AlfalfaGEDB | MS.gene014206

M. sativa cultivar XinJiangDaYe / MS.gene014206

KEGG: K09872
GO: GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0008150 GO:0015267 GO:0016020 GO:0022803 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0110165
PFAM: PF00230.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014206.t1	GAU33646.1	96.5	283	5	2	1	278	1	283	1.30E-150	542.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014206.t1	Q9ZVX8	89.7	282	21	2	1	278	1	278	3.0e-140	499.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014206.t1	A0A2Z6MMA4	96.5	283	5	2	1	278	1	283	9.2e-151	542.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene014206	MS.gene72129	PPI
MS.gene014206	MS.gene006961	PPI
MS.gene005974	MS.gene014206	PPI
MS.gene059378	MS.gene014206	PPI
MS.gene021394	MS.gene014206	PPI
MS.gene014206	MS.gene36789	PPI
MS.gene014206	MS.gene006958	PPI
MS.gene059379	MS.gene014206	PPI
MS.gene76526	MS.gene014206	PPI
MS.gene014206	MS.gene93201	PPI
MS.gene36973	MS.gene014206	PPI
MS.gene014206	MS.gene024637	PPI
MS.gene014206	MS.gene006957	PPI
MS.gene072269	MS.gene014206	PPI
MS.gene014206	MS.gene54792	PPI
MS.gene004953	MS.gene014206	PPI
MS.gene014206	MS.gene67362	PPI
MS.gene025056	MS.gene014206	PPI
MS.gene062190	MS.gene014206	PPI
MS.gene025449	MS.gene014206	PPI
MS.gene014206	MS.gene067014	PPI
MS.gene014206	MS.gene36342	PPI
MS.gene49432	MS.gene014206	PPI
MS.gene014206	MS.gene048817	PPI
MS.gene014206	MS.gene069812	PPI
MS.gene014206	MS.gene79774	PPI
MS.gene85141	MS.gene014206	PPI
MS.gene61127	MS.gene014206	PPI
MS.gene006959	MS.gene014206	PPI
MS.gene014206	MS.gene85141	PPI
MS.gene32446	MS.gene014206	PPI
MS.gene72127	MS.gene014206	PPI
MS.gene31709	MS.gene014206	PPI
MS.gene71137	MS.gene014206	PPI
MS.gene34830	MS.gene014206	PPI
MS.gene019314	MS.gene014206	PPI
MS.gene014206	MS.gene89899	PPI
MS.gene006960	MS.gene014206	PPI
MS.gene72129	MS.gene014206	PPI
MS.gene021393	MS.gene014206	PPI
MS.gene014206	MS.gene004088	PPI
MS.gene014206	MS.gene052743	PPI
MS.gene014206	MS.gene059531	PPI
MS.gene34032	MS.gene014206	PPI
MS.gene014206	MS.gene21620	PPI
MS.gene07481	MS.gene014206	PPI
MS.gene014206	MS.gene33486	PPI
MS.gene014206	MS.gene059380	PPI
MS.gene32445	MS.gene014206	PPI
MS.gene022891	MS.gene014206	PPI
MS.gene072777	MS.gene014206	PPI
MS.gene014206	MS.gene037437	PPI
MS.gene024637	MS.gene014206	PPI
MS.gene014206	MS.gene069860	PPI
MS.gene021061	MS.gene014206	PPI
MS.gene014206	MS.gene022891	PPI
MS.gene83316	MS.gene014206	PPI
MS.gene014206	MS.gene019314	PPI
MS.gene014206	MS.gene042849	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014206.t1	MTR_3g118010	96.099	282	4	2	1	278	1	279	0.0	536
MS.gene014206.t1	MTR_7g101190	78.746	287	46	4	1	275	1	284	1.06e-158	443
MS.gene014206.t1	MTR_2g094270	73.427	286	65	2	1	275	1	286	1.38e-152	427
MS.gene014206.t1	MTR_4g059390	74.823	282	58	3	4	275	6	284	6.31e-152	426
MS.gene014206.t1	MTR_2g094270	73.600	250	59	1	1	243	1	250	3.34e-133	378
MS.gene014206.t1	MTR_4g415300	69.141	256	75	1	17	268	33	288	2.51e-130	371
MS.gene014206.t1	MTR_8g098375	68.401	269	80	2	4	267	16	284	4.52e-130	370
MS.gene014206.t1	MTR_3g070210	70.881	261	71	2	13	268	28	288	6.82e-130	370
MS.gene014206.t1	MTR_5g082070	70.817	257	70	2	17	268	32	288	1.04e-127	365
MS.gene014206.t1	MTR_5g010150	67.037	270	84	2	4	268	16	285	1.93e-126	361
MS.gene014206.t1	MTR_3g070210	73.191	235	62	1	13	246	28	262	1.19e-120	346
