AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene037516

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037516.t1 MTR_5g046410 98.190 221 4 0 1 221 1 221 1.02e-160 443
MS.gene037516.t1 MTR_5g046430 98.190 221 4 0 1 221 1 221 1.02e-160 443
MS.gene037516.t1 MTR_4g017030 98.190 221 4 0 1 221 1 221 1.02e-160 443
MS.gene037516.t1 MTR_4g017050 97.738 221 5 0 1 221 1 221 3.47e-160 441
MS.gene037516.t1 MTR_2g019250 96.380 221 8 0 1 221 1 221 1.15e-158 437
MS.gene037516.t1 MTR_4g017040 96.833 221 7 0 1 221 1 221 1.74e-158 437
MS.gene037516.t1 MTR_1g079490 81.532 222 40 1 1 221 1 222 9.02e-131 367
MS.gene037516.t1 MTR_6g005360 75.113 221 54 1 1 221 1 220 2.25e-118 336
MS.gene037516.t1 MTR_6g005330 76.577 222 49 3 1 221 1 220 4.16e-118 335
MS.gene037516.t1 MTR_6g005340 77.064 218 48 2 4 221 5 220 4.30e-118 335
MS.gene037516.t1 MTR_6g005310 76.959 217 48 2 4 220 5 219 1.44e-117 334
MS.gene037516.t1 MTR_6g005350 63.014 219 78 3 3 221 4 219 2.57e-90 265
MS.gene037516.t1 MTR_6g005380 61.058 208 81 0 1 208 1 208 5.72e-88 258
MS.gene037516.t1 MTR_1g023770 59.447 217 77 3 1 215 1 208 4.02e-81 241
MS.gene037516.t1 MTR_2g030855 60.500 200 69 4 19 215 19 211 6.34e-78 233
MS.gene037516.t1 MTR_2g031270 59.701 201 70 5 19 215 19 212 1.15e-73 222
MS.gene037516.t1 MTR_2g030895 64.162 173 56 4 47 215 7 177 1.39e-71 216
MS.gene037516.t1 MTR_2g030865 63.584 173 57 4 47 215 7 177 1.23e-69 211
MS.gene037516.t1 MTR_1g085500 46.009 213 108 3 5 215 6 213 2.84e-60 188
MS.gene037516.t1 MTR_1g085140 46.000 200 101 3 18 215 20 214 1.23e-53 171
MS.gene037516.t1 MTR_7g111240 47.449 196 96 3 22 215 20 210 4.29e-52 167
MS.gene037516.t1 MTR_2g031300 54.386 171 53 4 47 214 7 155 1.22e-51 164
MS.gene037516.t1 MTR_7g111240 47.449 196 93 3 22 215 20 207 8.46e-51 164
MS.gene037516.t1 MTR_5g036570 45.685 197 99 4 22 215 27 218 2.29e-49 160
MS.gene037516.t1 MTR_1g112200 41.346 208 90 7 19 215 20 206 2.10e-43 145
MS.gene037516.t1 MTR_2g041550 41.981 212 106 5 4 213 7 203 1.32e-42 143
MS.gene037516.t1 MTR_1g060120 36.652 221 128 5 2 215 5 220 1.12e-41 141
MS.gene037516.t1 MTR_2g041570 38.498 213 114 5 4 214 7 204 7.41e-39 133
MS.gene037516.t1 MTR_2g041580 38.679 212 113 5 4 213 7 203 8.89e-39 133
MS.gene037516.t1 MTR_8g007300 38.532 218 118 5 4 219 11 214 3.71e-36 127
