AlfalfaGEDB | MS.gene72127

M. sativa cultivar XinJiangDaYe / MS.gene72127

KEGG: K09872
GO: GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0008150 GO:0015267 GO:0016020 GO:0022803 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0110165
PFAM: PF00230.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72127.t1	XP_013447045.1	99.3	287	2	0	1	287	1	287	4.10E-160	573.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72127.t1	Q7XSQ9	89.2	286	29	2	3	287	4	288	5.4e-145	515.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72127.t1	A0A072TUG6	99.3	287	2	0	1	287	1	287	2.9e-160	573.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049622	MS.gene72127	0.846552	2.02E-59	-1.69E-46
MS.gene04985	MS.gene72127	0.813752	2.18E-51	-1.69E-46
MS.gene049989	MS.gene72127	0.872522	2.98E-67	-1.69E-46
MS.gene050297	MS.gene72127	0.863983	1.69E-64	-1.69E-46
MS.gene050648	MS.gene72127	0.815847	7.50E-52	-1.69E-46
MS.gene051263	MS.gene72127	0.853419	2.42E-61	-1.69E-46
MS.gene051478	MS.gene72127	0.829299	5.56E-55	-1.69E-46
MS.gene051629	MS.gene72127	0.819183	1.33E-52	-1.69E-46
MS.gene051742	MS.gene72127	0.818307	2.10E-52	-1.69E-46
MS.gene051955	MS.gene72127	0.802668	5.03E-49	-1.69E-46
MS.gene052143	MS.gene72127	0.80846	3.06E-50	-1.69E-46
MS.gene052308	MS.gene72127	0.820779	5.73E-53	-1.69E-46
MS.gene052367	MS.gene72127	0.886399	3.54E-72	-1.69E-46
MS.gene053173	MS.gene72127	0.8473	1.26E-59	-1.69E-46
MS.gene053348	MS.gene72127	0.801436	9.01E-49	-1.69E-46
MS.gene053416	MS.gene72127	0.849852	2.48E-60	-1.69E-46
MS.gene053417	MS.gene72127	0.851009	1.17E-60	-1.69E-46
MS.gene05369	MS.gene72127	0.823748	1.17E-53	-1.69E-46
MS.gene054551	MS.gene72127	0.843573	1.29E-58	-1.69E-46
MS.gene054593	MS.gene72127	0.808309	3.30E-50	-1.69E-46
MS.gene054643	MS.gene72127	0.811982	5.33E-51	-1.69E-46
MS.gene055308	MS.gene72127	0.833203	6.10E-56	-1.69E-46
MS.gene055578	MS.gene72127	0.855289	6.97E-62	-1.69E-46
MS.gene055957	MS.gene72127	0.900042	1.09E-77	-1.69E-46
MS.gene056041	MS.gene72127	0.872287	3.57E-67	-1.69E-46
MS.gene056335	MS.gene72127	0.838195	3.32E-57	-1.69E-46
MS.gene05640	MS.gene72127	0.830507	2.82E-55	-1.69E-46
MS.gene056526	MS.gene72127	0.804086	2.56E-49	-1.69E-46
MS.gene057458	MS.gene72127	0.854342	1.31E-61	-1.69E-46
MS.gene057483	MS.gene72127	0.812579	3.95E-51	-1.69E-46
MS.gene058196	MS.gene72127	0.809466	1.87E-50	-1.69E-46
MS.gene058197	MS.gene72127	0.806318	8.72E-50	-1.69E-46
MS.gene058198	MS.gene72127	0.806733	7.13E-50	-1.69E-46
MS.gene058818	MS.gene72127	0.835834	1.33E-56	-1.69E-46
MS.gene058844	MS.gene72127	0.80353	3.34E-49	-1.69E-46
MS.gene058853	MS.gene72127	0.805024	1.63E-49	-1.69E-46
MS.gene059300	MS.gene72127	0.815277	1.00E-51	-1.69E-46
MS.gene060473	MS.gene72127	0.859312	4.51E-63	-1.69E-46
MS.gene060565	MS.gene72127	0.856044	4.20E-62	-1.69E-46
MS.gene06057	MS.gene72127	0.851783	7.09E-61	-1.69E-46
MS.gene06093	MS.gene72127	0.840629	7.75E-58	-1.69E-46
MS.gene061099	MS.gene72127	0.846035	2.80E-59	-1.69E-46
MS.gene061260	MS.gene72127	0.866675	2.40E-65	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene03094	MS.gene72127	PPI
MS.gene72127	MS.gene85141	PPI
MS.gene72127	MS.gene01625	PPI
MS.gene72127	MS.gene021394	PPI
MS.gene72127	MS.gene006957	PPI
MS.gene32446	MS.gene72127	PPI
MS.gene72127	MS.gene67362	PPI
MS.gene072777	MS.gene72127	PPI
MS.gene72127	MS.gene03090	PPI
MS.gene72127	MS.gene36342	PPI
MS.gene72127	MS.gene052743	PPI
MS.gene72127	MS.gene059531	PPI
MS.gene72127	MS.gene025449	PPI
MS.gene72127	MS.gene21620	PPI
MS.gene72127	MS.gene33486	PPI
MS.gene72127	MS.gene27853	PPI
