AlfalfaGEDB | MS.gene21620

M. sativa cultivar XinJiangDaYe / MS.gene21620

KEGG: K09872
GO: GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0008150 GO:0015267 GO:0016020 GO:0022803 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0110165
PFAM: PF00230.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21620.t1	XP_003611079.1	99.3	287	2	0	1	287	1	287	3.10E-160	574.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21620.t1	Q7XSQ9	88.4	285	31	2	3	286	4	287	2.3e-143	509.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21620.t1	G7KC21	99.3	287	2	0	1	287	1	287	2.2e-160	574.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04985	MS.gene21620	0.806686	7.29E-50	-1.69E-46
MS.gene051263	MS.gene21620	0.807225	5.61E-50	-1.69E-46
MS.gene051478	MS.gene21620	0.803715	3.05E-49	-1.69E-46
MS.gene051781	MS.gene21620	0.824708	6.97E-54	-1.69E-46
MS.gene051784	MS.gene21620	0.809762	1.61E-50	-1.69E-46
MS.gene051787	MS.gene21620	0.822621	2.15E-53	-1.69E-46
MS.gene054999	MS.gene21620	0.801096	1.06E-48	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene21620	MS.gene006958	PPI
MS.gene21620	MS.gene03121	PPI
MS.gene21620	MS.gene93201	PPI
MS.gene072269	MS.gene21620	PPI
MS.gene27853	MS.gene21620	PPI
MS.gene004953	MS.gene21620	PPI
MS.gene21620	MS.gene006957	PPI
MS.gene025449	MS.gene21620	PPI
MS.gene21620	MS.gene54792	PPI
MS.gene21620	MS.gene67362	PPI
MS.gene067014	MS.gene21620	PPI
MS.gene49432	MS.gene21620	PPI
MS.gene21620	MS.gene067014	PPI
MS.gene21620	MS.gene36342	PPI
MS.gene85141	MS.gene21620	PPI
MS.gene61127	MS.gene21620	PPI
MS.gene32446	MS.gene21620	PPI
MS.gene36973	MS.gene21620	PPI
MS.gene72127	MS.gene21620	PPI
MS.gene31709	MS.gene21620	PPI
MS.gene89899	MS.gene21620	PPI
MS.gene71137	MS.gene21620	PPI
MS.gene34830	MS.gene21620	PPI
MS.gene019314	MS.gene21620	PPI
MS.gene21620	MS.gene048817	PPI
MS.gene27852	MS.gene21620	PPI
MS.gene006960	MS.gene21620	PPI
MS.gene21620	MS.gene069812	PPI
MS.gene21620	MS.gene79774	PPI
MS.gene72129	MS.gene21620	PPI
MS.gene021393	MS.gene21620	PPI
MS.gene21620	MS.gene01625	PPI
MS.gene025056	MS.gene21620	PPI
MS.gene03094	MS.gene21620	PPI
MS.gene062190	MS.gene21620	PPI
MS.gene21620	MS.gene065973	PPI
MS.gene21620	MS.gene004324	PPI
MS.gene21620	MS.gene004088	PPI
MS.gene065972	MS.gene21620	PPI
MS.gene21620	MS.gene03090	PPI
MS.gene21620	MS.gene052743	PPI
MS.gene022891	MS.gene21620	PPI
MS.gene21620	MS.gene059531	PPI
MS.gene006959	MS.gene21620	PPI
MS.gene072777	MS.gene21620	PPI
MS.gene21620	MS.gene33486	PPI
MS.gene03093	MS.gene21620	PPI
MS.gene21620	MS.gene059380	PPI
MS.gene21620	MS.gene023622	PPI
MS.gene024637	MS.gene21620	PPI
MS.gene21620	MS.gene89899	PPI
MS.gene21620	MS.gene037437	PPI
MS.gene21620	MS.gene002643	PPI
MS.gene014206	MS.gene21620	PPI
MS.gene34032	MS.gene21620	PPI
MS.gene021061	MS.gene21620	PPI
MS.gene07481	MS.gene21620	PPI
MS.gene01627	MS.gene21620	PPI
MS.gene21620	MS.gene01624	PPI
MS.gene21620	MS.gene069860	PPI
MS.gene32445	MS.gene21620	PPI
MS.gene21620	MS.gene023621	PPI
MS.gene069812	MS.gene21620	PPI
MS.gene21620	MS.gene042849	PPI
MS.gene005974	MS.gene21620	PPI
MS.gene059378	MS.gene21620	PPI
MS.gene21620	MS.gene002639	PPI
MS.gene021394	MS.gene21620	PPI
MS.gene21620	MS.gene004321	PPI
MS.gene21620	MS.gene006961	PPI
MS.gene059379	MS.gene21620	PPI
MS.gene002640	MS.gene21620	PPI
MS.gene76526	MS.gene21620	PPI
