AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene037519

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037519.t1 MTR_5g046410 98.198 222 3 1 1 222 1 221 5.44e-158 436
MS.gene037519.t1 MTR_5g046430 98.198 222 3 1 1 222 1 221 5.44e-158 436
MS.gene037519.t1 MTR_4g017030 98.198 222 3 1 1 222 1 221 5.44e-158 436
MS.gene037519.t1 MTR_4g017050 97.748 222 4 1 1 222 1 221 1.90e-157 435
MS.gene037519.t1 MTR_2g019250 95.946 222 8 1 1 222 1 221 9.14e-156 431
MS.gene037519.t1 MTR_4g017040 96.396 222 7 1 1 222 1 221 9.24e-156 431
MS.gene037519.t1 MTR_1g079490 79.821 223 43 2 1 222 1 222 6.11e-127 358
MS.gene037519.t1 MTR_6g005340 77.130 223 47 3 1 222 1 220 3.97e-118 335
MS.gene037519.t1 MTR_6g005360 75.225 222 53 1 1 222 1 220 4.38e-118 335
MS.gene037519.t1 MTR_6g005330 76.682 223 48 3 1 222 1 220 4.88e-118 335
MS.gene037519.t1 MTR_6g005310 77.027 222 47 3 1 221 1 219 1.08e-117 334
MS.gene037519.t1 MTR_6g005350 62.613 222 80 2 1 222 1 219 1.62e-91 268
MS.gene037519.t1 MTR_6g005380 61.244 209 80 1 1 209 1 208 1.12e-87 258
MS.gene037519.t1 MTR_1g023770 59.174 218 77 3 1 216 1 208 8.39e-81 240
MS.gene037519.t1 MTR_2g030855 60.199 201 69 4 19 216 19 211 4.55e-77 231
MS.gene037519.t1 MTR_2g031270 57.273 220 82 5 1 216 1 212 4.61e-73 221
MS.gene037519.t1 MTR_2g030895 63.793 174 56 4 47 216 7 177 1.45e-70 213
MS.gene037519.t1 MTR_2g030865 63.218 174 57 4 47 216 7 177 1.22e-68 208
MS.gene037519.t1 MTR_1g085500 46.296 216 108 3 3 216 4 213 6.14e-61 190
MS.gene037519.t1 MTR_1g085140 46.269 201 100 3 18 216 20 214 3.35e-54 173
MS.gene037519.t1 MTR_7g111240 47.716 197 95 3 22 216 20 210 1.42e-52 169
MS.gene037519.t1 MTR_7g111240 47.716 197 92 3 22 216 20 207 2.57e-51 165
MS.gene037519.t1 MTR_2g031300 54.070 172 53 4 47 215 7 155 5.37e-51 162
MS.gene037519.t1 MTR_5g036570 45.455 198 99 4 22 216 27 218 9.58e-49 159
MS.gene037519.t1 MTR_1g112200 41.500 200 102 5 19 216 20 206 3.90e-44 147
MS.gene037519.t1 MTR_1g060120 36.036 222 129 5 2 216 5 220 3.01e-41 140
MS.gene037519.t1 MTR_2g041550 41.315 213 107 5 4 214 7 203 4.97e-41 139
MS.gene037519.t1 MTR_2g041570 38.605 215 114 5 3 215 6 204 1.92e-39 135
MS.gene037519.t1 MTR_2g041580 38.785 214 113 5 3 214 6 203 1.97e-39 135
MS.gene037519.t1 MTR_8g007300 40.299 201 106 4 22 220 26 214 1.07e-36 128
MS.gene037519.t1 MTR_8g020750 40.404 198 97 5 1 191 1 184 6.20e-35 123
MS.gene037519.t1 MTR_3g019710 39.691 194 106 5 7 198 3 187 2.61e-33 119
MS.gene037519.t1 MTR_8g020590 37.788 217 118 6 1 215 1 202 3.82e-33 119
MS.gene037519.t1 MTR_8g020630 39.205 176 97 4 7 180 3 170 7.87e-33 118