MS.gene014206.t1	MTR_1g095070	81.818	198	32	1	84	277	23	220	9.97e-113	324
MS.gene014206.t1	MTR_4g059390	72.500	200	46	2	4	197	6	202	3.21e-100	291
MS.gene014206.t1	MTR_1g095070	83.750	160	26	0	84	243	23	182	6.85e-92	270
MS.gene014206.t1	MTR_3g070210	71.505	186	52	1	13	197	28	213	1.27e-90	268
MS.gene014206.t1	MTR_8g098375	64.115	209	72	2	9	214	21	229	2.20e-90	268
MS.gene014206.t1	MTR_4g415300	69.061	181	56	0	17	197	33	213	2.61e-89	265
MS.gene014206.t1	MTR_5g010150	68.280	186	58	1	13	197	25	210	1.79e-86	257
MS.gene014206.t1	MTR_2g101370	41.395	215	112	5	40	251	20	223	3.25e-38	135
MS.gene014206.t1	MTR_8g013680	41.284	218	108	6	40	251	19	222	2.12e-36	130
MS.gene014206.t1	MTR_4g063090	37.768	233	126	6	45	269	26	247	4.77e-34	124
MS.gene014206.t1	MTR_5g012810	40.000	215	114	5	40	251	19	221	4.82e-32	119
MS.gene014206.t1	MTR_1g077540	38.095	231	121	8	40	261	21	238	5.50e-32	119
MS.gene014206.t1	MTR_1g115405	38.356	219	121	5	40	255	21	228	9.49e-32	118
MS.gene014206.t1	MTR_7g023350	36.607	224	128	5	40	260	21	233	2.20e-31	117
MS.gene014206.t1	MTR_1g006490	36.406	217	116	7	40	251	19	218	4.87e-30	113
MS.gene014206.t1	MTR_1g006590	36.842	228	118	9	40	260	19	227	1.25e-29	112
MS.gene014206.t1	MTR_7g103030	35.814	215	123	5	40	251	21	223	1.64e-29	112
MS.gene014206.t1	MTR_6g069040	37.900	219	122	5	40	255	21	228	2.43e-29	112
MS.gene014206.t1	MTR_8g087710	30.415	217	136	5	38	254	37	238	6.46e-21	90.1
MS.gene014206.t1	MTR_2g104940	32.227	211	127	6	44	254	84	278	1.32e-20	89.7
MS.gene014206.t1	MTR_2g017660	30.233	215	135	5	40	254	39	238	6.19e-20	87.4
MS.gene014206.t1	MTR_4g075570	28.205	234	145	8	39	257	50	275	5.43e-19	85.5
MS.gene014206.t1	MTR_4g006730	28.315	279	159	10	7	273	55	304	5.94e-19	85.1
MS.gene014206.t1	MTR_5g063930	28.636	220	142	5	37	256	35	239	2.31e-18	82.8
MS.gene014206.t1	MTR_4g075590	32.086	187	111	6	87	260	81	264	3.73e-18	83.2
MS.gene014206.t1	MTR_5g079570	29.358	218	138	5	37	254	37	238	7.18e-18	81.6
MS.gene014206.t1	MTR_2g060360	31.250	224	127	7	40	254	19	224	8.02e-18	81.3
MS.gene014206.t1	MTR_3g008920	27.711	249	159	7	34	278	20	251	1.48e-17	80.5
MS.gene014206.t1	MTR_8g087720	29.817	218	138	5	37	254	35	237	1.54e-17	80.5
MS.gene014206.t1	MTR_4g084880	28.251	223	142	6	40	257	35	244	4.88e-17	79.3
MS.gene014206.t1	MTR_2g017590	29.493	217	138	5	38	254	34	235	7.34e-16	75.9
MS.gene014206.t1	MTR_7g010650	28.767	219	136	7	38	254	35	235	1.82e-15	74.7
MS.gene014206.t1	MTR_2g017620	29.032	217	139	5	38	254	22	223	2.42e-15	74.3
MS.gene014206.t1	MTR_2g017620	29.032	217	139	5	38	254	36	237	3.23e-15	73.9
MS.gene014206.t1	MTR_2g017610	29.907	214	125	7	40	248	38	231	5.92e-15	73.6
MS.gene014206.t1	MTR_2g017570	28.638	213	129	6	40	248	38	231	1.39e-14	72.4
MS.gene014206.t1	MTR_4g006630	27.897	233	140	8	53	273	1	217	4.11e-14	70.1
MS.gene014206.t1	MTR_4g006730	27.897	233	140	8	53	273	1	217	4.24e-14	70.1
MS.gene014206.t1	MTR_1g097840	28.505	214	137	6	37	250	71	268	1.10e-13	70.1
MS.gene014206.t1	MTR_1g070385	26.667	225	127	8	37	250	59	256	2.84e-13	70.1
MS.gene014206.t1	MTR_2g017620	30.357	168	105	4	87	254	1	156	8.17e-12	63.2
MS.gene014206.t1	MTR_4g006730	30.220	182	105	7	7	187	55	215	5.63e-11	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014206.t1	AT4G35100	89.007	282	25	2	1	278	1	280	0.0	504
MS.gene014206.t1	AT4G35100	89.007	282	25	2	1	278	1	280	0.0	504
MS.gene014206.t1	AT2G16850	89.716	282	21	2	1	278	1	278	0.0	503
