AlfalfaGEDB | MS.gene70061

M. sativa cultivar XinJiangDaYe / MS.gene70061

KEGG: -
GO: GO:0003674 GO:0005488 GO:0030145 GO:0043167 GO:0043169 GO:0045735 GO:0046872 GO:0046914
PFAM: PF00190.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70061.t1	XP_003604729.1	97.7	222	4	1	1	222	1	221	3.30E-117	431

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70061.t1	Q9SFF9	65.6	224	68	5	6	222	7	228	5.6e-73	275.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70061.t1	G7JQ50	97.7	222	4	1	1	222	1	221	2.4e-117	431.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049549	MS.gene70061	0.817797	2.74E-52	-1.69E-46
MS.gene050095	MS.gene70061	0.828023	1.13E-54	-1.69E-46
MS.gene050096	MS.gene70061	0.83718	6.05E-57	-1.69E-46
MS.gene050380	MS.gene70061	0.803334	3.66E-49	-1.69E-46
MS.gene050381	MS.gene70061	0.808146	3.57E-50	-1.69E-46
MS.gene050809	MS.gene70061	0.803896	2.80E-49	-1.69E-46
MS.gene05104	MS.gene70061	0.828614	8.15E-55	-1.69E-46
MS.gene051907	MS.gene70061	0.809895	1.51E-50	-1.69E-46
MS.gene052211	MS.gene70061	0.802412	5.68E-49	-1.69E-46
MS.gene05240	MS.gene70061	0.836789	7.62E-57	-1.69E-46
MS.gene05244	MS.gene70061	0.812883	3.39E-51	-1.69E-46
MS.gene052805	MS.gene70061	0.802479	5.50E-49	-1.69E-46
MS.gene053030	MS.gene70061	0.811354	7.30E-51	-1.69E-46
MS.gene053031	MS.gene70061	0.842903	1.95E-58	-1.69E-46
MS.gene053033	MS.gene70061	0.854468	1.21E-61	-1.69E-46
MS.gene053406	MS.gene70061	0.830204	3.35E-55	-1.69E-46
MS.gene053420	MS.gene70061	0.809715	1.65E-50	-1.69E-46
MS.gene054146	MS.gene70061	0.821401	4.12E-53	-1.69E-46
MS.gene054181	MS.gene70061	0.800649	1.31E-48	-1.69E-46
MS.gene054290	MS.gene70061	0.835914	1.27E-56	-1.69E-46
MS.gene054742	MS.gene70061	0.813732	2.20E-51	-1.69E-46
MS.gene055124	MS.gene70061	0.814042	1.88E-51	-1.69E-46
MS.gene055240	MS.gene70061	0.803786	2.95E-49	-1.69E-46
MS.gene056313	MS.gene70061	0.801032	1.09E-48	-1.69E-46
MS.gene056856	MS.gene70061	0.810835	9.46E-51	-1.69E-46
MS.gene057261	MS.gene70061	0.80332	3.69E-49	-1.69E-46
MS.gene057544	MS.gene70061	0.838104	3.51E-57	-1.69E-46
MS.gene057547	MS.gene70061	0.821171	4.65E-53	-1.69E-46
MS.gene058179	MS.gene70061	0.805451	1.33E-49	-1.69E-46
MS.gene058218	MS.gene70061	0.804213	2.41E-49	-1.69E-46
MS.gene058603	MS.gene70061	0.806989	6.29E-50	-1.69E-46
MS.gene059037	MS.gene70061	0.821032	5.01E-53	-1.69E-46
MS.gene059944	MS.gene70061	0.825452	4.65E-54	-1.69E-46
MS.gene059955	MS.gene70061	0.822676	2.09E-53	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene07591	MS.gene70061	PPI
MS.gene70061	MS.gene053031	PPI
MS.gene032601	MS.gene70061	PPI
MS.gene053029	MS.gene70061	PPI
MS.gene037518	MS.gene70061	PPI
MS.gene053030	MS.gene70061	PPI
MS.gene70061	MS.gene07591	PPI
MS.gene053026	MS.gene70061	PPI
MS.gene032597	MS.gene70061	PPI