MS.gene037516.t1 MTR_8g020590 40.838 191 104 5 26 214 19 202 1.02e-34 123
MS.gene037516.t1 MTR_8g020750 39.594 197 99 5 1 190 1 184 2.53e-34 122
MS.gene037516.t1 MTR_3g019710 39.378 193 107 5 7 197 3 187 9.07e-33 118
MS.gene037516.t1 MTR_8g020630 38.674 181 98 5 1 179 1 170 1.63e-32 117
MS.gene037516.t1 MTR_2g036120 36.493 211 118 6 1 206 1 200 6.76e-31 113
MS.gene037516.t1 MTR_8g020650 35.938 192 112 5 26 215 22 204 3.12e-30 111
MS.gene037516.t1 MTR_8g020770 40.223 179 95 4 1 176 1 170 3.94e-30 111
MS.gene037516.t1 MTR_8g020670 33.641 217 129 6 1 215 1 204 1.97e-29 109
MS.gene037516.t1 MTR_3g019650 39.655 174 95 5 26 197 21 186 6.36e-29 108
MS.gene037516.t1 MTR_0284s0010 36.493 211 118 6 1 206 1 200 8.88e-29 107
MS.gene037516.t1 MTR_3g019680 36.816 201 108 7 1 197 1 186 1.01e-28 107
MS.gene037516.t1 MTR_2g044040 36.313 179 101 5 1 177 1 168 2.55e-28 106
MS.gene037516.t1 MTR_2g086640 33.641 217 118 7 2 214 7 201 1.11e-27 104
MS.gene037516.t1 MTR_2g045100 36.019 211 119 6 1 206 1 200 1.33e-27 104
MS.gene037516.t1 MTR_4g052770 34.703 219 117 6 1 214 6 203 1.78e-27 104
MS.gene037516.t1 MTR_4g052780 35.784 204 105 6 16 214 21 203 1.95e-27 103
MS.gene037516.t1 MTR_2g086620 41.985 131 70 3 85 214 77 202 2.89e-27 103
MS.gene037516.t1 MTR_3g096330 38.418 177 103 3 5 179 560 732 3.06e-27 109
MS.gene037516.t1 MTR_2g086630 41.221 131 71 3 85 214 77 202 7.60e-27 102
MS.gene037516.t1 MTR_5g031460 33.810 210 125 5 1 206 1 200 1.26e-26 102
MS.gene037516.t1 MTR_8g020610 33.953 215 125 7 1 213 1 200 1.06e-25 99.8
MS.gene037516.t1 MTR_8g020760 33.180 217 132 6 1 215 1 206 4.18e-25 98.2
MS.gene037516.t1 MTR_7g011340 37.097 186 105 5 26 206 22 200 1.48e-24 96.7
MS.gene037516.t1 MTR_4g010340 36.782 174 102 4 43 215 34 200 1.49e-24 96.7
MS.gene037516.t1 MTR_8g020763 36.095 169 99 3 43 206 17 181 8.45e-24 94.4
MS.gene037516.t1 MTR_2g019780 32.420 219 132 7 1 215 1 207 9.96e-24 94.7
MS.gene037516.t1 MTR_3g073170 35.484 186 104 6 27 206 26 201 2.98e-23 93.2
MS.gene037516.t1 MTR_3g019810 33.171 205 115 9 1 202 107 292 1.00e-18 83.2
MS.gene037516.t1 MTR_2g030915 48.684 76 24 1 93 168 1 61 1.19e-17 75.5
MS.gene037516.t1 MTR_4g027415 37.500 128 71 4 26 151 22 142 1.24e-15 71.2
MS.gene037516.t1 MTR_2g072560 36.066 122 69 4 1 120 1 115 6.03e-13 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037516.t1 AT3G05950 65.471 223 69 4 6 221 7 228 8.67e-96 279