MS.gene72127	MS.gene059380	PPI
MS.gene72127	MS.gene069812	PPI
MS.gene72127	MS.gene023622	PPI
MS.gene72127	MS.gene79774	PPI
MS.gene72127	MS.gene36973	PPI
MS.gene34032	MS.gene72127	PPI
MS.gene72127	MS.gene89899	PPI
MS.gene07481	MS.gene72127	PPI
MS.gene72127	MS.gene037437	PPI
MS.gene72127	MS.gene065973	PPI
MS.gene32445	MS.gene72127	PPI
MS.gene72127	MS.gene002643	PPI
MS.gene72127	MS.gene072777	PPI
MS.gene72127	MS.gene004324	PPI
MS.gene72127	MS.gene83316	PPI
MS.gene72127	MS.gene004088	PPI
MS.gene72127	MS.gene065972	PPI
MS.gene03093	MS.gene72127	PPI
MS.gene72127	MS.gene069860	PPI
MS.gene72127	MS.gene014206	PPI
MS.gene72127	MS.gene004322	PPI
MS.gene72127	MS.gene023621	PPI
MS.gene005974	MS.gene72127	PPI
MS.gene72127	MS.gene01627	PPI
MS.gene72127	MS.gene019314	PPI
MS.gene72127	MS.gene042849	PPI
MS.gene72127	MS.gene059379	PPI
MS.gene72127	MS.gene002639	PPI
MS.gene72127	MS.gene059378	PPI
MS.gene76526	MS.gene72127	PPI
MS.gene021061	MS.gene72127	PPI
MS.gene72127	MS.gene004321	PPI
MS.gene72127	MS.gene006961	PPI
MS.gene83316	MS.gene72127	PPI
MS.gene004953	MS.gene72127	PPI
MS.gene72127	MS.gene01624	PPI
MS.gene72127	MS.gene03121	PPI
MS.gene025449	MS.gene72127	PPI
MS.gene72127	MS.gene93201	PPI
MS.gene72127	MS.gene024637	PPI
MS.gene72127	MS.gene022891	PPI
MS.gene72127	MS.gene006959	PPI
MS.gene72127	MS.gene004953	PPI
MS.gene72127	MS.gene072269	PPI
MS.gene72127	MS.gene062190	PPI
MS.gene72127	MS.gene54792	PPI
MS.gene61127	MS.gene72127	PPI
MS.gene72127	MS.gene49432	PPI
MS.gene72127	MS.gene72129	PPI
MS.gene31709	MS.gene72127	PPI
MS.gene71137	MS.gene72127	PPI
MS.gene34830	MS.gene72127	PPI
MS.gene72127	MS.gene002640	PPI
MS.gene72127	MS.gene067014	PPI
MS.gene72127	MS.gene27852	PPI
MS.gene006960	MS.gene72127	PPI
MS.gene021393	MS.gene72127	PPI
MS.gene72127	MS.gene36789	PPI
MS.gene72127	MS.gene048817	PPI
MS.gene025056	MS.gene72127	PPI
MS.gene72127	MS.gene006958	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72127.t1	MTR_8g098375	99.303	287	2	0	1	287	1	287	0.0	581
MS.gene72127.t1	MTR_5g010150	92.657	286	21	0	1	286	1	286	0.0	550
MS.gene72127.t1	MTR_5g082070	86.364	286	37	1	3	286	4	289	0.0	510
MS.gene72127.t1	MTR_3g070210	87.719	285	33	1	3	285	4	288	0.0	510
MS.gene72127.t1	MTR_4g415300	82.986	288	45	2	2	286	3	289	3.41e-179	495
MS.gene72127.t1	MTR_3g070210	87.259	259	31	1	3	259	4	262	3.18e-164	457
MS.gene72127.t1	MTR_8g098375	92.140	229	16	1	1	227	1	229	1.44e-153	428
MS.gene72127.t1	MTR_5g010150	91.905	210	17	0	1	210	1	210	2.83e-144	404
MS.gene72127.t1	MTR_7g101190	71.218	271	68	3	16	280	6	272	5.25e-140	396
MS.gene72127.t1	MTR_3g118010	70.455	264	77	1	21	284	6	268	4.68e-135	383
MS.gene72127.t1	MTR_2g094270	64.261	291	84	3	1	284	1	278	6.20e-135	383
MS.gene72127.t1	MTR_4g059390	66.917	266	81	2	25	284	12	276	5.37e-131	373
MS.gene72127.t1	MTR_3g070210	86.190	210	27	1	3	210	4	213	9.62e-130	367
MS.gene72127.t1	MTR_4g415300	80.189	212	38	2	2	210	3	213	6.57e-124	353
MS.gene72127.t1	MTR_2g094270	64.259	263	74	3	1	256	1	250	3.95e-119	342
MS.gene72127.t1	MTR_1g095070	77.838	185	40	1	96	280	23	206	1.04e-102	299
MS.gene72127.t1	MTR_1g095070	77.640	161	35	1	96	256	23	182	5.59e-87	258
MS.gene72127.t1	MTR_4g059390	64.583	192	61	2	25	210	12	202	1.20e-86	258
MS.gene72127.t1	MTR_1g077540	36.638	232	128	7	52	278	21	238	5.98e-33	122
MS.gene72127.t1	MTR_2g101370	35.897	234	135	5	47	277	15	236	3.16e-32	119
MS.gene72127.t1	MTR_6g069040	38.723	235	128	7	52	282	21	243	1.03e-31	118
MS.gene72127.t1	MTR_4g063090	34.034	238	142	5	41	275	10	235	5.50e-30	114
MS.gene72127.t1	MTR_8g013680	35.470	234	134	6	46	274	11	232	9.23e-30	113
