AlfalfaGEDB | MS.gene70062

M. sativa cultivar XinJiangDaYe / MS.gene70062

KEGG: -
GO: GO:0003674 GO:0005488 GO:0030145 GO:0043167 GO:0043169 GO:0045735 GO:0046872 GO:0046914
PFAM: PF00190.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70062.t1	XP_003604729.1	97.3	221	6	0	1	221	1	221	1.90E-117	431.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70062.t1	Q9SFF9	65.9	223	68	4	6	221	7	228	5.0e-74	278.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70062.t1	G7JQ50	97.3	221	6	0	1	221	1	221	1.4e-117	431.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04932	MS.gene70062	0.800889	1.17E-48	-1.69E-46
MS.gene049549	MS.gene70062	0.807144	5.83E-50	-1.69E-46
MS.gene050096	MS.gene70062	0.828887	7.00E-55	-1.69E-46
MS.gene050380	MS.gene70062	0.815799	7.68E-52	-1.69E-46
MS.gene050529	MS.gene70062	-0.800604	1.33E-48	-1.69E-46
MS.gene050670	MS.gene70062	-0.808689	2.74E-50	-1.69E-46
MS.gene050809	MS.gene70062	0.81605	6.75E-52	-1.69E-46
MS.gene050971	MS.gene70062	-0.818155	2.27E-52	-1.69E-46
MS.gene051158	MS.gene70062	0.82873	7.64E-55	-1.69E-46
MS.gene051385	MS.gene70062	0.808494	3.01E-50	-1.69E-46
MS.gene051632	MS.gene70062	0.818658	1.75E-52	-1.69E-46
MS.gene051996	MS.gene70062	0.827928	1.19E-54	-1.69E-46
MS.gene052188	MS.gene70062	-0.805681	1.19E-49	-1.69E-46
MS.gene052223	MS.gene70062	0.832457	9.35E-56	-1.69E-46
MS.gene05240	MS.gene70062	0.844557	7.02E-59	-1.69E-46
MS.gene05244	MS.gene70062	0.825138	5.52E-54	-1.69E-46
MS.gene052702	MS.gene70062	0.816874	4.42E-52	-1.69E-46
MS.gene052706	MS.gene70062	0.81792	2.57E-52	-1.69E-46
MS.gene052805	MS.gene70062	0.802354	5.84E-49	-1.69E-46
MS.gene053031	MS.gene70062	0.838402	2.94E-57	-1.69E-46
MS.gene053033	MS.gene70062	0.802807	4.71E-49	-1.69E-46
MS.gene053406	MS.gene70062	0.82817	1.04E-54	-1.69E-46
MS.gene053912	MS.gene70062	-0.802511	5.42E-49	-1.69E-46
MS.gene054181	MS.gene70062	0.826191	3.11E-54	-1.69E-46
MS.gene054277	MS.gene70062	0.815036	1.14E-51	-1.69E-46
MS.gene054290	MS.gene70062	0.868162	8.02E-66	-1.69E-46
MS.gene054742	MS.gene70062	0.848425	6.18E-60	-1.69E-46
MS.gene055601	MS.gene70062	0.834045	3.76E-56	-1.69E-46
MS.gene055959	MS.gene70062	0.849352	3.42E-60	-1.69E-46
MS.gene056330	MS.gene70062	0.836589	8.57E-57	-1.69E-46
MS.gene056511	MS.gene70062	-0.80218	6.34E-49	-1.69E-46
MS.gene056590	MS.gene70062	0.810304	1.23E-50	-1.69E-46
MS.gene056591	MS.gene70062	0.808342	3.25E-50	-1.69E-46
MS.gene056690	MS.gene70062	0.810335	1.21E-50	-1.69E-46
MS.gene056691	MS.gene70062	0.804564	2.03E-49	-1.69E-46
MS.gene056899	MS.gene70062	0.829242	5.74E-55	-1.69E-46
MS.gene056920	MS.gene70062	0.833877	4.14E-56	-1.69E-46
MS.gene056923	MS.gene70062	0.83124	1.87E-55	-1.69E-46
MS.gene056924	MS.gene70062	0.814855	1.25E-51	-1.69E-46
MS.gene057537	MS.gene70062	-0.802036	6.79E-49	-1.69E-46
MS.gene057544	MS.gene70062	0.864831	9.16E-65	-1.69E-46
MS.gene057749	MS.gene70062	-0.8136	2.36E-51	-1.69E-46
MS.gene057823	MS.gene70062	-0.807341	5.30E-50	-1.69E-46
