AlfalfaGEDB | MS.gene07591

M. sativa cultivar XinJiangDaYe / MS.gene07591

KEGG: -
GO: GO:0003674 GO:0005488 GO:0030145 GO:0043167 GO:0043169 GO:0045735 GO:0046872 GO:0046914
PFAM: PF00190.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07591.t1	XP_003590904.1	99.1	222	2	0	1	222	1	222	2.60E-122	448

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07591.t1	Q9FIC8	70.5	210	60	2	9	217	11	219	1.0e-79	297.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07591.t1	G7I5N4	99.1	222	2	0	1	222	1	222	1.9e-122	448.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049267	MS.gene07591	0.802435	5.62E-49	-1.69E-46
MS.gene049549	MS.gene07591	0.800596	1.34E-48	-1.69E-46
MS.gene049611	MS.gene07591	0.803578	3.26E-49	-1.69E-46
MS.gene050096	MS.gene07591	0.802494	5.46E-49	-1.69E-46
MS.gene05079	MS.gene07591	0.820641	6.16E-53	-1.69E-46
MS.gene050809	MS.gene07591	0.874834	4.95E-68	-1.69E-46
MS.gene050971	MS.gene07591	-0.811533	6.68E-51	-1.69E-46
MS.gene051632	MS.gene07591	0.805462	1.32E-49	-1.69E-46
MS.gene05240	MS.gene07591	0.811638	6.34E-51	-1.69E-46
MS.gene052702	MS.gene07591	0.81195	5.42E-51	-1.69E-46
MS.gene052805	MS.gene07591	0.824392	8.28E-54	-1.69E-46
MS.gene053420	MS.gene07591	0.805293	1.43E-49	-1.69E-46
MS.gene053449	MS.gene07591	-0.822253	2.62E-53	-1.69E-46
MS.gene053488	MS.gene07591	0.806655	7.40E-50	-1.69E-46
MS.gene053916	MS.gene07591	-0.825774	3.90E-54	-1.69E-46
MS.gene054181	MS.gene07591	0.825416	4.74E-54	-1.69E-46
MS.gene054290	MS.gene07591	0.86551	5.60E-65	-1.69E-46
MS.gene054742	MS.gene07591	0.800458	1.43E-48	-1.69E-46
MS.gene055959	MS.gene07591	0.85331	2.60E-61	-1.69E-46
MS.gene056511	MS.gene07591	-0.802209	6.25E-49	-1.69E-46
MS.gene056590	MS.gene07591	0.804991	1.66E-49	-1.69E-46
MS.gene056591	MS.gene07591	0.803652	3.15E-49	-1.69E-46
MS.gene056856	MS.gene07591	0.826111	3.24E-54	-1.69E-46
MS.gene057544	MS.gene07591	0.838273	3.17E-57	-1.69E-46
MS.gene057549	MS.gene07591	0.816441	5.52E-52	-1.69E-46
MS.gene057926	MS.gene07591	-0.805174	1.52E-49	-1.69E-46
MS.gene058067	MS.gene07591	0.850483	1.65E-60	-1.69E-46
MS.gene058068	MS.gene07591	0.818445	1.95E-52	-1.69E-46
MS.gene058093	MS.gene07591	0.851371	9.27E-61	-1.69E-46
MS.gene058099	MS.gene07591	0.818144	2.28E-52	-1.69E-46
MS.gene058179	MS.gene07591	0.820284	7.44E-53	-1.69E-46
MS.gene058269	MS.gene07591	0.803722	3.04E-49	-1.69E-46
MS.gene058840	MS.gene07591	0.823058	1.70E-53	-1.69E-46
MS.gene058928	MS.gene07591	-0.809344	1.98E-50	-1.69E-46
MS.gene059037	MS.gene07591	0.817111	3.91E-52	-1.69E-46
MS.gene059038	MS.gene07591	0.836457	9.25E-57	-1.69E-46
MS.gene059402	MS.gene07591	0.875904	2.13E-68	-1.69E-46
MS.gene059955	MS.gene07591	0.811821	5.78E-51	-1.69E-46
MS.gene060405	MS.gene07591	0.806105	9.67E-50	-1.69E-46
MS.gene060578	MS.gene07591	0.80043	1.45E-48	-1.69E-46
MS.gene060818	MS.gene07591	-0.816006	6.91E-52	-1.69E-46
MS.gene061041	MS.gene07591	-0.82214	2.78E-53	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene07591	MS.gene70061	PPI
