AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene037520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037520.t1 MTR_5g046410 97.748 222 4 1 1 222 1 221 1.53e-157 435
MS.gene037520.t1 MTR_5g046430 97.748 222 4 1 1 222 1 221 1.53e-157 435
MS.gene037520.t1 MTR_4g017030 97.748 222 4 1 1 222 1 221 1.53e-157 435
MS.gene037520.t1 MTR_4g017050 97.297 222 5 1 1 222 1 221 5.51e-157 434
MS.gene037520.t1 MTR_2g019250 95.946 222 8 1 1 222 1 221 1.24e-155 430
MS.gene037520.t1 MTR_4g017040 95.946 222 8 1 1 222 1 221 3.16e-155 429
MS.gene037520.t1 MTR_1g079490 78.924 223 45 2 1 222 1 222 3.16e-126 356
MS.gene037520.t1 MTR_6g005340 76.256 219 49 2 4 222 5 220 1.65e-116 331
MS.gene037520.t1 MTR_6g005330 75.785 223 50 3 1 222 1 220 2.34e-116 331
MS.gene037520.t1 MTR_6g005360 73.874 222 56 1 1 222 1 220 2.72e-116 330
MS.gene037520.t1 MTR_6g005310 76.147 218 49 2 4 221 5 219 7.30e-116 330
MS.gene037520.t1 MTR_6g005350 62.727 220 78 3 3 222 4 219 9.70e-90 263
MS.gene037520.t1 MTR_6g005380 60.287 209 82 1 1 209 1 208 4.37e-86 254
MS.gene037520.t1 MTR_1g023770 58.257 218 79 3 1 216 1 208 3.26e-79 236
MS.gene037520.t1 MTR_2g030855 59.204 201 71 4 19 216 19 211 6.12e-76 228
MS.gene037520.t1 MTR_2g031270 58.416 202 72 5 19 216 19 212 1.23e-71 217
MS.gene037520.t1 MTR_2g030895 62.644 174 58 4 47 216 7 177 1.84e-69 210
MS.gene037520.t1 MTR_2g030865 62.069 174 59 4 47 216 7 177 1.66e-67 205
MS.gene037520.t1 MTR_1g085500 46.262 214 107 3 5 216 6 213 2.52e-61 191
MS.gene037520.t1 MTR_1g085140 45.771 201 101 3 18 216 20 214 9.30e-54 172
MS.gene037520.t1 MTR_7g111240 47.208 197 96 3 22 216 20 210 2.52e-52 168
MS.gene037520.t1 MTR_7g111240 47.208 197 93 3 22 216 20 207 3.88e-51 165
MS.gene037520.t1 MTR_2g031300 52.907 172 55 4 47 215 7 155 1.12e-49 159
MS.gene037520.t1 MTR_5g036570 44.949 198 100 4 22 216 27 218 4.61e-48 157
MS.gene037520.t1 MTR_1g112200 41.500 200 102 5 19 216 20 206 1.31e-44 148
MS.gene037520.t1 MTR_1g060120 36.486 222 128 5 2 216 5 220 3.25e-41 140
MS.gene037520.t1 MTR_2g041550 40.376 213 109 5 4 214 7 203 3.94e-40 137
MS.gene037520.t1 MTR_2g041570 37.383 214 116 5 4 215 7 204 1.07e-37 130
MS.gene037520.t1 MTR_2g041580 37.559 213 115 5 4 214 7 203 1.27e-37 130
MS.gene037520.t1 MTR_8g007300 40.299 201 106 4 22 220 26 214 6.04e-37 129
MS.gene037520.t1 MTR_8g020750 40.404 198 97 5 1 191 1 184 3.91e-35 124
MS.gene037520.t1 MTR_3g019710 41.000 200 103 6 1 198 1 187 1.62e-34 122
MS.gene037520.t1 MTR_8g020590 36.866 217 119 6 1 215 2 202 3.55e-33 119
MS.gene037520.t1 MTR_8g020630 40.217 184 92 6 1 180 1 170 4.76e-33 119
MS.gene037520.t1 MTR_2g036120 37.736 212 115 6 1 207 1 200 1.03e-32 117
MS.gene037520.t1 MTR_8g020770 41.111 180 93 4 1 177 1 170 6.82e-31 113
MS.gene037520.t1 MTR_3g019680 38.119 202 105 7 1 198 1 186 1.22e-30 112
MS.gene037520.t1 MTR_3g019650 38.119 202 105 7 1 198 1 186 1.33e-30 112
MS.gene037520.t1 MTR_8g020650 34.862 218 126 6 1 216 1 204 1.88e-30 112
