AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87039


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87039.t1 MTR_7g072120 78.571 210 37 4 1 202 1 210 3.32e-119 337
MS.gene87039.t1 MTR_7g072270 76.329 207 43 1 1 201 1 207 1.22e-117 333
MS.gene87039.t1 MTR_7g072170 75.238 210 44 2 1 202 1 210 2.61e-114 325
MS.gene87039.t1 MTR_7g072140 74.286 210 46 2 1 202 1 210 3.70e-113 322
MS.gene87039.t1 MTR_7g072250 68.627 204 50 2 7 200 38 237 3.68e-97 282
MS.gene87039.t1 MTR_7g072270 75.758 165 34 1 1 159 1 165 4.12e-91 264
MS.gene87039.t1 MTR_7g071710 94.017 117 7 0 86 202 1 117 8.86e-79 231
MS.gene87039.t1 MTR_4g029430 57.219 187 75 2 16 201 15 197 1.27e-75 226
MS.gene87039.t1 MTR_1g014180 52.688 186 78 5 16 196 5 185 4.85e-60 186
MS.gene87039.t1 MTR_1g014150 45.361 194 97 5 4 195 2 188 4.52e-47 153
MS.gene87039.t1 MTR_1g014160 40.426 188 87 6 26 195 59 239 1.57e-29 109
MS.gene87039.t1 MTR_2g103840 30.539 167 108 5 39 201 42 204 9.27e-21 86.7
MS.gene87039.t1 MTR_5g074550 30.556 180 110 6 26 195 17 191 1.74e-18 79.7
MS.gene87039.t1 MTR_2g103850 27.807 187 126 6 21 202 24 206 2.21e-16 74.7
MS.gene87039.t1 MTR_4g010180 25.134 187 133 6 20 202 11 194 4.03e-16 73.9
MS.gene87039.t1 MTR_5g074560 27.168 173 115 6 26 195 20 184 2.80e-13 65.9
MS.gene87039.t1 MTR_0650s0010 26.984 189 126 6 21 198 12 199 1.50e-12 64.3
MS.gene87039.t1 MTR_8g099000 28.421 190 113 9 29 198 45 231 3.56e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87039.t1 AT5G01750 56.500 200 82 2 8 202 15 214 2.46e-86 254
MS.gene87039.t1 AT3G11740 57.368 190 79 2 15 202 2 191 1.40e-78 233
MS.gene87039.t1 AT2G14560 50.538 186 91 1 16 200 7 192 7.32e-66 201
MS.gene87039.t1 AT2G14560 48.241 199 101 2 3 200 55 252 7.28e-65 201
MS.gene87039.t1 AT5G01750 56.329 158 64 2 8 160 15 172 1.34e-63 195
MS.gene87039.t1 AT1G33840 48.619 181 92 1 20 199 31 211 4.02e-62 192
MS.gene87039.t1 AT2G14560 50.340 147 72 1 16 161 7 153 4.31e-51 163
MS.gene87039.t1 AT2G14560 48.408 157 79 2 6 161 58 213 3.13e-50 163
MS.gene87039.t1 AT3G16900 42.703 185 86 4 16 199 15 180 9.71e-47 152
MS.gene87039.t1 AT1G33840 46.853 143 75 1 20 161 31 173 2.66e-46 151
MS.gene87039.t1 AT3G56180 34.536 194 120 4 12 201 14 204 5.21e-34 120
MS.gene87039.t1 AT3G56180 34.021 194 119 5 12 201 14 202 9.46e-32 114
MS.gene87039.t1 AT3G10986 32.632 190 121 4 15 197 3 192 1.13e-29 108
MS.gene87039.t1 AT3G15810 28.313 166 113 3 39 202 44 205 2.63e-21 87.8
MS.gene87039.t1 AT1G80120 31.953 169 108 4 39 203 30 195 1.23e-17 77.8
MS.gene87039.t1 AT2G05910 29.586 169 98 7 39 198 28 184 1.73e-13 66.6
