AlfalfaGEDB | MS.gene50787

M. sativa cultivar XinJiangDaYe / MS.gene50787

KEGG: -
GO: GO:0003674 GO:0005488 GO:0030145 GO:0043167 GO:0043169 GO:0045735 GO:0046872 GO:0046914
PFAM: PF00190.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50787.t1	XP_003604729.1	98.2	221	4	0	1	221	1	221	1.20E-119	439.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50787.t1	Q9SFF9	65.5	223	69	4	6	221	7	228	8.6e-74	278.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50787.t1	G7JQ50	98.2	221	4	0	1	221	1	221	8.7e-120	439.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049661	MS.gene50787	-0.805846	1.10E-49	-1.69E-46
MS.gene049662	MS.gene50787	-0.804275	2.34E-49	-1.69E-46
MS.gene050464	MS.gene50787	-0.817919	2.57E-52	-1.69E-46
MS.gene050529	MS.gene50787	-0.819516	1.11E-52	-1.69E-46
MS.gene050670	MS.gene50787	-0.806811	6.86E-50	-1.69E-46
MS.gene050971	MS.gene50787	-0.810852	9.38E-51	-1.69E-46
MS.gene051158	MS.gene50787	0.837011	6.69E-57	-1.69E-46
MS.gene052188	MS.gene50787	-0.803484	3.41E-49	-1.69E-46
MS.gene052310	MS.gene50787	-0.803216	3.88E-49	-1.69E-46
MS.gene05233	MS.gene50787	0.811324	7.41E-51	-1.69E-46
MS.gene05240	MS.gene50787	0.805253	1.46E-49	-1.69E-46
MS.gene053031	MS.gene50787	0.807777	4.28E-50	-1.69E-46
MS.gene053406	MS.gene50787	0.82777	1.30E-54	-1.69E-46
MS.gene054290	MS.gene50787	0.830327	3.13E-55	-1.69E-46
MS.gene054349	MS.gene50787	-0.802929	4.44E-49	-1.69E-46
MS.gene054742	MS.gene50787	0.813606	2.35E-51	-1.69E-46
MS.gene055601	MS.gene50787	0.866081	3.70E-65	-1.69E-46
MS.gene055959	MS.gene50787	0.810487	1.12E-50	-1.69E-46
MS.gene056330	MS.gene50787	0.817922	2.56E-52	-1.69E-46
MS.gene056691	MS.gene50787	0.801194	1.01E-48	-1.69E-46
MS.gene056899	MS.gene50787	0.864747	9.73E-65	-1.69E-46
MS.gene057749	MS.gene50787	-0.803552	3.30E-49	-1.69E-46
MS.gene057823	MS.gene50787	-0.803536	3.33E-49	-1.69E-46
MS.gene058177	MS.gene50787	0.800029	1.75E-48	-1.69E-46
MS.gene058218	MS.gene50787	0.875552	2.82E-68	-1.69E-46
MS.gene058660	MS.gene50787	-0.808101	3.65E-50	-1.69E-46
MS.gene058928	MS.gene50787	-0.825719	4.02E-54	-1.69E-46
MS.gene059043	MS.gene50787	0.845882	3.08E-59	-1.69E-46
MS.gene05907	MS.gene50787	-0.809148	2.18E-50	-1.69E-46
MS.gene059294	MS.gene50787	-0.852016	6.09E-61	-1.69E-46
MS.gene059402	MS.gene50787	0.823565	1.29E-53	-1.69E-46
MS.gene061049	MS.gene50787	-0.808592	2.87E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene50787	MS.gene032596	PPI
MS.gene89775	MS.gene50787	PPI
MS.gene50787	MS.gene037519	PPI
MS.gene50787	MS.gene047464	PPI
MS.gene50787	MS.gene005041	PPI
MS.gene50787	MS.gene82860	PPI
MS.gene50787	MS.gene82859	PPI
MS.gene50786	MS.gene50787	PPI
MS.gene032601	MS.gene50787	PPI
MS.gene99166	MS.gene50787	PPI
MS.gene96244	MS.gene50787	PPI
MS.gene81739	MS.gene50787	PPI
MS.gene056870	MS.gene50787	PPI