MS.gene21620	MS.gene36789	PPI
MS.gene004322	MS.gene21620	PPI
MS.gene83316	MS.gene21620	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21620.t1	MTR_5g010150	99.303	287	2	0	1	287	1	287	0.0	580
MS.gene21620.t1	MTR_8g098375	93.706	286	18	0	1	286	1	286	0.0	553
MS.gene21620.t1	MTR_4g415300	84.722	288	40	2	2	286	3	289	3.84e-180	498
MS.gene21620.t1	MTR_5g082070	84.965	286	41	1	3	286	4	289	6.87e-180	497
MS.gene21620.t1	MTR_3g070210	84.669	287	42	1	3	287	4	290	3.10e-179	495
MS.gene21620.t1	MTR_3g070210	84.170	259	39	1	3	259	4	262	4.24e-158	441
MS.gene21620.t1	MTR_5g010150	99.524	210	1	0	1	210	1	210	3.44e-153	427
MS.gene21620.t1	MTR_8g098375	86.900	229	28	1	1	227	1	229	9.64e-146	409
MS.gene21620.t1	MTR_7g101190	74.525	263	60	2	29	285	16	277	1.12e-140	398
MS.gene21620.t1	MTR_2g094270	68.889	270	76	2	23	285	11	279	7.66e-136	385
MS.gene21620.t1	MTR_4g059390	68.914	267	76	2	25	285	12	277	1.43e-131	375
MS.gene21620.t1	MTR_3g118010	69.057	265	81	1	21	285	6	269	2.90e-131	374
MS.gene21620.t1	MTR_3g070210	82.857	210	34	1	3	210	4	213	3.34e-124	353
MS.gene21620.t1	MTR_4g415300	81.604	212	35	2	2	210	3	213	4.00e-123	351
MS.gene21620.t1	MTR_2g094270	69.295	241	66	2	23	256	11	250	9.32e-120	344
MS.gene21620.t1	MTR_1g095070	76.316	190	44	1	96	285	23	211	1.56e-103	301
MS.gene21620.t1	MTR_1g095070	77.019	161	36	1	96	256	23	182	5.07e-87	258
MS.gene21620.t1	MTR_4g059390	66.667	192	57	2	25	210	12	202	1.68e-85	254
MS.gene21620.t1	MTR_1g077540	35.776	232	130	6	52	278	21	238	1.06e-32	121
MS.gene21620.t1	MTR_6g069040	39.834	241	127	8	52	287	21	248	1.24e-32	120
MS.gene21620.t1	MTR_2g101370	35.897	234	135	5	47	277	15	236	2.67e-32	120
MS.gene21620.t1	MTR_8g013680	36.752	234	131	6	46	274	11	232	6.12e-32	119
MS.gene21620.t1	MTR_1g115405	34.836	244	139	7	41	278	10	239	5.75e-29	111
MS.gene21620.t1	MTR_7g103030	33.745	243	137	7	42	277	11	236	4.79e-28	108
MS.gene21620.t1	MTR_7g023350	34.426	244	140	7	41	278	10	239	5.44e-28	108
MS.gene21620.t1	MTR_4g063090	32.773	238	145	5	41	275	10	235	1.27e-27	107
MS.gene21620.t1	MTR_1g006490	34.199	231	125	7	52	275	19	229	1.57e-27	107
MS.gene21620.t1	MTR_5g012810	37.168	226	126	6	52	274	19	231	1.45e-26	104
MS.gene21620.t1	MTR_1g006590	33.906	233	123	8	52	275	19	229	3.32e-26	103
MS.gene21620.t1	MTR_2g060360	32.365	241	125	9	52	278	19	235	2.29e-20	88.2
MS.gene21620.t1	MTR_5g079570	29.688	192	122	4	84	275	68	246	3.61e-19	85.1
MS.gene21620.t1	MTR_8g087710	29.289	239	145	7	43	277	30	248	8.16e-19	84.3
MS.gene21620.t1	MTR_2g017590	32.203	236	137	7	55	287	39	254	9.03e-19	84.3
MS.gene21620.t1	MTR_5g063930	27.632	228	150	5	47	274	32	244	1.24e-18	83.6
MS.gene21620.t1	MTR_4g084880	25.664	226	148	5	55	277	38	246	1.87e-18	83.2
MS.gene21620.t1	MTR_2g017660	28.634	227	146	6	41	267	28	238	5.40e-18	82.0
MS.gene21620.t1	MTR_3g008920	31.250	192	119	4	84	275	54	232	6.01e-18	81.6
MS.gene21620.t1	MTR_2g017620	30.705	241	144	7	50	287	22	242	1.11e-17	80.9
MS.gene21620.t1	MTR_2g017620	30.705	241	144	7	50	287	36	256	1.35e-17	80.9
MS.gene21620.t1	MTR_4g075570	28.205	195	118	7	99	279	95	281	4.18e-17	80.5
MS.gene21620.t1	MTR_4g075590	26.316	247	163	7	44	279	29	267	5.29e-17	80.1
MS.gene21620.t1	MTR_8g087720	27.197	239	157	6	49	287	35	256	6.92e-17	79.0
MS.gene21620.t1	MTR_2g017610	30.043	233	146	6	55	287	41	256	6.96e-17	79.0
MS.gene21620.t1	MTR_2g017570	29.614	233	147	6	55	287	41	256	1.58e-16	78.2
