AlfalfaGEDB | MS.gene50789

M. sativa cultivar XinJiangDaYe / MS.gene50789

KEGG: -
GO: GO:0003674 GO:0005488 GO:0030145 GO:0043167 GO:0043169 GO:0045735 GO:0046872 GO:0046914
PFAM: PF00190.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50789.t1	XP_003604729.1	97.3	222	5	1	1	222	1	221	1.60E-116	428.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50789.t1	Q9SFF9	65.9	217	68	4	10	222	14	228	1.2e-72	274.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50789.t1	G7JQ50	97.3	222	5	1	1	222	1	221	1.2e-116	428.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049549	MS.gene50789	0.813057	3.10E-51	-1.69E-46
MS.gene050380	MS.gene50789	0.800577	1.35E-48	-1.69E-46
MS.gene050809	MS.gene50789	0.811776	5.91E-51	-1.69E-46
MS.gene051158	MS.gene50789	0.800598	1.34E-48	-1.69E-46
MS.gene051385	MS.gene50789	0.815677	8.18E-52	-1.69E-46
MS.gene051632	MS.gene50789	0.800299	1.54E-48	-1.69E-46
MS.gene05240	MS.gene50789	0.834368	3.12E-56	-1.69E-46
MS.gene05244	MS.gene50789	0.812212	4.75E-51	-1.69E-46
MS.gene053030	MS.gene50789	0.808245	3.40E-50	-1.69E-46
MS.gene053031	MS.gene50789	0.815021	1.14E-51	-1.69E-46
MS.gene053033	MS.gene50789	0.820034	8.49E-53	-1.69E-46
MS.gene053406	MS.gene50789	0.836489	9.08E-57	-1.69E-46
MS.gene053420	MS.gene50789	0.808791	2.60E-50	-1.69E-46
MS.gene054290	MS.gene50789	0.847389	1.19E-59	-1.69E-46
MS.gene054742	MS.gene50789	0.819391	1.19E-52	-1.69E-46
MS.gene055240	MS.gene50789	0.810885	9.23E-51	-1.69E-46
MS.gene055959	MS.gene50789	0.802095	6.60E-49	-1.69E-46
MS.gene056330	MS.gene50789	0.803242	3.83E-49	-1.69E-46
MS.gene056590	MS.gene50789	0.806325	8.69E-50	-1.69E-46
MS.gene056591	MS.gene50789	0.804913	1.72E-49	-1.69E-46
MS.gene056688	MS.gene50789	0.803889	2.81E-49	-1.69E-46
MS.gene056920	MS.gene50789	0.802846	4.62E-49	-1.69E-46
MS.gene057544	MS.gene50789	0.838733	2.41E-57	-1.69E-46
MS.gene058093	MS.gene50789	0.818337	2.07E-52	-1.69E-46
MS.gene058177	MS.gene50789	0.811515	6.73E-51	-1.69E-46
MS.gene058179	MS.gene50789	0.805001	1.65E-49	-1.69E-46
MS.gene058180	MS.gene50789	0.801503	8.73E-49	-1.69E-46
MS.gene059037	MS.gene50789	0.812224	4.72E-51	-1.69E-46
MS.gene059402	MS.gene50789	0.808767	2.63E-50	-1.69E-46
MS.gene059955	MS.gene50789	0.809044	2.30E-50	-1.69E-46
MS.gene060087	MS.gene50789	0.810777	9.74E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene82859	MS.gene50789	PPI
MS.gene89775	MS.gene50789	PPI
MS.gene034877	MS.gene50789	PPI
MS.gene82858	MS.gene50789	PPI
MS.gene82855	MS.gene50789	PPI
MS.gene50786	MS.gene50789	PPI
MS.gene053031	MS.gene50789	PPI
MS.gene50789	MS.gene82859	PPI
MS.gene99166	MS.gene50789	PPI
MS.gene022634	MS.gene50789	PPI
MS.gene82860	MS.gene50789	PPI
MS.gene50789	MS.gene07591	PPI
MS.gene81739	MS.gene50789	PPI
MS.gene056870	MS.gene50789	PPI
MS.gene005041	MS.gene50789	PPI