MS.gene037519.t1 MTR_2g036120 37.264 212 116 6 1 207 1 200 5.93e-31 113
MS.gene037519.t1 MTR_8g020650 36.269 193 111 5 26 216 22 204 9.10e-31 112
MS.gene037519.t1 MTR_8g020770 41.111 180 93 4 1 177 1 170 1.13e-30 112
MS.gene037519.t1 MTR_8g020670 34.419 215 123 6 4 216 6 204 1.20e-29 110
MS.gene037519.t1 MTR_3g019650 40.000 175 94 5 26 198 21 186 1.55e-29 109
MS.gene037519.t1 MTR_3g019680 37.563 197 105 6 4 198 6 186 1.76e-29 109
MS.gene037519.t1 MTR_0284s0010 36.893 206 117 5 7 207 3 200 4.24e-29 108
MS.gene037519.t1 MTR_2g044040 36.782 174 100 4 7 178 3 168 1.37e-28 107
MS.gene037519.t1 MTR_2g086640 33.945 218 117 7 2 215 7 201 3.84e-28 105
MS.gene037519.t1 MTR_2g086620 42.424 132 69 3 85 215 77 202 4.87e-28 105
MS.gene037519.t1 MTR_2g045100 41.290 155 81 4 26 178 22 168 5.07e-28 105
MS.gene037519.t1 MTR_4g052780 36.098 205 104 6 16 215 21 203 6.04e-28 105
MS.gene037519.t1 MTR_3g096330 38.333 180 104 3 3 180 558 732 6.66e-28 111
MS.gene037519.t1 MTR_4g052770 35.610 205 105 6 16 215 21 203 9.54e-28 105
MS.gene037519.t1 MTR_2g086630 41.667 132 70 3 85 215 77 202 1.49e-27 104
MS.gene037519.t1 MTR_5g031460 34.597 211 123 5 1 207 1 200 1.17e-26 102
MS.gene037519.t1 MTR_4g010340 35.000 220 118 7 1 216 2 200 2.32e-25 98.6
MS.gene037519.t1 MTR_8g020760 33.945 218 130 6 1 216 1 206 2.50e-25 99.0
MS.gene037519.t1 MTR_7g011340 37.433 187 104 5 26 207 22 200 3.73e-25 98.2
MS.gene037519.t1 MTR_8g020763 36.471 170 98 3 43 207 17 181 2.19e-24 95.9
MS.gene037519.t1 MTR_2g019780 32.727 220 131 7 1 216 1 207 2.54e-24 96.3
MS.gene037519.t1 MTR_3g073170 36.022 186 102 6 28 207 27 201 6.62e-24 95.1
MS.gene037519.t1 MTR_8g020610 33.333 216 126 7 1 214 1 200 9.14e-24 94.7
MS.gene037519.t1 MTR_2g030915 49.351 77 23 1 93 169 1 61 3.42e-18 77.0
MS.gene037519.t1 MTR_3g019810 33.495 206 114 9 1 203 107 292 1.58e-17 79.7
MS.gene037519.t1 MTR_4g027415 37.984 129 70 4 26 152 22 142 2.64e-16 73.2
MS.gene037519.t1 MTR_2g072560 36.066 122 69 4 1 120 1 115 1.81e-12 63.2
MS.gene037519.t1 MTR_3g019610 37.736 106 61 3 7 110 3 105 9.15e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037519.t1 AT3G05950 65.044 226 70 5 4 222 5 228 3.03e-95 278
MS.gene037519.t1 AT5G39150 64.151 212 72 3 7 216 9 218 2.09e-92 270
MS.gene037519.t1 AT5G39180 64.151 212 72 3 7 216 9 218 3.68e-92 270
MS.gene037519.t1 AT5G39120 64.151 212 72 3 7 216 9 218 3.77e-92 270
MS.gene037519.t1 AT5G39160 65.238 210 69 3 9 216 11 218 1.44e-90 266
MS.gene037519.t1 AT5G39110 63.679 212 74 2 7 216 9 219 1.52e-90 265
MS.gene037519.t1 AT5G39190 65.238 210 69 3 9 216 11 218 2.06e-90 265
