t1 MTR_2g015890 41.111 90 36 2 105 192 1 75 2.46e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82542.t1 AT2G40200 44.245 278 108 10 3 261 1 250 2.39e-54 176
MS.gene82542.t1 AT1G68810 41.538 195 98 4 66 255 167 350 3.76e-36 132
MS.gene82542.t1 AT3G56770 36.757 185 105 4 61 243 33 207 5.52e-34 123
MS.gene82542.t1 AT3G25710 46.405 153 70 2 69 220 128 269 6.59e-34 125
MS.gene82542.t1 AT2G41130 34.973 183 106 4 63 242 57 229 2.75e-25 100
MS.gene82542.t1 AT4G38070 33.108 148 82 3 73 220 92 222 1.50e-18 82.8

Find 36 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGATGATGAAGCAATTT+TGG 0.183435 3.1:-58234294 None:intergenic
TATATTGGTGCTTTGTTCTA+AGG 0.203318 3.1:+58234214 MS.gene82542:CDS
ATTTCCCATTTGGATGAGTA+TGG 0.281846 3.1:+58232695 None:intergenic
GTCTTAAACCTTTGAGGACT+TGG 0.282951 3.1:-58234142 None:intergenic
AATATCTCTGACTCTTCTTC+TGG 0.300411 3.1:+58232828 MS.gene82542:CDS
ATTAGTTCAGAGAACAATTC+TGG 0.307068 3.1:-58234119 None:intergenic
TTTGGTCACTGCTGATTTAA+GGG 0.341399 3.1:-58234276 None:intergenic
TTGTAAGTCTTAAACCTTTG+AGG 0.389696 3.1:-58234148 None:intergenic
CTCTGCTTGACTATGACTCT+TGG 0.398647 3.1:-58232928 None:intergenic
CTCTGCCTCTTACTTCTTGT+AGG 0.400975 3.1:-58234329 None:intergenic
ATGGACAAAGCAGCATTACT+AGG 0.419485 3.1:+58233903 MS.gene82542:CDS
TCTTCAACACTCCCTTCAAT+AGG 0.436660 3.1:-58232888 None:intergenic
TGGCAGAATCAAAAGTATAT+TGG 0.438346 3.1:+58234199 MS.gene82542:CDS
TTTGTTCACTTTGTTAGTGT+TGG 0.440377 3.1:-58234048 None:intergenic
GAAGAGTCAGAGATATTATG+AGG 0.460214 3.1:-58232822 None:intergenic
TGATTTACCTTATCAGACTT+AGG 0.462054 3.1:-58233005 None:intergenic
CAAAATTGCTTCATCATCAA+TGG 0.471187 3.1:+58234295 MS.gene82542:CDS
GGCAGCATTTGAGCACAAAC+TGG 0.479615 3.1:+58232742 MS.gene82542:CDS
ACTTATTCCTAAGTCTGATA+AGG 0.480745 3.1:+58232998 MS.gene82542:CDS
GATACTTCATCAATTTCAGT+TGG 0.481484 3.1:-58233993 None:intergenic
TCATGGAATTTACCTATTGA+AGG 0.482372 3.1:+58232876 MS.gene82542:CDS
CAGAGAAAAGACGTAGAGAC+AGG 0.494900 3.1:+58232946 MS.gene82542:CDS
GCCGACATAGCAAGTGTTGG+TGG 0.503196 3.1:+58234179 MS.gene82542:CDS
GCCACCAACACTTGCTATGT+CGG 0.503342 3.1:-58234180 None:intergenic
AATATAGTGAATTTCAGTCA+TGG 0.537343 3.1:+58232859 MS.gene82542:CDS
CATGGAATTTACCTATTGAA+GGG 0.539204 3.1:+58232877 MS.gene82542:CDS
ATCTGTGTAGGTACAGTCAC+AGG 0.552887 3.1:-58232795 None:intergenic