MS.gene014206.t1	AT3G54820	74.476	286	61	3	1	276	1	284	4.90e-150	421
MS.gene014206.t1	AT5G60660	74.296	284	62	3	1	274	1	283	1.38e-149	420
MS.gene014206.t1	AT3G53420	77.239	268	51	2	17	274	16	283	1.93e-148	417
MS.gene014206.t1	AT3G53420	77.239	268	51	2	17	274	16	283	1.93e-148	417
MS.gene014206.t1	AT2G37170	72.917	288	64	4	1	274	37	324	4.31e-148	418
MS.gene014206.t1	AT2G37170	73.868	287	56	5	1	274	1	281	1.09e-147	415
MS.gene014206.t1	AT2G39010	73.077	286	63	4	1	275	1	283	3.86e-147	414
MS.gene014206.t1	AT2G37180	74.216	287	55	5	1	274	1	281	4.53e-146	411
MS.gene014206.t1	AT4G00430	73.828	256	63	1	17	268	30	285	1.60e-135	384
MS.gene014206.t1	AT2G45960	71.875	256	68	1	17	268	29	284	1.29e-131	374
MS.gene014206.t1	AT1G01620	73.438	256	64	1	17	268	29	284	3.60e-128	365
MS.gene014206.t1	AT4G23400	69.888	269	77	1	4	268	17	285	2.47e-125	358
MS.gene014206.t1	AT3G61430	71.094	256	70	1	17	268	29	284	1.96e-123	353
MS.gene014206.t1	AT3G61430	71.094	256	70	1	17	268	29	284	1.96e-123	353
MS.gene014206.t1	AT2G45960	75.330	227	56	0	17	243	29	255	5.79e-123	352
MS.gene014206.t1	AT2G45960	75.330	227	56	0	17	243	29	255	1.17e-121	350
MS.gene014206.t1	AT1G01620	74.528	212	50	1	61	268	1	212	4.44e-111	320
MS.gene014206.t1	AT2G39010	71.569	204	48	3	1	197	1	201	3.80e-98	287
MS.gene014206.t1	AT4G00430	75.135	185	46	0	17	201	30	214	2.43e-96	282
MS.gene014206.t1	AT3G16240	42.791	215	109	5	40	251	19	222	2.25e-37	133
MS.gene014206.t1	AT5G47450	39.648	227	123	6	37	260	16	231	6.02e-35	126
MS.gene014206.t1	AT4G01470	37.900	219	122	5	40	255	21	228	3.27e-34	124
MS.gene014206.t1	AT3G26520	38.889	216	116	6	40	251	22	225	5.82e-33	121
MS.gene014206.t1	AT4G17340	38.017	242	133	7	40	278	19	246	5.40e-31	116
MS.gene014206.t1	AT2G36830	36.744	215	122	5	40	251	21	224	2.10e-30	114
MS.gene014206.t1	AT1G73190	36.607	224	123	5	40	255	24	236	3.11e-30	114
MS.gene014206.t1	AT1G17810	37.872	235	122	6	40	261	24	247	8.49e-30	113
MS.gene014206.t1	AT2G25810	36.522	230	130	7	40	267	19	234	4.36e-29	111
MS.gene014206.t1	AT3G47440	32.432	222	129	8	40	255	23	229	1.00e-22	94.4
MS.gene014206.t1	AT4G19030	26.848	257	169	5	1	254	21	261	3.15e-17	80.1
MS.gene014206.t1	AT1G80760	28.899	218	139	5	37	254	77	278	7.75e-17	79.3
MS.gene014206.t1	AT1G31885	27.027	222	146	5	37	254	40	249	7.78e-16	76.6
MS.gene014206.t1	AT5G37820	29.907	214	131	6	40	251	43	239	1.52e-15	75.1
MS.gene014206.t1	AT5G37820	29.907	214	131	6	40	251	79	275	1.65e-15	75.5
MS.gene014206.t1	AT4G10380	29.439	214	135	5	37	250	75	272	2.69e-15	74.7
MS.gene014206.t1	AT5G37810	27.273	253	163	7	2	251	5	239	2.33e-14	72.0
MS.gene014206.t1	AT4G18910	26.364	220	148	4	38	254	50	258	4.69e-14	71.2
MS.gene014206.t1	AT1G31885	27.957	186	121	4	73	254	19	195	6.13e-12	64.7
MS.gene014206.t1	AT2G34390	28.571	217	138	7	38	250	46	249	9.69e-12	64.3
MS.gene014206.t1	AT3G06100	29.185	233	140	8	40	267	46	258	2.99e-11	62.8
MS.gene014206.t1	AT2G34390	28.440	218	139	7	37	250	3	207	4.51e-11	62.0

Find 101 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTGACGGTGTTGGCCTTCT+AGG	0.206372	3.4:+100084681	MS.gene014206:CDS
GAGTCCCGTGCGCTTCTCTT+AGG	0.269420	3.4:-100085029	None:intergenic
TCAAGGAGGGGTGCTGGTGG+TGG	0.295321	3.4:-100084537	None:intergenic
TATGCATGCAGTGGATCTTC+TGG	0.301366	3.4:+100085292	MS.gene014206:intron
TCTTCAACAATGCCAAAGTT+TGG	0.312604	3.4:+100085171	MS.gene014206:CDS