MS.gene21620.t1	MTR_4g006730	28.302	212	135	6	56	267	79	273	6.82e-16	76.6
MS.gene21620.t1	MTR_7g010650	27.966	236	150	7	50	284	35	251	6.92e-16	76.3
MS.gene21620.t1	MTR_2g104940	30.516	213	129	7	56	267	84	278	2.09e-15	75.5
MS.gene21620.t1	MTR_4g006630	29.609	179	112	4	89	267	22	186	5.42e-14	70.1
MS.gene21620.t1	MTR_4g006730	29.609	179	112	4	89	267	22	186	5.69e-14	69.7
MS.gene21620.t1	MTR_2g017620	31.053	190	111	5	101	287	3	175	1.13e-13	68.6
MS.gene21620.t1	MTR_1g097840	28.319	226	142	8	56	279	78	285	7.92e-13	67.8
MS.gene21620.t1	MTR_1g070385	26.872	227	143	7	49	272	59	265	6.13e-12	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21620.t1	AT4G00430	86.667	285	37	1	3	287	4	287	0.0	507
MS.gene21620.t1	AT2G45960	85.965	285	38	2	3	287	4	286	0.0	499
MS.gene21620.t1	AT4G23400	87.324	284	35	1	3	286	4	286	1.12e-175	486
MS.gene21620.t1	AT1G01620	87.018	285	35	2	3	287	4	286	1.97e-174	483
MS.gene21620.t1	AT3G61430	85.614	285	39	2	3	287	4	286	3.67e-172	477
MS.gene21620.t1	AT3G61430	85.614	285	39	2	3	287	4	286	3.67e-172	477
MS.gene21620.t1	AT2G45960	85.827	254	34	2	3	256	4	255	1.46e-159	445
MS.gene21620.t1	AT2G45960	85.827	254	34	2	3	256	4	255	4.39e-158	442
MS.gene21620.t1	AT1G01620	89.302	215	22	1	73	287	1	214	1.44e-138	389
MS.gene21620.t1	AT2G37170	72.692	260	64	3	27	280	55	313	4.41e-135	385
MS.gene21620.t1	AT2G37170	72.692	260	64	3	27	280	12	270	1.12e-134	382
MS.gene21620.t1	AT4G35100	71.483	263	74	1	23	285	9	270	1.73e-134	382
MS.gene21620.t1	AT4G35100	71.483	263	74	1	23	285	9	270	1.73e-134	382
MS.gene21620.t1	AT3G53420	72.692	260	64	2	27	280	14	272	2.09e-134	382
MS.gene21620.t1	AT3G53420	72.692	260	64	2	27	280	14	272	2.09e-134	382
MS.gene21620.t1	AT3G54820	68.000	275	81	2	17	285	3	276	3.42e-134	381
MS.gene21620.t1	AT2G39010	68.364	275	80	2	17	285	3	276	2.72e-133	379
MS.gene21620.t1	AT2G16850	72.374	257	70	1	29	285	13	268	5.59e-133	378
MS.gene21620.t1	AT2G37180	71.538	260	67	3	27	280	12	270	1.11e-132	377
MS.gene21620.t1	AT5G60660	70.455	264	71	2	28	285	15	277	2.11e-132	377
MS.gene21620.t1	AT4G00430	83.019	212	35	1	3	214	4	214	2.85e-126	358
MS.gene21620.t1	AT2G39010	64.500	200	64	2	17	210	3	201	7.96e-85	254
MS.gene21620.t1	AT5G47450	37.872	235	131	6	43	274	10	232	4.66e-31	116
MS.gene21620.t1	AT1G73190	36.066	244	127	8	52	282	24	251	5.34e-31	117
MS.gene21620.t1	AT4G01470	34.711	242	139	6	41	277	10	237	1.22e-30	115
MS.gene21620.t1	AT3G16240	36.364	231	132	5	47	274	14	232	1.63e-30	115
MS.gene21620.t1	AT1G17810	36.170	235	130	5	52	278	24	246	5.23e-30	114
MS.gene21620.t1	AT2G36830	34.286	245	134	9	41	276	10	236	5.52e-28	108
MS.gene21620.t1	AT3G26520	33.188	229	138	5	52	277	22	238	1.29e-27	107
MS.gene21620.t1	AT4G17340	36.596	235	134	6	43	274	10	232	5.03e-27	106
MS.gene21620.t1	AT3G47440	32.759	232	136	6	52	278	23	239	4.33e-25	101
MS.gene21620.t1	AT2G25810	34.335	233	136	8	52	282	19	236	9.15e-25	100
MS.gene21620.t1	AT4G19030	29.643	280	171	9	3	277	13	271	1.41e-19	87.0
MS.gene21620.t1	AT1G31885	32.000	225	140	7	45	267	36	249	5.51e-19	85.5
MS.gene21620.t1	AT4G18910	28.512	242	162	6	38	277	36	268	6.64e-17	79.3
MS.gene21620.t1	AT5G37820	28.111	217	130	7	55	266	46	241	8.97e-17	79.0
MS.gene21620.t1	AT5G37820	28.111	217	130	7	55	266	82	277	1.40e-16	79.0
MS.gene21620.t1	AT2G34390	26.119	268	168	9	16	277	20	263	6.01e-16	76.6