MS.gene037520.t1 MTR_2g044040 37.778 180 98 5 1 178 1 168 3.85e-30 111
MS.gene037520.t1 MTR_8g020670 35.000 220 123 7 1 216 1 204 4.47e-30 111
MS.gene037520.t1 MTR_2g086640 34.862 218 115 7 2 215 7 201 8.17e-30 110
MS.gene037520.t1 MTR_0284s0010 37.736 212 115 6 1 207 1 200 1.06e-29 110
MS.gene037520.t1 MTR_4g052780 37.073 205 102 6 16 215 21 203 1.35e-29 109
MS.gene037520.t1 MTR_2g086620 43.939 132 67 3 85 215 77 202 2.62e-29 108
MS.gene037520.t1 MTR_2g045100 38.889 180 96 5 1 178 1 168 3.43e-29 108
MS.gene037520.t1 MTR_4g052770 34.862 218 114 7 3 215 9 203 3.55e-29 108
MS.gene037520.t1 MTR_2g086630 43.182 132 68 3 85 215 77 202 7.54e-29 107
MS.gene037520.t1 MTR_3g096330 39.326 178 101 3 5 180 560 732 2.21e-28 112
MS.gene037520.t1 MTR_5g031460 35.071 211 122 5 1 207 1 200 3.42e-28 106
MS.gene037520.t1 MTR_8g020760 34.404 218 129 6 1 216 1 206 5.51e-26 100
MS.gene037520.t1 MTR_7g011340 35.849 212 119 6 1 207 1 200 3.97e-25 98.2
MS.gene037520.t1 MTR_4g010340 35.321 218 115 8 3 216 5 200 5.96e-25 97.8
MS.gene037520.t1 MTR_8g020610 33.333 216 126 7 1 214 1 200 8.22e-25 97.4
MS.gene037520.t1 MTR_2g019780 32.727 220 131 7 1 216 1 207 1.06e-24 97.1
MS.gene037520.t1 MTR_8g020763 35.795 176 104 3 43 216 17 185 2.82e-24 95.5
MS.gene037520.t1 MTR_3g073170 34.715 193 110 6 27 216 26 205 4.34e-24 95.5
MS.gene037520.t1 MTR_3g019810 33.981 206 113 9 1 203 107 292 1.52e-18 82.8
MS.gene037520.t1 MTR_2g030915 46.753 77 25 1 93 169 1 61 7.81e-17 73.6
MS.gene037520.t1 MTR_4g027415 35.714 154 85 5 1 152 1 142 1.99e-16 73.6
MS.gene037520.t1 MTR_2g072560 36.885 122 68 4 1 120 1 115 6.73e-14 67.0
MS.gene037520.t1 MTR_3g019610 38.393 112 60 4 1 110 1 105 1.17e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037520.t1 AT3G05950 64.159 226 72 5 4 222 5 228 5.88e-94 275
MS.gene037520.t1 AT5G39150 62.100 219 79 3 1 216 1 218 1.59e-91 268
MS.gene037520.t1 AT5G39180 62.100 219 79 3 1 216 1 218 3.58e-91 267
MS.gene037520.t1 AT5G39120 63.208 212 74 3 7 216 9 218 4.36e-91 267
MS.gene037520.t1 AT5G39110 62.736 212 76 2 7 216 9 219 2.05e-89 263
MS.gene037520.t1 AT5G39160 62.727 220 76 4 1 216 1 218 2.14e-89 263
MS.gene037520.t1 AT5G39190 62.727 220 76 4 1 216 1 218 3.74e-89 262
MS.gene037520.t1 AT5G39130 63.333 210 73 3 9 216 11 218 8.97e-89 261
MS.gene037520.t1 AT5G39160 60.455 220 77 4 1 216 1 214 1.45e-84 250
MS.gene037520.t1 AT4G14630 59.091 220 85 3 1 216 3 221 1.52e-84 250
MS.gene037520.t1 AT3G04200 59.259 216 84 4 1 214 6 219 6.63e-82 244
MS.gene037520.t1 AT5G38910 59.276 221 82 4 1 216 1 218 1.38e-78 235
MS.gene037520.t1 AT5G38930 60.804 199 74 2 20 216 24 220 4.61e-77 231
MS.gene037520.t1 AT5G39190 56.881 218 70 3 1 216 1 196 4.97e-77 230
MS.gene037520.t1 AT5G38940 59.898 197 75 2 22 216 26 220 5.08e-77 231
MS.gene037520.t1 AT5G38940 59.898 197 75 2 22 216 50 244 7.62e-77 232
MS.gene037520.t1 AT5G38960 59.406 202 77 3 18 216 22 221 7.95e-77 231
MS.gene037520.t1 AT5G39160 56.422 218 71 3 1 216 1 196 1.39e-76 229