MS.gene70061	MS.gene50789	PPI
MS.gene037516	MS.gene50789	PPI
MS.gene82857	MS.gene50789	PPI
MS.gene50790	MS.gene50789	PPI
MS.gene037519	MS.gene50789	PPI
MS.gene50789	MS.gene053031	PPI
MS.gene89773	MS.gene50789	PPI
MS.gene004411	MS.gene50789	PPI
MS.gene002728	MS.gene50789	PPI
MS.gene037517	MS.gene50789	PPI
MS.gene032596	MS.gene50789	PPI
MS.gene50788	MS.gene50789	PPI
MS.gene70064	MS.gene50789	PPI
MS.gene74887	MS.gene50789	PPI
MS.gene89772	MS.gene50789	PPI
MS.gene50789	MS.gene037520	PPI
MS.gene047464	MS.gene50789	PPI
MS.gene89774	MS.gene50789	PPI
MS.gene50789	MS.gene82855	PPI
MS.gene50789	MS.gene70061	PPI
MS.gene032598	MS.gene50789	PPI
MS.gene70063	MS.gene50789	PPI
MS.gene053033	MS.gene50789	PPI
MS.gene037520	MS.gene50789	PPI
MS.gene053027	MS.gene50789	PPI
MS.gene037851	MS.gene50789	PPI
MS.gene50789	MS.gene037518	PPI
MS.gene50787	MS.gene50789	PPI
MS.gene70062	MS.gene50789	PPI
MS.gene055423	MS.gene50789	PPI
MS.gene032599	MS.gene50789	PPI
MS.gene07591	MS.gene50789	PPI
MS.gene50789	MS.gene047464	PPI
MS.gene032601	MS.gene50789	PPI
MS.gene053029	MS.gene50789	PPI
MS.gene037518	MS.gene50789	PPI
MS.gene053030	MS.gene50789	PPI
MS.gene053026	MS.gene50789	PPI
MS.gene032597	MS.gene50789	PPI
MS.gene50789	MS.gene82857	PPI
MS.gene96244	MS.gene50789	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50789.t1	MTR_5g046410	97.297	222	5	1	1	222	1	221	6.22e-157	433
MS.gene50789.t1	MTR_5g046430	97.297	222	5	1	1	222	1	221	6.22e-157	433
MS.gene50789.t1	MTR_4g017030	97.297	222	5	1	1	222	1	221	6.22e-157	433
MS.gene50789.t1	MTR_4g017050	96.847	222	6	1	1	222	1	221	2.15e-156	432
MS.gene50789.t1	MTR_4g017040	95.495	222	9	1	1	222	1	221	1.10e-154	427
MS.gene50789.t1	MTR_2g019250	95.045	222	10	1	1	222	1	221	1.18e-154	427
MS.gene50789.t1	MTR_1g079490	78.924	223	45	2	1	222	1	222	5.78e-126	355
MS.gene50789.t1	MTR_6g005340	76.682	223	48	3	1	222	1	220	1.34e-117	334
MS.gene50789.t1	MTR_6g005330	76.233	223	49	3	1	222	1	220	2.12e-117	333
MS.gene50789.t1	MTR_6g005360	76.233	223	49	3	1	222	1	220	2.58e-117	333
MS.gene50789.t1	MTR_6g005310	76.577	222	48	3	1	221	1	219	5.04e-117	332
MS.gene50789.t1	MTR_6g005350	62.162	222	81	2	1	222	1	219	2.80e-91	267
MS.gene50789.t1	MTR_6g005380	60.766	209	81	1	1	209	1	208	1.49e-87	258
MS.gene50789.t1	MTR_1g023770	58.716	218	78	3	1	216	1	208	2.59e-80	239
MS.gene50789.t1	MTR_2g030855	59.701	201	70	4	19	216	19	211	1.42e-76	229
MS.gene50789.t1	MTR_2g031270	57.273	220	82	5	1	216	1	212	1.21e-72	220
MS.gene50789.t1	MTR_2g030895	63.218	174	57	4	47	216	7	177	4.36e-70	212
MS.gene50789.t1	MTR_2g030865	62.644	174	58	4	47	216	7	177	3.75e-68	207
MS.gene50789.t1	MTR_1g085500	46.759	216	107	3	3	216	4	213	1.16e-61	192
MS.gene50789.t1	MTR_1g085140	46.269	201	100	3	18	216	20	214	2.61e-54	173
MS.gene50789.t1	MTR_7g111240	47.716	197	95	3	22	216	20	210	8.62e-53	169
