Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89772.t1 | XP_003604729.1 | 98.6 | 221 | 3 | 0 | 1 | 221 | 1 | 221 | 2.10E-119 | 438.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89772.t1 | Q9SFF9 | 65.5 | 223 | 69 | 4 | 6 | 221 | 7 | 228 | 6.5e-74 | 278.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89772.t1 | G7JQ50 | 98.6 | 221 | 3 | 0 | 1 | 221 | 1 | 221 | 1.5e-119 | 438.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene89772 | 0.800025 | 1.75E-48 | -1.69E-46 |
| MS.gene049661 | MS.gene89772 | -0.809354 | 1.97E-50 | -1.69E-46 |
| MS.gene049662 | MS.gene89772 | -0.808472 | 3.05E-50 | -1.69E-46 |
| MS.gene050096 | MS.gene89772 | 0.808551 | 2.93E-50 | -1.69E-46 |
| MS.gene050464 | MS.gene89772 | -0.81051 | 1.11E-50 | -1.69E-46 |
| MS.gene050529 | MS.gene89772 | -0.80596 | 1.04E-49 | -1.69E-46 |
| MS.gene050971 | MS.gene89772 | -0.808987 | 2.36E-50 | -1.69E-46 |
| MS.gene051158 | MS.gene89772 | 0.819981 | 8.73E-53 | -1.69E-46 |
| MS.gene05240 | MS.gene89772 | 0.815028 | 1.14E-51 | -1.69E-46 |
| MS.gene05244 | MS.gene89772 | 0.801209 | 1.00E-48 | -1.69E-46 |
| MS.gene053031 | MS.gene89772 | 0.811662 | 6.26E-51 | -1.69E-46 |
| MS.gene053033 | MS.gene89772 | 0.81228 | 4.59E-51 | -1.69E-46 |
| MS.gene053406 | MS.gene89772 | 0.809585 | 1.76E-50 | -1.69E-46 |
| MS.gene054010 | MS.gene89772 | -0.801954 | 7.06E-49 | -1.69E-46 |
| MS.gene054181 | MS.gene89772 | 0.838826 | 2.28E-57 | -1.69E-46 |
| MS.gene054290 | MS.gene89772 | 0.836064 | 1.16E-56 | -1.69E-46 |
| MS.gene054742 | MS.gene89772 | 0.811038 | 8.55E-51 | -1.69E-46 |
| MS.gene055595 | MS.gene89772 | 0.801111 | 1.05E-48 | -1.69E-46 |
| MS.gene055597 | MS.gene89772 | 0.810599 | 1.06E-50 | -1.69E-46 |
| MS.gene055601 | MS.gene89772 | 0.854863 | 9.27E-62 | -1.69E-46 |
| MS.gene055959 | MS.gene89772 | 0.80143 | 9.04E-49 | -1.69E-46 |
| MS.gene056899 | MS.gene89772 | 0.848971 | 4.36E-60 | -1.69E-46 |
| MS.gene057544 | MS.gene89772 | 0.82598 | 3.49E-54 | -1.69E-46 |
| MS.gene058218 | MS.gene89772 | 0.858943 | 5.82E-63 | -1.69E-46 |
| MS.gene058928 | MS.gene89772 | -0.819341 | 1.22E-52 | -1.69E-46 |
| MS.gene059043 | MS.gene89772 | 0.821428 | 4.06E-53 | -1.69E-46 |
| MS.gene059294 | MS.gene89772 | -0.83893 | 2.15E-57 | -1.69E-46 |
| MS.gene059402 | MS.gene89772 | 0.867239 | 1.59E-65 | -1.69E-46 |
| MS.gene059955 | MS.gene89772 | 0.805438 | 1.33E-49 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89772.t1 | MTR_5g046410 | 98.643 | 221 | 3 | 0 | 1 | 221 | 1 | 221 | 1.80e-160 | 442 |
| MS.gene89772.t1 | MTR_5g046430 | 98.643 | 221 | 3 | 0 | 1 | 221 | 1 | 221 | 1.80e-160 | 442 |
| MS.gene89772.t1 | MTR_4g017030 | 98.643 | 221 | 3 | 0 | 1 | 221 | 1 | 221 | 1.80e-160 | 442 |
| MS.gene89772.t1 | MTR_4g017050 | 98.190 | 221 | 4 | 0 | 1 | 221 | 1 | 221 | 6.14e-160 | 441 |
| MS.gene89772.t1 | MTR_2g019250 | 96.380 | 221 | 8 | 0 | 1 | 221 | 1 | 221 | 3.12e-158 | 437 |
