AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene005041

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005041.t1 MTR_1g079490 99.099 222 2 0 1 222 1 222 9.11e-161 443
MS.gene005041.t1 MTR_4g017050 80.180 222 43 1 1 222 1 221 1.27e-129 364
MS.gene005041.t1 MTR_5g046410 80.180 222 43 1 1 222 1 221 1.99e-129 364
MS.gene005041.t1 MTR_5g046430 80.180 222 43 1 1 222 1 221 1.99e-129 364
MS.gene005041.t1 MTR_4g017030 80.180 222 43 1 1 222 1 221 1.99e-129 364
MS.gene005041.t1 MTR_4g017040 79.730 222 44 1 1 222 1 221 1.24e-128 362
MS.gene005041.t1 MTR_2g019250 78.829 222 46 1 1 222 1 221 2.48e-128 361
MS.gene005041.t1 MTR_6g005330 80.909 220 39 3 3 222 4 220 7.39e-125 352
MS.gene005041.t1 MTR_6g005310 82.407 216 35 3 6 221 7 219 1.61e-124 351
MS.gene005041.t1 MTR_6g005360 80.822 219 39 3 4 222 5 220 5.61e-124 350
MS.gene005041.t1 MTR_6g005340 81.567 217 37 3 6 222 7 220 9.82e-124 349
MS.gene005041.t1 MTR_6g005350 64.253 221 75 4 2 222 3 219 3.96e-92 269
MS.gene005041.t1 MTR_6g005380 63.942 208 73 2 2 209 3 208 8.08e-90 263
MS.gene005041.t1 MTR_1g023770 59.633 218 78 3 1 217 1 209 4.91e-84 248
MS.gene005041.t1 MTR_2g030855 64.500 200 64 4 19 217 19 212 1.71e-83 247
MS.gene005041.t1 MTR_2g031270 63.184 201 66 5 19 217 19 213 1.84e-80 239
MS.gene005041.t1 MTR_2g030895 65.896 173 56 3 47 217 7 178 1.60e-76 228
MS.gene005041.t1 MTR_2g030865 65.896 173 56 3 47 217 7 178 4.07e-75 225
MS.gene005041.t1 MTR_1g085500 46.948 213 107 3 5 216 6 213 1.43e-61 192
MS.gene005041.t1 MTR_2g031300 57.558 172 50 4 47 217 7 156 4.90e-58 181
MS.gene005041.t1 MTR_1g085140 47.500 200 99 3 19 217 21 215 1.64e-54 174
MS.gene005041.t1 MTR_7g111240 48.731 197 95 3 22 217 20 211 2.24e-53 171
MS.gene005041.t1 MTR_5g036570 47.980 198 96 4 22 217 27 219 3.83e-53 170
MS.gene005041.t1 MTR_7g111240 48.731 197 92 4 22 217 20 208 8.38e-51 164
MS.gene005041.t1 MTR_1g112200 44.279 201 97 6 19 217 20 207 5.71e-47 154
MS.gene005041.t1 MTR_1g060120 36.937 222 124 4 1 217 11 221 7.92e-42 142
MS.gene005041.t1 MTR_2g041550 41.905 210 106 5 6 214 9 203 1.14e-40 138
MS.gene005041.t1 MTR_8g020750 40.826 218 118 5 1 217 1 208 1.49e-39 135
MS.gene005041.t1 MTR_3g019710 38.389 211 122 3 7 217 3 205 7.61e-38 131
MS.gene005041.t1 MTR_2g041570 39.151 212 113 5 5 215 8 204 3.77e-37 129
MS.gene005041.t1 MTR_2g041580 39.336 211 112 5 5 214 8 203 4.93e-37 129