MS.gene72127.t1	MTR_1g115405	34.426	244	140	7	41	278	10	239	6.06e-29	111
MS.gene72127.t1	MTR_7g103030	33.333	243	138	7	42	277	11	236	4.89e-28	108
MS.gene72127.t1	MTR_7g023350	33.884	242	144	6	41	278	10	239	9.76e-28	107
MS.gene72127.t1	MTR_5g012810	36.726	226	127	7	52	274	19	231	9.34e-27	105
MS.gene72127.t1	MTR_1g006490	33.333	231	127	7	52	275	19	229	2.90e-26	103
MS.gene72127.t1	MTR_1g006590	33.906	233	123	8	52	275	19	229	2.07e-25	101
MS.gene72127.t1	MTR_2g060360	31.646	237	132	8	52	278	19	235	5.71e-20	87.0
MS.gene72127.t1	MTR_5g063930	30.263	228	144	6	47	274	32	244	1.39e-19	86.3
MS.gene72127.t1	MTR_8g087710	28.014	282	174	9	11	287	2	259	1.49e-19	86.3
MS.gene72127.t1	MTR_4g084880	26.549	226	146	5	55	277	38	246	8.61e-18	81.6
MS.gene72127.t1	MTR_5g079570	28.646	192	124	4	84	275	68	246	1.84e-17	80.5
MS.gene72127.t1	MTR_2g017660	27.391	230	145	6	41	267	28	238	3.95e-17	79.7
MS.gene72127.t1	MTR_2g017590	31.944	216	125	6	55	267	39	235	5.26e-17	79.3
MS.gene72127.t1	MTR_8g087720	27.917	240	156	6	49	287	35	258	1.04e-16	78.6
MS.gene72127.t1	MTR_4g006730	28.205	234	140	8	34	267	68	273	2.37e-16	77.8
MS.gene72127.t1	MTR_4g075570	27.692	195	119	7	99	279	95	281	3.19e-16	77.8
MS.gene72127.t1	MTR_2g104940	30.986	213	128	7	56	267	84	278	4.15e-16	77.4
MS.gene72127.t1	MTR_7g010650	28.390	236	149	7	50	284	35	251	5.79e-16	76.3
MS.gene72127.t1	MTR_3g008920	29.688	192	122	4	84	275	54	232	5.90e-16	76.3
MS.gene72127.t1	MTR_2g017610	29.577	213	134	5	55	267	41	237	7.25e-16	76.3
MS.gene72127.t1	MTR_2g017620	30.736	231	141	8	50	279	36	248	1.25e-15	75.5
MS.gene72127.t1	MTR_2g017620	30.736	231	141	8	50	279	22	234	1.28e-15	75.1
MS.gene72127.t1	MTR_4g075590	28.125	192	122	5	99	279	81	267	1.40e-15	75.9
MS.gene72127.t1	MTR_2g017570	29.577	213	134	5	55	267	41	237	1.53e-15	75.1
MS.gene72127.t1	MTR_1g097840	29.204	226	140	8	56	279	78	285	3.04e-13	68.9
MS.gene72127.t1	MTR_4g006630	28.492	179	114	4	89	267	22	186	4.70e-13	67.4
MS.gene72127.t1	MTR_4g006730	28.492	179	114	4	89	267	22	186	4.99e-13	67.4
MS.gene72127.t1	MTR_1g070385	25.778	225	148	6	49	272	59	265	3.50e-12	67.0
MS.gene72127.t1	MTR_2g017620	30.769	182	106	5	101	279	3	167	4.59e-12	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72127.t1	AT4G00430	85.263	285	41	1	3	287	4	287	0.0	504
MS.gene72127.t1	AT2G45960	86.316	285	37	2	3	287	4	286	0.0	502
MS.gene72127.t1	AT4G23400	87.719	285	34	1	3	287	4	287	9.38e-175	484
MS.gene72127.t1	AT1G01620	87.018	285	35	2	3	287	4	286	5.82e-173	479
MS.gene72127.t1	AT3G61430	85.965	285	38	2	3	287	4	286	2.01e-171	476
MS.gene72127.t1	AT3G61430	85.965	285	38	2	3	287	4	286	2.01e-171	476
MS.gene72127.t1	AT2G45960	86.614	254	32	2	3	256	4	255	6.17e-161	448
MS.gene72127.t1	AT2G45960	86.614	254	32	2	3	256	4	255	1.64e-159	446
MS.gene72127.t1	AT2G37170	71.538	260	67	3	27	280	55	313	3.72e-134	383
MS.gene72127.t1	AT4G35100	71.875	256	71	1	29	284	15	269	5.06e-134	380
MS.gene72127.t1	AT4G35100	71.875	256	71	1	29	284	15	269	5.06e-134	380
MS.gene72127.t1	AT1G01620	86.512	215	28	1	73	287	1	214	5.56e-134	378
MS.gene72127.t1	AT2G37170	71.538	260	67	3	27	280	12	270	9.03e-134	380
MS.gene72127.t1	AT3G53420	71.154	260	68	2	27	280	14	272	1.06e-133	380
MS.gene72127.t1	AT3G53420	71.154	260	68	2	27	280	14	272	1.06e-133	380
MS.gene72127.t1	AT2G37180	71.538	260	67	3	27	280	12	270	1.14e-133	380
MS.gene72127.t1	AT2G16850	71.875	256	71	1	29	284	13	267	5.18e-133	378
MS.gene72127.t1	AT5G60660	70.342	263	71	2	28	284	15	276	1.49e-132	377
MS.gene72127.t1	AT3G54820	67.778	270	80	2	17	280	3	271	4.08e-132	376