| MS.gene89772.t1 | MTR_4g017040 | 96.833 | 221 | 7 | 0 | 1 | 221 | 1 | 221 | 3.60e-158 | 436 |
| MS.gene89772.t1 | MTR_1g079490 | 81.081 | 222 | 41 | 1 | 1 | 221 | 1 | 222 | 2.45e-130 | 366 |
| MS.gene89772.t1 | MTR_6g005360 | 75.566 | 221 | 53 | 1 | 1 | 221 | 1 | 220 | 5.31e-119 | 337 |
| MS.gene89772.t1 | MTR_6g005340 | 77.477 | 222 | 47 | 3 | 1 | 221 | 1 | 220 | 6.05e-119 | 337 |
| MS.gene89772.t1 | MTR_6g005330 | 77.027 | 222 | 48 | 3 | 1 | 221 | 1 | 220 | 8.50e-119 | 337 |
| MS.gene89772.t1 | MTR_6g005310 | 77.376 | 221 | 47 | 3 | 1 | 220 | 1 | 219 | 2.18e-118 | 336 |
| MS.gene89772.t1 | MTR_6g005350 | 62.443 | 221 | 81 | 2 | 1 | 221 | 1 | 219 | 4.87e-91 | 266 |
| MS.gene89772.t1 | MTR_6g005380 | 61.058 | 208 | 81 | 0 | 1 | 208 | 1 | 208 | 3.10e-88 | 259 |
| MS.gene89772.t1 | MTR_1g023770 | 59.447 | 217 | 77 | 3 | 1 | 215 | 1 | 208 | 1.13e-81 | 243 |
| MS.gene89772.t1 | MTR_2g030855 | 60.500 | 200 | 69 | 4 | 19 | 215 | 19 | 211 | 7.31e-78 | 233 |
| MS.gene89772.t1 | MTR_2g031270 | 57.534 | 219 | 82 | 5 | 1 | 215 | 1 | 212 | 5.07e-74 | 223 |
| MS.gene89772.t1 | MTR_2g030895 | 64.162 | 173 | 56 | 4 | 47 | 215 | 7 | 177 | 1.59e-71 | 216 |
| MS.gene89772.t1 | MTR_2g030865 | 63.584 | 173 | 57 | 4 | 47 | 215 | 7 | 177 | 1.34e-69 | 211 |
| MS.gene89772.t1 | MTR_1g085500 | 46.047 | 215 | 109 | 3 | 3 | 215 | 4 | 213 | 2.12e-60 | 189 |
| MS.gene89772.t1 | MTR_1g085140 | 46.000 | 200 | 101 | 3 | 18 | 215 | 20 | 214 | 1.20e-53 | 171 |
| MS.gene89772.t1 | MTR_7g111240 | 47.449 | 196 | 96 | 3 | 22 | 215 | 20 | 210 | 4.29e-52 | 167 |
| MS.gene89772.t1 | MTR_2g031300 | 54.386 | 171 | 53 | 4 | 47 | 214 | 7 | 155 | 1.32e-51 | 164 |
| MS.gene89772.t1 | MTR_7g111240 | 47.449 | 196 | 93 | 3 | 22 | 215 | 20 | 207 | 7.75e-51 | 164 |
| MS.gene89772.t1 | MTR_5g036570 | 45.685 | 197 | 99 | 4 | 22 | 215 | 27 | 218 | 2.22e-49 | 160 |
| MS.gene89772.t1 | MTR_1g112200 | 41.346 | 208 | 90 | 7 | 19 | 215 | 20 | 206 | 2.10e-43 | 145 |
| MS.gene89772.t1 | MTR_2g041550 | 42.453 | 212 | 105 | 5 | 4 | 213 | 7 | 203 | 7.61e-43 | 144 |
| MS.gene89772.t1 | MTR_1g060120 | 36.652 | 221 | 128 | 5 | 2 | 215 | 5 | 220 | 3.17e-42 | 142 |
| MS.gene89772.t1 | MTR_2g041570 | 39.252 | 214 | 113 | 5 | 3 | 214 | 6 | 204 | 9.35e-40 | 135 |
| MS.gene89772.t1 | MTR_2g041580 | 39.437 | 213 | 112 | 5 | 3 | 213 | 6 | 203 | 1.04e-39 | 135 |
| MS.gene89772.t1 | MTR_8g007300 | 40.000 | 200 | 107 | 4 | 22 | 219 | 26 | 214 | 4.36e-36 | 127 |
| MS.gene89772.t1 | MTR_8g020590 | 38.889 | 216 | 116 | 6 | 1 | 214 | 1 | 202 | 6.36e-35 | 123 |
| MS.gene89772.t1 | MTR_8g020750 | 40.102 | 197 | 98 | 5 | 1 | 190 | 1 | 184 | 2.56e-34 | 122 |
| MS.gene89772.t1 | MTR_3g019710 | 39.378 | 193 | 107 | 5 | 7 | 197 | 3 | 187 | 9.99e-33 | 117 |
| MS.gene89772.t1 | MTR_8g020630 | 38.857 | 175 | 98 | 4 | 7 | 179 | 3 | 170 | 2.51e-32 | 117 |
| MS.gene89772.t1 | MTR_2g036120 | 36.967 | 211 | 117 | 6 | 1 | 206 | 1 | 200 | 1.62e-30 | 112 |