MS.gene037520.t1 AT3G04190 54.950 202 87 3 15 214 19 218 1.24e-71 218
MS.gene037520.t1 AT3G04170 54.808 208 87 4 10 215 14 216 2.54e-71 217
MS.gene037520.t1 AT3G04150 52.752 218 96 4 3 215 4 219 7.39e-71 216
MS.gene037520.t1 AT3G04150 50.000 230 97 4 3 215 4 232 6.02e-69 211
MS.gene037520.t1 AT3G04180 53.465 202 90 3 15 214 19 218 1.49e-68 210
MS.gene037520.t1 AT1G02335 43.256 215 114 3 4 216 6 214 3.51e-56 178
MS.gene037520.t1 AT3G62020 45.794 214 106 5 6 216 8 214 4.21e-55 175
MS.gene037520.t1 AT5G38910 48.416 221 69 4 1 216 1 181 4.81e-55 174
MS.gene037520.t1 AT3G05930 47.549 204 97 4 3 203 5 201 9.02e-54 172
MS.gene037520.t1 AT1G09560 44.554 202 104 3 17 216 19 214 4.45e-53 170
MS.gene037520.t1 AT5G26700 48.913 184 79 5 26 205 25 197 8.82e-51 164
MS.gene037520.t1 AT1G18970 44.776 201 101 4 18 216 25 217 2.87e-49 160
MS.gene037520.t1 AT1G18980 45.274 201 100 4 18 216 25 217 4.89e-48 157
MS.gene037520.t1 AT3G62020 46.409 181 90 3 39 216 9 185 1.65e-47 155
MS.gene037520.t1 AT1G10460 41.709 199 107 5 22 215 19 213 1.02e-44 149
MS.gene037520.t1 AT3G10080 39.409 203 113 5 18 216 22 218 4.09e-43 145
MS.gene037520.t1 AT3G10080 39.409 203 113 5 18 216 93 289 2.47e-42 145
MS.gene037520.t1 AT5G61750 41.398 186 103 2 4 187 1 182 2.16e-40 137
MS.gene037520.t1 AT5G20630 40.284 211 113 6 1 209 1 200 9.33e-40 136
MS.gene037520.t1 AT1G72610 39.791 191 105 6 26 214 19 201 1.93e-32 117
MS.gene037520.t1 AT1G74820 38.462 195 110 4 24 216 38 224 3.55e-32 117
MS.gene037520.t1 AT5G38950 54.167 72 33 0 146 217 2 73 1.08e-18 78.6
MS.gene037520.t1 AT5G39100 72.000 50 14 0 7 56 9 58 7.94e-18 74.7

Find 55 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATATACATACCACCATCTT+TGG 0.255130 4.3:-82505438 None:intergenic
GTCACTGTAGACCAACTATT+TGG 0.287743 4.3:+82506626 MS.gene037520:CDS
TTTCAACTGAACGTGGGATA+TGG 0.292065 4.3:+82506866 MS.gene037520:CDS
CCTTGTTAGCATCTTGGCTT+TGG 0.311083 4.3:+82505349 MS.gene037520:CDS
TTGAGAGCCTAGTGCGTTAG+AGG 0.314204 4.3:-82506594 None:intergenic
ATTATCTTCAAAAGCAATCT+TGG 0.335628 4.3:+82507026 MS.gene037520:CDS
CTTATCCACTTGAAAAGCTT+TGG 0.335788 4.3:-82506993 None:intergenic
CAACACACTTGGGATATCTT+TGG 0.340651 4.3:+82506652 MS.gene037520:CDS
ACCATCTTTGGGGTCTTTGA+TGG 0.343346 4.3:-82505426 None:intergenic
TGAAAATGAATAAGACCAAT+TGG 0.356438 4.3:-82506848 None:intergenic
GACTGCCTTCAAGTACTATA+AGG 0.359669 4.3:-82506739 None:intergenic
ACGATTAGTGTTGTCTTGAT+TGG 0.382865 4.3:-82506783 None:intergenic
ATACTTCCTTGTTAGCATCT+TGG 0.401215 4.3:+82505343 MS.gene037520:CDS
TTCTTCAAACATGTTGAAGC+TGG 0.402098 4.3:+82506566 MS.gene037520:CDS
ATATACATACCACCATCTTT+GGG 0.405165 4.3:-82505437 None:intergenic
AATTTGCAGTGTTTGTGAAC+GGG 0.408992 4.3:+82506504 MS.gene037520:intron
GGCAACGCTGTTGCTATTGC+TGG 0.409649 4.3:+82506887 MS.gene037520:CDS
GATGTGTTTGTGTTCCCAAT+TGG 0.424230 4.3:+82506833 MS.gene037520:CDS
CAAAAGTCTTGGAGAGGGCT+AGG 0.434671 4.3:-82505398 None:intergenic
TTTGCAACTGTGATAACTCC+TGG 0.466515 4.3:-82506926 None:intergenic