MS.gene50789.t1	MTR_7g111240	47.716	197	92	3	22	216	20	207	1.53e-51	166
MS.gene50789.t1	MTR_2g031300	53.488	172	54	4	47	215	7	155	2.21e-50	161
MS.gene50789.t1	MTR_5g036570	45.455	198	99	4	22	216	27	218	1.45e-48	159
MS.gene50789.t1	MTR_1g112200	40.278	216	111	6	3	216	7	206	1.68e-44	148
MS.gene50789.t1	MTR_1g060120	36.486	222	128	5	2	216	5	220	7.83e-42	142
MS.gene50789.t1	MTR_2g041550	40.845	213	108	5	4	214	7	203	1.33e-40	138
MS.gene50789.t1	MTR_2g041570	38.140	215	115	5	3	215	6	204	3.09e-39	134
MS.gene50789.t1	MTR_2g041580	38.318	214	114	5	3	214	6	203	4.00e-39	134
MS.gene50789.t1	MTR_8g007300	40.299	201	106	4	22	220	26	214	1.97e-36	127
MS.gene50789.t1	MTR_8g020750	40.909	198	96	5	1	191	1	184	1.64e-35	125
MS.gene50789.t1	MTR_8g020590	38.249	217	117	6	1	215	1	202	8.89e-34	120
MS.gene50789.t1	MTR_3g019710	39.691	194	106	5	7	198	3	187	2.45e-33	119
MS.gene50789.t1	MTR_8g020630	40.217	184	92	6	1	180	1	170	6.42e-33	118
MS.gene50789.t1	MTR_8g020770	41.667	180	92	4	1	177	1	170	2.53e-31	114
MS.gene50789.t1	MTR_2g036120	37.264	212	116	6	1	207	1	200	2.58e-31	114
MS.gene50789.t1	MTR_8g020650	36.269	193	111	5	26	216	22	204	6.46e-31	113
MS.gene50789.t1	MTR_8g020670	34.419	215	123	6	4	216	6	204	5.59e-30	110
MS.gene50789.t1	MTR_3g019680	37.563	197	105	6	4	198	6	186	7.75e-30	110
MS.gene50789.t1	MTR_3g019650	37.563	197	105	6	4	198	6	186	8.44e-30	110
MS.gene50789.t1	MTR_0284s0010	37.850	214	112	7	1	207	1	200	3.89e-29	108
MS.gene50789.t1	MTR_2g044040	37.222	180	99	5	1	178	1	168	8.38e-29	107
MS.gene50789.t1	MTR_2g086620	43.182	132	68	3	85	215	77	202	1.62e-28	107
MS.gene50789.t1	MTR_4g052780	36.585	205	103	6	16	215	21	203	1.77e-28	107
MS.gene50789.t1	MTR_2g086640	34.404	218	116	7	2	215	7	201	1.86e-28	106
MS.gene50789.t1	MTR_2g045100	38.983	177	92	5	4	178	6	168	2.95e-28	106
MS.gene50789.t1	MTR_4g052770	36.098	205	104	6	16	215	21	203	3.51e-28	106
MS.gene50789.t1	MTR_2g086630	42.424	132	69	3	85	215	77	202	5.41e-28	105
MS.gene50789.t1	MTR_3g096330	38.333	180	104	3	3	180	558	732	7.06e-28	111
MS.gene50789.t1	MTR_5g031460	34.597	211	123	5	1	207	1	200	6.10e-27	102
MS.gene50789.t1	MTR_8g020760	34.862	218	128	6	1	216	1	206	9.97e-26	99.8
MS.gene50789.t1	MTR_4g010340	35.000	220	118	7	1	216	2	200	1.09e-25	99.8
MS.gene50789.t1	MTR_7g011340	37.433	187	104	5	26	207	22	200	2.95e-25	98.6
MS.gene50789.t1	MTR_8g020763	36.471	170	98	3	43	207	17	181	1.56e-24	96.3
MS.gene50789.t1	MTR_8g020610	33.796	216	125	7	1	214	1	200	3.42e-24	95.9
MS.gene50789.t1	MTR_2g019780	32.727	220	131	7	1	216	1	207	3.63e-24	95.9
MS.gene50789.t1	MTR_3g073170	34.896	192	109	6	28	216	27	205	5.36e-24	95.1
MS.gene50789.t1	MTR_2g030915	48.052	77	24	1	93	169	1	61	9.65e-18	75.9
MS.gene50789.t1	MTR_3g019810	33.495	206	114	9	1	203	107	292	9.88e-18	80.5
MS.gene50789.t1	MTR_4g027415	37.984	129	70	4	26	152	22	142	1.81e-16	73.6