| MS.gene89772.t1 | MTR_8g020650 | 35.938 | 192 | 112 | 5 | 26 | 215 | 22 | 204 | 3.62e-30 | 111 |
| MS.gene89772.t1 | MTR_8g020770 | 40.782 | 179 | 94 | 4 | 1 | 176 | 1 | 170 | 3.94e-30 | 111 |
| MS.gene89772.t1 | MTR_8g020670 | 34.112 | 214 | 124 | 6 | 4 | 215 | 6 | 204 | 3.55e-29 | 108 |
| MS.gene89772.t1 | MTR_3g019650 | 39.655 | 174 | 95 | 5 | 26 | 197 | 21 | 186 | 5.96e-29 | 108 |
| MS.gene89772.t1 | MTR_3g019680 | 37.245 | 196 | 106 | 6 | 4 | 197 | 6 | 186 | 6.71e-29 | 107 |
| MS.gene89772.t1 | MTR_0284s0010 | 36.585 | 205 | 118 | 5 | 7 | 206 | 3 | 200 | 1.18e-28 | 107 |
| MS.gene89772.t1 | MTR_2g044040 | 36.416 | 173 | 101 | 4 | 7 | 177 | 3 | 168 | 5.48e-28 | 105 |
| MS.gene89772.t1 | MTR_2g086640 | 33.641 | 217 | 118 | 7 | 2 | 214 | 7 | 201 | 1.78e-27 | 104 |
| MS.gene89772.t1 | MTR_4g052780 | 35.784 | 204 | 105 | 6 | 16 | 214 | 21 | 203 | 1.89e-27 | 104 |
| MS.gene89772.t1 | MTR_2g045100 | 38.172 | 186 | 103 | 5 | 26 | 206 | 22 | 200 | 2.12e-27 | 103 |
| MS.gene89772.t1 | MTR_2g086620 | 41.985 | 131 | 70 | 3 | 85 | 214 | 77 | 202 | 2.57e-27 | 103 |
| MS.gene89772.t1 | MTR_3g096330 | 37.989 | 179 | 105 | 3 | 3 | 179 | 558 | 732 | 2.63e-27 | 109 |
| MS.gene89772.t1 | MTR_4g052770 | 35.294 | 204 | 106 | 6 | 16 | 214 | 21 | 203 | 5.20e-27 | 103 |
| MS.gene89772.t1 | MTR_2g086630 | 41.221 | 131 | 71 | 3 | 85 | 214 | 77 | 202 | 7.36e-27 | 102 |
| MS.gene89772.t1 | MTR_5g031460 | 34.286 | 210 | 124 | 5 | 1 | 206 | 1 | 200 | 2.92e-26 | 101 |
| MS.gene89772.t1 | MTR_8g020610 | 34.419 | 215 | 124 | 7 | 1 | 213 | 1 | 200 | 1.22e-25 | 99.4 |
| MS.gene89772.t1 | MTR_4g010340 | 34.703 | 219 | 119 | 7 | 1 | 215 | 2 | 200 | 7.48e-25 | 97.4 |
| MS.gene89772.t1 | MTR_8g020760 | 33.641 | 217 | 131 | 6 | 1 | 215 | 1 | 206 | 8.68e-25 | 97.4 |
| MS.gene89772.t1 | MTR_7g011340 | 37.097 | 186 | 105 | 5 | 26 | 206 | 22 | 200 | 1.41e-24 | 96.7 |
| MS.gene89772.t1 | MTR_8g020763 | 36.095 | 169 | 99 | 3 | 43 | 206 | 17 | 181 | 7.29e-24 | 94.4 |
| MS.gene89772.t1 | MTR_2g019780 | 32.420 | 219 | 132 | 7 | 1 | 215 | 1 | 207 | 2.20e-23 | 93.6 |
| MS.gene89772.t1 | MTR_3g073170 | 35.484 | 186 | 104 | 6 | 27 | 206 | 26 | 201 | 2.94e-23 | 93.2 |
| MS.gene89772.t1 | MTR_3g019810 | 33.659 | 205 | 114 | 9 | 1 | 202 | 107 | 292 | 2.27e-18 | 82.0 |
| MS.gene89772.t1 | MTR_2g030915 | 48.684 | 76 | 24 | 1 | 93 | 168 | 1 | 61 | 1.17e-17 | 75.5 |
| MS.gene89772.t1 | MTR_4g027415 | 37.500 | 128 | 71 | 4 | 26 | 151 | 22 | 142 | 1.29e-15 | 71.2 |
| MS.gene89772.t1 | MTR_2g072560 | 36.066 | 122 | 69 | 4 | 1 | 120 | 1 | 115 | 2.88e-12 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89772.t1 | AT3G05950 | 64.889 | 225 | 71 | 4 | 4 | 221 | 5 | 228 | 5.66e-96 | 280 |
| MS.gene89772.t1 | AT5G39150 | 64.929 | 211 | 71 | 2 | 7 | 215 | 9 | 218 | 2.53e-94 | 275 |
| MS.gene89772.t1 | AT5G39180 | 64.929 | 211 | 71 | 2 | 7 | 215 | 9 | 218 | 4.18e-94 | 275 |