AGATCTAAAGCGAAAAGCAA+TGG 0.560117 3.1:+58233947 MS.gene82542:CDS
ATTGTCCTACAAGAAGTAAG+AGG 0.570567 3.1:+58234324 MS.gene82542:CDS
GAACTAATCCAAGTCCTCAA+AGG 0.579974 3.1:+58234134 MS.gene82542:CDS
TGGACAAAGCAGCATTACTA+GGG 0.580902 3.1:+58233904 MS.gene82542:CDS
TGGTTTCATCTTCAACAACA+TGG 0.610583 3.1:-58234028 None:intergenic
AATTACTATACAAAATGAGA+AGG 0.613826 3.1:-58234362 None:intergenic
TTATGAGGTAGTATCTGTGT+AGG 0.633198 3.1:-58232807 None:intergenic
AAAGCCGACATAGCAAGTGT+TGG 0.637609 3.1:+58234176 MS.gene82542:CDS
ACTACAATGATTCTTCTGAG+TGG 0.704840 3.1:+58232721 MS.gene82542:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTTTTTTTTTTAAAAA+TGG + chr3.1:58233556-58233575 MS.gene82542:intron 0.0%
!! CTGAAAATAATTTGAGAAAA+TGG - chr3.1:58233714-58233733 None:intergenic 20.0%
!!! AAAATTTTCTAGATTCATTG+TGG + chr3.1:58233424-58233443 MS.gene82542:intron 20.0%
!!! TACAATTTTCTGCAATATTA+AGG + chr3.1:58233811-58233830 MS.gene82542:intron 20.0%
! AAAATGGAATTACCTACTTA+GGG + chr3.1:58233130-58233149 MS.gene82542:intron 25.0%
! AATATAGTGAATTTCAGTCA+TGG + chr3.1:58232859-58232878 MS.gene82542:CDS 25.0%
! ACAAATGATTATGTTATGCA+AGG - chr3.1:58233873-58233892 None:intergenic 25.0%
! ACATATTTCATGCAAGAAAA+TGG + chr3.1:58233114-58233133 MS.gene82542:intron 25.0%
! CAAATGATTATGTTATGCAA+GGG - chr3.1:58233872-58233891 None:intergenic 25.0%
! TATAATTCAAAGCATGTACT+TGG + chr3.1:58233288-58233307 MS.gene82542:intron 25.0%
!! ATCCTATATTTTCTCCAATA+GGG - chr3.1:58233512-58233531 None:intergenic 25.0%
!! TCAAATATCTTATTTCGTAC+CGG - chr3.1:58233594-58233613 None:intergenic 25.0%
!! TCTATTTCTTATTGTGTGTT+TGG + chr3.1:58233352-58233371 MS.gene82542:intron 25.0%
AATGCAACATCTTATCACTT+GGG - chr3.1:58233160-58233179 None:intergenic 30.0%
ACTTATTCCTAAGTCTGATA+AGG + chr3.1:58232998-58233017 MS.gene82542:CDS 30.0%
AGTAAAATTGCCAGAATAAC+GGG - chr3.1:58233453-58233472 None:intergenic 30.0%
AGTCTTGAGTATGAACAATT+TGG + chr3.1:58233327-58233346 MS.gene82542:intron 30.0%
ATCATTTGTATACTTGCAGA+TGG + chr3.1:58233884-58233903 MS.gene82542:intron 30.0%
ATGTTGCATTCATTCAATTC+AGG + chr3.1:58233170-58233189 MS.gene82542:intron 30.0%
ATTAGTTCAGAGAACAATTC+TGG - chr3.1:58234122-58234141 None:intergenic 30.0%
CAAAATTGCTTCATCATCAA+TGG + chr3.1:58234295-58234314 MS.gene82542:CDS 30.0%
CATGGAATTTACCTATTGAA+GGG + chr3.1:58232877-58232896 MS.gene82542:CDS 30.0%
GAAAATGGAATTACCTACTT+AGG + chr3.1:58233129-58233148 MS.gene82542:intron 30.0%
