AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92313


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92313.t1 MTR_5g005960 90.323 372 15 8 1 370 1 353 1.67e-174 489
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92313.t1 AT5G26230 46.970 330 134 8 44 355 25 331 8.17e-71 225

Find 78 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTACTAACCCCTGGTTTAT+TGG 0.157769 5.1:+914885 None:intergenic
GGAGGAAGTGGCGGAGGAGG+TGG 0.198247 5.1:+915674 None:intergenic
GAAACACAGTTTCTCTTTCT+TGG 0.237235 5.1:+915066 None:intergenic
GGAAGTGGCGGAGGAGGTGG+AGG 0.258777 5.1:+915677 None:intergenic
AGTGGCGGAGGAGGTGGAGG+AGG 0.348526 5.1:+915680 None:intergenic
AGATCTTCACCAAGTCATTC+TGG 0.364909 5.1:-915024 MS.gene92313:CDS
TAGCAACAGTTTCACCTTCT+CGG 0.380074 5.1:+915367 None:intergenic
GGAGGAGGAAGTGGCGGAGG+AGG 0.382522 5.1:+915671 None:intergenic
TTCTGGCGTTCTTTCACAAA+AGG 0.390582 5.1:-915007 MS.gene92313:CDS
AGCAACAGTTTCACCTTCTC+GGG 0.399484 5.1:+915368 None:intergenic
CAAAAGGGTGTTCTTCTTCG+TGG 0.404135 5.1:-914991 MS.gene92313:CDS
AGAGAAGAAAAGAGCTGAGA+TGG 0.412803 5.1:-915937 MS.gene92313:CDS
CGAGAAGAATCACAAGAATC+AGG 0.418416 5.1:+915570 None:intergenic
GAGAAGAAAAGAGCTGAGAT+GGG 0.425520 5.1:-915936 MS.gene92313:CDS
GAAAGAAGTGGTGGAATCAT+TGG 0.430173 5.1:+915614 None:intergenic
AGACAAAGAGAAATACTTGT+TGG 0.431020 5.1:+915452 None:intergenic
CTTCTCTACCACATCATACA+TGG 0.432454 5.1:+915953 None:intergenic
GGAGGATTTGCGAGGTGAAA+TGG 0.432667 5.1:+915803 None:intergenic
AGCAGTGGAGGAGGAAGTGG+CGG 0.433040 5.1:+915665 None:intergenic
AGTGGAGGAGGAAGTGGCGG+AGG 0.439902 5.1:+915668 None:intergenic
GGGAGCAGTGGAGGAGGAAG+TGG 0.441095 5.1:+915662 None:intergenic
AAAGAAACCGTTGCTAAAAC+AGG 0.445355 5.1:-915408 MS.gene92313:CDS
TCTTCTTCTGTTACAGAACT+TGG 0.446376 5.1:+915546 None:intergenic
CGCTACTACCGGTGGAATCA+CGG 0.456800 5.1:+915640 None:intergenic
TGAATTGGATCTAGTGTTGT+TGG 0.465264 5.1:+915488 None:intergenic
ATCATTGGAGGCGCTACTAC+CGG 0.488441 5.1:+915629 None:intergenic
AATGAGTGTTACTGCTAAAG+AGG 0.495711 5.1:-915310 MS.gene92313:CDS
ATGAGTAATAGCACCTTGAA+TGG 0.497731 5.1:+914927 None:intergenic
TGTGAAAGTTTCTCGTACAA+TGG 0.500772 5.1:+915258 None:intergenic
GGAATCACGGGGAGCAGTGG+AGG 0.500991 5.1:+915653 None:intergenic