| MS.gene89772.t1 | AT5G39120 | 64.929 | 211 | 71 | 2 | 7 | 215 | 9 | 218 | 4.32e-94 | 275 |
| MS.gene89772.t1 | AT5G39110 | 64.455 | 211 | 73 | 1 | 7 | 215 | 9 | 219 | 1.50e-92 | 271 |
| MS.gene89772.t1 | AT5G39160 | 66.029 | 209 | 68 | 2 | 9 | 215 | 11 | 218 | 2.27e-92 | 270 |
| MS.gene89772.t1 | AT5G39190 | 66.029 | 209 | 68 | 2 | 9 | 215 | 11 | 218 | 3.40e-92 | 270 |
| MS.gene89772.t1 | AT5G39130 | 65.072 | 209 | 70 | 2 | 9 | 215 | 11 | 218 | 7.38e-92 | 269 |
| MS.gene89772.t1 | AT4G14630 | 60.731 | 219 | 82 | 2 | 1 | 215 | 3 | 221 | 4.70e-88 | 259 |
| MS.gene89772.t1 | AT5G39160 | 63.636 | 209 | 69 | 2 | 9 | 215 | 11 | 214 | 1.25e-87 | 258 |
| MS.gene89772.t1 | AT3G04200 | 62.500 | 208 | 75 | 3 | 8 | 213 | 13 | 219 | 6.01e-84 | 249 |
| MS.gene89772.t1 | AT5G39190 | 59.903 | 207 | 62 | 1 | 9 | 215 | 11 | 196 | 4.12e-80 | 238 |
| MS.gene89772.t1 | AT5G39160 | 59.420 | 207 | 63 | 1 | 9 | 215 | 11 | 196 | 1.02e-79 | 237 |
| MS.gene89772.t1 | AT5G38910 | 59.545 | 220 | 82 | 4 | 1 | 215 | 1 | 218 | 3.37e-79 | 237 |
| MS.gene89772.t1 | AT5G38960 | 60.697 | 201 | 75 | 3 | 18 | 215 | 22 | 221 | 8.44e-78 | 233 |
| MS.gene89772.t1 | AT5G38940 | 60.714 | 196 | 74 | 2 | 22 | 215 | 26 | 220 | 1.51e-77 | 233 |
| MS.gene89772.t1 | AT5G38940 | 60.714 | 196 | 74 | 2 | 22 | 215 | 50 | 244 | 2.09e-77 | 233 |
| MS.gene89772.t1 | AT5G38930 | 61.616 | 198 | 73 | 2 | 20 | 215 | 24 | 220 | 2.28e-77 | 232 |
| MS.gene89772.t1 | AT3G04170 | 55.981 | 209 | 86 | 3 | 8 | 214 | 12 | 216 | 6.57e-75 | 226 |
| MS.gene89772.t1 | AT3G04190 | 56.219 | 201 | 85 | 2 | 15 | 213 | 19 | 218 | 6.88e-74 | 223 |
| MS.gene89772.t1 | AT3G04150 | 54.167 | 216 | 93 | 3 | 4 | 214 | 5 | 219 | 1.27e-72 | 220 |
| MS.gene89772.t1 | AT3G04150 | 51.316 | 228 | 94 | 3 | 4 | 214 | 5 | 232 | 8.66e-71 | 216 |
| MS.gene89772.t1 | AT3G04180 | 54.726 | 201 | 88 | 2 | 15 | 213 | 19 | 218 | 9.51e-71 | 215 |
| MS.gene89772.t1 | AT1G02335 | 43.458 | 214 | 114 | 3 | 4 | 215 | 6 | 214 | 6.16e-56 | 177 |
| MS.gene89772.t1 | AT5G38910 | 48.636 | 220 | 69 | 4 | 1 | 215 | 1 | 181 | 1.17e-55 | 176 |
| MS.gene89772.t1 | AT3G62020 | 46.009 | 213 | 106 | 5 | 6 | 215 | 8 | 214 | 5.95e-55 | 175 |
| MS.gene89772.t1 | AT1G09560 | 45.274 | 201 | 103 | 3 | 17 | 215 | 19 | 214 | 2.53e-53 | 171 |
| MS.gene89772.t1 | AT3G05930 | 47.525 | 202 | 97 | 4 | 4 | 202 | 6 | 201 | 1.50e-52 | 169 |
| MS.gene89772.t1 | AT1G18970 | 46.000 | 200 | 99 | 4 | 18 | 215 | 25 | 217 | 4.04e-51 | 165 |
| MS.gene89772.t1 | AT5G26700 | 46.875 | 192 | 88 | 5 | 26 | 213 | 25 | 206 | 1.21e-50 | 164 |
| MS.gene89772.t1 | AT1G18980 | 45.500 | 200 | 100 | 4 | 18 | 215 | 25 | 217 | 6.74e-49 | 159 |
| MS.gene89772.t1 | AT3G62020 | 46.667 | 180 | 90 | 3 | 39 | 215 | 9 | 185 | 2.31e-47 | 155 |
| MS.gene89772.t1 | AT3G10080 | 40.099 | 202 | 112 | 5 | 18 | 215 | 22 | 218 | 3.49e-44 | 147 |
