Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90151.t1 | XP_003596509.1 | 98.2 | 110 | 2 | 0 | 1 | 110 | 1 | 110 | 1.20E-56 | 228.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90151.t1 | Q9LSQ6 | 56.9 | 102 | 42 | 2 | 2 | 101 | 16 | 117 | 3.3e-26 | 119.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90151.t1 | Q2HVQ3 | 98.2 | 110 | 2 | 0 | 1 | 110 | 1 | 110 | 8.9e-57 | 228.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene90151 | 0.82505 | 5.79E-54 | -1.69E-46 |
| MS.gene049501 | MS.gene90151 | 0.806085 | 9.76E-50 | -1.69E-46 |
| MS.gene050020 | MS.gene90151 | 0.80189 | 7.27E-49 | -1.69E-46 |
| MS.gene050438 | MS.gene90151 | 0.806107 | 9.66E-50 | -1.69E-46 |
| MS.gene050586 | MS.gene90151 | 0.805427 | 1.34E-49 | -1.69E-46 |
| MS.gene050807 | MS.gene90151 | 0.810437 | 1.15E-50 | -1.69E-46 |
| MS.gene050815 | MS.gene90151 | 0.844242 | 8.54E-59 | -1.69E-46 |
| MS.gene051180 | MS.gene90151 | 0.800803 | 1.21E-48 | -1.69E-46 |
| MS.gene051328 | MS.gene90151 | 0.805838 | 1.10E-49 | -1.69E-46 |
| MS.gene051626 | MS.gene90151 | 0.802532 | 5.37E-49 | -1.69E-46 |
| MS.gene052020 | MS.gene90151 | 0.800479 | 1.41E-48 | -1.69E-46 |
| MS.gene052665 | MS.gene90151 | 0.821505 | 3.90E-53 | -1.69E-46 |
| MS.gene052896 | MS.gene90151 | 0.816068 | 6.69E-52 | -1.69E-46 |
| MS.gene053008 | MS.gene90151 | 0.817589 | 3.05E-52 | -1.69E-46 |
| MS.gene053074 | MS.gene90151 | 0.843562 | 1.30E-58 | -1.69E-46 |
| MS.gene054184 | MS.gene90151 | 0.841202 | 5.48E-58 | -1.69E-46 |
| MS.gene054185 | MS.gene90151 | 0.844592 | 6.87E-59 | -1.69E-46 |
| MS.gene054713 | MS.gene90151 | 0.812977 | 3.23E-51 | -1.69E-46 |
| MS.gene055608 | MS.gene90151 | 0.832705 | 8.12E-56 | -1.69E-46 |
| MS.gene055773 | MS.gene90151 | 0.805772 | 1.14E-49 | -1.69E-46 |
| MS.gene055860 | MS.gene90151 | 0.801714 | 7.90E-49 | -1.69E-46 |
| MS.gene056552 | MS.gene90151 | 0.800491 | 1.41E-48 | -1.69E-46 |
| MS.gene057140 | MS.gene90151 | 0.801297 | 9.62E-49 | -1.69E-46 |
| MS.gene058168 | MS.gene90151 | 0.856152 | 3.90E-62 | -1.69E-46 |
| MS.gene058906 | MS.gene90151 | 0.814547 | 1.46E-51 | -1.69E-46 |
| MS.gene059006 | MS.gene90151 | 0.804043 | 2.61E-49 | -1.69E-46 |
| MS.gene059124 | MS.gene90151 | 0.808882 | 2.49E-50 | -1.69E-46 |
| MS.gene059421 | MS.gene90151 | 0.80004 | 1.74E-48 | -1.69E-46 |
| MS.gene059937 | MS.gene90151 | 0.818473 | 1.93E-52 | -1.69E-46 |
| MS.gene060802 | MS.gene90151 | 0.802259 | 6.11E-49 | -1.69E-46 |
| MS.gene060803 | MS.gene90151 | 0.810414 | 1.17E-50 | -1.69E-46 |
| MS.gene060998 | MS.gene90151 | 0.82497 | 6.05E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90151.t1 | MTR_2g081300 | 98.182 | 110 | 2 | 0 | 1 | 110 | 1 | 110 | 2.77e-74 | 215 |
| MS.gene90151.t1 | MTR_2g081350 | 89.815 | 108 | 11 | 0 | 3 | 110 | 7 | 114 | 3.49e-67 | 197 |
| MS.gene90151.t1 | MTR_2g081440 | 84.404 | 109 | 17 | 0 | 2 | 110 | 6 | 114 | 5.22e-64 | 189 |