MS.gene058093	MS.gene70062	0.823011	1.74E-53	-1.69E-46
MS.gene058177	MS.gene70062	0.830805	2.39E-55	-1.69E-46
MS.gene058179	MS.gene70062	0.820902	5.37E-53	-1.69E-46
MS.gene058218	MS.gene70062	0.851908	6.53E-61	-1.69E-46
MS.gene058660	MS.gene70062	-0.800031	1.74E-48	-1.69E-46
MS.gene058928	MS.gene70062	-0.81292	3.33E-51	-1.69E-46
MS.gene059037	MS.gene70062	0.804658	1.94E-49	-1.69E-46
MS.gene059043	MS.gene70062	0.837246	5.82E-57	-1.69E-46
MS.gene059294	MS.gene70062	-0.828565	8.37E-55	-1.69E-46
MS.gene059402	MS.gene70062	0.839788	1.28E-57	-1.69E-46
MS.gene059944	MS.gene70062	0.805563	1.26E-49	-1.69E-46
MS.gene059955	MS.gene70062	0.815167	1.06E-51	-1.69E-46
MS.gene060087	MS.gene70062	0.818573	1.83E-52	-1.69E-46
MS.gene060804	MS.gene70062	-0.804537	2.06E-49	-1.69E-46
MS.gene061041	MS.gene70062	-0.802452	5.57E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene70062	MS.gene82857	PPI
MS.gene002728	MS.gene70062	PPI
MS.gene037517	MS.gene70062	PPI
MS.gene032596	MS.gene70062	PPI
MS.gene022634	MS.gene70062	PPI
MS.gene89772	MS.gene70062	PPI
MS.gene70062	MS.gene037519	PPI
MS.gene037516	MS.gene70062	PPI
MS.gene70062	MS.gene005041	PPI
MS.gene50790	MS.gene70062	PPI
MS.gene70062	MS.gene82859	PPI
MS.gene032598	MS.gene70062	PPI
MS.gene89773	MS.gene70062	PPI
MS.gene70063	MS.gene70062	PPI
MS.gene053033	MS.gene70062	PPI
MS.gene053027	MS.gene70062	PPI
MS.gene70064	MS.gene70062	PPI
MS.gene74887	MS.gene70062	PPI
MS.gene70062	MS.gene82855	PPI
MS.gene50787	MS.gene70062	PPI
MS.gene70062	MS.gene96244	PPI
MS.gene032599	MS.gene70062	PPI
MS.gene89774	MS.gene70062	PPI
MS.gene70062	MS.gene053031	PPI
MS.gene053029	MS.gene70062	PPI
MS.gene053030	MS.gene70062	PPI
MS.gene70062	MS.gene70063	PPI
MS.gene70062	MS.gene07591	PPI
MS.gene70062	MS.gene037520	PPI
MS.gene89775	MS.gene70062	PPI
MS.gene50786	MS.gene70062	PPI
MS.gene032601	MS.gene70062	PPI
MS.gene053026	MS.gene70062	PPI
MS.gene99166	MS.gene70062	PPI
MS.gene70062	MS.gene032596	PPI
MS.gene70062	MS.gene50789	PPI
MS.gene96244	MS.gene70062	PPI
MS.gene70062	MS.gene037518	PPI
MS.gene70062	MS.gene50788	PPI
MS.gene81739	MS.gene70062	PPI
MS.gene056870	MS.gene70062	PPI
MS.gene70062	MS.gene50787	PPI
MS.gene70062	MS.gene055423	PPI
MS.gene034877	MS.gene70062	PPI
MS.gene70062	MS.gene047464	PPI
MS.gene70062	MS.gene89774	PPI
MS.gene82858	MS.gene70062	PPI
MS.gene70062	MS.gene82860	PPI
MS.gene70062	MS.gene037851	PPI
MS.gene70062	MS.gene032597	PPI
MS.gene70062	MS.gene70061	PPI
MS.gene004411	MS.gene70062	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70062.t1	MTR_5g046410	97.285	221	6	0	1	221	1	221	2.42e-158	437
MS.gene70062.t1	MTR_5g046430	97.285	221	6	0	1	221	1	221	2.42e-158	437
MS.gene70062.t1	MTR_4g017030	97.285	221	6	0	1	221	1	221	2.42e-158	437
MS.gene70062.t1	MTR_4g017050	96.833	221	7	0	1	221	1	221	8.95e-158	436
MS.gene70062.t1	MTR_4g017040	95.475	221	10	0	1	221	1	221	4.96e-156	431
MS.gene70062.t1	MTR_2g019250	95.023	221	11	0	1	221	1	221	6.18e-156	431