MS.gene70061	MS.gene037520	PPI
MS.gene96244	MS.gene70061	PPI
MS.gene82859	MS.gene70061	PPI
MS.gene89775	MS.gene70061	PPI
MS.gene034877	MS.gene70061	PPI
MS.gene70061	MS.gene82855	PPI
MS.gene82858	MS.gene70061	PPI
MS.gene82855	MS.gene70061	PPI
MS.gene50786	MS.gene70061	PPI
MS.gene053031	MS.gene70061	PPI
MS.gene70061	MS.gene50789	PPI
MS.gene70061	MS.gene037518	PPI
MS.gene99166	MS.gene70061	PPI
MS.gene022634	MS.gene70061	PPI
MS.gene82860	MS.gene70061	PPI
MS.gene81739	MS.gene70061	PPI
MS.gene056870	MS.gene70061	PPI
MS.gene005041	MS.gene70061	PPI
MS.gene70061	MS.gene047464	PPI
MS.gene82857	MS.gene70061	PPI
MS.gene50790	MS.gene70061	PPI
MS.gene037519	MS.gene70061	PPI
MS.gene70061	MS.gene82857	PPI
MS.gene89773	MS.gene70061	PPI
MS.gene004411	MS.gene70061	PPI
MS.gene002728	MS.gene70061	PPI
MS.gene037517	MS.gene70061	PPI
MS.gene032596	MS.gene70061	PPI
MS.gene50789	MS.gene70061	PPI
MS.gene50788	MS.gene70061	PPI
MS.gene70064	MS.gene70061	PPI
MS.gene74887	MS.gene70061	PPI
MS.gene89772	MS.gene70061	PPI
MS.gene047464	MS.gene70061	PPI
MS.gene89774	MS.gene70061	PPI
MS.gene70061	MS.gene82859	PPI
MS.gene037516	MS.gene70061	PPI
MS.gene032598	MS.gene70061	PPI
MS.gene70063	MS.gene70061	PPI
MS.gene053033	MS.gene70061	PPI
MS.gene037520	MS.gene70061	PPI
MS.gene053027	MS.gene70061	PPI
MS.gene037851	MS.gene70061	PPI
MS.gene50787	MS.gene70061	PPI
MS.gene70062	MS.gene70061	PPI
MS.gene055423	MS.gene70061	PPI
MS.gene032599	MS.gene70061	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70061.t1	MTR_5g046410	97.748	222	4	1	1	222	1	221	9.95e-158	435
MS.gene70061.t1	MTR_5g046430	97.748	222	4	1	1	222	1	221	9.95e-158	435
MS.gene70061.t1	MTR_4g017030	97.748	222	4	1	1	222	1	221	9.95e-158	435
MS.gene70061.t1	MTR_4g017050	97.297	222	5	1	1	222	1	221	3.48e-157	434
MS.gene70061.t1	MTR_2g019250	95.495	222	9	1	1	222	1	221	1.30e-155	430
MS.gene70061.t1	MTR_4g017040	95.946	222	8	1	1	222	1	221	1.67e-155	430
MS.gene70061.t1	MTR_1g079490	79.821	223	43	2	1	222	1	222	4.64e-127	358
MS.gene70061.t1	MTR_6g005360	74.775	222	54	1	1	222	1	220	5.57e-118	335
MS.gene70061.t1	MTR_6g005340	76.682	223	48	3	1	222	1	220	5.69e-118	335
MS.gene70061.t1	MTR_6g005330	76.233	223	49	3	1	222	1	220	8.64e-118	334
MS.gene70061.t1	MTR_6g005310	76.577	222	48	3	1	221	1	219	2.05e-117	333
MS.gene70061.t1	MTR_6g005350	63.063	222	79	2	1	222	1	219	9.81e-92	268
MS.gene70061.t1	MTR_6g005380	60.766	209	81	1	1	209	1	208	1.56e-87	257
MS.gene70061.t1	MTR_1g023770	59.361	219	75	4	1	216	1	208	6.97e-81	241
MS.gene70061.t1	MTR_2g030855	60.697	201	68	4	19	216	19	211	2.55e-77	231
MS.gene70061.t1	MTR_2g031270	57.727	220	81	5	1	216	1	212	2.42e-73	221
MS.gene70061.t1	MTR_2g030895	64.368	174	55	4	47	216	7	177	7.68e-71	214
MS.gene70061.t1	MTR_2g030865	63.793	174	56	4	47	216	7	177	7.70e-69	209