MS.gene005041.t1 MTR_8g020590 39.524 210 115 5 7 215 4 202 9.09e-37 128
MS.gene005041.t1 MTR_8g020630 38.144 194 114 2 7 200 3 190 1.05e-35 125
MS.gene005041.t1 MTR_8g007300 41.111 180 102 2 22 201 26 201 2.07e-35 125
MS.gene005041.t1 MTR_2g019780 36.697 218 127 5 1 217 1 208 7.69e-35 123
MS.gene005041.t1 MTR_8g020650 37.629 194 113 3 24 217 20 205 2.75e-34 122
MS.gene005041.t1 MTR_3g019680 38.660 194 111 3 24 217 19 204 3.77e-34 121
MS.gene005041.t1 MTR_3g019650 38.144 194 112 3 24 217 19 204 4.43e-34 121
MS.gene005041.t1 MTR_2g036120 38.235 204 117 3 7 207 3 200 5.51e-34 121
MS.gene005041.t1 MTR_2g045100 39.062 192 109 3 26 217 22 205 6.09e-33 118
MS.gene005041.t1 MTR_0284s0010 36.321 212 125 4 7 217 3 205 1.17e-32 117
MS.gene005041.t1 MTR_2g044040 35.545 211 128 3 7 217 3 205 1.76e-32 117
MS.gene005041.t1 MTR_4g010340 37.696 191 110 4 27 217 20 201 3.28e-32 116
MS.gene005041.t1 MTR_8g020670 35.122 205 121 4 13 217 13 205 1.05e-31 115
MS.gene005041.t1 MTR_5g031460 35.122 205 119 3 3 207 7 197 2.34e-31 114
MS.gene005041.t1 MTR_3g073170 39.062 192 103 5 28 217 27 206 6.42e-31 113
MS.gene005041.t1 MTR_8g020770 42.458 179 92 5 1 177 1 170 3.72e-30 111
MS.gene005041.t1 MTR_7g011340 37.500 192 112 3 26 217 22 205 4.42e-30 111
MS.gene005041.t1 MTR_8g020763 37.500 176 103 3 43 217 17 186 1.79e-29 108
MS.gene005041.t1 MTR_3g096330 35.859 198 119 2 4 201 559 748 4.11e-29 114
MS.gene005041.t1 MTR_8g020760 34.101 217 133 4 1 217 1 207 1.35e-28 107
MS.gene005041.t1 MTR_8g020610 34.742 213 126 6 6 217 3 203 3.06e-28 106
MS.gene005041.t1 MTR_2g086640 36.139 202 109 6 17 217 21 203 6.33e-28 105
MS.gene005041.t1 MTR_4g052780 40.278 144 80 3 75 217 67 205 9.78e-27 102
MS.gene005041.t1 MTR_2g086620 41.912 136 73 3 83 217 74 204 1.09e-26 102
MS.gene005041.t1 MTR_4g052770 40.278 144 80 3 75 217 67 205 2.06e-26 101
MS.gene005041.t1 MTR_2g086630 41.176 136 74 3 83 217 74 204 3.20e-26 100
MS.gene005041.t1 MTR_3g019810 32.178 202 123 5 7 207 109 297 2.97e-21 90.1
MS.gene005041.t1 MTR_4g027415 39.370 127 71 2 26 152 22 142 9.07e-19 79.7
MS.gene005041.t1 MTR_2g030915 47.368 76 25 1 94 169 1 61 1.43e-17 75.5
MS.gene005041.t1 MTR_2g072560 36.364 121 71 2 1 121 1 115 1.15e-14 68.9
MS.gene005041.t1 MTR_3g019610 40.952 105 60 1 7 111 3 105 1.20e-13 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005041.t1 AT3G05950 69.058 223 64 3 2 220 3 224 1.64e-101 293
