AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene037518

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037518.t1 MTR_5g046410 97.297 222 5 1 1 222 1 221 1.31e-156 432
MS.gene037518.t1 MTR_5g046430 97.297 222 5 1 1 222 1 221 1.31e-156 432
MS.gene037518.t1 MTR_4g017030 97.297 222 5 1 1 222 1 221 1.31e-156 432
MS.gene037518.t1 MTR_4g017050 96.847 222 6 1 1 222 1 221 4.58e-156 431
MS.gene037518.t1 MTR_2g019250 96.396 222 7 1 1 222 1 221 5.96e-156 431
MS.gene037518.t1 MTR_4g017040 95.946 222 8 1 1 222 1 221 1.24e-154 427
MS.gene037518.t1 MTR_1g079490 79.372 223 44 2 1 222 1 222 6.31e-126 355
MS.gene037518.t1 MTR_6g005360 75.225 222 53 1 1 222 1 220 3.02e-118 335
MS.gene037518.t1 MTR_6g005340 74.775 222 54 1 1 222 1 220 4.00e-117 333
MS.gene037518.t1 MTR_6g005310 74.661 221 54 1 1 221 1 219 1.21e-116 332
MS.gene037518.t1 MTR_6g005330 75.785 223 50 3 1 222 1 220 1.46e-116 331
MS.gene037518.t1 MTR_6g005350 61.712 222 82 2 1 222 1 219 5.26e-90 264
MS.gene037518.t1 MTR_6g005380 61.244 209 80 1 1 209 1 208 9.41e-88 258
MS.gene037518.t1 MTR_1g023770 58.716 218 78 3 1 216 1 208 1.12e-79 238
MS.gene037518.t1 MTR_2g030855 59.701 201 70 4 19 216 19 211 1.78e-76 229
MS.gene037518.t1 MTR_2g031270 58.911 202 71 5 19 216 19 212 4.82e-72 218
MS.gene037518.t1 MTR_2g030895 63.218 174 57 4 47 216 7 177 6.11e-70 212
MS.gene037518.t1 MTR_2g030865 62.644 174 58 4 47 216 7 177 5.62e-68 207
MS.gene037518.t1 MTR_1g085500 46.296 216 108 3 3 216 4 213 3.80e-61 191
MS.gene037518.t1 MTR_1g085140 45.771 201 101 3 18 216 20 214 1.73e-53 171
MS.gene037518.t1 MTR_7g111240 47.208 197 96 3 22 216 20 210 5.70e-52 167
MS.gene037518.t1 MTR_7g111240 47.208 197 93 3 22 216 20 207 1.27e-50 164
MS.gene037518.t1 MTR_2g031300 53.488 172 54 4 47 215 7 155 2.69e-50 161
MS.gene037518.t1 MTR_5g036570 44.949 198 100 4 22 216 27 218 3.55e-48 157
MS.gene037518.t1 MTR_1g112200 40.092 217 113 6 2 216 5 206 6.20e-44 146
MS.gene037518.t1 MTR_1g060120 36.036 222 129 5 2 216 5 220 1.32e-40 139
MS.gene037518.t1 MTR_2g041550 43.386 189 97 4 28 214 23 203 6.07e-40 136
MS.gene037518.t1 MTR_2g041570 37.674 215 116 5 3 215 6 204 5.43e-38 131
MS.gene037518.t1 MTR_2g041580 37.850 214 115 5 3 214 6 203 6.31e-38 131
MS.gene037518.t1 MTR_8g007300 40.299 201 106 4 22 220 26 214 2.01e-36 127