| MS.gene89772.t1 | AT1G10460 | 41.414 | 198 | 108 | 5 | 22 | 214 | 19 | 213 | 3.88e-44 | 147 |
| MS.gene89772.t1 | AT3G10080 | 40.099 | 202 | 112 | 5 | 18 | 215 | 93 | 289 | 1.64e-43 | 148 |
| MS.gene89772.t1 | AT5G20630 | 42.381 | 210 | 109 | 6 | 1 | 208 | 1 | 200 | 4.01e-42 | 142 |
| MS.gene89772.t1 | AT5G61750 | 41.081 | 185 | 104 | 2 | 4 | 186 | 1 | 182 | 7.09e-40 | 136 |
| MS.gene89772.t1 | AT1G72610 | 41.053 | 190 | 103 | 6 | 26 | 213 | 19 | 201 | 3.91e-34 | 121 |
| MS.gene89772.t1 | AT1G74820 | 38.660 | 194 | 110 | 4 | 24 | 215 | 38 | 224 | 5.14e-33 | 119 |
| MS.gene89772.t1 | AT5G38950 | 54.167 | 72 | 33 | 0 | 145 | 216 | 2 | 73 | 1.32e-18 | 78.2 |
| MS.gene89772.t1 | AT5G39100 | 72.000 | 50 | 14 | 0 | 7 | 56 | 9 | 58 | 7.78e-18 | 74.7 |
Find 56 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATATACATACCACCATCTT+TGG | 0.255130 | 4.4:-83707827 | None:intergenic |
| TTTCAACTAAATGTTGGATA+TGG | 0.257371 | 4.4:+83708404 | MS.gene89772:CDS |
| CCTTGTTAGCATCTTGGCTT+TGG | 0.311083 | 4.4:+83707738 | MS.gene89772:CDS |
| CTTGTCAACTTGAAAAGCTT+TGG | 0.328967 | 4.4:-83708531 | None:intergenic |
| TTGAGATCCTAGTGCATTAG+AGG | 0.333822 | 4.4:-83708135 | None:intergenic |
| TAACACGCTTGGTATATCTT+TGG | 0.340502 | 4.4:+83708193 | MS.gene89772:CDS |
| TGAAAATGAATAAGACCAAT+TGG | 0.356438 | 4.4:-83708386 | None:intergenic |
| GAGTGCCTTCGAGTACTATA+AGG | 0.397511 | 4.4:-83708280 | None:intergenic |
| ATACTTCCTTGTTAGCATCT+TGG | 0.401215 | 4.4:+83707732 | MS.gene89772:CDS |
| TTCTTCAAACATGTTGAAGC+TGG | 0.402098 | 4.4:+83708107 | MS.gene89772:CDS |
| ATATACATACCACCATCTTT+GGG | 0.405165 | 4.4:-83707826 | None:intergenic |
| GGCAACGCTGTTGCTATTGC+TGG | 0.409649 | 4.4:+83708425 | MS.gene89772:CDS |
| CTCTAGGGTGGATGTGAGGT+GGG | 0.411548 | 4.4:-83708247 | None:intergenic |
| TTGGAGAGGGCTAGGGTCAT+AGG | 0.420416 | 4.4:-83707779 | None:intergenic |
| GATGTGTTTGTGTTCCCAAT+TGG | 0.424230 | 4.4:+83708371 | MS.gene89772:CDS |
| TAATTTGCAGTATTTGTCAA+TGG | 0.428669 | 4.4:+83708044 | MS.gene89772:intron |
| CAAAAGTCTTGGAGAGGGCT+AGG | 0.434671 | 4.4:-83707787 | None:intergenic |
| ATTATCTTCAAAAGCAGTCT+TGG | 0.442184 | 4.4:+83708564 | MS.gene89772:CDS |
| CTCGAAGGCACTCTTTATGT+TGG | 0.443268 | 4.4:+83708290 | MS.gene89772:CDS |
| TCTTCAGCTTTAACAAGTGC+GGG | 0.444792 | 4.4:-83708083 | None:intergenic |
| GTCACTGTAGACCAACTATT+CGG | 0.446134 | 4.4:+83708167 | MS.gene89772:CDS |
| AATTTGCAGTATTTGTCAAT+GGG | 0.453375 | 4.4:+83708045 | MS.gene89772:intron |
| ATAAGGATCTCTGTTCCTCT+AGG | 0.468428 | 4.4:-83708263 | None:intergenic |
| TTTGCAACGGTGATAACTCC+TGG | 0.470922 | 4.4:-83708464 | None:intergenic |
| TGATTGCAACACAAAAGTCT+TGG | 0.490396 | 4.4:-83707798 | None:intergenic |
| ATCACCCTTGTTGAGCACTT+TGG | 0.491510 | 4.4:-83708351 | None:intergenic |
| CCTCTAGGGTGGATGTGAGG+TGG | 0.496940 | 4.4:-83708248 | None:intergenic |
| TCTTCAAACATGTTGAAGCT+GGG | 0.501315 | 4.4:+83708108 | MS.gene89772:CDS |