MS.gene70062.t1	MTR_1g079490	80.631	222	42	1	1	221	1	222	1.15e-128	362
MS.gene70062.t1	MTR_6g005340	77.027	222	48	3	1	221	1	220	2.33e-117	333
MS.gene70062.t1	MTR_6g005360	75.113	221	54	1	1	221	1	220	2.48e-117	333
MS.gene70062.t1	MTR_6g005330	76.577	222	49	3	1	221	1	220	3.56e-117	333
MS.gene70062.t1	MTR_6g005310	76.923	221	48	3	1	220	1	219	9.35e-117	332
MS.gene70062.t1	MTR_6g005350	62.443	221	81	2	1	221	1	219	1.79e-90	265
MS.gene70062.t1	MTR_6g005380	61.058	208	81	0	1	208	1	208	5.30e-88	258
MS.gene70062.t1	MTR_1g023770	58.986	217	78	3	1	215	1	208	9.04e-80	238
MS.gene70062.t1	MTR_2g030855	60.302	199	69	4	20	215	20	211	4.53e-76	228
MS.gene70062.t1	MTR_2g031270	60.622	193	66	4	26	215	27	212	1.82e-72	219
MS.gene70062.t1	MTR_2g030895	64.162	173	56	4	47	215	7	177	1.47e-70	213
MS.gene70062.t1	MTR_2g030865	63.584	173	57	4	47	215	7	177	1.32e-68	208
MS.gene70062.t1	MTR_1g085500	46.977	215	107	3	3	215	4	213	1.38e-61	192
MS.gene70062.t1	MTR_1g085140	46.000	200	101	3	18	215	20	214	4.06e-53	170
MS.gene70062.t1	MTR_7g111240	47.959	196	95	3	22	215	20	210	1.30e-52	169
MS.gene70062.t1	MTR_7g111240	47.959	196	92	3	22	215	20	207	2.04e-51	166
MS.gene70062.t1	MTR_2g031300	54.386	171	53	4	47	214	7	155	6.25e-51	162
MS.gene70062.t1	MTR_5g036570	45.178	197	100	4	22	215	27	218	5.07e-48	157
MS.gene70062.t1	MTR_1g112200	40.845	213	109	6	8	215	6	206	3.28e-43	144
MS.gene70062.t1	MTR_2g041550	41.981	212	106	5	4	213	7	203	4.99e-42	142
MS.gene70062.t1	MTR_1g060120	36.652	221	128	5	2	215	5	220	2.13e-41	140
MS.gene70062.t1	MTR_2g041570	39.252	214	113	5	3	214	6	204	1.89e-40	137
MS.gene70062.t1	MTR_2g041580	39.437	213	112	5	3	213	6	203	2.00e-40	137
MS.gene70062.t1	MTR_8g007300	38.710	217	119	5	6	219	9	214	1.05e-36	128
MS.gene70062.t1	MTR_8g020750	40.609	197	97	5	1	190	1	184	6.99e-35	123
MS.gene70062.t1	MTR_8g020590	38.426	216	117	6	1	214	1	202	3.47e-34	121
MS.gene70062.t1	MTR_8g020630	38.660	194	102	6	1	190	1	181	1.98e-32	117
MS.gene70062.t1	MTR_3g019710	38.860	193	108	5	7	197	3	187	2.91e-32	116
MS.gene70062.t1	MTR_8g020650	35.938	192	112	5	26	215	22	204	1.33e-30	112
MS.gene70062.t1	MTR_8g020770	40.782	179	94	4	1	176	1	170	2.03e-30	112
MS.gene70062.t1	MTR_2g036120	36.967	211	117	6	1	206	1	200	3.39e-30	111
MS.gene70062.t1	MTR_3g019680	37.245	196	106	6	4	197	6	186	1.08e-29	110
MS.gene70062.t1	MTR_3g019650	37.245	196	106	6	4	197	6	186	1.26e-29	109
MS.gene70062.t1	MTR_8g020670	34.112	214	124	6	4	215	6	204	1.48e-29	109
MS.gene70062.t1	MTR_0284s0010	37.559	213	113	7	1	206	1	200	1.05e-28	107
MS.gene70062.t1	MTR_2g045100	37.129	202	115	5	10	206	6	200	1.48e-28	107
MS.gene70062.t1	MTR_2g044040	36.872	179	100	5	1	177	1	168	4.25e-28	105
MS.gene70062.t1	MTR_2g086640	34.101	217	117	7	2	214	7	201	4.29e-28	105
MS.gene70062.t1	MTR_4g052780	35.784	204	105	6	16	214	21	203	1.01e-27	104
MS.gene70062.t1	MTR_2g086620	42.748	131	69	3	85	214	77	202	1.36e-27	104