MS.gene037516.t1 AT5G39150 64.220 218 75 2 1 215 1 218 7.83e-95 276
MS.gene037516.t1 AT5G39180 64.220 218 75 2 1 215 1 218 1.60e-94 275
MS.gene037516.t1 AT5G39120 64.220 218 75 2 1 215 1 218 3.28e-94 275
MS.gene037516.t1 AT5G39160 64.840 219 72 3 1 215 1 218 1.23e-92 271
MS.gene037516.t1 AT5G39110 64.455 211 73 1 7 215 9 219 1.80e-92 270
MS.gene037516.t1 AT5G39190 64.840 219 72 3 1 215 1 218 1.90e-92 270
MS.gene037516.t1 AT5G39130 63.470 219 75 3 1 215 1 218 6.92e-92 269
MS.gene037516.t1 AT5G39160 62.557 219 73 3 1 215 1 214 6.44e-88 259
MS.gene037516.t1 AT4G14630 60.731 219 82 2 1 215 3 221 1.69e-87 258
MS.gene037516.t1 AT3G04200 60.465 215 82 3 1 213 6 219 4.43e-84 249
MS.gene037516.t1 AT5G39190 58.986 217 66 2 1 215 1 196 1.92e-80 239
MS.gene037516.t1 AT5G39160 58.525 217 67 2 1 215 1 196 6.10e-80 238
MS.gene037516.t1 AT5G38910 59.545 220 82 4 1 215 1 218 1.35e-78 235
MS.gene037516.t1 AT5G38960 60.697 201 75 3 18 215 22 221 8.72e-78 233
MS.gene037516.t1 AT5G38940 60.714 196 74 2 22 215 26 220 1.38e-77 233
MS.gene037516.t1 AT5G38940 60.714 196 74 2 22 215 50 244 2.19e-77 233
MS.gene037516.t1 AT5G38930 61.616 198 73 2 20 215 24 220 2.38e-77 232
MS.gene037516.t1 AT3G04170 55.981 209 86 3 8 214 12 216 7.33e-75 226
MS.gene037516.t1 AT3G04190 56.219 201 85 2 15 213 19 218 7.11e-74 223
MS.gene037516.t1 AT3G04150 54.378 217 93 3 3 214 4 219 7.23e-73 221
MS.gene037516.t1 AT3G04150 51.528 229 94 3 3 214 4 232 4.70e-71 217
MS.gene037516.t1 AT3G04180 54.726 201 88 2 15 213 19 218 8.53e-71 215
MS.gene037516.t1 AT1G02335 46.465 198 99 3 20 215 22 214 7.02e-56 177
MS.gene037516.t1 AT5G38910 48.636 220 69 4 1 215 1 181 4.17e-55 174
MS.gene037516.t1 AT3G62020 46.009 213 106 5 6 215 8 214 6.42e-55 175
MS.gene037516.t1 AT1G09560 45.274 201 103 3 17 215 19 214 2.45e-53 171
MS.gene037516.t1 AT3G05930 46.798 203 99 4 3 202 5 201 2.19e-52 168
MS.gene037516.t1 AT1G18970 46.000 200 99 4 18 215 25 217 3.67e-51 165
MS.gene037516.t1 AT5G26700 46.875 192 88 5 26 213 25 206 1.36e-50 164
MS.gene037516.t1 AT1G18980 45.500 200 100 4 18 215 25 217 6.11e-49 159
MS.gene037516.t1 AT3G62020 46.667 180 90 3 39 215 9 185 2.02e-47 155
MS.gene037516.t1 AT3G10080 40.099 202 112 5 18 215 22 218 3.72e-44 147
MS.gene037516.t1 AT1G10460 41.414 198 108 5 22 214 19 213 3.76e-44 147