GGACTCACATGTCCCTGTTT+TGG	0.328630	3.4:+100085046	MS.gene014206:CDS
AATTAATTAACTAATTTAGT+TGG	0.361626	3.4:-100085403	None:intergenic
CTAGGCATAGCATGGTCCTT+TGG	0.364281	3.4:+100084699	MS.gene014206:CDS
ATGGGCAAAGGAGCCAAAAC+AGG	0.383343	3.4:-100085059	None:intergenic
CCGCCGCCGAGGTGGTTGTA+AGG	0.390135	3.4:-100084906	None:intergenic
CTTCAACAATGCCAAAGTTT+GGG	0.399277	3.4:+100085172	MS.gene014206:CDS
GGCAGAGCCTTTGCTGTAAC+CGG	0.402530	3.4:-100084946	None:intergenic
CCGCTCTAAGAATGTATTGA+TGG	0.413506	3.4:-100085341	None:intergenic
CAAGGAGGGGTGCTGGTGGT+GGG	0.413906	3.4:-100084536	None:intergenic
GTCCTTGTCTACTGCACCGC+TGG	0.418520	3.4:+100084735	MS.gene014206:CDS
GCCCTCGGTGCTGAAATTAT+CGG	0.421803	3.4:+100084966	MS.gene014206:CDS
AGGACCATGCTATGCCTAGA+AGG	0.427239	3.4:-100084695	None:intergenic
GTTGGTGGGGTTGCTTCTAA+AGG	0.429287	3.4:-100085385	None:intergenic
AATTAACTAATTTAGTTGGT+GGG	0.430160	3.4:-100085399	None:intergenic
TACTGCACCGCTGGTATCTC+CGG	0.434993	3.4:+100084744	MS.gene014206:CDS
AGTCCCGTGCGCTTCTCTTA+GGG	0.437868	3.4:-100085028	None:intergenic
GCTCCTAGCAGGGTTGATTC+CGG	0.438753	3.4:-100085132	None:intergenic
TTGATTCCGGTACCGGTGAT+TGG	0.445472	3.4:-100085119	None:intergenic
ATCAACCCTGCTAGGAGCTT+CGG	0.445533	3.4:+100085137	MS.gene014206:CDS
TACATGGTAGCACAGTGTTT+AGG	0.447822	3.4:+100084840	MS.gene014206:CDS
GCGGCGGCTATCAAGGCGTT+GGG	0.451615	3.4:+100085360	MS.gene014206:CDS
TCTAAAGAAGTGAGTGAAGA+AGG	0.451723	3.4:+100084477	MS.gene014206:CDS
AGCGGCGGCTATCAAGGCGT+TGG	0.453049	3.4:+100085359	MS.gene014206:CDS
TCTTAGAGCGGCGGCTATCA+AGG	0.465345	3.4:+100085353	MS.gene014206:CDS
AGCAGGGTTGATTCCGGTAC+CGG	0.467989	3.4:-100085126	None:intergenic
ATTAACCCTGCTGTCACATT+CGG	0.470489	3.4:+100084774	MS.gene014206:CDS
GCGAAGTCAAGGAGGGGTGC+TGG	0.483574	3.4:-100084543	None:intergenic
TGCTATCTGTGGCGTTGGGT+TGG	0.487734	3.4:+100084863	MS.gene014206:CDS
GATCTTCTGGGTTGGACCGT+TGG	0.489786	3.4:+100085305	MS.gene014206:CDS
GTCACTGTCGCCACCGTCAT+TGG	0.492002	3.4:+100084636	MS.gene014206:CDS
CTGTGGCGTTGGGTTGGTGA+AGG	0.493175	3.4:+100084869	MS.gene014206:CDS
TAATTAACTAATTTAGTTGG+TGG	0.496888	3.4:-100085400	None:intergenic
GGCATAGCATGGTCCTTTGG+TGG	0.497936	3.4:+100084702	MS.gene014206:CDS
GCAGCACCGAAGCTCCTAGC+AGG	0.500446	3.4:-100085143	None:intergenic
TACAGCAAAGGCTCTGCCCT+CGG	0.501785	3.4:+100084951	MS.gene014206:CDS
TTAGGTGCTATCTGTGGCGT+TGG	0.502610	3.4:+100084858	MS.gene014206:CDS
CGCCGCCGAGGTGGTTGTAA+GGG	0.503836	3.4:-100084905	None:intergenic
ACACTTTACGAGCCAAGAAA+AGG	0.506621	3.4:-100084797	None:intergenic
CGACAGTGACGTAGAGAAAG+AGG	0.507110	3.4:-100084623	None:intergenic
ATTAACTAATTTAGTTGGTG+GGG	0.507877	3.4:-100085398	None:intergenic
ACTATACCAATCACCGGTAC+CGG	0.509500	3.4:+100085113	MS.gene014206:CDS
TACATGGTCATCCCAAACTT+TGG	0.513712	3.4:-100085183	None:intergenic
CATAAGAGCGGTGTTATACA+TGG	0.519758	3.4:+100084824	MS.gene014206:CDS
AAAAGGCCGAATGTGACAGC+AGG	0.520051	3.4:-100084780	None:intergenic
ATGCATGCAGTGGATCTTCT+GGG	0.527197	3.4:+100085293	MS.gene014206:intron
CTGGGTTGGACCGTTGGCAG+CGG	0.534536	3.4:+100085311	MS.gene014206:CDS
CCTTACAACCACCTCGGCGG+CGG	0.549715	3.4:+100084906	MS.gene014206:CDS
GGTGCTAACTCTGTAGCTTC+CGG	0.550093	3.4:+100084927	MS.gene014206:CDS
GCTTAATCTCAGCGAAGTCA+AGG	0.551663	3.4:-100084554	None:intergenic
TAGGTGCTATCTGTGGCGTT+GGG	0.554325	3.4:+100084859	MS.gene014206:CDS
TAGTCCTTACCGTGAGGATG+TGG	0.555862	3.4:-100084507	None:intergenic