MS.gene005041.t1 AT5G39130 70.476 210 60 2 9 217 11 219 2.25e-101 293
MS.gene005041.t1 AT5G39190 70.000 210 61 2 9 217 11 219 8.56e-101 291
MS.gene005041.t1 AT5G39160 70.000 210 61 2 9 217 11 219 9.25e-101 291
MS.gene005041.t1 AT5G39110 67.453 212 68 1 7 217 9 220 1.55e-98 286
MS.gene005041.t1 AT5G39150 66.667 213 70 1 6 217 7 219 3.29e-98 285
MS.gene005041.t1 AT5G39180 66.667 213 70 1 6 217 7 219 5.16e-98 285
MS.gene005041.t1 AT5G39120 68.966 203 62 1 16 217 17 219 2.03e-97 283
MS.gene005041.t1 AT5G39160 67.619 210 62 2 9 217 11 215 7.72e-96 279
MS.gene005041.t1 AT4G14630 64.186 215 76 1 4 217 8 222 3.18e-93 272
MS.gene005041.t1 AT3G04200 65.403 211 71 2 8 217 13 222 4.72e-89 262
MS.gene005041.t1 AT5G38940 67.005 197 63 2 22 217 26 221 8.08e-88 259
MS.gene005041.t1 AT5G38940 67.005 197 63 2 22 217 50 245 1.31e-87 259
MS.gene005041.t1 AT5G38930 67.839 199 62 2 20 217 24 221 1.74e-87 258
MS.gene005041.t1 AT5G38960 65.500 200 66 2 20 217 24 222 1.69e-86 255
MS.gene005041.t1 AT5G39190 62.201 209 57 1 9 217 11 197 4.74e-84 248
MS.gene005041.t1 AT5G39160 61.722 209 58 1 9 217 11 197 1.02e-83 247
MS.gene005041.t1 AT5G38910 60.465 215 83 2 4 217 6 219 6.82e-83 246
MS.gene005041.t1 AT3G04170 58.768 211 82 3 8 217 12 218 7.49e-81 241
MS.gene005041.t1 AT3G04190 60.294 204 79 2 15 217 19 221 5.83e-80 239
MS.gene005041.t1 AT3G04150 57.273 220 89 3 2 217 3 221 9.33e-80 238
MS.gene005041.t1 AT3G04150 54.741 232 89 3 2 217 3 234 5.28e-79 237
MS.gene005041.t1 AT3G04180 57.843 204 84 2 15 217 19 221 3.83e-76 229
MS.gene005041.t1 AT3G62020 49.074 216 102 5 4 217 6 215 1.73e-59 186
MS.gene005041.t1 AT5G38910 48.837 215 71 2 4 217 6 182 3.43e-59 184
MS.gene005041.t1 AT1G02335 46.009 213 109 3 6 217 8 215 3.56e-59 186
MS.gene005041.t1 AT1G09560 48.276 203 99 3 17 218 19 216 2.10e-58 184
MS.gene005041.t1 AT1G18970 48.500 200 95 4 19 217 26 218 1.16e-56 179
MS.gene005041.t1 AT1G18980 46.977 215 103 5 4 217 14 218 5.77e-56 177
MS.gene005041.t1 AT3G05930 44.444 216 106 4 4 217 12 215 1.18e-52 169
MS.gene005041.t1 AT3G62020 49.724 181 86 3 39 217 9 186 2.61e-51 165
MS.gene005041.t1 AT5G26700 46.114 193 95 4 26 217 25 209 2.65e-51 166
MS.gene005041.t1 AT1G10460 43.500 200 106 5 22 217 19 215 9.79e-50 162
MS.gene005041.t1 AT3G10080 41.176 204 108 4 19 217 23 219 1.64e-45 151
MS.gene005041.t1 AT3G10080 41.176 204 108 4 19 217 94 290 9.18e-45 151