MS.gene87039.t1 AT2G38640 30.178 169 109 5 33 198 30 192 1.90e-13 66.6
MS.gene87039.t1 AT5G20640 27.624 181 114 6 36 201 33 211 1.83e-11 61.2

Find 45 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATCACAAACATCTTCTT+TGG 0.257604 7.4:+32415886 None:intergenic
CTTGCGACGTAGGGTGGTTA+TGG 0.262386 7.4:+32416630 None:intergenic
GGATTTGGCGGTCGTCAAAA+AGG 0.341778 7.4:-32416773 MS.gene87039:CDS
TTGCGACGTAGGGTGGTTAT+GGG 0.360381 7.4:+32416631 None:intergenic
ACAATATTGAGAGCCGAATA+TGG 0.380388 7.4:+32416810 None:intergenic
GTTGTCATTGACATCTTTAA+CGG 0.384141 7.4:+32416726 None:intergenic
AGCTAGAAATACATCTAGTT+TGG 0.391033 7.4:+32415916 None:intergenic
TGTTCCGTATCCGGTGGATT+TGG 0.392757 7.4:-32416788 MS.gene87039:CDS
TCAATATTGTGTTCCGTATC+CGG 0.406094 7.4:-32416797 MS.gene87039:CDS
GGGGTGGGTGCTGGCATCGG+TGG 0.406707 7.4:+32416835 None:intergenic
AAAGTCAAAGGTAGTTGGTT+GGG 0.426168 7.4:-32415863 MS.gene87039:CDS
CGATCTTGTATTGTTTATGC+TGG 0.469168 7.4:-32415839 MS.gene87039:CDS
ACCGCCAAATCCACCGGATA+CGG 0.483412 7.4:+32416784 None:intergenic
TTGACGACCGCCAAATCCAC+CGG 0.483926 7.4:+32416778 None:intergenic
AAACTAGATGTATTTCTAGC+TGG 0.502773 7.4:-32415914 MS.gene87039:CDS
GATAAACAGTGACCGCGAAA+TGG 0.505710 7.4:+32415642 None:intergenic
TAAAGTCAAAGGTAGTTGGT+TGG 0.516280 7.4:-32415864 MS.gene87039:CDS
TCCGTATCCGGTGGATTTGG+CGG 0.516653 7.4:-32416785 MS.gene87039:CDS
CCTCTGTGACCACCGTGTCT+TGG 0.523038 7.4:-32416671 MS.gene87039:CDS
AACGGTGAAGTTGTCTGCCA+TGG 0.529412 7.4:+32416744 None:intergenic
AATTGATCAAGACCATTTCG+CGG 0.543054 7.4:-32415654 MS.gene87039:CDS
TGAAAGTACCTTGCGACGTA+GGG 0.546082 7.4:+32416621 None:intergenic
CGTGTCTTGGTTGATGCTGT+TGG 0.551048 7.4:-32416658 MS.gene87039:CDS
TGTTGATTACGCTTTCATAG+TGG 0.551491 7.4:-32415615 MS.gene87039:CDS
TATTGAGAGCCGAATATGGT+GGG 0.560893 7.4:+32416814 None:intergenic
TAGTAACAACATTGTTGCAC+AGG 0.564856 7.4:-32415813 MS.gene87039:intron
TTGAAAGTACCTTGCGACGT+AGG 0.577009 7.4:+32416620 None:intergenic
AGTGAGTATGCATGATCGGT+GGG 0.580863 7.4:-32416017 MS.gene87039:CDS
TATGAAAGCGTAATCAACAC+TGG 0.595693 7.4:+32415619 None:intergenic
CATAACCACCCTACGTCGCA+AGG 0.600007 7.4:-32416629 MS.gene87039:intron
AAGTACCTTGCGACGTAGGG+TGG 0.605892 7.4:+32416624 None:intergenic
ATATTGTGTTCCGTATCCGG+TGG 0.613508 7.4:-32416794 MS.gene87039:CDS
TAGTGAGTATGCATGATCGG+TGG 0.614019 7.4:-32416018 MS.gene87039:CDS
ATTGAGAGCCGAATATGGTG+GGG 0.617660 7.4:+32416815 None:intergenic
AACGTCGTGTTCAAAGTGAA+AGG 0.621566 7.4:-32416706 MS.gene87039:CDS
TTGAGAGCCGAATATGGTGG+GGG 0.626700 7.4:+32416816 None:intergenic
AGATAGTGAGTATGCATGAT+CGG 0.635485 7.4:-32416021 MS.gene87039:CDS
