Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89702.t1 | XP_013447130.1 | 91.8 | 147 | 12 | 0 | 1 | 147 | 81 | 227 | 1.40E-68 | 268.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89702.t1 | A0A072TUP3 | 91.8 | 147 | 12 | 0 | 1 | 147 | 81 | 227 | 1.0e-68 | 268.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049413 | MS.gene89702 | 0.81659 | 5.11E-52 | -1.69E-46 |
| MS.gene050961 | MS.gene89702 | 0.839494 | 1.53E-57 | -1.69E-46 |
| MS.gene051699 | MS.gene89702 | 0.804372 | 2.23E-49 | -1.69E-46 |
| MS.gene051959 | MS.gene89702 | 0.870066 | 1.93E-66 | -1.69E-46 |
| MS.gene051960 | MS.gene89702 | 0.866078 | 3.71E-65 | -1.69E-46 |
| MS.gene052053 | MS.gene89702 | 0.854965 | 8.66E-62 | -1.69E-46 |
| MS.gene052533 | MS.gene89702 | 0.802913 | 4.48E-49 | -1.69E-46 |
| MS.gene052559 | MS.gene89702 | 0.839007 | 2.05E-57 | -1.69E-46 |
| MS.gene052560 | MS.gene89702 | 0.806517 | 7.92E-50 | -1.69E-46 |
| MS.gene052570 | MS.gene89702 | 0.819732 | 9.95E-53 | -1.69E-46 |
| MS.gene053602 | MS.gene89702 | 0.807164 | 5.78E-50 | -1.69E-46 |
| MS.gene053932 | MS.gene89702 | 0.833428 | 5.37E-56 | -1.69E-46 |
| MS.gene054892 | MS.gene89702 | 0.848639 | 5.39E-60 | -1.69E-46 |
| MS.gene054893 | MS.gene89702 | 0.853701 | 2.01E-61 | -1.69E-46 |
| MS.gene055150 | MS.gene89702 | 0.837531 | 4.92E-57 | -1.69E-46 |
| MS.gene055549 | MS.gene89702 | 0.822699 | 2.06E-53 | -1.69E-46 |
| MS.gene055550 | MS.gene89702 | 0.825429 | 4.71E-54 | -1.69E-46 |
| MS.gene055551 | MS.gene89702 | 0.814237 | 1.71E-51 | -1.69E-46 |
| MS.gene05624 | MS.gene89702 | 0.83036 | 3.07E-55 | -1.69E-46 |
| MS.gene057001 | MS.gene89702 | 0.828666 | 7.92E-55 | -1.69E-46 |
| MS.gene057093 | MS.gene89702 | 0.816211 | 6.21E-52 | -1.69E-46 |
| MS.gene057622 | MS.gene89702 | 0.809624 | 1.73E-50 | -1.69E-46 |
| MS.gene058095 | MS.gene89702 | 0.804386 | 2.22E-49 | -1.69E-46 |
| MS.gene058592 | MS.gene89702 | 0.839075 | 1.97E-57 | -1.69E-46 |
| MS.gene05878 | MS.gene89702 | 0.858707 | 6.85E-63 | -1.69E-46 |
| MS.gene05952 | MS.gene89702 | 0.865104 | 7.52E-65 | -1.69E-46 |
| MS.gene05956 | MS.gene89702 | 0.808627 | 2.82E-50 | -1.69E-46 |
| MS.gene05985 | MS.gene89702 | -0.807582 | 4.71E-50 | -1.69E-46 |
| MS.gene06023 | MS.gene89702 | 0.964911 | 6.31E-124 | -1.69E-46 |
| MS.gene060412 | MS.gene89702 | 0.819971 | 8.77E-53 | -1.69E-46 |
| MS.gene060413 | MS.gene89702 | 0.816207 | 6.23E-52 | -1.69E-46 |
| MS.gene060522 | MS.gene89702 | 0.811704 | 6.13E-51 | -1.69E-46 |
| MS.gene060964 | MS.gene89702 | 0.83844 | 2.87E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89702.t1 | MTR_8g099035 | 91.837 | 147 | 12 | 0 | 1 | 147 | 81 | 227 | 1.70e-95 | 275 |
| MS.gene89702.t1 | MTR_8g028475 | 38.393 | 112 | 68 | 1 | 19 | 130 | 93 | 203 | 2.80e-21 | 85.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89702.t1 | AT2G46150 | 34.513 | 113 | 73 | 1 | 19 | 130 | 93 | 205 | 3.52e-19 | 80.5 |