MS.gene50787	MS.gene70062	PPI
MS.gene50787	MS.gene96244	PPI
MS.gene034877	MS.gene50787	PPI
MS.gene82858	MS.gene50787	PPI
MS.gene50787	MS.gene053031	PPI
MS.gene004411	MS.gene50787	PPI
MS.gene002728	MS.gene50787	PPI
MS.gene037517	MS.gene50787	PPI
MS.gene032596	MS.gene50787	PPI
MS.gene50787	MS.gene07591	PPI
MS.gene022634	MS.gene50787	PPI
MS.gene89772	MS.gene50787	PPI
MS.gene50787	MS.gene037520	PPI
MS.gene037516	MS.gene50787	PPI
MS.gene50790	MS.gene50787	PPI
MS.gene032598	MS.gene50787	PPI
MS.gene89773	MS.gene50787	PPI
MS.gene70063	MS.gene50787	PPI
MS.gene053033	MS.gene50787	PPI
MS.gene50787	MS.gene50789	PPI
MS.gene50787	MS.gene70063	PPI
MS.gene50787	MS.gene037518	PPI
MS.gene053027	MS.gene50787	PPI
MS.gene50787	MS.gene50788	PPI
MS.gene70064	MS.gene50787	PPI
MS.gene74887	MS.gene50787	PPI
MS.gene70062	MS.gene50787	PPI
MS.gene50787	MS.gene055423	PPI
MS.gene032599	MS.gene50787	PPI
MS.gene50787	MS.gene89774	PPI
MS.gene89774	MS.gene50787	PPI
MS.gene50787	MS.gene037851	PPI
MS.gene50787	MS.gene032597	PPI
MS.gene50787	MS.gene82855	PPI
MS.gene50787	MS.gene70061	PPI
MS.gene053029	MS.gene50787	PPI
MS.gene053030	MS.gene50787	PPI
MS.gene50787	MS.gene82857	PPI
MS.gene053026	MS.gene50787	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50787.t1	MTR_5g046410	98.190	221	4	0	1	221	1	221	1.02e-160	443
MS.gene50787.t1	MTR_5g046430	98.190	221	4	0	1	221	1	221	1.02e-160	443
MS.gene50787.t1	MTR_4g017030	98.190	221	4	0	1	221	1	221	1.02e-160	443
MS.gene50787.t1	MTR_4g017050	97.738	221	5	0	1	221	1	221	3.47e-160	441
MS.gene50787.t1	MTR_2g019250	96.380	221	8	0	1	221	1	221	1.15e-158	437
MS.gene50787.t1	MTR_4g017040	96.833	221	7	0	1	221	1	221	1.74e-158	437
MS.gene50787.t1	MTR_1g079490	81.532	222	40	1	1	221	1	222	9.02e-131	367
MS.gene50787.t1	MTR_6g005360	75.113	221	54	1	1	221	1	220	2.25e-118	336
MS.gene50787.t1	MTR_6g005330	76.577	222	49	3	1	221	1	220	4.16e-118	335
MS.gene50787.t1	MTR_6g005340	77.064	218	48	2	4	221	5	220	4.30e-118	335
MS.gene50787.t1	MTR_6g005310	76.959	217	48	2	4	220	5	219	1.44e-117	334
MS.gene50787.t1	MTR_6g005350	63.014	219	78	3	3	221	4	219	2.57e-90	265
MS.gene50787.t1	MTR_6g005380	61.058	208	81	0	1	208	1	208	5.72e-88	258
MS.gene50787.t1	MTR_1g023770	59.447	217	77	3	1	215	1	208	4.02e-81	241
MS.gene50787.t1	MTR_2g030855	60.500	200	69	4	19	215	19	211	6.34e-78	233
MS.gene50787.t1	MTR_2g031270	59.701	201	70	5	19	215	19	212	1.15e-73	222
MS.gene50787.t1	MTR_2g030895	64.162	173	56	4	47	215	7	177	1.39e-71	216
MS.gene50787.t1	MTR_2g030865	63.584	173	57	4	47	215	7	177	1.23e-69	211
MS.gene50787.t1	MTR_1g085500	46.009	213	108	3	5	215	6	213	2.84e-60	188
MS.gene50787.t1	MTR_1g085140	46.000	200	101	3	18	215	20	214	1.23e-53	171
MS.gene50787.t1	MTR_7g111240	47.449	196	96	3	22	215	20	210	4.29e-52	167
MS.gene50787.t1	MTR_2g031300	54.386	171	53	4	47	214	7	155	1.22e-51	164