MS.gene005041.t1 AT5G20630 42.718 206 104 6 5 209 8 200 3.49e-42 142
MS.gene005041.t1 AT1G74820 41.237 194 106 4 25 217 39 225 2.29e-39 135
MS.gene005041.t1 AT1G72610 44.318 176 92 3 26 200 19 189 1.91e-38 132
MS.gene005041.t1 AT5G61750 42.246 187 101 4 4 189 3 183 1.86e-37 130
MS.gene005041.t1 AT5G38950 61.972 71 27 0 146 216 2 72 2.29e-22 88.2
MS.gene005041.t1 AT5G39100 70.000 50 15 0 7 56 9 58 5.72e-18 75.1

Find 60 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACTTCATCGTTGCCTTAT+TGG 0.169384 1.4:+64313216 MS.gene005041:CDS
GTGAGAACTTCCAAAGAAAT+TGG 0.253491 1.4:-64314044 None:intergenic
ATCTAAACCACCAATTTCTT+TGG 0.270284 1.4:+64314034 MS.gene005041:CDS
TACTGAGATTCTTGTAGTTT+TGG 0.280584 1.4:+64313806 MS.gene005041:CDS
CAAATATATACCACCAGTTT+TGG 0.298065 1.4:-64313311 None:intergenic
ACTATCTTCAAAAGCAATTC+TGG 0.307062 1.4:+64314099 MS.gene005041:CDS
CAACACACTTGGTATATCTT+TGG 0.313811 1.4:+64313728 MS.gene005041:CDS
TGAGGAGGATTTAGACCTTT+TGG 0.315163 1.4:-64313768 None:intergenic
GGGGAGGCTATTGCCATTGC+TGG 0.317939 1.4:+64313960 MS.gene005041:CDS
TTCCAATTGAACGTTGGTTA+TGG 0.322079 1.4:+64313939 MS.gene005041:CDS
TGGTAATCACTCCAGGGTTT+TGG 0.338241 1.4:-64313992 None:intergenic
GTGGCGGTTAATGAAATATT+AGG 0.343882 1.4:+64313702 MS.gene005041:CDS
ACCATAGCAAATGCTGTGTT+TGG 0.345841 1.4:+64314011 MS.gene005041:CDS
TCCAATTGAACGTTGGTTAT+GGG 0.359751 1.4:+64313940 MS.gene005041:CDS
TTTATCCACTTGAAAAGCTT+TGG 0.360291 1.4:-64314066 None:intergenic
GCGGATGATTTCTTCTTTCA+AGG 0.361596 1.4:+64313630 MS.gene005041:CDS
AGATGATGCTAAAGCCAATA+AGG 0.364373 1.4:-64313230 None:intergenic
TTGCAATGGGCTGGGATCAT+AGG 0.370729 1.4:-64313263 None:intergenic
GATTTCTTCTTTCAAGGATT+GGG 0.380699 1.4:+64313636 MS.gene005041:CDS
TGATTTCTTCTTTCAAGGAT+TGG 0.382095 1.4:+64313635 MS.gene005041:CDS
CCCCATAACCAACGTTCAAT+TGG 0.396722 1.4:-64313941 None:intergenic
ATCGCCTTTGTTCAATACTT+TGG 0.409827 1.4:-64313886 None:intergenic
TGGAAGTGAATGAGACCAAT+TGG 0.411740 1.4:-64313921 None:intergenic
TTCTTTCAAGGATTGGGACC+TGG 0.419003 1.4:+64313642 MS.gene005041:CDS
GATGTATTTGTGTTTCCAAT+TGG 0.426726 1.4:+64313906 MS.gene005041:CDS
ACAAAAGTCTTGCAATGGGC+TGG 0.433311 1.4:-64313272 None:intergenic
TAATTTCCAGTGTTCGTGAA+TGG 0.434819 1.4:+64313576 MS.gene005041:intron
TCTTTCAAGGATTGGGACCT+GGG 0.451301 1.4:+64313643 MS.gene005041:CDS
AATATTTCATTAACCGCCAC+TGG 0.460762 1.4:-64313699 None:intergenic