MS.gene70062.t1	MTR_3g096330	35.294	204	124	5	5	205	559	757	1.53e-27	110
MS.gene70062.t1	MTR_4g052770	35.294	204	106	6	16	214	21	203	3.05e-27	103
MS.gene70062.t1	MTR_2g086630	41.985	131	70	3	85	214	77	202	3.53e-27	103
MS.gene70062.t1	MTR_5g031460	34.286	210	124	5	1	206	1	200	3.14e-26	100
MS.gene70062.t1	MTR_4g010340	34.703	219	119	7	1	215	2	200	3.14e-25	98.2
MS.gene70062.t1	MTR_7g011340	37.097	186	105	5	26	206	22	200	5.26e-25	97.8
MS.gene70062.t1	MTR_8g020760	35.484	217	127	7	1	215	1	206	7.10e-25	97.4
MS.gene70062.t1	MTR_8g020610	33.953	215	125	7	1	213	1	200	2.16e-24	96.3
MS.gene70062.t1	MTR_8g020763	36.095	169	99	3	43	206	17	181	3.40e-24	95.1
MS.gene70062.t1	MTR_3g073170	35.484	186	104	6	27	206	26	201	7.09e-24	94.7
MS.gene70062.t1	MTR_2g019780	32.420	219	132	7	1	215	1	207	1.82e-23	94.0
MS.gene70062.t1	MTR_2g030915	48.684	76	24	1	93	168	1	61	1.01e-17	75.9
MS.gene70062.t1	MTR_3g019810	32.683	205	116	9	1	202	107	292	1.48e-16	77.0
MS.gene70062.t1	MTR_4g027415	37.500	128	71	4	26	151	22	142	4.89e-16	72.4
MS.gene70062.t1	MTR_2g072560	36.066	122	69	4	1	120	1	115	7.26e-12	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70062.t1	AT3G05950	65.333	225	70	4	4	221	5	228	6.21e-97	282
MS.gene70062.t1	AT5G39150	65.403	211	70	2	7	215	9	218	5.46e-95	277
MS.gene70062.t1	AT5G39120	65.403	211	70	2	7	215	9	218	7.66e-95	276
MS.gene70062.t1	AT5G39180	65.403	211	70	2	7	215	9	218	9.97e-95	276
MS.gene70062.t1	AT5G39110	64.929	211	72	1	7	215	9	219	2.87e-93	272
MS.gene70062.t1	AT5G39160	66.507	209	67	2	9	215	11	218	3.73e-93	272
MS.gene70062.t1	AT5G39190	66.507	209	67	2	9	215	11	218	6.80e-93	271
MS.gene70062.t1	AT5G39130	65.550	209	69	2	9	215	11	218	1.53e-92	271
MS.gene70062.t1	AT4G14630	61.644	219	80	2	1	215	3	221	1.95e-89	263
MS.gene70062.t1	AT5G39160	64.115	209	68	2	9	215	11	214	3.85e-88	259
MS.gene70062.t1	AT3G04200	62.981	208	74	3	8	213	13	219	1.12e-84	251
MS.gene70062.t1	AT5G39190	60.386	207	61	1	9	215	11	196	1.20e-80	239
MS.gene70062.t1	AT5G39160	59.903	207	62	1	9	215	11	196	3.06e-80	238
MS.gene70062.t1	AT5G38910	60.000	220	81	4	1	215	1	218	3.88e-80	239
MS.gene70062.t1	AT5G38960	61.692	201	73	3	18	215	22	221	3.26e-79	237
MS.gene70062.t1	AT5G38930	62.312	199	72	2	19	215	23	220	1.20e-78	235
MS.gene70062.t1	AT5G38940	61.224	196	73	2	22	215	26	220	4.89e-78	234
MS.gene70062.t1	AT5G38940	61.224	196	73	2	22	215	50	244	6.10e-78	234
MS.gene70062.t1	AT3G04190	56.716	201	84	2	15	213	19	218	3.99e-74	224
MS.gene70062.t1	AT3G04170	55.556	207	86	3	10	214	14	216	7.99e-74	223
MS.gene70062.t1	AT3G04150	54.630	216	92	3	4	214	5	219	4.19e-73	221
MS.gene70062.t1	AT3G04150	51.754	228	93	3	4	214	5	232	3.58e-71	217
MS.gene70062.t1	AT3G04180	55.224	201	87	2	15	213	19	218	6.29e-71	216
MS.gene70062.t1	AT5G38910	49.091	220	68	4	1	215	1	181	1.66e-56	178
MS.gene70062.t1	AT1G02335	44.393	214	108	4	8	215	6	214	3.32e-56	178
MS.gene70062.t1	AT3G62020	46.479	213	105	5	6	215	8	214	1.17e-55	177
MS.gene70062.t1	AT1G09560	45.274	201	103	3	17	215	19	214	7.57e-54	172