| MS.gene89702.t1 | AT3G54200 | 31.868 | 91 | 62 | 0 | 25 | 115 | 114 | 204 | 7.37e-11 | 58.5 |
Find 31 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTTGAAACTTCCACTTATA+AGG | 0.143507 | 4.4:+5042057 | None:intergenic |
| TATTGGATTCATTAATATTT+AGG | 0.155942 | 4.4:-5041649 | None:intergenic |
| ATACAAATTCATTCACTTTC+AGG | 0.174928 | 4.4:-5041983 | MS.gene89702:CDS |
| TTCTATAATGGAATTGATAT+TGG | 0.258144 | 4.4:-5041936 | MS.gene89702:CDS |
| AATATGTTCAAGAGGTCAAT+TGG | 0.340166 | 4.4:-5041738 | MS.gene89702:CDS |
| GTTGAAACTTCCACTTATAA+GGG | 0.363902 | 4.4:+5042058 | None:intergenic |
| ACACGTGGTGATACTTGTAT+TGG | 0.390721 | 4.4:-5041666 | MS.gene89702:CDS |
| AATTCATTCACTTTCAGGTA+TGG | 0.412565 | 4.4:-5041978 | MS.gene89702:CDS |
| AGTACTTATACGAAGATCAA+TGG | 0.434098 | 4.4:-5041777 | MS.gene89702:CDS |
| AGCTTGTGGACAATCAAGAT+TGG | 0.439651 | 4.4:-5041833 | MS.gene89702:CDS |
| ATATGTTCAAGAGGTCAATT+GGG | 0.448508 | 4.4:-5041737 | MS.gene89702:CDS |
| AATAGTGTTACCCTTATAAG+TGG | 0.474862 | 4.4:-5042068 | MS.gene89702:CDS |
| ATTCATTCACTTTCAGGTAT+GGG | 0.482613 | 4.4:-5041977 | MS.gene89702:CDS |
| TATAATGGAATTGATATTGG+AGG | 0.495203 | 4.4:-5041933 | MS.gene89702:CDS |
| AGTACATACGATATCCCAAC+AGG | 0.525073 | 4.4:-5041696 | MS.gene89702:CDS |
| AAAAGCTAAGGCGCAAAGAA+CGG | 0.530162 | 4.4:-5041892 | MS.gene89702:CDS |
| CACGTGTGATTGAACCTGTT+GGG | 0.532056 | 4.4:+5041682 | None:intergenic |
| TTCATTCACTTTCAGGTATG+GGG | 0.551722 | 4.4:-5041976 | MS.gene89702:CDS |
| AATGGAATTGATATTGGAGG+AGG | 0.560744 | 4.4:-5041930 | MS.gene89702:CDS |
| CTTATAAGTGGAAGTTTCAA+CGG | 0.570737 | 4.4:-5042056 | MS.gene89702:CDS |
| TGGAGGAGGAATACAACCAC+CGG | 0.577353 | 4.4:-5041916 | MS.gene89702:CDS |
| ACAACCACCGGGAAAAGCTA+AGG | 0.582230 | 4.4:-5041904 | MS.gene89702:CDS |
| AAATATTGAATATGTTCAAG+AGG | 0.596331 | 4.4:-5041746 | MS.gene89702:CDS |
| CCACGTGTGATTGAACCTGT+TGG | 0.609505 | 4.4:+5041681 | None:intergenic |
| CTAAGGCGCAAAGAACGGCA+AGG | 0.613584 | 4.4:-5041887 | MS.gene89702:CDS |
| GGCAAGGGTAAACGTGACAT+TGG | 0.620256 | 4.4:-5041871 | MS.gene89702:CDS |
| TAAGGCGCAAAGAACGGCAA+GGG | 0.636962 | 4.4:-5041886 | MS.gene89702:CDS |
| TATGTTCAAGAGGTCAATTG+GGG | 0.679448 | 4.4:-5041736 | MS.gene89702:CDS |
| GGAGGAGGAATACAACCACC+GGG | 0.691763 | 4.4:-5041915 | MS.gene89702:CDS |
| CCAACAGGTTCAATCACACG+TGG | 0.713111 | 4.4:-5041681 | MS.gene89702:CDS |
| AATTCCATTATAGAAAACAG+TGG | 0.718426 | 4.4:+5041944 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATATTGAATATGTTCAAG+AGG | - | chr4.4:5041975-5041994 | MS.gene89702:CDS | 20.0% |
| !!! | TTCTATAATGGAATTGATAT+TGG | - | chr4.4:5041785-5041804 | MS.gene89702:CDS | 20.0% |
| ! | AATTCCATTATAGAAAACAG+TGG | + | chr4.4:5041780-5041799 | None:intergenic | 25.0% |
| ! | ATACAAATTCATTCACTTTC+AGG | - | chr4.4:5041738-5041757 | MS.gene89702:CDS | 25.0% |
| !! | TATAATGGAATTGATATTGG+AGG | - | chr4.4:5041788-5041807 | MS.gene89702:CDS | 25.0% |