MS.gene72127.t1	AT2G39010	65.950	279	82	3	12	284	4	275	6.06e-131	373
MS.gene72127.t1	AT4G00430	82.547	212	36	1	3	214	4	214	2.86e-127	361
MS.gene72127.t1	AT2G39010	63.000	200	67	2	17	210	3	201	2.53e-84	252
MS.gene72127.t1	AT4G01470	35.146	239	140	5	42	277	11	237	2.51e-32	120
MS.gene72127.t1	AT1G73190	35.656	244	128	8	52	282	24	251	1.70e-31	118
MS.gene72127.t1	AT1G17810	36.596	235	129	5	52	278	24	246	2.51e-31	117
MS.gene72127.t1	AT5G47450	37.021	235	133	7	43	274	10	232	1.91e-30	115
MS.gene72127.t1	AT3G16240	35.931	231	133	6	47	274	14	232	6.86e-30	113
MS.gene72127.t1	AT3G26520	33.624	229	137	5	52	277	22	238	8.61e-29	110
MS.gene72127.t1	AT2G36830	33.469	245	136	8	41	276	10	236	4.72e-28	108
MS.gene72127.t1	AT4G17340	36.170	235	135	7	43	274	10	232	3.08e-27	106
MS.gene72127.t1	AT2G25810	33.478	230	135	8	52	278	19	233	3.10e-24	98.6
MS.gene72127.t1	AT3G47440	31.687	243	135	8	47	278	18	240	4.29e-24	98.6
MS.gene72127.t1	AT4G19030	26.712	292	185	8	3	287	13	282	3.84e-19	85.9
MS.gene72127.t1	AT1G31885	31.111	225	142	7	45	267	36	249	3.96e-18	83.2
MS.gene72127.t1	AT5G37820	28.571	217	129	7	55	266	46	241	2.55e-17	80.5
MS.gene72127.t1	AT5G37820	28.571	217	129	7	55	266	82	277	2.85e-17	80.9
MS.gene72127.t1	AT4G18910	27.027	259	165	8	38	287	36	279	1.52e-15	75.5
MS.gene72127.t1	AT2G34390	26.336	262	162	9	22	277	27	263	1.98e-15	75.1
MS.gene72127.t1	AT1G31885	31.351	185	117	5	85	267	19	195	5.42e-15	73.6
MS.gene72127.t1	AT3G06100	29.707	239	140	8	52	286	46	260	1.64e-14	72.4
MS.gene72127.t1	AT3G06100	29.661	236	138	8	55	286	1	212	4.13e-14	70.5
MS.gene72127.t1	AT2G34390	27.074	229	145	8	55	277	9	221	1.20e-13	69.7
MS.gene72127.t1	AT4G10380	26.022	269	166	8	21	273	32	283	2.18e-13	69.7
MS.gene72127.t1	AT5G37810	26.923	234	154	7	55	287	46	263	3.02e-13	68.9

Find 77 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAAGGTATTGCTTGGTCTTT+TGG	0.121056	4.2:-6331624	MS.gene72127:CDS
CATTGGGTTTGCTGTGTTCT+TGG	0.191867	4.2:-6330810	MS.gene72127:CDS
TTCTGGGTTGGACCATTTAT+TGG	0.216605	4.2:-6330576	MS.gene72127:CDS
GCAAGAGCAGCTCCAATAAA+TGG	0.282926	4.2:+6330564	None:intergenic
GAGCTGCCATCATCTTTAAC+AGG	0.292037	4.2:-6330730	MS.gene72127:CDS
GCTAGGTATGAACTGTTTAA+AGG	0.293383	4.2:-6331329	MS.gene72127:CDS
TACTGCACTGCTGGAATATC+AGG	0.324491	4.2:-6331579	MS.gene72127:intron
TACACCCATGACAGTCAAAA+TGG	0.345761	4.2:+6331683	None:intergenic
TGTTCCTCTGTTGGCATTCA+AGG	0.347490	4.2:-6331642	MS.gene72127:CDS
TGCTGTGTTCTTGGTCCACT+TGG	0.359026	4.2:-6330801	MS.gene72127:CDS
CTTTGGGCTGCTGTCCCTAA+TGG	0.359259	4.2:+6331831	None:intergenic
GCCCTTGTCTACTGCACTGC+TGG	0.363459	4.2:-6331588	MS.gene72127:CDS
GTAGTCTTTGCTGTCACTTT+GGG	0.366503	4.2:+6331815	None:intergenic
TTAATGCCTGTTCCTGTGAT+TGG	0.393415	4.2:+6330770	None:intergenic
ATAAACCCTGCTGTGACATT+TGG	0.394638	4.2:-6331461	MS.gene72127:CDS
TCTTTAACAGGGACTTTGCT+TGG	0.396893	4.2:-6330718	MS.gene72127:CDS
TCTTGGCTCCTCTTCCCATT+GGG	0.400657	4.2:-6330826	MS.gene72127:CDS
TTCTCAGAGAGGCAGCCATT+AGG	0.401275	4.2:-6331846	MS.gene72127:CDS
CTTGGTGCTATTTGTGGTGC+TGG	0.401687	4.2:-6331377	MS.gene72127:CDS
CAATGTCTTGGTGCTATTTG+TGG	0.402335	4.2:-6331383	MS.gene72127:CDS
AATGCCAACAGAGGAACATT+TGG	0.407248	4.2:+6331647	None:intergenic
TCTCAGAGAGGCAGCCATTA+GGG	0.408528	4.2:-6331845	MS.gene72127:CDS
AAGGAGGAAGATGTGAAAGT+TGG	0.409979	4.2:-6331879	MS.gene72127:CDS
ATCTTGGCTCCTCTTCCCAT+TGG	0.412469	4.2:-6330827	MS.gene72127:CDS
TGTAGTCTTTGCTGTCACTT+TGG	0.414094	4.2:+6331814	None:intergenic
CTTTAACAGGGACTTTGCTT+GGG	0.419659	4.2:-6330717	MS.gene72127:CDS
ACCATTCCAATCACAGGAAC+AGG	0.439708	4.2:-6330776	MS.gene72127:CDS