MS.gene81739	MS.gene07591	PPI
MS.gene056870	MS.gene07591	PPI
MS.gene005041	MS.gene07591	PPI
MS.gene07591	MS.gene82857	PPI
MS.gene70061	MS.gene07591	PPI
MS.gene037516	MS.gene07591	PPI
MS.gene07591	MS.gene037518	PPI
MS.gene82857	MS.gene07591	PPI
MS.gene50790	MS.gene07591	PPI
MS.gene037519	MS.gene07591	PPI
MS.gene89773	MS.gene07591	PPI
MS.gene004411	MS.gene07591	PPI
MS.gene002728	MS.gene07591	PPI
MS.gene07591	MS.gene047464	PPI
MS.gene037517	MS.gene07591	PPI
MS.gene032596	MS.gene07591	PPI
MS.gene50789	MS.gene07591	PPI
MS.gene50788	MS.gene07591	PPI
MS.gene70064	MS.gene07591	PPI
MS.gene74887	MS.gene07591	PPI
MS.gene89772	MS.gene07591	PPI
MS.gene047464	MS.gene07591	PPI
MS.gene89774	MS.gene07591	PPI
MS.gene07591	MS.gene82855	PPI
MS.gene032598	MS.gene07591	PPI
MS.gene70063	MS.gene07591	PPI
MS.gene053033	MS.gene07591	PPI
MS.gene037520	MS.gene07591	PPI
MS.gene053027	MS.gene07591	PPI
MS.gene037851	MS.gene07591	PPI
MS.gene07591	MS.gene053031	PPI
MS.gene07591	MS.gene82859	PPI
MS.gene50787	MS.gene07591	PPI
MS.gene70062	MS.gene07591	PPI
MS.gene055423	MS.gene07591	PPI
MS.gene032599	MS.gene07591	PPI
MS.gene032601	MS.gene07591	PPI
MS.gene053029	MS.gene07591	PPI
MS.gene037518	MS.gene07591	PPI
MS.gene053030	MS.gene07591	PPI
MS.gene053026	MS.gene07591	PPI
MS.gene032597	MS.gene07591	PPI
MS.gene96244	MS.gene07591	PPI
MS.gene82859	MS.gene07591	PPI
MS.gene89775	MS.gene07591	PPI
MS.gene034877	MS.gene07591	PPI
MS.gene07591	MS.gene50789	PPI
MS.gene82858	MS.gene07591	PPI
MS.gene82855	MS.gene07591	PPI
MS.gene50786	MS.gene07591	PPI
MS.gene07591	MS.gene037520	PPI
MS.gene053031	MS.gene07591	PPI
MS.gene99166	MS.gene07591	PPI
MS.gene022634	MS.gene07591	PPI
MS.gene82860	MS.gene07591	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07591.t1	MTR_1g079490	99.099	222	2	0	1	222	1	222	9.11e-161	443
MS.gene07591.t1	MTR_4g017050	80.180	222	43	1	1	222	1	221	1.27e-129	364
MS.gene07591.t1	MTR_5g046410	80.180	222	43	1	1	222	1	221	1.99e-129	364
MS.gene07591.t1	MTR_5g046430	80.180	222	43	1	1	222	1	221	1.99e-129	364
MS.gene07591.t1	MTR_4g017030	80.180	222	43	1	1	222	1	221	1.99e-129	364
MS.gene07591.t1	MTR_4g017040	79.730	222	44	1	1	222	1	221	1.24e-128	362
MS.gene07591.t1	MTR_2g019250	78.829	222	46	1	1	222	1	221	2.48e-128	361
MS.gene07591.t1	MTR_6g005330	80.909	220	39	3	3	222	4	220	7.39e-125	352
MS.gene07591.t1	MTR_6g005310	82.407	216	35	3	6	221	7	219	1.61e-124	351
MS.gene07591.t1	MTR_6g005360	80.822	219	39	3	4	222	5	220	5.61e-124	350
MS.gene07591.t1	MTR_6g005340	81.567	217	37	3	6	222	7	220	9.82e-124	349
MS.gene07591.t1	MTR_6g005350	64.253	221	75	4	2	222	3	219	3.96e-92	269
MS.gene07591.t1	MTR_6g005380	63.942	208	73	2	2	209	3	208	8.08e-90	263
MS.gene07591.t1	MTR_1g023770	59.633	218	78	3	1	217	1	209	4.91e-84	248
MS.gene07591.t1	MTR_2g030855	64.500	200	64	4	19	217	19	212	1.71e-83	247
MS.gene07591.t1	MTR_2g031270	63.184	201	66	5	19	217	19	213	1.84e-80	239