TCCGATAATTTCAGCACCGA+GGG	0.562447	3.4:-100084967	None:intergenic
AAGTCAAGGAGGGGTGCTGG+TGG	0.564356	3.4:-100084540	None:intergenic
GTAGCTTCCGGTTACAGCAA+AGG	0.567822	3.4:+100084939	MS.gene014206:CDS
TGGTATGCAGCCGCTGCCAA+CGG	0.572803	3.4:-100085321	None:intergenic
ACAGCAAAACCAATGGGCAA+AGG	0.573216	3.4:-100085071	None:intergenic
AGCAGGGTTAATGTGGCCAC+CGG	0.573223	3.4:-100084763	None:intergenic
TTGGCCTTCTAGGCATAGCA+TGG	0.579145	3.4:+100084691	MS.gene014206:CDS
CCTACCACATCCTCACGGTA+AGG	0.579509	3.4:+100084503	MS.gene014206:CDS
CTAAAGAAGTGAGTGAAGAA+GGG	0.592831	3.4:+100084478	MS.gene014206:CDS
TACCAGCGGTGCAGTAGACA+AGG	0.595575	3.4:-100084737	None:intergenic
GACAAAGATCATGCCACCAA+AGG	0.596121	3.4:-100084715	None:intergenic
ACTTCGCTGAGATTAAGCTC+TGG	0.596756	3.4:+100084559	MS.gene014206:CDS
ATGTGACAGCAGGGTTAATG+TGG	0.601114	3.4:-100084770	None:intergenic
CGGTGATTGGTATAGTTGCA+AGG	0.601911	3.4:-100085106	None:intergenic
CAGAGTTAGCACCGCCGCCG+AGG	0.603070	3.4:-100084917	None:intergenic
CAGCACCGAAGCTCCTAGCA+GGG	0.610916	3.4:-100085142	None:intergenic
GTACCGGAATCAACCCTGCT+AGG	0.610944	3.4:+100085129	MS.gene014206:CDS
AATCTCAGCGAAGTCAAGGA+GGG	0.616287	3.4:-100084550	None:intergenic
AGAAGGCCAACACCGTCACA+TGG	0.616986	3.4:-100084678	None:intergenic
ATGCAGTGGATCTTCTGGGT+TGG	0.623221	3.4:+100085297	MS.gene014206:intron
TGCACCGCTGGTATCTCCGG+TGG	0.624717	3.4:+100084747	MS.gene014206:CDS
ATCTCAGCGAAGTCAAGGAG+GGG	0.630738	3.4:-100084549	None:intergenic
CCTTACCGTGAGGATGTGGT+AGG	0.631606	3.4:-100084503	None:intergenic
TGACCCTAAGAGAAGCGCAC+GGG	0.634134	3.4:+100085025	MS.gene014206:CDS
AAACAAACTGGTCCATGTGA+CGG	0.634416	3.4:+100084666	MS.gene014206:CDS
CTTGCAACTATACCAATCAC+CGG	0.635837	3.4:+100085107	MS.gene014206:CDS
TGGGCAAAGGAGCCAAAACA+GGG	0.637363	3.4:-100085058	None:intergenic
AGTTAGCACCGCCGCCGAGG+TGG	0.643604	3.4:-100084914	None:intergenic
CAGTGACGTAGAGAAAGAGG+AGG	0.644359	3.4:-100084620	None:intergenic
CAGTGTTTAGGTGCTATCTG+TGG	0.653498	3.4:+100084852	MS.gene014206:CDS
AAAGGCCGAATGTGACAGCA+GGG	0.654056	3.4:-100084779	None:intergenic
CACCCTTACAACCACCTCGG+CGG	0.654519	3.4:+100084903	MS.gene014206:CDS
TAATCTCAGCGAAGTCAAGG+AGG	0.655437	3.4:-100084551	None:intergenic
ACTGGTCCATGTGACGGTGT+TGG	0.657929	3.4:+100084672	MS.gene014206:CDS
TTCCGATAATTTCAGCACCG+AGG	0.665140	3.4:-100084968	None:intergenic
TCAATACATTCTTAGAGCGG+CGG	0.665343	3.4:+100085344	MS.gene014206:CDS
CTGACCCTAAGAGAAGCGCA+CGG	0.668284	3.4:+100085024	MS.gene014206:CDS
CCATCAATACATTCTTAGAG+CGG	0.687313	3.4:+100085341	MS.gene014206:CDS
TAAAGTGTCTCTCATAAGAG+CGG	0.688091	3.4:+100084812	MS.gene014206:CDS
AAACACCCTTACAACCACCT+CGG	0.690282	3.4:+100084900	MS.gene014206:CDS
AGTGACGTAGAGAAAGAGGA+GGG	0.690484	3.4:-100084619	None:intergenic
GGGCACCTACCACATCCTCA+CGG	0.692416	3.4:+100084498	MS.gene014206:CDS
GACGTAGAGAAAGAGGAGGG+TGG	0.694343	3.4:-100084616	None:intergenic
GTGGCCACCGGAGATACCAG+CGG	0.716451	3.4:-100084751	None:intergenic
TCTACGTAGTCCTTACCGTG+AGG	0.728979	3.4:-100084513	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAGATATAATTAACATACA+TGG	-	chr3.4:100085202-100085221	None:intergenic	15.0%
!	ATAAAAACAGCAAAACCAAT+GGG	-	chr3.4:100085080-100085099	None:intergenic	25.0%
	ATTGGTCACAAAAAACAAAC+TGG	+	chr3.4:100084654-100084673	MS.gene014206:CDS	30.0%
	CATAAAAACAGCAAAACCAA+TGG	-	chr3.4:100085081-100085100	None:intergenic	30.0%