MS.gene70061.t1	MTR_1g085500	46.296	216	108	3	3	216	4	213	6.41e-61	190
MS.gene70061.t1	MTR_1g085140	46.766	201	99	3	18	216	20	214	1.82e-54	174
MS.gene70061.t1	MTR_7g111240	47.208	197	96	3	22	216	20	210	1.97e-52	168
MS.gene70061.t1	MTR_2g031300	54.651	172	52	4	47	215	7	155	3.19e-51	163
MS.gene70061.t1	MTR_7g111240	47.208	197	93	3	22	216	20	207	3.88e-51	165
MS.gene70061.t1	MTR_5g036570	45.455	198	99	4	22	216	27	218	1.26e-48	159
MS.gene70061.t1	MTR_1g112200	42.000	200	101	5	19	216	20	206	2.08e-44	147
MS.gene70061.t1	MTR_1g060120	36.036	222	129	5	2	216	5	220	2.18e-41	140
MS.gene70061.t1	MTR_2g041550	41.315	213	107	5	4	214	7	203	7.25e-41	139
MS.gene70061.t1	MTR_2g041570	38.605	215	114	5	3	215	6	204	2.99e-39	134
MS.gene70061.t1	MTR_2g041580	38.785	214	113	5	3	214	6	203	3.44e-39	134
MS.gene70061.t1	MTR_8g007300	40.299	201	106	4	22	220	26	214	1.06e-36	128
MS.gene70061.t1	MTR_8g020750	40.404	198	97	5	1	191	1	184	1.03e-34	123
MS.gene70061.t1	MTR_3g019710	39.691	194	106	5	7	198	3	187	2.22e-33	119
MS.gene70061.t1	MTR_8g020590	37.788	217	118	6	1	215	1	202	2.57e-33	119
MS.gene70061.t1	MTR_8g020630	39.205	176	97	4	7	180	3	170	5.77e-33	118
MS.gene70061.t1	MTR_2g036120	37.264	212	116	6	1	207	1	200	4.21e-31	113
MS.gene70061.t1	MTR_8g020770	41.667	180	92	4	1	177	1	170	6.54e-31	113
MS.gene70061.t1	MTR_8g020650	36.269	193	111	5	26	216	22	204	7.11e-31	113
MS.gene70061.t1	MTR_8g020670	34.419	215	123	6	4	216	6	204	9.43e-30	110
MS.gene70061.t1	MTR_3g019650	40.000	175	94	5	26	198	21	186	9.91e-30	110
MS.gene70061.t1	MTR_3g019680	37.563	197	105	6	4	198	6	186	1.31e-29	109
MS.gene70061.t1	MTR_0284s0010	36.893	206	117	5	7	207	3	200	2.62e-29	108
MS.gene70061.t1	MTR_2g044040	36.782	174	100	4	7	178	3	168	1.14e-28	107
MS.gene70061.t1	MTR_2g086640	33.945	218	117	7	2	215	7	201	3.00e-28	106
MS.gene70061.t1	MTR_4g052780	36.585	205	103	6	16	215	21	203	3.06e-28	106
MS.gene70061.t1	MTR_2g045100	41.290	155	81	4	26	178	22	168	3.57e-28	106
MS.gene70061.t1	MTR_2g086620	42.424	132	69	3	85	215	77	202	4.42e-28	105
MS.gene70061.t1	MTR_4g052770	36.098	205	104	6	16	215	21	203	4.48e-28	105
MS.gene70061.t1	MTR_3g096330	38.333	180	104	3	3	180	558	732	5.66e-28	111
MS.gene70061.t1	MTR_2g086630	41.667	132	70	3	85	215	77	202	1.46e-27	104
MS.gene70061.t1	MTR_5g031460	34.597	211	123	5	1	207	1	200	9.03e-27	102
MS.gene70061.t1	MTR_4g010340	35.000	220	118	7	1	216	2	200	1.78e-25	99.0
MS.gene70061.t1	MTR_8g020760	33.945	218	130	6	1	216	1	206	1.78e-25	99.4
MS.gene70061.t1	MTR_7g011340	37.433	187	104	5	26	207	22	200	2.52e-25	98.6
MS.gene70061.t1	MTR_2g019780	32.727	220	131	7	1	216	1	207	2.14e-24	96.3
MS.gene70061.t1	MTR_8g020763	35.227	176	105	3	43	216	17	185	4.36e-24	95.1
MS.gene70061.t1	MTR_3g073170	34.896	192	109	6	28	216	27	205	4.82e-24	95.5
MS.gene70061.t1	MTR_8g020610	33.333	216	126	7	1	214	1	200	7.48e-24	94.7