GAATGGCTCGGATGACAATC+TGG	0.440088	4.2:+6330529	None:intergenic
TTGTGGTGCTGGTGTGGTGA+AGG	0.443603	4.2:-6331366	MS.gene72127:CDS
TTGGCATTCAAGGTATTGCT+TGG	0.445934	4.2:-6331632	MS.gene72127:CDS
CCATCACCTTTAGTGTATCC+AGG	0.469714	4.2:+6331287	None:intergenic
TGTGGTGCTGGTGTGGTGAA+GGG	0.479619	4.2:-6331365	MS.gene72127:CDS
CCAGCTCCATTCTTTGAACC+TGG	0.482414	4.2:-6331780	MS.gene72127:CDS
GTGAATCCTGGATACACTAA+AGG	0.482977	4.2:-6331293	MS.gene72127:CDS
GACCATGACTTGAGCTCACC+AGG	0.486918	4.2:+6331762	None:intergenic
AGCTCACCAGGTTCAAAGAA+TGG	0.499372	4.2:+6331774	None:intergenic
AGAGTCTCTAGCATTTCTCT+TGG	0.499778	4.2:+6331202	None:intergenic
AAAGTCCCTGTTAAAGATGA+TGG	0.503711	4.2:+6330724	None:intergenic
AGAGAGAGTAAGAATGGAGA+AGG	0.505737	4.2:-6331898	None:intergenic
CAGTCAAAATGGTGATGTAA+AGG	0.509320	4.2:+6331694	None:intergenic
AACAGACCAAATGTCACAGC+AGG	0.509644	4.2:+6331455	None:intergenic
ACCTCCAAATGTTCCTCTGT+TGG	0.509764	4.2:-6331651	MS.gene72127:CDS
GAGAGTAAGAATGGAGAAGG+AGG	0.517379	4.2:-6331895	None:intergenic
GCAGCTCCAAGACTCCTAGC+TGG	0.521224	4.2:+6330746	None:intergenic
GCCAACAGAGGAACATTTGG+AGG	0.524215	4.2:+6331650	None:intergenic
TGCTATTTGTGGTGCTGGTG+TGG	0.526494	4.2:-6331372	MS.gene72127:CDS
ATTAACCCAGCTAGGAGTCT+TGG	0.528660	4.2:-6330752	MS.gene72127:CDS
TTCCAGCAGTGCAGTAGACA+AGG	0.538007	4.2:+6331586	None:intergenic
GGACTTGGAGCTGAGATTGT+TGG	0.540230	4.2:-6331266	MS.gene72127:CDS
AAGAACACAGCAAACCCAAT+GGG	0.541932	4.2:+6330812	None:intergenic
TCAAAGAATGGAGCTGGTGG+TGG	0.542478	4.2:+6331786	None:intergenic
CCTCAGGTCCTTGTCTTGAA+AGG	0.542853	4.2:+6330507	None:intergenic
CCAGGTTCAAAGAATGGAGC+TGG	0.545752	4.2:+6331780	None:intergenic
ACAGCAAACCCAATGGGAAG+AGG	0.548821	4.2:+6330818	None:intergenic
TTGCAGTGGATATTCTGGGT+TGG	0.548834	4.2:-6330588	MS.gene72127:intron
TACACTAAAGGTGATGGACT+TGG	0.556791	4.2:-6331281	MS.gene72127:CDS
CCTGGATACACTAAAGGTGA+TGG	0.566370	4.2:-6331287	MS.gene72127:CDS
CAAGAACACAGCAAACCCAA+TGG	0.568082	4.2:+6330811	None:intergenic
TACATTGTGATGCAATGTCT+TGG	0.571531	4.2:-6331395	MS.gene72127:CDS
GATGTAAAGGAACAAGAAAG+TGG	0.573621	4.2:+6331707	None:intergenic
TTGTCTTGAAAGGAATGGCT+CGG	0.574695	4.2:+6330517	None:intergenic
GGTCCTTGTCTTGAAAGGAA+TGG	0.577153	4.2:+6330512	None:intergenic
CAGCTCCAAGACTCCTAGCT+GGG	0.583912	4.2:+6330747	None:intergenic
TTGGCTACCATTCCAATCAC+AGG	0.604120	4.2:-6330782	MS.gene72127:CDS
AACCTGGTGAGCTCAAGTCA+TGG	0.607189	4.2:-6331764	MS.gene72127:CDS
AGCTGCCATCATCTTTAACA+GGG	0.632590	4.2:-6330729	MS.gene72127:CDS
CCTTTCAAGACAAGGACCTG+AGG	0.635312	4.2:-6330507	None:intergenic
GCCTGTTCCTGTGATTGGAA+TGG	0.635477	4.2:+6330775	None:intergenic
GAACAGGCATTAACCCAGCT+AGG	0.644423	4.2:-6330760	MS.gene72127:CDS
TCCAGCAGTGCAGTAGACAA+GGG	0.651759	4.2:+6331587	None:intergenic
GGTTCAAAGAATGGAGCTGG+TGG	0.653748	4.2:+6331783	None:intergenic
GAGCCATTCCTTTCAAGACA+AGG	0.654804	4.2:-6330515	MS.gene72127:CDS
AATACCTTGAATGCCAACAG+AGG	0.688113	4.2:+6331638	None:intergenic
ACAGACCAAATGTCACAGCA+GGG	0.700234	4.2:+6331456	None:intergenic
GAGCAAACAAATTCTCAGAG+AGG	0.712701	4.2:-6331857	MS.gene72127:CDS
AGGTATGAACTGTTTAAAGG+TGG	0.726401	4.2:-6331326	MS.gene72127:CDS
TGATTGGAATGGTAGCCAAG+TGG	0.737998	4.2:+6330786	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAATTTTGTTCATAAAAATA+AGG	+	chr4.2:6331726-6331745	None:intergenic	10.0%
!!	AAGAAATCAAAAAATAGAAA+AGG	+	chr4.2:6331241-6331260	None:intergenic	15.0%
!!!	ACTTATAATTTATTTTCCTA+AGG	+	chr4.2:6331294-6331313	None:intergenic	15.0%
!!!	CTATATATTTTAGTTATTTG+TGG	-	chr4.2:6331389-6331408	MS.gene72127:CDS	15.0%