MS.gene07591.t1	MTR_2g030895	65.896	173	56	3	47	217	7	178	1.60e-76	228
MS.gene07591.t1	MTR_2g030865	65.896	173	56	3	47	217	7	178	4.07e-75	225
MS.gene07591.t1	MTR_1g085500	46.948	213	107	3	5	216	6	213	1.43e-61	192
MS.gene07591.t1	MTR_2g031300	57.558	172	50	4	47	217	7	156	4.90e-58	181
MS.gene07591.t1	MTR_1g085140	47.500	200	99	3	19	217	21	215	1.64e-54	174
MS.gene07591.t1	MTR_7g111240	48.731	197	95	3	22	217	20	211	2.24e-53	171
MS.gene07591.t1	MTR_5g036570	47.980	198	96	4	22	217	27	219	3.83e-53	170
MS.gene07591.t1	MTR_7g111240	48.731	197	92	4	22	217	20	208	8.38e-51	164
MS.gene07591.t1	MTR_1g112200	44.279	201	97	6	19	217	20	207	5.71e-47	154
MS.gene07591.t1	MTR_1g060120	36.937	222	124	4	1	217	11	221	7.92e-42	142
MS.gene07591.t1	MTR_2g041550	41.905	210	106	5	6	214	9	203	1.14e-40	138
MS.gene07591.t1	MTR_8g020750	40.826	218	118	5	1	217	1	208	1.49e-39	135
MS.gene07591.t1	MTR_3g019710	38.389	211	122	3	7	217	3	205	7.61e-38	131
MS.gene07591.t1	MTR_2g041570	39.151	212	113	5	5	215	8	204	3.77e-37	129
MS.gene07591.t1	MTR_2g041580	39.336	211	112	5	5	214	8	203	4.93e-37	129
MS.gene07591.t1	MTR_8g020590	39.524	210	115	5	7	215	4	202	9.09e-37	128
MS.gene07591.t1	MTR_8g020630	38.144	194	114	2	7	200	3	190	1.05e-35	125
MS.gene07591.t1	MTR_8g007300	41.111	180	102	2	22	201	26	201	2.07e-35	125
MS.gene07591.t1	MTR_2g019780	36.697	218	127	5	1	217	1	208	7.69e-35	123
MS.gene07591.t1	MTR_8g020650	37.629	194	113	3	24	217	20	205	2.75e-34	122
MS.gene07591.t1	MTR_3g019680	38.660	194	111	3	24	217	19	204	3.77e-34	121
MS.gene07591.t1	MTR_3g019650	38.144	194	112	3	24	217	19	204	4.43e-34	121
MS.gene07591.t1	MTR_2g036120	38.235	204	117	3	7	207	3	200	5.51e-34	121
MS.gene07591.t1	MTR_2g045100	39.062	192	109	3	26	217	22	205	6.09e-33	118
MS.gene07591.t1	MTR_0284s0010	36.321	212	125	4	7	217	3	205	1.17e-32	117
MS.gene07591.t1	MTR_2g044040	35.545	211	128	3	7	217	3	205	1.76e-32	117
MS.gene07591.t1	MTR_4g010340	37.696	191	110	4	27	217	20	201	3.28e-32	116
MS.gene07591.t1	MTR_8g020670	35.122	205	121	4	13	217	13	205	1.05e-31	115
MS.gene07591.t1	MTR_5g031460	35.122	205	119	3	3	207	7	197	2.34e-31	114
MS.gene07591.t1	MTR_3g073170	39.062	192	103	5	28	217	27	206	6.42e-31	113
MS.gene07591.t1	MTR_8g020770	42.458	179	92	5	1	177	1	170	3.72e-30	111
MS.gene07591.t1	MTR_7g011340	37.500	192	112	3	26	217	22	205	4.42e-30	111
MS.gene07591.t1	MTR_8g020763	37.500	176	103	3	43	217	17	186	1.79e-29	108
MS.gene07591.t1	MTR_3g096330	35.859	198	119	2	4	201	559	748	4.11e-29	114
MS.gene07591.t1	MTR_8g020760	34.101	217	133	4	1	217	1	207	1.35e-28	107
MS.gene07591.t1	MTR_8g020610	34.742	213	126	6	6	217	3	203	3.06e-28	106
MS.gene07591.t1	MTR_2g086640	36.139	202	109	6	17	217	21	203	6.33e-28	105
MS.gene07591.t1	MTR_4g052780	40.278	144	80	3	75	217	67	205	9.78e-27	102
MS.gene07591.t1	MTR_2g086620	41.912	136	73	3	83	217	74	204	1.09e-26	102
MS.gene07591.t1	MTR_4g052770	40.278	144	80	3	75	217	67	205	2.06e-26	101
MS.gene07591.t1	MTR_2g086630	41.176	136	74	3	83	217	74	204	3.20e-26	100