| CTATTCGGACTTAACACGCT+TGG | 0.510323 | 4.4:+83708182 | MS.gene89772:CDS |
| GAAAATGAATAAGACCAATT+GGG | 0.510497 | 4.4:-83708385 | None:intergenic |
| AGAACTTCGTCGGAAATAAG+AGG | 0.511108 | 4.4:-83708506 | None:intergenic |
| AATAGTTGGTCTACAGTGAC+TGG | 0.527501 | 4.4:-83708164 | None:intergenic |
| GCGTGTTAAGTCCGAATAGT+TGG | 0.534502 | 4.4:-83708178 | None:intergenic |
| TGAGGTGGGTTTAAACCCTT+AGG | 0.537084 | 4.4:-83708233 | None:intergenic |
| ATCTTCAGCTTTAACAAGTG+CGG | 0.543612 | 4.4:-83708084 | None:intergenic |
| GCAATCAAAGACCCCAAAGA+TGG | 0.554146 | 4.4:+83707814 | MS.gene89772:CDS |
| AGCTTTGGTAAGAACTTCGT+CGG | 0.555884 | 4.4:-83708516 | None:intergenic |
| CCAAAGCCAAGATGCTAACA+AGG | 0.556137 | 4.4:-83707738 | None:intergenic |
| AAAAGTCTTGGAGAGGGCTA+GGG | 0.564154 | 4.4:-83707786 | None:intergenic |
| TAAGGATCTCTGTTCCTCTA+GGG | 0.571738 | 4.4:-83708262 | None:intergenic |
| GGACTTAGCAGTCAAAATCC+AGG | 0.578792 | 4.4:+83708446 | MS.gene89772:CDS |
| GGGAATGCCTCTAATGCACT+AGG | 0.589101 | 4.4:+83708128 | MS.gene89772:CDS |
| GGATCTCTGTTCCTCTAGGG+TGG | 0.590427 | 4.4:-83708259 | None:intergenic |
| TTTAAACAAAGCATTTGCAA+CGG | 0.590900 | 4.4:-83708477 | None:intergenic |
| CTTCACCAAAGTGCTCAACA+AGG | 0.595909 | 4.4:+83708346 | MS.gene89772:CDS |
| AGGGCTAGGGTCATAGGCAA+AGG | 0.609226 | 4.4:-83707773 | None:intergenic |
| ATCAAAGACCCCAAAGATGG+TGG | 0.628410 | 4.4:+83707817 | MS.gene89772:CDS |
| GTCATAGGCAAAGGCAACAG+AGG | 0.637895 | 4.4:-83707764 | None:intergenic |
| TATACATACCACCATCTTTG+GGG | 0.639620 | 4.4:-83707825 | None:intergenic |
| CAACACAAAAGTCTTGGAGA+GGG | 0.641422 | 4.4:-83707792 | None:intergenic |
| TTCACCAAAGTGCTCAACAA+GGG | 0.645837 | 4.4:+83708347 | MS.gene89772:CDS |
| GTTCCTCTAGGGTGGATGTG+AGG | 0.650118 | 4.4:-83708251 | None:intergenic |
| GCAACACAAAAGTCTTGGAG+AGG | 0.651085 | 4.4:-83707793 | None:intergenic |
| CCACCTCACATCCACCCTAG+AGG | 0.660499 | 4.4:+83708248 | MS.gene89772:CDS |
| CTTCAGCTTTAACAAGTGCG+GGG | 0.662073 | 4.4:-83708082 | None:intergenic |
| GAGATCCTTATAGTACTCGA+AGG | 0.666965 | 4.4:+83708275 | MS.gene89772:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTAATTACAACTAAATTTTT+AGG | + | chr4.4:83707867-83707886 | MS.gene89772:intron | 10.0% |
| !! | GTAATTAAGACTTAAAGTTA+AGG | - | chr4.4:83707855-83707874 | None:intergenic | 20.0% |
| !! | TTTAAATATGCAAATATTCG+AGG | + | chr4.4:83707890-83707909 | MS.gene89772:intron | 20.0% |
| !!! | ATTCATTTTCAACTAAATGT+TGG | + | chr4.4:83708398-83708417 | MS.gene89772:CDS | 20.0% |
| !!! | CAATATAGAATCTTTTGTTT+TGG | + | chr4.4:83707932-83707951 | MS.gene89772:intron | 20.0% |
| ! | AATTTGCAGTATTTGTCAAT+GGG | + | chr4.4:83708045-83708064 | MS.gene89772:intron | 25.0% |
| ! | CAAAAGATTCTATATTGACA+AGG | - | chr4.4:83707929-83707948 | None:intergenic | 25.0% |
| ! | GAAAATGAATAAGACCAATT+GGG | - | chr4.4:83708388-83708407 | None:intergenic | 25.0% |
