Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89775.t1 | XP_003604729.1 | 98.2 | 222 | 3 | 1 | 1 | 222 | 1 | 221 | 2.50E-117 | 431.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89775.t1 | Q9SFF9 | 65.2 | 224 | 69 | 5 | 6 | 222 | 7 | 228 | 2.1e-72 | 273.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89775.t1 | G7JQ50 | 98.2 | 222 | 3 | 1 | 1 | 222 | 1 | 221 | 1.8e-117 | 431.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050971 | MS.gene89775 | -0.80868 | 2.75E-50 | -1.69E-46 |
| MS.gene051158 | MS.gene89775 | 0.806668 | 7.36E-50 | -1.69E-46 |
| MS.gene052188 | MS.gene89775 | -0.808198 | 3.48E-50 | -1.69E-46 |
| MS.gene05240 | MS.gene89775 | 0.820769 | 5.76E-53 | -1.69E-46 |
| MS.gene05244 | MS.gene89775 | 0.805592 | 1.24E-49 | -1.69E-46 |
| MS.gene053031 | MS.gene89775 | 0.817499 | 3.19E-52 | -1.69E-46 |
| MS.gene054181 | MS.gene89775 | 0.810412 | 1.17E-50 | -1.69E-46 |
| MS.gene054290 | MS.gene89775 | 0.832933 | 7.13E-56 | -1.69E-46 |
| MS.gene055601 | MS.gene89775 | 0.834131 | 3.58E-56 | -1.69E-46 |
| MS.gene055959 | MS.gene89775 | 0.802753 | 4.83E-49 | -1.69E-46 |
| MS.gene056899 | MS.gene89775 | 0.825675 | 4.12E-54 | -1.69E-46 |
| MS.gene057544 | MS.gene89775 | 0.809775 | 1.60E-50 | -1.69E-46 |
| MS.gene058218 | MS.gene89775 | 0.834622 | 2.69E-56 | -1.69E-46 |
| MS.gene058928 | MS.gene89775 | -0.810066 | 1.39E-50 | -1.69E-46 |
| MS.gene059043 | MS.gene89775 | 0.815551 | 8.72E-52 | -1.69E-46 |
| MS.gene059294 | MS.gene89775 | -0.849137 | 3.92E-60 | -1.69E-46 |
| MS.gene059402 | MS.gene89775 | 0.854967 | 8.65E-62 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89775.t1 | MTR_5g046410 | 98.198 | 222 | 3 | 1 | 1 | 222 | 1 | 221 | 3.87e-158 | 436 |
| MS.gene89775.t1 | MTR_5g046430 | 98.198 | 222 | 3 | 1 | 1 | 222 | 1 | 221 | 3.87e-158 | 436 |
| MS.gene89775.t1 | MTR_4g017030 | 98.198 | 222 | 3 | 1 | 1 | 222 | 1 | 221 | 3.87e-158 | 436 |
| MS.gene89775.t1 | MTR_4g017050 | 97.748 | 222 | 4 | 1 | 1 | 222 | 1 | 221 | 1.32e-157 | 435 |
| MS.gene89775.t1 | MTR_2g019250 | 95.946 | 222 | 8 | 1 | 1 | 222 | 1 | 221 | 6.23e-156 | 431 |
| MS.gene89775.t1 | MTR_4g017040 | 96.396 | 222 | 7 | 1 | 1 | 222 | 1 | 221 | 6.80e-156 | 431 |
| MS.gene89775.t1 | MTR_1g079490 | 79.372 | 223 | 44 | 2 | 1 | 222 | 1 | 222 | 6.96e-127 | 357 |
| MS.gene89775.t1 | MTR_6g005340 | 76.712 | 219 | 48 | 2 | 4 | 222 | 5 | 220 | 4.28e-117 | 333 |
| MS.gene89775.t1 | MTR_6g005330 | 76.233 | 223 | 49 | 3 | 1 | 222 | 1 | 220 | 6.41e-117 | 332 |
| MS.gene89775.t1 | MTR_6g005360 | 74.324 | 222 | 55 | 1 | 1 | 222 | 1 | 220 | 7.00e-117 | 332 |
| MS.gene89775.t1 | MTR_6g005310 | 76.606 | 218 | 48 | 2 | 4 | 221 | 5 | 219 | 1.66e-116 | 331 |
| MS.gene89775.t1 | MTR_6g005350 | 63.182 | 220 | 77 | 3 | 3 | 222 | 4 | 219 | 1.40e-90 | 265 |
| MS.gene89775.t1 | MTR_6g005380 | 60.766 | 209 | 81 | 1 | 1 | 209 | 1 | 208 | 8.48e-87 | 256 |
| MS.gene89775.t1 | MTR_1g023770 | 58.716 | 218 | 78 | 3 | 1 | 216 | 1 | 208 | 7.88e-80 | 238 |
| MS.gene89775.t1 | MTR_2g030855 | 59.701 | 201 | 70 | 4 | 19 | 216 | 19 | 211 | 1.34e-76 | 230 |
| MS.gene89775.t1 | MTR_2g031270 | 58.911 | 202 | 71 | 5 | 19 | 216 | 19 | 212 | 3.25e-72 | 219 |
| MS.gene89775.t1 | MTR_2g030895 | 63.218 | 174 | 57 | 4 | 47 | 216 | 7 | 177 | 4.60e-70 | 212 |
| MS.gene89775.t1 | MTR_2g030865 | 62.644 | 174 | 58 | 4 | 47 | 216 | 7 | 177 | 3.63e-68 | 207 |
| MS.gene89775.t1 | MTR_1g085500 | 46.262 | 214 | 107 | 3 | 5 | 216 | 6 | 213 | 5.69e-61 | 190 |
| MS.gene89775.t1 | MTR_1g085140 | 46.269 | 201 | 100 | 3 | 18 | 216 | 20 | 214 | 2.39e-54 | 173 |