MS.gene50787.t1	MTR_7g111240	47.449	196	93	3	22	215	20	207	8.46e-51	164
MS.gene50787.t1	MTR_5g036570	45.685	197	99	4	22	215	27	218	2.29e-49	160
MS.gene50787.t1	MTR_1g112200	41.346	208	90	7	19	215	20	206	2.10e-43	145
MS.gene50787.t1	MTR_2g041550	41.981	212	106	5	4	213	7	203	1.32e-42	143
MS.gene50787.t1	MTR_1g060120	36.652	221	128	5	2	215	5	220	1.12e-41	141
MS.gene50787.t1	MTR_2g041570	38.498	213	114	5	4	214	7	204	7.41e-39	133
MS.gene50787.t1	MTR_2g041580	38.679	212	113	5	4	213	7	203	8.89e-39	133
MS.gene50787.t1	MTR_8g007300	38.532	218	118	5	4	219	11	214	3.71e-36	127
MS.gene50787.t1	MTR_8g020590	40.838	191	104	5	26	214	19	202	1.02e-34	123
MS.gene50787.t1	MTR_8g020750	39.594	197	99	5	1	190	1	184	2.53e-34	122
MS.gene50787.t1	MTR_3g019710	39.378	193	107	5	7	197	3	187	9.07e-33	118
MS.gene50787.t1	MTR_8g020630	38.674	181	98	5	1	179	1	170	1.63e-32	117
MS.gene50787.t1	MTR_2g036120	36.493	211	118	6	1	206	1	200	6.76e-31	113
MS.gene50787.t1	MTR_8g020650	35.938	192	112	5	26	215	22	204	3.12e-30	111
MS.gene50787.t1	MTR_8g020770	40.223	179	95	4	1	176	1	170	3.94e-30	111
MS.gene50787.t1	MTR_8g020670	33.641	217	129	6	1	215	1	204	1.97e-29	109
MS.gene50787.t1	MTR_3g019650	39.655	174	95	5	26	197	21	186	6.36e-29	108
MS.gene50787.t1	MTR_0284s0010	36.493	211	118	6	1	206	1	200	8.88e-29	107
MS.gene50787.t1	MTR_3g019680	36.816	201	108	7	1	197	1	186	1.01e-28	107
MS.gene50787.t1	MTR_2g044040	36.313	179	101	5	1	177	1	168	2.55e-28	106
MS.gene50787.t1	MTR_2g086640	33.641	217	118	7	2	214	7	201	1.11e-27	104
MS.gene50787.t1	MTR_2g045100	36.019	211	119	6	1	206	1	200	1.33e-27	104
MS.gene50787.t1	MTR_4g052770	34.703	219	117	6	1	214	6	203	1.78e-27	104
MS.gene50787.t1	MTR_4g052780	35.784	204	105	6	16	214	21	203	1.95e-27	103
MS.gene50787.t1	MTR_2g086620	41.985	131	70	3	85	214	77	202	2.89e-27	103
MS.gene50787.t1	MTR_3g096330	38.418	177	103	3	5	179	560	732	3.06e-27	109
MS.gene50787.t1	MTR_2g086630	41.221	131	71	3	85	214	77	202	7.60e-27	102
MS.gene50787.t1	MTR_5g031460	33.810	210	125	5	1	206	1	200	1.26e-26	102
MS.gene50787.t1	MTR_8g020610	33.953	215	125	7	1	213	1	200	1.06e-25	99.8
MS.gene50787.t1	MTR_8g020760	33.180	217	132	6	1	215	1	206	4.18e-25	98.2
MS.gene50787.t1	MTR_7g011340	37.097	186	105	5	26	206	22	200	1.48e-24	96.7
MS.gene50787.t1	MTR_4g010340	36.782	174	102	4	43	215	34	200	1.49e-24	96.7
MS.gene50787.t1	MTR_8g020763	36.095	169	99	3	43	206	17	181	8.45e-24	94.4
MS.gene50787.t1	MTR_2g019780	32.420	219	132	7	1	215	1	207	9.96e-24	94.7
MS.gene50787.t1	MTR_3g073170	35.484	186	104	6	27	206	26	201	2.98e-23	93.2
MS.gene50787.t1	MTR_3g019810	33.171	205	115	9	1	202	107	292	1.00e-18	83.2
MS.gene50787.t1	MTR_2g030915	48.684	76	24	1	93	168	1	61	1.19e-17	75.5
MS.gene50787.t1	MTR_4g027415	37.500	128	71	4	26	151	22	142	1.24e-15	71.2