CTATTTGGACTCAACACACT+TGG 0.473975 4.3:+82506641 MS.gene037520:CDS
ATAAGGATTTCCGTTCCTCT+AGG 0.478305 4.3:-82506722 None:intergenic
GAAATCCTTATAGTACTTGA+AGG 0.481512 4.3:+82506734 MS.gene037520:CDS
TCTTCAGCTTTAACTAGCGC+GGG 0.484814 4.3:-82506542 None:intergenic
CTTGAAGGCAGTCTTTATGT+TGG 0.489113 4.3:+82506749 MS.gene037520:CDS
ATCACCCTTGTTGAGCACTT+TGG 0.491510 4.3:-82506813 None:intergenic
CTCTAGGGTGAATGTGAGGT+GGG 0.498933 4.3:-82506706 None:intergenic
TGATGGCAACACAAAAGTCT+TGG 0.500181 4.3:-82505409 None:intergenic
TCTTCAAACATGTTGAAGCT+GGG 0.501315 4.3:+82506567 MS.gene037520:CDS
GAAAATGAATAAGACCAATT+GGG 0.510497 4.3:-82506847 None:intergenic
AGAACTTCGTCGGAAATAAG+AGG 0.511108 4.3:-82506968 None:intergenic
TGAGGTGGGTTTAAACCCTT+AGG 0.540358 4.3:-82506692 None:intergenic
ATCTTCAGCTTTAACTAGCG+CGG 0.543736 4.3:-82506543 None:intergenic
TAAGGATTTCCGTTCCTCTA+GGG 0.546102 4.3:-82506721 None:intergenic
TAATTTGCAGTGTTTGTGAA+CGG 0.551349 4.3:+82506503 MS.gene037520:intron
GCCATCAAAGACCCCAAAGA+TGG 0.552100 4.3:+82505425 MS.gene037520:CDS
AGCTTTGGTAAGAACTTCGT+CGG 0.555884 4.3:-82506978 None:intergenic
CCAAAGCCAAGATGCTAACA+AGG 0.556137 4.3:-82505349 None:intergenic
TATTTGGACTCAACACACTT+GGG 0.557339 4.3:+82506642 MS.gene037520:CDS
AAAAGTCTTGGAGAGGGCTA+GGG 0.564154 4.3:-82505397 None:intergenic
CCTCTAGGGTGAATGTGAGG+TGG 0.565620 4.3:-82506707 None:intergenic
GTGTGTTGAGTCCAAATAGT+TGG 0.570150 4.3:-82506637 None:intergenic
GGGAATGCCTCTAACGCACT+AGG 0.573570 4.3:+82506587 MS.gene037520:CDS
GGACTTAGCAGTCAAAATCC+AGG 0.578792 4.3:+82506908 MS.gene037520:CDS
CTTCACCAAAGTGCTCAACA+AGG 0.595909 4.3:+82506808 MS.gene037520:CDS
AGGGCTAGGGTCATAAGCAA+AGG 0.622370 4.3:-82505384 None:intergenic
TCACATTCACCCTAGAGGAA+CGG 0.635178 4.3:+82506712 MS.gene037520:CDS
ATCAAAGACCCCAAAGATGG+TGG 0.635231 4.3:+82505428 MS.gene037520:CDS
TATACATACCACCATCTTTG+GGG 0.639620 4.3:-82505436 None:intergenic
TTCACCAAAGTGCTCAACAA+GGG 0.645837 4.3:+82506809 MS.gene037520:CDS
CAACACAAAAGTCTTGGAGA+GGG 0.646061 4.3:-82505403 None:intergenic
GCAACACAAAAGTCTTGGAG+AGG 0.651085 4.3:-82505404 None:intergenic
CTTCAGCTTTAACTAGCGCG+GGG 0.662073 4.3:-82506541 None:intergenic
GTTCCTCTAGGGTGAATGTG+AGG 0.681575 4.3:-82506710 None:intergenic
CCACCTCACATTCACCCTAG+AGG 0.685266 4.3:+82506707 MS.gene037520:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAGAGAAAATTTATA+TGG - chr4.3:82506363-82506382 None:intergenic 10.0%
!! ATATTTAGTATTATAGTATA+TGG + chr4.3:82506294-82506313 MS.gene037520:intron 10.0%
!! ATTAAAATGATGATTTAATT+TGG - chr4.3:82505808-82505827 None:intergenic 10.0%
!!! TATTTTTTTACTAAATAATG+GGG - chr4.3:82505656-82505675 None:intergenic 10.0%
!! ACCTTATAGTAATATATAAA+TGG - chr4.3:82506219-82506238 None:intergenic 15.0%
!! ATTATTTGTATGTACTTAAT+AGG - chr4.3:82506063-82506082 None:intergenic 15.0%
!! TAAATGAAATATGAATTAGT+TGG - chr4.3:82505709-82505728 None:intergenic 15.0%
!! TCCATTTATATATTACTATA+AGG + chr4.3:82506215-82506234 MS.gene037520:intron 15.0%