MS.gene07591.t1	MTR_3g019810	32.178	202	123	5	7	207	109	297	2.97e-21	90.1
MS.gene07591.t1	MTR_4g027415	39.370	127	71	2	26	152	22	142	9.07e-19	79.7
MS.gene07591.t1	MTR_2g030915	47.368	76	25	1	94	169	1	61	1.43e-17	75.5
MS.gene07591.t1	MTR_2g072560	36.364	121	71	2	1	121	1	115	1.15e-14	68.9
MS.gene07591.t1	MTR_3g019610	40.952	105	60	1	7	111	3	105	1.20e-13	65.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07591.t1	AT3G05950	69.058	223	64	3	2	220	3	224	1.64e-101	293
MS.gene07591.t1	AT5G39130	70.476	210	60	2	9	217	11	219	2.25e-101	293
MS.gene07591.t1	AT5G39190	70.000	210	61	2	9	217	11	219	8.56e-101	291
MS.gene07591.t1	AT5G39160	70.000	210	61	2	9	217	11	219	9.25e-101	291
MS.gene07591.t1	AT5G39110	67.453	212	68	1	7	217	9	220	1.55e-98	286
MS.gene07591.t1	AT5G39150	66.667	213	70	1	6	217	7	219	3.29e-98	285
MS.gene07591.t1	AT5G39180	66.667	213	70	1	6	217	7	219	5.16e-98	285
MS.gene07591.t1	AT5G39120	68.966	203	62	1	16	217	17	219	2.03e-97	283
MS.gene07591.t1	AT5G39160	67.619	210	62	2	9	217	11	215	7.72e-96	279
MS.gene07591.t1	AT4G14630	64.186	215	76	1	4	217	8	222	3.18e-93	272
MS.gene07591.t1	AT3G04200	65.403	211	71	2	8	217	13	222	4.72e-89	262
MS.gene07591.t1	AT5G38940	67.005	197	63	2	22	217	26	221	8.08e-88	259
MS.gene07591.t1	AT5G38940	67.005	197	63	2	22	217	50	245	1.31e-87	259
MS.gene07591.t1	AT5G38930	67.839	199	62	2	20	217	24	221	1.74e-87	258
MS.gene07591.t1	AT5G38960	65.500	200	66	2	20	217	24	222	1.69e-86	255
MS.gene07591.t1	AT5G39190	62.201	209	57	1	9	217	11	197	4.74e-84	248
MS.gene07591.t1	AT5G39160	61.722	209	58	1	9	217	11	197	1.02e-83	247
MS.gene07591.t1	AT5G38910	60.465	215	83	2	4	217	6	219	6.82e-83	246
MS.gene07591.t1	AT3G04170	58.768	211	82	3	8	217	12	218	7.49e-81	241
MS.gene07591.t1	AT3G04190	60.294	204	79	2	15	217	19	221	5.83e-80	239
MS.gene07591.t1	AT3G04150	57.273	220	89	3	2	217	3	221	9.33e-80	238
MS.gene07591.t1	AT3G04150	54.741	232	89	3	2	217	3	234	5.28e-79	237
MS.gene07591.t1	AT3G04180	57.843	204	84	2	15	217	19	221	3.83e-76	229
MS.gene07591.t1	AT3G62020	49.074	216	102	5	4	217	6	215	1.73e-59	186
MS.gene07591.t1	AT5G38910	48.837	215	71	2	4	217	6	182	3.43e-59	184
MS.gene07591.t1	AT1G02335	46.009	213	109	3	6	217	8	215	3.56e-59	186
MS.gene07591.t1	AT1G09560	48.276	203	99	3	17	218	19	216	2.10e-58	184
MS.gene07591.t1	AT1G18970	48.500	200	95	4	19	217	26	218	1.16e-56	179
MS.gene07591.t1	AT1G18980	46.977	215	103	5	4	217	14	218	5.77e-56	177
MS.gene07591.t1	AT3G05930	44.444	216	106	4	4	217	12	215	1.18e-52	169
MS.gene07591.t1	AT3G62020	49.724	181	86	3	39	217	9	186	2.61e-51	165
MS.gene07591.t1	AT5G26700	46.114	193	95	4	26	217	25	209	2.65e-51	166
MS.gene07591.t1	AT1G10460	43.500	200	106	5	22	217	19	215	9.79e-50	162
MS.gene07591.t1	AT3G10080	41.176	204	108	4	19	217	23	219	1.64e-45	151
MS.gene07591.t1	AT3G10080	41.176	204	108	4	19	217	94	290	9.18e-45	151
MS.gene07591.t1	AT5G20630	42.718	206	104	6	5	209	8	200	3.49e-42	142
MS.gene07591.t1	AT1G74820	41.237	194	106	4	25	217	39	225	2.29e-39	135