MS.gene21620.t1	AT1G31885	31.892	185	116	5	85	267	19	195	9.14e-16	75.9
MS.gene21620.t1	AT2G34390	27.511	229	144	8	55	277	9	221	4.19e-14	70.9
MS.gene21620.t1	AT3G06100	29.707	239	140	8	52	286	46	260	1.25e-13	69.7
MS.gene21620.t1	AT3G06100	29.661	236	138	8	55	286	1	212	3.27e-13	68.2
MS.gene21620.t1	AT4G10380	26.316	266	160	9	20	267	29	276	5.73e-13	68.2
MS.gene21620.t1	AT1G80760	29.004	231	143	8	49	277	77	288	9.48e-13	67.8
MS.gene21620.t1	AT5G37810	23.789	227	149	6	55	277	46	252	2.04e-12	66.6

Find 71 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAAGGAATTGCTTGGGCTTT+TGG	0.161774	5.2:-3515240	MS.gene21620:CDS
TATTGCACTGCTGGAATTTC+AGG	0.165208	5.2:-3515195	MS.gene21620:intron
TTGGCTACAATTCCCATTAC+TGG	0.294571	5.2:-3514312	MS.gene21620:CDS
TTCTGGGTTGGACCTTTCAT+TGG	0.308099	5.2:-3514109	MS.gene21620:CDS
GCCCTTGTCTATTGCACTGC+TGG	0.332113	5.2:-3515204	MS.gene21620:CDS
ACCAACAGAGGCACACTTAG+AGG	0.335616	5.2:+3515266	None:intergenic
GGAGAGGGAGGAAGATGTTA+AGG	0.347098	5.2:-3515499	MS.gene21620:CDS
GATCCGAGCAATTCCTTTCA+AGG	0.393867	5.2:-3514053	MS.gene21620:CDS
TTGGTATTCAAGGAATTGCT+TGG	0.400234	5.2:-3515248	MS.gene21620:CDS
GCTCGTTATGAGTTGTATAA+AGG	0.401946	5.2:-3514671	MS.gene21620:CDS
TGTGCCTCTGTTGGTATTCA+AGG	0.404573	5.2:-3515258	MS.gene21620:CDS
ACAATTCCCATTACTGGAAC+TGG	0.407279	5.2:-3514306	MS.gene21620:CDS
GAATTGCTCGGATCACTATC+TGG	0.408932	5.2:+3514062	None:intergenic
ATGGTCTCTGTTGTATATTA+TGG	0.412666	5.2:+3514254	None:intergenic
AGAGTCTCTTGCATTTCTCT+TGG	0.418783	5.2:+3514544	None:intergenic
AGGGAGGAAGATGTTAAGGT+TGG	0.422571	5.2:-3515495	MS.gene21620:CDS
TGCAAGAGACTCTCACGTTC+CGG	0.436085	5.2:-3514534	MS.gene21620:intron
TGGTATTCAAGGAATTGCTT+GGG	0.437803	5.2:-3515247	MS.gene21620:CDS
TACACCAAGGGTGATGGTCT+TGG	0.441240	5.2:-3514623	MS.gene21620:CDS
CTTGGAGCTATCTGTGGTGC+TGG	0.453990	5.2:-3514719	MS.gene21620:CDS
TTCTCAGAAAAGAATGCATT+GGG	0.454430	5.2:-3515462	MS.gene21620:CDS
AATTATTCCCTTGCCTTGAA+AGG	0.458161	5.2:+3514040	None:intergenic
CCAGCACCTTTGTTTGAGCC+AGG	0.466057	5.2:-3515396	MS.gene21620:CDS
CCCTCTAAGTGTGCCTCTGT+TGG	0.469372	5.2:-3515267	MS.gene21620:CDS
TTAATGCCAGTTCCAGTAAT+GGG	0.469401	5.2:+3514300	None:intergenic
ATAAATCCAGCGGTGACATT+CGG	0.475431	5.2:-3514803	MS.gene21620:CDS
TACATAATAATGCAGTGTCT+TGG	0.479630	5.2:-3514737	MS.gene21620:CDS
GACCATGACTTCAACTCTCC+TGG	0.486464	5.2:+3515378	None:intergenic
AACTCTCCTGGCTCAAACAA+AGG	0.498554	5.2:+3515390	None:intergenic
CATCACTGTCTTAACTGTGA+TGG	0.500492	5.2:-3515304	MS.gene21620:CDS
GTTCTCAGAAAAGAATGCAT+TGG	0.502108	5.2:-3515463	MS.gene21620:CDS
GGTCTTGGAGCTGAGATTGT+TGG	0.502760	5.2:-3514608	MS.gene21620:CDS
CCTGGCTCAAACAAAGGTGC+TGG	0.505994	5.2:+3515396	None:intergenic
TGCATTTCTCTTGGCATCAG+TGG	0.515764	5.2:+3514553	None:intergenic
TAACTGTGATGGGTGTGAAT+AGG	0.515829	5.2:-3515293	MS.gene21620:CDS
GAAAAGAATGCATTGGGCAC+AGG	0.516964	5.2:-3515456	MS.gene21620:CDS
AGCAATTCCTTTCAAGGCAA+GGG	0.517229	5.2:-3514047	MS.gene21620:CDS
ATTAACCCAGCTAGGAGTCT+TGG	0.521008	5.2:-3514282	MS.gene21620:CDS
TATACAACAGAGACCATGCT+TGG	0.521813	5.2:-3514248	MS.gene21620:CDS
AGCTCCAAGACCATCACCCT+TGG	0.522666	5.2:+3514619	None:intergenic
GAGTGATAGCAAGGACTACA+AGG	0.528511	5.2:-3515427	MS.gene21620:CDS
GCAGCACCAAGACTCCTAGC+TGG	0.528907	5.2:+3514276	None:intergenic
CCATCACCCTTGGTGTAACC+AGG	0.529590	5.2:+3514629	None:intergenic