MS.gene037518.t1 MTR_8g020750 39.899 198 98 5 1 191 1 184 1.18e-33 120
MS.gene037518.t1 MTR_3g019710 39.691 194 106 5 7 198 3 187 4.76e-33 119
MS.gene037518.t1 MTR_8g020590 39.583 192 106 5 26 215 19 202 1.77e-32 117
MS.gene037518.t1 MTR_8g020630 38.636 176 98 4 7 180 3 170 3.03e-32 116
MS.gene037518.t1 MTR_8g020650 35.751 193 112 5 26 216 22 204 3.01e-30 111
MS.gene037518.t1 MTR_2g036120 36.792 212 117 6 1 207 1 200 5.77e-30 110
MS.gene037518.t1 MTR_8g020770 40.556 180 94 4 1 177 1 170 2.43e-29 109
MS.gene037518.t1 MTR_3g019650 40.000 175 94 5 26 198 21 186 2.48e-29 108
MS.gene037518.t1 MTR_8g020670 33.945 218 128 6 1 216 1 204 2.92e-29 108
MS.gene037518.t1 MTR_3g019680 40.000 175 94 5 26 198 21 186 5.82e-29 108
MS.gene037518.t1 MTR_0284s0010 36.893 206 117 5 7 207 3 200 1.46e-28 107
MS.gene037518.t1 MTR_2g044040 36.782 174 100 4 7 178 3 168 1.92e-28 106
MS.gene037518.t1 MTR_2g086620 42.424 132 69 3 85 215 77 202 6.63e-28 105
MS.gene037518.t1 MTR_2g045100 41.290 155 81 4 26 178 22 168 7.05e-28 105
MS.gene037518.t1 MTR_4g052780 36.098 205 104 6 16 215 21 203 7.63e-28 105
MS.gene037518.t1 MTR_3g096330 35.784 204 123 4 5 206 560 757 1.28e-27 110
MS.gene037518.t1 MTR_4g052770 35.610 205 105 6 16 215 21 203 1.36e-27 104
MS.gene037518.t1 MTR_2g086640 34.975 203 106 6 17 215 21 201 1.93e-27 103
MS.gene037518.t1 MTR_2g086630 41.667 132 70 3 85 215 77 202 2.30e-27 103
MS.gene037518.t1 MTR_5g031460 35.567 194 114 4 18 207 14 200 3.19e-26 100
MS.gene037518.t1 MTR_7g011340 37.433 187 104 5 26 207 22 200 1.26e-24 97.1
MS.gene037518.t1 MTR_4g010340 37.143 175 101 4 43 216 34 200 1.56e-24 96.7
MS.gene037518.t1 MTR_2g019780 32.727 220 131 7 1 216 1 207 2.82e-24 95.9
MS.gene037518.t1 MTR_8g020760 33.486 218 131 6 1 216 1 206 5.36e-24 95.1
MS.gene037518.t1 MTR_8g020763 36.471 170 98 3 43 207 17 181 8.14e-24 94.4
MS.gene037518.t1 MTR_3g073170 34.896 192 109 6 28 216 27 205 2.45e-23 93.6
MS.gene037518.t1 MTR_8g020610 32.381 210 130 6 7 214 1 200 4.59e-23 92.8
MS.gene037518.t1 MTR_2g030915 48.052 77 24 1 93 169 1 61 1.78e-17 75.1
MS.gene037518.t1 MTR_3g019810 32.500 200 116 8 7 203 109 292 6.17e-17 78.2
MS.gene037518.t1 MTR_4g027415 38.760 129 69 4 26 152 22 142 6.90e-17 74.7
MS.gene037518.t1 MTR_2g072560 36.885 122 68 4 1 120 1 115 5.26e-13 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037518.t1 AT3G05950 65.179 224 69 5 6 222 7 228 1.80e-94 276