!	ATGTTAATAGCCAAGAATAA+TGG	-	chr4.2:6330877-6330896	MS.gene72127:intron	25.0%
!	CAAAGTTAAAAAGTCCTAAT+TGG	+	chr4.2:6331341-6331360	None:intergenic	25.0%
!	TTCTAACTCTAATTTCAATG+TGG	-	chr4.2:6331521-6331540	MS.gene72127:intron	25.0%
!!	TCAATGTGGTTATTCTTTTT+GGG	-	chr4.2:6331535-6331554	MS.gene72127:intron	25.0%
!!	TTCAATGTGGTTATTCTTTT+TGG	-	chr4.2:6331534-6331553	MS.gene72127:intron	25.0%
!!!	CAATTAGGACTTTTTAACTT+TGG	-	chr4.2:6331339-6331358	MS.gene72127:CDS	25.0%
!!!	CATTTTTTTTCCTTCCAATT+AGG	-	chr4.2:6331324-6331343	MS.gene72127:CDS	25.0%
	AAAAGGAAATGAAGACATAC+AGG	+	chr4.2:6331224-6331243	None:intergenic	30.0%
	AAGGTGATAGAAAACATACA+TGG	+	chr4.2:6331707-6331726	None:intergenic	30.0%
	GTTAAAAAGTCCTAATTGGA+AGG	+	chr4.2:6331337-6331356	None:intergenic	30.0%
	TTTCTGCATATACTGGTATA+TGG	-	chr4.2:6331468-6331487	MS.gene72127:CDS	30.0%
!	CAATGTGGTTATTCTTTTTG+GGG	-	chr4.2:6331536-6331555	MS.gene72127:intron	30.0%
!	GATCTATTTTCTGCATATAC+TGG	-	chr4.2:6331461-6331480	MS.gene72127:CDS	30.0%
!!	TTTTGGGGAATTATAGATCT+TGG	-	chr4.2:6331551-6331570	MS.gene72127:intron	30.0%
!!!	TTATTGATGATGTTTTGCAG+TGG	-	chr4.2:6331792-6331811	MS.gene72127:CDS	30.0%
	AAAGTCCCTGTTAAAGATGA+TGG	+	chr4.2:6331673-6331692	None:intergenic	35.0%
	AGGTATGAACTGTTTAAAGG+TGG	-	chr4.2:6331068-6331087	MS.gene72127:intron	35.0%
	CACAGATCTACCATTATTCT+TGG	+	chr4.2:6330890-6330909	None:intergenic	35.0%
	GATGTAAAGGAACAAGAAAG+TGG	+	chr4.2:6330690-6330709	None:intergenic	35.0%
	GCTAGGTATGAACTGTTTAA+AGG	-	chr4.2:6331065-6331084	MS.gene72127:intron	35.0%
	TACATTGTGATGCAATGTCT+TGG	-	chr4.2:6330999-6331018	MS.gene72127:intron	35.0%
!	ATGTTTTGCAGTGGATATTC+TGG	-	chr4.2:6331801-6331820	MS.gene72127:CDS	35.0%
!	CAGTCAAAATGGTGATGTAA+AGG	+	chr4.2:6330703-6330722	None:intergenic	35.0%
!	TGTGACATTTGGTCTGTTTT+TGG	-	chr4.2:6330944-6330963	MS.gene72127:intron	35.0%
!	TGTTTTGCAGTGGATATTCT+GGG	-	chr4.2:6331802-6331821	MS.gene72127:CDS	35.0%
!!	ATCTGTGTGTTGTTTGTGAT+AGG	-	chr4.2:6330903-6330922	MS.gene72127:intron	35.0%
!!!	AAGGTATTGCTTGGTCTTTT+GGG	-	chr4.2:6330771-6330790	MS.gene72127:CDS	35.0%
!!!	ATCACCATTTTGACTGTCAT+GGG	-	chr4.2:6330707-6330726	MS.gene72127:intron	35.0%
	AAGAACACAGCAAACCCAAT+GGG	+	chr4.2:6331585-6331604	None:intergenic	40.0%
	AAGGAGGAAGATGTGAAAGT+TGG	-	chr4.2:6330515-6330534	MS.gene72127:CDS	40.0%
	AATACCTTGAATGCCAACAG+AGG	+	chr4.2:6330759-6330778	None:intergenic	40.0%
	AATGCCAACAGAGGAACATT+TGG	+	chr4.2:6330750-6330769	None:intergenic	40.0%
	AGAGTCTCTAGCATTTCTCT+TGG	+	chr4.2:6331195-6331214	None:intergenic	40.0%
	AGCTGCCATCATCTTTAACA+GGG	-	chr4.2:6331665-6331684	MS.gene72127:CDS	40.0%
	ATAAACCCTGCTGTGACATT+TGG	-	chr4.2:6330933-6330952	MS.gene72127:intron	40.0%
	CTTTAACAGGGACTTTGCTT+GGG	-	chr4.2:6331677-6331696	MS.gene72127:CDS	40.0%
	GAGCAAACAAATTCTCAGAG+AGG	-	chr4.2:6330537-6330556	MS.gene72127:CDS	40.0%
	GTGAATCCTGGATACACTAA+AGG	-	chr4.2:6331101-6331120	MS.gene72127:intron	40.0%
	TACACCCATGACAGTCAAAA+TGG	+	chr4.2:6330714-6330733	None:intergenic	40.0%
	TCTTTAACAGGGACTTTGCT+TGG	-	chr4.2:6331676-6331695	MS.gene72127:CDS	40.0%
	TTAATGCCTGTTCCTGTGAT+TGG	+	chr4.2:6331627-6331646	None:intergenic	40.0%
	TTGTCTTGAAAGGAATGGCT+CGG	+	chr4.2:6331880-6331899	None:intergenic	40.0%
!	GGTGCTAATTTTGTGAATCC+TGG	-	chr4.2:6331089-6331108	MS.gene72127:intron	40.0%
!	GTGTGCTGAAAGTTTTCCTT+AGG	-	chr4.2:6331275-6331294	MS.gene72127:CDS	40.0%
!	TCATGGTCTTTTTACAGAGC+TGG	-	chr4.2:6330647-6330666	MS.gene72127:intron	40.0%
!	TGTAGTCTTTGCTGTCACTT+TGG	+	chr4.2:6330583-6330602	None:intergenic	40.0%
!	TTCTGGGTTGGACCATTTAT+TGG	-	chr4.2:6331818-6331837	MS.gene72127:CDS	40.0%