MS.gene50789.t1	MTR_2g072560	36.066	122	69	4	1	120	1	115	1.12e-12	63.5
MS.gene50789.t1	MTR_3g019610	37.736	106	61	3	7	110	3	105	5.31e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50789.t1	AT3G05950	65.899	217	68	4	10	222	14	228	1.71e-94	276
MS.gene50789.t1	AT5G39150	62.037	216	79	2	3	216	4	218	3.13e-92	270
MS.gene50789.t1	AT5G39180	62.037	216	79	2	3	216	4	218	7.42e-92	269
MS.gene50789.t1	AT5G39120	63.679	212	73	3	7	216	9	218	1.34e-91	268
MS.gene50789.t1	AT5G39160	64.455	211	71	3	8	216	10	218	3.56e-90	265
MS.gene50789.t1	AT5G39110	63.208	212	75	2	7	216	9	219	5.77e-90	264
MS.gene50789.t1	AT5G39190	64.455	211	71	3	8	216	10	218	6.72e-90	264
MS.gene50789.t1	AT5G39130	63.507	211	73	3	8	216	10	218	2.05e-89	263
MS.gene50789.t1	AT4G14630	60.000	220	83	3	1	216	3	221	3.14e-86	254
MS.gene50789.t1	AT5G39160	62.085	211	72	3	8	216	10	214	3.18e-85	252
MS.gene50789.t1	AT3G04200	61.836	207	75	4	10	214	15	219	7.23e-82	244
MS.gene50789.t1	AT5G38910	59.729	221	81	4	1	216	1	218	6.37e-80	239
MS.gene50789.t1	AT5G39190	58.373	209	65	2	8	216	10	196	1.15e-77	232
MS.gene50789.t1	AT5G38930	61.307	199	73	2	20	216	24	220	1.39e-77	233
MS.gene50789.t1	AT5G38940	60.406	197	74	2	22	216	26	220	1.39e-77	233
MS.gene50789.t1	AT5G38940	60.406	197	74	2	22	216	50	244	2.13e-77	233
MS.gene50789.t1	AT5G39160	57.895	209	66	2	8	216	10	196	2.47e-77	231
MS.gene50789.t1	AT5G38960	59.406	202	77	3	18	216	22	221	3.62e-77	231
MS.gene50789.t1	AT3G04170	55.769	208	85	4	10	215	14	216	8.75e-73	221
MS.gene50789.t1	AT3G04190	55.446	202	86	3	15	214	19	218	2.94e-72	219
MS.gene50789.t1	AT3G04150	54.808	208	90	3	10	215	14	219	7.49e-72	218
MS.gene50789.t1	AT3G04150	51.818	220	91	3	10	215	14	232	6.38e-70	214
MS.gene50789.t1	AT3G04180	53.960	202	89	3	15	214	19	218	2.90e-69	211
MS.gene50789.t1	AT1G02335	46.734	199	98	3	20	216	22	214	1.10e-56	179
MS.gene50789.t1	AT5G38910	48.869	221	68	4	1	216	1	181	2.38e-56	177
MS.gene50789.t1	AT3G62020	46.262	214	105	5	6	216	8	214	7.33e-56	177
MS.gene50789.t1	AT1G09560	45.050	202	103	3	17	216	19	214	1.99e-53	171
MS.gene50789.t1	AT3G05930	47.291	203	97	4	4	203	6	201	6.72e-53	170
MS.gene50789.t1	AT5G26700	47.150	193	87	5	26	214	25	206	3.05e-51	165
MS.gene50789.t1	AT1G18970	45.274	201	100	4	18	216	25	217	7.48e-50	162
MS.gene50789.t1	AT1G18980	45.771	201	99	4	18	216	25	217	1.33e-48	159
MS.gene50789.t1	AT3G62020	46.961	181	89	3	39	216	9	185	4.84e-48	156
MS.gene50789.t1	AT1G10460	41.709	199	107	5	22	215	19	213	5.61e-45	149
MS.gene50789.t1	AT3G10080	39.409	203	113	5	18	216	22	218	1.94e-43	146
MS.gene50789.t1	AT3G10080	39.409	203	113	5	18	216	93	289	8.37e-43	146
MS.gene50789.t1	AT5G20630	40.758	211	112	6	1	209	1	200	4.63e-40	137
MS.gene50789.t1	AT5G61750	40.860	186	104	2	4	187	1	182	5.53e-40	136
MS.gene50789.t1	AT1G74820	39.175	194	108	4	25	216	39	224	7.54e-33	119
