AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99166

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99166.t1 MTR_8g007300 96.364 220 8 0 1 220 1 220 4.60e-155 429
MS.gene99166.t1 MTR_3g096330 59.804 204 81 1 9 212 560 762 1.62e-78 250
MS.gene99166.t1 MTR_5g036570 45.304 181 94 4 24 201 25 203 5.87e-41 139
MS.gene99166.t1 MTR_1g085500 42.632 190 102 3 25 211 22 207 1.46e-37 130
MS.gene99166.t1 MTR_5g046410 38.532 218 118 5 11 214 4 219 6.92e-37 129
MS.gene99166.t1 MTR_5g046430 38.532 218 118 5 11 214 4 219 6.92e-37 129
MS.gene99166.t1 MTR_4g017030 38.532 218 118 5 11 214 4 219 6.92e-37 129
MS.gene99166.t1 MTR_2g019250 38.991 218 117 5 11 214 4 219 1.38e-36 128
MS.gene99166.t1 MTR_4g017040 40.000 200 107 4 26 214 22 219 2.21e-36 127
MS.gene99166.t1 MTR_4g017050 38.073 218 119 5 11 214 4 219 2.68e-36 127
MS.gene99166.t1 MTR_1g060120 36.453 203 122 2 14 211 14 214 1.35e-35 125
MS.gene99166.t1 MTR_7g111240 44.720 161 86 2 25 184 19 177 2.93e-35 124
MS.gene99166.t1 MTR_1g079490 42.593 162 90 1 26 184 22 183 1.49e-34 122
MS.gene99166.t1 MTR_6g005330 36.667 210 124 5 10 216 5 208 7.05e-34 121
MS.gene99166.t1 MTR_7g111240 44.099 161 84 2 25 184 19 174 8.49e-34 120
MS.gene99166.t1 MTR_6g005360 37.306 193 113 4 26 216 22 208 9.82e-33 118
MS.gene99166.t1 MTR_6g005310 37.306 193 113 4 26 216 22 208 1.19e-32 117
MS.gene99166.t1 MTR_6g005340 37.306 193 113 4 26 216 22 208 5.21e-32 116
MS.gene99166.t1 MTR_1g023770 38.725 204 107 4 17 210 13 208 1.35e-31 115
MS.gene99166.t1 MTR_1g085140 38.462 195 102 4 25 203 23 215 2.20e-31 114
MS.gene99166.t1 MTR_2g030855 44.172 163 83 4 25 184 21 178 6.94e-31 113
MS.gene99166.t1 MTR_1g112200 37.576 165 97 1 20 184 17 175 5.18e-30 110
MS.gene99166.t1 MTR_2g041570 35.593 177 103 3 4 179 3 169 1.47e-29 109
MS.gene99166.t1 MTR_2g041580 35.593 177 103 3 4 179 3 169 1.64e-29 109
MS.gene99166.t1 MTR_6g005380 36.816 201 122 3 10 206 5 204 2.28e-29 109
MS.gene99166.t1 MTR_8g020770 45.522 134 70 2 47 178 38 170 8.17e-29 107
MS.gene99166.t1 MTR_2g045100 32.571 175 109 2 5 179 3 168 1.27e-28 107
MS.gene99166.t1 MTR_2g036120 41.353 133 77 1 47 179 37 168 1.28e-28 107
MS.gene99166.t1 MTR_2g041550 35.294 170 101 2 12 180 9 170 1.81e-28 107
MS.gene99166.t1 MTR_2g031270 39.267 191 101 5 30 210 27 212 5.00e-28 105
MS.gene99166.t1 MTR_3g019650 33.019 212 117 5 5 202 3 203 3.14e-27 103
MS.gene99166.t1 MTR_8g020630 30.332 211 125 3 5 202 3 204 5.19e-27 103
MS.gene99166.t1 MTR_8g020590 36.296 135 85 1 47 180 34 168 9.90e-27 102
MS.gene99166.t1 MTR_5g031460 35.971 139 88 1 41 179 31 168 2.42e-26 101
MS.gene99166.t1 MTR_3g019680 35.593 177 100 4 5 179 3 167 2.74e-26 101
MS.gene99166.t1 MTR_2g019780 31.019 216 117 5 7 202 4 207 6.53e-26 100