MS.gene70061.t1	MTR_2g030915	49.351	77	23	1	93	169	1	61	3.61e-18	77.0
MS.gene70061.t1	MTR_3g019810	33.495	206	114	9	1	203	107	292	1.21e-17	80.1
MS.gene70061.t1	MTR_4g027415	37.984	129	70	4	26	152	22	142	1.93e-16	73.6
MS.gene70061.t1	MTR_2g072560	36.066	122	69	4	1	120	1	115	1.37e-12	63.5
MS.gene70061.t1	MTR_3g019610	37.736	106	61	3	7	110	3	105	7.01e-11	57.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70061.t1	AT3G05950	65.044	226	70	5	4	222	5	228	3.17e-95	278
MS.gene70061.t1	AT5G39150	64.151	212	72	3	7	216	9	218	2.23e-92	270
MS.gene70061.t1	AT5G39120	64.151	212	72	3	7	216	9	218	4.20e-92	270
MS.gene70061.t1	AT5G39180	64.151	212	72	3	7	216	9	218	4.79e-92	269
MS.gene70061.t1	AT5G39160	65.238	210	69	3	9	216	11	218	1.62e-90	265
MS.gene70061.t1	AT5G39110	63.679	212	74	2	7	216	9	219	1.66e-90	265
MS.gene70061.t1	AT5G39190	65.238	210	69	3	9	216	11	218	2.43e-90	265
MS.gene70061.t1	AT5G39130	64.286	210	71	3	9	216	11	218	7.66e-90	264
MS.gene70061.t1	AT4G14630	60.000	220	83	3	1	216	3	221	3.83e-86	254
MS.gene70061.t1	AT5G39160	62.857	210	70	3	9	216	11	214	1.37e-85	253
MS.gene70061.t1	AT3G04200	61.722	209	76	4	8	214	13	219	3.37e-82	244
MS.gene70061.t1	AT5G38910	59.729	221	81	4	1	216	1	218	7.42e-80	238
MS.gene70061.t1	AT5G39190	59.135	208	63	2	9	216	11	196	3.31e-78	233
MS.gene70061.t1	AT5G38940	60.914	197	73	2	22	216	26	220	4.86e-78	234
MS.gene70061.t1	AT5G38930	61.809	199	72	2	20	216	24	220	5.72e-78	234
MS.gene70061.t1	AT5G38940	60.914	197	73	2	22	216	50	244	6.69e-78	234
MS.gene70061.t1	AT5G39160	58.654	208	64	2	9	216	11	196	7.76e-78	233
MS.gene70061.t1	AT5G38960	59.901	202	76	3	18	216	22	221	9.67e-78	233
MS.gene70061.t1	AT3G04170	55.238	210	87	4	8	215	12	216	1.16e-72	220
MS.gene70061.t1	AT3G04190	55.446	202	86	3	15	214	19	218	4.31e-72	219
MS.gene70061.t1	AT3G04150	53.917	217	93	4	4	215	5	219	5.89e-72	219
MS.gene70061.t1	AT3G04150	51.092	229	94	4	4	215	5	232	5.66e-70	214
MS.gene70061.t1	AT3G04180	53.960	202	89	3	15	214	19	218	4.39e-69	211
MS.gene70061.t1	AT1G02335	43.721	215	113	3	4	216	6	214	1.36e-56	179
MS.gene70061.t1	AT5G38910	48.416	221	69	4	1	216	1	181	7.98e-56	176
MS.gene70061.t1	AT3G62020	46.262	214	105	5	6	216	8	214	2.47e-55	176
MS.gene70061.t1	AT1G09560	45.545	202	102	3	17	216	19	214	8.01e-54	172
MS.gene70061.t1	AT3G05930	47.783	203	96	4	4	203	6	201	2.88e-53	171
MS.gene70061.t1	AT5G26700	50.000	184	77	5	26	205	25	197	2.45e-51	166
MS.gene70061.t1	AT1G18970	44.776	201	101	4	18	216	25	217	2.00e-49	161
MS.gene70061.t1	AT1G18980	45.274	201	100	4	18	216	25	217	3.39e-48	158
MS.gene70061.t1	AT3G62020	46.961	181	89	3	39	216	9	185	8.42e-48	156
MS.gene70061.t1	AT1G10460	41.709	199	107	5	22	215	19	213	4.28e-45	150
MS.gene70061.t1	AT3G10080	39.409	203	113	5	18	216	22	218	1.60e-43	146