CAAGACACGGTGGTCACAGA+GGG 0.636102 7.4:+32416672 None:intergenic
ATGAAAGCGTAATCAACACT+GGG 0.644614 7.4:+32415620 None:intergenic
TGCGACGTAGGGTGGTTATG+GGG 0.653394 7.4:+32416632 None:intergenic
CCAAGACACGGTGGTCACAG+AGG 0.661785 7.4:+32416671 None:intergenic
ATATTGAGAGCCGAATATGG+TGG 0.662337 7.4:+32416813 None:intergenic
CAACAGCATCAACCAAGACA+CGG 0.674118 7.4:+32416659 None:intergenic
GATCGGTGGGAAGCTTACAG+AGG 0.712766 7.4:-32416004 MS.gene87039:CDS
CAGCATCAACCAAGACACGG+TGG 0.792973 7.4:+32416662 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATATTTTTTCTTTTTTTC+TGG + chr7.4:32416280-32416299 None:intergenic 10.0%
!!! ATATAATGTTTTACTTTGAT+TGG - chr7.4:32416664-32416683 MS.gene87039:CDS 15.0%
!!! TTTTATAAAACATAACTAGT+AGG - chr7.4:32415919-32415938 MS.gene87039:CDS 15.0%
!! TTGAGATATATGAAGTATAA+TGG + chr7.4:32416341-32416360 None:intergenic 20.0%
!!! AAAAAAATTGTTTTGCTCAA+TGG - chr7.4:32416212-32416231 MS.gene87039:intron 20.0%
!!! GATTCATTAAGTTTTACTAT+TGG - chr7.4:32416377-32416396 MS.gene87039:intron 20.0%
! AAAATCACAAACATCTTCTT+TGG + chr7.4:32416541-32416560 None:intergenic 25.0%
! ATACTCTTGTATATACTAGA+TGG + chr7.4:32416646-32416665 None:intergenic 25.0%
! ATCATCTACTAAATTATGAG+TGG + chr7.4:32416112-32416131 None:intergenic 25.0%
! TTTGATTGGATTATCACTTA+AGG - chr7.4:32416678-32416697 MS.gene87039:CDS 25.0%
!!! ATTTTAAAGTCAAAGGTAGT+TGG - chr7.4:32416556-32416575 MS.gene87039:intron 25.0%
!!! GTTTGTGATTTTAAAGTCAA+AGG - chr7.4:32416549-32416568 MS.gene87039:intron 25.0%
AGCTAGAAATACATCTAGTT+TGG + chr7.4:32416511-32416530 None:intergenic 30.0%
GTTGTCATTGACATCTTTAA+CGG + chr7.4:32415701-32415720 None:intergenic 30.0%
!! AAACTAGATGTATTTCTAGC+TGG - chr7.4:32416510-32416529 MS.gene87039:intron 30.0%
AAAGTCAAAGGTAGTTGGTT+GGG - chr7.4:32416561-32416580 MS.gene87039:intron 35.0%
AATTGATCAAGACCATTTCG+CGG - chr7.4:32416770-32416789 MS.gene87039:CDS 35.0%
ACAATATTGAGAGCCGAATA+TGG + chr7.4:32415617-32415636 None:intergenic 35.0%
AGATAGTGAGTATGCATGAT+CGG - chr7.4:32416403-32416422 MS.gene87039:intron 35.0%
CGATCTTGTATTGTTTATGC+TGG - chr7.4:32416585-32416604 MS.gene87039:intron 35.0%
TAAAGTCAAAGGTAGTTGGT+TGG - chr7.4:32416560-32416579 MS.gene87039:intron 35.0%
TAGTAACAACATTGTTGCAC+AGG - chr7.4:32416611-32416630 MS.gene87039:intron 35.0%
TCAATATTGTGTTCCGTATC+CGG - chr7.4:32415627-32415646 MS.gene87039:CDS 35.0%
! ATGAAAGCGTAATCAACACT+GGG + chr7.4:32416807-32416826 None:intergenic 35.0%
! TATGAAAGCGTAATCAACAC+TGG + chr7.4:32416808-32416827 None:intergenic 35.0%
! TGTTGATTACGCTTTCATAG+TGG - chr7.4:32416809-32416828 MS.gene87039:CDS 35.0%