!!! AAATATAGAATCTTTTGTTT+TGG + chr4.3:82506401-82506420 MS.gene037520:intron 15.0%
!!! ATTTTTTTACTAAATAATGG+GGG - chr4.3:82505655-82505674 None:intergenic 15.0%
!!! TTATTTTACTATAGTAAAGT+TGG + chr4.3:82505682-82505701 MS.gene037520:intron 15.0%
!! ATACGAAAATTAATAAGTAG+TGG - chr4.3:82505604-82505623 None:intergenic 20.0%
!! CCTGTTAAACAAAAAAAAAT+GGG - chr4.3:82505954-82505973 None:intergenic 20.0%
!! TAATCAAGACTTAAAAGTTA+AGG - chr4.3:82505466-82505485 None:intergenic 20.0%
!!! AATAGGCTTCTAAAAAATAT+TGG - chr4.3:82505982-82506001 None:intergenic 20.0%
!!! CCATTTTTTTTTGTTTAACA+GGG + chr4.3:82505952-82505971 MS.gene037520:intron 20.0%
! ACATTTCAATAAATACCAAG+TGG - chr4.3:82506143-82506162 None:intergenic 25.0%
! AGAGAAAATTTATATGGAGT+CGG - chr4.3:82506357-82506376 None:intergenic 25.0%
! ATTATCTTCAAAAGCAATCT+TGG + chr4.3:82507026-82507045 MS.gene037520:CDS 25.0%
! CCCTGTTAAACAAAAAAAAA+TGG - chr4.3:82505955-82505974 None:intergenic 25.0%
! CCTATTTAACTAAAAAAGTC+AGG + chr4.3:82505996-82506015 MS.gene037520:intron 25.0%
! GAAAATGAATAAGACCAATT+GGG - chr4.3:82506850-82506869 None:intergenic 25.0%
! TGAAAATGAATAAGACCAAT+TGG - chr4.3:82506851-82506870 None:intergenic 25.0%
!! AACCATAGATAAAGTCTTTT+AGG + chr4.3:82506024-82506043 MS.gene037520:intron 25.0%
!!! ATTTTAGCAGTTTTTAGTCA+TGG - chr4.3:82506454-82506473 None:intergenic 25.0%
!!! CCCATTTTTTTTTGTTTAAC+AGG + chr4.3:82505951-82505970 MS.gene037520:intron 25.0%
!!! CCTGACTTTTTTAGTTAAAT+AGG - chr4.3:82505999-82506018 None:intergenic 25.0%
AAGACTTTATCTATGGTTTG+TGG - chr4.3:82506022-82506041 None:intergenic 30.0%
ATATACATACCACCATCTTT+GGG - chr4.3:82505440-82505459 None:intergenic 30.0%
CAAAAAGTCTAATATGTGCA+GGG - chr4.3:82505527-82505546 None:intergenic 30.0%
CGAAAATTAATAAGTAGTGG+AGG - chr4.3:82505601-82505620 None:intergenic 30.0%
GAAATCCTTATAGTACTTGA+AGG + chr4.3:82506734-82506753 MS.gene037520:CDS 30.0%
TATATACATACCACCATCTT+TGG - chr4.3:82505441-82505460 None:intergenic 30.0%
! AGCCAGAAATTTTAGATTGT+GGG - chr4.3:82506328-82506347 None:intergenic 30.0%
! ATTCATTTTCAACTGAACGT+GGG + chr4.3:82506860-82506879 MS.gene037520:CDS 30.0%
! TAATTTGCAGTGTTTGTGAA+CGG + chr4.3:82506503-82506522 MS.gene037520:intron 30.0%
! TATTCATTTTCAACTGAACG+TGG + chr4.3:82506859-82506878 MS.gene037520:CDS 30.0%
!!! GAATCTTTTGTTTTGGTTCA+CGG + chr4.3:82506408-82506427 MS.gene037520:intron 30.0%
AATTTGCAGTGTTTGTGAAC+GGG + chr4.3:82506504-82506523 MS.gene037520:intron 35.0%
ACGATTAGTGTTGTCTTGAT+TGG - chr4.3:82506786-82506805 None:intergenic 35.0%
ATACTTCCTTGTTAGCATCT+TGG + chr4.3:82505343-82505362 MS.gene037520:CDS 35.0%
CCAAAAAGTCTAATATGTGC+AGG - chr4.3:82505528-82505547 None:intergenic 35.0%
CTTATCCACTTGAAAAGCTT+TGG - chr4.3:82506996-82507015 None:intergenic 35.0%
GAAAATTTATATGGAGTCGG+TGG - chr4.3:82506354-82506373 None:intergenic 35.0%
GGCCTAAAAGACTTTATCTA+TGG - chr4.3:82506029-82506048 None:intergenic 35.0%
TATACATACCACCATCTTTG+GGG - chr4.3:82505439-82505458 None:intergenic 35.0%