MS.gene50787.t1	MTR_2g072560	36.066	122	69	4	1	120	1	115	6.03e-13	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50787.t1	AT3G05950	65.471	223	69	4	6	221	7	228	8.67e-96	279
MS.gene50787.t1	AT5G39150	64.220	218	75	2	1	215	1	218	7.83e-95	276
MS.gene50787.t1	AT5G39180	64.220	218	75	2	1	215	1	218	1.60e-94	275
MS.gene50787.t1	AT5G39120	64.220	218	75	2	1	215	1	218	3.28e-94	275
MS.gene50787.t1	AT5G39160	64.840	219	72	3	1	215	1	218	1.23e-92	271
MS.gene50787.t1	AT5G39110	64.455	211	73	1	7	215	9	219	1.80e-92	270
MS.gene50787.t1	AT5G39190	64.840	219	72	3	1	215	1	218	1.90e-92	270
MS.gene50787.t1	AT5G39130	63.470	219	75	3	1	215	1	218	6.92e-92	269
MS.gene50787.t1	AT5G39160	62.557	219	73	3	1	215	1	214	6.44e-88	259
MS.gene50787.t1	AT4G14630	60.731	219	82	2	1	215	3	221	1.69e-87	258
MS.gene50787.t1	AT3G04200	60.465	215	82	3	1	213	6	219	4.43e-84	249
MS.gene50787.t1	AT5G39190	58.986	217	66	2	1	215	1	196	1.92e-80	239
MS.gene50787.t1	AT5G39160	58.525	217	67	2	1	215	1	196	6.10e-80	238
MS.gene50787.t1	AT5G38910	59.545	220	82	4	1	215	1	218	1.35e-78	235
MS.gene50787.t1	AT5G38960	60.697	201	75	3	18	215	22	221	8.72e-78	233
MS.gene50787.t1	AT5G38940	60.714	196	74	2	22	215	26	220	1.38e-77	233
MS.gene50787.t1	AT5G38940	60.714	196	74	2	22	215	50	244	2.19e-77	233
MS.gene50787.t1	AT5G38930	61.616	198	73	2	20	215	24	220	2.38e-77	232
MS.gene50787.t1	AT3G04170	55.981	209	86	3	8	214	12	216	7.33e-75	226
MS.gene50787.t1	AT3G04190	56.219	201	85	2	15	213	19	218	7.11e-74	223
MS.gene50787.t1	AT3G04150	54.378	217	93	3	3	214	4	219	7.23e-73	221
MS.gene50787.t1	AT3G04150	51.528	229	94	3	3	214	4	232	4.70e-71	217
MS.gene50787.t1	AT3G04180	54.726	201	88	2	15	213	19	218	8.53e-71	215
MS.gene50787.t1	AT1G02335	46.465	198	99	3	20	215	22	214	7.02e-56	177
MS.gene50787.t1	AT5G38910	48.636	220	69	4	1	215	1	181	4.17e-55	174
MS.gene50787.t1	AT3G62020	46.009	213	106	5	6	215	8	214	6.42e-55	175
MS.gene50787.t1	AT1G09560	45.274	201	103	3	17	215	19	214	2.45e-53	171
MS.gene50787.t1	AT3G05930	46.798	203	99	4	3	202	5	201	2.19e-52	168
MS.gene50787.t1	AT1G18970	46.000	200	99	4	18	215	25	217	3.67e-51	165
MS.gene50787.t1	AT5G26700	46.875	192	88	5	26	213	25	206	1.36e-50	164
MS.gene50787.t1	AT1G18980	45.500	200	100	4	18	215	25	217	6.11e-49	159
MS.gene50787.t1	AT3G62020	46.667	180	90	3	39	215	9	185	2.02e-47	155
MS.gene50787.t1	AT3G10080	40.099	202	112	5	18	215	22	218	3.72e-44	147
MS.gene50787.t1	AT1G10460	41.414	198	108	5	22	214	19	213	3.76e-44	147
MS.gene50787.t1	AT3G10080	40.099	202	112	5	18	215	93	289	1.81e-43	148
MS.gene50787.t1	AT5G20630	41.905	210	110	6	1	208	1	200	4.15e-42	142
MS.gene50787.t1	AT5G61750	41.530	183	102	2	6	186	3	182	7.01e-40	136
MS.gene50787.t1	AT1G72610	41.053	190	103	6	26	213	19	201	3.96e-34	121
MS.gene50787.t1	AT1G74820	38.660	194	110	4	24	215	38	224	4.43e-33	119
MS.gene50787.t1	AT5G38950	54.167	72	33	0	145	216	2	73	1.39e-18	78.2