MS.gene07591.t1	AT1G72610	44.318	176	92	3	26	200	19	189	1.91e-38	132
MS.gene07591.t1	AT5G61750	42.246	187	101	4	4	189	3	183	1.86e-37	130
MS.gene07591.t1	AT5G38950	61.972	71	27	0	146	216	2	72	2.29e-22	88.2
MS.gene07591.t1	AT5G39100	70.000	50	15	0	7	56	9	58	5.72e-18	75.1

Find 60 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTACTTCATCGTTGCCTTAT+TGG	0.169384	1.2:-61959693	MS.gene07591:CDS
GTGAGAACTTCCAAAGAAAT+TGG	0.253491	1.2:+61958865	None:intergenic
ATCTAAACCACCAATTTCTT+TGG	0.270284	1.2:-61958875	MS.gene07591:CDS
TACTGAGATTCTTGTAGTTT+TGG	0.280584	1.2:-61959103	MS.gene07591:CDS
CAAATATATACCACCAGTTT+TGG	0.298065	1.2:+61959598	None:intergenic
ACTATCTTCAAAAGCAATTC+TGG	0.307062	1.2:-61958810	MS.gene07591:CDS
CAACACACTTGGTATATCTT+TGG	0.313811	1.2:-61959181	MS.gene07591:CDS
TGAGGAGGATTTAGACCTTT+TGG	0.315163	1.2:+61959141	None:intergenic
GGGGAGGCTATTGCCATTGC+TGG	0.317939	1.2:-61958949	MS.gene07591:CDS
TTCCAATTGAACGTTGGTTA+TGG	0.322079	1.2:-61958970	MS.gene07591:CDS
TGGTAATCACTCCAGGGTTT+TGG	0.338241	1.2:+61958917	None:intergenic
GTGGCGGTTAATGAAATATT+AGG	0.343882	1.2:-61959207	MS.gene07591:CDS
ACCATAGCAAATGCTGTGTT+TGG	0.345841	1.2:-61958898	MS.gene07591:CDS
TCCAATTGAACGTTGGTTAT+GGG	0.359751	1.2:-61958969	MS.gene07591:CDS
TTTATCCACTTGAAAAGCTT+TGG	0.360291	1.2:+61958843	None:intergenic
GCGGATGATTTCTTCTTTCA+AGG	0.361596	1.2:-61959279	MS.gene07591:CDS
AGATGATGCTAAAGCCAATA+AGG	0.364373	1.2:+61959679	None:intergenic
TTGCAATGGGCTGGGATCAT+AGG	0.370729	1.2:+61959646	None:intergenic
GATTTCTTCTTTCAAGGATT+GGG	0.380699	1.2:-61959273	MS.gene07591:CDS
TGATTTCTTCTTTCAAGGAT+TGG	0.382095	1.2:-61959274	MS.gene07591:CDS
CCCCATAACCAACGTTCAAT+TGG	0.396722	1.2:+61958968	None:intergenic
ATCGCCTTTGTTCAATACTT+TGG	0.409827	1.2:+61959023	None:intergenic
TGGAAGTGAATGAGACCAAT+TGG	0.411740	1.2:+61958988	None:intergenic
TTCTTTCAAGGATTGGGACC+TGG	0.419003	1.2:-61959267	MS.gene07591:CDS
GATGTATTTGTGTTTCCAAT+TGG	0.426726	1.2:-61959003	MS.gene07591:CDS
ACAAAAGTCTTGCAATGGGC+TGG	0.433311	1.2:+61959637	None:intergenic
TAATTTCCAGTGTTCGTGAA+TGG	0.434819	1.2:-61959333	MS.gene07591:intron
TCTTTCAAGGATTGGGACCT+GGG	0.451301	1.2:-61959266	MS.gene07591:CDS
AATATTTCATTAACCGCCAC+TGG	0.460762	1.2:+61959210	None:intergenic
TTGGCTTTAGCATCATCTGT+TGG	0.476411	1.2:-61959674	MS.gene07591:CDS
GCAATCAATGATACCAAAAC+TGG	0.479248	1.2:-61959611	MS.gene07591:CDS
CAAAAGTCTTGCAATGGGCT+GGG	0.501275	1.2:+61959638	None:intergenic
ACGGTTGTTGTCTTGATTCG+AGG	0.503434	1.2:+61959053	None:intergenic
CCAATTGAACGTTGGTTATG+GGG	0.522282	1.2:-61958968	MS.gene07591:CDS
TCATCCGCATTTGCAAGCTT+AGG	0.527443	1.2:+61959294	None:intergenic
AGTGGATTTGAAGTATTCCC+AGG	0.530147	1.2:+61959249	None:intergenic
CAACACAAAAGTCTTGCAAT+GGG	0.530539	1.2:+61959633	None:intergenic
TTGGAAGGTACACTTTATGT+TGG	0.532815	1.2:-61959084	MS.gene07591:CDS
TTTGCTATGGTAATCACTCC+AGG	0.534925	1.2:+61958910	None:intergenic
AGAACTTCCAAAGAAATTGG+TGG	0.547357	1.2:+61958868	None:intergenic