TATTTGGACTCAACACACTT+GGG + chr4.3:82506642-82506661 MS.gene037520:CDS 35.0%
TCTTCAAACATGTTGAAGCT+GGG + chr4.3:82506567-82506586 MS.gene037520:CDS 35.0%
TTCTTCAAACATGTTGAAGC+TGG + chr4.3:82506566-82506585 MS.gene037520:CDS 35.0%
! CCAGAAATTTTAGATTGTGG+GGG - chr4.3:82506326-82506345 None:intergenic 35.0%
! CCTGCACATATTAGACTTTT+TGG + chr4.3:82505525-82505544 MS.gene037520:intron 35.0%
! GAGCCAGAAATTTTAGATTG+TGG - chr4.3:82506329-82506348 None:intergenic 35.0%
! GCCAGAAATTTTAGATTGTG+GGG - chr4.3:82506327-82506346 None:intergenic 35.0%
! TAAAGTCTTTTAGGCCAACA+AGG + chr4.3:82506033-82506052 MS.gene037520:intron 35.0%
!! TCTTACCAAAGCTTTTCAAG+TGG + chr4.3:82506988-82507007 MS.gene037520:CDS 35.0%
!!! TTTGGTTTTACTCCATACAG+TGG + chr4.3:82505543-82505562 MS.gene037520:intron 35.0%
AATTTATATGGAGTCGGTGG+CGG - chr4.3:82506351-82506370 None:intergenic 40.0%
ACTTAATAGGCCAACCTTGT+TGG - chr4.3:82506050-82506069 None:intergenic 40.0%
AGAACTTCGTCGGAAATAAG+AGG - chr4.3:82506971-82506990 None:intergenic 40.0%
ATAAGGATTTCCGTTCCTCT+AGG - chr4.3:82506725-82506744 None:intergenic 40.0%
CAACACAAAAGTCTTGGAGA+GGG - chr4.3:82505406-82505425 None:intergenic 40.0%
CAACACACTTGGGATATCTT+TGG + chr4.3:82506652-82506671 MS.gene037520:CDS 40.0%
CTATTTGGACTCAACACACT+TGG + chr4.3:82506641-82506660 MS.gene037520:CDS 40.0%
CTTGAAGGCAGTCTTTATGT+TGG + chr4.3:82506749-82506768 MS.gene037520:CDS 40.0%
GACTGCCTTCAAGTACTATA+AGG - chr4.3:82506742-82506761 None:intergenic 40.0%
GATGTGTTTGTGTTCCCAAT+TGG + chr4.3:82506833-82506852 MS.gene037520:CDS 40.0%
GTCACTGTAGACCAACTATT+TGG + chr4.3:82506626-82506645 MS.gene037520:CDS 40.0%
GTGTGTTGAGTCCAAATAGT+TGG - chr4.3:82506640-82506659 None:intergenic 40.0%
TAAGGATTTCCGTTCCTCTA+GGG - chr4.3:82506724-82506743 None:intergenic 40.0%
TGATGGCAACACAAAAGTCT+TGG - chr4.3:82505412-82505431 None:intergenic 40.0%
TTTCAACTGAACGTGGGATA+TGG + chr4.3:82506866-82506885 MS.gene037520:CDS 40.0%
TTTGCAACTGTGATAACTCC+TGG - chr4.3:82506929-82506948 None:intergenic 40.0%
! AAAATGGGCTCACCTAATGT+AGG - chr4.3:82505939-82505958 None:intergenic 40.0%
! AAATAATGGGGGCATGAAGT+TGG - chr4.3:82505644-82505663 None:intergenic 40.0%
! AGCTTTGGTAAGAACTTCGT+CGG - chr4.3:82506981-82507000 None:intergenic 40.0%
! ATCTTCAGCTTTAACTAGCG+CGG - chr4.3:82506546-82506565 None:intergenic 40.0%
! CCCCCACAATCTAAAATTTC+TGG + chr4.3:82506323-82506342 MS.gene037520:intron 40.0%
! TTCACCAAAGTGCTCAACAA+GGG + chr4.3:82506809-82506828 MS.gene037520:CDS 40.0%
!! GAAGTCACTGACTTGAGAAT+TGG - chr4.3:82506121-82506140 None:intergenic 40.0%
AAAAGTCTTGGAGAGGGCTA+GGG - chr4.3:82505400-82505419 None:intergenic 45.0%
AGCTACATGTACCCTTGTGT+GGG + chr4.3:82505569-82505588 MS.gene037520:intron 45.0%
ATCAAAGACCCCAAAGATGG+TGG + chr4.3:82505428-82505447 MS.gene037520:CDS 45.0%
ATCACCCTTGTTGAGCACTT+TGG - chr4.3:82506816-82506835 None:intergenic 45.0%
ATGTAGGCCAGGTTAAAGGT+CGG - chr4.3:82505923-82505942 None:intergenic 45.0%