MS.gene037518.t1 AT5G39150 62.557 219 78 3 1 216 1 218 4.20e-92 270
MS.gene037518.t1 AT5G39180 62.557 219 78 3 1 216 1 218 6.58e-92 269
MS.gene037518.t1 AT5G39120 61.927 218 79 3 1 216 3 218 1.49e-91 268
MS.gene037518.t1 AT5G39160 63.182 220 75 4 1 216 1 218 3.13e-90 265
MS.gene037518.t1 AT5G39190 63.182 220 75 4 1 216 1 218 6.22e-90 264
MS.gene037518.t1 AT5G39110 62.326 215 78 2 4 216 6 219 6.79e-90 264
MS.gene037518.t1 AT5G39130 61.818 220 78 4 1 216 1 218 2.42e-89 263
MS.gene037518.t1 AT5G39160 60.909 220 76 4 1 216 1 214 3.18e-85 252
MS.gene037518.t1 AT4G14630 59.545 220 84 3 1 216 3 221 5.10e-85 251
MS.gene037518.t1 AT3G04200 61.244 209 77 4 8 214 13 219 2.80e-81 242
MS.gene037518.t1 AT5G38910 59.729 221 81 4 1 216 1 218 6.88e-79 236
MS.gene037518.t1 AT5G39190 57.339 218 69 3 1 216 1 196 8.56e-78 232
MS.gene037518.t1 AT5G39160 56.881 218 70 3 1 216 1 196 2.03e-77 231
MS.gene037518.t1 AT5G38940 60.406 197 74 2 22 216 26 220 2.22e-77 232
MS.gene037518.t1 AT5G38930 61.307 199 73 2 20 216 24 220 2.24e-77 232
MS.gene037518.t1 AT5G38940 60.406 197 74 2 22 216 50 244 3.29e-77 233
MS.gene037518.t1 AT5G38960 59.901 202 76 3 18 216 22 221 4.51e-77 231
MS.gene037518.t1 AT3G04170 54.762 210 88 4 8 215 12 216 3.61e-72 219
MS.gene037518.t1 AT3G04190 54.950 202 87 3 15 214 19 218 2.10e-71 217
MS.gene037518.t1 AT3G04150 53.953 215 92 4 6 215 7 219 3.26e-71 217
MS.gene037518.t1 AT3G04150 51.101 227 93 4 6 215 7 232 2.97e-69 212
MS.gene037518.t1 AT3G04180 53.465 202 90 3 15 214 19 218 2.06e-68 209
MS.gene037518.t1 AT1G02335 46.231 199 99 3 20 216 22 214 7.92e-56 177
MS.gene037518.t1 AT5G38910 48.416 221 69 4 1 216 1 181 5.79e-55 174
MS.gene037518.t1 AT3G62020 45.794 214 106 5 6 216 8 214 9.93e-55 174
MS.gene037518.t1 AT1G09560 45.050 202 103 3 17 216 19 214 2.59e-53 171
MS.gene037518.t1 AT3G05930 47.500 200 95 4 7 203 9 201 1.99e-52 169
MS.gene037518.t1 AT5G26700 48.913 184 79 5 26 205 25 197 1.73e-50 163
MS.gene037518.t1 AT1G18970 44.279 201 102 4 18 216 25 217 9.04e-49 159
MS.gene037518.t1 AT1G18980 44.776 201 101 4 18 216 25 217 1.56e-47 156
MS.gene037518.t1 AT3G62020 46.409 181 90 3 39 216 9 185 3.34e-47 154
MS.gene037518.t1 AT1G10460 41.709 199 107 5 22 215 19 213 1.22e-44 149
MS.gene037518.t1 AT3G10080 39.409 203 113 5 18 216 22 218 3.79e-43 145