MS.gene037519.t1 AT5G39130 64.286 210 71 3 9 216 11 218 6.72e-90 264
MS.gene037519.t1 AT4G14630 60.000 220 83 3 1 216 3 221 3.79e-86 254
MS.gene037519.t1 AT5G39160 62.857 210 70 3 9 216 11 214 1.26e-85 253
MS.gene037519.t1 AT3G04200 61.722 209 76 4 8 214 13 219 4.33e-82 244
MS.gene037519.t1 AT5G38910 59.729 221 81 4 1 216 1 218 7.66e-80 238
MS.gene037519.t1 AT5G39190 59.135 208 63 2 9 216 11 196 3.27e-78 233
MS.gene037519.t1 AT5G38940 60.914 197 73 2 22 216 26 220 4.45e-78 234
MS.gene037519.t1 AT5G38930 61.809 199 72 2 20 216 24 220 5.25e-78 234
MS.gene037519.t1 AT5G38940 60.914 197 73 2 22 216 50 244 7.30e-78 234
MS.gene037519.t1 AT5G39160 58.654 208 64 2 9 216 11 196 7.34e-78 233
MS.gene037519.t1 AT5G38960 59.901 202 76 3 18 216 22 221 1.19e-77 233
MS.gene037519.t1 AT3G04170 55.238 210 87 4 8 215 12 216 9.04e-73 221
MS.gene037519.t1 AT3G04190 55.446 202 86 3 15 214 19 218 3.36e-72 219
MS.gene037519.t1 AT3G04150 53.917 217 93 4 4 215 5 219 4.11e-72 219
MS.gene037519.t1 AT3G04150 51.092 229 94 4 4 215 5 232 4.22e-70 214
MS.gene037519.t1 AT3G04180 53.960 202 89 3 15 214 19 218 3.85e-69 211
MS.gene037519.t1 AT1G02335 43.721 215 113 3 4 216 6 214 1.20e-56 179
MS.gene037519.t1 AT5G38910 48.416 221 69 4 1 216 1 181 8.52e-56 176
MS.gene037519.t1 AT3G62020 46.262 214 105 5 6 216 8 214 2.06e-55 176
MS.gene037519.t1 AT1G09560 45.545 202 102 3 17 216 19 214 5.66e-54 172
MS.gene037519.t1 AT3G05930 47.783 203 96 4 4 203 6 201 2.53e-53 171
MS.gene037519.t1 AT5G26700 49.457 184 78 5 26 205 25 197 3.79e-51 165
MS.gene037519.t1 AT1G18970 44.776 201 101 4 18 216 25 217 1.94e-49 161
MS.gene037519.t1 AT1G18980 45.274 201 100 4 18 216 25 217 3.46e-48 158
MS.gene037519.t1 AT3G62020 46.961 181 89 3 39 216 9 185 6.56e-48 156
MS.gene037519.t1 AT1G10460 41.709 199 107 5 22 215 19 213 3.41e-45 150
MS.gene037519.t1 AT3G10080 39.409 203 113 5 18 216 22 218 2.19e-43 145
MS.gene037519.t1 AT3G10080 39.409 203 113 5 18 216 93 289 9.21e-43 146
MS.gene037519.t1 AT5G61750 41.398 186 103 2 4 187 1 182 1.26e-40 138
MS.gene037519.t1 AT5G20630 41.232 211 111 6 1 209 1 200 2.13e-40 137
MS.gene037519.t1 AT1G74820 38.660 194 109 4 25 216 39 224 2.34e-32 117
MS.gene037519.t1 AT1G72610 39.791 191 105 6 26 214 19 201 2.34e-32 117
MS.gene037519.t1 AT5G38950 54.167 72 33 0 146 217 2 73 1.24e-18 78.2
MS.gene037519.t1 AT5G39100 72.000 50 14 0 7 56 9 58 7.69e-18 74.7

Find 54 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATATACATACCACCATCTT+TGG 0.255130 4.3:-82514842 None:intergenic
TTTCAACTGAATGTTGGATA+TGG 0.274546 4.3:+82515418 MS.gene037519:CDS