MS.gene037516.t1 AT3G10080 40.099 202 112 5 18 215 93 289 1.81e-43 148
MS.gene037516.t1 AT5G20630 41.905 210 110 6 1 208 1 200 4.15e-42 142
MS.gene037516.t1 AT5G61750 41.530 183 102 2 6 186 3 182 7.01e-40 136
MS.gene037516.t1 AT1G72610 41.053 190 103 6 26 213 19 201 3.96e-34 121
MS.gene037516.t1 AT1G74820 38.660 194 110 4 24 215 38 224 4.43e-33 119
MS.gene037516.t1 AT5G38950 54.167 72 33 0 145 216 2 73 1.39e-18 78.2
MS.gene037516.t1 AT5G39100 72.000 50 14 0 7 56 9 58 7.86e-18 74.7

Find 58 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATATACATACCACCATCTT+TGG 0.255130 4.3:+82552296 None:intergenic
TTTCAACTGAATGTGGGATA+TGG 0.310660 4.3:-82551687 MS.gene037516:CDS
CCTTGTTAGCATCTTGGCTT+TGG 0.311083 4.3:-82552385 MS.gene037516:CDS
TTGAGATCCTAGTGCATTAG+AGG 0.333822 4.3:+82551956 None:intergenic
CTTATCCACTTGAAAAGCTT+TGG 0.335788 4.3:+82551560 None:intergenic
TAACACGCTTGGTATATCTT+TGG 0.340502 4.3:-82551898 MS.gene037516:CDS
TGAAAATGAATAAGACCAAT+TGG 0.356438 4.3:+82551705 None:intergenic
ATACTTCCTTGTTAGCATCT+TGG 0.361887 4.3:-82552391 MS.gene037516:CDS
GGCAACGCCGTTGCTATTGC+TGG 0.394673 4.3:-82551666 MS.gene037516:CDS
GAGTGCCTTCGAGTACTATA+AGG 0.397511 4.3:+82551811 None:intergenic
TTCTTCAAACATGTTGAAGC+TGG 0.402098 4.3:-82551984 MS.gene037516:CDS
ATATACATACCACCATCTTT+GGG 0.405165 4.3:+82552297 None:intergenic
CTCTAGGGTGGATGTGAGGT+GGG 0.411548 4.3:+82551844 None:intergenic
GATGTGTTTGTGTTCCCAAT+TGG 0.424230 4.3:-82551720 MS.gene037516:CDS
TAAAGAGCCGGTTGTTGTCT+TGG 0.425026 4.3:+82551763 None:intergenic
CAAAAGTCTTGGAGAGGGCT+AGG 0.434671 4.3:+82552336 None:intergenic
TAATTTGCAGTATTCGTGAA+TGG 0.437869 4.3:-82552047 MS.gene037516:intron
ATTATCTTCAAAAGCAGTCT+TGG 0.442184 4.3:-82551527 MS.gene037516:CDS
CTCGAAGGCACTCTTTATGT+TGG 0.443268 4.3:-82551801 MS.gene037516:CDS
TCTTCAGCTTTAACAAGTGC+GGG 0.444792 4.3:+82552008 None:intergenic
GTCACTGTAGACCAACTATT+CGG 0.448530 4.3:-82551924 MS.gene037516:CDS
CATCAAACCAAGACAACAAC+CGG 0.452231 4.3:-82551770 MS.gene037516:CDS
TTTGCAACTGTGATAACTCC+TGG 0.466515 4.3:+82551627 None:intergenic
ATAAGGATCTCTGTTCCTCT+AGG 0.468428 4.3:+82551828 None:intergenic
TGATTGCAACACAAAAGTCT+TGG 0.490396 4.3:+82552325 None:intergenic
AATTTGCAGTATTCGTGAAT+GGG 0.491761 4.3:-82552046 MS.gene037516:intron
CCTCTAGGGTGGATGTGAGG+TGG 0.496940 4.3:+82551843 None:intergenic