MS.gene037518.t1 AT3G10080 39.409 203 113 5 18 216 93 289 1.66e-42 145
MS.gene037518.t1 AT5G61750 41.848 184 101 2 6 187 3 182 2.63e-40 137
MS.gene037518.t1 AT5G20630 40.284 211 113 6 1 209 1 200 5.52e-39 134
MS.gene037518.t1 AT1G72610 39.267 191 106 6 26 214 19 201 1.05e-31 115
MS.gene037518.t1 AT1G74820 38.144 194 110 4 25 216 39 224 1.22e-31 115
MS.gene037518.t1 AT5G38950 54.167 72 33 0 146 217 2 73 1.24e-18 78.2
MS.gene037518.t1 AT5G39100 67.925 53 17 0 4 56 6 58 5.90e-18 75.1

Find 58 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATATACATACCACCATCTT+TGG 0.255130 4.3:-82522440 None:intergenic
TTTCAACTGAATGTTGGATA+TGG 0.274546 4.3:+82523018 MS.gene037518:CDS
TAACACTCTTGGTATATCTT+TGG 0.286436 4.3:+82522804 MS.gene037518:CDS
CCTTGTTAGCATCCTGGCTT+TGG 0.313379 4.3:+82522351 MS.gene037518:CDS
CTTATCCACTTGAAAAGCTT+TGG 0.335788 4.3:-82523145 None:intergenic
TGAAAATGAATAAGACCAAT+TGG 0.356438 4.3:-82523000 None:intergenic
GAGTGCCTTCAAGTACTATA+AGG 0.386700 4.3:-82522891 None:intergenic
TTCTTCAAACATGTTGAAGC+TGG 0.402098 4.3:+82522718 MS.gene037518:CDS
ATATACATACCACCATCTTT+GGG 0.405165 4.3:-82522439 None:intergenic
CTCTAGGGTGGATGTGAGGT+GGG 0.411548 4.3:-82522858 None:intergenic
TTGGAGAGGGCTAGGGTCAT+AGG 0.420416 4.3:-82522392 None:intergenic
GATGTGTTTGTGTTCCCAAT+TGG 0.424230 4.3:+82522985 MS.gene037518:CDS
GGCAACGCGGTTGCTATCGC+TGG 0.429748 4.3:+82523039 MS.gene037518:CDS
CAAAAGTCTTGGAGAGGGCT+AGG 0.434671 4.3:-82522400 None:intergenic
CATCCTGGCTTTGGCATCCT+CGG 0.435136 4.3:+82522360 MS.gene037518:CDS
ATTATCTTCAAAAGCAGTCT+TGG 0.442184 4.3:+82523178 MS.gene037518:CDS
TCTTCAGCTTTAACAAGTGC+GGG 0.444792 4.3:-82522694 None:intergenic
GTCACTGTAGACCAACTATT+CGG 0.446134 4.3:+82522778 MS.gene037518:CDS
CTTGAAGGCACTCTTTATGT+TGG 0.454430 4.3:+82522901 MS.gene037518:CDS
TACTTTGCAGTATTTGTGAA+TGG 0.463825 4.3:+82522655 MS.gene037518:intron
TTTGCAACTGTGATAACTCC+TGG 0.466515 4.3:-82523078 None:intergenic
ATAAGGATCTCTGTTCCTCT+AGG 0.468428 4.3:-82522874 None:intergenic
GAGATCCTTATAGTACTTGA+AGG 0.469573 4.3:+82522886 MS.gene037518:CDS
CTATTCGGACTTAACACTCT+TGG 0.480493 4.3:+82522793 MS.gene037518:CDS
TGATTGCAACACAAAAGTCT+TGG 0.490396 4.3:-82522411 None:intergenic
CACTTGAGAGCCTAGAGCGT+TGG 0.493916 4.3:-82522749 None:intergenic
ATACTTCCTTGTTAGCATCC+TGG 0.496774 4.3:+82522345 MS.gene037518:CDS