MS.gene70062.t1	AT3G05930	47.030	202	98	4	4	202	6	201	4.16e-53	170
MS.gene70062.t1	AT5G26700	49.180	183	79	5	26	204	25	197	6.23e-51	164
MS.gene70062.t1	AT1G18970	45.500	200	100	4	18	215	25	217	2.01e-50	163
MS.gene70062.t1	AT1G18980	45.000	200	101	4	18	215	25	217	2.24e-48	158
MS.gene70062.t1	AT3G62020	47.222	180	89	3	39	215	9	185	6.84e-48	156
MS.gene70062.t1	AT1G10460	40.299	201	112	5	19	214	16	213	1.92e-44	148
MS.gene70062.t1	AT3G10080	40.099	202	112	5	18	215	22	218	1.27e-43	146
MS.gene70062.t1	AT3G10080	40.099	202	112	5	18	215	93	289	6.13e-43	147
MS.gene70062.t1	AT5G61750	41.622	185	103	2	4	186	1	182	5.44e-41	139
MS.gene70062.t1	AT5G20630	41.429	210	111	6	1	208	1	200	4.30e-40	137
MS.gene70062.t1	AT1G72610	40.526	190	104	6	26	213	19	201	3.97e-33	119
MS.gene70062.t1	AT1G74820	37.629	194	112	4	24	215	38	224	7.28e-32	116
MS.gene70062.t1	AT5G38950	55.556	72	32	0	145	216	2	73	6.07e-19	79.0
MS.gene70062.t1	AT5G39100	72.000	50	14	0	7	56	9	58	7.07e-18	75.1

Find 59 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATATACATACCACCATCTT+TGG	0.255130	4.2:-84940864	None:intergenic
TAACACTCTTGGTATATCTT+TGG	0.286436	4.2:+84941116	MS.gene70062:CDS
TTGAGAGCCTAGTGCATTAG+AGG	0.310318	4.2:-84941058	None:intergenic
TTTCAACTGAATGTGGGATA+TGG	0.310660	4.2:+84941327	MS.gene70062:CDS
CCTTGTTAGCATCTTGGCTT+TGG	0.311083	4.2:+84940775	MS.gene70062:CDS
CTTATCCACTTGAAAAGCTT+TGG	0.335788	4.2:-84941454	None:intergenic
TGAAAATGAATAAGACCAAT+TGG	0.356438	4.2:-84941309	None:intergenic
GAGTGCCTTCGAGTACTATA+AGG	0.397511	4.2:-84941203	None:intergenic
ATACTTCCTTGTTAGCATCT+TGG	0.401215	4.2:+84940769	MS.gene70062:CDS
TTCTTCAAACATGTTGAAGC+TGG	0.402098	4.2:+84941030	MS.gene70062:CDS
ATATACATACCACCATCTTT+GGG	0.405165	4.2:-84940863	None:intergenic
GGCAACGCTGTTGCTATTGC+TGG	0.409649	4.2:+84941348	MS.gene70062:CDS
TTGGAGAGGGCTAGGGTCAT+AGG	0.420416	4.2:-84940816	None:intergenic
GATGTGTTTGTGTTCCCAAT+TGG	0.424230	4.2:+84941294	MS.gene70062:CDS
TAAAGAGCCGGTTGTTGTCT+TGG	0.425026	4.2:-84941251	None:intergenic
CAAAAGTCTTGGAGAGGGCT+AGG	0.434671	4.2:-84940824	None:intergenic
ATTATCTTCAAAAGCAGTCT+TGG	0.442184	4.2:+84941487	MS.gene70062:CDS
CTCGAAGGCACTCTTTATGT+TGG	0.443268	4.2:+84941213	MS.gene70062:CDS
TCTTCAGCTTTAACAAGTGC+GGG	0.444792	4.2:-84941006	None:intergenic
GTCACTGTAGACCAACTATT+CGG	0.446134	4.2:+84941090	MS.gene70062:CDS
CATCAAACCAAGACAACAAC+CGG	0.452231	4.2:+84941244	MS.gene70062:CDS
TACTTTGCAGTATTTGTGAA+TGG	0.467591	4.2:+84940967	MS.gene70062:intron
ATAAGGATCTCTGTTCCTCT+AGG	0.468428	4.2:-84941186	None:intergenic
TTTGCAATGGTGATAACTCC+TGG	0.475557	4.2:-84941387	None:intergenic
CTCTAGGGTGGATGTGAGGA+GGG	0.477652	4.2:-84941170	None:intergenic
CTATTCGGACTTAACACTCT+TGG	0.480493	4.2:+84941105	MS.gene70062:CDS
TGATTGCAACACAAAAGTCT+TGG	0.490396	4.2:-84940835	None:intergenic
TCTTCAAACATGTTGAAGCT+GGG	0.501315	4.2:+84941031	MS.gene70062:CDS
GAAAATGAATAAGACCAATT+GGG	0.510497	4.2:-84941308	None:intergenic