CAGTTAAGACAGTGATGTAA+AGG	0.533154	5.2:+3515310	None:intergenic
TTGCCTTGAAAGGAATTGCT+CGG	0.539077	5.2:+3514050	None:intergenic
AACAAACCGAATGTCACCGC+TGG	0.543714	5.2:+3514797	None:intergenic
ATCACTGTCTTAACTGTGAT+GGG	0.546772	5.2:-3515303	MS.gene21620:CDS
GCAAGTGCAGCTCCAATGAA+AGG	0.549715	5.2:+3514097	None:intergenic
GGAGCAAATGTTGTGAATCC+TGG	0.560797	5.2:-3514647	MS.gene21620:CDS
AGGAGCTCAGAGTGATAGCA+AGG	0.561366	5.2:-3515436	MS.gene21620:CDS
GGCTCAAACAAAGGTGCTGG+TGG	0.561806	5.2:+3515399	None:intergenic
GTTAATGCCAGTTCCAGTAA+TGG	0.566105	5.2:+3514299	None:intergenic
CCTGGTTACACCAAGGGTGA+TGG	0.571890	5.2:-3514629	MS.gene21620:CDS
GAGCAATTCCTTTCAAGGCA+AGG	0.579031	5.2:-3514048	MS.gene21620:CDS
CGTTATGAGTTGTATAAAGG+TGG	0.591045	5.2:-3514668	MS.gene21620:CDS
CAGTGTCTTGGAGCTATCTG+TGG	0.593126	5.2:-3514725	MS.gene21620:CDS
ACAGCAAACCCAATTGGAAG+AGG	0.593192	5.2:+3514348	None:intergenic
CAGCACCAAGACTCCTAGCT+GGG	0.594486	5.2:+3514277	None:intergenic
GTAGAATACTGCTCTTGTCA+AGG	0.594791	5.2:+3514757	None:intergenic
ATACAACAGAGACCATGCTT+GGG	0.595986	5.2:-3514247	MS.gene21620:CDS
AGCCAGGAGAGTTGAAGTCA+TGG	0.598254	5.2:-3515380	MS.gene21620:CDS
CCAACAGAGGCACACTTAGA+GGG	0.603003	5.2:+3515267	None:intergenic
TTCCAGCAGTGCAATAGACA+AGG	0.608111	5.2:+3515202	None:intergenic
TGTGGTGCTGGTGTAGTGAA+AGG	0.611215	5.2:-3514707	MS.gene21620:CDS
ATACATGGTCATCCCAAGCA+TGG	0.639779	5.2:+3514235	None:intergenic
GAACTGGCATTAACCCAGCT+AGG	0.641090	5.2:-3514290	MS.gene21620:CDS
TCCAGCAGTGCAATAGACAA+GGG	0.662213	5.2:+3515203	None:intergenic
GTGAATCCTGGTTACACCAA+GGG	0.663817	5.2:-3514635	MS.gene21620:CDS
AATTCCTTGAATACCAACAG+AGG	0.711099	5.2:+3515254	None:intergenic
AGGTGGGCACATAAATCCAG+CGG	0.718916	5.2:-3514813	MS.gene21620:CDS
TGTGAATCCTGGTTACACCA+AGG	0.762851	5.2:-3514636	MS.gene21620:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CAGAACAAAAAATAAATTGA+AGG	+	chr5.2:3515362-3515381	None:intergenic	20.0%
!!!	ATGTTTGTATTTGTAGATTT+TGG	-	chr5.2:3515170-3515189	MS.gene21620:intron	20.0%
!	AAGAGATTAATATGATGTGA+GGG	-	chr5.2:3514618-3514637	MS.gene21620:CDS	25.0%
!	TAAGAGATTAATATGATGTG+AGG	-	chr5.2:3514617-3514636	MS.gene21620:CDS	25.0%
!	TCTCATGATTCATAAAATTG+TGG	+	chr5.2:3514410-3514429	None:intergenic	25.0%
!!!	AAATTAAACAGTGGATTTTC+TGG	-	chr5.2:3515417-3515436	MS.gene21620:CDS	25.0%
!!!	AATTAAACAGTGGATTTTCT+GGG	-	chr5.2:3515418-3515437	MS.gene21620:CDS	25.0%
!!!	TGATATATGCTTCATTTTTG+TGG	-	chr5.2:3515131-3515150	MS.gene21620:intron	25.0%
!!!	TGTTTAATGTTTGAGTTTTG+TGG	-	chr5.2:3515393-3515412	MS.gene21620:CDS	25.0%
	ATGGTCTCTGTTGTATATTA+TGG	+	chr5.2:3515292-3515311	None:intergenic	30.0%
	GACAAGTCTTTAATCTGAAA+TGG	-	chr5.2:3514534-3514553	MS.gene21620:intron	30.0%
	TACATAATAATGCAGTGTCT+TGG	-	chr5.2:3514806-3514825	MS.gene21620:CDS	30.0%
	TGAAATTCATTTACACGTAC+CGG	+	chr5.2:3515031-3515050	None:intergenic	30.0%
	TTCTCAGAAAAGAATGCATT+GGG	-	chr5.2:3514081-3514100	MS.gene21620:CDS	30.0%
	TTTCAGATTAAAGACTTGTC+CGG	+	chr5.2:3514534-3514553	None:intergenic	30.0%
	TTTGAACAAAACTAACGAGA+TGG	-	chr5.2:3514699-3514718	MS.gene21620:CDS	30.0%
!	AAGTCTTTAATCTGAAATGG+TGG	-	chr5.2:3514537-3514556	MS.gene21620:CDS	30.0%
!	TTTAATCTGAAATGGTGGTT+TGG	-	chr5.2:3514542-3514561	MS.gene21620:CDS	30.0%
!	TTTTGTGGCAAATTAAACAG+TGG	-	chr5.2:3515408-3515427	MS.gene21620:CDS	30.0%