CCTCTAGGGTGGATGTGAGG+TGG 0.496940 4.3:-82522859 None:intergenic
TTGAGAGCCTAGAGCGTTGG+AGG 0.499847 4.3:-82522746 None:intergenic
TCTTCAAACATGTTGAAGCT+GGG 0.501315 4.3:+82522719 MS.gene037518:CDS
CAACCGAGGATGCCAAAGCC+AGG 0.505056 4.3:-82522363 None:intergenic
GAAAATGAATAAGACCAATT+GGG 0.510497 4.3:-82522999 None:intergenic
AGAACTTCGTCGGAAATAAG+AGG 0.511108 4.3:-82523120 None:intergenic
CCAAAGCCAGGATGCTAACA+AGG 0.515624 4.3:-82522351 None:intergenic
GGGAATGCCTCCAACGCTCT+AGG 0.530753 4.3:+82522739 MS.gene037518:CDS
GAGTGTTAAGTCCGAATAGT+TGG 0.535207 4.3:-82522789 None:intergenic
ATCTTCAGCTTTAACAAGTG+CGG 0.543612 4.3:-82522695 None:intergenic
CTTTACCAAGGTGCTCAACA+AGG 0.544491 4.3:+82522960 MS.gene037518:CDS
TGAGGTGGGTTTAAACCCTT+AGG 0.548128 4.3:-82522844 None:intergenic
GCAATCAAAGACCCCAAAGA+TGG 0.554146 4.3:+82522427 MS.gene037518:CDS
AGCTTTGGTAAGAACTTCGT+CGG 0.555884 4.3:-82523130 None:intergenic
AAAAGTCTTGGAGAGGGCTA+GGG 0.564154 4.3:-82522399 None:intergenic
ATCACCCTTGTTGAGCACCT+TGG 0.567836 4.3:-82522965 None:intergenic
TAAGGATCTCTGTTCCTCTA+GGG 0.571738 4.3:-82522873 None:intergenic
GGACTTAGCAGTCAAAATCC+AGG 0.578792 4.3:+82523060 MS.gene037518:CDS
GGATCTCTGTTCCTCTAGGG+TGG 0.590427 4.3:-82522870 None:intergenic
AGGGCTAGGGTCATAGGCAA+AGG 0.609226 4.3:-82522386 None:intergenic
ATCAAAGACCCCAAAGATGG+TGG 0.628410 4.3:+82522430 MS.gene037518:CDS
TATACATACCACCATCTTTG+GGG 0.639620 4.3:-82522438 None:intergenic
CAACACAAAAGTCTTGGAGA+GGG 0.641422 4.3:-82522405 None:intergenic
GTTCCTCTAGGGTGGATGTG+AGG 0.650118 4.3:-82522862 None:intergenic
GCAACACAAAAGTCTTGGAG+AGG 0.651085 4.3:-82522406 None:intergenic
GTCATAGGCAAAGGCAACCG+AGG 0.652490 4.3:-82522377 None:intergenic
TTTACCAAGGTGCTCAACAA+GGG 0.659466 4.3:+82522961 MS.gene037518:CDS
CCACCTCACATCCACCCTAG+AGG 0.660499 4.3:+82522859 MS.gene037518:CDS
CTTCAGCTTTAACAAGTGCG+GGG 0.662073 4.3:-82522693 None:intergenic
CAATAATCGTCTCTTTACCA+AGG 0.672342 4.3:+82522948 MS.gene037518:CDS
GAATGTTGGATATGGCAACG+CGG 0.840401 4.3:+82523026 MS.gene037518:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTACAAATATTAGACTTAT+TGG + chr4.3:82522522-82522541 MS.gene037518:intron 20.0%
!!! AGATATAGAATCTTTTGTTT+TGG + chr4.3:82522594-82522613 MS.gene037518:intron 20.0%
! AATCATAGTTTAGTTAAACC+AGG - chr4.3:82522632-82522651 None:intergenic 25.0%