TTGGCTTTAGCATCATCTGT+TGG 0.476411 1.4:+64313235 MS.gene005041:CDS
GCAATCAATGATACCAAAAC+TGG 0.479248 1.4:+64313298 MS.gene005041:CDS
CAAAAGTCTTGCAATGGGCT+GGG 0.501275 1.4:-64313271 None:intergenic
ACGGTTGTTGTCTTGATTCG+AGG 0.503434 1.4:-64313856 None:intergenic
CCAATTGAACGTTGGTTATG+GGG 0.522282 1.4:+64313941 MS.gene005041:CDS
TCATCCGCATTTGCAAGCTT+AGG 0.527443 1.4:-64313615 None:intergenic
AGTGGATTTGAAGTATTCCC+AGG 0.530147 1.4:-64313660 None:intergenic
CAACACAAAAGTCTTGCAAT+GGG 0.530539 1.4:-64313276 None:intergenic
TTGGAAGGTACACTTTATGT+TGG 0.532815 1.4:+64313825 MS.gene005041:CDS
TTTGCTATGGTAATCACTCC+AGG 0.534925 1.4:-64313999 None:intergenic
AGAACTTCCAAAGAAATTGG+TGG 0.547357 1.4:-64314041 None:intergenic
GCAACACAAAAGTCTTGCAA+TGG 0.550725 1.4:-64313277 None:intergenic
TTGGCTACTAAGACCAGCAA+TGG 0.575368 1.4:-64313973 None:intergenic
AGATCCTAAGCTTGCAAATG+CGG 0.587730 1.4:+64313611 MS.gene005041:CDS
TCCAAACACAGCATTTGCTA+TGG 0.591300 1.4:-64314012 None:intergenic
TCAATACTTTGGTGAAGAGA+CGG 0.592996 1.4:-64313875 None:intergenic
ATTCACTTCCAATTGAACGT+TGG 0.603364 1.4:+64313933 MS.gene005041:CDS
ATATTAGGACTCAACACACT+TGG 0.605651 1.4:+64313717 MS.gene005041:CDS
TTGCTATGGTAATCACTCCA+GGG 0.606541 1.4:-64313998 None:intergenic
ATCAATGATACCAAAACTGG+TGG 0.616290 1.4:+64313301 MS.gene005041:CDS
GTACCTCGAGGGTGAGTGTG+AGG 0.618466 1.4:-64313786 None:intergenic
ATTGAACGTTGGTTATGGGG+AGG 0.631460 1.4:+64313944 MS.gene005041:CDS
ATCAAAAGTGACTCCAGTGG+CGG 0.643268 1.4:+64313686 MS.gene005041:CDS
CCTCGAGGGTGAGTGTGAGG+AGG 0.646431 1.4:-64313783 None:intergenic
TTCACCAAAGTATTGAACAA+AGG 0.653228 1.4:+64313882 MS.gene005041:CDS
CAAGAATCTCAGTACCTCGA+GGG 0.659275 1.4:-64313797 None:intergenic
GGGAATACTTCAAATCCACT+CGG 0.669068 1.4:+64313663 MS.gene005041:CDS
GGTCTTAGTAGCCAAAACCC+TGG 0.671124 1.4:+64313981 MS.gene005041:CDS
CGGATCAAAAGTGACTCCAG+TGG 0.686899 1.4:+64313683 MS.gene005041:CDS
ACAAGAATCTCAGTACCTCG+AGG 0.695186 1.4:-64313798 None:intergenic
CCTCCTCACACTCACCCTCG+AGG 0.708362 1.4:+64313783 MS.gene005041:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTATTTATTTATTTTT+TGG + chr1.4:64313459-64313478 MS.gene005041:intron 0.0%
!!! AAATCAATTACCATAATTTT+AGG - chr1.4:64313442-64313461 None:intergenic 15.0%
!!! ATTTTATACACCTAAAATTA+TGG + chr1.4:64313429-64313448 MS.gene005041:intron 15.0%
!! AAAAAACTATAATAACATGG+AGG - chr1.4:64313365-64313384 None:intergenic 20.0%