	AAAAACACAGCAAACCCAAT+TGG	+	chr5.2:3515204-3515223	None:intergenic	35.0%
	AACAAAACTAACGAGATGGT+TGG	-	chr5.2:3514703-3514722	MS.gene21620:CDS	35.0%
	AATTCCTTGAATACCAACAG+AGG	+	chr5.2:3514292-3514311	None:intergenic	35.0%
	ACAAAACTAACGAGATGGTT+GGG	-	chr5.2:3514704-3514723	MS.gene21620:CDS	35.0%
	ATCACTGTCTTAACTGTGAT+GGG	-	chr5.2:3514240-3514259	MS.gene21620:CDS	35.0%
	ATGATGTGAGGGACTAAATT+AGG	-	chr5.2:3514629-3514648	MS.gene21620:CDS	35.0%
	CAGTTAAGACAGTGATGTAA+AGG	+	chr5.2:3514236-3514255	None:intergenic	35.0%
	CGTTATGAGTTGTATAAAGG+TGG	-	chr5.2:3514875-3514894	MS.gene21620:intron	35.0%
	GCTCGTTATGAGTTGTATAA+AGG	-	chr5.2:3514872-3514891	MS.gene21620:intron	35.0%
	GTCAGTATTAGTGTCGTATT+GGG	+	chr5.2:3514439-3514458	None:intergenic	35.0%
	GTTCTCAGAAAAGAATGCAT+TGG	-	chr5.2:3514080-3514099	MS.gene21620:CDS	35.0%
	TCAATCTGTCACATAAACAC+CGG	-	chr5.2:3514512-3514531	MS.gene21620:intron	35.0%
	TGATGTGAGGGACTAAATTA+GGG	-	chr5.2:3514630-3514649	MS.gene21620:CDS	35.0%
	TGGTATTCAAGGAATTGCTT+GGG	-	chr5.2:3514296-3514315	MS.gene21620:CDS	35.0%
	TGTCAGTATTAGTGTCGTAT+TGG	+	chr5.2:3514440-3514459	None:intergenic	35.0%
	TTAATGCCAGTTCCAGTAAT+GGG	+	chr5.2:3515246-3515265	None:intergenic	35.0%
	TTGGTATTCAAGGAATTGCT+TGG	-	chr5.2:3514295-3514314	MS.gene21620:CDS	35.0%
!!	CATGGTCATTTTATAGAGCT+GGG	-	chr5.2:3514181-3514200	MS.gene21620:intron	35.0%
!!	TCATGGTCATTTTATAGAGC+TGG	-	chr5.2:3514180-3514199	MS.gene21620:intron	35.0%
!!	TGCTTCATTTTTGTGGACTA+AGG	-	chr5.2:3515138-3515157	MS.gene21620:intron	35.0%
!!!	AATTGGGTTTGCTGTGTTTT+TGG	-	chr5.2:3515203-3515222	MS.gene21620:CDS	35.0%
	ACAAAGTGGAAGGACATACA+TGG	+	chr5.2:3515326-3515345	None:intergenic	40.0%
	ACAATTCCCATTACTGGAAC+TGG	-	chr5.2:3515237-3515256	MS.gene21620:CDS	40.0%
	AGAGTCTCTTGCATTTCTCT+TGG	+	chr5.2:3515002-3515021	None:intergenic	40.0%
	AGCAATTCCTTTCAAGGCAA+GGG	-	chr5.2:3515496-3515515	MS.gene21620:CDS	40.0%
	ATAAATCCAGCGGTGACATT+CGG	-	chr5.2:3514740-3514759	MS.gene21620:CDS	40.0%
	ATACAACAGAGACCATGCTT+GGG	-	chr5.2:3515296-3515315	MS.gene21620:CDS	40.0%
	CAACAGCATAACAAAGTGGA+AGG	+	chr5.2:3515336-3515355	None:intergenic	40.0%
	CATCACTGTCTTAACTGTGA+TGG	-	chr5.2:3514239-3514258	MS.gene21620:CDS	40.0%
	GCATCACCATCTTGAAATTG+TGG	+	chr5.2:3514659-3514678	None:intergenic	40.0%
	GTAGAATACTGCTCTTGTCA+AGG	+	chr5.2:3514789-3514808	None:intergenic	40.0%
	GTTAATGCCAGTTCCAGTAA+TGG	+	chr5.2:3515247-3515266	None:intergenic	40.0%
	TATACAACAGAGACCATGCT+TGG	-	chr5.2:3515295-3515314	MS.gene21620:CDS	40.0%
	TATTGCACTGCTGGAATTTC+AGG	-	chr5.2:3514348-3514367	MS.gene21620:CDS	40.0%
	TTGCCTTGAAAGGAATTGCT+CGG	+	chr5.2:3515496-3515515	None:intergenic	40.0%
	TTGGCTACAATTCCCATTAC+TGG	-	chr5.2:3515231-3515250	MS.gene21620:CDS	40.0%
!	TCCACTTTGTTATGCTGTTG+TGG	-	chr5.2:3515336-3515355	MS.gene21620:CDS	40.0%
!	TGGTGTAGTGAAAGGTTTTG+AGG	-	chr5.2:3514844-3514863	MS.gene21620:intron	40.0%
!	TTTTGGCACCTCTTCCAATT+GGG	-	chr5.2:3515187-3515206	MS.gene21620:intron	40.0%
!!	AAACAGTGGATTTTCTGGGT+TGG	-	chr5.2:3515422-3515441	MS.gene21620:CDS	40.0%
!!	ATTTTGGCACCTCTTCCAAT+TGG	-	chr5.2:3515186-3515205	MS.gene21620:intron	40.0%
!!	GGTGACATTCGGTTTGTTTT+TGG	-	chr5.2:3514751-3514770	MS.gene21620:CDS	40.0%
!!	GGTGTAGTGAAAGGTTTTGA+GGG	-	chr5.2:3514845-3514864	MS.gene21620:intron	40.0%
!!	TAACTGTGATGGGTGTGAAT+AGG	-	chr5.2:3514250-3514269	MS.gene21620:CDS	40.0%