MS.gene50787.t1	AT5G39100	72.000	50	14	0	7	56	9	58	7.86e-18	74.7

Find 58 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATATACATACCACCATCTT+TGG	0.255130	4.1:+80425149	None:intergenic
TTTCAACTGAATGTGGGATA+TGG	0.310660	4.1:-80424541	MS.gene50787:CDS
CCTTGTTAGCATCTTGGCTT+TGG	0.311083	4.1:-80425238	MS.gene50787:CDS
TTGAGATCCTAGTGCATTAG+AGG	0.333822	4.1:+80424810	None:intergenic
CTTATCCACTTGAAAAGCTT+TGG	0.335788	4.1:+80424414	None:intergenic
TAACACGCTTGGTATATCTT+TGG	0.340502	4.1:-80424752	MS.gene50787:CDS
TGAAAATGAATAAGACCAAT+TGG	0.356438	4.1:+80424559	None:intergenic
ATACTTCCTTGTTAGCATCT+TGG	0.361887	4.1:-80425244	MS.gene50787:CDS
GAGTGCCTTCGAGTACTATA+AGG	0.397511	4.1:+80424665	None:intergenic
TTCTTCAAACATGTTGAAGC+TGG	0.402098	4.1:-80424838	MS.gene50787:CDS
ATATACATACCACCATCTTT+GGG	0.405165	4.1:+80425150	None:intergenic
GGCAACGCTGTTGCTATTGC+TGG	0.409649	4.1:-80424520	MS.gene50787:CDS
CTCTAGGGTGGATGTGAGGT+GGG	0.411548	4.1:+80424698	None:intergenic
GATGTGTTTGTGTTCCCAAT+TGG	0.424230	4.1:-80424574	MS.gene50787:CDS
TAAAGAGCCGGTTGTTGTCT+TGG	0.425026	4.1:+80424617	None:intergenic
CAAAAGTCTTGGAGAGGGCT+AGG	0.434671	4.1:+80425189	None:intergenic
TAATTTGCAGTATTCGTGAA+TGG	0.437869	4.1:-80424901	MS.gene50787:intron
ATTATCTTCAAAAGCAGTCT+TGG	0.442184	4.1:-80424381	MS.gene50787:CDS
CTCGAAGGCACTCTTTATGT+TGG	0.443268	4.1:-80424655	MS.gene50787:CDS
TCTTCAGCTTTAACAAGTGC+GGG	0.444792	4.1:+80424862	None:intergenic
GTCACTGTAGACCAACTATT+CGG	0.446134	4.1:-80424778	MS.gene50787:CDS
CATCAAACCAAGACAACAAC+CGG	0.452231	4.1:-80424624	MS.gene50787:CDS
ATAAGGATCTCTGTTCCTCT+AGG	0.468428	4.1:+80424682	None:intergenic
TTTGCAACGGTGATAACTCC+TGG	0.470922	4.1:+80424481	None:intergenic
TGATTGCAACACAAAAGTCT+TGG	0.490396	4.1:+80425178	None:intergenic
AATTTGCAGTATTCGTGAAT+GGG	0.491761	4.1:-80424900	MS.gene50787:intron
CCTCTAGGGTGGATGTGAGG+TGG	0.496940	4.1:+80424697	None:intergenic
TCTTCAAACATGTTGAAGCT+GGG	0.501315	4.1:-80424837	MS.gene50787:CDS
CTATTCGGACTTAACACGCT+TGG	0.510323	4.1:-80424763	MS.gene50787:CDS
GAAAATGAATAAGACCAATT+GGG	0.510497	4.1:+80424560	None:intergenic
AGAACTTCGTCGGAAATAAG+AGG	0.511108	4.1:+80424439	None:intergenic
AATAGTTGGTCTACAGTGAC+TGG	0.527501	4.1:+80424781	None:intergenic
GCGTGTTAAGTCCGAATAGT+TGG	0.534502	4.1:+80424767	None:intergenic
TGAGGTGGGTTTAAACCCTT+AGG	0.537084	4.1:+80424712	None:intergenic
ATCTTCAGCTTTAACAAGTG+CGG	0.543612	4.1:+80424861	None:intergenic
CTTTACCAAGGTGCTCAACA+AGG	0.544491	4.1:-80424599	MS.gene50787:CDS
GCAATCAAAGACCCCAAAGA+TGG	0.554146	4.1:-80425162	MS.gene50787:CDS
AGCTTTGGTAAGAACTTCGT+CGG	0.555884	4.1:+80424429	None:intergenic
CCAAAGCCAAGATGCTAACA+AGG	0.556137	4.1:+80425238	None:intergenic
AAAAGTCTTGGAGAGGGCTA+GGG	0.564154	4.1:+80425190	None:intergenic
ATCACCCTTGTTGAGCACCT+TGG	0.567836	4.1:+80424594	None:intergenic