MS.gene50789.t1	AT1G72610	40.314	191	104	6	26	214	19	201	8.57e-33	118
MS.gene50789.t1	AT5G38950	54.167	72	33	0	146	217	2	73	1.22e-18	78.2
MS.gene50789.t1	AT5G39100	72.000	50	14	0	7	56	9	58	1.19e-17	74.3

Find 57 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATATACATACCACCATCTT+TGG	0.255130	4.1:-80379661	None:intergenic
TTTCAACTGAATGTTGGATA+TGG	0.274546	4.1:+80380233	MS.gene50789:CDS
TAACACTCTTGGTATATCTT+TGG	0.286436	4.1:+80380019	MS.gene50789:CDS
TTGAGAGCCTAGTGCGTTAG+AGG	0.314204	4.1:-80379961	None:intergenic
CCTTCTTAGCATCCTGGCTT+TGG	0.315409	4.1:+80379572	MS.gene50789:CDS
CTTATCCACTTGAAAAGCTT+TGG	0.335788	4.1:-80380360	None:intergenic
TGAAAATGAATAAGACCAAT+TGG	0.356438	4.1:-80380215	None:intergenic
GACTGCCTTCAAGTACTATA+AGG	0.359669	4.1:-80380106	None:intergenic
TTCTTCAAACATGTTGAAGC+TGG	0.402098	4.1:+80379933	MS.gene50789:CDS
ATATACATACCACCATCTTT+GGG	0.405165	4.1:-80379660	None:intergenic
CTCTAGGGTGGATGTGAGGT+GGG	0.411548	4.1:-80380073	None:intergenic
TTGGAGAGGGCTAGGGTCAT+AGG	0.420416	4.1:-80379613	None:intergenic
GATGTGTTTGTGTTCCCAAT+TGG	0.424230	4.1:+80380200	MS.gene50789:CDS
GGCAACGCGGTTGCTATCGC+TGG	0.429748	4.1:+80380254	MS.gene50789:CDS
CAAAAGTCTTGGAGAGGGCT+AGG	0.434671	4.1:-80379621	None:intergenic
ATTATCTTCAAAAGCAGTCT+TGG	0.442184	4.1:+80380393	MS.gene50789:CDS
TCTTCAGCTTTAACAAGTGC+GGG	0.444792	4.1:-80379909	None:intergenic
GTCACTGTAGACCAACTATT+CGG	0.446134	4.1:+80379993	MS.gene50789:CDS
GAGATCCTTATAGTACTTGA+AGG	0.457154	4.1:+80380101	MS.gene50789:CDS
TACTTTGCAGTATTTGTGAA+TGG	0.463825	4.1:+80379870	MS.gene50789:intron
ATAAGGATCTCTGTTCCTCT+AGG	0.468428	4.1:-80380089	None:intergenic
TTTGCAACGGTGATAACTCC+TGG	0.470922	4.1:-80380293	None:intergenic
ATACTTCCTTCTTAGCATCC+TGG	0.477756	4.1:+80379566	MS.gene50789:CDS
CCAAAGCCAGGATGCTAAGA+AGG	0.479865	4.1:-80379572	None:intergenic
CTATTCGGACTTAACACTCT+TGG	0.480493	4.1:+80380008	MS.gene50789:CDS
CTTGAAGGCAGTCTTTATGT+TGG	0.489113	4.1:+80380116	MS.gene50789:CDS
TGATTGCAACACAAAAGTCT+TGG	0.490396	4.1:-80379632	None:intergenic
CCTCTAGGGTGGATGTGAGG+TGG	0.496940	4.1:-80380074	None:intergenic
TCTTCAAACATGTTGAAGCT+GGG	0.501315	4.1:+80379934	MS.gene50789:CDS
CAACAGAGGATGCCAAAGCC+AGG	0.505056	4.1:-80379584	None:intergenic
GAAAATGAATAAGACCAATT+GGG	0.510497	4.1:-80380214	None:intergenic
AGAACTTCGTCGGAAATAAG+AGG	0.511108	4.1:-80380335	None:intergenic
GAGTGTTAAGTCCGAATAGT+TGG	0.535207	4.1:-80380004	None:intergenic
TGAGGTGGGTTTAAACCCTT+AGG	0.537084	4.1:-80380059	None:intergenic
ATCTTCAGCTTTAACAAGTG+CGG	0.543612	4.1:-80379910	None:intergenic
CTTTACCAAGGTGCTCAACA+AGG	0.544491	4.1:+80380175	MS.gene50789:CDS
GCAATCAAAGACCCCAAAGA+TGG	0.554146	4.1:+80379648	MS.gene50789:CDS
AGCTTTGGTAAGAACTTCGT+CGG	0.555884	4.1:-80380345	None:intergenic
AAAAGTCTTGGAGAGGGCTA+GGG	0.564154	4.1:-80379620	None:intergenic