MS.gene70061.t1	AT3G10080	39.409	203	113	5	18	216	93	289	7.13e-43	146
MS.gene70061.t1	AT5G61750	41.398	186	103	2	4	187	1	182	1.47e-40	138
MS.gene70061.t1	AT5G20630	41.232	211	111	6	1	209	1	200	1.68e-40	138
MS.gene70061.t1	AT1G72610	39.791	191	105	6	26	214	19	201	1.68e-32	117
MS.gene70061.t1	AT1G74820	38.660	194	109	4	25	216	39	224	2.75e-32	117
MS.gene70061.t1	AT5G38950	54.167	72	33	0	146	217	2	73	1.26e-18	78.2
MS.gene70061.t1	AT5G39100	72.000	50	14	0	7	56	9	58	7.61e-18	74.7

Find 54 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATATACATACCACCATCTT+TGG	0.255130	4.2:-84934880	None:intergenic
TTTCAACTGAATGTTGGATA+TGG	0.274546	4.2:+84935492	MS.gene70061:CDS
TAACACTCTTGGTATATCTT+TGG	0.286436	4.2:+84935278	MS.gene70061:CDS
CCTTGTTAGCATCTTGGCTT+TGG	0.311083	4.2:+84934791	MS.gene70061:CDS
TTGAGAGCCTAGTGCGTTAG+AGG	0.314204	4.2:-84935220	None:intergenic
CTTATCCACTTGAAAAGCTT+TGG	0.335788	4.2:-84935619	None:intergenic
TGAAAATGAATAAGACCAAT+TGG	0.356438	4.2:-84935474	None:intergenic
GAGTGCCTTCAAGTACTATA+AGG	0.386700	4.2:-84935365	None:intergenic
ATACTTCCTTGTTAGCATCT+TGG	0.401215	4.2:+84934785	MS.gene70061:CDS
ATATACATACCACCATCTTT+GGG	0.405165	4.2:-84934879	None:intergenic
TTCTTCAAACATATTGAAGC+TGG	0.411728	4.2:+84935192	MS.gene70061:CDS
TTGAAGAGGGCTAGGGTCAT+AGG	0.420416	4.2:-84934832	None:intergenic
GATGTGTTTGTGTTCCCAAT+TGG	0.424230	4.2:+84935459	MS.gene70061:CDS
GGCAACGCGGTTGCTATCGC+TGG	0.429748	4.2:+84935513	MS.gene70061:CDS
CAAAAGTCTTGAAGAGGGCT+AGG	0.441824	4.2:-84934840	None:intergenic
ATTATCTTCAAAAGCAGTCT+TGG	0.442184	4.2:+84935652	MS.gene70061:CDS
TCTTCAGCTTTAACAAGTGC+GGG	0.444792	4.2:-84935168	None:intergenic
GTCACTGTAGACCAACTATT+CGG	0.446134	4.2:+84935252	MS.gene70061:CDS
CTTGAAGGCACTCTTTATGT+TGG	0.454430	4.2:+84935375	MS.gene70061:CDS
TACTTTGCAGTATTTGTGAA+TGG	0.463825	4.2:+84935129	MS.gene70061:intron
ATAAGGATCTCTGTTCCTCT+AGG	0.468428	4.2:-84935348	None:intergenic
GAGATCCTTATAGTACTTGA+AGG	0.469573	4.2:+84935360	MS.gene70061:CDS
TTTGCAACGGTGATAACTCC+TGG	0.470922	4.2:-84935552	None:intergenic
CTATTCGGACTTAACACTCT+TGG	0.480493	4.2:+84935267	MS.gene70061:CDS
CTCTAGGGTGAATGTGAGGT+GGG	0.498933	4.2:-84935332	None:intergenic
GAAAATGAATAAGACCAATT+GGG	0.510497	4.2:-84935473	None:intergenic
AGAACTTCGTCGGAAATAAG+AGG	0.511108	4.2:-84935594	None:intergenic
TCTTCAAACATATTGAAGCT+GGG	0.520485	4.2:+84935193	MS.gene70061:CDS
GAGTGTTAAGTCCGAATAGT+TGG	0.535207	4.2:-84935263	None:intergenic
TGAGGTGGGTTTAAACCCTT+AGG	0.539104	4.2:-84935318	None:intergenic
ATCTTCAGCTTTAACAAGTG+CGG	0.543612	4.2:-84935169	None:intergenic
CTTTACCAAGGTGCTCAACA+AGG	0.544491	4.2:+84935434	MS.gene70061:CDS
GCAATCAAAGACCCCAAAGA+TGG	0.554146	4.2:+84934867	MS.gene70061:CDS
AGCTTTGGTAAGAACTTCGT+CGG	0.555884	4.2:-84935604	None:intergenic
CCAAAGCCAAGATGCTAACA+AGG	0.556137	4.2:-84934791	None:intergenic