TAAGGATCTCTGTTCCTCTA+GGG	0.571738	4.1:+80424683	None:intergenic
GGACTTAGCAGTCAAAATCC+AGG	0.578792	4.1:-80424499	MS.gene50787:CDS
TGAGCACCTTGGTAAAGAGC+CGG	0.579420	4.1:+80424605	None:intergenic
GGGAATGCCTCTAATGCACT+AGG	0.589101	4.1:-80424817	MS.gene50787:CDS
GGATCTCTGTTCCTCTAGGG+TGG	0.590427	4.1:+80424686	None:intergenic
TTTAAACAAAGCATTTGCAA+CGG	0.590900	4.1:+80424468	None:intergenic
AGGGCTAGGGTCATAAGCAA+AGG	0.622370	4.1:+80425203	None:intergenic
ATCAAAGACCCCAAAGATGG+TGG	0.628410	4.1:-80425159	MS.gene50787:intron
TATACATACCACCATCTTTG+GGG	0.639620	4.1:+80425151	None:intergenic
CAACACAAAAGTCTTGGAGA+GGG	0.641422	4.1:+80425184	None:intergenic
GTTCCTCTAGGGTGGATGTG+AGG	0.650118	4.1:+80424694	None:intergenic
GCAACACAAAAGTCTTGGAG+AGG	0.651085	4.1:+80425183	None:intergenic
CCACCTCACATCCACCCTAG+AGG	0.660499	4.1:-80424697	MS.gene50787:CDS
CTTCAGCTTTAACAAGTGCG+GGG	0.662073	4.1:+80424863	None:intergenic
GAGATCCTTATAGTACTCGA+AGG	0.666965	4.1:-80424670	MS.gene50787:CDS
TTTACCAAGGTGCTCAACAA+GGG	0.669433	4.1:-80424598	MS.gene50787:CDS
CAACAACCGGCTCTTTACCA+AGG	0.674974	4.1:-80424611	MS.gene50787:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	CTTCAATAAATATATTTTTA+TGG	-	chr4.1:80424576-80424595	MS.gene50787:CDS	10.0%
!!	TAAAATGTTTGATATTTGTA+AGG	+	chr4.1:80424550-80424569	None:intergenic	15.0%
!!!	TTAATTACTACTCAATTTTT+AGG	-	chr4.1:80424506-80424525	MS.gene50787:CDS	15.0%
!!!	ATGTATAGAATCTTTTGTTT+TGG	-	chr4.1:80424612-80424631	MS.gene50787:CDS	20.0%
!	GAAAATGAATAAGACCAATT+GGG	+	chr4.1:80425064-80425083	None:intergenic	25.0%
!	TGAAAATGAATAAGACCAAT+TGG	+	chr4.1:80425065-80425084	None:intergenic	25.0%
!	TGAGTAGTAATTAAGACTTA+AGG	+	chr4.1:80424500-80424519	None:intergenic	25.0%
!!	ATTCATTTTCAACTGAATGT+GGG	-	chr4.1:80425074-80425093	MS.gene50787:intron	25.0%
!!	TATTCATTTTCAACTGAATG+TGG	-	chr4.1:80425073-80425092	MS.gene50787:intron	25.0%
!!	TTTAAACAAAGCATTTGCAA+CGG	+	chr4.1:80425156-80425175	None:intergenic	25.0%
	AATTTGCAGTATTCGTGAAT+GGG	-	chr4.1:80424721-80424740	MS.gene50787:CDS	30.0%
	ATATACATACCACCATCTTT+GGG	+	chr4.1:80424474-80424493	None:intergenic	30.0%
	ATTATCTTCAAAAGCAGTCT+TGG	-	chr4.1:80425240-80425259	MS.gene50787:CDS	30.0%
	TATATACATACCACCATCTT+TGG	+	chr4.1:80424475-80424494	None:intergenic	30.0%
!	TAATTTGCAGTATTCGTGAA+TGG	-	chr4.1:80424720-80424739	MS.gene50787:CDS	30.0%
	ATACTTCCTTGTTAGCATCT+TGG	-	chr4.1:80424377-80424396	MS.gene50787:CDS	35.0%
	CTTATCCACTTGAAAAGCTT+TGG	+	chr4.1:80425210-80425229	None:intergenic	35.0%
	TAACACGCTTGGTATATCTT+TGG	-	chr4.1:80424869-80424888	MS.gene50787:CDS	35.0%
	TATACATACCACCATCTTTG+GGG	+	chr4.1:80424473-80424492	None:intergenic	35.0%
	TCTTCAAACATGTTGAAGCT+GGG	-	chr4.1:80424784-80424803	MS.gene50787:CDS	35.0%
	TGATTGCAACACAAAAGTCT+TGG	+	chr4.1:80424446-80424465	None:intergenic	35.0%