GAGTAAAATTGCCAGAATAA+CGG - chr3.1:58233454-58233473 None:intergenic 30.0%
GATACTTCATCAATTTCAGT+TGG - chr3.1:58233996-58234015 None:intergenic 30.0%
GCACTAATACTTCGTATTAA+AGG + chr3.1:58233630-58233649 MS.gene82542:intron 30.0%
GGACTTACATAAAATGTTGT+TGG + chr3.1:58233242-58233261 MS.gene82542:intron 30.0%
TCATGGAATTTACCTATTGA+AGG + chr3.1:58232876-58232895 MS.gene82542:CDS 30.0%
TGATTTACCTTATCAGACTT+AGG - chr3.1:58233008-58233027 None:intergenic 30.0%
TTATTGTGTGTTTGGATTGA+TGG + chr3.1:58233360-58233379 MS.gene82542:intron 30.0%
TTGTAAGTCTTAAACCTTTG+AGG - chr3.1:58234151-58234170 None:intergenic 30.0%
TTTGTTCACTTTGTTAGTGT+TGG - chr3.1:58234051-58234070 None:intergenic 30.0%
! ATTTTCAGTGTGTACATGTT+TGG + chr3.1:58233726-58233745 MS.gene82542:intron 30.0%
! CATCCTATATTTTCTCCAAT+AGG - chr3.1:58233513-58233532 None:intergenic 30.0%
! CATTTTATGTAAGTCCCAAA+AGG - chr3.1:58233238-58233257 None:intergenic 30.0%
! TATATTGGTGCTTTGTTCTA+AGG + chr3.1:58234214-58234233 MS.gene82542:CDS 30.0%
! TCCTATATTTTCTCCAATAG+GGG - chr3.1:58233511-58233530 None:intergenic 30.0%
! TGGCAGAATCAAAAGTATAT+TGG + chr3.1:58234199-58234218 MS.gene82542:CDS 30.0%
! TTTGACAAAAGCTACCTTTT+GGG + chr3.1:58233221-58233240 MS.gene82542:intron 30.0%
! TTTTGACAAAAGCTACCTTT+TGG + chr3.1:58233220-58233239 MS.gene82542:intron 30.0%
!!! CATTGATGATGAAGCAATTT+TGG - chr3.1:58234297-58234316 None:intergenic 30.0%
AATATCTCTGACTCTTCTTC+TGG + chr3.1:58232828-58232847 MS.gene82542:CDS 35.0%
ACTACAATGATTCTTCTGAG+TGG + chr3.1:58232721-58232740 MS.gene82542:CDS 35.0%
ATCAATTCCTGATGCATGAA+TGG - chr3.1:58233480-58233499 None:intergenic 35.0%
ATTGTCCTACAAGAAGTAAG+AGG + chr3.1:58234324-58234343 MS.gene82542:CDS 35.0%
GAAGAGTCAGAGATATTATG+AGG - chr3.1:58232825-58232844 None:intergenic 35.0%
GAATGCAACATCTTATCACT+TGG - chr3.1:58233161-58233180 None:intergenic 35.0%
GTAAAATTGCCAGAATAACG+GGG - chr3.1:58233452-58233471 None:intergenic 35.0%
TCCCCTATTGGAGAAAATAT+AGG + chr3.1:58233507-58233526 MS.gene82542:intron 35.0%
TCGTATTAGTACCTGAAAGT+GGG - chr3.1:58233660-58233679 None:intergenic 35.0%
TGGTTTCATCTTCAACAACA+TGG - chr3.1:58234031-58234050 None:intergenic 35.0%
TTATGAGGTAGTATCTGTGT+AGG - chr3.1:58232810-58232829 None:intergenic 35.0%
! AGATCTAAAGCGAAAAGCAA+TGG + chr3.1:58233947-58233966 MS.gene82542:CDS 35.0%