GCAACACAAAAGTCTTGCAA+TGG	0.550725	1.2:+61959632	None:intergenic
TTGGCTACTAAGACCAGCAA+TGG	0.575368	1.2:+61958936	None:intergenic
AGATCCTAAGCTTGCAAATG+CGG	0.587730	1.2:-61959298	MS.gene07591:CDS
TCCAAACACAGCATTTGCTA+TGG	0.591300	1.2:+61958897	None:intergenic
TCAATACTTTGGTGAAGAGA+CGG	0.592996	1.2:+61959034	None:intergenic
ATTCACTTCCAATTGAACGT+TGG	0.603364	1.2:-61958976	MS.gene07591:CDS
ATATTAGGACTCAACACACT+TGG	0.605651	1.2:-61959192	MS.gene07591:CDS
TTGCTATGGTAATCACTCCA+GGG	0.606541	1.2:+61958911	None:intergenic
ATCAATGATACCAAAACTGG+TGG	0.616290	1.2:-61959608	MS.gene07591:intron
GTACCTCGAGGGTGAGTGTG+AGG	0.618466	1.2:+61959123	None:intergenic
ATTGAACGTTGGTTATGGGG+AGG	0.631460	1.2:-61958965	MS.gene07591:CDS
ATCAAAAGTGACTCCAGTGG+CGG	0.643268	1.2:-61959223	MS.gene07591:CDS
CCTCGAGGGTGAGTGTGAGG+AGG	0.646431	1.2:+61959126	None:intergenic
TTCACCAAAGTATTGAACAA+AGG	0.653228	1.2:-61959027	MS.gene07591:CDS
CAAGAATCTCAGTACCTCGA+GGG	0.659275	1.2:+61959112	None:intergenic
GGGAATACTTCAAATCCACT+CGG	0.669068	1.2:-61959246	MS.gene07591:CDS
GGTCTTAGTAGCCAAAACCC+TGG	0.671124	1.2:-61958928	MS.gene07591:CDS
CGGATCAAAAGTGACTCCAG+TGG	0.686899	1.2:-61959226	MS.gene07591:CDS
ACAAGAATCTCAGTACCTCG+AGG	0.695186	1.2:+61959111	None:intergenic
CCTCCTCACACTCACCCTCG+AGG	0.708362	1.2:-61959126	MS.gene07591:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTATTTATTTATTTTT+TGG	-	chr1.2:61959049-61959068	MS.gene07591:CDS	0.0%
!!!	AAATCAATTACCATAATTTT+AGG	+	chr1.2:61959032-61959051	None:intergenic	15.0%
!!!	ATTTTATACACCTAAAATTA+TGG	-	chr1.2:61959019-61959038	MS.gene07591:CDS	15.0%
!!	AAAAAACTATAATAACATGG+AGG	+	chr1.2:61958955-61958974	None:intergenic	20.0%
!!	CACAAAAAACTATAATAACA+TGG	+	chr1.2:61958958-61958977	None:intergenic	20.0%
!!	AATCAAGTTTTCTTGTGATT+TGG	-	chr1.2:61959138-61959157	MS.gene07591:CDS	25.0%
	ACTATCTTCAAAAGCAATTC+TGG	-	chr1.2:61959689-61959708	MS.gene07591:CDS	30.0%
	ATCTAAACCACCAATTTCTT+TGG	-	chr1.2:61959624-61959643	MS.gene07591:CDS	30.0%
	GATGTATTTGTGTTTCCAAT+TGG	-	chr1.2:61959496-61959515	MS.gene07591:intron	30.0%
	GATTTCTTCTTTCAAGGATT+GGG	-	chr1.2:61959226-61959245	MS.gene07591:CDS	30.0%
	TGATTTCTTCTTTCAAGGAT+TGG	-	chr1.2:61959225-61959244	MS.gene07591:CDS	30.0%
	TTTATCCACTTGAAAAGCTT+TGG	+	chr1.2:61959659-61959678	None:intergenic	30.0%
!	CAAATATATACCACCAGTTT+TGG	+	chr1.2:61958904-61958923	None:intergenic	30.0%
!	GATTGCATATCTAAAACATG+TGG	-	chr1.2:61958985-61959004	MS.gene07591:CDS	30.0%
!	TACTGAGATTCTTGTAGTTT+TGG	-	chr1.2:61959396-61959415	MS.gene07591:intron	30.0%
!!	TTCACCAAAGTATTGAACAA+AGG	-	chr1.2:61959472-61959491	MS.gene07591:intron	30.0%
	AATATTTCATTAACCGCCAC+TGG	+	chr1.2:61959292-61959311	None:intergenic	35.0%
	AGAACTTCCAAAGAAATTGG+TGG	+	chr1.2:61959634-61959653	None:intergenic	35.0%
	AGATGATGCTAAAGCCAATA+AGG	+	chr1.2:61958823-61958842	None:intergenic	35.0%
	ATATTAGGACTCAACACACT+TGG	-	chr1.2:61959307-61959326	MS.gene07591:CDS	35.0%