!!	AGGTATTGCTTGGTCTTTTG+GGG	-	chr4.2:6330772-6330791	MS.gene72127:CDS	40.0%
!!	CAATGTCTTGGTGCTATTTG+TGG	-	chr4.2:6331011-6331030	MS.gene72127:intron	40.0%
!!	GTAGTCTTTGCTGTCACTTT+GGG	+	chr4.2:6330582-6330601	None:intergenic	40.0%
!!	TACACTAAAGGTGATGGACT+TGG	-	chr4.2:6331113-6331132	MS.gene72127:intron	40.0%
!!	TGTGTGTTGTTTGTGATAGG+TGG	-	chr4.2:6330906-6330925	MS.gene72127:intron	40.0%
!!	TTGGCATTCAAGGTATTGCT+TGG	-	chr4.2:6330762-6330781	MS.gene72127:CDS	40.0%
!!!	AGGGTTTTGAAGGTAATGCT+AGG	-	chr4.2:6331048-6331067	MS.gene72127:intron	40.0%
!!!	CAAGGTATTGCTTGGTCTTT+TGG	-	chr4.2:6330770-6330789	MS.gene72127:CDS	40.0%
!!!	CATCACCATTTTGACTGTCA+TGG	-	chr4.2:6330706-6330725	MS.gene72127:intron	40.0%
!!!	CATTTGGTCTGTTTTTGGCA+AGG	-	chr4.2:6330949-6330968	MS.gene72127:intron	40.0%
	AACAGACCAAATGTCACAGC+AGG	+	chr4.2:6330942-6330961	None:intergenic	45.0%
	ACAGACCAAATGTCACAGCA+GGG	+	chr4.2:6330941-6330960	None:intergenic	45.0%
	ACCATTCCAATCACAGGAAC+AGG	-	chr4.2:6331618-6331637	MS.gene72127:CDS	45.0%
	ACCTCCAAATGTTCCTCTGT+TGG	-	chr4.2:6330743-6330762	MS.gene72127:CDS	45.0%
	AGCTCACCAGGTTCAAAGAA+TGG	+	chr4.2:6330623-6330642	None:intergenic	45.0%
	ATTAACCCAGCTAGGAGTCT+TGG	-	chr4.2:6331642-6331661	MS.gene72127:CDS	45.0%
	CAAGAACACAGCAAACCCAA+TGG	+	chr4.2:6331586-6331605	None:intergenic	45.0%
	CCATCACCTTTAGTGTATCC+AGG	+	chr4.2:6331110-6331129	None:intergenic	45.0%
	CCTGGATACACTAAAGGTGA+TGG	-	chr4.2:6331107-6331126	MS.gene72127:intron	45.0%
	GAGCCATTCCTTTCAAGACA+AGG	-	chr4.2:6331879-6331898	MS.gene72127:CDS	45.0%
	GAGCTGCCATCATCTTTAAC+AGG	-	chr4.2:6331664-6331683	MS.gene72127:CDS	45.0%
	GCAAGAGCAGCTCCAATAAA+TGG	+	chr4.2:6331833-6331852	None:intergenic	45.0%
	GGTCCTTGTCTTGAAAGGAA+TGG	+	chr4.2:6331885-6331904	None:intergenic	45.0%
	TACTGCACTGCTGGAATATC+AGG	-	chr4.2:6330815-6330834	MS.gene72127:CDS	45.0%
	TGATTGGAATGGTAGCCAAG+TGG	+	chr4.2:6331611-6331630	None:intergenic	45.0%
	TGTTCCTCTGTTGGCATTCA+AGG	-	chr4.2:6330752-6330771	MS.gene72127:CDS	45.0%
	TTGCAGTGGATATTCTGGGT+TGG	-	chr4.2:6331806-6331825	MS.gene72127:CDS	45.0%
	TTGGCTACCATTCCAATCAC+AGG	-	chr4.2:6331612-6331631	MS.gene72127:CDS	45.0%
!!	CATTGGGTTTGCTGTGTTCT+TGG	-	chr4.2:6331584-6331603	MS.gene72127:CDS	45.0%
!!	GGTATTGCTTGGTCTTTTGG+GGG	-	chr4.2:6330773-6330792	MS.gene72127:CDS	45.0%
!!	AAAAATTAAATAATATTGTA+AGG	+	chr4.2:6330847-6330866	None:intergenic	5.0%
	AACCTGGTGAGCTCAAGTCA+TGG	-	chr4.2:6330630-6330649	MS.gene72127:intron	50.0%
	ACAGCAAACCCAATGGGAAG+AGG	+	chr4.2:6331579-6331598	None:intergenic	50.0%
	ATCTTGGCTCCTCTTCCCAT+TGG	-	chr4.2:6331567-6331586	MS.gene72127:intron	50.0%
	CCAGCTCCATTCTTTGAACC+TGG	-	chr4.2:6330614-6330633	MS.gene72127:intron	50.0%
	CCAGGTTCAAAGAATGGAGC+TGG	+	chr4.2:6330617-6330636	None:intergenic	50.0%
	GAACAGGCATTAACCCAGCT+AGG	-	chr4.2:6331634-6331653	MS.gene72127:CDS	50.0%
	GCCAACAGAGGAACATTTGG+AGG	+	chr4.2:6330747-6330766	None:intergenic	50.0%
	GCCTGTTCCTGTGATTGGAA+TGG	+	chr4.2:6331622-6331641	None:intergenic	50.0%
	GGACTTGGAGCTGAGATTGT+TGG	-	chr4.2:6331128-6331147	MS.gene72127:intron	50.0%
	GGTTCAAAGAATGGAGCTGG+TGG	+	chr4.2:6330614-6330633	None:intergenic	50.0%
	TCAAAGAATGGAGCTGGTGG+TGG	+	chr4.2:6330611-6330630	None:intergenic	50.0%
	TCCAGCAGTGCAGTAGACAA+GGG	+	chr4.2:6330810-6330829	None:intergenic	50.0%
	TCTCAGAGAGGCAGCCATTA+GGG	-	chr4.2:6330549-6330568	MS.gene72127:CDS	50.0%
	TCTTGGCTCCTCTTCCCATT+GGG	-	chr4.2:6331568-6331587	MS.gene72127:intron	50.0%
	TGCTGTGTTCTTGGTCCACT+TGG	-	chr4.2:6331593-6331612	MS.gene72127:CDS	50.0%
	TTCCAGCAGTGCAGTAGACA+AGG	+	chr4.2:6330811-6330830	None:intergenic	50.0%