TCTTCAAACATGTTGAAGCT+GGG 0.501315 4.3:-82551983 MS.gene037516:CDS
CTATTCGGACTTAACACGCT+TGG 0.510323 4.3:-82551909 MS.gene037516:CDS
GAAAATGAATAAGACCAATT+GGG 0.510497 4.3:+82551706 None:intergenic
AGAACTTCGTCGGAAATAAG+AGG 0.511108 4.3:+82551585 None:intergenic
GCGTGTTAAGTCCGAATAGT+TGG 0.534502 4.3:+82551913 None:intergenic
TGAGGTGGGTTTAAACCCTT+AGG 0.537084 4.3:+82551858 None:intergenic
AATAGTTGGTCTACAGTGAC+AGG 0.537258 4.3:+82551927 None:intergenic
ATCTTCAGCTTTAACAAGTG+CGG 0.543612 4.3:+82552007 None:intergenic
CTTTACCAAGGTGCTCAACA+AGG 0.544491 4.3:-82551745 MS.gene037516:CDS
GCAATCAAAGACCCCAAAGA+TGG 0.554146 4.3:-82552309 MS.gene037516:CDS
AGCTTTGGTAAGAACTTCGT+CGG 0.555884 4.3:+82551575 None:intergenic
CCAAAGCCAAGATGCTAACA+AGG 0.556137 4.3:+82552385 None:intergenic
AAAAGTCTTGGAGAGGGCTA+GGG 0.564154 4.3:+82552337 None:intergenic
ATCACCCTTGTTGAGCACCT+TGG 0.567836 4.3:+82551740 None:intergenic
TAAGGATCTCTGTTCCTCTA+GGG 0.571738 4.3:+82551829 None:intergenic
GGACTTAGCAGTCAAAATCC+AGG 0.578792 4.3:-82551645 MS.gene037516:CDS
TGAGCACCTTGGTAAAGAGC+CGG 0.579420 4.3:+82551751 None:intergenic
GGGAATGCCTCTAATGCACT+AGG 0.589101 4.3:-82551963 MS.gene037516:CDS
GGATCTCTGTTCCTCTAGGG+TGG 0.590427 4.3:+82551832 None:intergenic
AGGGCTAGGGTCATAAGCAA+AGG 0.622370 4.3:+82552350 None:intergenic
ATCAAAGACCCCAAAGATGG+TGG 0.628410 4.3:-82552306 MS.gene037516:intron
TATACATACCACCATCTTTG+GGG 0.639620 4.3:+82552298 None:intergenic
CAACACAAAAGTCTTGGAGA+GGG 0.641422 4.3:+82552331 None:intergenic
GTTCCTCTAGGGTGGATGTG+AGG 0.650118 4.3:+82551840 None:intergenic
GCAACACAAAAGTCTTGGAG+AGG 0.651085 4.3:+82552330 None:intergenic
CCACCTCACATCCACCCTAG+AGG 0.660499 4.3:-82551843 MS.gene037516:CDS
CTTCAGCTTTAACAAGTGCG+GGG 0.662073 4.3:+82552009 None:intergenic
GAGATCCTTATAGTACTCGA+AGG 0.666965 4.3:-82551816 MS.gene037516:CDS
TTTACCAAGGTGCTCAACAA+GGG 0.669433 4.3:-82551744 MS.gene037516:CDS
CAACAACCGGCTCTTTACCA+AGG 0.674974 4.3:-82551757 MS.gene037516:CDS
GCTAAGTCCAGCAATAGCAA+CGG 0.679928 4.3:+82551659 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTCAATAAATATATTTTTA+TGG - chr4.3:82551723-82551742 MS.gene037516:CDS 10.0%
!! TAAAATGTTTGATATTTGTA+AGG + chr4.3:82551696-82551715 None:intergenic 15.0%
!!! TTAATTACTACTCAATTTTT+AGG - chr4.3:82551652-82551671 MS.gene037516:CDS 15.0%