!! CACAAAAAACTATAATAACA+TGG - chr1.4:64313368-64313387 None:intergenic 20.0%
!! AATCAAGTTTTCTTGTGATT+TGG + chr1.4:64313548-64313567 MS.gene005041:intron 25.0%
ACTATCTTCAAAAGCAATTC+TGG + chr1.4:64314099-64314118 MS.gene005041:CDS 30.0%
ATCTAAACCACCAATTTCTT+TGG + chr1.4:64314034-64314053 MS.gene005041:CDS 30.0%
GATGTATTTGTGTTTCCAAT+TGG + chr1.4:64313906-64313925 MS.gene005041:CDS 30.0%
GATTTCTTCTTTCAAGGATT+GGG + chr1.4:64313636-64313655 MS.gene005041:CDS 30.0%
TGATTTCTTCTTTCAAGGAT+TGG + chr1.4:64313635-64313654 MS.gene005041:CDS 30.0%
TTTATCCACTTGAAAAGCTT+TGG - chr1.4:64314069-64314088 None:intergenic 30.0%
! CAAATATATACCACCAGTTT+TGG - chr1.4:64313314-64313333 None:intergenic 30.0%
! GATTGCATATCTAAAACATG+TGG + chr1.4:64313395-64313414 MS.gene005041:intron 30.0%
! TACTGAGATTCTTGTAGTTT+TGG + chr1.4:64313806-64313825 MS.gene005041:CDS 30.0%
!! TTCACCAAAGTATTGAACAA+AGG + chr1.4:64313882-64313901 MS.gene005041:CDS 30.0%
AATATTTCATTAACCGCCAC+TGG - chr1.4:64313702-64313721 None:intergenic 35.0%
AGAACTTCCAAAGAAATTGG+TGG - chr1.4:64314044-64314063 None:intergenic 35.0%
AGATGATGCTAAAGCCAATA+AGG - chr1.4:64313233-64313252 None:intergenic 35.0%
ATATTAGGACTCAACACACT+TGG + chr1.4:64313717-64313736 MS.gene005041:CDS 35.0%
ATCAATGATACCAAAACTGG+TGG + chr1.4:64313301-64313320 MS.gene005041:CDS 35.0%
ATCGCCTTTGTTCAATACTT+TGG - chr1.4:64313889-64313908 None:intergenic 35.0%
ATTCACTTCCAATTGAACGT+TGG + chr1.4:64313933-64313952 MS.gene005041:CDS 35.0%
CAACACAAAAGTCTTGCAAT+GGG - chr1.4:64313279-64313298 None:intergenic 35.0%
CAACACACTTGGTATATCTT+TGG + chr1.4:64313728-64313747 MS.gene005041:CDS 35.0%
GCAATCAATGATACCAAAAC+TGG + chr1.4:64313298-64313317 MS.gene005041:CDS 35.0%
GTGAGAACTTCCAAAGAAAT+TGG - chr1.4:64314047-64314066 None:intergenic 35.0%
GTGGCGGTTAATGAAATATT+AGG + chr1.4:64313702-64313721 MS.gene005041:CDS 35.0%
TAATTTCCAGTGTTCGTGAA+TGG + chr1.4:64313576-64313595 MS.gene005041:intron 35.0%
TCAATACTTTGGTGAAGAGA+CGG - chr1.4:64313878-64313897 None:intergenic 35.0%
TTACTTCATCGTTGCCTTAT+TGG + chr1.4:64313216-64313235 MS.gene005041:CDS 35.0%
! AATTTTCCATTCACGAACAC+TGG - chr1.4:64313585-64313604 None:intergenic 35.0%
! TCCAATTGAACGTTGGTTAT+GGG + chr1.4:64313940-64313959 MS.gene005041:CDS 35.0%
! TTCCAATTGAACGTTGGTTA+TGG + chr1.4:64313939-64313958 MS.gene005041:CDS 35.0%
! TTGGAAGGTACACTTTATGT+TGG + chr1.4:64313825-64313844 MS.gene005041:CDS 35.0%