	TTCTTCAAACATGTTGAAGC+TGG	-	chr4.1:80424783-80424802	MS.gene50787:CDS	35.0%
	TTTCAACTGAATGTGGGATA+TGG	-	chr4.1:80425080-80425099	MS.gene50787:intron	35.0%
!	ATCTTCAGCTTTAACAAGTG+CGG	+	chr4.1:80424763-80424782	None:intergenic	35.0%
!!	TCTTACCAAAGCTTTTCAAG+TGG	-	chr4.1:80425202-80425221	MS.gene50787:CDS	35.0%
	AGAACTTCGTCGGAAATAAG+AGG	+	chr4.1:80425185-80425204	None:intergenic	40.0%
	ATAAGGATCTCTGTTCCTCT+AGG	+	chr4.1:80424942-80424961	None:intergenic	40.0%
	CAACACAAAAGTCTTGGAGA+GGG	+	chr4.1:80424440-80424459	None:intergenic	40.0%
	CATCAAACCAAGACAACAAC+CGG	-	chr4.1:80424997-80425016	MS.gene50787:intron	40.0%
	GAGATCCTTATAGTACTCGA+AGG	-	chr4.1:80424951-80424970	MS.gene50787:intron	40.0%
	GATGTGTTTGTGTTCCCAAT+TGG	-	chr4.1:80425047-80425066	MS.gene50787:intron	40.0%
	GTCACTGTAGACCAACTATT+CGG	-	chr4.1:80424843-80424862	MS.gene50787:CDS	40.0%
	TAAGGATCTCTGTTCCTCTA+GGG	+	chr4.1:80424941-80424960	None:intergenic	40.0%
	TTGAGATCCTAGTGCATTAG+AGG	+	chr4.1:80424814-80424833	None:intergenic	40.0%
!	AATAGTTGGTCTACAGTGAC+TGG	+	chr4.1:80424843-80424862	None:intergenic	40.0%
!	AGCTTTGGTAAGAACTTCGT+CGG	+	chr4.1:80425195-80425214	None:intergenic	40.0%
!	TCTTCAGCTTTAACAAGTGC+GGG	+	chr4.1:80424762-80424781	None:intergenic	40.0%
!	TTTACCAAGGTGCTCAACAA+GGG	-	chr4.1:80425023-80425042	MS.gene50787:intron	40.0%
	AAAAGTCTTGGAGAGGGCTA+GGG	+	chr4.1:80424434-80424453	None:intergenic	45.0%
	AGCTAAACCAGCAATCACTG+AGG	+	chr4.1:80424639-80424658	None:intergenic	45.0%
	ATCAAAGACCCCAAAGATGG+TGG	-	chr4.1:80424462-80424481	MS.gene50787:CDS	45.0%
	CCAAAGCCAAGATGCTAACA+AGG	+	chr4.1:80424386-80424405	None:intergenic	45.0%
	CCTTGTTAGCATCTTGGCTT+TGG	-	chr4.1:80424383-80424402	MS.gene50787:CDS	45.0%
	CTATTCGGACTTAACACGCT+TGG	-	chr4.1:80424858-80424877	MS.gene50787:CDS	45.0%
	GAGTGCCTTCGAGTACTATA+AGG	+	chr4.1:80424959-80424978	None:intergenic	45.0%
	GCAACACAAAAGTCTTGGAG+AGG	+	chr4.1:80424441-80424460	None:intergenic	45.0%
	GCAATCAAAGACCCCAAAGA+TGG	-	chr4.1:80424459-80424478	MS.gene50787:CDS	45.0%
	GCGTGTTAAGTCCGAATAGT+TGG	+	chr4.1:80424857-80424876	None:intergenic	45.0%
	GGACTTAGCAGTCAAAATCC+AGG	-	chr4.1:80425122-80425141	MS.gene50787:intron	45.0%
	TAAAGAGCCGGTTGTTGTCT+TGG	+	chr4.1:80425007-80425026	None:intergenic	45.0%
	TTTGCAACGGTGATAACTCC+TGG	+	chr4.1:80425143-80425162	None:intergenic	45.0%
!	CTTCAGCTTTAACAAGTGCG+GGG	+	chr4.1:80424761-80424780	None:intergenic	45.0%
!	CTTTACCAAGGTGCTCAACA+AGG	-	chr4.1:80425022-80425041	MS.gene50787:intron	45.0%
!	TGAGGTGGGTTTAAACCCTT+AGG	+	chr4.1:80424912-80424931	None:intergenic	45.0%
!	TTTTGGTCCTCAGTGATTGC+TGG	-	chr4.1:80424629-80424648	MS.gene50787:CDS	45.0%
!!	CAGTGATTGCTGGTTTAGCT+AGG	-	chr4.1:80424639-80424658	MS.gene50787:CDS	45.0%
!!	CTCGAAGGCACTCTTTATGT+TGG	-	chr4.1:80424966-80424985	MS.gene50787:intron	45.0%