!!! ATGTATAGAATCTTTTGTTT+TGG - chr4.3:82551759-82551778 MS.gene037516:CDS 20.0%
! GAAAATGAATAAGACCAATT+GGG + chr4.3:82552211-82552230 None:intergenic 25.0%
! TGAAAATGAATAAGACCAAT+TGG + chr4.3:82552212-82552231 None:intergenic 25.0%
! TGAGTAGTAATTAAGACTTA+AGG + chr4.3:82551646-82551665 None:intergenic 25.0%
!! ATTCATTTTCAACTGAATGT+GGG - chr4.3:82552221-82552240 MS.gene037516:intron 25.0%
!! TATTCATTTTCAACTGAATG+TGG - chr4.3:82552220-82552239 MS.gene037516:intron 25.0%
AATTTGCAGTATTCGTGAAT+GGG - chr4.3:82551868-82551887 MS.gene037516:CDS 30.0%
ATATACATACCACCATCTTT+GGG + chr4.3:82551620-82551639 None:intergenic 30.0%
ATTATCTTCAAAAGCAGTCT+TGG - chr4.3:82552387-82552406 MS.gene037516:CDS 30.0%
TATATACATACCACCATCTT+TGG + chr4.3:82551621-82551640 None:intergenic 30.0%
! TAATTTGCAGTATTCGTGAA+TGG - chr4.3:82551867-82551886 MS.gene037516:CDS 30.0%
ATACTTCCTTGTTAGCATCT+TGG - chr4.3:82551523-82551542 MS.gene037516:CDS 35.0%
CTTATCCACTTGAAAAGCTT+TGG + chr4.3:82552357-82552376 None:intergenic 35.0%
TAACACGCTTGGTATATCTT+TGG - chr4.3:82552016-82552035 MS.gene037516:CDS 35.0%
TATACATACCACCATCTTTG+GGG + chr4.3:82551619-82551638 None:intergenic 35.0%
TCTTCAAACATGTTGAAGCT+GGG - chr4.3:82551931-82551950 MS.gene037516:CDS 35.0%
TGATTGCAACACAAAAGTCT+TGG + chr4.3:82551592-82551611 None:intergenic 35.0%
TTCTTCAAACATGTTGAAGC+TGG - chr4.3:82551930-82551949 MS.gene037516:CDS 35.0%
TTTCAACTGAATGTGGGATA+TGG - chr4.3:82552227-82552246 MS.gene037516:intron 35.0%
! ATCTTCAGCTTTAACAAGTG+CGG + chr4.3:82551910-82551929 None:intergenic 35.0%
!! TCTTACCAAAGCTTTTCAAG+TGG - chr4.3:82552349-82552368 MS.gene037516:CDS 35.0%
AGAACTTCGTCGGAAATAAG+AGG + chr4.3:82552332-82552351 None:intergenic 40.0%
ATAAGGATCTCTGTTCCTCT+AGG + chr4.3:82552089-82552108 None:intergenic 40.0%
CAACACAAAAGTCTTGGAGA+GGG + chr4.3:82551586-82551605 None:intergenic 40.0%
CATCAAACCAAGACAACAAC+CGG - chr4.3:82552144-82552163 MS.gene037516:intron 40.0%
GAGATCCTTATAGTACTCGA+AGG - chr4.3:82552098-82552117 MS.gene037516:intron 40.0%
GATGTGTTTGTGTTCCCAAT+TGG - chr4.3:82552194-82552213 MS.gene037516:intron 40.0%
GTCACTGTAGACCAACTATT+CGG - chr4.3:82551990-82552009 MS.gene037516:CDS 40.0%
TAAGGATCTCTGTTCCTCTA+GGG + chr4.3:82552088-82552107 None:intergenic 40.0%
TTGAGATCCTAGTGCATTAG+AGG + chr4.3:82551961-82551980 None:intergenic 40.0%