!!! GAGATTCTTGTAGTTTTGGA+AGG + chr1.4:64313810-64313829 MS.gene005041:CDS 35.0%
ACCATAGCAAATGCTGTGTT+TGG + chr1.4:64314011-64314030 MS.gene005041:CDS 40.0%
AGATCCTAAGCTTGCAAATG+CGG + chr1.4:64313611-64313630 MS.gene005041:CDS 40.0%
GCAACACAAAAGTCTTGCAA+TGG - chr1.4:64313280-64313299 None:intergenic 40.0%
GCGGATGATTTCTTCTTTCA+AGG + chr1.4:64313630-64313649 MS.gene005041:CDS 40.0%
GGGAATACTTCAAATCCACT+CGG + chr1.4:64313663-64313682 MS.gene005041:CDS 40.0%
TGGAAGTGAATGAGACCAAT+TGG - chr1.4:64313924-64313943 None:intergenic 40.0%
TTGCTATGGTAATCACTCCA+GGG - chr1.4:64314001-64314020 None:intergenic 40.0%
! AGTGGATTTGAAGTATTCCC+AGG - chr1.4:64313663-64313682 None:intergenic 40.0%
! CCAATTGAACGTTGGTTATG+GGG + chr1.4:64313941-64313960 MS.gene005041:CDS 40.0%
! TCCAAACACAGCATTTGCTA+TGG - chr1.4:64314015-64314034 None:intergenic 40.0%
! TGAGGAGGATTTAGACCTTT+TGG - chr1.4:64313771-64313790 None:intergenic 40.0%
! TTTGCTATGGTAATCACTCC+AGG - chr1.4:64314002-64314021 None:intergenic 40.0%
!! TCTCACCAAAGCTTTTCAAG+TGG + chr1.4:64314061-64314080 MS.gene005041:CDS 40.0%
!! TTGGCTTTAGCATCATCTGT+TGG + chr1.4:64313235-64313254 MS.gene005041:CDS 40.0%
ACAAAAGTCTTGCAATGGGC+TGG - chr1.4:64313275-64313294 None:intergenic 45.0%
ACAAGAATCTCAGTACCTCG+AGG - chr1.4:64313801-64313820 None:intergenic 45.0%
ATCAAAAGTGACTCCAGTGG+CGG + chr1.4:64313686-64313705 MS.gene005041:CDS 45.0%
CAAGAATCTCAGTACCTCGA+GGG - chr1.4:64313800-64313819 None:intergenic 45.0%
CCCCATAACCAACGTTCAAT+TGG - chr1.4:64313944-64313963 None:intergenic 45.0%
TCATCCGCATTTGCAAGCTT+AGG - chr1.4:64313618-64313637 None:intergenic 45.0%
TCTTTCAAGGATTGGGACCT+GGG + chr1.4:64313643-64313662 MS.gene005041:CDS 45.0%
TTCTTTCAAGGATTGGGACC+TGG + chr1.4:64313642-64313661 MS.gene005041:CDS 45.0%
TTGGCTACTAAGACCAGCAA+TGG - chr1.4:64313976-64313995 None:intergenic 45.0%
! ACGGTTGTTGTCTTGATTCG+AGG - chr1.4:64313859-64313878 None:intergenic 45.0%
! ATTGAACGTTGGTTATGGGG+AGG + chr1.4:64313944-64313963 MS.gene005041:CDS 45.0%
! CAAAAGTCTTGCAATGGGCT+GGG - chr1.4:64313274-64313293 None:intergenic 45.0%
! TGGTAATCACTCCAGGGTTT+TGG - chr1.4:64313995-64314014 None:intergenic 45.0%
!! CTTTTGGTGCAAAATCGACG+CGG - chr1.4:64313755-64313774 None:intergenic 45.0%
!! TTTTGGTGCAAAATCGACGC+GGG - chr1.4:64313754-64313773 None:intergenic 45.0%
CGGATCAAAAGTGACTCCAG+TGG + chr1.4:64313683-64313702 MS.gene005041:CDS 50.0%