	TATACTATATATGCATGCAG+TGG	+	chr3.4:100085283-100085302	MS.gene014206:intron	30.0%
!	AATTATCGGAACTTTTGTGT+TGG	+	chr3.4:100084980-100084999	MS.gene014206:CDS	30.0%
!!!	CATTGGTTTTGCTGTTTTTA+TGG	+	chr3.4:100085079-100085098	MS.gene014206:CDS	30.0%
!!!	TTGTTTTTTGTGACCAATGA+CGG	-	chr3.4:100084652-100084671	None:intergenic	30.0%
	CCATCAATACATTCTTAGAG+CGG	+	chr3.4:100085341-100085360	MS.gene014206:CDS	35.0%
	CTAAAGAAGTGAGTGAAGAA+GGG	+	chr3.4:100084478-100084497	MS.gene014206:CDS	35.0%
	CTTCAACAATGCCAAAGTTT+GGG	+	chr3.4:100085172-100085191	MS.gene014206:CDS	35.0%
	TCTAAAGAAGTGAGTGAAGA+AGG	+	chr3.4:100084477-100084496	MS.gene014206:CDS	35.0%
	TCTTCAACAATGCCAAAGTT+TGG	+	chr3.4:100085171-100085190	MS.gene014206:CDS	35.0%
!	TAAAGTGTCTCTCATAAGAG+CGG	+	chr3.4:100084812-100084831	MS.gene014206:CDS	35.0%
	AAACAAACTGGTCCATGTGA+CGG	+	chr3.4:100084666-100084685	MS.gene014206:CDS	40.0%
	ACACTTTACGAGCCAAGAAA+AGG	-	chr3.4:100084800-100084819	None:intergenic	40.0%
	ATTAACCCTGCTGTCACATT+CGG	+	chr3.4:100084774-100084793	MS.gene014206:CDS	40.0%
	CTTGCAACTATACCAATCAC+CGG	+	chr3.4:100085107-100085126	MS.gene014206:CDS	40.0%
	TACATGGTAGCACAGTGTTT+AGG	+	chr3.4:100084840-100084859	MS.gene014206:CDS	40.0%
	TACATGGTCATCCCAAACTT+TGG	-	chr3.4:100085186-100085205	None:intergenic	40.0%
	TCAATACATTCTTAGAGCGG+CGG	+	chr3.4:100085344-100085363	MS.gene014206:CDS	40.0%
!!	CATAAGAGCGGTGTTATACA+TGG	+	chr3.4:100084824-100084843	MS.gene014206:CDS	40.0%
!!	CCGCTCTAAGAATGTATTGA+TGG	-	chr3.4:100085344-100085363	None:intergenic	40.0%
!!!	TTTTTTGTGACCAATGACGG+TGG	-	chr3.4:100084649-100084668	None:intergenic	40.0%
	AAACACCCTTACAACCACCT+CGG	+	chr3.4:100084900-100084919	MS.gene014206:CDS	45.0%
	AATCTCAGCGAAGTCAAGGA+GGG	-	chr3.4:100084553-100084572	None:intergenic	45.0%
	ACAGCAAAACCAATGGGCAA+AGG	-	chr3.4:100085074-100085093	None:intergenic	45.0%
	ACTATACCAATCACCGGTAC+CGG	+	chr3.4:100085113-100085132	MS.gene014206:CDS	45.0%
	ACTTCGCTGAGATTAAGCTC+TGG	+	chr3.4:100084559-100084578	MS.gene014206:CDS	45.0%
	AGTGACGTAGAGAAAGAGGA+GGG	-	chr3.4:100084622-100084641	None:intergenic	45.0%
	ATGCATGCAGTGGATCTTCT+GGG	+	chr3.4:100085293-100085312	MS.gene014206:intron	45.0%
	ATGTGACAGCAGGGTTAATG+TGG	-	chr3.4:100084773-100084792	None:intergenic	45.0%
	CGGTGATTGGTATAGTTGCA+AGG	-	chr3.4:100085109-100085128	None:intergenic	45.0%
	GACAAAGATCATGCCACCAA+AGG	-	chr3.4:100084718-100084737	None:intergenic	45.0%
	GCTTAATCTCAGCGAAGTCA+AGG	-	chr3.4:100084557-100084576	None:intergenic	45.0%
	TAATCTCAGCGAAGTCAAGG+AGG	-	chr3.4:100084554-100084573	None:intergenic	45.0%
	TATGCATGCAGTGGATCTTC+TGG	+	chr3.4:100085292-100085311	MS.gene014206:intron	45.0%
	TTCCGATAATTTCAGCACCG+AGG	-	chr3.4:100084971-100084990	None:intergenic	45.0%
!	CAGTGTTTAGGTGCTATCTG+TGG	+	chr3.4:100084852-100084871	MS.gene014206:CDS	45.0%
!	TCCGATAATTTCAGCACCGA+GGG	-	chr3.4:100084970-100084989	None:intergenic	45.0%
!	TGTCACATTCGGCCTTTTCT+TGG	+	chr3.4:100084785-100084804	MS.gene014206:CDS	45.0%
	AAAAGGCCGAATGTGACAGC+AGG	-	chr3.4:100084783-100084802	None:intergenic	50.0%
	AAAGGCCGAATGTGACAGCA+GGG	-	chr3.4:100084782-100084801	None:intergenic	50.0%
	AGGACCATGCTATGCCTAGA+AGG	-	chr3.4:100084698-100084717	None:intergenic	50.0%
	ATCAACCCTGCTAGGAGCTT+CGG	+	chr3.4:100085137-100085156	MS.gene014206:CDS	50.0%
	ATCTCAGCGAAGTCAAGGAG+GGG	-	chr3.4:100084552-100084571	None:intergenic	50.0%