| MS.gene89775.t1 | MTR_7g111240 | 47.716 | 197 | 95 | 3 | 22 | 216 | 20 | 210 | 8.34e-53 | 169 |
| MS.gene89775.t1 | MTR_7g111240 | 47.716 | 197 | 92 | 3 | 22 | 216 | 20 | 207 | 1.49e-51 | 166 |
| MS.gene89775.t1 | MTR_2g031300 | 53.488 | 172 | 54 | 4 | 47 | 215 | 7 | 155 | 1.72e-50 | 161 |
| MS.gene89775.t1 | MTR_5g036570 | 45.455 | 198 | 99 | 4 | 22 | 216 | 27 | 218 | 1.43e-48 | 159 |
| MS.gene89775.t1 | MTR_1g112200 | 41.500 | 200 | 102 | 5 | 19 | 216 | 20 | 206 | 3.77e-44 | 147 |
| MS.gene89775.t1 | MTR_1g060120 | 36.036 | 222 | 129 | 5 | 2 | 216 | 5 | 220 | 6.98e-41 | 139 |
| MS.gene89775.t1 | MTR_2g041550 | 40.845 | 213 | 108 | 5 | 4 | 214 | 7 | 203 | 1.79e-40 | 137 |
| MS.gene89775.t1 | MTR_2g041570 | 37.850 | 214 | 115 | 5 | 4 | 215 | 7 | 204 | 3.10e-38 | 132 |
| MS.gene89775.t1 | MTR_2g041580 | 38.028 | 213 | 114 | 5 | 4 | 214 | 7 | 203 | 3.38e-38 | 132 |
| MS.gene89775.t1 | MTR_8g007300 | 40.299 | 201 | 106 | 4 | 22 | 220 | 26 | 214 | 1.79e-36 | 127 |
| MS.gene89775.t1 | MTR_8g020750 | 40.404 | 198 | 97 | 5 | 1 | 191 | 1 | 184 | 2.92e-35 | 124 |
| MS.gene89775.t1 | MTR_8g020630 | 40.110 | 182 | 95 | 5 | 1 | 180 | 1 | 170 | 1.17e-33 | 120 |
| MS.gene89775.t1 | MTR_3g019710 | 40.500 | 200 | 104 | 6 | 1 | 198 | 1 | 187 | 1.65e-33 | 120 |
| MS.gene89775.t1 | MTR_8g020590 | 36.866 | 217 | 119 | 6 | 1 | 215 | 2 | 202 | 3.25e-33 | 119 |
| MS.gene89775.t1 | MTR_2g036120 | 37.736 | 212 | 115 | 6 | 1 | 207 | 1 | 200 | 5.24e-32 | 116 |
| MS.gene89775.t1 | MTR_8g020770 | 41.111 | 180 | 93 | 4 | 1 | 177 | 1 | 170 | 3.96e-31 | 114 |
| MS.gene89775.t1 | MTR_8g020650 | 35.321 | 218 | 125 | 6 | 1 | 216 | 1 | 204 | 4.69e-31 | 113 |
| MS.gene89775.t1 | MTR_8g020670 | 34.404 | 218 | 127 | 6 | 1 | 216 | 1 | 204 | 2.76e-30 | 111 |
| MS.gene89775.t1 | MTR_3g019650 | 38.119 | 202 | 105 | 7 | 1 | 198 | 1 | 186 | 6.13e-30 | 110 |
| MS.gene89775.t1 | MTR_3g019680 | 38.119 | 202 | 105 | 7 | 1 | 198 | 1 | 186 | 6.19e-30 | 110 |
| MS.gene89775.t1 | MTR_0284s0010 | 37.736 | 212 | 115 | 6 | 1 | 207 | 1 | 200 | 6.22e-30 | 110 |
| MS.gene89775.t1 | MTR_2g044040 | 37.778 | 180 | 98 | 5 | 1 | 178 | 1 | 168 | 1.52e-29 | 109 |
| MS.gene89775.t1 | MTR_2g086640 | 34.404 | 218 | 116 | 7 | 2 | 215 | 7 | 201 | 4.41e-29 | 108 |
| MS.gene89775.t1 | MTR_2g086620 | 43.182 | 132 | 68 | 3 | 85 | 215 | 77 | 202 | 1.36e-28 | 107 |
| MS.gene89775.t1 | MTR_2g045100 | 38.889 | 180 | 96 | 5 | 1 | 178 | 1 | 168 | 1.37e-28 | 107 |
| MS.gene89775.t1 | MTR_4g052770 | 34.862 | 218 | 114 | 7 | 3 | 215 | 9 | 203 | 1.65e-28 | 107 |
| MS.gene89775.t1 | MTR_4g052780 | 36.585 | 205 | 103 | 6 | 16 | 215 | 21 | 203 | 1.81e-28 | 107 |
| MS.gene89775.t1 | MTR_2g086630 | 42.424 | 132 | 69 | 3 | 85 | 215 | 77 | 202 | 4.56e-28 | 105 |
| MS.gene89775.t1 | MTR_3g096330 | 38.764 | 178 | 102 | 3 | 5 | 180 | 560 | 732 | 1.33e-27 | 110 |
| MS.gene89775.t1 | MTR_5g031460 | 35.071 | 211 | 122 | 5 | 1 | 207 | 1 | 200 | 1.60e-27 | 104 |
| MS.gene89775.t1 | MTR_8g020760 | 34.404 | 218 | 129 | 6 | 1 | 216 | 1 | 206 | 3.01e-26 | 101 |
| MS.gene89775.t1 | MTR_7g011340 | 35.849 | 212 | 119 | 6 | 1 | 207 | 1 | 200 | 1.89e-25 | 99.0 |
| MS.gene89775.t1 | MTR_4g010340 | 35.160 | 219 | 116 | 8 | 2 | 216 | 4 | 200 | 2.96e-25 | 98.6 |
| MS.gene89775.t1 | MTR_2g019780 | 32.727 | 220 | 131 | 7 | 1 | 216 | 1 | 207 | 1.13e-24 | 97.1 |
| MS.gene89775.t1 | MTR_8g020763 | 35.795 | 176 | 104 | 3 | 43 | 216 | 17 | 185 | 1.66e-24 | 95.9 |
| MS.gene89775.t1 | MTR_8g020610 | 33.333 | 216 | 126 | 7 | 1 | 214 | 1 | 200 | 4.01e-24 | 95.5 |
