Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99705.t1 | AES98921.1 | 92.2 | 167 | 12 | 1 | 3 | 168 | 1 | 167 | 9.30E-82 | 313.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99705.t1 | Q8GWL2 | 47.1 | 153 | 68 | 3 | 16 | 168 | 16 | 155 | 4.3e-27 | 123.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99705.t1 | G7KFJ0 | 92.2 | 167 | 12 | 1 | 3 | 168 | 1 | 167 | 6.7e-82 | 313.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene99705 | MS.gene87762 | PPI |
| MS.gene99705 | MS.gene42091 | PPI |
| MS.gene99705 | MS.gene42103 | PPI |
| MS.gene99705 | MS.gene87765 | PPI |
| MS.gene99705 | MS.gene87038 | PPI |
| MS.gene99705 | MS.gene42096 | PPI |
| MS.gene42103 | MS.gene99705 | PPI |
| MS.gene99705 | MS.gene87041 | PPI |
| MS.gene42098 | MS.gene99705 | PPI |
| MS.gene99705 | MS.gene42848 | PPI |
| MS.gene99705 | MS.gene87039 | PPI |
| MS.gene99705 | MS.gene42099 | PPI |
| MS.gene87043 | MS.gene99705 | PPI |
| MS.gene99705 | MS.gene87046 | PPI |
| MS.gene87767 | MS.gene99705 | PPI |
| MS.gene99705 | MS.gene87040 | PPI |
| MS.gene99705 | MS.gene87769 | PPI |
| MS.gene99705 | MS.gene87768 | PPI |
| MS.gene99705 | MS.gene42100 | PPI |
| MS.gene99705 | MS.gene42097 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99705.t1 | MTR_5g074550 | 92.216 | 167 | 12 | 1 | 3 | 168 | 1 | 167 | 1.42e-111 | 320 |
| MS.gene99705.t1 | MTR_5g074560 | 75.148 | 169 | 32 | 2 | 1 | 168 | 1 | 160 | 7.99e-85 | 251 |
| MS.gene99705.t1 | MTR_7g072170 | 32.624 | 141 | 83 | 4 | 19 | 154 | 33 | 166 | 2.44e-15 | 73.6 |
| MS.gene99705.t1 | MTR_5g074510 | 69.231 | 52 | 13 | 1 | 208 | 259 | 175 | 223 | 1.19e-14 | 74.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99705.t1 | AT2G30270 | 41.830 | 153 | 76 | 2 | 16 | 168 | 16 | 155 | 3.07e-29 | 109 |
Find 60 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGAACTCCGGCGTGTATTT+TGG | 0.154026 | 5.2:+62404655 | None:intergenic |
| GCTTTCTCAGACAAACTTTC+TGG | 0.199557 | 5.2:+62397761 | None:intergenic |
| ACTTGTGACTTAAAAGTAAT+TGG | 0.201896 | 5.2:-62403510 | MS.gene99705:CDS |
| TACCCCGACTGACATATATT+TGG | 0.239979 | 5.2:+62401924 | None:intergenic |
| CTTTGGAGACGAAGAGGAAT+TGG | 0.284571 | 5.2:+62404562 | None:intergenic |
| ATTATATTAGGCAGTCTTTC+CGG | 0.287199 | 5.2:-62397864 | MS.gene99705:intron |
| TGGGACTTGGAAATGTTATA+AGG | 0.293104 | 5.2:-62403643 | MS.gene99705:CDS |
| GGGAAGTGGTGATGAGAATA+AGG | 0.296657 | 5.2:-62403622 | MS.gene99705:CDS |
| AACTTTCTGGCGTGAGCTTT+AGG | 0.311592 | 5.2:+62397774 | None:intergenic |
| GGAAATGGAAACTTCAGATT+TGG | 0.316314 | 5.2:-62404726 | MS.gene99705:CDS |
| AGCAAGAGTTTGGTATCCTT+TGG | 0.337774 | 5.2:+62404545 | None:intergenic |