AACGTCGTGTTCAAAGTGAA+AGG - chr7.4:32415718-32415737 MS.gene87039:intron 40.0%
ATATTGAGAGCCGAATATGG+TGG + chr7.4:32415614-32415633 None:intergenic 40.0%
CGTCAAAAAGGTAATGACCA+TGG - chr7.4:32415663-32415682 MS.gene87039:CDS 40.0%
TATTGAGAGCCGAATATGGT+GGG + chr7.4:32415613-32415632 None:intergenic 40.0%
AGTGAGTATGCATGATCGGT+GGG - chr7.4:32416407-32416426 MS.gene87039:intron 45.0%
ATATTGTGTTCCGTATCCGG+TGG - chr7.4:32415630-32415649 MS.gene87039:CDS 45.0%
ATTGAGAGCCGAATATGGTG+GGG + chr7.4:32415612-32415631 None:intergenic 45.0%
CAACAGCATCAACCAAGACA+CGG + chr7.4:32415768-32415787 None:intergenic 45.0%
GATAAACAGTGACCGCGAAA+TGG + chr7.4:32416785-32416804 None:intergenic 45.0%
TAGTGAGTATGCATGATCGG+TGG - chr7.4:32416406-32416425 MS.gene87039:intron 45.0%
!! TGAAAGTACCTTGCGACGTA+GGG + chr7.4:32415806-32415825 None:intergenic 45.0%
!! TTGAAAGTACCTTGCGACGT+AGG + chr7.4:32415807-32415826 None:intergenic 45.0%
AACGGTGAAGTTGTCTGCCA+TGG + chr7.4:32415683-32415702 None:intergenic 50.0%
ACTAGTAGGACATCCGAGTG+TGG - chr7.4:32415933-32415952 MS.gene87039:CDS 50.0%
CAACTACGTGAGTCCACACT+CGG + chr7.4:32415949-32415968 None:intergenic 50.0%
GGATTTGGCGGTCGTCAAAA+AGG - chr7.4:32415651-32415670 MS.gene87039:CDS 50.0%
TTGAGAGCCGAATATGGTGG+GGG + chr7.4:32415611-32415630 None:intergenic 50.0%
TTGCGACGTAGGGTGGTTAT+GGG + chr7.4:32415796-32415815 None:intergenic 50.0%
! CGTGTCTTGGTTGATGCTGT+TGG - chr7.4:32415766-32415785 MS.gene87039:intron 50.0%
! TGTTCCGTATCCGGTGGATT+TGG - chr7.4:32415636-32415655 MS.gene87039:CDS 50.0%
AAGTACCTTGCGACGTAGGG+TGG + chr7.4:32415803-32415822 None:intergenic 55.0%
ACCGCCAAATCCACCGGATA+CGG + chr7.4:32415643-32415662 None:intergenic 55.0%
CAAGACACGGTGGTCACAGA+GGG + chr7.4:32415755-32415774 None:intergenic 55.0%
CAGCATCAACCAAGACACGG+TGG + chr7.4:32415765-32415784 None:intergenic 55.0%
CATAACCACCCTACGTCGCA+AGG - chr7.4:32415795-32415814 MS.gene87039:intron 55.0%
CTTGCGACGTAGGGTGGTTA+TGG + chr7.4:32415797-32415816 None:intergenic 55.0%
GATCGGTGGGAAGCTTACAG+AGG - chr7.4:32416420-32416439 MS.gene87039:intron 55.0%
TGCGACGTAGGGTGGTTATG+GGG + chr7.4:32415795-32415814 None:intergenic 55.0%
TTGACGACCGCCAAATCCAC+CGG + chr7.4:32415649-32415668 None:intergenic 55.0%
! TCCGTATCCGGTGGATTTGG+CGG - chr7.4:32415639-32415658 MS.gene87039:CDS 55.0%
CCAAGACACGGTGGTCACAG+AGG + chr7.4:32415756-32415775 None:intergenic 60.0%
CCTCTGTGACCACCGTGTCT+TGG - chr7.4:32415753-32415772 MS.gene87039:intron 60.0%
GCACCCACCCCCACCATATT+CGG - chr7.4:32415601-32415620 MS.gene87039:CDS 60.0%
! AATATGGTGGGGGTGGGTGC+TGG + chr7.4:32415601-32415620 None:intergenic 60.0%