CCAAAGCCAAGATGCTAACA+AGG - chr4.3:82505352-82505371 None:intergenic 45.0%
CCTAATGTAGGCCAGGTTAA+AGG - chr4.3:82505927-82505946 None:intergenic 45.0%
CCTTGTTAGCATCTTGGCTT+TGG + chr4.3:82505349-82505368 MS.gene037520:CDS 45.0%
CCTTTAACCTGGCCTACATT+AGG + chr4.3:82505924-82505943 MS.gene037520:intron 45.0%
GCAACACAAAAGTCTTGGAG+AGG - chr4.3:82505407-82505426 None:intergenic 45.0%
GGAATAGGCTGAGTCAAGTT+AGG - chr4.3:82506094-82506113 None:intergenic 45.0%
GGACTTAGCAGTCAAAATCC+AGG + chr4.3:82506908-82506927 MS.gene037520:CDS 45.0%
TCAAGTCAGTGACTTCCACT+TGG + chr4.3:82506125-82506144 MS.gene037520:intron 45.0%
TCACATTCACCCTAGAGGAA+CGG + chr4.3:82506712-82506731 MS.gene037520:CDS 45.0%
TTGAGAATTGGAGCGGGAAT+AGG - chr4.3:82506109-82506128 None:intergenic 45.0%
! CTTCACCAAAGTGCTCAACA+AGG + chr4.3:82506808-82506827 MS.gene037520:CDS 45.0%
! GGTTTTACTCCATACAGTGG+CGG + chr4.3:82505546-82505565 MS.gene037520:intron 45.0%
! GTCTTTTAGGCCAACAAGGT+TGG + chr4.3:82506037-82506056 MS.gene037520:intron 45.0%
! TCTTCAGCTTTAACTAGCGC+GGG - chr4.3:82506545-82506564 None:intergenic 45.0%
! TGAGGTGGGTTTAAACCCTT+AGG - chr4.3:82506695-82506714 None:intergenic 45.0%
!! ACCATCTTTGGGGTCTTTGA+TGG - chr4.3:82505429-82505448 None:intergenic 45.0%
!! ACTGACTTGAGAATTGGAGC+GGG - chr4.3:82506115-82506134 None:intergenic 45.0%
!! CACTGACTTGAGAATTGGAG+CGG - chr4.3:82506116-82506135 None:intergenic 45.0%
!!! CGCGTTGATTTTGAGCCTAA+GGG + chr4.3:82506677-82506696 MS.gene037520:CDS 45.0%
!!! TCGCGTTGATTTTGAGCCTA+AGG + chr4.3:82506676-82506695 MS.gene037520:CDS 45.0%
!!! TTTTGGTTCACGGTGTTTCC+TGG + chr4.3:82506418-82506437 MS.gene037520:intron 45.0%
!! AAAAAAAAAATACATATTAT+AGG - chr4.3:82506262-82506281 None:intergenic 5.0%
!!! TTATTTTTTTACTAAATAAT+GGG - chr4.3:82505657-82505676 None:intergenic 5.0%
!!! TTTATTTTTTTACTAAATAA+TGG - chr4.3:82505658-82505677 None:intergenic 5.0%
AGGGCTAGGGTCATAAGCAA+AGG - chr4.3:82505387-82505406 None:intergenic 50.0%
AGTCATGGCCTAGCTAAACC+AGG - chr4.3:82506439-82506458 None:intergenic 50.0%
CAAAAGTCTTGGAGAGGGCT+AGG - chr4.3:82505401-82505420 None:intergenic 50.0%
CGGTGTTTCCTGGTTTAGCT+AGG + chr4.3:82506428-82506447 MS.gene037520:intron 50.0%
GAGCTACATGTACCCTTGTG+TGG + chr4.3:82505568-82505587 MS.gene037520:intron 50.0%
GCCATCAAAGACCCCAAAGA+TGG + chr4.3:82505425-82505444 MS.gene037520:CDS 50.0%
TGTAGGCCAGGTTAAAGGTC+GGG - chr4.3:82505922-82505941 None:intergenic 50.0%
TGTCAACCCGACCTTTAACC+TGG + chr4.3:82505913-82505932 MS.gene037520:intron 50.0%
TTGAGAGCCTAGTGCGTTAG+AGG - chr4.3:82506597-82506616 None:intergenic 50.0%
! CTTCAGCTTTAACTAGCGCG+GGG - chr4.3:82506544-82506563 None:intergenic 50.0%
!! CTCTAGGGTGAATGTGAGGT+GGG - chr4.3:82506709-82506728 None:intergenic 50.0%
!! GTTCCTCTAGGGTGAATGTG+AGG - chr4.3:82506713-82506732 None:intergenic 50.0%
CATGTAGCTCCGCCACTGTA+TGG - chr4.3:82505558-82505577 None:intergenic 55.0%
CCACCTCACATTCACCCTAG+AGG + chr4.3:82506707-82506726 MS.gene037520:CDS 55.0%
GGGAATGCCTCTAACGCACT+AGG + chr4.3:82506587-82506606 MS.gene037520:CDS 55.0%