! CAAAAGATTCTATATCTACA+TGG - chr4.3:82522591-82522610 None:intergenic 25.0%
! GAAAATGAATAAGACCAATT+GGG - chr4.3:82523002-82523021 None:intergenic 25.0%
! GTAATCAAGACTTAAAGTTA+AGG - chr4.3:82522468-82522487 None:intergenic 25.0%
! TGAAAATGAATAAGACCAAT+TGG - chr4.3:82523003-82523022 None:intergenic 25.0%
!! ATTCATTTTCAACTGAATGT+TGG + chr4.3:82523012-82523031 MS.gene037518:CDS 25.0%
ATATACATACCACCATCTTT+GGG - chr4.3:82522442-82522461 None:intergenic 30.0%
ATTATCTTCAAAAGCAGTCT+TGG + chr4.3:82523178-82523197 MS.gene037518:CDS 30.0%
TAACACTCTTGGTATATCTT+TGG + chr4.3:82522804-82522823 MS.gene037518:CDS 30.0%
TACTTTGCAGTATTTGTGAA+TGG + chr4.3:82522655-82522674 MS.gene037518:intron 30.0%
TATATACATACCACCATCTT+TGG - chr4.3:82522443-82522462 None:intergenic 30.0%
TTTCAACTGAATGTTGGATA+TGG + chr4.3:82523018-82523037 MS.gene037518:CDS 30.0%
!!! GAATCTTTTGTTTTGGTTCA+TGG + chr4.3:82522601-82522620 MS.gene037518:intron 30.0%
CAATAATCGTCTCTTTACCA+AGG + chr4.3:82522948-82522967 MS.gene037518:CDS 35.0%
CTTATCCACTTGAAAAGCTT+TGG - chr4.3:82523148-82523167 None:intergenic 35.0%
GAGATCCTTATAGTACTTGA+AGG + chr4.3:82522886-82522905 MS.gene037518:CDS 35.0%
TATACATACCACCATCTTTG+GGG - chr4.3:82522441-82522460 None:intergenic 35.0%
TCTTCAAACATGTTGAAGCT+GGG + chr4.3:82522719-82522738 MS.gene037518:CDS 35.0%
TGATTGCAACACAAAAGTCT+TGG - chr4.3:82522414-82522433 None:intergenic 35.0%
TTCTTCAAACATGTTGAAGC+TGG + chr4.3:82522718-82522737 MS.gene037518:CDS 35.0%
! ATCTTCAGCTTTAACAAGTG+CGG - chr4.3:82522698-82522717 None:intergenic 35.0%
!! TCTTACCAAAGCTTTTCAAG+TGG + chr4.3:82523140-82523159 MS.gene037518:CDS 35.0%
AGAACTTCGTCGGAAATAAG+AGG - chr4.3:82523123-82523142 None:intergenic 40.0%
ATAAGGATCTCTGTTCCTCT+AGG - chr4.3:82522877-82522896 None:intergenic 40.0%
ATACTTCCTTGTTAGCATCC+TGG + chr4.3:82522345-82522364 MS.gene037518:CDS 40.0%
CAACACAAAAGTCTTGGAGA+GGG - chr4.3:82522408-82522427 None:intergenic 40.0%
CTATTCGGACTTAACACTCT+TGG + chr4.3:82522793-82522812 MS.gene037518:CDS 40.0%
GAGTGCCTTCAAGTACTATA+AGG - chr4.3:82522894-82522913 None:intergenic 40.0%
GAGTGTTAAGTCCGAATAGT+TGG - chr4.3:82522792-82522811 None:intergenic 40.0%
GATGTGTTTGTGTTCCCAAT+TGG + chr4.3:82522985-82523004 MS.gene037518:CDS 40.0%
GTCACTGTAGACCAACTATT+CGG + chr4.3:82522778-82522797 MS.gene037518:CDS 40.0%
TAAGGATCTCTGTTCCTCTA+GGG - chr4.3:82522876-82522895 None:intergenic 40.0%