AGGTGGAGGAGGAAGAGAGA+AGG 0.508253 5.1:+915691 None:intergenic
GGCACTCTGCAACTCTTCCA+TGG 0.508446 5.1:+914948 None:intergenic
GCTACTACCGGTGGAATCAC+GGG 0.509033 5.1:+915641 None:intergenic
TCTGGCGTTCTTTCACAAAA+GGG 0.511252 5.1:-915006 MS.gene92313:CDS
ATACGAGGGGAGAGATGAAG+AGG 0.517544 5.1:+915732 None:intergenic
GGTGGAATCACGGGGAGCAG+TGG 0.517781 5.1:+915650 None:intergenic
GCAGATGAACTCTTCTACAA+AGG 0.517915 5.1:-915765 MS.gene92313:CDS
ATCACGGGGAGCAGTGGAGG+AGG 0.520130 5.1:+915656 None:intergenic
AATGAGTGTGATGGTGATGA+AGG 0.520154 5.1:+915864 None:intergenic
GGTGAAGATCTCATCGAAGA+AGG 0.550102 5.1:+915036 None:intergenic
TCATGATTACTACTAACCCC+TGG 0.551114 5.1:+914877 None:intergenic
TGCAGGACAGAGAGTGTTGG+AGG 0.552177 5.1:+915785 None:intergenic
ACTGCTCCCCGTGATTCCAC+CGG 0.554614 5.1:-915648 MS.gene92313:CDS
ACAGCAACAAACAGTGGAGG+AGG 0.558053 5.1:-915232 MS.gene92313:CDS
GAGAAGGAGAGTACGTACCA+TGG 0.559429 5.1:+915707 None:intergenic
GAGTTGCAGAGTGCCATTCA+AGG 0.563158 5.1:-914940 MS.gene92313:CDS
AGAGATGAAGAGGTAAGAGT+TGG 0.563757 5.1:+915742 None:intergenic
AACGTTAACTACTACGACAA+CGG 0.575052 5.1:-915184 MS.gene92313:CDS
TGATCGAGATGCTTCTTCCA+TGG 0.576373 5.1:-914965 MS.gene92313:CDS
AGAAGAAAAGAGCTGAGATG+GGG 0.580424 5.1:-915935 MS.gene92313:CDS
TTGTAGAAGAGTTCATCTGC+AGG 0.581095 5.1:+915768 None:intergenic
ATCTGCAGGACAGAGAGTGT+TGG 0.581581 5.1:+915782 None:intergenic
TCTTTGATTACCAATAAACC+AGG 0.582297 5.1:-914895 MS.gene92313:CDS
TCTCTCCCCTCGTATCTCCA+TGG 0.583262 5.1:-915724 MS.gene92313:CDS
ATCACTGATGAAAGAAGTGG+TGG 0.586109 5.1:+915605 None:intergenic
GAGAAGAAAGAGAATGTGGC+GGG 0.590250 5.1:-915129 MS.gene92313:CDS
ACCGTTGCTAAAACCCGAGA+AGG 0.590587 5.1:-915381 MS.gene92313:CDS
ATCTGAGAAGAAAGAGAATG+TGG 0.594317 5.1:-915133 MS.gene92313:CDS
AGAAGTGGTGGAATCATTGG+AGG 0.594966 5.1:+915617 None:intergenic
CTTTGATTACCAATAAACCA+GGG 0.602225 5.1:-914894 MS.gene92313:CDS
ATTGGATCTAGTGTTGTTGG+AGG 0.616982 5.1:+915491 None:intergenic
GAAAGAACGCCAGAATGACT+TGG 0.620408 5.1:+915015 None:intergenic
GATTGTGAACTCAAAATCTG+AGG 0.622599 5.1:+915827 None:intergenic
ATTGGAGGCGCTACTACCGG+TGG 0.625649 5.1:+915632 None:intergenic
ATGAGTGTGATGGTGATGAA+GGG 0.631861 5.1:+915865 None:intergenic