ATCACCCTTGTTGAGCACCT+TGG	0.567836	4.2:-84935439	None:intergenic
GGGAATGCCTCTAACGCACT+AGG	0.573570	4.2:+84935213	MS.gene70061:CDS
GGACTTAGCAGTCAAAATCC+AGG	0.578792	4.2:+84935534	MS.gene70061:CDS
TAAGGATCTCTGTTCCTCTA+GGG	0.579174	4.2:-84935347	None:intergenic
CCTCTAGGGTGAATGTGAGG+TGG	0.583739	4.2:-84935333	None:intergenic
TTTAAACAAAGCATTTGCAA+CGG	0.590900	4.2:-84935565	None:intergenic
AGGGCTAGGGTCATAGGCAA+AGG	0.601750	4.2:-84934826	None:intergenic
GCAACACAAAAGTCTTGAAG+AGG	0.609121	4.2:-84934846	None:intergenic
AAAAGTCTTGAAGAGGGCTA+GGG	0.618701	4.2:-84934839	None:intergenic
ATCAAAGACCCCAAAGATGG+TGG	0.628410	4.2:+84934870	MS.gene70061:CDS
GTCATAGGCAAAGGCAACAG+AGG	0.637895	4.2:-84934817	None:intergenic
TATACATACCACCATCTTTG+GGG	0.639620	4.2:-84934878	None:intergenic
GTTCCTCTAGGGTGAATGTG+AGG	0.650118	4.2:-84935336	None:intergenic
CAACACAAAAGTCTTGAAGA+GGG	0.657826	4.2:-84934845	None:intergenic
TTTACCAAGGTGCTCAACAA+GGG	0.659466	4.2:+84935435	MS.gene70061:CDS
CTTCAGCTTTAACAAGTGCG+GGG	0.662073	4.2:-84935167	None:intergenic
CAATAATCGTCTCTTTACCA+AGG	0.672342	4.2:+84935422	MS.gene70061:CDS
CCACCTCACATTCACCCTAG+AGG	0.685266	4.2:+84935333	MS.gene70061:CDS
GAATGTTGGATATGGCAACG+CGG	0.840401	4.2:+84935500	MS.gene70061:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAAAAATATATATTTTTGCA+GGG	-	chr4.2:84934995-84935014	None:intergenic	10.0%
!!!	AAATATAGAATCTTTTGTTT+TGG	+	chr4.2:84935030-84935049	MS.gene70061:intron	15.0%
!!!	CAAAAAATATATATTTTTGC+AGG	-	chr4.2:84934996-84935015	None:intergenic	15.0%
!!!	CTGCAAAAATATATATTTTT+TGG	+	chr4.2:84934994-84935013	MS.gene70061:intron	15.0%
!!	GTAATCAAGACTTAAAATTA+AGG	-	chr4.2:84934908-84934927	None:intergenic	20.0%
!	GAAAATGAATAAGACCAATT+GGG	-	chr4.2:84935476-84935495	None:intergenic	25.0%
!	TGAAAATGAATAAGACCAAT+TGG	-	chr4.2:84935477-84935496	None:intergenic	25.0%
!!	ATTCATTTTCAACTGAATGT+TGG	+	chr4.2:84935486-84935505	MS.gene70061:CDS	25.0%
!!	TTTAAACAAAGCATTTGCAA+CGG	-	chr4.2:84935568-84935587	None:intergenic	25.0%
	ATATACATACCACCATCTTT+GGG	-	chr4.2:84934882-84934901	None:intergenic	30.0%
	ATTATCTTCAAAAGCAGTCT+TGG	+	chr4.2:84935652-84935671	MS.gene70061:CDS	30.0%
	GCAAAAAGTCTAATATTTGC+AGG	-	chr4.2:84934965-84934984	None:intergenic	30.0%
	TAACACTCTTGGTATATCTT+TGG	+	chr4.2:84935278-84935297	MS.gene70061:CDS	30.0%
	TACTTTGCAGTATTTGTGAA+TGG	+	chr4.2:84935129-84935148	MS.gene70061:intron	30.0%
	TATATACATACCACCATCTT+TGG	-	chr4.2:84934883-84934902	None:intergenic	30.0%
	TCTTCAAACATATTGAAGCT+GGG	+	chr4.2:84935193-84935212	MS.gene70061:CDS	30.0%
	TTCTTCAAACATATTGAAGC+TGG	+	chr4.2:84935192-84935211	MS.gene70061:CDS	30.0%
	TTTCAACTGAATGTTGGATA+TGG	+	chr4.2:84935492-84935511	MS.gene70061:CDS	30.0%
!!!	GAATCTTTTGTTTTGGTTCT+TGG	+	chr4.2:84935037-84935056	MS.gene70061:intron	30.0%