TTTGCAACTGTGATAACTCC+TGG - chr4.3:82523081-82523100 None:intergenic 40.0%
! AGCTTTGGTAAGAACTTCGT+CGG - chr4.3:82523133-82523152 None:intergenic 40.0%
! TCTTCAGCTTTAACAAGTGC+GGG - chr4.3:82522697-82522716 None:intergenic 40.0%
! TTTACCAAGGTGCTCAACAA+GGG + chr4.3:82522961-82522980 MS.gene037518:CDS 40.0%
!! CTTGAAGGCACTCTTTATGT+TGG + chr4.3:82522901-82522920 MS.gene037518:CDS 40.0%
!!! CGTGTTGATTTTGTGCCTAA+GGG + chr4.3:82522829-82522848 MS.gene037518:CDS 40.0%
!!! TCGTGTTGATTTTGTGCCTA+AGG + chr4.3:82522828-82522847 MS.gene037518:CDS 40.0%
!!! TTTTGGTTCATGGTGTTTCC+TGG + chr4.3:82522611-82522630 MS.gene037518:intron 40.0%
AAAAGTCTTGGAGAGGGCTA+GGG - chr4.3:82522402-82522421 None:intergenic 45.0%
ATCAAAGACCCCAAAGATGG+TGG + chr4.3:82522430-82522449 MS.gene037518:CDS 45.0%
GAATGTTGGATATGGCAACG+CGG + chr4.3:82523026-82523045 MS.gene037518:CDS 45.0%
GCAACACAAAAGTCTTGGAG+AGG - chr4.3:82522409-82522428 None:intergenic 45.0%
GCAATCAAAGACCCCAAAGA+TGG + chr4.3:82522427-82522446 MS.gene037518:CDS 45.0%
GGACTTAGCAGTCAAAATCC+AGG + chr4.3:82523060-82523079 MS.gene037518:CDS 45.0%
! CTTCAGCTTTAACAAGTGCG+GGG - chr4.3:82522696-82522715 None:intergenic 45.0%
! CTTTACCAAGGTGCTCAACA+AGG + chr4.3:82522960-82522979 MS.gene037518:CDS 45.0%
! TGAGGTGGGTTTAAACCCTT+AGG - chr4.3:82522847-82522866 None:intergenic 45.0%
!!! AAAAAAAAAAAAAACATTTT+AGG - chr4.3:82522556-82522575 None:intergenic 5.0%
!!! AAAAAAAAAAAAACATTTTA+GGG - chr4.3:82522555-82522574 None:intergenic 5.0%
!!! AATGTTTTTTTTTTTTTTTT+TGG + chr4.3:82522558-82522577 MS.gene037518:intron 5.0%
ATCACCCTTGTTGAGCACCT+TGG - chr4.3:82522968-82522987 None:intergenic 50.0%
CAAAAGTCTTGGAGAGGGCT+AGG - chr4.3:82522403-82522422 None:intergenic 50.0%
CCAAAGCCAGGATGCTAACA+AGG - chr4.3:82522354-82522373 None:intergenic 50.0%
CCTTGTTAGCATCCTGGCTT+TGG + chr4.3:82522351-82522370 MS.gene037518:CDS 50.0%
AGGGCTAGGGTCATAGGCAA+AGG - chr4.3:82522389-82522408 None:intergenic 55.0%
CACTTGAGAGCCTAGAGCGT+TGG - chr4.3:82522752-82522771 None:intergenic 55.0%
GTCATAGGCAAAGGCAACCG+AGG - chr4.3:82522380-82522399 None:intergenic 55.0%
TTGGAGAGGGCTAGGGTCAT+AGG - chr4.3:82522395-82522414 None:intergenic 55.0%
! GGATCTCTGTTCCTCTAGGG+TGG - chr4.3:82522873-82522892 None:intergenic 55.0%
!! CATCCTGGCTTTGGCATCCT+CGG + chr4.3:82522360-82522379 MS.gene037518:CDS 55.0%