TACGTACCATGGAGATACGA+GGG 0.636974 5.1:+915718 None:intergenic
AAGGGTGTTCTTCTTCGTGG+TGG 0.640900 5.1:-914988 MS.gene92313:CDS
AAGATCACTGATGAAAGAAG+TGG 0.640945 5.1:+915602 None:intergenic
CAGCAACAAACAGTGGAGGA+GGG 0.645100 5.1:-915231 MS.gene92313:CDS
AAAACAGCAACAAACAGTGG+AGG 0.646252 5.1:-915235 MS.gene92313:CDS
TGAGAAGAAAGAGAATGTGG+CGG 0.646818 5.1:-915130 MS.gene92313:CDS
CTACTACCGGTGGAATCACG+GGG 0.647123 5.1:+915642 None:intergenic
GATGATGAGAATGAGTGTGA+TGG 0.651954 5.1:+915855 None:intergenic
TTTGATTACCAATAAACCAG+GGG 0.666066 5.1:-914893 MS.gene92313:CDS
GTACGTACCATGGAGATACG+AGG 0.687365 5.1:+915717 None:intergenic
AGAGTGTTGGAGGATTTGCG+AGG 0.696367 5.1:+915795 None:intergenic
ACGTACCATGGAGATACGAG+GGG 0.713565 5.1:+915719 None:intergenic
GGACGCAACCATGTATGATG+TGG 0.730640 5.1:-915961 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGTTATCTCATTCGTTTT+CGG - chr5.1:915709-915728 MS.gene92313:CDS 25.0%
AGACAAAGAGAAATACTTGT+TGG + chr5.1:915363-915382 None:intergenic 30.0%
CTTTGATTACCAATAAACCA+GGG - chr5.1:915918-915937 MS.gene92313:CDS 30.0%
TCTTTGATTACCAATAAACC+AGG - chr5.1:915917-915936 MS.gene92313:CDS 30.0%
TTTGATTACCAATAAACCAG+GGG - chr5.1:915919-915938 MS.gene92313:CDS 30.0%
! ATGTTATCTCATTCGTTTTC+GGG - chr5.1:915710-915729 MS.gene92313:CDS 30.0%
! TGTTTCTTTTGCTCTGTTTT+TGG + chr5.1:914913-914932 None:intergenic 30.0%
AAAGAAACCGTTGCTAAAAC+AGG - chr5.1:915404-915423 MS.gene92313:CDS 35.0%
AACGTTAACTACTACGACAA+CGG - chr5.1:915628-915647 MS.gene92313:CDS 35.0%
AAGATCACTGATGAAAGAAG+TGG + chr5.1:915213-915232 None:intergenic 35.0%
ACAAAAACAGCAACAAACAG+TGG - chr5.1:915574-915593 MS.gene92313:CDS 35.0%
ATCTGAGAAGAAAGAGAATG+TGG - chr5.1:915679-915698 MS.gene92313:CDS 35.0%
ATGAGTAATAGCACCTTGAA+TGG + chr5.1:915888-915907 None:intergenic 35.0%
GAAACACAGTTTCTCTTTCT+TGG + chr5.1:915749-915768 None:intergenic 35.0%
GATTGTGAACTCAAAATCTG+AGG + chr5.1:914988-915007 None:intergenic 35.0%
GGTTTCTTTACGAAAAACAG+AGG + chr5.1:915393-915412 None:intergenic 35.0%
TCTTCTTCTGTTACAGAACT+TGG + chr5.1:915269-915288 None:intergenic 35.0%
TGAATTGGATCTAGTGTTGT+TGG + chr5.1:915327-915346 None:intergenic 35.0%
TGTGAAAGTTTCTCGTACAA+TGG + chr5.1:915557-915576 None:intergenic 35.0%