!! TTTGGTCACTGCTGATTTAA+GGG - chr3.1:58234279-58234298 None:intergenic 35.0%
!!! TTTTGGTCACTGCTGATTTA+AGG - chr3.1:58234280-58234299 None:intergenic 35.0%
AGTAATTACTGAGACAGCCA+CGG - chr3.1:58233762-58233781 None:intergenic 40.0%
ATGGACAAAGCAGCATTACT+AGG + chr3.1:58233903-58233922 MS.gene82542:CDS 40.0%
GAACTAATCCAAGTCCTCAA+AGG + chr3.1:58234134-58234153 MS.gene82542:CDS 40.0%
GTCGTATTAGTACCTGAAAG+TGG - chr3.1:58233661-58233680 None:intergenic 40.0%
TCTTCAACACTCCCTTCAAT+AGG - chr3.1:58232891-58232910 None:intergenic 40.0%
TGGACAAAGCAGCATTACTA+GGG + chr3.1:58233904-58233923 MS.gene82542:CDS 40.0%
TTGATTTCCACTTCCCCTAT+TGG + chr3.1:58233495-58233514 MS.gene82542:intron 40.0%
! ATTTTCTCCAATAGGGGAAG+TGG - chr3.1:58233505-58233524 None:intergenic 40.0%
! GTCTTAAACCTTTGAGGACT+TGG - chr3.1:58234145-58234164 None:intergenic 40.0%
! TATCTTATTTCGTACCGGTG+TGG - chr3.1:58233589-58233608 None:intergenic 40.0%
! TTAAAGGTATGCCCACTTTC+AGG + chr3.1:58233646-58233665 MS.gene82542:intron 40.0%
AAAATGGTCTCCTACCACAC+CGG + chr3.1:58233572-58233591 MS.gene82542:intron 45.0%
AAAGCCGACATAGCAAGTGT+TGG + chr3.1:58234176-58234195 MS.gene82542:CDS 45.0%
ACTCGATCCATTCATGCATC+AGG + chr3.1:58233470-58233489 MS.gene82542:intron 45.0%
ATCTGTGTAGGTACAGTCAC+AGG - chr3.1:58232798-58232817 None:intergenic 45.0%
CAGAGAAAAGACGTAGAGAC+AGG + chr3.1:58232946-58232965 MS.gene82542:CDS 45.0%
CTCTGCCTCTTACTTCTTGT+AGG - chr3.1:58234332-58234351 None:intergenic 45.0%
CTCTGCTTGACTATGACTCT+TGG - chr3.1:58232931-58232950 None:intergenic 45.0%
! CACTTGGGTTTTCCCTAAGT+AGG - chr3.1:58233145-58233164 None:intergenic 45.0%
! TTATTTCGTACCGGTGTGGT+AGG - chr3.1:58233585-58233604 None:intergenic 45.0%
AAGTCGCGTTGCAGTTCTGT+CGG - chr3.1:58233840-58233859 None:intergenic 50.0%
ATTGTGGCTCCCCGTTATTC+TGG + chr3.1:58233440-58233459 MS.gene82542:intron 50.0%
GGCAGCATTTGAGCACAAAC+TGG + chr3.1:58232742-58232761 MS.gene82542:CDS 50.0%
!! GCCACCAACACTTGCTATGT+CGG - chr3.1:58234183-58234202 None:intergenic 50.0%
AGTCGCGTTGCAGTTCTGTC+GGG - chr3.1:58233839-58233858 None:intergenic 55.0%
!! GCCGACATAGCAAGTGTTGG+TGG + chr3.1:58234179-58234198 MS.gene82542:CDS 55.0%
! TGTTTGGTGAGCAGCGTCCG+TGG + chr3.1:58233742-58233761 MS.gene82542:intron 60.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 58232708 58234379 58232708 ID=MS.gene82542
chr3.1 mRNA 58232708 58234379 58232708 ID=MS.gene82542.t1;Parent=MS.gene82542