TTTGCAACTGTGATAACTCC+TGG + chr4.3:82552290-82552309 None:intergenic 40.0%
! AATAGTTGGTCTACAGTGAC+AGG + chr4.3:82551990-82552009 None:intergenic 40.0%
! AGCTTTGGTAAGAACTTCGT+CGG + chr4.3:82552342-82552361 None:intergenic 40.0%
! TCTTCAGCTTTAACAAGTGC+GGG + chr4.3:82551909-82551928 None:intergenic 40.0%
! TTTACCAAGGTGCTCAACAA+GGG - chr4.3:82552170-82552189 MS.gene037516:intron 40.0%
AAAAGTCTTGGAGAGGGCTA+GGG + chr4.3:82551580-82551599 None:intergenic 45.0%
AGCTAAACCAGCAATCACTG+AGG + chr4.3:82551786-82551805 None:intergenic 45.0%
ATCAAAGACCCCAAAGATGG+TGG - chr4.3:82551608-82551627 MS.gene037516:CDS 45.0%
CCAAAGCCAAGATGCTAACA+AGG + chr4.3:82551532-82551551 None:intergenic 45.0%
CCTTGTTAGCATCTTGGCTT+TGG - chr4.3:82551529-82551548 MS.gene037516:CDS 45.0%
CTATTCGGACTTAACACGCT+TGG - chr4.3:82552005-82552024 MS.gene037516:CDS 45.0%
GAGTGCCTTCGAGTACTATA+AGG + chr4.3:82552106-82552125 None:intergenic 45.0%
GCAACACAAAAGTCTTGGAG+AGG + chr4.3:82551587-82551606 None:intergenic 45.0%
GCAATCAAAGACCCCAAAGA+TGG - chr4.3:82551605-82551624 MS.gene037516:CDS 45.0%
GCGTGTTAAGTCCGAATAGT+TGG + chr4.3:82552004-82552023 None:intergenic 45.0%
GCTAAGTCCAGCAATAGCAA+CGG + chr4.3:82552258-82552277 None:intergenic 45.0%
GGACTTAGCAGTCAAAATCC+AGG - chr4.3:82552269-82552288 MS.gene037516:intron 45.0%
TAAAGAGCCGGTTGTTGTCT+TGG + chr4.3:82552154-82552173 None:intergenic 45.0%
! CTTCAGCTTTAACAAGTGCG+GGG + chr4.3:82551908-82551927 None:intergenic 45.0%
! CTTTACCAAGGTGCTCAACA+AGG - chr4.3:82552169-82552188 MS.gene037516:intron 45.0%
! TGAGGTGGGTTTAAACCCTT+AGG + chr4.3:82552059-82552078 None:intergenic 45.0%
! TTTTGGTCCTCAGTGATTGC+TGG - chr4.3:82551776-82551795 MS.gene037516:CDS 45.0%
!! CAGTGATTGCTGGTTTAGCT+AGG - chr4.3:82551786-82551805 MS.gene037516:CDS 45.0%
!! CTCGAAGGCACTCTTTATGT+TGG - chr4.3:82552113-82552132 MS.gene037516:intron 45.0%
AGGGCTAGGGTCATAAGCAA+AGG + chr4.3:82551567-82551586 None:intergenic 50.0%
ATCACCCTTGTTGAGCACCT+TGG + chr4.3:82552177-82552196 None:intergenic 50.0%
CAAAAGTCTTGGAGAGGGCT+AGG + chr4.3:82551581-82551600 None:intergenic 50.0%
GGGAATGCCTCTAATGCACT+AGG - chr4.3:82551951-82551970 MS.gene037516:CDS 50.0%
TGAGCACCTTGGTAAAGAGC+CGG + chr4.3:82552166-82552185 None:intergenic 50.0%
! CAACAACCGGCTCTTTACCA+AGG - chr4.3:82552157-82552176 MS.gene037516:intron 50.0%