! AATGAGTGTTACTGCTAAAG+AGG - chr5.1:915502-915521 MS.gene92313:CDS 35.0%
! GGTTTTCTTCAACTGTGAAT+TGG + chr5.1:915342-915361 None:intergenic 35.0%
! TGTTATCTCATTCGTTTTCG+GGG - chr5.1:915711-915730 MS.gene92313:CDS 35.0%
!! ATTCGTTTTCGGGGAATTTA+AGG - chr5.1:915720-915739 MS.gene92313:CDS 35.0%
AAAACAGCAACAAACAGTGG+AGG - chr5.1:915577-915596 MS.gene92313:CDS 40.0%
ACTACTAACCCCTGGTTTAT+TGG + chr5.1:915930-915949 None:intergenic 40.0%
AGAAGAAAAGAGCTGAGATG+GGG - chr5.1:914877-914896 MS.gene92313:CDS 40.0%
AGAGAAGAAAAGAGCTGAGA+TGG - chr5.1:914875-914894 MS.gene92313:CDS 40.0%
AGAGATGAAGAGGTAAGAGT+TGG + chr5.1:915073-915092 None:intergenic 40.0%
AGATCTTCACCAAGTCATTC+TGG - chr5.1:915788-915807 MS.gene92313:CDS 40.0%
ATCACTGATGAAAGAAGTGG+TGG + chr5.1:915210-915229 None:intergenic 40.0%
CGAGAAGAATCACAAGAATC+AGG + chr5.1:915245-915264 None:intergenic 40.0%
GAGAAGAAAAGAGCTGAGAT+GGG - chr5.1:914876-914895 MS.gene92313:CDS 40.0%
GATGATGAGAATGAGTGTGA+TGG + chr5.1:914960-914979 None:intergenic 40.0%
GCAGATGAACTCTTCTACAA+AGG - chr5.1:915047-915066 MS.gene92313:CDS 40.0%
TAGCAACAGTTTCACCTTCT+CGG + chr5.1:915448-915467 None:intergenic 40.0%
TCATGATTACTACTAACCCC+TGG + chr5.1:915938-915957 None:intergenic 40.0%
TCTGGCGTTCTTTCACAAAA+GGG - chr5.1:915806-915825 MS.gene92313:CDS 40.0%
TGAGAAGAAAGAGAATGTGG+CGG - chr5.1:915682-915701 MS.gene92313:CDS 40.0%
TTCTGGCGTTCTTTCACAAA+AGG - chr5.1:915805-915824 MS.gene92313:CDS 40.0%
TTGTAGAAGAGTTCATCTGC+AGG + chr5.1:915047-915066 None:intergenic 40.0%
! AATGAGTGTGATGGTGATGA+AGG + chr5.1:914951-914970 None:intergenic 40.0%
! ATGAGTGTGATGGTGATGAA+GGG + chr5.1:914950-914969 None:intergenic 40.0%
! ATTGGATCTAGTGTTGTTGG+AGG + chr5.1:915324-915343 None:intergenic 40.0%
! GAAAGAAGTGGTGGAATCAT+TGG + chr5.1:915201-915220 None:intergenic 40.0%
!!! GGTTTCACCTGTTTTAGCAA+CGG + chr5.1:915414-915433 None:intergenic 40.0%
AGCAACAGTTTCACCTTCTC+GGG + chr5.1:915447-915466 None:intergenic 45.0%
GAAAGAACGCCAGAATGACT+TGG + chr5.1:915800-915819 None:intergenic 45.0%
GAGAAGAAAGAGAATGTGGC+GGG - chr5.1:915683-915702 MS.gene92313:CDS 45.0%
GGTGAAGATCTCATCGAAGA+AGG + chr5.1:915779-915798 None:intergenic 45.0%
TACGTACCATGGAGATACGA+GGG + chr5.1:915097-915116 None:intergenic 45.0%