| MS.gene90151.t1 | MTR_8g466250 | 67.708 | 96 | 30 | 1 | 5 | 99 | 11 | 106 | 4.87e-39 | 126 |
| MS.gene90151.t1 | MTR_2g081580 | 62.626 | 99 | 36 | 1 | 2 | 99 | 7 | 105 | 2.78e-38 | 125 |
| MS.gene90151.t1 | MTR_2g011400 | 65.957 | 94 | 31 | 1 | 5 | 97 | 14 | 107 | 5.54e-38 | 124 |
| MS.gene90151.t1 | MTR_2g089210 | 60.215 | 93 | 36 | 1 | 3 | 94 | 8 | 100 | 6.22e-35 | 116 |
| MS.gene90151.t1 | MTR_1g052885 | 62.637 | 91 | 33 | 1 | 5 | 94 | 6 | 96 | 2.21e-33 | 112 |
| MS.gene90151.t1 | MTR_8g027180 | 54.737 | 95 | 42 | 1 | 1 | 94 | 15 | 109 | 5.87e-29 | 101 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90151.t1 | AT5G54490 | 56.311 | 103 | 43 | 2 | 1 | 101 | 15 | 117 | 5.26e-34 | 114 |
| MS.gene90151.t1 | AT4G27280 | 59.000 | 100 | 39 | 2 | 4 | 101 | 18 | 117 | 1.18e-32 | 111 |
| MS.gene90151.t1 | AT2G46600 | 52.632 | 95 | 44 | 1 | 1 | 94 | 18 | 112 | 8.01e-28 | 99.0 |
Find 27 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATGGATTTCGTTTGCTTA+TGG | 0.265917 | 2.3:+17097983 | MS.gene90151:CDS |
| TGACGAGTTGTGCATAATTT+AGG | 0.279213 | 2.3:-17098191 | None:intergenic |
| TTAGGAGCAGAAGGGTTTAT+AGG | 0.312299 | 2.3:+17097951 | MS.gene90151:CDS |
| CCATCCATGATTTCACGTTT+AGG | 0.332920 | 2.3:+17097933 | MS.gene90151:CDS |
| CATGCTAATGGAAGGTGATT+TGG | 0.335601 | 2.3:+17098094 | MS.gene90151:CDS |
| AAGATGAATTGTTTCATGCT+TGG | 0.428476 | 2.3:+17098041 | MS.gene90151:CDS |
| TTGTTTCATGCTTGGCTTGG+AGG | 0.430866 | 2.3:+17098049 | MS.gene90151:CDS |
| TAGGTTGAGTCCATGTTTAA+TGG | 0.441618 | 2.3:+17098163 | MS.gene90151:CDS |
| TGCTCCTAAACGTGAAATCA+TGG | 0.448752 | 2.3:-17097937 | None:intergenic |
| AGATGGTGCTTTAAATCAAA+TGG | 0.452147 | 2.3:+17098121 | MS.gene90151:CDS |
| AGAGCTCGTGTACATGCTAA+TGG | 0.465464 | 2.3:+17098082 | MS.gene90151:CDS |
| CATGCTTGGCTTGGAGGTGA+GGG | 0.472194 | 2.3:+17098055 | MS.gene90151:CDS |
| GAATTGTTTCATGCTTGGCT+TGG | 0.505364 | 2.3:+17098046 | MS.gene90151:CDS |
| TTTATAGGTGAACTTTGCAA+TGG | 0.524224 | 2.3:+17097966 | MS.gene90151:CDS |
| TTGAGTCCATGTTTAATGGA+TGG | 0.531601 | 2.3:+17098167 | MS.gene90151:CDS |
| TCATGCTTGGCTTGGAGGTG+AGG | 0.539439 | 2.3:+17098054 | MS.gene90151:CDS |
| ATTTCACGTTTAGGAGCAGA+AGG | 0.552251 | 2.3:+17097942 | MS.gene90151:CDS |
| GAGTTGTATTACATTAACAT+TGG | 0.578771 | 2.3:-17098224 | None:intergenic |
| CCTAAACGTGAAATCATGGA+TGG | 0.586937 | 2.3:-17097933 | None:intergenic |
| ATGCACAACTCGTCAAGGCG+CGG | 0.603525 | 2.3:+17098199 | MS.gene90151:CDS |
| AAATTATGCACAACTCGTCA+AGG | 0.609447 | 2.3:+17098194 | MS.gene90151:CDS |
| CTCGTGTACATGCTAATGGA+AGG | 0.614676 | 2.3:+17098086 | MS.gene90151:CDS |
| TTAGGTCCATCCATTAAACA+TGG | 0.615069 | 2.3:-17098173 | None:intergenic |
| GAAGGTGATTTGGATGGAGA+TGG | 0.627275 | 2.3:+17098104 | MS.gene90151:CDS |
| TTGCTTATGGATGTGAACAA+AGG | 0.641088 | 2.3:+17097996 | MS.gene90151:CDS |
| CTAATGGAAGGTGATTTGGA+TGG | 0.674005 | 2.3:+17098098 | MS.gene90151:CDS |