!!! CGCGTTGATTTTGCGCCTAA+GGG - chr4.3:82552041-82552060 MS.gene037516:intron 50.0%
!!! TCGCGTTGATTTTGCGCCTA+AGG - chr4.3:82552040-82552059 MS.gene037516:CDS 50.0%
! GGATCTCTGTTCCTCTAGGG+TGG + chr4.3:82552085-82552104 None:intergenic 55.0%
!! CTCTAGGGTGGATGTGAGGT+GGG + chr4.3:82552073-82552092 None:intergenic 55.0%
!! GTTCCTCTAGGGTGGATGTG+AGG + chr4.3:82552077-82552096 None:intergenic 55.0%
CCACCTCACATCCACCCTAG+AGG - chr4.3:82552071-82552090 MS.gene037516:intron 60.0%
! GGCAACGCCGTTGCTATTGC+TGG - chr4.3:82552248-82552267 MS.gene037516:intron 60.0%
!! CCTCTAGGGTGGATGTGAGG+TGG + chr4.3:82552074-82552093 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 82551512 82552424 82551512 ID=MS.gene037516
chr4.3 mRNA 82551512 82552424 82551512 ID=MS.gene037516.t1;Parent=MS.gene037516
chr4.3 exon 82552307 82552424 82552307 ID=MS.gene037516.t1.exon1;Parent=MS.gene037516.t1
chr4.3 CDS 82552307 82552424 82552307 ID=cds.MS.gene037516.t1;Parent=MS.gene037516.t1
chr4.3 exon 82551512 82552059 82551512 ID=MS.gene037516.t1.exon2;Parent=MS.gene037516.t1
chr4.3 CDS 82551512 82552059 82551512 ID=cds.MS.gene037516.t1;Parent=MS.gene037516.t1
Gene Sequence

>MS.gene037516

ATGAAGGTTGTATACTTCCTTGTTAGCATCTTGGCTTTGGCATCATCTGTTGCCTTTGCTTATGACCCTAGCCCTCTCCAAGACTTTTGTGTTGCAATCAAAGACCCCAAAGATGGTGGTATGTATATATTCCTTAAGTCTTAATTACTACTCAATTTTTAGGCTTAAATATACAAATGCTCCTTACAAATATCAAACATTTTAGTTTTACTCTCAATAAATATATTTTTATGGTTTTAGTTCTTGTATGTATAGAATCTTTTGTTTTGGTCCTCAGTGATTGCTGGTTTAGCTAGGTCATGACTAGAAGAATTATACTAATGAATTTTTTTTATATAACAAAACTATGATTTGATAATTTGCAGTATTCGTGAATGGGAAATTCTGTAAAGACCCCGCACTTGTTAAAGCTGAAGATTTCTTCAAACATGTTGAAGCTGGGAATGCCTCTAATGCACTAGGATCTCAAGTGACTCCTGTCACTGTAGACCAACTATTCGGACTTAACACGCTTGGTATATCTTTGGCTCGCGTTGATTTTGCGCCTAAGGGTTTAAACCCACCTCACATCCACCCTAGAGGAACAGAGATCCTTATAGTACTCGAAGGCACTCTTTATGTTGGATTTGTCTCATCAAACCAAGACAACAACCGGCTCTTTACCAAGGTGCTCAACAAGGGTGATGTGTTTGTGTTCCCAATTGGTCTTATTCATTTTCAACTGAATGTGGGATATGGCAACGCCGTTGCTATTGCTGGACTTAGCAGTCAAAATCCAGGAGTTATCACAGTTGCAAATGCTTTGTTTAAATCTGATCCTCTTATTTCCGACGAAGTTCTTACCAAAGCTTTTCAAGTGGATAAGAGCATAATTGATTATCTTCAAAAGCAGTCTTGGTATGACAACAACTAG
Protein sequence

>MS.gene037516.t1

MKVVYFLVSILALASSVAFAYDPSPLQDFCVAIKDPKDGVFVNGKFCKDPALVKAEDFFKHVEAGNASNALGSQVTPVTVDQLFGLNTLGISLARVDFAPKGLNPPHIHPRGTEILIVLEGTLYVGFVSSNQDNNRLFTKVLNKGDVFVFPIGLIHFQLNVGYGNAVAIAGLSSQNPGVITVANALFKSDPLISDEVLTKAFQVDKSIIDYLQKQSWYDNN