| TATTACTAGAGTTGAGTTAG+AGG | 0.362181 | 5.2:-62403565 | MS.gene99705:CDS |
| ATTGGAATCAAGCAAGAGTT+TGG | 0.382566 | 5.2:+62404535 | None:intergenic |
| CATTTCTAAAGACGGCGATT+TGG | 0.386460 | 5.2:-62403463 | MS.gene99705:CDS |
| TTTGGTTAAGCTTGTACATC+AGG | 0.391771 | 5.2:+62401942 | None:intergenic |
| AGACGAAGAGGAATTGGGAT+TGG | 0.410407 | 5.2:+62404568 | None:intergenic |
| ATCGGAAATGATAAACTTGC+CGG | 0.410801 | 5.2:-62401306 | MS.gene99705:CDS |
| CGATAGACTTAATTATTTGT+CGG | 0.424133 | 5.2:+62401325 | None:intergenic |
| GACGAAGAGGAATTGGGATT+GGG | 0.428563 | 5.2:+62404569 | None:intergenic |
| TTTGGAGACGAAGAGGAATT+GGG | 0.442315 | 5.2:+62404563 | None:intergenic |
| AATTGGGATTGGGTGGTTGA+CGG | 0.447331 | 5.2:+62404579 | None:intergenic |
| ATGAAAGGATTTCAAAATAG+TGG | 0.454866 | 5.2:-62397604 | None:intergenic |
| TTAGCGTTTGCTGATGCATC+GGG | 0.457884 | 5.2:-62404623 | MS.gene99705:CDS |
| GAAGAACAGAGGATTATCAT+TGG | 0.463772 | 5.2:+62404517 | None:intergenic |
| GGGACTTGGAAATGTTATAA+GGG | 0.475852 | 5.2:-62403642 | MS.gene99705:CDS |
| TTCTAAAGACGGCGATTTGG+TGG | 0.477337 | 5.2:-62403460 | MS.gene99705:CDS |
| TAACCAAATATATGTCAGTC+GGG | 0.478079 | 5.2:-62401927 | MS.gene99705:CDS |
| TCAAGTCTTTCTTCCTGTGC+CGG | 0.482471 | 5.2:+62397845 | None:intergenic |
| GATTGGGATGTGTTGATCTT+CGG | 0.483082 | 5.2:+62404691 | None:intergenic |
| ATCGGGTAACATAGTCTTCA+AGG | 0.483954 | 5.2:-62404606 | MS.gene99705:CDS |
| TGGAACCGAAAAGATCGATT+GGG | 0.488185 | 5.2:+62404675 | None:intergenic |
| TTGGAACCGAAAAGATCGAT+TGG | 0.491235 | 5.2:+62404674 | None:intergenic |
| TTAGGCAGTCTTTCCGGCAC+AGG | 0.495417 | 5.2:-62397858 | MS.gene99705:intron |
| CTTAGCGTTTGCTGATGCAT+CGG | 0.528232 | 5.2:-62404624 | MS.gene99705:CDS |
| TGGAAATGTTATAAGGGAAG+TGG | 0.531416 | 5.2:-62403636 | MS.gene99705:CDS |
| TTAACCAAATATATGTCAGT+CGG | 0.545747 | 5.2:-62401928 | MS.gene99705:CDS |
| CGACAAATAATTAAGTCTAT+CGG | 0.549349 | 5.2:-62401324 | MS.gene99705:CDS |
| ATGCTGCATGATCTCTTGAA+AGG | 0.549398 | 5.2:+62403483 | None:intergenic |
| CAATTCCTCTTCGTCTCCAA+AGG | 0.553127 | 5.2:-62404561 | MS.gene99705:CDS |
| GAAGAGGAATTGGGATTGGG+TGG | 0.563577 | 5.2:+62404572 | None:intergenic |
| TAAGATTCCACGTGGAACTC+CGG | 0.563839 | 5.2:+62404643 | None:intergenic |
| TACTCCACACTCAAAACCGC+CGG | 0.567099 | 5.2:+62401287 | None:intergenic |
| AGACGGCGATTTGGTGGCAC+AGG | 0.568219 | 5.2:-62403454 | MS.gene99705:intron |
| AGCTTATGTTAGAAGTGAAG+AGG | 0.583963 | 5.2:-62403599 | MS.gene99705:CDS |
| GTTCTGTCAGCTGTGTCTGT+TGG | 0.595569 | 5.2:-62401414 | MS.gene99705:intron |
| TATACCTGCTGGTACATCCA+TGG | 0.604416 | 5.2:+62397733 | None:intergenic |