	ATGCAGTGGATCTTCTGGGT+TGG	+	chr3.4:100085297-100085316	MS.gene014206:intron	50.0%
	ATGGGCAAAGGAGCCAAAAC+AGG	-	chr3.4:100085062-100085081	None:intergenic	50.0%
	CAGTGACGTAGAGAAAGAGG+AGG	-	chr3.4:100084623-100084642	None:intergenic	50.0%
	CGACAGTGACGTAGAGAAAG+AGG	-	chr3.4:100084626-100084645	None:intergenic	50.0%
	CTAGGCATAGCATGGTCCTT+TGG	+	chr3.4:100084699-100084718	MS.gene014206:CDS	50.0%
	GGTGCTAACTCTGTAGCTTC+CGG	+	chr3.4:100084927-100084946	MS.gene014206:CDS	50.0%
	GTAGCTTCCGGTTACAGCAA+AGG	+	chr3.4:100084939-100084958	MS.gene014206:CDS	50.0%
	TAGTCCTTACCGTGAGGATG+TGG	-	chr3.4:100084510-100084529	None:intergenic	50.0%
	TCTACGTAGTCCTTACCGTG+AGG	-	chr3.4:100084516-100084535	None:intergenic	50.0%
	TGGGCAAAGGAGCCAAAACA+GGG	-	chr3.4:100085061-100085080	None:intergenic	50.0%
!	GGACTCACATGTCCCTGTTT+TGG	+	chr3.4:100085046-100085065	MS.gene014206:CDS	50.0%
!	GTTGGTGGGGTTGCTTCTAA+AGG	-	chr3.4:100085388-100085407	None:intergenic	50.0%
!	TAGGTGCTATCTGTGGCGTT+GGG	+	chr3.4:100084859-100084878	MS.gene014206:CDS	50.0%
!	TTAGGTGCTATCTGTGGCGT+TGG	+	chr3.4:100084858-100084877	MS.gene014206:CDS	50.0%
!	TTGGCCTTCTAGGCATAGCA+TGG	+	chr3.4:100084691-100084710	MS.gene014206:CDS	50.0%
!!	GCCCTCGGTGCTGAAATTAT+CGG	+	chr3.4:100084966-100084985	MS.gene014206:CDS	50.0%
!!	TTGATTCCGGTACCGGTGAT+TGG	-	chr3.4:100085122-100085141	None:intergenic	50.0%
!!!	GTTTTGGCTCCTTTGCCCAT+TGG	+	chr3.4:100085062-100085081	MS.gene014206:CDS	50.0%
	ACTGGTCCATGTGACGGTGT+TGG	+	chr3.4:100084672-100084691	MS.gene014206:CDS	55.0%
	AGAAGGCCAACACCGTCACA+TGG	-	chr3.4:100084681-100084700	None:intergenic	55.0%
	AGCAGGGTTAATGTGGCCAC+CGG	-	chr3.4:100084766-100084785	None:intergenic	55.0%
	AGTCCCGTGCGCTTCTCTTA+GGG	-	chr3.4:100085031-100085050	None:intergenic	55.0%
	CCTACCACATCCTCACGGTA+AGG	+	chr3.4:100084503-100084522	MS.gene014206:CDS	55.0%
	CCTTACCGTGAGGATGTGGT+AGG	-	chr3.4:100084506-100084525	None:intergenic	55.0%
	GACGTAGAGAAAGAGGAGGG+TGG	-	chr3.4:100084619-100084638	None:intergenic	55.0%
	GGCATAGCATGGTCCTTTGG+TGG	+	chr3.4:100084702-100084721	MS.gene014206:CDS	55.0%
	GTACCGGAATCAACCCTGCT+AGG	+	chr3.4:100085129-100085148	MS.gene014206:CDS	55.0%
	TACAGCAAAGGCTCTGCCCT+CGG	+	chr3.4:100084951-100084970	MS.gene014206:CDS	55.0%
	TACCAGCGGTGCAGTAGACA+AGG	-	chr3.4:100084740-100084759	None:intergenic	55.0%
	TACTGCACCGCTGGTATCTC+CGG	+	chr3.4:100084744-100084763	MS.gene014206:CDS	55.0%
	TCTTAGAGCGGCGGCTATCA+AGG	+	chr3.4:100085353-100085372	MS.gene014206:CDS	55.0%
!	AGCAGGGTTGATTCCGGTAC+CGG	-	chr3.4:100085129-100085148	None:intergenic	55.0%
!	CTGACCCTAAGAGAAGCGCA+CGG	+	chr3.4:100085024-100085043	MS.gene014206:CDS	55.0%
!	GATCTTCTGGGTTGGACCGT+TGG	+	chr3.4:100085305-100085324	MS.gene014206:CDS	55.0%
!	GCTCCTAGCAGGGTTGATTC+CGG	-	chr3.4:100085135-100085154	None:intergenic	55.0%
!	GGCAGAGCCTTTGCTGTAAC+CGG	-	chr3.4:100084949-100084968	None:intergenic	55.0%
!	TGACCCTAAGAGAAGCGCAC+GGG	+	chr3.4:100085025-100085044	MS.gene014206:CDS	55.0%
!	TGCTATCTGTGGCGTTGGGT+TGG	+	chr3.4:100084863-100084882	MS.gene014206:CDS	55.0%
!!	TGTGACGGTGTTGGCCTTCT+AGG	+	chr3.4:100084681-100084700	MS.gene014206:CDS	55.0%
	CACCCTTACAACCACCTCGG+CGG	+	chr3.4:100084903-100084922	MS.gene014206:CDS	60.0%
	GAGTCCCGTGCGCTTCTCTT+AGG	-	chr3.4:100085032-100085051	None:intergenic	60.0%
	GGGCACCTACCACATCCTCA+CGG	+	chr3.4:100084498-100084517	MS.gene014206:CDS	60.0%
	GTCACTGTCGCCACCGTCAT+TGG	+	chr3.4:100084636-100084655	MS.gene014206:CDS	60.0%