AGAACTTCGTCGGAAATAAG+AGG	0.511108	4.2:-84941429	None:intergenic
CCTCTAGGGTGGATGTGAGG+AGG	0.514814	4.2:-84941171	None:intergenic
ACTTTGCAGTATTTGTGAAT+GGG	0.528034	4.2:+84940968	MS.gene70062:intron
TGAGGAGGGTTTAAACCCTT+AGG	0.529214	4.2:-84941156	None:intergenic
GAGTGTTAAGTCCGAATAGT+TGG	0.535207	4.2:-84941101	None:intergenic
ATCTTCAGCTTTAACAAGTG+CGG	0.543612	4.2:-84941007	None:intergenic
CTTTACCAAGGTGCTCAACA+AGG	0.544491	4.2:+84941269	MS.gene70062:CDS
GCAATCAAAGACCCCAAAGA+TGG	0.554146	4.2:+84940851	MS.gene70062:CDS
AGCTTTGGTAAGAACTTCGT+CGG	0.555884	4.2:-84941439	None:intergenic
CCAAAGCCAAGATGCTAACA+AGG	0.556137	4.2:-84940775	None:intergenic
TTTAAACAAAGCATTTGCAA+TGG	0.564041	4.2:-84941400	None:intergenic
AAAAGTCTTGGAGAGGGCTA+GGG	0.564154	4.2:-84940823	None:intergenic
ATCACCCTTGTTGAGCACCT+TGG	0.567836	4.2:-84941274	None:intergenic
TAAGGATCTCTGTTCCTCTA+GGG	0.571738	4.2:-84941185	None:intergenic
GGACTTAGCAGTCAAAATCC+AGG	0.578792	4.2:+84941369	MS.gene70062:CDS
TGAGCACCTTGGTAAAGAGC+CGG	0.579420	4.2:-84941263	None:intergenic
GGATCTCTGTTCCTCTAGGG+TGG	0.590427	4.2:-84941182	None:intergenic
GGGAATGCCTCTAATGCACT+AGG	0.592837	4.2:+84941051	MS.gene70062:CDS
AGGGCTAGGGTCATAGGCAA+TGG	0.604738	4.2:-84940810	None:intergenic
GTTCCTCTAGGGTGGATGTG+AGG	0.620992	4.2:-84941174	None:intergenic
GTCATAGGCAATGGCAACAG+AGG	0.623500	4.2:-84940801	None:intergenic
ATCAAAGACCCCAAAGATGG+TGG	0.628410	4.2:+84940854	MS.gene70062:CDS
TATACATACCACCATCTTTG+GGG	0.639620	4.2:-84940862	None:intergenic
CAACACAAAAGTCTTGGAGA+GGG	0.641422	4.2:-84940829	None:intergenic
GCAACACAAAAGTCTTGGAG+AGG	0.651085	4.2:-84940830	None:intergenic
CCTCCTCACATCCACCCTAG+AGG	0.661653	4.2:+84941171	MS.gene70062:CDS
CTTCAGCTTTAACAAGTGCG+GGG	0.662073	4.2:-84941005	None:intergenic
GAGATCCTTATAGTACTCGA+AGG	0.666965	4.2:+84941198	MS.gene70062:CDS
TTTACCAAGGTGCTCAACAA+GGG	0.669433	4.2:+84941270	MS.gene70062:CDS
CAACAACCGGCTCTTTACCA+AGG	0.674974	4.2:+84941257	MS.gene70062:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GAAAATGAATAAGACCAATT+GGG	-	chr4.2:84941311-84941330	None:intergenic	25.0%
!	GTAATCAAGACTTAAAGTTA+AGG	-	chr4.2:84940892-84940911	None:intergenic	25.0%
!	TGAAAATGAATAAGACCAAT+TGG	-	chr4.2:84941312-84941331	None:intergenic	25.0%
!!	ATTCATTTTCAACTGAATGT+GGG	+	chr4.2:84941321-84941340	MS.gene70062:CDS	25.0%
!!	TATTCATTTTCAACTGAATG+TGG	+	chr4.2:84941320-84941339	MS.gene70062:CDS	25.0%
!!	TTTAAACAAAGCATTTGCAA+TGG	-	chr4.2:84941403-84941422	None:intergenic	25.0%
!!!	AATCATAGTTTTGTTAAACC+AGG	-	chr4.2:84940944-84940963	None:intergenic	25.0%
!!!	ACTCAATTTTTAAGCTTTCA+TGG	+	chr4.2:84940913-84940932	MS.gene70062:intron	25.0%
	ACTTTGCAGTATTTGTGAAT+GGG	+	chr4.2:84940968-84940987	MS.gene70062:intron	30.0%
	ATATACATACCACCATCTTT+GGG	-	chr4.2:84940866-84940885	None:intergenic	30.0%
	ATTATCTTCAAAAGCAGTCT+TGG	+	chr4.2:84941487-84941506	MS.gene70062:CDS	30.0%
	TAACACTCTTGGTATATCTT+TGG	+	chr4.2:84941116-84941135	MS.gene70062:CDS	30.0%