MS.gene99166.t1 MTR_8g020650 32.544 169 100 2 47 202 37 204 8.20e-26 100
MS.gene99166.t1 MTR_6g005350 39.011 182 104 3 26 203 22 200 8.61e-26 100
MS.gene99166.t1 MTR_3g019710 39.098 133 80 1 47 179 37 168 1.20e-25 99.4
MS.gene99166.t1 MTR_8g020763 37.313 134 82 2 47 179 17 149 1.46e-25 98.6
MS.gene99166.t1 MTR_3g073170 34.320 169 94 3 47 202 41 205 1.50e-25 99.4
MS.gene99166.t1 MTR_2g030895 42.754 138 75 3 51 184 7 144 2.12e-25 98.2
MS.gene99166.t1 MTR_0284s0010 36.158 177 100 4 5 179 3 168 2.55e-25 98.6
MS.gene99166.t1 MTR_2g030865 42.754 138 75 3 51 184 7 144 3.08e-25 97.4
MS.gene99166.t1 MTR_4g010340 36.257 171 95 6 11 179 6 164 5.30e-25 97.8
MS.gene99166.t1 MTR_7g011340 36.364 165 94 2 47 201 37 200 8.79e-25 97.4
MS.gene99166.t1 MTR_8g020610 33.143 175 108 4 8 181 2 168 1.46e-24 96.7
MS.gene99166.t1 MTR_2g044040 33.908 174 104 3 8 179 4 168 1.75e-24 96.3
MS.gene99166.t1 MTR_8g020750 41.045 134 77 2 47 179 39 171 1.16e-23 94.4
MS.gene99166.t1 MTR_8g020670 30.952 168 102 2 48 202 38 204 1.31e-22 91.7
MS.gene99166.t1 MTR_8g020760 37.594 133 82 1 47 179 39 170 3.00e-22 90.5
MS.gene99166.t1 MTR_2g031300 40.146 137 59 3 51 184 7 123 2.38e-20 84.3
MS.gene99166.t1 MTR_2g086640 39.640 111 64 3 86 194 72 181 2.32e-19 82.8
MS.gene99166.t1 MTR_2g086620 37.615 109 67 1 87 194 74 182 9.48e-19 81.3
MS.gene99166.t1 MTR_2g086630 36.697 109 68 1 87 194 74 182 2.89e-18 80.1
MS.gene99166.t1 MTR_4g052770 37.273 110 68 1 86 194 74 183 8.39e-18 78.6
MS.gene99166.t1 MTR_4g052780 37.273 110 68 1 86 194 74 183 1.37e-17 78.2
MS.gene99166.t1 MTR_4g027415 39.623 106 63 1 48 153 38 142 8.97e-16 71.6
MS.gene99166.t1 MTR_3g019810 33.083 133 82 2 47 179 143 268 6.53e-14 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99166.t1 AT5G61750 53.514 185 78 2 1 184 1 178 9.47e-66 202
MS.gene99166.t1 AT1G10460 43.750 160 86 3 25 180 18 177 8.91e-38 131
MS.gene99166.t1 AT3G04200 43.114 167 90 3 22 184 23 188 2.46e-36 127
MS.gene99166.t1 AT3G62020 44.624 186 88 5 10 187 6 184 5.04e-36 126
MS.gene99166.t1 AT1G09560 46.626 163 84 2 23 184 21 181 5.47e-36 126
MS.gene99166.t1 AT1G02335 41.667 168 85 3 26 187 24 184 1.52e-35 125
MS.gene99166.t1 AT5G38930 43.373 166 91 2 25 187 25 190 3.11e-34 122
MS.gene99166.t1 AT5G38960 40.854 164 93 2 25 184 25 188 1.72e-33 120
MS.gene99166.t1 AT5G38940 38.421 190 107 3 25 204 25 214 2.62e-33 119
MS.gene99166.t1 AT3G05950 39.362 188 105 4 22 205 22 204 3.28e-33 119
MS.gene99166.t1 AT5G38940 38.421 190 107 3 25 204 49 238 3.43e-33 120
MS.gene99166.t1 AT3G05930 35.160 219 125 4 1 212 1 209 2.03e-32 117
MS.gene99166.t1 AT3G04170 40.107 187 102 4 22 205 22 201 3.49e-32 117
MS.gene99166.t1 AT3G10080 36.842 190 115 2 25 211 25 212 6.42e-32 116
MS.gene99166.t1 AT5G38910 35.111 225 126 6 1 217 1 213 7.18e-32 116