!! CTCTAGGGTGGATGTGAGGT+GGG - chr4.3:82522861-82522880 None:intergenic 55.0%
!! GTTCCTCTAGGGTGGATGTG+AGG - chr4.3:82522865-82522884 None:intergenic 55.0%
!! TTGAGAGCCTAGAGCGTTGG+AGG - chr4.3:82522749-82522768 None:intergenic 55.0%
CAACCGAGGATGCCAAAGCC+AGG - chr4.3:82522366-82522385 None:intergenic 60.0%
CCACCTCACATCCACCCTAG+AGG + chr4.3:82522859-82522878 MS.gene037518:CDS 60.0%
GGGAATGCCTCCAACGCTCT+AGG + chr4.3:82522739-82522758 MS.gene037518:CDS 60.0%
!! CCTCTAGGGTGGATGTGAGG+TGG - chr4.3:82522862-82522881 None:intergenic 60.0%
GGCAACGCGGTTGCTATCGC+TGG + chr4.3:82523039-82523058 MS.gene037518:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 82522334 82523215 82522334 ID=MS.gene037518
chr4.3 mRNA 82522334 82523215 82522334 ID=MS.gene037518.t1;Parent=MS.gene037518
chr4.3 exon 82522334 82522451 82522334 ID=MS.gene037518.t1.exon1;Parent=MS.gene037518.t1
chr4.3 CDS 82522334 82522451 82522334 ID=cds.MS.gene037518.t1;Parent=MS.gene037518.t1
chr4.3 exon 82522665 82523215 82522665 ID=MS.gene037518.t1.exon2;Parent=MS.gene037518.t1
chr4.3 CDS 82522665 82523215 82522665 ID=cds.MS.gene037518.t1;Parent=MS.gene037518.t1
Gene Sequence

>MS.gene037518

ATGAAGACTACATACTTCCTTGTTAGCATCCTGGCTTTGGCATCCTCGGTTGCCTTTGCCTATGACCCTAGCCCTCTCCAAGACTTTTGTGTTGCAATCAAAGACCCCAAAGATGGTGGTATGTATATATTCCTTAACTTTAAGTCTTGATTACTACTCAATTTTTAAGCTAACATATGAAAATGTTCTCTACAAATATTAGACTTATTGGTTTTAATCCCTAAAATGTTTTTTTTTTTTTTTTTGGTTTTAGTCCATGTAGATATAGAATCTTTTGTTTTGGTTCATGGTGTTTCCTGGTTTAACTAAACTATGATTTGATACTTTGCAGTATTTGTGAATGGAAAATTCTGTAAAGACCCCGCACTTGTTAAAGCTGAAGATTTCTTCAAACATGTTGAAGCTGGGAATGCCTCCAACGCTCTAGGCTCTCAAGTGACTCAAGTCACTGTAGACCAACTATTCGGACTTAACACTCTTGGTATATCTTTGGCTCGTGTTGATTTTGTGCCTAAGGGTTTAAACCCACCTCACATCCACCCTAGAGGAACAGAGATCCTTATAGTACTTGAAGGCACTCTTTATGTTGGATTTGTCACGTCAAATCAAGACAACAATAATCGTCTCTTTACCAAGGTGCTCAACAAGGGTGATGTGTTTGTGTTCCCAATTGGTCTTATTCATTTTCAACTGAATGTTGGATATGGCAACGCGGTTGCTATCGCTGGACTTAGCAGTCAAAATCCAGGAGTTATCACAGTTGCAAATGCTTTGTTTAAATCTGATCCTCTTATTTCCGACGAAGTTCTTACCAAAGCTTTTCAAGTGGATAAGAGCATAATTGATTATCTTCAAAAGCAGTCTTGGTATGACAACAACTAG
Protein sequence

>MS.gene037518.t1

MKTTYFLVSILALASSVAFAYDPSPLQDFCVAIKDPKDGVFVNGKFCKDPALVKAEDFFKHVEAGNASNALGSQVTQVTVDQLFGLNTLGISLARVDFVPKGLNPPHIHPRGTEILIVLEGTLYVGFVTSNQDNNNRLFTKVLNKGDVFVFPIGLIHFQLNVGYGNAVAIAGLSSQNPGVITVANALFKSDPLISDEVLTKAFQVDKSIIDYLQKQSWYDNN