ATCACCCTTGTTGAGCACCT+TGG	0.567836	4.1:-80380180	None:intergenic
TAAGGATCTCTGTTCCTCTA+GGG	0.571738	4.1:-80380088	None:intergenic
GGGAATGCCTCTAACGCACT+AGG	0.573570	4.1:+80379954	MS.gene50789:CDS
GGACTTAGCAGTCAAAATCC+AGG	0.578792	4.1:+80380275	MS.gene50789:CDS
GGATCTCTGTTCCTCTAGGG+TGG	0.590427	4.1:-80380085	None:intergenic
TTTAAACAAAGCATTTGCAA+CGG	0.590900	4.1:-80380306	None:intergenic
AGGGCTAGGGTCATAGGCAA+AGG	0.609226	4.1:-80379607	None:intergenic
ATCAAAGACCCCAAAGATGG+TGG	0.628410	4.1:+80379651	MS.gene50789:CDS
GTCATAGGCAAAGGCAACAG+AGG	0.637895	4.1:-80379598	None:intergenic
TATACATACCACCATCTTTG+GGG	0.639620	4.1:-80379659	None:intergenic
CAACACAAAAGTCTTGGAGA+GGG	0.641422	4.1:-80379626	None:intergenic
GTTCCTCTAGGGTGGATGTG+AGG	0.650118	4.1:-80380077	None:intergenic
GCAACACAAAAGTCTTGGAG+AGG	0.651085	4.1:-80379627	None:intergenic
TTTACCAAGGTGCTCAACAA+GGG	0.659466	4.1:+80380176	MS.gene50789:CDS
CCACCTCACATCCACCCTAG+AGG	0.660499	4.1:+80380074	MS.gene50789:CDS
CTTCAGCTTTAACAAGTGCG+GGG	0.662073	4.1:-80379908	None:intergenic
CAATAATCGTCTCTTTACCA+AGG	0.672342	4.1:+80380163	MS.gene50789:CDS
GAATGTTGGATATGGCAACG+CGG	0.840401	4.1:+80380241	MS.gene50789:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAATATTTTTTAATTTTTTT+TGG	+	chr4.1:80379781-80379800	MS.gene50789:intron	0.0%
!!!	AAATTAAAAAATATTTTTAT+AGG	-	chr4.1:80379777-80379796	None:intergenic	0.0%
!!!	AATTAAAAAATATTTTTATA+GGG	-	chr4.1:80379776-80379795	None:intergenic	0.0%
!!	TCTACAAATATTAGACTTAT+TGG	+	chr4.1:80379743-80379762	MS.gene50789:intron	20.0%
!	AACCAAAACAAAAGATTACA+TGG	-	chr4.1:80379814-80379833	None:intergenic	25.0%
!	AATCATAGTTTAGTTAAACC+AGG	-	chr4.1:80379847-80379866	None:intergenic	25.0%
!	GAAAATGAATAAGACCAATT+GGG	-	chr4.1:80380217-80380236	None:intergenic	25.0%
!	GTAATCAAGACTTAAAGTTA+AGG	-	chr4.1:80379689-80379708	None:intergenic	25.0%
!	TGAAAATGAATAAGACCAAT+TGG	-	chr4.1:80380218-80380237	None:intergenic	25.0%
!!	ATTCATTTTCAACTGAATGT+TGG	+	chr4.1:80380227-80380246	MS.gene50789:CDS	25.0%
!!	TTTAAACAAAGCATTTGCAA+CGG	-	chr4.1:80380309-80380328	None:intergenic	25.0%
!!!	TAATCTTTTGTTTTGGTTCA+TGG	+	chr4.1:80379816-80379835	MS.gene50789:intron	25.0%
	ATATACATACCACCATCTTT+GGG	-	chr4.1:80379663-80379682	None:intergenic	30.0%
	ATTATCTTCAAAAGCAGTCT+TGG	+	chr4.1:80380393-80380412	MS.gene50789:CDS	30.0%
	TAACACTCTTGGTATATCTT+TGG	+	chr4.1:80380019-80380038	MS.gene50789:CDS	30.0%
	TACTTTGCAGTATTTGTGAA+TGG	+	chr4.1:80379870-80379889	MS.gene50789:intron	30.0%
	TATATACATACCACCATCTT+TGG	-	chr4.1:80379664-80379683	None:intergenic	30.0%
	TTTCAACTGAATGTTGGATA+TGG	+	chr4.1:80380233-80380252	MS.gene50789:CDS	30.0%
!!	GTCCATGTAATCTTTTGTTT+TGG	+	chr4.1:80379809-80379828	MS.gene50789:intron	30.0%
	CAATAATCGTCTCTTTACCA+AGG	+	chr4.1:80380163-80380182	MS.gene50789:CDS	35.0%