MS.gene99166.t1 AT5G26700 38.421 190 105 4 1 187 1 181 1.04e-31 115
MS.gene99166.t1 AT4G14630 40.571 175 100 2 17 187 17 191 2.64e-31 114
MS.gene99166.t1 AT3G10080 36.842 190 115 2 25 211 96 283 2.65e-31 116
MS.gene99166.t1 AT5G39110 36.224 196 114 4 17 205 12 203 3.80e-31 114
MS.gene99166.t1 AT3G62020 45.695 151 70 3 44 187 10 155 4.28e-31 113
MS.gene99166.t1 AT5G39120 35.468 203 121 4 7 205 6 202 6.45e-31 113
MS.gene99166.t1 AT5G39150 37.500 184 107 3 26 205 23 202 8.98e-31 113
MS.gene99166.t1 AT1G72610 41.791 134 77 1 48 180 35 168 3.30e-30 111
MS.gene99166.t1 AT5G39180 39.877 163 94 2 26 184 23 185 4.45e-30 111
MS.gene99166.t1 AT3G04190 41.509 159 88 3 30 184 30 187 8.45e-30 110
MS.gene99166.t1 AT5G20630 34.239 184 110 5 4 186 3 176 1.92e-29 109
MS.gene99166.t1 AT3G04180 38.043 184 105 4 26 205 26 204 3.64e-29 108
MS.gene99166.t1 AT5G39160 35.567 194 120 3 22 211 20 212 7.49e-29 108
MS.gene99166.t1 AT5G39130 36.082 194 119 3 22 211 20 212 9.27e-29 108
MS.gene99166.t1 AT5G39190 35.567 194 120 3 22 211 20 212 1.22e-28 107
MS.gene99166.t1 AT1G18970 37.778 180 105 5 10 188 14 187 2.64e-28 106
MS.gene99166.t1 AT3G04150 37.234 188 113 3 1 184 1 187 2.78e-28 107
MS.gene99166.t1 AT1G18980 39.394 165 94 4 25 188 28 187 1.09e-27 105
MS.gene99166.t1 AT3G04150 41.096 146 82 2 43 184 55 200 5.42e-27 103
MS.gene99166.t1 AT5G39160 35.052 194 117 3 22 211 20 208 3.26e-26 101
MS.gene99166.t1 AT5G39160 32.911 158 103 1 57 211 33 190 4.98e-20 84.7
MS.gene99166.t1 AT5G39190 35.507 138 86 1 77 211 53 190 6.01e-20 84.3
MS.gene99166.t1 AT1G74820 30.516 213 134 5 8 210 16 224 7.23e-18 79.3
MS.gene99166.t1 AT5G38910 28.700 223 106 6 1 217 1 176 4.86e-15 70.9

Find 33 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAATATGGTTTGTTTATT+TGG 0.147969 8.2:-82418117 None:intergenic
TGCTAATGAAACTTGGATTT+TGG 0.152319 8.2:-82418055 None:intergenic
CACCAACTTTGAGAACATTT+TGG 0.167372 8.2:-82418422 None:intergenic
CTTTCTGCTTCTCAAGTTCA+TGG 0.253166 8.2:+82418618 MS.gene99166:CDS
GTCACTGCTGCTGAATTTCC+AGG 0.267510 8.2:+82418249 MS.gene99166:CDS
ATGTAATGGAATAAACCTTT+AGG 0.303470 8.2:-82418459 None:intergenic
TCTTGAGCTGTTACATTAGC+TGG 0.333856 8.2:-82418162 None:intergenic
GGGTAGACCATTGATGAATA+TGG 0.338217 8.2:-82418130 None:intergenic
CAGTAGCTCTTGGATGATAA+TGG 0.340284 8.2:-82418335 None:intergenic
TGTTTATTTGGTGGAATTGA+TGG 0.371662 8.2:-82418105 None:intergenic
TTCCAAAATGTTCTCAAAGT+TGG 0.374296 8.2:+82418420 MS.gene99166:CDS
TGTAATGGAATAAACCTTTA+GGG 0.377741 8.2:-82418458 None:intergenic
CACAATCTGCTAATGAAACT+TGG 0.384262 8.2:-82418062 None:intergenic
TTCCATTACATTCTAAATCA+AGG 0.393287 8.2:+82418471 MS.gene99166:CDS
AACCTTGATTTAGAATGTAA+TGG 0.410800 8.2:-82418473 None:intergenic