chr3.1 exon 58232708 58233019 58232708 ID=MS.gene82542.t1.exon1;Parent=MS.gene82542.t1
chr3.1 CDS 58232708 58233019 58232708 ID=cds.MS.gene82542.t1;Parent=MS.gene82542.t1
chr3.1 exon 58233903 58234379 58233903 ID=MS.gene82542.t1.exon2;Parent=MS.gene82542.t1
chr3.1 CDS 58233903 58234379 58233903 ID=cds.MS.gene82542.t1;Parent=MS.gene82542.t1
Gene Sequence

>MS.gene82542

ATGAGTATGGAAAACTACAATGATTCTTCTGAGTGGCAGCATTTGAGCACAAACTGGTCTCAATACAGTAATGATCATTCACAATTTCCTGTGACTGTACCTACACAGATACTACCTCATAATATCTCTGACTCTTCTTCTGGTTCTTTTCAATATAGTGAATTTCAGTCATGGAATTTACCTATTGAAGGGAGTGTTGAAGATAGAGCAGCAAGTGCTTCCAAGAGTCATAGTCAAGCAGAGAAAAGACGTAGAGACAGGATTAATACACAGCTTGCTAATCTTAGAAAACTTATTCCTAAGTCTGATAAGGTAAATCAATTTTGTTTTTTTTCATGTTTGTCACATTTAATTTAGTTATATAGTATAGTATTAGTGTATTACTATTTGAAGCTTAGACTTTGTTACATATTTCATGCAAGAAAATGGAATTACCTACTTAGGGAAAACCCAAGTGATAAGATGTTGCATTCATTCAATTCAGGCTTTGAAGAATAAGTGTGCTATTGTGATTTTGACAAAAGCTACCTTTTGGGACTTACATAAAATGTTGTTGGCACTGTAATTTTGTCAAACTCGTTATAATTCAAAGCATGTACTTGGTTGAAATTTCAATTGTAGTCTTGAGTATGAACAATTTGGTATCTATTTCTTATTGTGTGTTTGGATTGATGGTGAATTCAACAAAATCACGAAGACACTGATTTTGTGACACTAAAATTTTCTAGATTCATTGTGGCTCCCCGTTATTCTGGCAATTTTACTCGATCCATTCATGCATCAGGAATTGATTTCCACTTCCCCTATTGGAGAAAATATAGGATGAAACTTACATTATACACAAAACATAATTTTTTTTTTTTAAAAATGGTCTCCTACCACACCGGTACGAAATAAGATATTTGATGAGTCTATCTACGTAGCACTAATACTTCGTATTAAAGGTATGCCCACTTTCAGGTACTAATACGACACTGACACATGTGATTGCATTTAATCGCATCCATTTTCTCAAATTATTTTCAGTGTGTACATGTTTGGTGAGCAGCGTCCGTGGCTGTCTCAGTAATTACTAATTTACTACTACGCTAGTCGTATTTGATTACAATTTTCTGCAATATTAAGGATCCCGACAGAACTGCAACGCGACTTTGATTGAATCCCTTGCATAACATAATCATTTGTATACTTGCAGATGGACAAAGCAGCATTACTAGGGAGTGTGATAGATCAAGTCAAAGATCTAAAGCGAAAAGCAATGGATGTTAGCAGAGTCATCACAGTTCCAACTGAAATTGATGAAGTATCAATAGACTATAACCATGTTGTTGAAGATGAAACCAACACTAACAAAGTGAACAAATTCAAAGACAATATCATCATCAAAGCTTCTGTTTGTTGCGACGATCGACCAGAATTGTTCTCTGAACTAATCCAAGTCCTCAAAGGTTTAAGACTTACAACAGTTAAAGCCGACATAGCAAGTGTTGGTGGCAGAATCAAAAGTATATTGGTGCTTTGTTCTAAGGACAGTGAAGAAAATGTTTGCATTAATACTCTCAAACAGTCCCTTAAATCAGCAGTGACCAAAATTGCTTCATCATCAATGGTATCTAATTGTCCTACAAGAAGTAAGAGGCAGAGATTCTTTTTGCCTTCTCATTTTGTATAG

Protein sequence

>MS.gene82542.t1

MSMENYNDSSEWQHLSTNWSQYSNDHSQFPVTVPTQILPHNISDSSSGSFQYSEFQSWNLPIEGSVEDRAASASKSHSQAEKRRRDRINTQLANLRKLIPKSDKMDKAALLGSVIDQVKDLKRKAMDVSRVITVPTEIDEVSIDYNHVVEDETNTNKVNKFKDNIIIKASVCCDDRPELFSELIQVLKGLRLTTVKADIASVGGRIKSILVLCSKDSEENVCINTLKQSLKSAVTKIASSSMVSNCPTRSKRQRFFLPSHFV