	TACTTTGCAGTATTTGTGAA+TGG	+	chr4.2:84940967-84940986	MS.gene70062:intron	30.0%
	TATATACATACCACCATCTT+TGG	-	chr4.2:84940867-84940886	None:intergenic	30.0%
	ATACTTCCTTGTTAGCATCT+TGG	+	chr4.2:84940769-84940788	MS.gene70062:CDS	35.0%
	CTTATCCACTTGAAAAGCTT+TGG	-	chr4.2:84941457-84941476	None:intergenic	35.0%
	TATACATACCACCATCTTTG+GGG	-	chr4.2:84940865-84940884	None:intergenic	35.0%
	TCTTCAAACATGTTGAAGCT+GGG	+	chr4.2:84941031-84941050	MS.gene70062:CDS	35.0%
	TGATTGCAACACAAAAGTCT+TGG	-	chr4.2:84940838-84940857	None:intergenic	35.0%
	TTCTTCAAACATGTTGAAGC+TGG	+	chr4.2:84941030-84941049	MS.gene70062:CDS	35.0%
	TTTCAACTGAATGTGGGATA+TGG	+	chr4.2:84941327-84941346	MS.gene70062:CDS	35.0%
!	ATCTTCAGCTTTAACAAGTG+CGG	-	chr4.2:84941010-84941029	None:intergenic	35.0%
!!	TCTTACCAAAGCTTTTCAAG+TGG	+	chr4.2:84941449-84941468	MS.gene70062:CDS	35.0%
	AGAACTTCGTCGGAAATAAG+AGG	-	chr4.2:84941432-84941451	None:intergenic	40.0%
	ATAAGGATCTCTGTTCCTCT+AGG	-	chr4.2:84941189-84941208	None:intergenic	40.0%
	CAACACAAAAGTCTTGGAGA+GGG	-	chr4.2:84940832-84940851	None:intergenic	40.0%
	CATCAAACCAAGACAACAAC+CGG	+	chr4.2:84941244-84941263	MS.gene70062:CDS	40.0%
	CTATTCGGACTTAACACTCT+TGG	+	chr4.2:84941105-84941124	MS.gene70062:CDS	40.0%
	GAGATCCTTATAGTACTCGA+AGG	+	chr4.2:84941198-84941217	MS.gene70062:CDS	40.0%
	GAGTGTTAAGTCCGAATAGT+TGG	-	chr4.2:84941104-84941123	None:intergenic	40.0%
	GATGTGTTTGTGTTCCCAAT+TGG	+	chr4.2:84941294-84941313	MS.gene70062:CDS	40.0%
	GTCACTGTAGACCAACTATT+CGG	+	chr4.2:84941090-84941109	MS.gene70062:CDS	40.0%
	TAAGCTTTCATGGTGTTTCC+TGG	+	chr4.2:84940923-84940942	MS.gene70062:intron	40.0%
	TAAGGATCTCTGTTCCTCTA+GGG	-	chr4.2:84941188-84941207	None:intergenic	40.0%
	TTTGCAATGGTGATAACTCC+TGG	-	chr4.2:84941390-84941409	None:intergenic	40.0%
!	AGCTTTGGTAAGAACTTCGT+CGG	-	chr4.2:84941442-84941461	None:intergenic	40.0%
!	TCTTCAGCTTTAACAAGTGC+GGG	-	chr4.2:84941009-84941028	None:intergenic	40.0%
!	TTTACCAAGGTGCTCAACAA+GGG	+	chr4.2:84941270-84941289	MS.gene70062:CDS	40.0%
	AAAAGTCTTGGAGAGGGCTA+GGG	-	chr4.2:84940826-84940845	None:intergenic	45.0%
	ATCAAAGACCCCAAAGATGG+TGG	+	chr4.2:84940854-84940873	MS.gene70062:CDS	45.0%
	CCAAAGCCAAGATGCTAACA+AGG	-	chr4.2:84940778-84940797	None:intergenic	45.0%
	CCTTGTTAGCATCTTGGCTT+TGG	+	chr4.2:84940775-84940794	MS.gene70062:CDS	45.0%
	GAGTGCCTTCGAGTACTATA+AGG	-	chr4.2:84941206-84941225	None:intergenic	45.0%
	GCAACACAAAAGTCTTGGAG+AGG	-	chr4.2:84940833-84940852	None:intergenic	45.0%
	GCAATCAAAGACCCCAAAGA+TGG	+	chr4.2:84940851-84940870	MS.gene70062:CDS	45.0%
	GGACTTAGCAGTCAAAATCC+AGG	+	chr4.2:84941369-84941388	MS.gene70062:CDS	45.0%
	TAAAGAGCCGGTTGTTGTCT+TGG	-	chr4.2:84941254-84941273	None:intergenic	45.0%
	TGAGGAGGGTTTAAACCCTT+AGG	-	chr4.2:84941159-84941178	None:intergenic	45.0%
	TTGAGAGCCTAGTGCATTAG+AGG	-	chr4.2:84941061-84941080	None:intergenic	45.0%
!	CTTCAGCTTTAACAAGTGCG+GGG	-	chr4.2:84941008-84941027	None:intergenic	45.0%