AAGATCATTTCAGTAGCTCT+TGG 0.441209 8.2:-82418345 None:intergenic
TTTCCAGGGCTGAACACTCT+TGG 0.460820 8.2:+82418264 MS.gene99166:CDS
AATGTTGTGTCAGAAGTTGT+AGG 0.463329 8.2:-82418558 None:intergenic
AGCAGCAGTGACAAGTTTCA+CGG 0.470392 8.2:-82418238 None:intergenic
AACACTCTTGGTCTCTCGAT+TGG 0.484763 8.2:+82418276 MS.gene99166:CDS
AATATGGTTTGTTTATTTGG+TGG 0.491327 8.2:-82418114 None:intergenic
GGAATAATAGATACTTGTCC+AGG 0.507182 8.2:-82418537 None:intergenic
TACAACTTCTGACACAACAT+TGG 0.513431 8.2:+82418560 MS.gene99166:CDS
AAACAAACCATATTCATCAA+TGG 0.515657 8.2:+82418123 MS.gene99166:CDS
AGAATCAATATCGATAATGA+TGG 0.528033 8.2:+82418300 MS.gene99166:CDS
CAATAATATGAATGGCTAAG+AGG 0.529024 8.2:-82418658 None:intergenic
GTGTACAATAGTCAAAATCC+TGG 0.531942 8.2:+82418519 MS.gene99166:CDS
AGACCAAGAGTGTTCAGCCC+TGG 0.540141 8.2:-82418267 None:intergenic
TCACTGCTGCTGAATTTCCA+GGG 0.546815 8.2:+82418250 MS.gene99166:CDS
AAAGAAAATATTGTAAACAA+AGG 0.628168 8.2:-82418010 None:intergenic
ACTAGCGTTATTTAATTCCG+CGG 0.642834 8.2:-82418193 None:intergenic
AGCTCAAGATTTCAAGACCG+CGG 0.654864 8.2:+82418176 MS.gene99166:CDS
ATTGTACACTGAGTAGACAA+TGG 0.673180 8.2:-82418505 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGAAAATATTGTAAACAA+AGG - chr8.2:82418013-82418032 None:intergenic 15.0%
!! GAAATGATCTTTGTTAAAAA+AGG + chr8.2:82418357-82418376 MS.gene99166:CDS 20.0%
!!! ATGAATATGGTTTGTTTATT+TGG - chr8.2:82418120-82418139 None:intergenic 20.0%
! AAACAAACCATATTCATCAA+TGG + chr8.2:82418123-82418142 MS.gene99166:CDS 25.0%
! AGAATCAATATCGATAATGA+TGG + chr8.2:82418300-82418319 MS.gene99166:CDS 25.0%
! TTCCATTACATTCTAAATCA+AGG + chr8.2:82418471-82418490 MS.gene99166:CDS 25.0%
!! AAAAAAGGTGTATTACTAGT+TGG + chr8.2:82418372-82418391 MS.gene99166:CDS 25.0%
!! AACCTTGATTTAGAATGTAA+TGG - chr8.2:82418476-82418495 None:intergenic 25.0%
!! ATGTAATGGAATAAACCTTT+AGG - chr8.2:82418462-82418481 None:intergenic 25.0%
!! TGTAATGGAATAAACCTTTA+GGG - chr8.2:82418461-82418480 None:intergenic 25.0%
!!! AATATGGTTTGTTTATTTGG+TGG - chr8.2:82418117-82418136 None:intergenic 25.0%
TTCCAAAATGTTCTCAAAGT+TGG + chr8.2:82418420-82418439 MS.gene99166:CDS 30.0%
! TGCTAATGAAACTTGGATTT+TGG - chr8.2:82418058-82418077 None:intergenic 30.0%
! TGTTTATTTGGTGGAATTGA+TGG - chr8.2:82418108-82418127 None:intergenic 30.0%
AAGATCATTTCAGTAGCTCT+TGG - chr8.2:82418348-82418367 None:intergenic 35.0%
AATGTTGTGTCAGAAGTTGT+AGG - chr8.2:82418561-82418580 None:intergenic 35.0%
ACTAGCGTTATTTAATTCCG+CGG - chr8.2:82418196-82418215 None:intergenic 35.0%
ATTGTACACTGAGTAGACAA+TGG - chr8.2:82418508-82418527 None:intergenic 35.0%
CACAATCTGCTAATGAAACT+TGG - chr8.2:82418065-82418084 None:intergenic 35.0%
GGAATAATAGATACTTGTCC+AGG - chr8.2:82418540-82418559 None:intergenic 35.0%