| MS.gene89775.t1 | MTR_3g073170 | 34.896 | 192 | 109 | 6 | 28 | 216 | 27 | 205 | 5.08e-24 | 95.5 |
| MS.gene89775.t1 | MTR_3g019810 | 33.981 | 206 | 113 | 9 | 1 | 203 | 107 | 292 | 1.82e-18 | 82.4 |
| MS.gene89775.t1 | MTR_2g030915 | 48.052 | 77 | 24 | 1 | 93 | 169 | 1 | 61 | 9.75e-18 | 75.9 |
| MS.gene89775.t1 | MTR_4g027415 | 35.714 | 154 | 85 | 5 | 1 | 152 | 1 | 142 | 1.12e-16 | 74.3 |
| MS.gene89775.t1 | MTR_2g072560 | 36.885 | 122 | 68 | 4 | 1 | 120 | 1 | 115 | 2.58e-13 | 65.5 |
| MS.gene89775.t1 | MTR_3g019610 | 38.393 | 112 | 60 | 4 | 1 | 110 | 1 | 105 | 4.19e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89775.t1 | AT3G05950 | 64.602 | 226 | 71 | 5 | 4 | 222 | 5 | 228 | 1.23e-94 | 276 |
| MS.gene89775.t1 | AT5G39150 | 62.557 | 219 | 78 | 3 | 1 | 216 | 1 | 218 | 3.53e-92 | 270 |
| MS.gene89775.t1 | AT5G39180 | 62.557 | 219 | 78 | 3 | 1 | 216 | 1 | 218 | 7.92e-92 | 269 |
| MS.gene89775.t1 | AT5G39120 | 63.679 | 212 | 73 | 3 | 7 | 216 | 9 | 218 | 1.12e-91 | 268 |
| MS.gene89775.t1 | AT5G39110 | 63.208 | 212 | 75 | 2 | 7 | 216 | 9 | 219 | 4.68e-90 | 264 |
| MS.gene89775.t1 | AT5G39160 | 63.182 | 220 | 75 | 4 | 1 | 216 | 1 | 218 | 4.95e-90 | 264 |
| MS.gene89775.t1 | AT5G39190 | 63.182 | 220 | 75 | 4 | 1 | 216 | 1 | 218 | 9.13e-90 | 263 |
| MS.gene89775.t1 | AT5G39130 | 63.810 | 210 | 72 | 3 | 9 | 216 | 11 | 218 | 2.78e-89 | 262 |
| MS.gene89775.t1 | AT5G39160 | 60.909 | 220 | 76 | 4 | 1 | 216 | 1 | 214 | 3.15e-85 | 252 |
| MS.gene89775.t1 | AT4G14630 | 59.545 | 220 | 84 | 3 | 1 | 216 | 3 | 221 | 4.29e-85 | 252 |
| MS.gene89775.t1 | AT3G04200 | 59.722 | 216 | 83 | 4 | 1 | 214 | 6 | 219 | 1.40e-82 | 246 |
| MS.gene89775.t1 | AT5G38910 | 59.276 | 221 | 82 | 4 | 1 | 216 | 1 | 218 | 6.59e-79 | 236 |
| MS.gene89775.t1 | AT5G39190 | 57.339 | 218 | 69 | 3 | 1 | 216 | 1 | 196 | 1.11e-77 | 232 |
| MS.gene89775.t1 | AT5G38930 | 61.307 | 199 | 73 | 2 | 20 | 216 | 24 | 220 | 1.30e-77 | 233 |
| MS.gene89775.t1 | AT5G38940 | 60.406 | 197 | 74 | 2 | 22 | 216 | 26 | 220 | 1.30e-77 | 233 |
| MS.gene89775.t1 | AT5G38940 | 60.406 | 197 | 74 | 2 | 22 | 216 | 50 | 244 | 1.93e-77 | 233 |
| MS.gene89775.t1 | AT5G39160 | 56.881 | 218 | 70 | 3 | 1 | 216 | 1 | 196 | 2.94e-77 | 231 |
| MS.gene89775.t1 | AT5G38960 | 59.406 | 202 | 77 | 3 | 18 | 216 | 22 | 221 | 4.17e-77 | 231 |
| MS.gene89775.t1 | AT3G04170 | 55.238 | 210 | 87 | 4 | 8 | 215 | 12 | 216 | 8.65e-73 | 221 |
| MS.gene89775.t1 | AT3G04150 | 53.670 | 218 | 94 | 4 | 3 | 215 | 4 | 219 | 2.75e-72 | 219 |
| MS.gene89775.t1 | AT3G04190 | 55.446 | 202 | 86 | 3 | 15 | 214 | 19 | 218 | 3.18e-72 | 219 |
| MS.gene89775.t1 | AT3G04150 | 50.870 | 230 | 95 | 4 | 3 | 215 | 4 | 232 | 1.86e-70 | 215 |
| MS.gene89775.t1 | AT3G04180 | 53.960 | 202 | 89 | 3 | 15 | 214 | 19 | 218 | 3.16e-69 | 211 |
| MS.gene89775.t1 | AT1G02335 | 43.721 | 215 | 113 | 3 | 4 | 216 | 6 | 214 | 8.45e-57 | 180 |
| MS.gene89775.t1 | AT3G62020 | 46.262 | 214 | 105 | 5 | 6 | 216 | 8 | 214 | 1.21e-55 | 177 |
| MS.gene89775.t1 | AT5G38910 | 48.416 | 221 | 69 | 4 | 1 | 216 | 1 | 181 | 2.59e-55 | 175 |
| MS.gene89775.t1 | AT3G05930 | 47.549 | 204 | 97 | 4 | 3 | 203 | 5 | 201 | 1.22e-53 | 172 |
| MS.gene89775.t1 | AT1G09560 | 45.050 | 202 | 103 | 3 | 17 | 216 | 19 | 214 | 1.66e-53 | 171 |
| MS.gene89775.t1 | AT5G26700 | 49.457 | 184 | 78 | 5 | 26 | 205 | 25 | 197 | 3.25e-51 | 165 |
| MS.gene89775.t1 | AT1G18970 | 45.274 | 201 | 100 | 4 | 18 | 216 | 25 | 217 | 6.85e-50 | 162 |
| MS.gene89775.t1 | AT1G18980 | 45.771 | 201 | 99 | 4 | 18 | 216 | 25 | 217 | 1.20e-48 | 159 |