TGATCGAGATGCTTCTTCCA+TGG - chr5.1:915847-915866 MS.gene92313:CDS 45.0%
! AGAAGTGGTGGAATCATTGG+AGG + chr5.1:915198-915217 None:intergenic 45.0%
!! ACCTTCTCGGGTTTTAGCAA+CGG + chr5.1:915435-915454 None:intergenic 45.0%
!! CAAAAGGGTGTTCTTCTTCG+TGG - chr5.1:915821-915840 MS.gene92313:CDS 45.0%
ACAGCAACAAACAGTGGAGG+AGG - chr5.1:915580-915599 MS.gene92313:CDS 50.0%
ACCGTTGCTAAAACCCGAGA+AGG - chr5.1:915431-915450 MS.gene92313:CDS 50.0%
ACGTACCATGGAGATACGAG+GGG + chr5.1:915096-915115 None:intergenic 50.0%
ATACGAGGGGAGAGATGAAG+AGG + chr5.1:915083-915102 None:intergenic 50.0%
CAGCAACAAACAGTGGAGGA+GGG - chr5.1:915581-915600 MS.gene92313:CDS 50.0%
GGAGGATTTGCGAGGTGAAA+TGG + chr5.1:915012-915031 None:intergenic 50.0%
GTACGTACCATGGAGATACG+AGG + chr5.1:915098-915117 None:intergenic 50.0%
! ATCTGCAGGACAGAGAGTGT+TGG + chr5.1:915033-915052 None:intergenic 50.0%
! GAGAAGGAGAGTACGTACCA+TGG + chr5.1:915108-915127 None:intergenic 50.0%
! GAGTTGCAGAGTGCCATTCA+AGG - chr5.1:915872-915891 MS.gene92313:CDS 50.0%
!! AAGGGTGTTCTTCTTCGTGG+TGG - chr5.1:915824-915843 MS.gene92313:CDS 50.0%
!! AGAGTGTTGGAGGATTTGCG+AGG + chr5.1:915020-915039 None:intergenic 50.0%
!! ATCATTGGAGGCGCTACTAC+CGG + chr5.1:915186-915205 None:intergenic 50.0%
AGGTGGAGGAGGAAGAGAGA+AGG + chr5.1:915124-915143 None:intergenic 55.0%
CGCTACTACCGGTGGAATCA+CGG + chr5.1:915175-915194 None:intergenic 55.0%
CTACTACCGGTGGAATCACG+GGG + chr5.1:915173-915192 None:intergenic 55.0%
GCTACTACCGGTGGAATCAC+GGG + chr5.1:915174-915193 None:intergenic 55.0%
GGCACTCTGCAACTCTTCCA+TGG + chr5.1:915867-915886 None:intergenic 55.0%
TCTCTCCCCTCGTATCTCCA+TGG - chr5.1:915088-915107 MS.gene92313:CDS 55.0%
!! TGCAGGACAGAGAGTGTTGG+AGG + chr5.1:915030-915049 None:intergenic 55.0%
ACTGCTCCCCGTGATTCCAC+CGG - chr5.1:915164-915183 MS.gene92313:CDS 60.0%
AGCAGTGGAGGAGGAAGTGG+CGG + chr5.1:915150-915169 None:intergenic 60.0%
!! ATTGGAGGCGCTACTACCGG+TGG + chr5.1:915183-915202 None:intergenic 60.0%
AGTGGAGGAGGAAGTGGCGG+AGG + chr5.1:915147-915166 None:intergenic 65.0%
ATCACGGGGAGCAGTGGAGG+AGG + chr5.1:915159-915178 None:intergenic 65.0%
GGAATCACGGGGAGCAGTGG+AGG + chr5.1:915162-915181 None:intergenic 65.0%
GGGAGCAGTGGAGGAGGAAG+TGG + chr5.1:915153-915172 None:intergenic 65.0%