!!!	CATTCGGTTTGTTTTTGGCT+AGG	-	chr5.2:3514756-3514775	MS.gene21620:CDS	40.0%
!!!	TGCTGTGTTTTTGGTTCACT+TGG	-	chr5.2:3515212-3515231	MS.gene21620:CDS	40.0%
	AACTCTCCTGGCTCAAACAA+AGG	+	chr5.2:3514156-3514175	None:intergenic	45.0%
	ACAGCAAACCCAATTGGAAG+AGG	+	chr5.2:3515198-3515217	None:intergenic	45.0%
	AGGGAGGAAGATGTTAAGGT+TGG	-	chr5.2:3514048-3514067	MS.gene21620:CDS	45.0%
	ATACATGGTCATCCCAAGCA+TGG	+	chr5.2:3515311-3515330	None:intergenic	45.0%
	ATTAACCCAGCTAGGAGTCT+TGG	-	chr5.2:3515261-3515280	MS.gene21620:CDS	45.0%
	GAAAAGAATGCATTGGGCAC+AGG	-	chr5.2:3514087-3514106	MS.gene21620:CDS	45.0%
	GAATTGCTCGGATCACTATC+TGG	+	chr5.2:3515484-3515503	None:intergenic	45.0%
	GAGCAATTCCTTTCAAGGCA+AGG	-	chr5.2:3515495-3515514	MS.gene21620:CDS	45.0%
	GAGTGATAGCAAGGACTACA+AGG	-	chr5.2:3514116-3514135	MS.gene21620:CDS	45.0%
	GATCCGAGCAATTCCTTTCA+AGG	-	chr5.2:3515490-3515509	MS.gene21620:CDS	45.0%
	GCCACAACAGCATAACAAAG+TGG	+	chr5.2:3515340-3515359	None:intergenic	45.0%
	GGAGCAAATGTTGTGAATCC+TGG	-	chr5.2:3514896-3514915	MS.gene21620:intron	45.0%
	GGGACTCCACAATTTCAAGA+TGG	-	chr5.2:3514650-3514669	MS.gene21620:CDS	45.0%
	GTGAATCCTGGTTACACCAA+GGG	-	chr5.2:3514908-3514927	MS.gene21620:intron	45.0%
	TCCAGCAGTGCAATAGACAA+GGG	+	chr5.2:3514343-3514362	None:intergenic	45.0%
	TGCATTTCTCTTGGCATCAG+TGG	+	chr5.2:3514993-3515012	None:intergenic	45.0%
	TGTGAATCCTGGTTACACCA+AGG	-	chr5.2:3514907-3514926	MS.gene21620:intron	45.0%
	TGTGCCTCTGTTGGTATTCA+AGG	-	chr5.2:3514285-3514304	MS.gene21620:CDS	45.0%
	TTCCAGCAGTGCAATAGACA+AGG	+	chr5.2:3514344-3514363	None:intergenic	45.0%
!	CAAGGAATTGCTTGGGCTTT+TGG	-	chr5.2:3514303-3514322	MS.gene21620:CDS	45.0%
!	TTCTGGGTTGGACCTTTCAT+TGG	-	chr5.2:3515434-3515453	MS.gene21620:CDS	45.0%
	AACAAACCGAATGTCACCGC+TGG	+	chr5.2:3514749-3514768	None:intergenic	50.0%
	AGCCAGGAGAGTTGAAGTCA+TGG	-	chr5.2:3514163-3514182	MS.gene21620:intron	50.0%
	AGGAGCTCAGAGTGATAGCA+AGG	-	chr5.2:3514107-3514126	MS.gene21620:CDS	50.0%
	AGGTGGGCACATAAATCCAG+CGG	-	chr5.2:3514730-3514749	MS.gene21620:CDS	50.0%
	CAGTGTCTTGGAGCTATCTG+TGG	-	chr5.2:3514818-3514837	MS.gene21620:intron	50.0%
	CTAACGAGATGGTTGGGAAC+AGG	-	chr5.2:3514710-3514729	MS.gene21620:CDS	50.0%
	GAACTGGCATTAACCCAGCT+AGG	-	chr5.2:3515253-3515272	MS.gene21620:CDS	50.0%
	GACCATGACTTCAACTCTCC+TGG	+	chr5.2:3514168-3514187	None:intergenic	50.0%
	GCAAGTGCAGCTCCAATGAA+AGG	+	chr5.2:3515449-3515468	None:intergenic	50.0%
	GGAGAGGGAGGAAGATGTTA+AGG	-	chr5.2:3514044-3514063	MS.gene21620:CDS	50.0%
	GGTCTTGGAGCTGAGATTGT+TGG	-	chr5.2:3514935-3514954	MS.gene21620:intron	50.0%
	TGCAAGAGACTCTCACGTTC+CGG	-	chr5.2:3515009-3515028	MS.gene21620:intron	50.0%
!	ACCAACAGAGGCACACTTAG+AGG	+	chr5.2:3514280-3514299	None:intergenic	50.0%
!	CCAACAGAGGCACACTTAGA+GGG	+	chr5.2:3514279-3514298	None:intergenic	50.0%
!	GGAATTGCTTGGGCTTTTGG+TGG	-	chr5.2:3514306-3514325	MS.gene21620:CDS	50.0%
!	TACACCAAGGGTGATGGTCT+TGG	-	chr5.2:3514920-3514939	MS.gene21620:intron	50.0%
!!	TGTGGTGCTGGTGTAGTGAA+AGG	-	chr5.2:3514836-3514855	MS.gene21620:intron	50.0%
	ACGAGATGGTTGGGAACAGG+TGG	-	chr5.2:3514713-3514732	MS.gene21620:CDS	55.0%
	AGCTCCAAGACCATCACCCT+TGG	+	chr5.2:3514927-3514946	None:intergenic	55.0%
	CAGCACCAAGACTCCTAGCT+GGG	+	chr5.2:3515269-3515288	None:intergenic	55.0%
	CCCTCTAAGTGTGCCTCTGT+TGG	-	chr5.2:3514276-3514295	MS.gene21620:CDS	55.0%