| MS.gene89775.t1 | AT3G62020 | 46.961 | 181 | 89 | 3 | 39 | 216 | 9 | 185 | 5.28e-48 | 156 |
| MS.gene89775.t1 | AT1G10460 | 41.709 | 199 | 107 | 5 | 22 | 215 | 19 | 213 | 5.49e-45 | 149 |
| MS.gene89775.t1 | AT3G10080 | 39.409 | 203 | 113 | 5 | 18 | 216 | 22 | 218 | 1.69e-43 | 146 |
| MS.gene89775.t1 | AT3G10080 | 39.409 | 203 | 113 | 5 | 18 | 216 | 93 | 289 | 9.21e-43 | 146 |
| MS.gene89775.t1 | AT5G61750 | 41.398 | 186 | 103 | 2 | 4 | 187 | 1 | 182 | 1.98e-40 | 137 |
| MS.gene89775.t1 | AT5G20630 | 40.758 | 211 | 112 | 6 | 1 | 209 | 1 | 200 | 3.42e-40 | 137 |
| MS.gene89775.t1 | AT1G72610 | 40.314 | 191 | 104 | 6 | 26 | 214 | 19 | 201 | 7.86e-33 | 118 |
| MS.gene89775.t1 | AT1G74820 | 39.175 | 194 | 108 | 4 | 25 | 216 | 39 | 224 | 7.96e-33 | 118 |
| MS.gene89775.t1 | AT5G38950 | 54.167 | 72 | 33 | 0 | 146 | 217 | 2 | 73 | 1.30e-18 | 78.2 |
| MS.gene89775.t1 | AT5G39100 | 72.000 | 50 | 14 | 0 | 7 | 56 | 9 | 58 | 7.86e-18 | 74.7 |
Find 57 sgRNAs with CRISPR-Local
Find 129 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATATACATACCACCATCTT+TGG | 0.255130 | 4.4:-83681924 | None:intergenic |
| CCTTGTTAGCATCTTGGCTT+TGG | 0.311083 | 4.4:+83681835 | MS.gene89775:CDS |
| TTGAGAGCCTAGTGCGTTAG+AGG | 0.314204 | 4.4:-83683077 | None:intergenic |
| CTTATCCACTTGAAAAGCTT+TGG | 0.335788 | 4.4:-83683476 | None:intergenic |
| CAACACACTTGGGATATCTT+TGG | 0.340651 | 4.4:+83683135 | MS.gene89775:CDS |
| ACCATCTTTGGGGTCTTTGA+TGG | 0.343346 | 4.4:-83681912 | None:intergenic |
| TGAAAATGAATAAGACCAAT+TGG | 0.356438 | 4.4:-83683331 | None:intergenic |
| GACTGCCTTCAAGTACTATA+AGG | 0.359669 | 4.4:-83683222 | None:intergenic |
| ACGATTGTTGTTGTCTTGAT+TGG | 0.375547 | 4.4:-83683266 | None:intergenic |
| ATACTTCCTTGTTAGCATCT+TGG | 0.401215 | 4.4:+83681829 | MS.gene89775:CDS |
| TTCTTCAAACATGTTGAAGC+TGG | 0.402098 | 4.4:+83683049 | MS.gene89775:CDS |
| ATATACATACCACCATCTTT+GGG | 0.405165 | 4.4:-83681923 | None:intergenic |
| GGCAACGCTGTTGCTATTGC+TGG | 0.408860 | 4.4:+83683370 | MS.gene89775:CDS |
| AATTTGCAGTGTTTGTGAAC+GGG | 0.408992 | 4.4:+83682987 | MS.gene89775:intron |
| ATTATCTTCAAAAGCAGTCT+TGG | 0.442184 | 4.4:+83683509 | MS.gene89775:CDS |
| CAAAAGTCTTGGAGAGGGCT+AGG | 0.443544 | 4.4:-83681884 | None:intergenic |
| GTCACTGTAGACCAACTATT+CGG | 0.446134 | 4.4:+83683109 | MS.gene89775:CDS |
| GATGTGTTTGTGTTCCCAAT+TGG | 0.446317 | 4.4:+83683316 | MS.gene89775:CDS |
| TTTGCAACGGTGATAACTCC+TGG | 0.470922 | 4.4:-83683409 | None:intergenic |
| TGATGGCAACGCAAAAGTCT+TGG | 0.475943 | 4.4:-83681895 | None:intergenic |
| ATAAGGATTTCCGTTCCTCT+AGG | 0.478305 | 4.4:-83683205 | None:intergenic |
| GAAATCCTTATAGTACTTGA+AGG | 0.481512 | 4.4:+83683217 | MS.gene89775:CDS |
| TCTTCAGCTTTAACTAGCGC+GGG | 0.484814 | 4.4:-83683025 | None:intergenic |
| CTTGAAGGCAGTCTTTATGT+TGG | 0.489113 | 4.4:+83683232 | MS.gene89775:CDS |
| CTCTAGGGTGAATGTGAGGT+GGG | 0.498933 | 4.4:-83683189 | None:intergenic |
| TCTTCAAACATGTTGAAGCT+GGG | 0.501315 | 4.4:+83683050 | MS.gene89775:CDS |
| GAAAATGAATAAGACCAATT+GGG | 0.510497 | 4.4:-83683330 | None:intergenic |
| AGAACTTCGTCGGAAATAAG+AGG | 0.511108 | 4.4:-83683451 | None:intergenic |
| CTATTCGGACTCAACACACT+TGG | 0.521532 | 4.4:+83683124 | MS.gene89775:CDS |
| TGAGGTGGGTTTAAACCCTT+AGG | 0.539104 | 4.4:-83683175 | None:intergenic |
| TTTCAACTGAATGTGGGCTA+TGG | 0.539971 | 4.4:+83683349 | MS.gene89775:CDS |
| GTGTGTTGAGTCCGAATAGT+TGG | 0.540274 | 4.4:-83683120 | None:intergenic |
| ATCTTCAGCTTTAACTAGCG+CGG | 0.543736 | 4.4:-83683026 | None:intergenic |