	ATACTTCCTTGTTAGCATCT+TGG	+	chr4.2:84934785-84934804	MS.gene70061:CDS	35.0%
	CAACACAAAAGTCTTGAAGA+GGG	-	chr4.2:84934848-84934867	None:intergenic	35.0%
	CAATAATCGTCTCTTTACCA+AGG	+	chr4.2:84935422-84935441	MS.gene70061:CDS	35.0%
	CTTATCCACTTGAAAAGCTT+TGG	-	chr4.2:84935622-84935641	None:intergenic	35.0%
	GAGATCCTTATAGTACTTGA+AGG	+	chr4.2:84935360-84935379	MS.gene70061:CDS	35.0%
	TATACATACCACCATCTTTG+GGG	-	chr4.2:84934881-84934900	None:intergenic	35.0%
!	ATCTTCAGCTTTAACAAGTG+CGG	-	chr4.2:84935172-84935191	None:intergenic	35.0%
!!	TCTTACCAAAGCTTTTCAAG+TGG	+	chr4.2:84935614-84935633	MS.gene70061:CDS	35.0%
	AAAAGTCTTGAAGAGGGCTA+GGG	-	chr4.2:84934842-84934861	None:intergenic	40.0%
	AGAACTTCGTCGGAAATAAG+AGG	-	chr4.2:84935597-84935616	None:intergenic	40.0%
	AGTAATGACCTACCTAAACC+AGG	-	chr4.2:84935068-84935087	None:intergenic	40.0%
	ATAAGGATCTCTGTTCCTCT+AGG	-	chr4.2:84935351-84935370	None:intergenic	40.0%
	CTATTCGGACTTAACACTCT+TGG	+	chr4.2:84935267-84935286	MS.gene70061:CDS	40.0%
	GAGTGCCTTCAAGTACTATA+AGG	-	chr4.2:84935368-84935387	None:intergenic	40.0%
	GAGTGTTAAGTCCGAATAGT+TGG	-	chr4.2:84935266-84935285	None:intergenic	40.0%
	GATGTGTTTGTGTTCCCAAT+TGG	+	chr4.2:84935459-84935478	MS.gene70061:CDS	40.0%
	GCAACACAAAAGTCTTGAAG+AGG	-	chr4.2:84934849-84934868	None:intergenic	40.0%
	GTCACTGTAGACCAACTATT+CGG	+	chr4.2:84935252-84935271	MS.gene70061:CDS	40.0%
	TAAGGATCTCTGTTCCTCTA+GGG	-	chr4.2:84935350-84935369	None:intergenic	40.0%
!	AGCTTTGGTAAGAACTTCGT+CGG	-	chr4.2:84935607-84935626	None:intergenic	40.0%
!	TCTTCAGCTTTAACAAGTGC+GGG	-	chr4.2:84935171-84935190	None:intergenic	40.0%
!	TTTACCAAGGTGCTCAACAA+GGG	+	chr4.2:84935435-84935454	MS.gene70061:CDS	40.0%
!!	CTTGAAGGCACTCTTTATGT+TGG	+	chr4.2:84935375-84935394	MS.gene70061:CDS	40.0%
!!	TTCTTGGTGTTTCCTGGTTT+AGG	+	chr4.2:84935053-84935072	MS.gene70061:intron	40.0%
!!!	TTTTGGTTCTTGGTGTTTCC+TGG	+	chr4.2:84935047-84935066	MS.gene70061:intron	40.0%
	ATCAAAGACCCCAAAGATGG+TGG	+	chr4.2:84934870-84934889	MS.gene70061:CDS	45.0%
	CAAAAGTCTTGAAGAGGGCT+AGG	-	chr4.2:84934843-84934862	None:intergenic	45.0%
	CCAAAGCCAAGATGCTAACA+AGG	-	chr4.2:84934794-84934813	None:intergenic	45.0%
	CCTTGTTAGCATCTTGGCTT+TGG	+	chr4.2:84934791-84934810	MS.gene70061:CDS	45.0%
	GAATGTTGGATATGGCAACG+CGG	+	chr4.2:84935500-84935519	MS.gene70061:CDS	45.0%
	GCAATCAAAGACCCCAAAGA+TGG	+	chr4.2:84934867-84934886	MS.gene70061:CDS	45.0%
	GGACTTAGCAGTCAAAATCC+AGG	+	chr4.2:84935534-84935553	MS.gene70061:CDS	45.0%
	TGGTGTTTCCTGGTTTAGGT+AGG	+	chr4.2:84935057-84935076	MS.gene70061:intron	45.0%
	TTTGCAACGGTGATAACTCC+TGG	-	chr4.2:84935555-84935574	None:intergenic	45.0%
!	CTTCAGCTTTAACAAGTGCG+GGG	-	chr4.2:84935170-84935189	None:intergenic	45.0%
!	CTTTACCAAGGTGCTCAACA+AGG	+	chr4.2:84935434-84935453	MS.gene70061:CDS	45.0%