| GTCTGAGAAAGCTGTAGCCA+TGG | 0.605522 | 5.2:-62397750 | MS.gene99705:CDS |
| GTAGCCATGGATGTACCAGC+AGG | 0.607119 | 5.2:-62397737 | MS.gene99705:intron |
| GGTATCCTTTGGAGACGAAG+AGG | 0.609702 | 5.2:+62404556 | None:intergenic |
| TCATGCAGCATTTCTAAAGA+CGG | 0.613787 | 5.2:-62403471 | MS.gene99705:CDS |
| AAGAGAGACTAACGTGATGA+CGG | 0.614624 | 5.2:+62404489 | None:intergenic |
| ATTCTAGCAAACTTTCAACC+AGG | 0.616573 | 5.2:+62401352 | None:intergenic |
| ACTTCAGATTTGGAGTACAC+AGG | 0.646841 | 5.2:-62404716 | MS.gene99705:CDS |
| AACCAAATATATGTCAGTCG+GGG | 0.653307 | 5.2:-62401926 | MS.gene99705:intron |
| TTCGGTTCCAAAATACACGC+CGG | 0.660070 | 5.2:-62404662 | MS.gene99705:CDS |
| AGAGACTAACGTGATGACGG+TGG | 0.688775 | 5.2:+62404492 | None:intergenic |
| GGAAATGATAAACTTGCCGG+CGG | 0.694121 | 5.2:-62401303 | MS.gene99705:CDS |
| ATACACGCCGGAGTTCCACG+TGG | 0.714642 | 5.2:-62404650 | MS.gene99705:CDS |
| TGACGGTGGATGAAGAACAG+AGG | 0.729871 | 5.2:+62404506 | None:intergenic |
| GCAAACGCTAAGATTCCACG+TGG | 0.780342 | 5.2:+62404635 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 62397607 | 62404750 | 62397607 | ID=MS.gene99705 |
| chr5.2 | mRNA | 62397607 | 62404750 | 62397607 | ID=MS.gene99705.t1;Parent=MS.gene99705 |
| chr5.2 | exon | 62404502 | 62404750 | 62404502 | ID=MS.gene99705.t1.exon1;Parent=MS.gene99705.t1 |
| chr5.2 | CDS | 62404502 | 62404750 | 62404502 | ID=cds.MS.gene99705.t1;Parent=MS.gene99705.t1 |
| chr5.2 | exon | 62403455 | 62403667 | 62403455 | ID=MS.gene99705.t1.exon2;Parent=MS.gene99705.t1 |
| chr5.2 | CDS | 62403455 | 62403667 | 62403455 | ID=cds.MS.gene99705.t1;Parent=MS.gene99705.t1 |
| chr5.2 | exon | 62401927 | 62401969 | 62401927 | ID=MS.gene99705.t1.exon3;Parent=MS.gene99705.t1 |
| chr5.2 | CDS | 62401927 | 62401969 | 62401927 | ID=cds.MS.gene99705.t1;Parent=MS.gene99705.t1 |
| chr5.2 | exon | 62401415 | 62401435 | 62401415 | ID=MS.gene99705.t1.exon4;Parent=MS.gene99705.t1 |
| chr5.2 | CDS | 62401415 | 62401435 | 62401415 | ID=cds.MS.gene99705.t1;Parent=MS.gene99705.t1 |
| chr5.2 | exon | 62401281 | 62401375 | 62401281 | ID=MS.gene99705.t1.exon5;Parent=MS.gene99705.t1 |
| chr5.2 | CDS | 62401281 | 62401375 | 62401281 | ID=cds.MS.gene99705.t1;Parent=MS.gene99705.t1 |
| chr5.2 | exon | 62397738 | 62397876 | 62397738 | ID=MS.gene99705.t1.exon6;Parent=MS.gene99705.t1 |
| chr5.2 | CDS | 62397738 | 62397876 | 62397738 | ID=cds.MS.gene99705.t1;Parent=MS.gene99705.t1 |
| chr5.2 | exon | 62397607 | 62397653 | 62397607 | ID=MS.gene99705.t1.exon7;Parent=MS.gene99705.t1 |
| chr5.2 | CDS | 62397607 | 62397653 | 62397607 | ID=cds.MS.gene99705.t1;Parent=MS.gene99705.t1 |
| Gene Sequence |
| Protein sequence |