TAACACTCTTGGTATATCTT+TGG 0.286436 4.3:+82515204 MS.gene037519:CDS
CCTTGTTAGCATCTTGGCTT+TGG 0.311083 4.3:+82514753 MS.gene037519:CDS
TTGAGAGCCTAGTGCGTTAG+AGG 0.314204 4.3:-82515146 None:intergenic
CTTATCCACTTGAAAAGCTT+TGG 0.335788 4.3:-82515545 None:intergenic
TGAAAATGAATAAGACCAAT+TGG 0.356438 4.3:-82515400 None:intergenic
GAGTGCCTTCAAGTACTATA+AGG 0.386700 4.3:-82515291 None:intergenic
ATACTTCCTTGTTAGCATCT+TGG 0.401215 4.3:+82514747 MS.gene037519:CDS
TTCTTCAAACATGTTGAAGC+TGG 0.402098 4.3:+82515118 MS.gene037519:CDS
ATATACATACCACCATCTTT+GGG 0.405165 4.3:-82514841 None:intergenic
CTCTAGGGTGGATGTGAGGT+GGG 0.411548 4.3:-82515258 None:intergenic
GATGTGTTTGTGTTCCCAAT+TGG 0.424230 4.3:+82515385 MS.gene037519:CDS
GGCAACGCGGTTGCTATCGC+TGG 0.429748 4.3:+82515439 MS.gene037519:CDS
CAAAAGTCTTGAAGAGGGCT+GGG 0.439382 4.3:-82514802 None:intergenic
ATTATCTTCAAAAGCAGTCT+TGG 0.442184 4.3:+82515578 MS.gene037519:CDS
TCTTCAGCTTTAACAAGTGC+GGG 0.444792 4.3:-82515094 None:intergenic
ACAAAAGTCTTGAAGAGGGC+TGG 0.445972 4.3:-82514803 None:intergenic
GTCACTGTAGACCAACTATT+CGG 0.446134 4.3:+82515178 MS.gene037519:CDS
CTTGAAGGCACTCTTTATGT+TGG 0.454430 4.3:+82515301 MS.gene037519:CDS
TACTTTGCAGTATTTGTGAA+TGG 0.463825 4.3:+82515055 MS.gene037519:intron
ATAAGGATCTCTGTTCCTCT+AGG 0.468428 4.3:-82515274 None:intergenic
GAGATCCTTATAGTACTTGA+AGG 0.469573 4.3:+82515286 MS.gene037519:CDS
TTTGCAACGGTGATAACTCC+TGG 0.470922 4.3:-82515478 None:intergenic
CTATTCGGACTTAACACTCT+TGG 0.480493 4.3:+82515193 MS.gene037519:CDS
CCTCTAGGGTGGATGTGAGG+TGG 0.496940 4.3:-82515259 None:intergenic
TCTTCAAACATGTTGAAGCT+GGG 0.501315 4.3:+82515119 MS.gene037519:CDS
GAAAATGAATAAGACCAATT+GGG 0.510497 4.3:-82515399 None:intergenic
AGAACTTCGTCGGAAATAAG+AGG 0.511108 4.3:-82515520 None:intergenic
GAGTGTTAAGTCCGAATAGT+TGG 0.535207 4.3:-82515189 None:intergenic
TGAGGTGGGTTTAAACCCTT+AGG 0.537084 4.3:-82515244 None:intergenic
ATCTTCAGCTTTAACAAGTG+CGG 0.543612 4.3:-82515095 None:intergenic
CTTTACCAAGGTGCTCAACA+AGG 0.544491 4.3:+82515360 MS.gene037519:CDS
GCAATCAAAGACCCCAAAGA+TGG 0.554146 4.3:+82514829 MS.gene037519:CDS
AGCTTTGGTAAGAACTTCGT+CGG 0.555884 4.3:-82515530 None:intergenic
CCAAAGCCAAGATGCTAACA+AGG 0.556137 4.3:-82514753 None:intergenic
ATCACCCTTGTTGAGCACCT+TGG 0.567836 4.3:-82515365 None:intergenic
TAAGGATCTCTGTTCCTCTA+GGG 0.571738 4.3:-82515273 None:intergenic
GGGAATGCCTCTAACGCACT+AGG 0.573570 4.3:+82515139 MS.gene037519:CDS
GGACTTAGCAGTCAAAATCC+AGG 0.578792 4.3:+82515460 MS.gene037519:CDS
GGATCTCTGTTCCTCTAGGG+TGG 0.590427 4.3:-82515270 None:intergenic
TTTAAACAAAGCATTTGCAA+CGG 0.590900 4.3:-82515491 None:intergenic