| ! | TGAAAATGAATAAGACCAAT+TGG | - | chr4.4:83708389-83708408 | None:intergenic | 25.0% |
| ! | TTTCAACTAAATGTTGGATA+TGG | + | chr4.4:83708404-83708423 | MS.gene89772:CDS | 25.0% |
| !! | TAATTTGCAGTATTTGTCAA+TGG | + | chr4.4:83708044-83708063 | MS.gene89772:intron | 25.0% |
| !! | TTTAAACAAAGCATTTGCAA+CGG | - | chr4.4:83708480-83708499 | None:intergenic | 25.0% |
| ATATACATACCACCATCTTT+GGG | - | chr4.4:83707829-83707848 | None:intergenic | 30.0% | |
| ATTATCTTCAAAAGCAGTCT+TGG | + | chr4.4:83708564-83708583 | MS.gene89772:CDS | 30.0% | |
| TATATACATACCACCATCTT+TGG | - | chr4.4:83707830-83707849 | None:intergenic | 30.0% | |
| ! | TATGCAAATATTCGAGGTTT+TGG | + | chr4.4:83707896-83707915 | MS.gene89772:intron | 30.0% |
| !!! | GTTTTTTTGTTTAGCTAGCT+AGG | + | chr4.4:83707963-83707982 | MS.gene89772:intron | 30.0% |
| ATACTTCCTTGTTAGCATCT+TGG | + | chr4.4:83707732-83707751 | MS.gene89772:CDS | 35.0% | |
| CTTGTCAACTTGAAAAGCTT+TGG | - | chr4.4:83708534-83708553 | None:intergenic | 35.0% | |
| TAACACGCTTGGTATATCTT+TGG | + | chr4.4:83708193-83708212 | MS.gene89772:CDS | 35.0% | |
| TATACATACCACCATCTTTG+GGG | - | chr4.4:83707828-83707847 | None:intergenic | 35.0% | |
| TCTTCAAACATGTTGAAGCT+GGG | + | chr4.4:83708108-83708127 | MS.gene89772:CDS | 35.0% | |
| TGATTGCAACACAAAAGTCT+TGG | - | chr4.4:83707801-83707820 | None:intergenic | 35.0% | |
| TTCTTCAAACATGTTGAAGC+TGG | + | chr4.4:83708107-83708126 | MS.gene89772:CDS | 35.0% | |
| ! | ATCTTCAGCTTTAACAAGTG+CGG | - | chr4.4:83708087-83708106 | None:intergenic | 35.0% |
| AGAACTTCGTCGGAAATAAG+AGG | - | chr4.4:83708509-83708528 | None:intergenic | 40.0% | |
| ATAAGGATCTCTGTTCCTCT+AGG | - | chr4.4:83708266-83708285 | None:intergenic | 40.0% | |
| CAACACAAAAGTCTTGGAGA+GGG | - | chr4.4:83707795-83707814 | None:intergenic | 40.0% | |
| GAGATCCTTATAGTACTCGA+AGG | + | chr4.4:83708275-83708294 | MS.gene89772:CDS | 40.0% | |
| GATGTGTTTGTGTTCCCAAT+TGG | + | chr4.4:83708371-83708390 | MS.gene89772:CDS | 40.0% | |
| GTCACTGTAGACCAACTATT+CGG | + | chr4.4:83708167-83708186 | MS.gene89772:CDS | 40.0% | |
| TAAGGATCTCTGTTCCTCTA+GGG | - | chr4.4:83708265-83708284 | None:intergenic | 40.0% | |
| TTGAGATCCTAGTGCATTAG+AGG | - | chr4.4:83708138-83708157 | None:intergenic | 40.0% | |
| ! | AATAGTTGGTCTACAGTGAC+TGG | - | chr4.4:83708167-83708186 | None:intergenic | 40.0% |
| ! | AGCTTTGGTAAGAACTTCGT+CGG | - | chr4.4:83708519-83708538 | None:intergenic | 40.0% |
| ! | TCTTCAGCTTTAACAAGTGC+GGG | - | chr4.4:83708086-83708105 | None:intergenic | 40.0% |
| ! | TTCACCAAAGTGCTCAACAA+GGG | + | chr4.4:83708347-83708366 | MS.gene89772:CDS | 40.0% |
| AAAAGTCTTGGAGAGGGCTA+GGG | - | chr4.4:83707789-83707808 | None:intergenic | 45.0% | |
| ATCAAAGACCCCAAAGATGG+TGG | + | chr4.4:83707817-83707836 | MS.gene89772:CDS | 45.0% | |
| ATCACCCTTGTTGAGCACTT+TGG | - | chr4.4:83708354-83708373 | None:intergenic | 45.0% | |
| CCAAAGCCAAGATGCTAACA+AGG | - | chr4.4:83707741-83707760 | None:intergenic | 45.0% | |