| TAAGGATTTCCGTTCCTCTA+GGG | 0.546102 | 4.4:-83683204 | None:intergenic |
| TAATTTGCAGTGTTTGTGAA+CGG | 0.551349 | 4.4:+83682986 | MS.gene89775:intron |
| GCCATCAAAGACCCCAAAGA+TGG | 0.552100 | 4.4:+83681911 | MS.gene89775:CDS |
| AGCTTTGGTAAGAACTTCGT+CGG | 0.555884 | 4.4:-83683461 | None:intergenic |
| CCAAAGCCAAGATGCTAACA+AGG | 0.556137 | 4.4:-83681835 | None:intergenic |
| CCTCTAGGGTGAATGTGAGG+TGG | 0.565620 | 4.4:-83683190 | None:intergenic |
| AAAAGTCTTGGAGAGGGCTA+GGG | 0.571621 | 4.4:-83681883 | None:intergenic |
| GGGAATGCCTCTAACGCACT+AGG | 0.573570 | 4.4:+83683070 | MS.gene89775:CDS |
| GGACTTAGCAGTCAAAATCC+AGG | 0.578792 | 4.4:+83683391 | MS.gene89775:CDS |
| TATTCGGACTCAACACACTT+GGG | 0.584132 | 4.4:+83683125 | MS.gene89775:CDS |
| TTTAAACAAAGCATTTGCAA+CGG | 0.590900 | 4.4:-83683422 | None:intergenic |
| TTCACCAAAGTTCTCAACAA+GGG | 0.595598 | 4.4:+83683292 | MS.gene89775:CDS |
| CTTCACCAAAGTTCTCAACA+AGG | 0.598137 | 4.4:+83683291 | MS.gene89775:CDS |
| TCACCAAAGTTCTCAACAAG+GGG | 0.602098 | 4.4:+83683293 | MS.gene89775:CDS |
| AGGGCTAGGGTCATAAGCAA+AGG | 0.622370 | 4.4:-83681870 | None:intergenic |
| TCACATTCACCCTAGAGGAA+CGG | 0.635178 | 4.4:+83683195 | MS.gene89775:CDS |
| ATCAAAGACCCCAAAGATGG+TGG | 0.635231 | 4.4:+83681914 | MS.gene89775:CDS |
| TATACATACCACCATCTTTG+GGG | 0.639620 | 4.4:-83681922 | None:intergenic |
| CAACGCAAAAGTCTTGGAGA+GGG | 0.643972 | 4.4:-83681889 | None:intergenic |
| GCAACGCAAAAGTCTTGGAG+AGG | 0.646350 | 4.4:-83681890 | None:intergenic |
| CTTCAGCTTTAACTAGCGCG+GGG | 0.662073 | 4.4:-83683024 | None:intergenic |
| GTTCCTCTAGGGTGAATGTG+AGG | 0.681575 | 4.4:-83683193 | None:intergenic |
| CCACCTCACATTCACCCTAG+AGG | 0.685266 | 4.4:+83683190 | MS.gene89775:CDS |
| CACCAAAGTTCTCAACAAGG+GGG | 0.737020 | 4.4:+83683294 | MS.gene89775:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAAATACATATTGT+AGG | - | chr4.4:83682748-83682767 | None:intergenic | 10.0% |
| !! | ATATTTAGTATTATAGTATA+TGG | + | chr4.4:83682779-83682798 | MS.gene89775:intron | 10.0% |
| !! | ATTAAAATGATGATTAAATT+TGG | - | chr4.4:83682291-83682310 | None:intergenic | 10.0% |
| !!! | TAATTTTTTACTAAATAATG+GGG | - | chr4.4:83682139-83682158 | None:intergenic | 10.0% |
| !! | ACCTTATAGTAATATATAAA+TGG | - | chr4.4:83682705-83682724 | None:intergenic | 15.0% |
| !! | ATACTAAAATTAATAAGTAG+TGG | - | chr4.4:83682087-83682106 | None:intergenic | 15.0% |
| !! | ATTATTTGTATGTACTTAAT+AGG | - | chr4.4:83682551-83682570 | None:intergenic | 15.0% |
| !! | TAAATGAAATATGAATTAGT+TGG | - | chr4.4:83682192-83682211 | None:intergenic | 15.0% |
| !! | TCCATTTATATATTACTATA+AGG | + | chr4.4:83682701-83682720 | MS.gene89775:intron | 15.0% |
| !!! | AAATATAGAATCTTTTGTTT+TGG | + | chr4.4:83682884-83682903 | MS.gene89775:intron | 15.0% |
| !!! | AATTTTTTACTAAATAATGG+GGG | - | chr4.4:83682138-83682157 | None:intergenic | 15.0% |
| !!! | TTATTTTACTATAGTAAAGT+TGG | + | chr4.4:83682165-83682184 | MS.gene89775:intron | 15.0% |
| !! | AAAGAAAATTTATGTAGAGT+CGG | - | chr4.4:83682842-83682861 | None:intergenic | 20.0% |
| !! | CCTATTTAACTAAAAAAGTT+AGG | + | chr4.4:83682484-83682503 | MS.gene89775:intron | 20.0% |
| !! | CCTGTTAAACAAAAAAAAAT+GGG | - | chr4.4:83682442-83682461 | None:intergenic | 20.0% |
| !! | TAATCATGACTTAAAAGTTA+AGG | - | chr4.4:83681952-83681971 | None:intergenic | 20.0% |
| !!! | AATAGGCTTCTAAAAAATAT+TGG | - | chr4.4:83682470-83682489 | None:intergenic | 20.0% |
| !!! | CCATTTTTTTTTGTTTAACA+GGG | + | chr4.4:83682440-83682459 | MS.gene89775:intron | 20.0% |