GGTGGAATCACGGGGAGCAG+TGG + chr5.1:915165-915184 None:intergenic 65.0%
! AGTGGCGGAGGAGGTGGAGG+AGG + chr5.1:915135-915154 None:intergenic 70.0%
! GGAAGTGGCGGAGGAGGTGG+AGG + chr5.1:915138-915157 None:intergenic 70.0%
! GGAGGAAGTGGCGGAGGAGG+TGG + chr5.1:915141-915160 None:intergenic 70.0%
! GGAGGAGGAAGTGGCGGAGG+AGG + chr5.1:915144-915163 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 914861 915973 914861 ID=MS.gene92313
chr5.1 mRNA 914861 915973 914861 ID=MS.gene92313.t1;Parent=MS.gene92313
chr5.1 exon 914861 915973 914861 ID=MS.gene92313.t1.exon1;Parent=MS.gene92313.t1
chr5.1 CDS 914861 915973 914861 ID=cds.MS.gene92313.t1;Parent=MS.gene92313.t1
Gene Sequence

>MS.gene92313

ATGTATGATGTGGTAGAGAAGAAAAGAGCTGAGATGGGGAAGAAAAGAACCAAAAACAGAGCAAAAGAAACAAAATCATACACACTCCCTTCATCACCATCACACTCATTCTCATCATCTTCTTCCTCAGATTTTGAGTTCACAATCTCCATTTCACCTCGCAAATCCTCCAACACTCTCTGTCCTGCAGATGAACTCTTCTACAAAGGCCAACTCTTACCTCTTCATCTCTCCCCTCGTATCTCCATGGTACGTACTCTCCTTCTCTCTTCCTCCTCCACCTCCTCCGCCACTTCCTCCTCCACTGCTCCCCGTGATTCCACCGGTAGTAGCGCCTCCAATGATTCCACCACTTCTTTCATCAGTGATCTTGCTCTTTTTCCTGATTCTTGTGATTCTTCTCGACCAAGTTCTGTAACAGAAGAAGATGAGTTAAAACGTCTTCATAATCACAACAATTCCTCCAACAACACTAGATCCAATTCACAGTTGAAGAAAACCAACAAGTATTTCTCTTTGTCTAGATTCTCCTCTGTTTTTCGTAAAGAAACCGTTGCTAAAACAGGTGAAACCGTTGCTAAAACCCGAGAAGGTGAAACTGTTGCTAATAGTATTAACAACAGTTCAGTGAAGATGAAGAGAATGAGTGTTACTGCTAAAGAGGTGATACGAAAATATTTCAAGAAAGTGAAACCATTGTACGAGAAACTTTCACAAAAACAGCAACAAACAGTGGAGGAGGGTGCAAATAGCAATACAAATACAACAACGTTAACTACTACGACAACGGTTATGTCTTTGTTAACAAAAACAGAGAGATCTGAGAAGAAAGAGAATGTGGCGGGTACTATGTTATCTCATTCGTTTTCGGGGAATTTAAGGTATCCAAGAAAGAGAAACTGTGTTTCAAGTTGTCCTTCTTCGATGAGATCTTCACCAAGTCATTCTGGCGTTCTTTCACAAAAGGGTGTTCTTCTTCGTGGTGGTGATCGAGATGCTTCTTCCATGGAAGAGTTGCAGAGTGCCATTCAAGGTGCTATTACTCATTGCAAAAACTCTTTGATTACCAATAAACCAGGGGTTAGTAGTAATCATGAAATTAATCTCATCTAA

Protein sequence

>MS.gene92313.t1

MYDVVEKKRAEMGKKRTKNRAKETKSYTLPSSPSHSFSSSSSSDFEFTISISPRKSSNTLCPADELFYKGQLLPLHLSPRISMVRTLLLSSSSTSSATSSSTAPRDSTGSSASNDSTTSFISDLALFPDSCDSSRPSSVTEEDELKRLHNHNNSSNNTRSNSQLKKTNKYFSLSRFSSVFRKETVAKTGETVAKTREGETVANSINNSSVKMKRMSVTAKEVIRKYFKKVKPLYEKLSQKQQQTVEEGANSNTNTTTLTTTTTVMSLLTKTERSEKKENVAGTMLSHSFSGNLRYPRKRNCVSSCPSSMRSSPSHSGVLSQKGVLLRGGDRDASSMEELQSAIQGAITHCKNSLITNKPGVSSNHEINLI