!	CTTTACCAAGGTGCTCAACA+AGG	+	chr4.2:84941269-84941288	MS.gene70062:CDS	45.0%
!!	CTCGAAGGCACTCTTTATGT+TGG	+	chr4.2:84941213-84941232	MS.gene70062:CDS	45.0%
!!!	CGTGTTGATTTTGCGCCTAA+GGG	+	chr4.2:84941141-84941160	MS.gene70062:CDS	45.0%
!!!	TCGTGTTGATTTTGCGCCTA+AGG	+	chr4.2:84941140-84941159	MS.gene70062:CDS	45.0%
	ATCACCCTTGTTGAGCACCT+TGG	-	chr4.2:84941277-84941296	None:intergenic	50.0%
	CAAAAGTCTTGGAGAGGGCT+AGG	-	chr4.2:84940827-84940846	None:intergenic	50.0%
	GGGAATGCCTCTAATGCACT+AGG	+	chr4.2:84941051-84941070	MS.gene70062:CDS	50.0%
	GTCATAGGCAATGGCAACAG+AGG	-	chr4.2:84940804-84940823	None:intergenic	50.0%
	TGAGCACCTTGGTAAAGAGC+CGG	-	chr4.2:84941266-84941285	None:intergenic	50.0%
!	CAACAACCGGCTCTTTACCA+AGG	+	chr4.2:84941257-84941276	MS.gene70062:CDS	50.0%
	AGGGCTAGGGTCATAGGCAA+TGG	-	chr4.2:84940813-84940832	None:intergenic	55.0%
	TTGGAGAGGGCTAGGGTCAT+AGG	-	chr4.2:84940819-84940838	None:intergenic	55.0%
!	GGATCTCTGTTCCTCTAGGG+TGG	-	chr4.2:84941185-84941204	None:intergenic	55.0%
!	GGCAACGCTGTTGCTATTGC+TGG	+	chr4.2:84941348-84941367	MS.gene70062:CDS	55.0%
!!	CTCTAGGGTGGATGTGAGGA+GGG	-	chr4.2:84941173-84941192	None:intergenic	55.0%
!!	GTTCCTCTAGGGTGGATGTG+AGG	-	chr4.2:84941177-84941196	None:intergenic	55.0%
	CCTCCTCACATCCACCCTAG+AGG	+	chr4.2:84941171-84941190	MS.gene70062:CDS	60.0%
!!	CCTCTAGGGTGGATGTGAGG+AGG	-	chr4.2:84941174-84941193	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	84940758	84941524	84940758	ID=MS.gene70062
chr4.2	mRNA	84940758	84941524	84940758	ID=MS.gene70062.t1;Parent=MS.gene70062
chr4.2	exon	84940758	84940875	84940758	ID=MS.gene70062.t1.exon1;Parent=MS.gene70062.t1
chr4.2	CDS	84940758	84940875	84940758	ID=cds.MS.gene70062.t1;Parent=MS.gene70062.t1
chr4.2	exon	84940977	84941524	84940977	ID=MS.gene70062.t1.exon2;Parent=MS.gene70062.t1
chr4.2	CDS	84940977	84941524	84940977	ID=cds.MS.gene70062.t1;Parent=MS.gene70062.t1

Gene Sequence

>MS.gene70062

ATGAAGACTATATACTTCCTTGTTAGCATCTTGGCTTTGGCATCCTCTGTTGCCATTGCCTATGACCCTAGCCCTCTCCAAGACTTTTGTGTTGCAATCAAAGACCCCAAAGATGGTGGTATGTATATATTCCTTAACTTTAAGTCTTGATTACTACTCAATTTTTAAGCTTTCATGGTGTTTCCTGGTTTAACAAAACTATGATTTGATACTTTGCAGTATTTGTGAATGGGAAATTCTGTAAAGACCCCGCACTTGTTAAAGCTGAAGATTTCTTCAAACATGTTGAAGCTGGGAATGCCTCTAATGCACTAGGCTCTCAAGTAACTCAAGTCACTGTAGACCAACTATTCGGACTTAACACTCTTGGTATATCTTTGGCTCGTGTTGATTTTGCGCCTAAGGGTTTAAACCCTCCTCACATCCACCCTAGAGGAACAGAGATCCTTATAGTACTCGAAGGCACTCTTTATGTTGGATTTGTCTCATCAAACCAAGACAACAACCGGCTCTTTACCAAGGTGCTCAACAAGGGTGATGTGTTTGTGTTCCCAATTGGTCTTATTCATTTTCAACTGAATGTGGGATATGGCAACGCTGTTGCTATTGCTGGACTTAGCAGTCAAAATCCAGGAGTTATCACCATTGCAAATGCTTTGTTTAAATCTGATCCTCTTATTTCCGACGAAGTTCTTACCAAAGCTTTTCAAGTGGATAAGAGCATAATTGATTATCTTCAAAAGCAGTCTTGGTATGACAACAACTAG

Protein sequence

>MS.gene70062.t1

MKTIYFLVSILALASSVAIAYDPSPLQDFCVAIKDPKDGVFVNGKFCKDPALVKAEDFFKHVEAGNASNALGSQVTQVTVDQLFGLNTLGISLARVDFAPKGLNPPHIHPRGTEILIVLEGTLYVGFVSSNQDNNRLFTKVLNKGDVFVFPIGLIHFQLNVGYGNAVAIAGLSSQNPGVITIANALFKSDPLISDEVLTKAFQVDKSIIDYLQKQSWYDNN