| !!! | CCTAACTTTTTTAGTTAAAT+AGG | - | chr4.4:83682487-83682506 | None:intergenic | 20.0% |
| ! | ACATTTCAATAAATACCAAG+TGG | - | chr4.4:83682631-83682650 | None:intergenic | 25.0% |
| ! | CAAAAAGTCTAATATTTGCA+GGG | - | chr4.4:83682013-83682032 | None:intergenic | 25.0% |
| ! | CCCTGTTAAACAAAAAAAAA+TGG | - | chr4.4:83682443-83682462 | None:intergenic | 25.0% |
| ! | CTAAAATTAATAAGTAGTGG+AGG | - | chr4.4:83682084-83682103 | None:intergenic | 25.0% |
| ! | GAAAATGAATAAGACCAATT+GGG | - | chr4.4:83683333-83683352 | None:intergenic | 25.0% |
| ! | TGAAAATGAATAAGACCAAT+TGG | - | chr4.4:83683334-83683353 | None:intergenic | 25.0% |
| !! | AACCATAGATAAAGTCTTTT+AGG | + | chr4.4:83682512-83682531 | MS.gene89775:intron | 25.0% |
| !! | ATTCATTTTCAACTGAATGT+GGG | + | chr4.4:83683343-83683362 | MS.gene89775:CDS | 25.0% |
| !! | TATTCATTTTCAACTGAATG+TGG | + | chr4.4:83683342-83683361 | MS.gene89775:CDS | 25.0% |
| !! | TTTAAACAAAGCATTTGCAA+CGG | - | chr4.4:83683425-83683444 | None:intergenic | 25.0% |
| !!! | CCCATTTTTTTTTGTTTAAC+AGG | + | chr4.4:83682439-83682458 | MS.gene89775:intron | 25.0% |
| AAGACTTTATCTATGGTTTG+TGG | - | chr4.4:83682510-83682529 | None:intergenic | 30.0% | |
| ATATACATACCACCATCTTT+GGG | - | chr4.4:83681926-83681945 | None:intergenic | 30.0% | |
| ATTATCTTCAAAAGCAGTCT+TGG | + | chr4.4:83683509-83683528 | MS.gene89775:CDS | 30.0% | |
| CCAAAAAGTCTAATATTTGC+AGG | - | chr4.4:83682014-83682033 | None:intergenic | 30.0% | |
| GAAATCCTTATAGTACTTGA+AGG | + | chr4.4:83683217-83683236 | MS.gene89775:CDS | 30.0% | |
| TATATACATACCACCATCTT+TGG | - | chr4.4:83681927-83681946 | None:intergenic | 30.0% | |
| ! | AGCCAGAAATTTTAGATTGT+GGG | - | chr4.4:83682813-83682832 | None:intergenic | 30.0% |
| ! | CCTGCAAATATTAGACTTTT+TGG | + | chr4.4:83682011-83682030 | MS.gene89775:intron | 30.0% |
| ! | TAATTTGCAGTGTTTGTGAA+CGG | + | chr4.4:83682986-83683005 | MS.gene89775:intron | 30.0% |
| !!! | GAATCTTTTGTTTTGGTTCA+CGG | + | chr4.4:83682891-83682910 | MS.gene89775:intron | 30.0% |
| AATTTGCAGTGTTTGTGAAC+GGG | + | chr4.4:83682987-83683006 | MS.gene89775:intron | 35.0% | |
| ATACTTCCTTGTTAGCATCT+TGG | + | chr4.4:83681829-83681848 | MS.gene89775:CDS | 35.0% | |
| ATGTAAGCTAGGTTAAAGGT+TGG | - | chr4.4:83682411-83682430 | None:intergenic | 35.0% | |
| CCTAATGTAAGCTAGGTTAA+AGG | - | chr4.4:83682415-83682434 | None:intergenic | 35.0% | |
| CCTTTAACCTAGCTTACATT+AGG | + | chr4.4:83682412-83682431 | MS.gene89775:intron | 35.0% | |
| CTTATCCACTTGAAAAGCTT+TGG | - | chr4.4:83683479-83683498 | None:intergenic | 35.0% | |
| GAAAATTTATGTAGAGTCGG+TGG | - | chr4.4:83682839-83682858 | None:intergenic | 35.0% | |
| GGCCTAAAAGACTTTATCTA+TGG | - | chr4.4:83682517-83682536 | None:intergenic | 35.0% | |
| TATACATACCACCATCTTTG+GGG | - | chr4.4:83681925-83681944 | None:intergenic | 35.0% | |
| TCTTCAAACATGTTGAAGCT+GGG | + | chr4.4:83683050-83683069 | MS.gene89775:CDS | 35.0% | |
| TGTAAGCTAGGTTAAAGGTT+GGG | - | chr4.4:83682410-83682429 | None:intergenic | 35.0% | |
| TTCACCAAAGTTCTCAACAA+GGG | + | chr4.4:83683292-83683311 | MS.gene89775:CDS | 35.0% | |
| TTCTTCAAACATGTTGAAGC+TGG | + | chr4.4:83683049-83683068 | MS.gene89775:CDS | 35.0% | |
| ! | AAATAATGGGGGCATAAAGT+TGG | - | chr4.4:83682127-83682146 | None:intergenic | 35.0% |
| ! | ACGATTGTTGTTGTCTTGAT+TGG | - | chr4.4:83683269-83683288 | None:intergenic | 35.0% |
| ! | CCAGAAATTTTAGATTGTGG+GGG | - | chr4.4:83682811-83682830 | None:intergenic | 35.0% |
| ! | GAGCCAGAAATTTTAGATTG+TGG | - | chr4.4:83682814-83682833 | None:intergenic | 35.0% |