	CTTATCCACTTGAAAAGCTT+TGG	-	chr4.1:80380363-80380382	None:intergenic	35.0%
	GAGATCCTTATAGTACTTGA+AGG	+	chr4.1:80380101-80380120	MS.gene50789:CDS	35.0%
	TATACATACCACCATCTTTG+GGG	-	chr4.1:80379662-80379681	None:intergenic	35.0%
	TCTTCAAACATGTTGAAGCT+GGG	+	chr4.1:80379934-80379953	MS.gene50789:CDS	35.0%
	TGATTGCAACACAAAAGTCT+TGG	-	chr4.1:80379635-80379654	None:intergenic	35.0%
	TTCTTCAAACATGTTGAAGC+TGG	+	chr4.1:80379933-80379952	MS.gene50789:CDS	35.0%
!	ATCTTCAGCTTTAACAAGTG+CGG	-	chr4.1:80379913-80379932	None:intergenic	35.0%
!!	TCTTACCAAAGCTTTTCAAG+TGG	+	chr4.1:80380355-80380374	MS.gene50789:CDS	35.0%
	AGAACTTCGTCGGAAATAAG+AGG	-	chr4.1:80380338-80380357	None:intergenic	40.0%
	ATAAGGATCTCTGTTCCTCT+AGG	-	chr4.1:80380092-80380111	None:intergenic	40.0%
	ATACTTCCTTCTTAGCATCC+TGG	+	chr4.1:80379566-80379585	MS.gene50789:CDS	40.0%
	CAACACAAAAGTCTTGGAGA+GGG	-	chr4.1:80379629-80379648	None:intergenic	40.0%
	CTATTCGGACTTAACACTCT+TGG	+	chr4.1:80380008-80380027	MS.gene50789:CDS	40.0%
	CTTGAAGGCAGTCTTTATGT+TGG	+	chr4.1:80380116-80380135	MS.gene50789:CDS	40.0%
	GACTGCCTTCAAGTACTATA+AGG	-	chr4.1:80380109-80380128	None:intergenic	40.0%
	GAGTGTTAAGTCCGAATAGT+TGG	-	chr4.1:80380007-80380026	None:intergenic	40.0%
	GATGTGTTTGTGTTCCCAAT+TGG	+	chr4.1:80380200-80380219	MS.gene50789:CDS	40.0%
	GTCACTGTAGACCAACTATT+CGG	+	chr4.1:80379993-80380012	MS.gene50789:CDS	40.0%
	TAAGGATCTCTGTTCCTCTA+GGG	-	chr4.1:80380091-80380110	None:intergenic	40.0%
!	AGCTTTGGTAAGAACTTCGT+CGG	-	chr4.1:80380348-80380367	None:intergenic	40.0%
!	TCTTCAGCTTTAACAAGTGC+GGG	-	chr4.1:80379912-80379931	None:intergenic	40.0%
!	TTTACCAAGGTGCTCAACAA+GGG	+	chr4.1:80380176-80380195	MS.gene50789:CDS	40.0%
!!!	TTTTGGTTCATGGTGTTTCC+TGG	+	chr4.1:80379826-80379845	MS.gene50789:intron	40.0%
	AAAAGTCTTGGAGAGGGCTA+GGG	-	chr4.1:80379623-80379642	None:intergenic	45.0%
	ATCAAAGACCCCAAAGATGG+TGG	+	chr4.1:80379651-80379670	MS.gene50789:CDS	45.0%
	GAATGTTGGATATGGCAACG+CGG	+	chr4.1:80380241-80380260	MS.gene50789:CDS	45.0%
	GCAACACAAAAGTCTTGGAG+AGG	-	chr4.1:80379630-80379649	None:intergenic	45.0%
	GCAATCAAAGACCCCAAAGA+TGG	+	chr4.1:80379648-80379667	MS.gene50789:CDS	45.0%
	GGACTTAGCAGTCAAAATCC+AGG	+	chr4.1:80380275-80380294	MS.gene50789:CDS	45.0%
	TTTGCAACGGTGATAACTCC+TGG	-	chr4.1:80380296-80380315	None:intergenic	45.0%
!	CTTCAGCTTTAACAAGTGCG+GGG	-	chr4.1:80379911-80379930	None:intergenic	45.0%
!	CTTTACCAAGGTGCTCAACA+AGG	+	chr4.1:80380175-80380194	MS.gene50789:CDS	45.0%
!	TGAGGTGGGTTTAAACCCTT+AGG	-	chr4.1:80380062-80380081	None:intergenic	45.0%
	ATCACCCTTGTTGAGCACCT+TGG	-	chr4.1:80380183-80380202	None:intergenic	50.0%
	CAAAAGTCTTGGAGAGGGCT+AGG	-	chr4.1:80379624-80379643	None:intergenic	50.0%
	CCAAAGCCAGGATGCTAAGA+AGG	-	chr4.1:80379575-80379594	None:intergenic	50.0%
	CCTTCTTAGCATCCTGGCTT+TGG	+	chr4.1:80379572-80379591	MS.gene50789:CDS	50.0%