GGTCTTAGTAGCCAAAACCC+TGG + chr1.4:64313981-64314000 MS.gene005041:CDS 50.0%
!! TTGCAATGGGCTGGGATCAT+AGG - chr1.4:64313266-64313285 None:intergenic 50.0%
!!! CGCGTCGATTTTGCACCAAA+AGG + chr1.4:64313753-64313772 MS.gene005041:CDS 50.0%
!!! GGAGTCACTTTTGATCCGAG+TGG - chr1.4:64313681-64313700 None:intergenic 50.0%
GGGGAGGCTATTGCCATTGC+TGG + chr1.4:64313960-64313979 MS.gene005041:CDS 60.0%
GTACCTCGAGGGTGAGTGTG+AGG - chr1.4:64313789-64313808 None:intergenic 60.0%
CCTCCTCACACTCACCCTCG+AGG + chr1.4:64313783-64313802 MS.gene005041:CDS 65.0%
CCTCGAGGGTGAGTGTGAGG+AGG - chr1.4:64313786-64313805 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 64313205 64314136 64313205 ID=MS.gene005041
chr1.4 mRNA 64313205 64314136 64313205 ID=MS.gene005041.t1;Parent=MS.gene005041
chr1.4 exon 64313205 64313322 64313205 ID=MS.gene005041.t1.exon1;Parent=MS.gene005041.t1
chr1.4 CDS 64313205 64313322 64313205 ID=cds.MS.gene005041.t1;Parent=MS.gene005041.t1
chr1.4 exon 64313586 64314136 64313586 ID=MS.gene005041.t1.exon2;Parent=MS.gene005041.t1
chr1.4 CDS 64313586 64314136 64313586 ID=cds.MS.gene005041.t1;Parent=MS.gene005041.t1
Gene Sequence

>MS.gene005041

ATGGGAGTTTTTTACTTCATCGTTGCCTTATTGGCTTTAGCATCATCTGTTGGTTTTGCCTATGATCCCAGCCCATTGCAAGACTTTTGTGTTGCAATCAATGATACCAAAACTGGTGGTATATATTTGTTGTTCAATTGTTCAAAATAAATGAATACCTCCATGTTATTATAGTTTTTTGTGTATAAATGATTGCATATCTAAAACATGTGGTAAACTTGAGTATTTTATACACCTAAAATTATGGTAATTGATTTTTTATTTATTTATTTTTTGGTTAAATGATGATGAATTTTATGCGTCTATATTAACTAATTAGAAAGTGTCAAGAGTTTATTATATTAATCAAGTTTTCTTGTGATTTGGTTTTCTAATTTCCAGTGTTCGTGAATGGAAAATTTTGCAAAGATCCTAAGCTTGCAAATGCGGATGATTTCTTCTTTCAAGGATTGGGACCTGGGAATACTTCAAATCCACTCGGATCAAAAGTGACTCCAGTGGCGGTTAATGAAATATTAGGACTCAACACACTTGGTATATCTTTGGCCCGCGTCGATTTTGCACCAAAAGGTCTAAATCCTCCTCACACTCACCCTCGAGGTACTGAGATTCTTGTAGTTTTGGAAGGTACACTTTATGTTGGATTCGTTACCTCGAATCAAGACAACAACCGTCTCTTCACCAAAGTATTGAACAAAGGCGATGTATTTGTGTTTCCAATTGGTCTCATTCACTTCCAATTGAACGTTGGTTATGGGGAGGCTATTGCCATTGCTGGTCTTAGTAGCCAAAACCCTGGAGTGATTACCATAGCAAATGCTGTGTTTGGATCTAAACCACCAATTTCTTTGGAAGTTCTCACCAAAGCTTTTCAAGTGGATAAAAATGTAATCGACTATCTTCAAAAGCAATTCTGGTACAACAATAGTTAA
Protein sequence

>MS.gene005041.t1

MGVFYFIVALLALASSVGFAYDPSPLQDFCVAINDTKTGVFVNGKFCKDPKLANADDFFFQGLGPGNTSNPLGSKVTPVAVNEILGLNTLGISLARVDFAPKGLNPPHTHPRGTEILVVLEGTLYVGFVTSNQDNNRLFTKVLNKGDVFVFPIGLIHFQLNVGYGEAIAIAGLSSQNPGVITIANAVFGSKPPISLEVLTKAFQVDKNVIDYLQKQFWYNNS