| ! | GCCAGAAATTTTAGATTGTG+GGG | - | chr4.4:83682812-83682831 | None:intergenic | 35.0% |
| ! | TAAAGTCTTTTAGGCCAACA+AGG | + | chr4.4:83682521-83682540 | MS.gene89775:intron | 35.0% |
| !! | TCTTACCAAAGCTTTTCAAG+TGG | + | chr4.4:83683471-83683490 | MS.gene89775:CDS | 35.0% |
| !!! | ATTTTAGCAGTTTCTAGCCA+TGG | - | chr4.4:83682937-83682956 | None:intergenic | 35.0% |
| !!! | CTTTTTGGTTTTACTCCATG+TGG | + | chr4.4:83682026-83682045 | MS.gene89775:intron | 35.0% |
| AATTTATGTAGAGTCGGTGG+CGG | - | chr4.4:83682836-83682855 | None:intergenic | 40.0% | |
| ACTTAATAGGCCAACCTTGT+TGG | - | chr4.4:83682538-83682557 | None:intergenic | 40.0% | |
| AGAACTTCGTCGGAAATAAG+AGG | - | chr4.4:83683454-83683473 | None:intergenic | 40.0% | |
| ATAAGGATTTCCGTTCCTCT+AGG | - | chr4.4:83683208-83683227 | None:intergenic | 40.0% | |
| CAACACACTTGGGATATCTT+TGG | + | chr4.4:83683135-83683154 | MS.gene89775:CDS | 40.0% | |
| CTTCACCAAAGTTCTCAACA+AGG | + | chr4.4:83683291-83683310 | MS.gene89775:CDS | 40.0% | |
| CTTGAAGGCAGTCTTTATGT+TGG | + | chr4.4:83683232-83683251 | MS.gene89775:CDS | 40.0% | |
| GACTGCCTTCAAGTACTATA+AGG | - | chr4.4:83683225-83683244 | None:intergenic | 40.0% | |
| GATGTGTTTGTGTTCCCAAT+TGG | + | chr4.4:83683316-83683335 | MS.gene89775:CDS | 40.0% | |
| GTCACTGTAGACCAACTATT+CGG | + | chr4.4:83683109-83683128 | MS.gene89775:CDS | 40.0% | |
| TAAGGATTTCCGTTCCTCTA+GGG | - | chr4.4:83683207-83683226 | None:intergenic | 40.0% | |
| TATTCGGACTCAACACACTT+GGG | + | chr4.4:83683125-83683144 | MS.gene89775:CDS | 40.0% | |
| TCACCAAAGTTCTCAACAAG+GGG | + | chr4.4:83683293-83683312 | MS.gene89775:CDS | 40.0% | |
| ! | AGCTTTGGTAAGAACTTCGT+CGG | - | chr4.4:83683464-83683483 | None:intergenic | 40.0% |
| ! | ATCTTCAGCTTTAACTAGCG+CGG | - | chr4.4:83683029-83683048 | None:intergenic | 40.0% |
| ! | CCCCCACAATCTAAAATTTC+TGG | + | chr4.4:83682808-83682827 | MS.gene89775:intron | 40.0% |
| ! | TTTCAACTGAATGTGGGCTA+TGG | + | chr4.4:83683349-83683368 | MS.gene89775:CDS | 40.0% |
| !! | GAAGTCACTGACTTGAGAAT+TGG | - | chr4.4:83682609-83682628 | None:intergenic | 40.0% |
| !!! | TTTGGTTTTACTCCATGTGG+CGG | + | chr4.4:83682029-83682048 | MS.gene89775:intron | 40.0% |
| AAAAGTCTTGGAGAGGGCTA+GGG | - | chr4.4:83681886-83681905 | None:intergenic | 45.0% | |
| AGCTACATGTACCCTTGTGT+GGG | + | chr4.4:83682052-83682071 | MS.gene89775:intron | 45.0% | |
| ATCAAAGACCCCAAAGATGG+TGG | + | chr4.4:83681914-83681933 | MS.gene89775:CDS | 45.0% | |
| ATCCCCCTTGTTGAGAACTT+TGG | - | chr4.4:83683299-83683318 | None:intergenic | 45.0% | |
| CAACGCAAAAGTCTTGGAGA+GGG | - | chr4.4:83681892-83681911 | None:intergenic | 45.0% | |
| CACCAAAGTTCTCAACAAGG+GGG | + | chr4.4:83683294-83683313 | MS.gene89775:CDS | 45.0% | |
| CCAAAGCCAAGATGCTAACA+AGG | - | chr4.4:83681838-83681857 | None:intergenic | 45.0% | |
| CCTTGTTAGCATCTTGGCTT+TGG | + | chr4.4:83681835-83681854 | MS.gene89775:CDS | 45.0% | |
| CTATTCGGACTCAACACACT+TGG | + | chr4.4:83683124-83683143 | MS.gene89775:CDS | 45.0% | |
| GGAATAGGCTGAGTCAAGTT+AGG | - | chr4.4:83682582-83682601 | None:intergenic | 45.0% | |
| GGACTTAGCAGTCAAAATCC+AGG | + | chr4.4:83683391-83683410 | MS.gene89775:CDS | 45.0% | |
| GTGTGTTGAGTCCGAATAGT+TGG | - | chr4.4:83683123-83683142 | None:intergenic | 45.0% | |
| TCAAGTCAGTGACTTCCACT+TGG | + | chr4.4:83682613-83682632 | MS.gene89775:intron | 45.0% | |
| TCACATTCACCCTAGAGGAA+CGG | + | chr4.4:83683195-83683214 | MS.gene89775:CDS | 45.0% | |
| TGATGGCAACGCAAAAGTCT+TGG | - | chr4.4:83681898-83681917 | None:intergenic | 45.0% | |
| TTGAGAATTGGAGCGGGAAT+AGG | - | chr4.4:83682597-83682616 | None:intergenic | 45.0% | |