! GGCAACGCTGTTGCTATTGC+TGG + chr4.3:82506887-82506906 MS.gene037520:CDS 55.0%
!! CCTCTAGGGTGAATGTGAGG+TGG - chr4.3:82506710-82506729 None:intergenic 55.0%
GGGCTCACCTAATGTAGGCC+AGG - chr4.3:82505934-82505953 None:intergenic 60.0%
! GGAGGCACATGCCCACACAA+GGG - chr4.3:82505583-82505602 None:intergenic 60.0%
! TGGAGGCACATGCCCACACA+AGG - chr4.3:82505584-82505603 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 82505332 82507063 82505332 ID=MS.gene037520
chr4.3 mRNA 82505332 82507063 82505332 ID=MS.gene037520.t1;Parent=MS.gene037520
chr4.3 exon 82505332 82505449 82505332 ID=MS.gene037520.t1.exon1;Parent=MS.gene037520.t1
chr4.3 CDS 82505332 82505449 82505332 ID=cds.MS.gene037520.t1;Parent=MS.gene037520.t1
chr4.3 exon 82506513 82507063 82506513 ID=MS.gene037520.t1.exon2;Parent=MS.gene037520.t1
chr4.3 CDS 82506513 82507063 82506513 ID=cds.MS.gene037520.t1;Parent=MS.gene037520.t1
Gene Sequence

>MS.gene037520

ATGAAGATTATATACTTCCTTGTTAGCATCTTGGCTTTGGCATCATCTGTTGCCTTTGCTTATGACCCTAGCCCTCTCCAAGACTTTTGTGTTGCCATCAAAGACCCCAAAGATGGTGGTATGTATATATTCCTTAACTTTTAAGTCTTGATTAATTACTACTCAATTTTTAAGCTTAAATATGCAAATGTTCCCTGCACATATTAGACTTTTTGGTTTTACTCCATACAGTGGCGGAGCTACATGTACCCTTGTGTGGGCATGTGCCTCCACTACTTATTAATTTTCGTATATAACATTATTGTTAAGCCAACTTCATGCCCCCATTATTTAGTAAAAAAATAAAAAATTTATTTTACTATAGTAAAGTTGGTCCAACTAATTCATATTTCATTTATTGATAAATTGATTCATTAATCATAACATATATTTCTTTTGTGCTATAAGTGATTCATTTGTAATAAAAATTTGTTCCAAATTAAATCATCATTTTAATTTTCAATGCAATCATAGACACTTTTTTTTTATTAATATACACTAATAGTTGCATTATTTGCATTTTGCAAGTACATGTGACAACATGTCAACCCGACCTTTAACCTGGCCTACATTAGGTGAGCCCATTTTTTTTTGTTTAACAGGGAATACCAATATTTTTTAGAAGCCTATTTAACTAAAAAAGTCAGGCCACAAACCATAGATAAAGTCTTTTAGGCCAACAAGGTTGGCCTATTAAGTACATACAAATAATTTGCAAAACCTAACTTGACTCAGCCTATTCCCGCTCCAATTCTCAAGTCAGTGACTTCCACTTGGTATTTATTGAAATGTAAATAAATTAATATTTGATAAGAGTAATTTTGAAAAGTATTATTATTTCACTTCCATTTATATATTACTATAAGGTTATTATTTTACTTTCATCTTCCTATAATATGTATTTTTTTTTTTTGTAAAATAACATATTTAGTATTATAGTATATGGATATCGCCCCCACAATCTAAAATTTCTGGCTCCGCCACCGACTCCATATAAATTTTCTCTTTTTTTGTTTGTTTTAGTCTTTGTAAATATAGAATCTTTTGTTTTGGTTCACGGTGTTTCCTGGTTTAGCTAGGCCATGACTAAAAACTGCTAAAATTTCTTTGATATAACAAAACTATGATGTAATAATTTGCAGTGTTTGTGAACGGGAAATTCTGTAAAGACCCCGCGCTAGTTAAAGCTGAAGATTTCTTCAAACATGTTGAAGCTGGGAATGCCTCTAACGCACTAGGCTCTCAAGTAACTCAAGTCACTGTAGACCAACTATTTGGACTCAACACACTTGGGATATCTTTGGCTCGCGTTGATTTTGAGCCTAAGGGTTTAAACCCACCTCACATTCACCCTAGAGGAACGGAAATCCTTATAGTACTTGAAGGCAGTCTTTATGTTGGATTTGTCACATCCAATCAAGACAACACTAATCGTCTCTTCACCAAAGTGCTCAACAAGGGTGATGTGTTTGTGTTCCCAATTGGTCTTATTCATTTTCAACTGAACGTGGGATATGGCAACGCTGTTGCTATTGCTGGACTTAGCAGTCAAAATCCAGGAGTTATCACAGTTGCAAATGCTTTGTTTAAATCTGATCCTCTTATTTCCGACGAAGTTCTTACCAAAGCTTTTCAAGTGGATAAGAGCATAATTGATTATCTTCAAAAGCAATCTTGGTATGACAACAACTAG
Protein sequence

>MS.gene037520.t1

MKIIYFLVSILALASSVAFAYDPSPLQDFCVAIKDPKDGVFVNGKFCKDPALVKAEDFFKHVEAGNASNALGSQVTQVTVDQLFGLNTLGISLARVDFEPKGLNPPHIHPRGTEILIVLEGSLYVGFVTSNQDNTNRLFTKVLNKGDVFVFPIGLIHFQLNVGYGNAVAIAGLSSQNPGVITVANALFKSDPLISDEVLTKAFQVDKSIIDYLQKQSWYDNN