| TTTCACGTTTAGGAGCAGAA+GGG | 0.687848 | 2.3:+17097943 | MS.gene90151:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGATGAATTGTTTCATGCT+TGG | + | chr2.3:17098041-17098060 | MS.gene90151:CDS | 30.0% | |
| TTTATAGGTGAACTTTGCAA+TGG | + | chr2.3:17097966-17097985 | MS.gene90151:CDS | 30.0% | |
| ! | AGATGGTGCTTTAAATCAAA+TGG | + | chr2.3:17098121-17098140 | MS.gene90151:CDS | 30.0% |
| ! | AGTTTTGCATTCTCATGTTT+AGG | + | chr2.3:17098144-17098163 | MS.gene90151:CDS | 30.0% |
| AAATTATGCACAACTCGTCA+AGG | + | chr2.3:17098194-17098213 | MS.gene90151:CDS | 35.0% | |
| TGACGAGTTGTGCATAATTT+AGG | - | chr2.3:17098194-17098213 | None:intergenic | 35.0% | |
| TTAGGTCCATCCATTAAACA+TGG | - | chr2.3:17098176-17098195 | None:intergenic | 35.0% | |
| TTGAGTCCATGTTTAATGGA+TGG | + | chr2.3:17098167-17098186 | MS.gene90151:CDS | 35.0% | |
| TTGCTTATGGATGTGAACAA+AGG | + | chr2.3:17097996-17098015 | MS.gene90151:CDS | 35.0% | |
| ! | CAATGGATTTCGTTTGCTTA+TGG | + | chr2.3:17097983-17098002 | MS.gene90151:CDS | 35.0% |
| ! | TAGGTTGAGTCCATGTTTAA+TGG | + | chr2.3:17098163-17098182 | MS.gene90151:CDS | 35.0% |
| ATTTCACGTTTAGGAGCAGA+AGG | + | chr2.3:17097942-17097961 | MS.gene90151:CDS | 40.0% | |
| CATGCTAATGGAAGGTGATT+TGG | + | chr2.3:17098094-17098113 | MS.gene90151:CDS | 40.0% | |
| CCATCCATGATTTCACGTTT+AGG | + | chr2.3:17097933-17097952 | MS.gene90151:CDS | 40.0% | |
| CCTAAACGTGAAATCATGGA+TGG | - | chr2.3:17097936-17097955 | None:intergenic | 40.0% | |
| GAATTGTTTCATGCTTGGCT+TGG | + | chr2.3:17098046-17098065 | MS.gene90151:CDS | 40.0% | |
| TGCTCCTAAACGTGAAATCA+TGG | - | chr2.3:17097940-17097959 | None:intergenic | 40.0% | |
| TTAGGAGCAGAAGGGTTTAT+AGG | + | chr2.3:17097951-17097970 | MS.gene90151:CDS | 40.0% | |
| TTTCACGTTTAGGAGCAGAA+GGG | + | chr2.3:17097943-17097962 | MS.gene90151:CDS | 40.0% | |
| ! | CTAATGGAAGGTGATTTGGA+TGG | + | chr2.3:17098098-17098117 | MS.gene90151:CDS | 40.0% |
| AGAGCTCGTGTACATGCTAA+TGG | + | chr2.3:17098082-17098101 | MS.gene90151:CDS | 45.0% | |
| CTCGTGTACATGCTAATGGA+AGG | + | chr2.3:17098086-17098105 | MS.gene90151:CDS | 45.0% | |
| ! | GAAGGTGATTTGGATGGAGA+TGG | + | chr2.3:17098104-17098123 | MS.gene90151:CDS | 45.0% |
| !! | TTGTTTCATGCTTGGCTTGG+AGG | + | chr2.3:17098049-17098068 | MS.gene90151:CDS | 45.0% |
| ATGCACAACTCGTCAAGGCG+CGG | + | chr2.3:17098199-17098218 | MS.gene90151:CDS | 55.0% | |
| !! | CATGCTTGGCTTGGAGGTGA+GGG | + | chr2.3:17098055-17098074 | MS.gene90151:CDS | 55.0% |
| !! | TCATGCTTGGCTTGGAGGTG+AGG | + | chr2.3:17098054-17098073 | MS.gene90151:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 17097906 | 17098238 | 17097906 | ID=MS.gene90151 |
| chr2.3 | mRNA | 17097906 | 17098238 | 17097906 | ID=MS.gene90151.t1;Parent=MS.gene90151 |
| chr2.3 | exon | 17097906 | 17098238 | 17097906 | ID=MS.gene90151.t1.exon1;Parent=MS.gene90151.t1 |
| chr2.3 | CDS | 17097906 | 17098238 | 17097906 | ID=cds.MS.gene90151.t1;Parent=MS.gene90151.t1 |
| Gene Sequence |
| Protein sequence |