	GTCCTTGTCTACTGCACCGC+TGG	+	chr3.4:100084735-100084754	MS.gene014206:CDS	60.0%
	TGGTATGCAGCCGCTGCCAA+CGG	-	chr3.4:100085324-100085343	None:intergenic	60.0%
!	CAGCACCGAAGCTCCTAGCA+GGG	-	chr3.4:100085145-100085164	None:intergenic	60.0%
!!	AAGTCAAGGAGGGGTGCTGG+TGG	-	chr3.4:100084543-100084562	None:intergenic	60.0%
!!	CTGTGGCGTTGGGTTGGTGA+AGG	+	chr3.4:100084869-100084888	MS.gene014206:CDS	60.0%
	AGCGGCGGCTATCAAGGCGT+TGG	+	chr3.4:100085359-100085378	MS.gene014206:CDS	65.0%
	CCTTACAACCACCTCGGCGG+CGG	+	chr3.4:100084906-100084925	MS.gene014206:CDS	65.0%
	CGCCGCCGAGGTGGTTGTAA+GGG	-	chr3.4:100084908-100084927	None:intergenic	65.0%
	GTGGCCACCGGAGATACCAG+CGG	-	chr3.4:100084754-100084773	None:intergenic	65.0%
	TGCACCGCTGGTATCTCCGG+TGG	+	chr3.4:100084747-100084766	MS.gene014206:CDS	65.0%
!	CTGGGTTGGACCGTTGGCAG+CGG	+	chr3.4:100085311-100085330	MS.gene014206:CDS	65.0%
!	GCAGCACCGAAGCTCCTAGC+AGG	-	chr3.4:100085146-100085165	None:intergenic	65.0%
!	GCGAAGTCAAGGAGGGGTGC+TGG	-	chr3.4:100084546-100084565	None:intergenic	65.0%
!	GCGGCGGCTATCAAGGCGTT+GGG	+	chr3.4:100085360-100085379	MS.gene014206:CDS	65.0%
!!	CAAGGAGGGGTGCTGGTGGT+GGG	-	chr3.4:100084539-100084558	None:intergenic	65.0%
!!	TCAAGGAGGGGTGCTGGTGG+TGG	-	chr3.4:100084540-100084559	None:intergenic	65.0%
	AGTTAGCACCGCCGCCGAGG+TGG	-	chr3.4:100084917-100084936	None:intergenic	70.0%
	CAGAGTTAGCACCGCCGCCG+AGG	-	chr3.4:100084920-100084939	None:intergenic	70.0%
	CCGCCGCCGAGGTGGTTGTA+AGG	-	chr3.4:100084909-100084928	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	100084474	100085410	100084474	ID=MS.gene014206
chr3.4	mRNA	100084474	100085410	100084474	ID=MS.gene014206.t1;Parent=MS.gene014206
chr3.4	exon	100084474	100085202	100084474	ID=MS.gene014206.t1.exon1;Parent=MS.gene014206.t1
chr3.4	CDS	100084474	100085202	100084474	ID=cds.MS.gene014206.t1;Parent=MS.gene014206.t1
chr3.4	exon	100085303	100085410	100085303	ID=MS.gene014206.t1.exon2;Parent=MS.gene014206.t1
chr3.4	CDS	100085303	100085410	100085303	ID=cds.MS.gene014206.t1;Parent=MS.gene014206.t1

Gene Sequence

>MS.gene014206

ATGTCTAAAGAAGTGAGTGAAGAAGGGCACCTACCACATCCTCACGGTAAGGACTACGTAGACCCACCACCAGCACCCCTCCTTGACTTCGCTGAGATTAAGCTCTGGTCTTTCTACAGAGCTCTCATCGCTGAGTTCATAGCCACCCTCCTCTTTCTCTACGTCACTGTCGCCACCGTCATTGGTCACAAAAAACAAACTGGTCCATGTGACGGTGTTGGCCTTCTAGGCATAGCATGGTCCTTTGGTGGCATGATCTTTGTCCTTGTCTACTGCACCGCTGGTATCTCCGGTGGCCACATTAACCCTGCTGTCACATTCGGCCTTTTCTTGGCTCGTAAAGTGTCTCTCATAAGAGCGGTGTTATACATGGTAGCACAGTGTTTAGGTGCTATCTGTGGCGTTGGGTTGGTGAAGGCTTTCATGAAACACCCTTACAACCACCTCGGCGGCGGTGCTAACTCTGTAGCTTCCGGTTACAGCAAAGGCTCTGCCCTCGGTGCTGAAATTATCGGAACTTTTGTGTTGGTTTACACTGTTTTCTCTGCTACTGACCCTAAGAGAAGCGCACGGGACTCACATGTCCCTGTTTTGGCTCCTTTGCCCATTGGTTTTGCTGTTTTTATGGTTCACCTTGCAACTATACCAATCACCGGTACCGGAATCAACCCTGCTAGGAGCTTCGGTGCTGCTGTCATCTTCAACAATGCCAAAGTTTGGGATGACCATGTATGTTAATTATATCTTTCATTTCAATTTATATATAATTTTGACATGCAATTAATGCAAATTATTATAATTAACGTGTATATACTATATATGCATGCAGTGGATCTTCTGGGTTGGACCGTTGGCAGCGGCTGCATACCATCAATACATTCTTAGAGCGGCGGCTATCAAGGCGTTGGGCTCCTTTAGAAGCAACCCCACCAACTAA

Protein sequence

>MS.gene014206.t1

MSKEVSEEGHLPHPHGKDYVDPPPAPLLDFAEIKLWSFYRALIAEFIATLLFLYVTVATVIGHKKQTGPCDGVGLLGIAWSFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLIRAVLYMVAQCLGAICGVGLVKAFMKHPYNHLGGGANSVASGYSKGSALGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNNAKVWDDHWIFWVGPLAAAAYHQYILRAAAIKALGSFRSNPTN