| AATAGTGTTACCCTTATAAG+TGG | - | chr4.4:5041653-5041672 | MS.gene89702:CDS | 30.0% | |
| AATATGTTCAAGAGGTCAAT+TGG | - | chr4.4:5041983-5042002 | MS.gene89702:CDS | 30.0% | |
| AGTACTTATACGAAGATCAA+TGG | - | chr4.4:5041944-5041963 | MS.gene89702:CDS | 30.0% | |
| ATATGTTCAAGAGGTCAATT+GGG | - | chr4.4:5041984-5042003 | MS.gene89702:CDS | 30.0% | |
| CTTATAAGTGGAAGTTTCAA+CGG | - | chr4.4:5041665-5041684 | MS.gene89702:CDS | 30.0% | |
| GTTGAAACTTCCACTTATAA+GGG | + | chr4.4:5041666-5041685 | None:intergenic | 30.0% | |
| ! | AACACCACTGTTTTCTATAA+TGG | - | chr4.4:5041773-5041792 | MS.gene89702:CDS | 30.0% |
| ! | AATTCATTCACTTTCAGGTA+TGG | - | chr4.4:5041743-5041762 | MS.gene89702:CDS | 30.0% |
| ! | ATTCATTCACTTTCAGGTAT+GGG | - | chr4.4:5041744-5041763 | MS.gene89702:CDS | 30.0% |
| AATGGAATTGATATTGGAGG+AGG | - | chr4.4:5041791-5041810 | MS.gene89702:CDS | 35.0% | |
| CGTTGAAACTTCCACTTATA+AGG | + | chr4.4:5041667-5041686 | None:intergenic | 35.0% | |
| GATTAATGCGAAAAAGCTTG+TGG | - | chr4.4:5041874-5041893 | MS.gene89702:CDS | 35.0% | |
| TATGTTCAAGAGGTCAATTG+GGG | - | chr4.4:5041985-5042004 | MS.gene89702:CDS | 35.0% | |
| ! | TTCATTCACTTTCAGGTATG+GGG | - | chr4.4:5041745-5041764 | MS.gene89702:CDS | 35.0% |
| AAAAGCTAAGGCGCAAAGAA+CGG | - | chr4.4:5041829-5041848 | MS.gene89702:CDS | 40.0% | |
| ACACGTGGTGATACTTGTAT+TGG | - | chr4.4:5042055-5042074 | MS.gene89702:CDS | 40.0% | |
| AGCTTGTGGACAATCAAGAT+TGG | - | chr4.4:5041888-5041907 | MS.gene89702:CDS | 40.0% | |
| AGTACATACGATATCCCAAC+AGG | - | chr4.4:5042025-5042044 | MS.gene89702:CDS | 40.0% | |
| CACGTGTGATTGAACCTGTT+GGG | + | chr4.4:5042042-5042061 | None:intergenic | 45.0% | |
| ACAACCACCGGGAAAAGCTA+AGG | - | chr4.4:5041817-5041836 | MS.gene89702:CDS | 50.0% | |
| CCAACAGGTTCAATCACACG+TGG | - | chr4.4:5042040-5042059 | MS.gene89702:CDS | 50.0% | |
| CCACGTGTGATTGAACCTGT+TGG | + | chr4.4:5042043-5042062 | None:intergenic | 50.0% | |
| GGCAAGGGTAAACGTGACAT+TGG | - | chr4.4:5041850-5041869 | MS.gene89702:CDS | 50.0% | |
| TAAGGCGCAAAGAACGGCAA+GGG | - | chr4.4:5041835-5041854 | MS.gene89702:CDS | 50.0% | |
| TGGAGGAGGAATACAACCAC+CGG | - | chr4.4:5041805-5041824 | MS.gene89702:CDS | 50.0% | |
| !! | CTTTGCGCCTTAGCTTTTCC+CGG | + | chr4.4:5041827-5041846 | None:intergenic | 50.0% |
| CTAAGGCGCAAAGAACGGCA+AGG | - | chr4.4:5041834-5041853 | MS.gene89702:CDS | 55.0% | |
| GGAGGAGGAATACAACCACC+GGG | - | chr4.4:5041806-5041825 | MS.gene89702:CDS | 55.0% | |
| !! | TGCGCCTTAGCTTTTCCCGG+TGG | + | chr4.4:5041824-5041843 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 5041650 | 5042093 | 5041650 | ID=MS.gene89702 |
| chr4.4 | mRNA | 5041650 | 5042093 | 5041650 | ID=MS.gene89702.t1;Parent=MS.gene89702 |
| chr4.4 | exon | 5041650 | 5042093 | 5041650 | ID=MS.gene89702.t1.exon1;Parent=MS.gene89702.t1 |
| chr4.4 | CDS | 5041650 | 5042093 | 5041650 | ID=cds.MS.gene89702.t1;Parent=MS.gene89702.t1 |
| Gene Sequence |
| Protein sequence |