	GTCATAGGCAAAGGCAACAG+AGG	-	chr4.1:80379601-80379620	None:intergenic	50.0%
	TTGAGAGCCTAGTGCGTTAG+AGG	-	chr4.1:80379964-80379983	None:intergenic	50.0%
!!!	CGCGTTGATTTTGCGCCTAA+GGG	+	chr4.1:80380044-80380063	MS.gene50789:CDS	50.0%
!!!	TCGCGTTGATTTTGCGCCTA+AGG	+	chr4.1:80380043-80380062	MS.gene50789:CDS	50.0%
	AGGGCTAGGGTCATAGGCAA+AGG	-	chr4.1:80379610-80379629	None:intergenic	55.0%
	CAACAGAGGATGCCAAAGCC+AGG	-	chr4.1:80379587-80379606	None:intergenic	55.0%
	GGGAATGCCTCTAACGCACT+AGG	+	chr4.1:80379954-80379973	MS.gene50789:CDS	55.0%
	TTGGAGAGGGCTAGGGTCAT+AGG	-	chr4.1:80379616-80379635	None:intergenic	55.0%
!	GGATCTCTGTTCCTCTAGGG+TGG	-	chr4.1:80380088-80380107	None:intergenic	55.0%
!!	CTCTAGGGTGGATGTGAGGT+GGG	-	chr4.1:80380076-80380095	None:intergenic	55.0%
!!	GTTCCTCTAGGGTGGATGTG+AGG	-	chr4.1:80380080-80380099	None:intergenic	55.0%
	CCACCTCACATCCACCCTAG+AGG	+	chr4.1:80380074-80380093	MS.gene50789:CDS	60.0%
!!	CCTCTAGGGTGGATGTGAGG+TGG	-	chr4.1:80380077-80380096	None:intergenic	60.0%
	GGCAACGCGGTTGCTATCGC+TGG	+	chr4.1:80380254-80380273	MS.gene50789:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	80379555	80380430	80379555	ID=MS.gene50789
chr4.1	mRNA	80379555	80380430	80379555	ID=MS.gene50789.t1;Parent=MS.gene50789
chr4.1	exon	80379555	80379672	80379555	ID=MS.gene50789.t1.exon1;Parent=MS.gene50789.t1
chr4.1	CDS	80379555	80379672	80379555	ID=cds.MS.gene50789.t1;Parent=MS.gene50789.t1
chr4.1	exon	80379880	80380430	80379880	ID=MS.gene50789.t1.exon2;Parent=MS.gene50789.t1
chr4.1	CDS	80379880	80380430	80379880	ID=cds.MS.gene50789.t1;Parent=MS.gene50789.t1

Gene Sequence

>MS.gene50789

ATGAAAACTATATACTTCCTTCTTAGCATCCTGGCTTTGGCATCCTCTGTTGCCTTTGCCTATGACCCTAGCCCTCTCCAAGACTTTTGTGTTGCAATCAAAGACCCCAAAGATGGTGGTATGTATATATTCCTTAACTTTAAGTCTTGATTACTACTCAATTTTTAAGCTAACATATGAAAATGTTCTCTACAAATATTAGACTTATTGGTTTTAATCCCTATAAAAATATTTTTTAATTTTTTTTGGTTTTAGTCCATGTAATCTTTTGTTTTGGTTCATGGTGTTTCCTGGTTTAACTAAACTATGATTTGATACTTTGCAGTATTTGTGAATGGAAAATTCTGTAAAGACCCCGCACTTGTTAAAGCTGAAGATTTCTTCAAACATGTTGAAGCTGGGAATGCCTCTAACGCACTAGGCTCTCAAGTAACTCAAGTCACTGTAGACCAACTATTCGGACTTAACACTCTTGGTATATCTTTGGCTCGCGTTGATTTTGCGCCTAAGGGTTTAAACCCACCTCACATCCACCCTAGAGGAACAGAGATCCTTATAGTACTTGAAGGCAGTCTTTATGTTGGATTTGTCACGTCAAATCAAGACAACAATAATCGTCTCTTTACCAAGGTGCTCAACAAGGGTGATGTGTTTGTGTTCCCAATTGGTCTTATTCATTTTCAACTGAATGTTGGATATGGCAACGCGGTTGCTATCGCTGGACTTAGCAGTCAAAATCCAGGAGTTATCACCGTTGCAAATGCTTTGTTTAAATCTGATCCTCTTATTTCCGACGAAGTTCTTACCAAAGCTTTTCAAGTGGATAAGAGCATAATTGATTATCTTCAAAAGCAGTCTTGGTATGACAACAACTAG

Protein sequence

>MS.gene50789.t1

MKTIYFLLSILALASSVAFAYDPSPLQDFCVAIKDPKDGVFVNGKFCKDPALVKAEDFFKHVEAGNASNALGSQVTQVTVDQLFGLNTLGISLARVDFAPKGLNPPHIHPRGTEILIVLEGSLYVGFVTSNQDNNNRLFTKVLNKGDVFVFPIGLIHFQLNVGYGNAVAIAGLSSQNPGVITVANALFKSDPLISDEVLTKAFQVDKSIIDYLQKQSWYDNN