GCAACACAAAAGTCTTGAAG+AGG 0.609121 4.3:-82514808 None:intergenic
AAAAGTCTTGAAGAGGGCTG+GGG 0.612013 4.3:-82514801 None:intergenic
ATCAAAGACCCCAAAGATGG+TGG 0.628410 4.3:+82514832 MS.gene037519:CDS
AGGGCTGGGGTCATAAGCAA+AGG 0.635001 4.3:-82514788 None:intergenic
TATACATACCACCATCTTTG+GGG 0.639620 4.3:-82514840 None:intergenic
GTTCCTCTAGGGTGGATGTG+AGG 0.650118 4.3:-82515262 None:intergenic
CAACACAAAAGTCTTGAAGA+GGG 0.650648 4.3:-82514807 None:intergenic
TTTACCAAGGTGCTCAACAA+GGG 0.659466 4.3:+82515361 MS.gene037519:CDS
CCACCTCACATCCACCCTAG+AGG 0.660499 4.3:+82515259 MS.gene037519:CDS
CTTCAGCTTTAACAAGTGCG+GGG 0.662073 4.3:-82515093 None:intergenic
CAATAATCGTCTCTTTACCA+AGG 0.672342 4.3:+82515348 MS.gene037519:CDS
GAATGTTGGATATGGCAACG+CGG 0.840401 4.3:+82515426 MS.gene037519:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAATAAAAAATTTAT+AGG - chr4.3:82514958-82514977 None:intergenic 0.0%
!! AAAAAAATAAAAAATTTATA+GGG - chr4.3:82514957-82514976 None:intergenic 0.0%
!!! ATAAATTTTTTATTTTTTTT+TGG + chr4.3:82514958-82514977 MS.gene037519:intron 0.0%
!!! ATATATAGAATCTTTTGTTT+TGG + chr4.3:82514994-82515013 MS.gene037519:intron 15.0%
!! CAAAAGATTCTATATATACA+TGG - chr4.3:82514991-82515010 None:intergenic 20.0%
!! GTAATCAAGAATTAAAGTTA+AGG - chr4.3:82514870-82514889 None:intergenic 20.0%
!! TCTACAAATATTAGACTTAT+TGG + chr4.3:82514924-82514943 MS.gene037519:intron 20.0%
! AATCATAGTTTAGTTAAACC+AGG - chr4.3:82515032-82515051 None:intergenic 25.0%
! GAAAATGAATAAGACCAATT+GGG - chr4.3:82515402-82515421 None:intergenic 25.0%
! TGAAAATGAATAAGACCAAT+TGG - chr4.3:82515403-82515422 None:intergenic 25.0%
!! ATTCATTTTCAACTGAATGT+TGG + chr4.3:82515412-82515431 MS.gene037519:CDS 25.0%
!! TTTAAACAAAGCATTTGCAA+CGG - chr4.3:82515494-82515513 None:intergenic 25.0%
ATATACATACCACCATCTTT+GGG - chr4.3:82514844-82514863 None:intergenic 30.0%
ATTATCTTCAAAAGCAGTCT+TGG + chr4.3:82515578-82515597 MS.gene037519:CDS 30.0%
TAACACTCTTGGTATATCTT+TGG + chr4.3:82515204-82515223 MS.gene037519:CDS 30.0%
TACTTTGCAGTATTTGTGAA+TGG + chr4.3:82515055-82515074 MS.gene037519:intron 30.0%
TATATACATACCACCATCTT+TGG - chr4.3:82514845-82514864 None:intergenic 30.0%
TTTCAACTGAATGTTGGATA+TGG + chr4.3:82515418-82515437 MS.gene037519:CDS 30.0%
!!! GAATCTTTTGTTTTGGTTCA+TGG + chr4.3:82515001-82515020 MS.gene037519:intron 30.0%
ATACTTCCTTGTTAGCATCT+TGG + chr4.3:82514747-82514766 MS.gene037519:CDS 35.0%
CAACACAAAAGTCTTGAAGA+GGG - chr4.3:82514810-82514829 None:intergenic 35.0%
CAATAATCGTCTCTTTACCA+AGG + chr4.3:82515348-82515367 MS.gene037519:CDS 35.0%
CTTATCCACTTGAAAAGCTT+TGG - chr4.3:82515548-82515567 None:intergenic 35.0%