	AGGGCTAGGGTCATAAGCAA+AGG	+	chr4.1:80424421-80424440	None:intergenic	50.0%
	ATCACCCTTGTTGAGCACCT+TGG	+	chr4.1:80425030-80425049	None:intergenic	50.0%
	CAAAAGTCTTGGAGAGGGCT+AGG	+	chr4.1:80424435-80424454	None:intergenic	50.0%
	GGGAATGCCTCTAATGCACT+AGG	-	chr4.1:80424804-80424823	MS.gene50787:CDS	50.0%
	TGAGCACCTTGGTAAAGAGC+CGG	+	chr4.1:80425019-80425038	None:intergenic	50.0%
!	CAACAACCGGCTCTTTACCA+AGG	-	chr4.1:80425010-80425029	MS.gene50787:intron	50.0%
!!!	CGCGTTGATTTTGCGCCTAA+GGG	-	chr4.1:80424894-80424913	MS.gene50787:CDS	50.0%
!!!	TCGCGTTGATTTTGCGCCTA+AGG	-	chr4.1:80424893-80424912	MS.gene50787:CDS	50.0%
!	GGATCTCTGTTCCTCTAGGG+TGG	+	chr4.1:80424938-80424957	None:intergenic	55.0%
!	GGCAACGCTGTTGCTATTGC+TGG	-	chr4.1:80425101-80425120	MS.gene50787:intron	55.0%
!!	CTCTAGGGTGGATGTGAGGT+GGG	+	chr4.1:80424926-80424945	None:intergenic	55.0%
!!	GTTCCTCTAGGGTGGATGTG+AGG	+	chr4.1:80424930-80424949	None:intergenic	55.0%
	CCACCTCACATCCACCCTAG+AGG	-	chr4.1:80424924-80424943	MS.gene50787:intron	60.0%
!!	CCTCTAGGGTGGATGTGAGG+TGG	+	chr4.1:80424927-80424946	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	80424366	80425277	80424366	ID=MS.gene50787
chr4.1	mRNA	80424366	80425277	80424366	ID=MS.gene50787.t1;Parent=MS.gene50787
chr4.1	exon	80425160	80425277	80425160	ID=MS.gene50787.t1.exon1;Parent=MS.gene50787.t1
chr4.1	CDS	80425160	80425277	80425160	ID=cds.MS.gene50787.t1;Parent=MS.gene50787.t1
chr4.1	exon	80424366	80424913	80424366	ID=MS.gene50787.t1.exon2;Parent=MS.gene50787.t1
chr4.1	CDS	80424366	80424913	80424366	ID=cds.MS.gene50787.t1;Parent=MS.gene50787.t1

Gene Sequence

>MS.gene50787

ATGAAGGTTGTATACTTCCTTGTTAGCATCTTGGCTTTGGCATCATCTGTTGCCTTTGCTTATGACCCTAGCCCTCTCCAAGACTTTTGTGTTGCAATCAAAGACCCCAAAGATGGTGGTATGTATATATTCCTTAAGTCTTAATTACTACTCAATTTTTAGGCTTAAATATACAAATGTTCCTTACAAATATCAAACATTTTAGTTTTACTTCAATAAATATATTTTTATGGTTTTAGTTCTTGTATGTATAGAATCTTTTGTTTTGGTCCTCAGTGATTGCTGGTTTAGCTAGGTCATGACTAGAAGAATTATACTAATGAATTTTTTTTATATAACAAAACTATGATTTGATAATTTGCAGTATTCGTGAATGGGAAATTCTGTAAAGACCCCGCACTTGTTAAAGCTGAAGATTTCTTCAAACATGTTGAAGCTGGGAATGCCTCTAATGCACTAGGATCTCAAGTGACTCCAGTCACTGTAGACCAACTATTCGGACTTAACACGCTTGGTATATCTTTGGCTCGCGTTGATTTTGCGCCTAAGGGTTTAAACCCACCTCACATCCACCCTAGAGGAACAGAGATCCTTATAGTACTCGAAGGCACTCTTTATGTTGGATTTGTCTCATCAAACCAAGACAACAACCGGCTCTTTACCAAGGTGCTCAACAAGGGTGATGTGTTTGTGTTCCCAATTGGTCTTATTCATTTTCAACTGAATGTGGGATATGGCAACGCTGTTGCTATTGCTGGACTTAGCAGTCAAAATCCAGGAGTTATCACCGTTGCAAATGCTTTGTTTAAATCTGATCCTCTTATTTCCGACGAAGTTCTTACCAAAGCTTTTCAAGTGGATAAGAGCATAATTGATTATCTTCAAAAGCAGTCTTGGTATGACAACAACTAG

Protein sequence

>MS.gene50787.t1

MKVVYFLVSILALASSVAFAYDPSPLQDFCVAIKDPKDGVFVNGKFCKDPALVKAEDFFKHVEAGNASNALGSQVTPVTVDQLFGLNTLGISLARVDFAPKGLNPPHIHPRGTEILIVLEGTLYVGFVSSNQDNNRLFTKVLNKGDVFVFPIGLIHFQLNVGYGNAVAIAGLSSQNPGVITVANALFKSDPLISDEVLTKAFQVDKSIIDYLQKQSWYDNN