	ATCAATGATACCAAAACTGG+TGG	-	chr1.2:61958891-61958910	MS.gene07591:CDS	35.0%
	ATCGCCTTTGTTCAATACTT+TGG	+	chr1.2:61959479-61959498	None:intergenic	35.0%
	ATTCACTTCCAATTGAACGT+TGG	-	chr1.2:61959523-61959542	MS.gene07591:intron	35.0%
	CAACACAAAAGTCTTGCAAT+GGG	+	chr1.2:61958869-61958888	None:intergenic	35.0%
	CAACACACTTGGTATATCTT+TGG	-	chr1.2:61959318-61959337	MS.gene07591:CDS	35.0%
	GCAATCAATGATACCAAAAC+TGG	-	chr1.2:61958888-61958907	MS.gene07591:CDS	35.0%
	GTGAGAACTTCCAAAGAAAT+TGG	+	chr1.2:61959637-61959656	None:intergenic	35.0%
	GTGGCGGTTAATGAAATATT+AGG	-	chr1.2:61959292-61959311	MS.gene07591:CDS	35.0%
	TAATTTCCAGTGTTCGTGAA+TGG	-	chr1.2:61959166-61959185	MS.gene07591:CDS	35.0%
	TCAATACTTTGGTGAAGAGA+CGG	+	chr1.2:61959468-61959487	None:intergenic	35.0%
	TTACTTCATCGTTGCCTTAT+TGG	-	chr1.2:61958806-61958825	MS.gene07591:CDS	35.0%
!	AATTTTCCATTCACGAACAC+TGG	+	chr1.2:61959175-61959194	None:intergenic	35.0%
!	TCCAATTGAACGTTGGTTAT+GGG	-	chr1.2:61959530-61959549	MS.gene07591:intron	35.0%
!	TTCCAATTGAACGTTGGTTA+TGG	-	chr1.2:61959529-61959548	MS.gene07591:intron	35.0%
!	TTGGAAGGTACACTTTATGT+TGG	-	chr1.2:61959415-61959434	MS.gene07591:intron	35.0%
!!!	GAGATTCTTGTAGTTTTGGA+AGG	-	chr1.2:61959400-61959419	MS.gene07591:intron	35.0%
	ACCATAGCAAATGCTGTGTT+TGG	-	chr1.2:61959601-61959620	MS.gene07591:intron	40.0%
	AGATCCTAAGCTTGCAAATG+CGG	-	chr1.2:61959201-61959220	MS.gene07591:CDS	40.0%
	GCAACACAAAAGTCTTGCAA+TGG	+	chr1.2:61958870-61958889	None:intergenic	40.0%
	GCGGATGATTTCTTCTTTCA+AGG	-	chr1.2:61959220-61959239	MS.gene07591:CDS	40.0%
	GGGAATACTTCAAATCCACT+CGG	-	chr1.2:61959253-61959272	MS.gene07591:CDS	40.0%
	TGGAAGTGAATGAGACCAAT+TGG	+	chr1.2:61959514-61959533	None:intergenic	40.0%
	TTGCTATGGTAATCACTCCA+GGG	+	chr1.2:61959591-61959610	None:intergenic	40.0%
!	AGTGGATTTGAAGTATTCCC+AGG	+	chr1.2:61959253-61959272	None:intergenic	40.0%
!	CCAATTGAACGTTGGTTATG+GGG	-	chr1.2:61959531-61959550	MS.gene07591:intron	40.0%
!	TCCAAACACAGCATTTGCTA+TGG	+	chr1.2:61959605-61959624	None:intergenic	40.0%
!	TGAGGAGGATTTAGACCTTT+TGG	+	chr1.2:61959361-61959380	None:intergenic	40.0%
!	TTTGCTATGGTAATCACTCC+AGG	+	chr1.2:61959592-61959611	None:intergenic	40.0%
!!	TCTCACCAAAGCTTTTCAAG+TGG	-	chr1.2:61959651-61959670	MS.gene07591:CDS	40.0%
!!	TTGGCTTTAGCATCATCTGT+TGG	-	chr1.2:61958825-61958844	MS.gene07591:CDS	40.0%
	ACAAAAGTCTTGCAATGGGC+TGG	+	chr1.2:61958865-61958884	None:intergenic	45.0%
	ACAAGAATCTCAGTACCTCG+AGG	+	chr1.2:61959391-61959410	None:intergenic	45.0%
	ATCAAAAGTGACTCCAGTGG+CGG	-	chr1.2:61959276-61959295	MS.gene07591:CDS	45.0%
	CAAGAATCTCAGTACCTCGA+GGG	+	chr1.2:61959390-61959409	None:intergenic	45.0%
	CCCCATAACCAACGTTCAAT+TGG	+	chr1.2:61959534-61959553	None:intergenic	45.0%
	TCATCCGCATTTGCAAGCTT+AGG	+	chr1.2:61959208-61959227	None:intergenic	45.0%
	TCTTTCAAGGATTGGGACCT+GGG	-	chr1.2:61959233-61959252	MS.gene07591:CDS	45.0%
	TTCTTTCAAGGATTGGGACC+TGG	-	chr1.2:61959232-61959251	MS.gene07591:CDS	45.0%
	TTGGCTACTAAGACCAGCAA+TGG	+	chr1.2:61959566-61959585	None:intergenic	45.0%