GAGATCCTTATAGTACTTGA+AGG + chr4.3:82515286-82515305 MS.gene037519:CDS 35.0%
TATACATACCACCATCTTTG+GGG - chr4.3:82514843-82514862 None:intergenic 35.0%
TCTTCAAACATGTTGAAGCT+GGG + chr4.3:82515119-82515138 MS.gene037519:CDS 35.0%
TTCTTCAAACATGTTGAAGC+TGG + chr4.3:82515118-82515137 MS.gene037519:CDS 35.0%
! ATCTTCAGCTTTAACAAGTG+CGG - chr4.3:82515098-82515117 None:intergenic 35.0%
!! TCTTACCAAAGCTTTTCAAG+TGG + chr4.3:82515540-82515559 MS.gene037519:CDS 35.0%
AGAACTTCGTCGGAAATAAG+AGG - chr4.3:82515523-82515542 None:intergenic 40.0%
ATAAGGATCTCTGTTCCTCT+AGG - chr4.3:82515277-82515296 None:intergenic 40.0%
CTATTCGGACTTAACACTCT+TGG + chr4.3:82515193-82515212 MS.gene037519:CDS 40.0%
GAGTGCCTTCAAGTACTATA+AGG - chr4.3:82515294-82515313 None:intergenic 40.0%
GAGTGTTAAGTCCGAATAGT+TGG - chr4.3:82515192-82515211 None:intergenic 40.0%
GATGTGTTTGTGTTCCCAAT+TGG + chr4.3:82515385-82515404 MS.gene037519:CDS 40.0%
GCAACACAAAAGTCTTGAAG+AGG - chr4.3:82514811-82514830 None:intergenic 40.0%
GTCACTGTAGACCAACTATT+CGG + chr4.3:82515178-82515197 MS.gene037519:CDS 40.0%
TAAGGATCTCTGTTCCTCTA+GGG - chr4.3:82515276-82515295 None:intergenic 40.0%
! AGCTTTGGTAAGAACTTCGT+CGG - chr4.3:82515533-82515552 None:intergenic 40.0%
! TCTTCAGCTTTAACAAGTGC+GGG - chr4.3:82515097-82515116 None:intergenic 40.0%
! TTTACCAAGGTGCTCAACAA+GGG + chr4.3:82515361-82515380 MS.gene037519:CDS 40.0%
!! CTTGAAGGCACTCTTTATGT+TGG + chr4.3:82515301-82515320 MS.gene037519:CDS 40.0%
!!! TTTTGGTTCATGGTGTTTCC+TGG + chr4.3:82515011-82515030 MS.gene037519:intron 40.0%
AAAAGTCTTGAAGAGGGCTG+GGG - chr4.3:82514804-82514823 None:intergenic 45.0%
ACAAAAGTCTTGAAGAGGGC+TGG - chr4.3:82514806-82514825 None:intergenic 45.0%
ATCAAAGACCCCAAAGATGG+TGG + chr4.3:82514832-82514851 MS.gene037519:CDS 45.0%
CAAAAGTCTTGAAGAGGGCT+GGG - chr4.3:82514805-82514824 None:intergenic 45.0%
CCAAAGCCAAGATGCTAACA+AGG - chr4.3:82514756-82514775 None:intergenic 45.0%
CCTTGTTAGCATCTTGGCTT+TGG + chr4.3:82514753-82514772 MS.gene037519:CDS 45.0%
GAATGTTGGATATGGCAACG+CGG + chr4.3:82515426-82515445 MS.gene037519:CDS 45.0%
GCAATCAAAGACCCCAAAGA+TGG + chr4.3:82514829-82514848 MS.gene037519:CDS 45.0%
GGACTTAGCAGTCAAAATCC+AGG + chr4.3:82515460-82515479 MS.gene037519:CDS 45.0%
TTTGCAACGGTGATAACTCC+TGG - chr4.3:82515481-82515500 None:intergenic 45.0%
! CTTCAGCTTTAACAAGTGCG+GGG - chr4.3:82515096-82515115 None:intergenic 45.0%
! CTTTACCAAGGTGCTCAACA+AGG + chr4.3:82515360-82515379 MS.gene037519:CDS 45.0%
! TGAGGTGGGTTTAAACCCTT+AGG - chr4.3:82515247-82515266 None:intergenic 45.0%
ATCACCCTTGTTGAGCACCT+TGG - chr4.3:82515368-82515387 None:intergenic 50.0%