	CCTGGTTACACCAAGGGTGA+TGG	-	chr5.2:3514914-3514933	MS.gene21620:intron	55.0%
	CGAGATGGTTGGGAACAGGT+GGG	-	chr5.2:3514714-3514733	MS.gene21620:CDS	55.0%
	CTTGGAGCTATCTGTGGTGC+TGG	-	chr5.2:3514824-3514843	MS.gene21620:intron	55.0%
	GCCCTTGTCTATTGCACTGC+TGG	-	chr5.2:3514339-3514358	MS.gene21620:CDS	55.0%
!	CCAGCACCTTTGTTTGAGCC+AGG	-	chr5.2:3514147-3514166	MS.gene21620:intron	55.0%
!	CCATCACCCTTGGTGTAACC+AGG	+	chr5.2:3514917-3514936	None:intergenic	55.0%
!	CCTGGCTCAAACAAAGGTGC+TGG	+	chr5.2:3514150-3514169	None:intergenic	55.0%
!!	GGCTCAAACAAAGGTGCTGG+TGG	+	chr5.2:3514147-3514166	None:intergenic	55.0%
	GCAGCACCAAGACTCCTAGC+TGG	+	chr5.2:3515270-3515289	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	3514042	3515523	3514042	ID=MS.gene21620
chr5.2	mRNA	3514042	3515523	3514042	ID=MS.gene21620.t1;Parent=MS.gene21620
chr5.2	exon	3515196	3515523	3515196	ID=MS.gene21620.t1.exon1;Parent=MS.gene21620.t1
chr5.2	CDS	3515196	3515523	3515196	ID=cds.MS.gene21620.t1;Parent=MS.gene21620.t1
chr5.2	exon	3514535	3514833	3514535	ID=MS.gene21620.t1.exon2;Parent=MS.gene21620.t1
chr5.2	CDS	3514535	3514833	3514535	ID=cds.MS.gene21620.t1;Parent=MS.gene21620.t1
chr5.2	exon	3514239	3514379	3514239	ID=MS.gene21620.t1.exon3;Parent=MS.gene21620.t1
chr5.2	CDS	3514239	3514379	3514239	ID=cds.MS.gene21620.t1;Parent=MS.gene21620.t1
chr5.2	exon	3514042	3514137	3514042	ID=MS.gene21620.t1.exon4;Parent=MS.gene21620.t1
chr5.2	CDS	3514042	3514137	3514042	ID=cds.MS.gene21620.t1;Parent=MS.gene21620.t1

Gene Sequence

>MS.gene21620

ATGGAGAGGGAGGAAGATGTTAAGGTTGGAGCAAACAAGTTCTCAGAAAAGAATGCATTGGGCACAGGAGCTCAGAGTGATAGCAAGGACTACAAGGAAGCACCACCAGCACCTTTGTTTGAGCCAGGAGAGTTGAAGTCATGGTCATTTTATAGAGCTGGGATTGCTGAGTTTGTTGCAACTTTCTTGTTCCTTTACATCACTGTCTTAACTGTGATGGGTGTGAATAGGTCACCCTCTAAGTGTGCCTCTGTTGGTATTCAAGGAATTGCTTGGGCTTTTGGTGGTATGATCTTTGCCCTTGTCTATTGCACTGCTGGAATTTCAGGTAACTTCATTTTCTTTTCTTTACTTTTTTACTTGCTCCACAATTTTATGAATCATGAGACTTACACCCAATACGACACTAATACTGACACGTCGACATCAATAATAATATGAAAATGAGTAATTTAATGTAATCGCATGTTTCAATCTGTCACATAAACACCGGACAAGTCTTTAATCTGAAATGGTGGTTTGGTATGTAGATTCATCGTAAAATATTATAAATAGTATATGATATTTTGTAAATTTAAGAGATTAATATGATGTGAGGGACTAAATTAGGGACTCCACAATTTCAAGATGGTGATGCACTATAAATTATTATTTTGCTTTGAACAAAACTAACGAGATGGTTGGGAACAGGTGGGCACATAAATCCAGCGGTGACATTCGGTTTGTTTTTGGCTAGGAAGCTGTCCTTGACAAGAGCAGTATTCTACATAATAATGCAGTGTCTTGGAGCTATCTGTGGTGCTGGTGTAGTGAAAGGTTTTGAGGGTAATGCTCGTTATGAGTTGTATAAAGGTGGAGCAAATGTTGTGAATCCTGGTTACACCAAGGGTGATGGTCTTGGAGCTGAGATTGTTGGTACTTTTGTTCTTGTCTACACTGTCTTCTCTGCCACTGATGCCAAGAGAAATGCAAGAGACTCTCACGTTCCGGTACGTGTAAATGAATTTCAACTAATAAAAATAGTGTCGTAAGATGAATATTTGTTTATAATGAAAAGTGTGTTAAAGAGTTGTATTTCTAGCATCTCTATGATATATGCTTCATTTTTGTGGACTAAGGAGTAATGTGATGTTTGTATTTGTAGATTTTGGCACCTCTTCCAATTGGGTTTGCTGTGTTTTTGGTTCACTTGGCTACAATTCCCATTACTGGAACTGGCATTAACCCAGCTAGGAGTCTTGGTGCTGCCATAATATACAACAGAGACCATGCTTGGGATGACCATGTATGTCCTTCCACTTTGTTATGCTGTTGTGGCCTTCAATTTATTTTTTGTTCTGCTTAAGTGACTTGTTTAATGTTTGAGTTTTGTGGCAAATTAAACAGTGGATTTTCTGGGTTGGACCTTTCATTGGAGCTGCACTTGCTGCATTATATCACCAGATAGTGATCCGAGCAATTCCTTTCAAGGCAAGGGAATAA

Protein sequence

>MS.gene21620.t1

MEREEDVKVGANKFSEKNALGTGAQSDSKDYKEAPPAPLFEPGELKSWSFYRAGIAEFVATFLFLYITVLTVMGVNRSPSKCASVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAVFYIIMQCLGAICGAGVVKGFEGNARYELYKGGANVVNPGYTKGDGLGAEIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIIYNRDHAWDDHWIFWVGPFIGAALAALYHQIVIRAIPFKARE