!	ACGGTTGTTGTCTTGATTCG+AGG	+	chr1.2:61959449-61959468	None:intergenic	45.0%
!	ATTGAACGTTGGTTATGGGG+AGG	-	chr1.2:61959534-61959553	MS.gene07591:intron	45.0%
!	CAAAAGTCTTGCAATGGGCT+GGG	+	chr1.2:61958864-61958883	None:intergenic	45.0%
!	TGGTAATCACTCCAGGGTTT+TGG	+	chr1.2:61959585-61959604	None:intergenic	45.0%
!!	CTTTTGGTGCAAAATCGACG+CGG	+	chr1.2:61959345-61959364	None:intergenic	45.0%
!!	TTTTGGTGCAAAATCGACGC+GGG	+	chr1.2:61959344-61959363	None:intergenic	45.0%
	CGGATCAAAAGTGACTCCAG+TGG	-	chr1.2:61959273-61959292	MS.gene07591:CDS	50.0%
	GGTCTTAGTAGCCAAAACCC+TGG	-	chr1.2:61959571-61959590	MS.gene07591:intron	50.0%
!!	TTGCAATGGGCTGGGATCAT+AGG	+	chr1.2:61958856-61958875	None:intergenic	50.0%
!!!	CGCGTCGATTTTGCACCAAA+AGG	-	chr1.2:61959343-61959362	MS.gene07591:intron	50.0%
!!!	GGAGTCACTTTTGATCCGAG+TGG	+	chr1.2:61959271-61959290	None:intergenic	50.0%
	GGGGAGGCTATTGCCATTGC+TGG	-	chr1.2:61959550-61959569	MS.gene07591:intron	60.0%
	GTACCTCGAGGGTGAGTGTG+AGG	+	chr1.2:61959379-61959398	None:intergenic	60.0%
	CCTCCTCACACTCACCCTCG+AGG	-	chr1.2:61959373-61959392	MS.gene07591:intron	65.0%
	CCTCGAGGGTGAGTGTGAGG+AGG	+	chr1.2:61959376-61959395	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	61958795	61959726	61958795	ID=MS.gene07591
chr1.2	mRNA	61958795	61959726	61958795	ID=MS.gene07591.t1;Parent=MS.gene07591
chr1.2	exon	61959609	61959726	61959609	ID=MS.gene07591.t1.exon1;Parent=MS.gene07591.t1
chr1.2	CDS	61959609	61959726	61959609	ID=cds.MS.gene07591.t1;Parent=MS.gene07591.t1
chr1.2	exon	61958795	61959345	61958795	ID=MS.gene07591.t1.exon2;Parent=MS.gene07591.t1
chr1.2	CDS	61958795	61959345	61958795	ID=cds.MS.gene07591.t1;Parent=MS.gene07591.t1

Gene Sequence

>MS.gene07591

ATGGGAGTTTTTTACTTCATCGTTGCCTTATTGGCTTTAGCATCATCTGTTGGTTTTGCCTATGATCCCAGCCCATTGCAAGACTTTTGTGTTGCAATCAATGATACCAAAACTGGTGGTATATATTTGTTGTTCAATTGTTCAAAATAAATGAATACCTCCATGTTATTATAGTTTTTTGTGTATAAATGATTGCATATCTAAAACATGTGGTAAACTTGAGTATTTTATACACCTAAAATTATGGTAATTGATTTTTTATTTATTTATTTTTTGGTTAAATGATGATGAATTTTATGCGTCTATATTAACTAATTAGAAAGTGTCAAGAGTTTATTATATTAATCAAGTTTTCTTGTGATTTGGTTTTCTAATTTCCAGTGTTCGTGAATGGAAAATTTTGCAAAGATCCTAAGCTTGCAAATGCGGATGATTTCTTCTTTCAAGGATTGGGACCTGGGAATACTTCAAATCCACTCGGATCAAAAGTGACTCCAGTGGCGGTTAATGAAATATTAGGACTCAACACACTTGGTATATCTTTGGCCCGCGTCGATTTTGCACCAAAAGGTCTAAATCCTCCTCACACTCACCCTCGAGGTACTGAGATTCTTGTAGTTTTGGAAGGTACACTTTATGTTGGATTCGTTACCTCGAATCAAGACAACAACCGTCTCTTCACCAAAGTATTGAACAAAGGCGATGTATTTGTGTTTCCAATTGGTCTCATTCACTTCCAATTGAACGTTGGTTATGGGGAGGCTATTGCCATTGCTGGTCTTAGTAGCCAAAACCCTGGAGTGATTACCATAGCAAATGCTGTGTTTGGATCTAAACCACCAATTTCTTTGGAAGTTCTCACCAAAGCTTTTCAAGTGGATAAAAATGTAATCGACTATCTTCAAAAGCAATTCTGGTACAACAATAGTTAA

Protein sequence

>MS.gene07591.t1

MGVFYFIVALLALASSVGFAYDPSPLQDFCVAINDTKTGVFVNGKFCKDPKLANADDFFFQGLGPGNTSNPLGSKVTPVAVNEILGLNTLGISLARVDFAPKGLNPPHTHPRGTEILVVLEGTLYVGFVTSNQDNNRLFTKVLNKGDVFVFPIGLIHFQLNVGYGEAIAIAGLSSQNPGVITIANAVFGSKPPISLEVLTKAFQVDKNVIDYLQKQFWYNNS