TTGAGAGCCTAGTGCGTTAG+AGG - chr4.3:82515149-82515168 None:intergenic 50.0%
!!! CGCGTTGATTTTGCGCCTAA+GGG + chr4.3:82515229-82515248 MS.gene037519:CDS 50.0%
!!! TCGCGTTGATTTTGCGCCTA+AGG + chr4.3:82515228-82515247 MS.gene037519:CDS 50.0%
AGGGCTGGGGTCATAAGCAA+AGG - chr4.3:82514791-82514810 None:intergenic 55.0%
GGGAATGCCTCTAACGCACT+AGG + chr4.3:82515139-82515158 MS.gene037519:CDS 55.0%
! GGATCTCTGTTCCTCTAGGG+TGG - chr4.3:82515273-82515292 None:intergenic 55.0%
!! CTCTAGGGTGGATGTGAGGT+GGG - chr4.3:82515261-82515280 None:intergenic 55.0%
!! GTTCCTCTAGGGTGGATGTG+AGG - chr4.3:82515265-82515284 None:intergenic 55.0%
CCACCTCACATCCACCCTAG+AGG + chr4.3:82515259-82515278 MS.gene037519:CDS 60.0%
!! CCTCTAGGGTGGATGTGAGG+TGG - chr4.3:82515262-82515281 None:intergenic 60.0%
GGCAACGCGGTTGCTATCGC+TGG + chr4.3:82515439-82515458 MS.gene037519:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 82514736 82515615 82514736 ID=MS.gene037519
chr4.3 mRNA 82514736 82515615 82514736 ID=MS.gene037519.t1;Parent=MS.gene037519
chr4.3 exon 82514736 82514853 82514736 ID=MS.gene037519.t1.exon1;Parent=MS.gene037519.t1
chr4.3 CDS 82514736 82514853 82514736 ID=cds.MS.gene037519.t1;Parent=MS.gene037519.t1
chr4.3 exon 82515065 82515615 82515065 ID=MS.gene037519.t1.exon2;Parent=MS.gene037519.t1
chr4.3 CDS 82515065 82515615 82515065 ID=cds.MS.gene037519.t1;Parent=MS.gene037519.t1
Gene Sequence

>MS.gene037519

ATGAAGACTATATACTTCCTTGTTAGCATCTTGGCTTTGGCATCATCTGTTGCCTTTGCTTATGACCCCAGCCCTCTTCAAGACTTTTGTGTTGCAATCAAAGACCCCAAAGATGGTGGTATGTATATATTCCTTAACTTTAATTCTTGATTACTACTCAATTTTTAAGCTAACATATGAAAATGTTTTCTACAAATATTAGACTTATTGGTTTTAATCCCTATAAATTTTTTATTTTTTTTTGGTTTTAGTCCATGTATATATAGAATCTTTTGTTTTGGTTCATGGTGTTTCCTGGTTTAACTAAACTATGATTTGATACTTTGCAGTATTTGTGAATGGAAAATTCTGTAAAGACCCCGCACTTGTTAAAGCTGAAGATTTCTTCAAACATGTTGAAGCTGGGAATGCCTCTAACGCACTAGGCTCTCAAGTAACTCAAGTCACTGTAGACCAACTATTCGGACTTAACACTCTTGGTATATCTTTGGCTCGCGTTGATTTTGCGCCTAAGGGTTTAAACCCACCTCACATCCACCCTAGAGGAACAGAGATCCTTATAGTACTTGAAGGCACTCTTTATGTTGGATTTGTCACGTCAAATCAAGACAACAATAATCGTCTCTTTACCAAGGTGCTCAACAAGGGTGATGTGTTTGTGTTCCCAATTGGTCTTATTCATTTTCAACTGAATGTTGGATATGGCAACGCGGTTGCTATCGCTGGACTTAGCAGTCAAAATCCAGGAGTTATCACCGTTGCAAATGCTTTGTTTAAATCTGATCCTCTTATTTCCGACGAAGTTCTTACCAAAGCTTTTCAAGTGGATAAGAGCATAATTGATTATCTTCAAAAGCAGTCTTGGTATGACAACAACTAG
Protein sequence

>MS.gene037519.t1

MKTIYFLVSILALASSVAFAYDPSPLQDFCVAIKDPKDGVFVNGKFCKDPALVKAEDFFKHVEAGNASNALGSQVTQVTVDQLFGLNTLGISLARVDFAPKGLNPPHIHPRGTEILIVLEGTLYVGFVTSNQDNNNRLFTKVLNKGDVFVFPIGLIHFQLNVGYGNAVAIAGLSSQNPGVITVANALFKSDPLISDEVLTKAFQVDKSIIDYLQKQSWYDNN