! AGAGCCGAATATGGTGGGGG+TGG + chr7.4:32415608-32415627 None:intergenic 60.0%
! GAGCCGAATATGGTGGGGGT+GGG + chr7.4:32415607-32415626 None:intergenic 60.0%
!! GTGGGGGTGGGTGCTGGCAT+CGG + chr7.4:32415595-32415614 None:intergenic 70.0%
!! GGGGTGGGTGCTGGCATCGG+TGG + chr7.4:32415592-32415611 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 32415571 32416875 32415571 ID=MS.gene87039
chr7.4 mRNA 32415571 32416875 32415571 ID=MS.gene87039.t1;Parent=MS.gene87039
chr7.4 exon 32416630 32416875 32416630 ID=MS.gene87039.t1.exon1;Parent=MS.gene87039.t1
chr7.4 CDS 32416630 32416875 32416630 ID=cds.MS.gene87039.t1;Parent=MS.gene87039.t1
chr7.4 exon 32415814 32416041 32415814 ID=MS.gene87039.t1.exon2;Parent=MS.gene87039.t1
chr7.4 CDS 32415814 32416041 32415814 ID=cds.MS.gene87039.t1;Parent=MS.gene87039.t1
chr7.4 exon 32415571 32415708 32415571 ID=MS.gene87039.t1.exon3;Parent=MS.gene87039.t1
chr7.4 CDS 32415571 32415708 32415571 ID=cds.MS.gene87039.t1;Parent=MS.gene87039.t1
Gene Sequence

>MS.gene87039

ATGTCTTATTCTTATCAACCACCGATGCCAGCACCCACCCCCACCATATTCGGCTCTCAATATTGTGTTCCGTATCCGGTGGATTTGGCGGTCGTCAAAAAGGTAATGACCATGGCAGACAACTTCACCGTTAAAGATGTCAATGACAACGTCGTGTTCAAAGTGAAAGGTTCTCTCTTAACCCTCTGTGACCACCGTGTCTTGGTTGATGCTGTTGGTAACCCCATAACCACCCTACGTCGCAAGGTACTTTCAACTTTATTAAGAAACTAGTTTTCTTTCAGACTTTTATTTTAATGCTCAATATTTTATTTTTGTGATGAATTTTTTTATATATACGATATTTTATTTTATAAAACATAACTAGTAGGACATCCGAGTGTGGACTCACGTAGTTGTGCGTTGCACTTTATTTTTAGATAAGACGCAGCTTTATTACATTTCAATAATATTTAATATGAAAAATTGTATGTGTTATAAATTTATATATTTTGTAACTTGAAACTCTTTACATAAAAGTTTGTAAGTTAAAAATTATCCACTCATAATTTAGTAGATGATTAATTTTTGAAATCTTTTCAAAGAGAAATATGATATATTTTACTTAGAGCTCATATATAAGTTATTCACTATAAACTTAAAAAAAAATTGTTTTGCTCAATGGAGTCTTATTAGTGTGTGAATTTCAATAATTCGTTGATTGAAACCAGAAAAAAAGAAAAAATATAAATGTTAGAGTAACGTTTATTGTTAAAAAATCATATTATCCATTATACTTCATATATCTCAATAAAGTGTTGTTTAATGATTCATTAAGTTTTACTATTGGTGCAGATAGTGAGTATGCATGATCGGTGGGAAGCTTACAGAGGAGAAAGCACAAATTCGAAAGATCTTATATTTACATTGAGAAAATCATCGCTATTGCAGTTGAAGACCAAACTAGATGTATTTCTAGCTGGTAATACCAAAGAAGATGTTTGTGATTTTAAAGTCAAAGGTAGTTGGTTGGGACGATCTTGTATTGTTTATGCTGGTGATAGTAACAACATTGTTGCACAGGTAGTAAAAACCATCTAGTATATACAAGAGTATATAATGTTTTACTTTGATTGGATTATCACTTAAGGACATATACTAATCATAAATCAATTTTTGTTATTGCAGATGCACAAAAAGCACACTATTAAGAGTATTTTAATTGATCAAGACCATTTCGCGGTCACTGTTTATCCCAGTGTTGATTACGCTTTCATAGTGGCATTAATCGCGATTCTTAACGAGATTAACGAAGATGATGTTTGA

Protein sequence

>MS.gene87039.t1

MSYSYQPPMPAPTPTIFGSQYCVPYPVDLAVVKKVMTMADNFTVKDVNDNVVFKVKGSLLTLCDHRVLVDAVGNPITTLRRKIVSMHDRWEAYRGESTNSKDLIFTLRKSSLLQLKTKLDVFLAGNTKEDVCDFKVKGSWLGRSCIVYAGDSNNIVAQMHKKHTIKSILIDQDHFAVTVYPSVDYAFIVALIAILNEINEDDV