| TTTGCAACGGTGATAACTCC+TGG | - | chr4.4:83683412-83683431 | None:intergenic | 45.0% | |
| ! | GTCTTTTAGGCCAACAAGGT+TGG | + | chr4.4:83682525-83682544 | MS.gene89775:intron | 45.0% |
| ! | TCTTCAGCTTTAACTAGCGC+GGG | - | chr4.4:83683028-83683047 | None:intergenic | 45.0% |
| ! | TGAGGTGGGTTTAAACCCTT+AGG | - | chr4.4:83683178-83683197 | None:intergenic | 45.0% |
| !! | ACCATCTTTGGGGTCTTTGA+TGG | - | chr4.4:83681915-83681934 | None:intergenic | 45.0% |
| !! | ACTGACTTGAGAATTGGAGC+GGG | - | chr4.4:83682603-83682622 | None:intergenic | 45.0% |
| !! | CACTGACTTGAGAATTGGAG+CGG | - | chr4.4:83682604-83682623 | None:intergenic | 45.0% |
| !!! | TTTTGGTTCACGGTGTTTCC+TGG | + | chr4.4:83682901-83682920 | MS.gene89775:intron | 45.0% |
| !!! | TTAATTTTTTACTAAATAAT+GGG | - | chr4.4:83682140-83682159 | None:intergenic | 5.0% |
| !!! | TTTAATTTTTTACTAAATAA+TGG | - | chr4.4:83682141-83682160 | None:intergenic | 5.0% |
| AGGGCTAGGGTCATAAGCAA+AGG | - | chr4.4:83681873-83681892 | None:intergenic | 50.0% | |
| CAAAAGTCTTGGAGAGGGCT+AGG | - | chr4.4:83681887-83681906 | None:intergenic | 50.0% | |
| CGGTGTTTCCTGGTTTAGCT+AGG | + | chr4.4:83682911-83682930 | MS.gene89775:intron | 50.0% | |
| GAGCTACATGTACCCTTGTG+TGG | + | chr4.4:83682051-83682070 | MS.gene89775:intron | 50.0% | |
| GCAACGCAAAAGTCTTGGAG+AGG | - | chr4.4:83681893-83681912 | None:intergenic | 50.0% | |
| GCCATCAAAGACCCCAAAGA+TGG | + | chr4.4:83681911-83681930 | MS.gene89775:CDS | 50.0% | |
| GGGCTCACCTAATGTAAGCT+AGG | - | chr4.4:83682422-83682441 | None:intergenic | 50.0% | |
| TTCCTGGTTTAGCTAGGCCA+TGG | + | chr4.4:83682917-83682936 | MS.gene89775:intron | 50.0% | |
| TTGAGAGCCTAGTGCGTTAG+AGG | - | chr4.4:83683080-83683099 | None:intergenic | 50.0% | |
| ! | CTTCAGCTTTAACTAGCGCG+GGG | - | chr4.4:83683027-83683046 | None:intergenic | 50.0% |
| !! | CTCTAGGGTGAATGTGAGGT+GGG | - | chr4.4:83683192-83683211 | None:intergenic | 50.0% |
| !! | GTTCCTCTAGGGTGAATGTG+AGG | - | chr4.4:83683196-83683215 | None:intergenic | 50.0% |
| !!! | CGCGTTGATTTTGCGCCTAA+GGG | + | chr4.4:83683160-83683179 | MS.gene89775:CDS | 50.0% |
| !!! | TCGCGTTGATTTTGCGCCTA+AGG | + | chr4.4:83683159-83683178 | MS.gene89775:CDS | 50.0% |
| AGCCATGGCCTAGCTAAACC+AGG | - | chr4.4:83682922-83682941 | None:intergenic | 55.0% | |
| CCACCTCACATTCACCCTAG+AGG | + | chr4.4:83683190-83683209 | MS.gene89775:CDS | 55.0% | |
| GGGAATGCCTCTAACGCACT+AGG | + | chr4.4:83683070-83683089 | MS.gene89775:CDS | 55.0% | |
| GTACATGTAGCTCCGCCACA+TGG | - | chr4.4:83682044-83682063 | None:intergenic | 55.0% | |
| ! | GGCAACGCTGTTGCTATTGC+TGG | + | chr4.4:83683370-83683389 | MS.gene89775:CDS | 55.0% |
| !! | CCTCTAGGGTGAATGTGAGG+TGG | - | chr4.4:83683193-83683212 | None:intergenic | 55.0% |
| ! | GGAGGCACATGCCCACACAA+GGG | - | chr4.4:83682066-83682085 | None:intergenic | 60.0% |
| ! | TGGAGGCACATGCCCACACA+AGG | - | chr4.4:83682067-83682086 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 83681818 | 83683546 | 83681818 | ID=MS.gene89775 |
| chr4.4 | mRNA | 83681818 | 83683546 | 83681818 | ID=MS.gene89775.t1;Parent=MS.gene89775 |
| chr4.4 | exon | 83681818 | 83681935 | 83681818 | ID=MS.gene89775.t1.exon1;Parent=MS.gene89775.t1 |
| chr4.4 | CDS | 83681818 | 83681935 | 83681818 | ID=cds.MS.gene89775.t1;Parent=MS.gene89775.t1 |
| chr4.4 | exon | 83682996 | 83683546 | 83682996 | ID=MS.gene89775.t1.exon2;Parent=MS.gene89775.t1 |
| chr4.4 | CDS | 83682996 | 83683546 | 83682996 | ID=cds.MS.gene89775.t1;Parent=MS.gene89775.t1 |
| Gene Sequence |
| Protein sequence |