| CCTTGTTAGCATCTTGGCTT+TGG | + | chr4.4:83707738-83707757 | MS.gene89772:CDS | 45.0% | |
| CTATTCGGACTTAACACGCT+TGG | + | chr4.4:83708182-83708201 | MS.gene89772:CDS | 45.0% | |
| GAGTGCCTTCGAGTACTATA+AGG | - | chr4.4:83708283-83708302 | None:intergenic | 45.0% | |
| GCAACACAAAAGTCTTGGAG+AGG | - | chr4.4:83707796-83707815 | None:intergenic | 45.0% | |
| GCAATCAAAGACCCCAAAGA+TGG | + | chr4.4:83707814-83707833 | MS.gene89772:CDS | 45.0% | |
| GCGTGTTAAGTCCGAATAGT+TGG | - | chr4.4:83708181-83708200 | None:intergenic | 45.0% | |
| GGACTTAGCAGTCAAAATCC+AGG | + | chr4.4:83708446-83708465 | MS.gene89772:CDS | 45.0% | |
| TTTGCAACGGTGATAACTCC+TGG | - | chr4.4:83708467-83708486 | None:intergenic | 45.0% | |
| ! | CTTCACCAAAGTGCTCAACA+AGG | + | chr4.4:83708346-83708365 | MS.gene89772:CDS | 45.0% |
| ! | CTTCAGCTTTAACAAGTGCG+GGG | - | chr4.4:83708085-83708104 | None:intergenic | 45.0% |
| ! | TGAGGTGGGTTTAAACCCTT+AGG | - | chr4.4:83708236-83708255 | None:intergenic | 45.0% |
| !! | CTCGAAGGCACTCTTTATGT+TGG | + | chr4.4:83708290-83708309 | MS.gene89772:CDS | 45.0% |
| CAAAAGTCTTGGAGAGGGCT+AGG | - | chr4.4:83707790-83707809 | None:intergenic | 50.0% | |
| GGGAATGCCTCTAATGCACT+AGG | + | chr4.4:83708128-83708147 | MS.gene89772:CDS | 50.0% | |
| GTCATAGGCAAAGGCAACAG+AGG | - | chr4.4:83707767-83707786 | None:intergenic | 50.0% | |
| !!! | CGCGTTGATTTTGCGCCTAA+GGG | + | chr4.4:83708218-83708237 | MS.gene89772:CDS | 50.0% |
| !!! | TCGCGTTGATTTTGCGCCTA+AGG | + | chr4.4:83708217-83708236 | MS.gene89772:CDS | 50.0% |
| AGGGCTAGGGTCATAGGCAA+AGG | - | chr4.4:83707776-83707795 | None:intergenic | 55.0% | |
| TTGGAGAGGGCTAGGGTCAT+AGG | - | chr4.4:83707782-83707801 | None:intergenic | 55.0% | |
| ! | GGATCTCTGTTCCTCTAGGG+TGG | - | chr4.4:83708262-83708281 | None:intergenic | 55.0% |
| ! | GGCAACGCTGTTGCTATTGC+TGG | + | chr4.4:83708425-83708444 | MS.gene89772:CDS | 55.0% |
| !! | CTCTAGGGTGGATGTGAGGT+GGG | - | chr4.4:83708250-83708269 | None:intergenic | 55.0% |
| !! | GTTCCTCTAGGGTGGATGTG+AGG | - | chr4.4:83708254-83708273 | None:intergenic | 55.0% |
| CCACCTCACATCCACCCTAG+AGG | + | chr4.4:83708248-83708267 | MS.gene89772:CDS | 60.0% | |
| !! | CCTCTAGGGTGGATGTGAGG+TGG | - | chr4.4:83708251-83708270 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 83707721 | 83708601 | 83707721 | ID=MS.gene89772 |
| chr4.4 | mRNA | 83707721 | 83708601 | 83707721 | ID=MS.gene89772.t1;Parent=MS.gene89772 |
| chr4.4 | exon | 83707721 | 83707838 | 83707721 | ID=MS.gene89772.t1.exon1;Parent=MS.gene89772.t1 |
| chr4.4 | CDS | 83707721 | 83707838 | 83707721 | ID=cds.MS.gene89772.t1;Parent=MS.gene89772.t1 |
| chr4.4 | exon | 83708054 | 83708601 | 83708054 | ID=MS.gene89772.t1.exon2;Parent=MS.gene89772.t1 |
| chr4.4 | CDS | 83708054 | 83708601 | 83708054 | ID=cds.MS.gene89772.t1;Parent=MS.gene89772.t1 |
| Gene Sequence |
| Protein sequence |