	TTCTCAGAGAGGCAGCCATT+AGG	-	chr4.2:6330548-6330567	MS.gene72127:CDS	50.0%
!	GAATGGCTCGGATGACAATC+TGG	+	chr4.2:6331868-6331887	None:intergenic	50.0%
!!	CTTGGTGCTATTTGTGGTGC+TGG	-	chr4.2:6331017-6331036	MS.gene72127:intron	50.0%
!!	GGTGTGGTGAAGGGTTTTGA+AGG	-	chr4.2:6331038-6331057	MS.gene72127:intron	50.0%
!!	TGCTATTTGTGGTGCTGGTG+TGG	-	chr4.2:6331022-6331041	MS.gene72127:intron	50.0%
	CAGCTCCAAGACTCCTAGCT+GGG	+	chr4.2:6331650-6331669	None:intergenic	55.0%
	CTTTGGGCTGCTGTCCCTAA+TGG	+	chr4.2:6330566-6330585	None:intergenic	55.0%
	GACCATGACTTGAGCTCACC+AGG	+	chr4.2:6330635-6330654	None:intergenic	55.0%
!!	TGTGGTGCTGGTGTGGTGAA+GGG	-	chr4.2:6331029-6331048	MS.gene72127:intron	55.0%
!!	TTGTGGTGCTGGTGTGGTGA+AGG	-	chr4.2:6331028-6331047	MS.gene72127:intron	55.0%
	GCAGCTCCAAGACTCCTAGC+TGG	+	chr4.2:6331651-6331670	None:intergenic	60.0%
	GCCCTTGTCTACTGCACTGC+TGG	-	chr4.2:6330806-6330825	MS.gene72127:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	6330509	6331907	6330509	ID=MS.gene72127
chr4.2	mRNA	6330509	6331907	6330509	ID=MS.gene72127.t1;Parent=MS.gene72127
chr4.2	exon	6331580	6331907	6331580	ID=MS.gene72127.t1.exon1;Parent=MS.gene72127.t1
chr4.2	CDS	6331580	6331907	6331580	ID=cds.MS.gene72127.t1;Parent=MS.gene72127.t1
chr4.2	exon	6331193	6331491	6331193	ID=MS.gene72127.t1.exon2;Parent=MS.gene72127.t1
chr4.2	CDS	6331193	6331491	6331193	ID=cds.MS.gene72127.t1;Parent=MS.gene72127.t1
chr4.2	exon	6330709	6330849	6330709	ID=MS.gene72127.t1.exon3;Parent=MS.gene72127.t1
chr4.2	CDS	6330709	6330849	6330709	ID=cds.MS.gene72127.t1;Parent=MS.gene72127.t1
chr4.2	exon	6330509	6330604	6330509	ID=MS.gene72127.t1.exon4;Parent=MS.gene72127.t1
chr4.2	CDS	6330509	6330604	6330509	ID=cds.MS.gene72127.t1;Parent=MS.gene72127.t1

Gene Sequence

>MS.gene72127

ATGGAGAAGGAGGAAGATGTGAAAGTTGGAGCAAACAAATTCTCAGAGAGGCAGCCATTAGGGACAGCAGCCCAAAGTGACAGCAAAGACTACAAAGAGCCACCACCAGCTCCATTCTTTGAACCTGGTGAGCTCAAGTCATGGTCTTTTTACAGAGCTGGAATTGCTGAGTTCATAGCCACTTTCTTGTTCCTTTACATCACCATTTTGACTGTCATGGGTGTAAACAGATCAACCTCCAAATGTTCCTCTGTTGGCATTCAAGGTATTGCTTGGTCTTTTGGGGGTATGATATTTGCCCTTGTCTACTGCACTGCTGGAATATCAGGTAAAAACCTTACAATATTATTTAATTTTTTTTCTTGAGAATGTTAATAGCCAAGAATAATGGTAGATCTGTGTGTTGTTTGTGATAGGTGGACACATAAACCCTGCTGTGACATTTGGTCTGTTTTTGGCAAGGAAGCTGTCACTTACAAGAGCTATATTCTACATTGTGATGCAATGTCTTGGTGCTATTTGTGGTGCTGGTGTGGTGAAGGGTTTTGAAGGTAATGCTAGGTATGAACTGTTTAAAGGTGGTGCTAATTTTGTGAATCCTGGATACACTAAAGGTGATGGACTTGGAGCTGAGATTGTTGGTACTTTTGTTCTTGTTTACACTGTTTTCTCTGCTACTGATGCCAAGAGAAATGCTAGAGACTCTCATGTTCCTGTATGTCTTCATTTCCTTTTCTATTTTTTGATTTCTTCATTCAATTTGCATGTGTGCTGAAAGTTTTCCTTAGGAAAATAAATTATAAGTTTACTCTTTACATTTTTTTTCCTTCCAATTAGGACTTTTTAACTTTGGTCTTTACATCAATTATATGACTTTAGTCTATATATTTTAGTTATTTGTGGTGATAATAAATGCAGTTATTTATTTAATCAATGAACTTAAAATATTGATGATCTATTTTCTGCATATACTGGTATATGGTTTTAATTTGTGAATTAATCTTAATGTTTATTCTAACTCTAATTTCAATGTGGTTATTCTTTTTGGGGAATTATAGATCTTGGCTCCTCTTCCCATTGGGTTTGCTGTGTTCTTGGTCCACTTGGCTACCATTCCAATCACAGGAACAGGCATTAACCCAGCTAGGAGTCTTGGAGCTGCCATCATCTTTAACAGGGACTTTGCTTGGGATGACCATGTATGTTTTCTATCACCTTATTTTTATGAACAAAATTTAGACTCTAATTTTTGTCTTTAGATTAAATAATTAATTAATACTGTATTATTGATGATGTTTTGCAGTGGATATTCTGGGTTGGACCATTTATTGGAGCTGCTCTTGCTGCTGTGTATCACCAGATTGTCATCCGAGCCATTCCTTTCAAGACAAGGACCTGA

Protein sequence

>MS.gene72127.t1

MEKEEDVKVGANKFSERQPLGTAAQSDSKDYKEPPPAPFFEPGELKSWSFYRAGIAEFIATFLFLYITILTVMGVNRSTSKCSSVGIQGIAWSFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAIFYIVMQCLGAICGAGVVKGFEGNARYELFKGGANFVNPGYTKGDGLGAEIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIIFNRDFAWDDHWIFWVGPFIGAALAAVYHQIVIRAIPFKTRT