GTGTACAATAGTCAAAATCC+TGG + chr8.2:82418519-82418538 MS.gene99166:CDS 35.0%
TACAACTTCTGACACAACAT+TGG + chr8.2:82418560-82418579 MS.gene99166:CDS 35.0%
! ACATTAGCTGGATTTTTGCA+GGG - chr8.2:82418153-82418172 None:intergenic 35.0%
! GATGTTTGTGTTTTCCCTAA+AGG + chr8.2:82418444-82418463 MS.gene99166:CDS 35.0%
! TACATTAGCTGGATTTTTGC+AGG - chr8.2:82418154-82418173 None:intergenic 35.0%
!! CACCAACTTTGAGAACATTT+TGG - chr8.2:82418425-82418444 None:intergenic 35.0%
CAGTAGCTCTTGGATGATAA+TGG - chr8.2:82418338-82418357 None:intergenic 40.0%
CTTTCTGCTTCTCAAGTTCA+TGG + chr8.2:82418618-82418637 MS.gene99166:CDS 40.0%
GGGTAGACCATTGATGAATA+TGG - chr8.2:82418133-82418152 None:intergenic 40.0%
TCTTGAGCTGTTACATTAGC+TGG - chr8.2:82418165-82418184 None:intergenic 40.0%
!!! GCTAGTTCTACCGACATTTT+TGG + chr8.2:82418210-82418229 MS.gene99166:CDS 40.0%
AACACTCTTGGTCTCTCGAT+TGG + chr8.2:82418276-82418295 MS.gene99166:CDS 45.0%
AGCAGCAGTGACAAGTTTCA+CGG - chr8.2:82418241-82418260 None:intergenic 45.0%
AGCTCAAGATTTCAAGACCG+CGG + chr8.2:82418176-82418195 MS.gene99166:CDS 45.0%
TCACTGCTGCTGAATTTCCA+GGG + chr8.2:82418250-82418269 MS.gene99166:CDS 45.0%
CACGGATGCGCCAAAAATGT+CGG - chr8.2:82418223-82418242 None:intergenic 50.0%
GTCACTGCTGCTGAATTTCC+AGG + chr8.2:82418249-82418268 MS.gene99166:CDS 50.0%
TTTCCAGGGCTGAACACTCT+TGG + chr8.2:82418264-82418283 MS.gene99166:CDS 50.0%
!! AGACCAAGAGTGTTCAGCCC+TGG - chr8.2:82418270-82418289 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 82418003 82418665 82418003 ID=MS.gene99166
chr8.2 mRNA 82418003 82418665 82418003 ID=MS.gene99166.t1;Parent=MS.gene99166
chr8.2 exon 82418003 82418665 82418003 ID=MS.gene99166.t1.exon1;Parent=MS.gene99166.t1
chr8.2 CDS 82418003 82418665 82418003 ID=cds.MS.gene99166.t1;Parent=MS.gene99166.t1
Gene Sequence

>MS.gene99166

ATGAAATCCTTTGTTTACAATATTTTCTTTGTGTTCTTTTTTAATCTCTTAACCAAAATCCAAGTTTCATTAGCAGATTGTGACAACTTACAAGACACATGTCCATCAATTCCACCAAATAAACAAACCATATTCATCAATGGTCTACCCTGCAAAAATCCAGCTAATGTAACAGCTCAAGATTTCAAGACCGCGGAATTAAATAACGCTAGTTCTACCGACATTTTTGGCGCATCCGTGAAACTTGTCACTGCTGCTGAATTTCCAGGGCTGAACACTCTTGGTCTCTCGATTGGAAGAATCAATATCGATAATGATGGCTTAGTAAACTTCCATTATCATCCAAGAGCTACTGAAATGATCTTTGTTAAAAAAGGTGTATTACTAGTTGGATTTATTGATACACAAAATCAAGTTTTCCAAAATGTTCTCAAAGTTGGTGATGTTTGTGTTTTCCCTAAAGGTTTATTCCATTACATTCTAAATCAAGGTTTTGAAGAAGCCATTGTCTACTCAGTGTACAATAGTCAAAATCCTGGACAAGTATCTATTATTCCTACAACTTCTGACACAACATTGGAATCTGTAGAGAAGCTGAAAAAGAAAATTATCTCACTTTCTGCTTCTCAAGTTCATGGTGATATTATCATTTCAACCTCTTAG
Protein sequence

>MS.gene99166.t1

MKSFVYNIFFVFFFNLLTKIQVSLADCDNLQDTCPSIPPNKQTIFINGLPCKNPANVTAQDFKTAELNNASSTDIFGASVKLVTAAEFPGLNTLGLSIGRINIDNDGLVNFHYHPRATEMIFVKKGVLLVGFIDTQNQVFQNVLKVGDVCVFPKGLFHYILNQGFEEAIVYSVYNSQNPGQVSIIPTTSDTTLESVEKLKKKIISLSASQVHGDIIISTS