!	TGAGGTGGGTTTAAACCCTT+AGG	-	chr4.2:84935321-84935340	None:intergenic	45.0%
!!!	CGTGTTGATTTTGCGCCTAA+GGG	+	chr4.2:84935303-84935322	MS.gene70061:CDS	45.0%
!!!	TCGTGTTGATTTTGCGCCTA+AGG	+	chr4.2:84935302-84935321	MS.gene70061:CDS	45.0%
	ATCACCCTTGTTGAGCACCT+TGG	-	chr4.2:84935442-84935461	None:intergenic	50.0%
	GTCATAGGCAAAGGCAACAG+AGG	-	chr4.2:84934820-84934839	None:intergenic	50.0%
	TTGAAGAGGGCTAGGGTCAT+AGG	-	chr4.2:84934835-84934854	None:intergenic	50.0%
	TTGAGAGCCTAGTGCGTTAG+AGG	-	chr4.2:84935223-84935242	None:intergenic	50.0%
!!	CTCTAGGGTGAATGTGAGGT+GGG	-	chr4.2:84935335-84935354	None:intergenic	50.0%
!!	GTTCCTCTAGGGTGAATGTG+AGG	-	chr4.2:84935339-84935358	None:intergenic	50.0%
	AGGGCTAGGGTCATAGGCAA+AGG	-	chr4.2:84934829-84934848	None:intergenic	55.0%
	CCACCTCACATTCACCCTAG+AGG	+	chr4.2:84935333-84935352	MS.gene70061:CDS	55.0%
	GGGAATGCCTCTAACGCACT+AGG	+	chr4.2:84935213-84935232	MS.gene70061:CDS	55.0%
!!	CCTCTAGGGTGAATGTGAGG+TGG	-	chr4.2:84935336-84935355	None:intergenic	55.0%
	GGCAACGCGGTTGCTATCGC+TGG	+	chr4.2:84935513-84935532	MS.gene70061:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	84934774	84935689	84934774	ID=MS.gene70061
chr4.2	mRNA	84934774	84935689	84934774	ID=MS.gene70061.t1;Parent=MS.gene70061
chr4.2	exon	84934774	84934891	84934774	ID=MS.gene70061.t1.exon1;Parent=MS.gene70061.t1
chr4.2	CDS	84934774	84934891	84934774	ID=cds.MS.gene70061.t1;Parent=MS.gene70061.t1
chr4.2	exon	84935139	84935689	84935139	ID=MS.gene70061.t1.exon2;Parent=MS.gene70061.t1
chr4.2	CDS	84935139	84935689	84935139	ID=cds.MS.gene70061.t1;Parent=MS.gene70061.t1

Gene Sequence

>MS.gene70061

ATGAAGACTATATACTTCCTTGTTAGCATCTTGGCTTTGGCATCCTCTGTTGCCTTTGCCTATGACCCTAGCCCTCTTCAAGACTTTTGTGTTGCAATCAAAGACCCCAAAGATGGTGGTATGTATATATTCCTTAATTTTAAGTCTTGATTACTACTCGATTTTTATGCTTAAATATGCAAATGTTTCCTGCAAATATTAGACTTTTTGCTATAAATCCCTGCAAAAATATATATTTTTTGGTTTTAGCGCTTGTAAATATAGAATCTTTTGTTTTGGTTCTTGGTGTTTCCTGGTTTAGGTAGGTCATTACTAGACACTATAATTTCTTTGATATAACTAAACTATGATTTGATACTTTGCAGTATTTGTGAATGGAAAATTCTGTAAAGACCCCGCACTTGTTAAAGCTGAAGATTTCTTCAAACATATTGAAGCTGGGAATGCCTCTAACGCACTAGGCTCTCAAGTAACTCAAGTCACTGTAGACCAACTATTCGGACTTAACACTCTTGGTATATCTTTGGCTCGTGTTGATTTTGCGCCTAAGGGTTTAAACCCACCTCACATTCACCCTAGAGGAACAGAGATCCTTATAGTACTTGAAGGCACTCTTTATGTTGGATTTGTCACGTCAAATCAAGACAACAATAATCGTCTCTTTACCAAGGTGCTCAACAAGGGTGATGTGTTTGTGTTCCCAATTGGTCTTATTCATTTTCAACTGAATGTTGGATATGGCAACGCGGTTGCTATCGCTGGACTTAGCAGTCAAAATCCAGGAGTTATCACCGTTGCAAATGCTTTGTTTAAATCTGATCCTCTTATTTCCGACGAAGTTCTTACCAAAGCTTTTCAAGTGGATAAGAGCATAATTGATTATCTTCAAAAGCAGTCTTGGTATGACAACAACTAG

Protein sequence

>MS.gene70061.t1

MKTIYFLVSILALASSVAFAYDPSPLQDFCVAIKDPKDGVFVNGKFCKDPALVKAEDFFKHIEAGNASNALGSQVTQVTVDQLFGLNTLGISLARVDFAPKGLNPPHIHPRGTEILIVLEGTLYVGFVTSNQDNNNRLFTKVLNKGDVFVFPIGLIHFQLNVGYGNAVAIAGLSSQNPGVITVANALFKSDPLISDEVLTKAFQVDKSIIDYLQKQSWYDNN